The complexity of technologies in modern aircraft makes their maintenance a critical function. The work environment is diverse and dynamic, with uninterrupted working hours and multiple demands, which can result in inadequate sleep and excessive workload for Aircraft Maintenance Technicians. This causes fatigue and increases the likelihood of human errors, directly affecting safety. A study was conducted with 312 participants to investigate how fatigue affects maintenance professionals. Participants responded to questionnaires that included four culturally validated scales to measure levels of fatigue, sleepiness, workload, and quality of life. Statistical analyses were performed to correlate sociodemographic factors and work-related aspects with levels of fatigue. The results revealed a positive and significant correlation between fatigue, sleepiness, and workload, and a significant negative correlation with quality of life. It was found that 52.9% of participants experienced some level of fatigue, highlighting the prevalence of fatigue among the sampled population. Statistically significant differences were also found in participants aged 36 to 50, those working shifts, and those working during nighttime hours. Therefore, regulations and practical measures are needed to assess and manage fatigue-related risks, tailored to the aircraft maintenance environment, given that current regulations on human fatigue in aviation primarily focus on flight crew, paving the way for potential changes in the European Aviation Safety Agency regulations.