Average Specific Absorption Rate Research Articles

The effect of exposure to RF-EMF from the laboratory simulator of 5G NR base station on physiological parameters and cognitive abilities of male wistar rats of different ages

In this article, the impact of radiofrequency electromagnetic field (RF-EMF) exposure from a simulated base station for the 5G New Radio (5G NR) telecommunication on rats was studied. The base station affects all age groups of the population, thus, for the first time, the experiment was conducted on male Wistar rats of three different ages (juvenile, adult, and presenile). The base station exposure parameters were chosen according to ICNIRP recommendations for limiting the exposure to radiofrequency electromagnetic field: frequency 2.4 GHz with an average specific absorption rate of 0.0076 W/kg and 0.0059 W/kg over the whole body of experimental animals. Throughout the experiment, body weight was examined weekly, and the dynamics of body weight gain was monitored. Rectal and skin surface temperature on the right hind limb was monitored weekly. Testing in the Morris water maze was performed during the last, Week 5, of RF-EMF exposure. After euthanasia, organ weights were determined in experimental and control animals. None of the investigated parameters did show any statistically significant differences between exposed and control animals of the same age. The data obtained can be used to assess the possible consequences of chronic exposure to RF-EMF from 5G NR base stations.

Design of a Compact Wearable Antenna for IoT Enabled Bio-Telemetry in Diagnostic Health Monitoring System

The structure developed involves the etching of Moore’s curve onto a conventional rectangular patch and subsequently encasing it in a rectangular loop along with a defected ground structure. The proposed antenna exhibits dual-band Since the Internet of Things (IoT) enabled medical diagnostic system requires unprecedented precision and automation for better health care delivery, therefore in such systems, the performance of antennas for efficiently transferring data (vital functions of the human body) is an important task. To address these concerns, a mechanically robust, novel hybrid wearable on-body antenna inspired by Moore’s fractal based geometry and conventional rectangular loop for a diagnostic health monitoring system is proposed. with bandwidth ranging from 1.38 - 1.8 GHz and 2.25 - 4.88 GHz respectively, covering WMTS, ISM, and Personal Communication band besides covering a portion of UWB band also. The two operating bands of the antenna offer a fractional bandwidth of 38.5% and 73.7% respectively. It is observed that the average specific absorption rate (SAR) over the multi-layer phantom is 0.025 W/kg for an input power of ≈ 24 dBm. In comparison to the recently reported wearable antennas, the design proposed has a compact footprint of 0.135 λo x 0.093λo x 0.004λo besides offering a dual- band of operation, peak gain of 2.2 dBi, overall radiated efficiency of 95% and SAR below 0.025 W/kg. The design is fabricated using Rogers 5880 substrate (semi-flex) of thickness 0.75 mm.

Safety Assessment of Electromagnetic Environmental Exposure for GPS Antenna of Electric Vehicle

The present GPS (global positioning system) is widely used in electric vehicle navigation; it is usually installed in the middle-to-rear position on the car’s roof, near the rear windshield. Since the GPS antenna operating frequency is 1.575 GHz, the health risk of the passengers in the car in this high-frequency electromagnetic exposure is a matter of concern. In this paper, we construct models for a GPS antenna, an electric vehicle, and a human body. Through the multiphysics field coupling calculation in COMSOL Multiphysics, a finite element simulation software, in both frequency domain and transient analysis, we obtain the electric field strength distribution, specific absorption rate, and temperature distribution of the human body at three positions inside the electric vehicle after being exposed to the GPS antenna radiation for 30 minutes. The peak human-induced electric field is 18.4 V/m, and the peak specific absorption rate is 0.193 W/kg. The 30 minute average maximum human-induced electric field is 1.6906 V/m, which is 3.1% of the ICNIRP limit, the 30 minute average whole-body SAR is 0.0036 W/kg, which is 4.5% of the ICNIRP limit, and the 30 minute average human core temperature rise is 0.06°C, which is 6% of the ICNIRP limit. In addition, we simulate the impact of three different vehicle shell materials on the level of electromagnetic exposure to the human body, which can provide reference for electromagnetic shielding design in automobiles. The results indicate that the induced electric field strength, specific absorption rate (SAR), and temperature rise in various parts of the human body did not exceed the standard limits in the latest version of ICNIRP. This suggests that the electromagnetic exposure from the GPS antenna in a typical automotive environment does not pose a threat to human health.

Radiofrequency radiation reshapes tumor immune microenvironment into antitumor phenotype in pulmonary metastatic melanoma by inducing active transformation of tumor-infiltrating CD8+ T and NK cells.

Immunosuppression by the tumor microenvironment is a pivotal factor contributing to tumor progression and immunotherapy resistance. Priming the tumor immune microenvironment (TIME) has emerged as a promising strategy for improving the efficacy of cancer immunotherapy. In this study we investigated the effects of noninvasive radiofrequency radiation (RFR) exposure on tumor progression and TIME phenotype, as well as the antitumor potential of PD-1 blockage in a model of pulmonary metastatic melanoma (PMM). Mouse model of PMM was established by tail vein injection of B16F10 cells. From day 3 after injection, the mice were exposed to RFR at an average specific absorption rate of 9.7 W/kg for 1 h per day for 14 days. After RFR exposure, lung tissues were harvested and RNAs were extracted for transcriptome sequencing; PMM-infiltrating immune cells were isolated for single-cell RNA-seq analysis. We showed that RFR exposure significantly impeded PMM progression accompanied by remodeled TIME of PMM via altering the proportion and transcription profile of tumor-infiltrating immune cells. RFR exposure increased the activation and cytotoxicity signatures of tumor-infiltrating CD8+ T cells, particularly in the early activation subset with upregulated genes associated with T cell cytotoxicity. The PD-1 checkpoint pathway was upregulated by RFR exposure in CD8+ T cells. RFR exposure also augmented NK cell subsets with increased cytotoxic characteristics in PMM. RFR exposure enhanced the effector function of tumor-infiltrating CD8+ T cells and NK cells, evidenced by increased expression of cytotoxic molecules. RFR-induced inhibition of PMM growth was mediated by RFR-activated CD8+ T cells and NK cells. We conclude that noninvasive RFR exposure induces antitumor remodeling of the TIME, leading to inhibition of tumor progression, which provides a promising novel strategy for TIME priming and potential combination with cancer immunotherapy.

Design of UWB MIMO antenna using lean wearable textile substrate for reduced SAR

PurposeThis paper aims to propose a two-element dual fed ultra-wideband (UWB) multiple input multiple output (MIMO) antenna system with no additional decoupling structures. The antenna operates from 3.1 to 10.6 GHz. The antenna finds its usage in on-body wearable device applications.Design/methodology/approachThe antenna system measures 63.80 × 29.80 × 0.7 mm. The antenna radiating element is designed by using a modified dumbbell-shaped structure. Jean cloth material is used as substrate. The isolation improvement is achieved through spacing between two elements.FindingsThe proposed antenna has a very low mutual coupling of S21 < −20 dB and impedance matching of S11 < −10 dB. The radiation characteristics are stable in the antenna operating region. It provides as ECC < 0.01, diversity gain >9.9 dB. The antenna offers low average specific absorption rate (SAR) of 0.169 W/kg. The simulated and measured results are found to be in reasonable match.Originality/valueThe MIMO antenna is proposed for on-body communication, hence, a very thin jean cloth material is used as substrate. This negates the necessity of additional material usage in antenna design and the result range indicates good diversity performance and with a low SAR of 0.169 W/kg for on-body performance. This makes it a suitable candidate for textile antenna application.

The impact of variations in subject geometry, respiration and coil repositioning on the specific absorption rate in parallel transmit abdominal imaging at 7 T.

Parallel transmit MRI at 7 T has increasingly been adopted in research projects and provides increased signal-to-noise ratios and novel contrasts. However, the interactions of fields in the body need to be carefully considered to ensure safe scanning. Recent advances in physically flexible body coils have allowed for high-field abdominal imaging, but the effects of increased variability on energy deposition need further exploration. The aim of this study was to assess the impact of subject geometry, respiration phase and coil positioning on the specific absorption rate (SAR). Ten healthy subjects (body mass index[BMI] = 25 ± 5 kg m-2 ) were scanned (at 3 T) during exhale breath-hold and images used to generate body models. Seven of these subjects were also scanned during inhale. Simplifications of the coil and body models were first explored, and then finite-difference time-domain simulations were run with a typical eight-channel parallel transmit coil positioned over the abdomen. Simulations were used to generate 10 g averaged SAR (SAR10g ) maps across 100,000 phase settings, and the worst-case scenario 10 g averaged SAR (wocSAR10g ) was identified using trigonometric maximisation. The average maximum SAR10g across the 10 subjects with 1 W input power per channel was 1.77 W kg-1 . Hotspots were always close to the body surface near the muscle wall boundary. The wocSAR10g across the 10 subjects ranged from 2.3 to 3.2 W kg-1 and was inversely correlated to fat volume percentage (R = 8) and BMI (R = 0.6). The coefficient of variation values in SAR10g due to variations in subject geometry, respiration phase and realistic coil repositioning were 12%, 4% and 12%, respectively. This study found that the variability due to realistic coil repositioning was similar to the variability due to differing healthy subject geometries for abdominal imaging. This is important as it suggests that population-based modelling is likely to be more useful than individual modelling in setting safe thresholds for abdominal imaging.

Effects of Radiofrequency Electromagnetic Field (RF-EMF) exposure on pregnancy and birth outcomes: A systematic review of experimental studies on non-human mammals

Effects of Radiofrequency Electromagnetic Field (RF-EMF) exposure on pregnancy and birth outcomes: A systematic review of experimental studies on non-human mammals

Analyzing the SAR in Human Head Tissues under Different Exposure Scenarios

This paper deals with the assessment of induced specific absorption rate (SAR) in various human models under different exposure scenarios, including both laboratory measurements and simulations. Firstly, SAR values were measured in a standardized SAR laboratory using a phantom for two radiofrequency electromagnetic field (RF-EMF) sources at 900 MHz and 1800 MHz. These laboratory measurements served as a reference for SAR calculations conducted on a specific anthropomorphic mannequin (SAM) using a computer simulation technology (CST) program, thus enabling the determination of antenna location and excitation signal levels for further evaluation. Subsequently, simulations were carried out with CST to evaluate average SAR for the head and for specific head tissues such as the brain, muscles, and fat. Realistic computational human models were also used alongside SAM in CST to explore the influence of gender, age, and tissue type on SAR. Various power levels representing low, moderate, and high RF-EMF exposure were applied to the human models to compare against basic restrictions and reference levels. The simulation results indicate significantly higher SAR values calculated for 1800 MHz compared with 900 MHz. The ratio of the highest SAR values at 1800 MHz to 900 MHz is approximately 1.70 for a baby, 2.59 for a child, and 2.84 for both adult female and adult male. While the SAR values for the brain, fat, muscle, and head are comparable at 900 MHz for the baby, the brain’s SAR value at 1800 MHz stands out significantly from the other tissues. In contrast with the baby, the difference in SAR values between 900 MHz and 1800 MHz is more pronounced for the child, adult female and adult male. The lowest SAR values at 900 MHz and 1800 MHz were obtained for brain tissue in all human models, while the head has the highest SAR value. The maximum SAR change ratio between the brain and the head is calculated to be 4.44 for the male at 1800 MHz. The results reveal that, although the applied electromagnetic field levels were below reference levels for general public local exposure, some local SAR values exceeded the International Commission of Non-Ionizing Radiation Protection’s basic restriction for the general public at certain power levels, particularly at 1800 MHz. The SAR analysis derived from this study is significant in understanding the impact of wireless technologies on health, establishing safety standards, guiding technology advancement, conducting risk assessments, and increasing public awareness.

Biological effects of exposure to 2650 MHz electromagnetic radiation on the behavior, learning, and memory of mice.

With the development of communication technology, the public is paying increasing attention to whether electromagnetic radiation is harmful to health. Mobile phone communication has entered the 5G era, and there are almost no reports on electromagnetic radiation at 2650 MHz. Therefore, it is necessary to evaluate the risk of adverse effects of 5G mobile phone EMR exposure on the human brain. Male animals were continuously exposed to 2650 MHz-EMR for 28 days with a whole-body averaged specific absorption rate (WBSAR) of 2.06 W/kg for 4 h per day. Mouse behavior was assessed using the open-field test (OFT), elevated-plus maze (EPM), and tail suspension test (TST). The Morris water maze (MWM), HE staining, and TUNEL staining were used to evaluate the spatial memory ability and pathological morphology of hippocampal dentate gyrus cells. Additionally, the expression levels of brain-derived neurotrophic factor (BDNF), aminobutyric acid (GABA), and glucocorticoid (GR) in the hippocampus were detected by western blotting and immunohistochemistry, while the corticosterone (CORT) level in serum was detected by ELISA. In the OFT, the total distance traveled, central distance traveled, and residence time significantly decreased in the EMR exposure group (p < .05). In EPM, the percentage of the number of times to open the arm and the percentage of time to open the arm significantly decreased in the EMR exposure group. However, in the TST, the two groups had no significant difference in the 4-min immobility time. In the MWM, the escape latency of the EMR exposure group was shorter than that of the control group, with no significant difference. Furthermore, CORT levels in serum were significantly increased in the EMR exposure group (p < .05), while the expression of BDNF and GR proteins in the hippocampus was reduced (p < .05), but there was no significant difference in GABA expression. Our results indicate that exposure to 2650 MHz-EMR (WBSAR: 2.06 W/kg, 28 days, 4 h per day) had no significant effect on the spatial memory ability of mice (in comparison to little effect). The exposure may be associated with anxiety-like behavior in mice but not related to depression-like behavior in mice.

Design of Low-SAR Terminal Antenna Using Characteristic Mode Manipulation

A novel specific absorption rate (SAR) reduction method based on the characteristic mode theory (CMT) is proposed for mobile terminal. The CMT is introduced to establish the relationship between the characteristic electric field and the induced electric field in human tissues. The design process and mechanism of a low-SAR antenna are demonstrated. To verify the effectiveness of the proposed SAR reduction method, a low-SAR loop antenna is designed. The measurement results show that the 10 g average SAR peak value of the proposed antenna is reduced by 26.1%. The measured −10 dB bandwidth of the low-SAR antenna is 320 MHz, which is increased by 60% compared with the reference antenna (200 MHz). The measured efficiency of the reference antenna and low-SAR antenna are better than 64% and 73% in the entire operating frequency band. With the advantages of bandwidth enhancement, significant SAR reduction and high antenna efficiency, the proposed SAR reduction method is a good choice for the low-SAR terminal antenna design.

SAR Calculation in a Child Seven-Layer Head Model at 2.1 and 2.6 GHz

Health and safety concerns have grown in recent years due to the increasing frequency bands and the demand for wireless communication apparatus. Electromagnetic (EM) radiation breakthrough from Radio frequency (RF) into the human head is an issue that needs to be addressed. Radiation from RF sources can cause serious biological hazards inside the human body. This study measures the average Specific Absorption Rate in a 7-year-old child's head tissues using the ANSYS HFSS software and varying the distance from the source to the antenna in order to address these issues. SAR levels of phones sold should be below certain standard limits. We have used an internal antenna of a mobile phone It's a planar inverted F-antenna (PIFA) with a connected feeding structure.&#x0D;

Whole-Body Exposure System Using Horn Antennas With Dielectric Lens at 28 GHz

In the revised international guidelines and standard for human protection from electromagnetic fields, the permissible specific absorption rate (SAR) for whole-body exposure has been extended from 3 or 10 GHz to 300 GHz. The rationale for this revision is the extrapolation from findings of conventional radio-frequency exposure and infrared radiation. However, experiments of whole-body exposure in human subjects are limited to a few studies, which were conducted below 6 GHz. In this study, an exposure system at 28 GHz targeting a wide area of the human back was developed, which consists of two horn antennas with a dielectric lens. The effectiveness of the proposed exposure system was evaluated by computational dosimetry and experiments. The computed power absorption and measured distributions in the wave absorber and the back of the human subject were in good agreement. Under the compliance with the local exposure limits, the whole-body average SAR was 0.35 W/kg, which is comparable to the limit for whole-body exposure of 0.4 W/kg for occupational exposure in the international guidelines and standard. The developed exposure system would be useful to evaluate the thermophysiological response to whole-body exposure above 6 GHz.

Design of Low-SAR and High On-Body Efficiency Tri-Band Smartwatch Antenna Utilizing the Theory of Characteristic Modes of Composite PEC-Lossy Dielectric Structures

A low specific absorption rate (SAR) and high on-body efficiency tri-band smartwatch antenna is designed utilizing the theory of characteristic modes (TCM) of composite perfect electric conductors and lossy dielectric structures. The antenna works in the GPS frequency band, WLAN 2.4 GHz/Bluetooth frequency band, and 5G frequency band. After analyzing the characteristic modes of the antenna and human body in the above three frequency bands, the characteristic modes with small modal coupling coefficients (MCCs) are selected and excited. Due to the small mutual coupling between the antenna and the human body, the proposed antenna has good performance on both SAR and on-body efficiency. Its measured 10 g average body SAR is lower than 1.3 W/kg. When it is placed 0 mm above the body, its on-body efficiency is 9.3%–9.88% in the GPS frequency band, 12%–14.2% in the WLAN 2.4 GHz/Bluetooth frequency band, and 17.3%–20.1% in the 5G frequency band.

Effect of subject-specific head morphometry on specific absorption rate estimates in parallel-transmit MRI at 7 T.

To assess the accuracy of morphing an established reference electromagnetic head model to a subject-specific morphometry for the estimation of specific absorption rate (SAR) in 7T parallel-transmit (pTx) MRI. Synthetic T1 -weighted MR images were created from three high-resolution open-source electromagnetic head voxel models. The accuracy of morphing a "reference" (multimodal image-based detailed anatomical [MIDA]) electromagnetic model into a different subject's native space (Duke and Ella) was compared. Both linear and nonlinear registration methods were evaluated. Maximum 10-g averaged SAR was estimated for circularly polarized mode and for 5000 random RF shim sets in an eight-channel transmit head coil, and comparison made between the morphed MIDA electromagnetic models and the native Duke and Ella electromagnetic models, respectively. The averaged error in maximum 10-g averaged SAR estimation across pTx MRI shim sets between the MIDA and the Duke target model was reduced from 17.5% with only rigid-body registration, to 11.8% when affine linear registration was used, and further reduced to 10.7% when nonlinear registration was used. The corresponding figures for the Ella model were 16.7%, 11.2%, and 10.1%. We found that morphometry accounts for up to half of the subject-specific differences in pTx SAR. Both linear and nonlinear morphing of an electromagnetic model into a target subject improved SAR agreement by better matching head size, morphometry, and position. However, differences remained, likely arising from details in tissue composition estimation. Thus, the uncertainty of the head morphometry and tissue composition may need to be considered separately to achieve personalized SAR estimation.

Evaluation of Chinese populational exposure to environmental electromagnetic field based on stochastic dosimetry and parametric human modelling.

This study aimed to estimate the distribution of the whole-body averaged specific absorption rate (WBSAR) using several measurable physique parameters for Chinese adult population exposed to environmental electromagnetic fields (EMFs) of current wireless communication frequencies, and to discuss the effects of these physique parameters in the frequency-dependent dosimetric results. The physique distribution of Chinese adults was obtained from the National Physical Fitness and Health Database comprising 81,490 adult samples. The number of physique parameters used to construct the surrogate model was reduced to three via mutual information analysis. A stochastic method with 40 deterministic simulations was used to generate frequency-dependent and gender-specific surrogate models for WBSAR via polynomial chaos expansion. In the simulations, we constructed anatomically correct models conforming to the targeted physique parameters via deformable human modelling technique, which was based on deep learning from the image database including 767 Chinese adults. Thereafter, we analysed the sensitivity of the physique parameters to WBSAR by covariance-based Sobol decomposition. The results indicated that the generated models were consistent with the targeted physique parameters. The estimated dosimetric results were validated using finite-difference time-domain simulations (the error was < 6% across all the investigated frequencies for WBSAR). The novelty of the study included that it demonstrated the feasibility of estimating the individual WBSAR using a limited number of physique parameters with the aid of surrogate modelling. In addition, the population-based distribution of the WBSAR in Chinese adults was firstly presented in the manuscript. The results also indicated that the different combinations of physique parameter, dependent on genders and frequencies, significantly influenced the WBSAR, although the general conservativeness of the guidelines of the International Commission on Non-Ionizing Radiation and Protection can be confirmed in the surveyed population.

Proposed Safety Guidelines for Patient Assistants in an Open MRI Environment.

The wide-open side of an open magnetic resonance imaging (MRI) system allows a patient to easily contact the patient assistant during MRI scans. A wide-open-shaped magnet is highly effective when interventional procedures are necessary. Patient assistants can provide comfort by holding a part of the patient's body. Because current regulations or guidelines are concerned with only patient radio frequency (RF) safety, investigations on the safety of patient assistants exposed to high-magnetic field MRI (up to 1.2 T) are required. In this study, five different poses of patient assistants were numerically simulated at a 1.2 T open MRI system to determine the impact of poses on the RF exposure level. The 10-g averaged specific absorption rate (SAR) levels were analyzed for the poses of each patient assistant wearing gloves. Compared with the patient, up to 29.8% of the patient SAR was observed in the patient assistant. When the patient assistant wore latex gloves, a 63.7% reduction in the 10-g averaged SAR level was observed, which could be a remedy to minimize possible RF hazards. To prevent possible RF hazards during MRI scans, certain clauses regarding the patient assistant's poses or wearing gloves must be added to the existing MRI screening forms.

Acute radiofrequency electromagnetic radiation exposure impairs neurogenesis and causes neuronal DNA damage in the young rat brain

A mobile phone is now a commonly used device for digital media and communication among all age groups. Young adolescents use it for longer durations, which exposes them to radiofrequency electromagnetic radiation (RF-EMR). This exposure can lead to neuropsychiatric changes. The underlying cellular mechanism behind these changes requires detailed investigation. In the present study, we investigated the effect of RF-EMR emitted from mobile phones on young adolescent rat brains. Wistar rats (5 weeks, male) were exposed to RF-EMR signal (2115 MHz) at a head average specific absorption rate (SAR) of 1.51 W/kg continuously for 8 h. Higher level of lipid peroxidation, carbon-centered lipid radicals, and single-strand DNA damage was observed in the brain of rat exposed to RF-EMR. The number of BrdU-positive cells in the dentate gyrus (DG) decreased in RF-EMR-exposed rats, indicating reduced neurogenesis. RF-EMR exposure also induced degenerative changes and neuronal loss in DG neurons but had no effect on the CA3 and CA1 neurons of the hippocampus and cerebral cortex. The activity of Pro-caspase3 did not increase upon exposure in any of the brain regions, pointing out that degeneration observed in the DG region is not dependent on caspase activation. Results indicate that short-term acute exposure to RF-EMR induced the generation of carbon-centered lipid radicals and nuclear DNA damage, both of which likely played a role in the impaired neurogenesis and neuronal degeneration seen in the young brain's hippocampus region. The understanding of RF-EMR-induced alteration in the brain at the cellular level will help develop appropriate interventions for reducing its adverse impact.

Electrically Small Circularly Polarized UWB Intraocular Antenna System for Retinal Prosthesis.

This paper presents the design of an electrically small circularly polarized (CP) 3 × 3 mm2 antenna system as an intraocular unit for retinal prosthesis application. The system is operating in ISM and ultra-wideband (UWB) bands to target high programmability of retina stimulation and recording, respectively. The electrical dimensions, including the ground plane, are λ0/41 × λ0/41 × λ0/191. Physical limitations of the antenna are discussed based on Hansen and Collin's limitations. The proposed wire patch antenna exhibits wideband characteristics by combining multiple modes of the patch antenna in the presence of an interface PCB circuit. By loading polyimide encapsulated patch with stubs, dominant TM010 mode is combined with the higher order modes TM020-TM070 to exhibit wide -10 dB impedance bandwidth of 2-11 GHz. Annular rings and shorting pins in the ground plane provide CP radiation at 2.45, 5.8, and 8 GHz with 3-dB axial-ratio bandwidth of 0.3, 0.16, and 1.2 GHz, and far-field left hand circularly polarized (LHCP) gain of -18.4, -7.6, and -4.7 dBic, respectively, in broadside direction. A biocompatible antenna system is designed using Ansys HFSS in the presence of a detailed multilayer canonical eye model. Additionally, it is examined in an anatomical HFSS head model. Near and far-field electric field distribution is studied along with peak 1-g average specific absorption rate (SAR) calculations. The proposed antenna is fabricated, and the performance, including coupled power from an external antenna, is measured in a custom made eye model including head phantom. A reasonable agreement is obtained between simulated and measured results. To generate an artificial vision, image perception capability could be improved with implantable UWB communication systems that feature particularly high data-rate and small size.

Effect of radiofrequency exposure on body temperature: Real-time monitoring in normal rats

Radiofrequency radiation (RFR) can generate heat in living organisms. In this study, we monitored the body temperature of healthy animals during RFR exposure in real time using an implantable iButton data logger. A reverberation chamber system for small animals was used for this radiofrequency (RF) exposure in vivo study. Healthy male Sprague-Dawley rats were divided into two groups: with versus without iButton implantation (n = 20 per group). Each group was further divided into a sham-exposed and RF-exposed group (n = 10 per subgroup). Rats were exposed to a 1,760-MHz long-term evolution (LTE) signal in the reverberation chamber system at a whole-body average specific absorption rate of 0 W/kg (sham-exposed) or 4 W/kg (RF-exposed) for 6 h. The body temperature of iButton-implanted rats was recorded using an intraperitoneally implanted iButton every minute over 6 h of RF exposure, whereas that of non-implanted rats was measured directly using a rectal thermometer immediately before and after the 6-h RF exposure period. The temperature values measured by the two types of thermometers were significantly positively correlated (r = 0.63, P < 0.01, linear regression), and changes in body temperatures recorded in iButton-implanted and non-implanted rats measured using two thermometers after 6 h of RF exposure were maintained within <1°C (P = 0.87, general linear model, followed by univariate model). Similar results were obtained for rectal thermometer measurements (P = 0.12, paired t-test). These results suggest that RF exposure at a whole-body average specific absorption rate of 4 W/kg does not induce significant changes in body temperature in healthy rats over a 6-h RF exposure period.

Investigation of oxidative damage, antioxidant balance, DNA repair genes, and apoptosis due to radiofrequency-induced adaptive response in mice

ABSTRACT This study aims to determine whether exposure to non-ionizing radiofrequency fields could induce an adaptive response (AR) in adult mice and to reveal potential molecular mechanisms triggered by RF-induced AR. The study was performed on 24 adult male Swiss-Albino mice. The average mass of the mice was 37 g. Four groups of adult mice, each consisting of 6, were formed. The radiofrequency group (R) and the adaptive response group (RB) were exposed to 900 MHz of global system for mobile communications (GSM) signal at 0.339 W/kg (1 g average specific absorption rate) 4 h/day for 7 days, while the control group (C) and the bleomycin group (B) were not exposed. 20 minutes after the last radiofrequency field (RF) exposure, the mice in the B and RB groups were injected intraperitoneal (ip) bleomycin (BLM), 37.5 mg/kg. All the animals were sacrificed 30 minutes after the BLM injection. Oxidative damage and antioxidant mechanism were subsequently investigated in the blood samples. Changes in the expression of the genes involved in DNA repair were detected in the liver tissue. TUNEL method was used to determine the apoptosis developed by DNA fragmentation in the liver tissue. The RB group, which produced an adaptive response, was compared with the control group. According to the results, the increase of reactive oxygen species (ROS) in the RB group may have played an important role in triggering the adaptive response and producing the required minimum stress level. Furthermore, tumor suppressor 53(p53), oxo guanine DNA glycosylase (OGG-1) levels responsible for DNA repair mechanism genes expression were increased in conjunction with the increase in ROS. The change in the poly (ADP-ribose) polymerase 1 (PARP-1) and glutathione peroxidase 1 (GPx-1) gene expression were not statistically significant. The antioxidant enzyme levels of superoxide dismutase (SOD), catalase (CAT), and total antioxidant capacity (TAC) were decreased in the group with adaptive response. According to the data obtained from terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) analysis, apoptosis was decreased in the RB group due to the decrease in cell death, which might have resulted from an increase in gene expression responsible for DNA repair mechanisms. The results of our study show that exposure to RF radiation may create a protective reaction against the bleomycin. The minimal oxidative stress due to the RF exposure leads to an adaptive response in the genes that play a role in the DNA repair mechanism and enzymes, enabling the survival of the cell.