Ayurvedic Herbo-mineral Formulation Research Articles

Role of Shwasakuthar Rasa and Other Herbomineral Formulations with Yoga in Migraine

Ayurveda is an ancient traditional system of medicine to treat illness and encourage well being by using natural whole body healing techniques. Migraine is a primary headache disorder which is characterized by episodic recurrent attacks. As it affects one billion individuals each year across the worldwide has proven to one of the most common neurological disorders with high prevalence and morbidity among the younger age groups. Migraine can be classified on the basis of presence of Aura which shows prodromal symptoms like irritability, behavioural changes, etc and associated symptoms of photophobia, phonophobia and vomiting that usually lasts for 4 to 72 hours. In Ayurveda, migraine is clinically correlated with Ardhavabhedaka which is described as Tridoshapradhana by Sushruta and Vatakaphapradhana by Vagabhata. The management of migraine is done routinely by reducing the symptoms and the episodic recurrent attacks with Ayurvedic approach as modern medicine cause gastric irritation and drug dependency which further triggers the attack. The present case of 35 years old female diagnosed with migraine has shown symptoms of headache involving left side of head associated with nausea, vomiting and sensitivity to light and sound. The patient was treated with Nasya Karma which act as a gateway to head as said by Acharya Charaka and with oral Ayurvedic herbomineral formulation Shamana Chikitsa was done that alleviates the symptoms of Ardhavabhedaka. A significant relief from all symptoms was observed within a period of 15 days, demonstrating the Ayurveda as the best way to improve the overall well being of mind, body and spirit.

Ayurvedic treatment protocol in the management of pancreatitis: A nonrandomized observational study

Recurrent Acute/Chronic Pancreatitis (RA/CP) is a progressively debilitating disease with rising incidences in recent years. The limitations of conventional treatment, along with the psychological fear and financial burden associated with it, compel the patients to explore alternative options. In India, where traditional medicines are recognized as treatment options, a North India-based ayurvedic clinic has been treating RA/CP patients using an ayurvedic Herbo-Mineral Formulation (HMF) with a balanced diet and regulated lifestyle. The HMF is prepared using processed mercury, copper, and sulfur following the principles of Rasashastra. The HMF has demonstrated pancreatitis preventive properties in rat models and passed acute, subacute, and chronic toxicity assessments. This retrospective study enrolled 1750 well-diagnosed cases of RA/CP from January 1997 to July 2023. About 67% of the enrolled patients were nonalcoholics, 81% were nontobacco users, and 93% had no family history of the disease. The age group of 19–45 years represented the highest proportion of patients, with a male predominance (5:1). Nine hundred and sixteen patients with RA/CP completed 1-year ayurvedic intervention using HMF, without pancreatic enzymes. The ayurvedic treatment resulted in a significant 93% reduction in the frequency of pancreatitis attacks and a 97% decrease in emergency hospitalizations. The HMF has shown no adverse effects or toxicity in the treated patients. About 1.7% of patients experienced mortality during the treatment or follow-up period due to various reasons. The ayurvedic treatment protocol demonstrated sustainable effects, with the longest remission exceeding 26 years, and has brought a significant reduction in frequency and intensity of RA/CP attacks with an overall improvement in quality of life, warranting further randomized clinical trials to establish strong proof of efficacy.

A Review on Probable Mode of Action of Gandhaka Rasayana - An Ayurvedic Herbo-Mineral Formulation with Multifaceted Action

As per ancient Ayurvedic scholars for getting a desirable outcome of any medication, it should be precisely analysed before prescribing to the patient. There are numbers of classical formulations successfully practiced by Ayurvedic physicians for treating various ailments but in maximum cases there is lack of data regarding their details mechanism of action. Such one multidrug classical formulation is Gandhaka rasayana which is widely practiced Ayurvedic formulation. Gandhaka rasayana have been used in Ayurvedic Rasashastra to treat various types of diseases related to Tvak, Mandhagni, various Srotas. It is a time tested formulation used by Ayurvedic practitioners for correcting Kushta, Indralupta, Vatarakta, Palitya, Dushta vrana, Arumsika etc. Modern scientific world needs explanation and logic behind the mode of action of Gandhaka rasayana with respective to each particular disease. This review is an attempt to explain multiple mode of action of Gandhaka rasayana in various diseases on analysing properties of ingredients. Analysis of mode of action gives deep insight about use of formulation with a better understanding.

Ayurvedic Management of Pancreatitis

Pancreatitis is the life threatening metabolic pathology of the gastrointestinal system. It progressively damages the pancreas and result in the loss of its functions. The prevalence rate of pancreatitis in India is 7.9 per 10, 0000. For male and female respectively 8.6 and 8 in India. With overall 10 year and 20 year survival rates are estimated to be about 70% to 45% respectively. In the contemporary science the treatment protocol is limited to painkillers, intravenous fluids and pancreatic enzymes. Despite of advance techniques in the allopathic science the prognosis of the disease is unclear. Ayurvedic formulations of mineral complex including a diet and moderate rest have shown excellent result. In this case report a 40 year old male patient a known case of chronic pancreatitis with recurrent abdominal pain which radiates to back, nausea, vomiting, tenderness, anorexia, along with elevated serum amylase level was managed effectively with Ayurvedic medications by the Department of PG Study in Shalyatantra OPD Dr. BRKR Government Ayurvedic Medical College and Hospital, Hyderabad. In Ayurveda this condition can be correlated Agnashayashoth which comes under Pittodara. And it was successfully treated with the medications like Shankha vati, Kravyadi ras, Arogyavardgani vati. Within 4 months of treatment patient become normal. There was no pain abdomen, nausea, vomiting, bloating of abdomen. And serum amylase came to its normal level. This shows that Ayurvedic herbo mineral formulations showing promising result in the management of pancreatitis.

Conceptual Study on Ayurvedic Pharmacodyanamics of Gandhaka Rasayana wsr to Grahani Roga

Vitiation of Agni (Mandagni) and Dosha cause Grahani Roga, a chronic illness of the digestive system that is closely tied to Agni state and dietary patterns. Gandhaka Rasayana, a popular Ayurvedic herbo-mineral formulation, was created by Shuddha Gandhaka through the serial Bhavana of eleven herbal medications eight times apiece. It is widely used to treat a wide range of gastrointestinal, skin, and wound ailments. An effort was made in this paper to prove properties and capabilities of Gandhaka Rasayana with special reference to Grahani Roga’s management.

Nomenclature of Aushadh Sevan Kaal(Time of Drug Administration) In Various Ayurved Samhita’s and Its Commentaries- A Critical Review

Aushadh Sevan Kaal is a concept described in Ayurved samhitas, which means the time of medicine administration.Kaal(Time) isincluded in karana dravya and plays many important roles in our lives. The existence of the Kaal is from the Shrishti Uttpatti.Aacharya charak hasdescribed Trisutra Ayurved, which explains the knowledge about the entire Ayurveda.Trisutra includes Hetu[causative factors], Linga[signs andsymptoms], and Aushadh [medicines]. Additional considerations listed by Acharya Charaka include the patient's desha (region), kala (time),pramana (dosage), satmya (wholesomeness), asatmya (unwholesomeness), aahara (diet), and aushadh (medicine). Kaal is a particular and distinctcause of all kinds of outcomes and is also inevitable. Ayurveda is the science of life, emphasizing the idea of kaal as it is one of the karan dravya.The present study aims to critically analyze different terms used in samhitas for nomenclature and terminologies related to the administration time(aushadh sevan kaal) of ayurvedic herbo mineral formulations. To achieve this, we aim to study the concept of aushadha sevan kaal in varioussamhitas for its utility in identifying textual study material for selecting the treatment protocol. There are differences in the nomenclature[terminologies] in various samhitas and their commentaries. Mild variation is also observed in the number of types of Aushadh sevan kaal as perSamhitas. All Ayurveda treatments concentrate on maintaining and retaining the power of agni because it is the primary factor in establishing theDhatu Samyata.In the current era, it is observed that proper medicine administration needs to be done in relationship with Dosha,dusya,rogi, androgbala in consideration of the aushadh seven kaal. Hence, treatment is not successful in treating the patients.In this study, it is observed thatdifferent terms are mentioned in Ayurveda texts for different types of Aushadh sevan Kaal by different-different Acharyas.

Clinical Evaluation of a New Ayurvedic Herbo-Mineral Formulation (NIA/DG/2020/01) in Stage -1 Primary Hypertension

Hypertension, also known as high or raised blood pressure, is a multifactorial disease with multiple causes and multiple treatments. In India, the prevalence of hypertension was estimated to be 40.8% in urban areas and 17.9% in rural areas. Though there are multiple treatment of disease still it is a challenge to manage this disease effectively with lower side effects as all available treatments are full of side effects and toxic effects. New Ayurvedic herbo-mineral formulation NIA/DG/2020/01 is a new anti-hypertensive Ayurvedic Ghan formulation, containing Arjuna (Terminalia arjuna Roxb.), Ashwagandha (Withania somnifera Linn.), Jatamansi (Nordostachys jatamansi DC.), Shankhpushpi (Convolvulus pluricaulis Chois.), Punarnava (Boerhavia diffusa Linn.), Gojihwa (Onosma Bracteatum Wall.), Guduchi (Tinospora cordifolia Willd.), Mukta Shukti (Margarita) and Praval Pisti (Corrallium Rubrum). Aim and Objective: To assess role of a new Ayurvedic herbo-mineral formulation NIA/DG/2020/01 in Stage-1 primary hypertension. Material and Methods: Initially, the formulations were prepared by the instructions mentioned as per classical text. Then clinical trial was proceeded after ethical clearance and CTRI registration on 60 patients from NIA Arogyashala as per inclusion-exclusion criteria and other said parameters. Observation and Results: Recorded systolic and diastolic blood pressure was tabulated and analysed statistically by SPSS in both the trial groups. Discussion: The result of systolic and diastolic blood pressure changes and percentage of relief was discussed thoroughly. Conclusion: The trial drugs showed highly significant reduction in Blood pressure of stage -01 Hypertension when given at a dose of 1 capsule twice a day before meal for 90 days.

Antiviral Activity of Svarnvir-IV Tablet Assayed for Activity Against SARS-CoV-2 In Vitro.

The coronavirus disease (COVID-19), caused by the virus SARS-CoV-2, has become a global pandemic in a very short time span. While several vaccines have been developed in the last year, specific treatments for CoV infection are still being explored. Thus, the situation highlights the need to develop safe and efficacious antiviral therapeutics. AyurvedicRasayanatherapy has been traditionally used in India for its holistic healing systems and proven history of empirical use. There is emerging evidence that Ayurvedic treatment methodologies and herbal medicines may be effective strategies in combating COVID-19. The present study is aimed at evaluating the antiviral and therapeutic activity of an Ayurvedic herbomineral formulation (Svarnvir-IV tablet, 450 mg) against the SARS-CoV-2 virusin vitro. A cell-based assay was conducted to evaluate the cytotoxicity of the Svarnvir-IV tablets (Aimil Pharmaceuticals, Delhi, India) for the determination of virucidal activity assessment (at 2 hours) and therapeutic activity assessment (at 1 hour, 2 hours, and 4 hours). When incubated with SARS-CoV-2 virus at 0.1 multiplicity of infection (MoI) for two hours, Svarnvir-IV tablet exhibited virucidal activity against SARS-CoV-2 with an EC50 value of 0.0058 mg/ml. It also exhibited therapeutic activity when treated with cells infected with the SARS-CoV-2 virus (0.1 MoI) for 1 hour, 2 hours and 4 hours post-infection, with an EC50 value of 0.094 mg/ml, 0.023 mg/ml, and 0.05 mg/ml, respectively. The original supporting data obtained from this study, along with existing Ayurvedic traditional information, has shown encouraging results.

Evaluation of the anti-arthritic activity of Rhuflex-F - A proprietary Ayurvedic herbomineral formulation in albino rats.

Rhuflex-F is a proprietary Ayurvedic herbo-mineral formulation clinically used to combat and relieve stiffness in joints and muscles, reduce edema, restore mobility, and also effective in relieving the symptoms of other autoimmune illnesses that lead to rheumatism. The aim and objective of the research study is to evaluate the efficacy of Rhuflex-F against in vitro protein denaturation and in vivo Freund's adjuvant-induced arthritis in albino rats. In vitro inhibition of protein denaturation activity was carried out using bovine serum albumin. For in vivo activity, arthritis was induced by complete Freund's adjuvant in albino rats. Rhuflex-F (135-270 mg/kg, po) was administered for 30th days in arthritic rats, and effects were assessed on primary and secondary paw edema, on pain response, hematological, serum biochemical parameters (serum transaminases, alkaline phosphatase, urea, uric acid, and orosomucoid), and serum anti-oxidant parameters and adrenal ascorbic acid. Aqueous extract of Rhuflex-F showed in vitro protein denaturation inhibitory activity in a dose-dependent manner. Rhuflex-F showed nonsignificant decrease in primary and secondary paw edema with reduced pain response, some reversal effects on hematological parameters such as white blood cell and red blood cell related parameters and serum orosomucoid and adrenal ascorbic acid in comparison to Fruend's adjuvant control group. Further, Rhuflex-F reversed Freund's adjuvant-induced adverse effects on oxidant status in the serum of albino rats. Result of the present study suggested that Rhuflex-F formulation has anti-inflammatory activity, may be due to the inhibition of protein denaturation in vitro and in vivo anti-arthritic activity against complete Freund's adjuvant-induced arthritis in albino rats.

Evaluation of chronic toxicological profile of herbo-mineral formulations: Shwaskas Chintamani Rasa and its marketed formulation namely Kas Shwas Hari Rasa

BackgroundShwaskas Chintamani Rasa (SKC) and Kas Shwas Hari Rasa (KSH) are the Ayurvedic herbo-mineral formulations. These Ayurvedic formulations contain heavy metals which is the reason of concern and might bring up the safety issue. ObjectiveThis research article is aimed to study chronic toxicity of SKC and KSH for safety aspect in Wistar rats. Material and methodA study group of 220 healthy rats were divided into six groups. These rats were administered with SKC and KSH formulations where both the formulations were administered for 180 consecutive days. SKC was administered at doses of 58 mg/kg (equivalent to therapeutic dose i.e. TD), 145 mg/kg (2.5 TD), 290 mg/kg (5 TD) and KSH was administered at dose of 58 mg/kg (TD). According to OECD guideline 452, the effect of these formulations was examined on hematology, serum biochemistry and histopathology of various organs. ResultsBoth the formulations did not produce any signs or symptoms of treatment related toxicity in both male and female Wistar rats at therapeutic dose (TD), 2.5 times TD and 5 times TD. ConclusionBased on these findings, the NOAEL (No observed adverse effect level) for test formulations SKC and KSH tablets in male and female wistar rats concluded to be preclinically safe.

Nanoscale Diamond-Based Formulation as an Immunomodulator and Potential Therapeutic for Lymphoma.

Integrative medicine practices, such as Ayurveda, are popular in India and many South Asian countries, yet basic research to investigate the concepts, procedures, and medical benefits of ayurvedic products has received little attention and is not fully understood. Here, we report a functional nanodiamond-based traditional Ayurvedic herbomineral formulation, Heerak Bhasma (Ayu_ND), for the treatment of solid tumors called Dalton’s lymphoma generated in CD1 mice. Ayu_ND-mediated immunostimulation significantly reduces tumor cell proliferation and induces apoptosis aided by the active participation of dendritic cells. Immunomodulatory Ayu_ND treatment is highly immunostimulatory and drives dendritic cells to produce TNF-α. Treatment with Ayu_ND significantly reduces the tumor volume, inhibits metastasis in distant vascularized organs, and increases the life span of tumor-bearing animals compared with untreated littermates. These events were associated with elevated serum levels of the protective cytokines IFN-γ and TNF-α and downregulated the disease, exacerbating TGF-β. Ayu_ND-mediated therapeutic success was also accompanied by the depletion of regulatory T cells and enhanced vaccine-induced T-cell immunity, guided by the restoration of the memory CD8+ T-cell pool and prevention of PD-1-mediated T cell exhaustion. The results provide a basis for further evaluation of ayurvedic formulations and drug efficacy in treating cancers.

Efficacy and safety of Trikgudas an add-on medicine in uncontrolled type 2 diabetes mellitus (T2DM) patients with metformin monotherapy: A randomized controlled trial

Metformin is quite effective in managing Type 2 diabetes mellitus (T2DM). Studies have reported that the combined use of oral hypoglycemic medicines is prescribed in uncontrolled T2DM metformin monotherapy. So, we aim to evaluate the efficacy and safety of Ayurvedic herbo-mineral formulation (Trikgud) in managing uncontrolled T2DM with metformin monotherapy in Nepalese population. This study was a randomized, double-blind, and controlled trial. A total of 56 participants with the diagnosis of T2DM were randomly assigned to the intervention group (Trikgudmet) or control (Met) group in a ratio of 1:1 for 12weeks. The primary outcome measure was was the difference in the change in glycated haemoglobulin (HbA1C), fasting plasma glucose (FPG) and 2-h postprandial plasma glucose (2-h PPG) between the groups, and secondary outcome measures was the change in body mass index (BMI), waist-hip ratio (WHR), lipid profile, liver function tests and renal function tests between the baseline and at 12weeks after intervention. At week 12, there were significant differences of FPG and 2-h PPG in both Trikgudmet group and Met group when compared with week 0. There was a statistically significant decrease of 8.6% and 10.1% of HbA1C levels in Trikgumet and Met groups, respectively. This study may provide new evidence for use of Trikgudas an add-on therapy for uncontrolled T2DM patients with metformin monotherapy in context of Nepal.

Efficacy of Ayurvedic herbal formulation and Ayurvedic herbo-mineral formulation in hypothyroidism patients

Background: Hypothyroidism is one of the major endrocrine disorders seen in general population worldwide. Although modern drugs are quite effective in managing hypothyroidism, it is subject to sustaining certain side effects. So, we aim to evaluate the efficacy of Ayurvedic herbal formulation and Ayurvedic herbo-mineral formulation in managing hypothyroidism in Nepalese population. Methods and analysis: This study was a randomized, double-blind, and controlled trial. A total of 50 participants with the diagnosis of hypothyroidism was randomly assigned to the intervention group or control group in a ratio of 1:1 for 4 weeks. The primary outcome measure was the thyroidstimulating hormone level, and secondary outcome measures was the change in body mass index, waist-hip ratio, blood glucose level, lipid profile, liver function tests and renal function tests between the baseline and at 4 weeks after intervention. Statistical analysis was done by comparing categorical variables using chi-square test and Fisher’s exact test and comparing continuous variables using paired and unpaired student’s t test. Results: There was a decrease in TSH levels of the participants by 12.5% and 7.9%, after intervention with AHF and AHMF, respectively. There were a small statistical significant difference only in waist-hip ratio ( p= 0.04) when compared before and after treatment with AHF and AHMF whereas there was no significant changes observed in other clinical variables after intervention with AHF and AHMF. Conclusion: This study may provide new evidence for the effectiveness of Ayurvedic herbal formulation and Ayurvedic herbo-mineral formulation in hypothyroidism in context of Nepal.

Pharmaceutical and Analytical Study of Tryushanadya Lauha & Modified Form as Tryushanadya Mandura and their Comparative Evaluation for Antidiabetic Activity in Wistar Rats

Background: Lohais a metal that is used in many preparations after transforming it into non-metallic form by purification and incineration method uses to treat different kinds of diseases. Mandura is the rusting of iron.Tryushanadyalauha(TL) is one among the Ayurvedicherbo-mineral formulations described in BhaishajyaRatnavalliand as modified dosage form as Tryushanadyamandura(TM).The herbal contents are tryushana (i.epippali( Piper longum Linn ),maricha (Piper nigrum Linn)and shunti (Zingiber Officinale Roscoe), cavya (Piper chabaHunter), bakuchi (Psoralea CorylifoliaLinn),bhang (cannabis sativum Linn),andlavana like saindhava,aubhida,vida and sauvarchala ,andlohabhasma is the main ingredient.
 Aim: Pharmaceutical and Analytical study of TryushanadyaLauha& modified form as TryushanadyaMandura and comparative evaluation for antidiabetic activity in Wistar rats.
 Materials and Methods: All herbal drugs will be collected, verified, and primarily authenticated by the Department of Dravyaguna. Lohaand Mandura will be procured from the vendor and authenticated by the Department of Rasashastra and BhaishajyaKalpana, Mahatma Gandhi Ayurved College Hospital Research Centre, Salod(H), Wardha, and they will be prepared as per reference. Organoleptic, bhasmapariksha, Physico-chemical,XRD and FEG-SEM parameters will be evaluated. To assess Tryushanadya Lauha (TL) and Tryushanadya Mandura (TM)antidiabetic action will be conducted in 30 Wistar rats in 5 groups and will be compared.
 Observation and Results: The study will be assessed Tryushanadya lauha (TL) and TryushanadyaMandura (TM)antidiabetic action in 30 Wistar rats by using one-way ANOVA.
 Conclusion: Pharmaceutical and Analytical study of TryushanadyaLauha (TL) & modified form as TryushanadyaMandura (TM) will provide the standard parameters.

A critical Review of Dhoopan Karma in Ayurveda and Dhoopana of an Ayurvedic Herbomineral formulation Yavasarshapadi churna in context of Sterilization.

Dhoopana Karma is classical remedy mentioned in various Ayurvedic scriptures to maintain disease free and hygienic environment in the world. As per Ayurvedic scriptures Dhoopana is indicated for Rakshoghna Karma which means killing of disease producing microorganisms. Hence, it plays key role in prevention and cure of various infectious diseases.A similar process called fumigation is one of best and fastest method to maintain asepsis, antisepsis in wards and operation theatres of hospitals.Additionally, this study comprises critical literary review of herbomineral formulation named Yavasarshapadi Choorna which is indicated in form of incense for Dhoopana karma. It is indicated in Ayurvedic scripture Charaka Samhita in chapter Jatisutriya Sharir Adhyaya of Sharir Sthana. It was reviewed in context of sterilization activity to maintain asepsis and antisepsis. Specifically, it is indicated for fumigation of cloths and belongings of post-natal period of Women and that of the New born, Drug preparation room, New born care room, Surgical Procedure room modern surgery, traditional operation theatre sterilization was done by formalin gas fumigation over many decades which is found to have carcinogenic effect over human lives. Ayurvedic modality named Dhoopana was undertaken in current study as it is a safe and healthy alternative for sterilization.It overcomes harmful effects caused by modern sterilization method i.e. Formalin gas fumigation which is need of an hour. The Analytical study of antimicrobial and antifungal effect of various Ayurvedic Dhoopana Dravyas is the need of the hour along with preclinical and clinical study to verify Sterilization activity of various Ayurvedic Dhoopana Dravyas.

Effect of Triphala Kajjali tablet - an Ayurvedic herbomineral formulation for the management of metabolic syndrome: a single case study

Metabolic syndrome (MS) is a group of disorders that includes abdominal obesity, diabetes, hypertension, and elevated cholesterol. Approximately 25% of the adult populations are affected by MS. Gradually this number is increasing because of poor lifestyles, faulty dietary pattern, physical inactivity, stressful life and rapid urbanization. A 50-year-old female patient visited to OPD of Kayachikitsa department ITRA, Jamnagar, with the complaints of gradual weight gain with excess body fat around the abdomen and waist region, numbness and burning sensation at bilateral feet, and breathlessness on exertion. After investigation, she was diagnosed as a case of MS as per National Cholesterol Education Program (NCEP), adult treatment panel III (revised in 2005) guideline. She was treated with Triphala Kajjali tablet for 12 weeks along with lifestyle modification. After completion of treatment, investigations revealed reduction in fasting blood sugar from 113 mg/dL to 80 mg/dL, serum cholesterol level was decreased to 148 mg/dl from 216 mg/dl, serum triglycerides level was reduced to 150 mg/dL from 187 mg/dL and serum LDL was reduced to 68.3 mg/dl from 118.7 mg/dl. Her weight reduced to75 kg from 85 kg, and waist circumference reduced to 92 cm from 100cm, and blood pressures also reduced up to 122/82 mmHg from 140/90 mmHg. Hence, it can be concluded that Triphala Kajjali tablet along with lifestyle modification are effective in the management of MS, as it possesses Kapha Medohara properties.

Impact of Modern Analytical Tools In The Standardization of Kharaliya Rasayana (Arogyavardhini Rasa)

Background: Arogyavardhini Rasa is a well known Ayurvedic herbo-mineral formulation widely used for a broad spectrum of therapeutic indications mainly in the treatment of Kamala (jaundice) and other Yakrit Vikara (liver disorders).
 Aim: To standardized Arogyavardhini Rasa prepared with and without Rasavarga Dravya (ingredients of Metallo-mineral origin) by using high-performance thin-layer chromatography (HPTLC) & computational by principal component analysis (PCA) as an analytical tool. 
 Materials & Method: Arogyavardhani Rasa (AVR) was prepared as per classical reference and further modified this preparation by omitting Rasavarga dravya (ingredients of Metallo-mineral origin) of Arogyavardhani Rasa (MAVR). Formulations were investigated for physicho-chemical parameters, chromatography and using the building block of chemometrics technique such as PCA was applied to discriminate the formulations AVR and MAVR.
 Result & Discussion: HPTLC study revealed a total of 11 and 8 bands at 254 nm and 366 nm in both AVR & MAVR, with only two similar Rf values in the sample. PCA revealed that both formulations were found similarities & dissimilarities within a batch of AVR and MAVR with its physicho-chemical constant.
 Conclusion: The analytical tools applied (HPTLC and PCA) for the study revealed that the classical processing like Bhavana (Lavigation ), Mardana (Trituration) etc. had a significant role in the preparation of Rasaaushadhis (mercurial medicine) and process validate the Indian system of medicinal pharmaceutics.

Comparative Evaluation of Antipyretic Activity of an Ayurvedic Herbo-mineral Formulation Dhatryadi Churna and Its Modified Dosage form in Albino Wistar Rats

Introduction: Experimental studies are essential and expected part of a new drug development. The studies are conducted to know about the toxic effects (if any), produced by the drug & to access safety of the drug. Studies also help to ascertain the efficacy of the drug & measures to be taken so as to curtail the toxic effects.
 Aim & Objective: Study was aimed to do comparative evaluation of antipyretic activity of an Ayurvedic herbo-mineral formulation Dhatryadi Churna and its modified dosage form in experimental albino rats.
 Materials and Methods: Present study was carried out at Animal house attached with the Institute. 24 healthy Albino wistar rats of either sex weighing 180-200 gm were randomly selected and divided into 4 groups. Pyrexia was induced in rat by subcutaneous injection of 10 mL/kg b.w. of a 20% aqueous suspension of brewer's yeast. The trial drug Dhatryadi Churna and Dhatryadi Vati administered orally in the form of suspension to the albino rats.
 Observation and Results: The present result shows that the Dhatryadi Churna (DC) and Dhatryadi Vati (DV) have a significant antipyretic effect in yeast induced pyrexia in rats. Hematological parameters were found to be within the normal biological and laboratory limits on comparison with the values of the control group.
 Conclusion: Dhatryadi churna and Dhatryadi Vati does not show significant adverse effects on the blood, blood cells, and target organs at the doses used and can be safely used in human being.

Anti-inflammatory and analgesic activities of Vatavidhvamsana rasa, An Ayurvedic herbo-mineral formulation

Background: Inflammation is a complex process mainly comprises of three phases namely acute, sub-acute and chronic. In acute inflammatory conditions fluid and granulocytic cells accumulate at the site of injury because of the changes in small blood vessels. This response often activates systemic response such as leucocytosis, protein catabolism, fever and synthesis of C-reactive protein, an acute-phase protein In Ayurvedic texts, several herbo-mineral preparations to fight against inflammation have been found; among them, Vatavidhvamsana Rasa (VVR) is one, which is said to be remarkable in inflammation condition. Aim and Objectives: To assess the anti-inflammatory and analgesic effects of VVR in pharmacologically validated models. Materials and methods: Wistar strain albino rats weighing 200 ± 20 of either sex were used in the study. VVR was administered at a dose of 22.5 mg/kg for the rat. Results: Vatavidhvamsana Rasa (VVR) showed a significant decrease in the carrageenan-induced paw oedema after 1-hour interval in comparison to the control group (P<0.05) and at 3 hours and 5 hours intervals it showed a non-significant decrease in carrageenan-induced paw oedema in albino rats. In the analgesic activity, VVR treated group showed a significant increase in radiant heat response at 30 min. interval (P<0.05) and non-significant increase at 60 mins in comparison to initial and control group. Conclusion: The results concluded that Vatavidhvamsana Rasa has anti-inflammatory and analgesic activity. Accordingly, it can be used in the management of pain and inflammatory conditions.

Pharmacognostical and pharmaceutical analysis of Triphala Kajjali tablet-an ayurvedic herbomineral formulation for metabolic syndrome

Background: Triphala is one of the most easily available and commonly used medicine which contains fine powder of three fruits viz. Terminalia chebula Retz. (Haritaki), Terminalia bellerica Roxb. (Bibhitaki) and Embilica officinalis Gaertn. (Amalaki) and is indicated as one of the drugs for management of disorders of Kapha and Meda. Due to bitter and astringent taste of Triphala in the powder form is a major complaint of the patients. So, to discover that form of Triphala, which is easy to take, effective in low dose, has long shelf life and simple to dispense is the need. For assurance of quality of herbal compounds pharmacognostical and pharmaceutical analysis should be done. Methods: Triphala Kajjali was subjected to microscopic evaluation for pharmacognostical, physiochemical analysis like hardness, weight variation, loss on drying, ash value, acid insoluble extract, pH value, water soluble extract, alcohol soluble extract and high-performance thin layer chromatography (HPTLC).Results: Pharmacognostical study showed the presence of certain identifying characters of all of the ingredients of Triphala Kajjali that is Haritaki, Bibhitaki and Amalaki. In pharmaceutical study, preliminary physiochemical analysis showed that hardness of the tablet was 2.05 kg/cm2, ash value 4.03%w/w, loss on drying 5.5%w/w, water soluble extract 5.89%w/w, alcohol soluble extract 25.96%w/w and HPTLC showed 7 spots in 254 nm and 7 spots in 366 nm.Conclusions: Pharmacognostical and physico-chemical observations revealed the specific characters of all active constituents of Triphala Kajjali and confirmed the purity and genuinity of the drug.