Avian Influenza Infections In Poultry Research Articles

H7N9 Influenza Virus in China.

In early 2013, human infections caused by a novel H7N9 avian influenza virus (AIV) were first reported in China; these infections caused severe disease and death. The virus was initially low pathogenic to poultry, enabling it to spread widely in different provinces, especially in live poultry markets. Importantly, the H7N9 low pathogenic AIVs (LPAIVs) evolved into highly pathogenic AIVs (HPAIVs) in the beginning of 2017, causing a greater threat to human health and devastating losses to the poultry industry. Fortunately, nationwide vaccination of chickens with an H5/H7 bivalent inactivated avian influenza vaccine since September 2017 has successfully controlled H7N9 avian influenza infections in poultry and, importantly, has also prevented human infections. In this review, we summarize the biological properties of the H7N9 viruses, specifically their genetic evolution, adaptation, pathogenesis, receptor binding, transmission, drug resistance, and antigenic variation, as well as the prevention and control measures. The information obtained from investigating and managing the H7N9 viruses could improve our ability to understand other novel AIVs and formulate effective measures to control their threat to humans and animals.

Avian Influenza A(H5N1) Virus in Egypt.

In Egypt, avian influenza A subtype H5N1 and H9N2 viruses are enzootic in poultry. The control plan devised by veterinary authorities in Egypt to prevent infections in poultry focused mainly on vaccination and ultimately failed. Recently, widespread H5N1 infections in poultry and a substantial increase in the number of human cases of H5N1 infection were observed. We summarize surveillance data from 2009 through 2014 and show that avian influenza viruses are established in poultry in Egypt and are continuously evolving genetically and antigenically. We also discuss the epidemiology of human infection with avian influenza in Egypt and describe how the true burden of disease is underestimated. We discuss the failures of relying on vaccinating poultry as the sole intervention tool. We conclude by highlighting the key components that need to be included in a new strategy to control avian influenza infections in poultry and humans in Egypt.

The Challenge of Controlling Notifiable Avian Influenza by Means of Vaccination

Avian influenza (AI) is an Office International des Epizooties listed disease that has become a disease of great importance both for animal and human health. The increased relevance of AI in animal and human health has highlighted the lack of scientific information on several aspects of the disease, which has hampered the adequate management of some of the recent crises. Millions of animals have died, and there is growing concern over the loss of human lives and over the management of the pandemic potential. The present article reviews the currently available control methods for notifiable AI infections in poultry. The application of control policies, ranging from stamping out to emergency and prophylactic vaccination, is discussed on the basis of data generated in recent outbreaks and in light of new regulations, also in view of the maintenance of animal welfare. Poultry veterinarians working for the industry or for the public sector represent the first line of defense against the pandemic threat and for the prevention and control of this infection in poultry and in wild birds.

Control of avian influenza infections in poultry with emphasis on vaccination

Avian influenza is a World Organization for Animal Heath-listed disease that has become of great importance both for animal and human health. The increased relevance of avian influenza in the fields of animal and human health has highlighted the lack of scientific information on several aspects of the disease, which has hampered the adequate management of some of the recent crises. Millions of animals have died and there is growing concern over the loss of human lives and over the management of the pandemic potential. This special report will review the control methods for avian influenza infections in poultry that are currently available. The application of control policies, ranging from stamping out to emergency and prophylactic vaccination, are discussed on the basis of data generated from recent outbreaks, in the light of new regulations and also in view of the maintenance of animal welfare. Poultry veterinarians working for the industry or for the public sector represent the first line of defense against the pandemic threat and for the prevention and control of this infection in poultry and in wild birds.

Control of avian influenza in poultry.

Avian influenza, listed by the World Organization for Animal Health (OIE), has become a disease of great importance for animal and human health. Several aspects of the disease lack scientific information, which has hampered the management of some recent crises. Millions of animals have died, and concern is growing over the loss of human lives and management of the pandemic potential. On the basis of data generated in recent outbreaks and in light of new OIE regulations and maintenance of animal welfare, we review the available control methods for avian influenza infections in poultry, from stamping out to prevention through emergency and prophylactic vaccination.

Development and validation of an anti-N3 indirect immunofluorescent antibody test to be used as a companion diagnostic test in the framework of a “DIVA” vaccination strategy for avian influenza infections in poultry

Avian influenza (AI) infections have become of growing importance both for animal and human health. Vaccination has become a recommended tool to support eradication efforts and limit the economic losses caused by this disease. The "DIVA" system, using a vaccine containing a heterologous neuraminidase to the field virus, has been shown to be an effective tool in increasing the resistance of birds to field challenge, preventing clinical signs and reducing viral shedding in the environment. The companion diagnostic test to the vaccine, however, has been only partially validated in the field against one subtype of neuraminidase (N1). The present paper presents the results of a full laboratory and field validation of the diagnostic test developed to detect antibodies to the N3 subtype of AI in vaccinated and unvaccinated chickens and turkeys. Antibody kinetic studies conducted in the laboratory have shown that antibodies to the N protein may be detected earlier than antibodies to the haemagglutinin. The data derived from this extensive validation trial indicate the excellent capability of this assay in detecting the presence of active AI infection at an early stage in both unvaccinated and vaccinated birds and the lack of interference with vaccine-induced antibodies.

Lymphocyte - Neutrophil Ratio On Recent Cases of Bird Flu infection in Thailand and Vietnam

Bird flu or avian flu, caused by the H5N1 virus, is an emerging infectious disease. There has been worldwide situation regarding avian influenza infections in poultry since 1997. It is noted that this H5N1 virus jumped the species barrier and caused severe diseases with high mortality in humans in Vietnam and Thailand. Most infected cases usually developed progressive pneumonia with acute respiratory distress syndrome and consequently died. The immunohematological aspect of this viral infection has not been well documented. Here, the author performed this ministudy in order to document the impact of bird flu infection on level of lymphocyte – neutrophil ratio among reported Thai and Vietnamese patients. A literature review regarding human bird flu in Thailand and Vietnam was performed using the database of published work cited in Index Medicus and the Science Citation Index, and also published work from 256 local Thai journals, which are not included in the international citation index. Reports with lack of complete data were excluded from further analysis. According to this review, there were 7 reports [1,2,3,4,5,6, 7] covering 22 Thai and Vietnamese patients with a firm diagnosis of bird flu. The reported lymphocyte – neutrophil ratios ranged from 1:11.5 to 1:0.34 with an average value equals to 1:1.52 ± 1: 1.56 (median = 1:2.2). Of interest, the reported ratios varied, implying variability in cellular response to infection. Generally, viral infection usually induced increased lymphocyte – neutrophil ratios. However, some viral infections such as human immunodeficiency virus (HIV) directly attack the lymphocytes and cause decreased lymphocyte – neutrophil ratios. Concerning the average and mean of reported lymphocyte – neutrophil ratios in bird flu cases, the trend of normal to increased ratio can be seen. CORRESPONDENCE TO Viroj Wiwanitkit, M.D. Department of Laboratory Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok Thailand 10330 Email: wviroj@yahoo.com

Generation of reassortant influenza vaccines by reverse genetics that allows utilization of a DIVA (Differentiating Infected from Vaccinated Animals) strategy for the control of avian influenza

Vaccination of poultry with inactivated influenza vaccine can be an effective tool in the control of avian influenza (AI). One major concern of using inactivated vaccine is vaccine-induced antibody interference with serologic surveillance and epidemiology. In the United States, low pathogenicity H5 and H7 subtype AI viruses have caused serious economic losses in the poultry industry. Most of these viruses also have the accompanying N2 subtype and no H5N1 or H7N8 subtype AI viruses have been identified in poultry in the US. In order to allow the Differentiation of Infected from Vaccinated Animals (DIVA) while maintaining maximum efficacy of the vaccine, we generated reassortant viruses by reverse genetics that contained the same H5 and H7 hemagglutinin (HA) gene as the challenge virus, but a heterologous N1 or N8 neuraminidase (NA) gene. In vaccination-challenge experiments in 2-week-old specific pathogen free chickens, reassortant influenza vaccines (rH5N1 and rH7N8) demonstrated similar antibody profiles and comparable protection rates as vaccines prepared with parent H5N2 and H7N2 viruses. Further, we were able to differentiate the sera from infected and vaccinated birds by neuraminidase inhibition test and indirect immunofluorescent antibody assay on the basis of different antibodies elicited by their NA proteins. These results demonstrate the usefulness of a reverse genetics system for the rapid generation of reassortant AI virus that allows utilization of the DIVA strategy for the control of AI infections in poultry.

Development of a DIVA (Differentiating Infected from Vaccinated Animals) strategy using a vaccine containing a heterologous neuraminidase for the control of avian influenza

The present paper reports of the development and validation of a control strategy for avian influenza infections in poultry. The "DIVA" (Differentiating Infected from Vaccinated Animals) strategy is based on the use of an inactivated oil emulsion vaccine containing the same haemagglutinin (H) subtype as the challenge virus, but a different neuraminidase (N). The possibility of using the heterologous N subtype, to differentiate between vaccinated and naturally infected birds, was investigated through the development of an "ad hoc" serological test based on the detection of specific anti-N1 antibodies. This was achieved using a baculovirus expressing a recombinant N1 protein. The A/ck/Pakistan/H7N3 virus was used as a vaccine and birds were challenged with the HPAI A/ty/Italy/4580/V99/H7N1 strain. The homologous H group ensured a clinical protection of 93% regardless of the vaccination scheme used, and was able to prevent viraemia and muscle colonization in the clinically healthy challenged birds. However, it was not able to prevent viral shedding. The "ad hoc" serological assay was developed as an indirect immunofluorescence test, and was validated using 608 field sera, and showed an "almost perfect agreement" (Kappa value) with the HI test, with relative sensitivity and specificity values of 98.1 and 95.7, respectively. The results of the present investigation suggest that the "DIVA" control strategy may represent a tool for the control of avian influenza infections in poultry.