Average Daily Dietary Intake Research Articles

An error in concept and definition of recommended dietary allowance developed in 1941: Time to create new ones

The recommended dietary allowance (RDA) for vitamin C differs greatly (40–110 mg/d) across countries. In addition, vitamin D deficiency is, effectively, a pandemic, with a prevalence of approximately 25% to 80% across countries according to the findings in the recent scientific literature. However, these phenomena are attributable to a serious error in the concept and definition of RDA, which was developed in 1941. The current definition of RDA is the average daily dietary nutrient intake level that is deemed sufficient to meet the nutrient requirements of almost all (97–98%) healthy individuals. This is an extremely high intake level for a specific nutrient affecting only a few individuals (the upper 2.5%) of the generally healthy population. The RDA should be redefined by investigating at which intake level of a specific nutrient the incidence of a specific disease or mortality increases or decreases based on prospective cohort studies.

Abstract 2212: Global cancers attributable to modifiable risk factors: Dietary factors of animal origin

Abstract Cancer is the leading cause of death worldwide. Modifiable risk factors including diet have been linked to a wide range of malignancies. We estimated the global cancer population-attributable fraction (PAF) to established and suspected dietary cancer risk factors according to the Human Development Index (HDI).Diet-cancer associations were selected from WCRF, GBD and IARC report. Dietary factors of animal origin include red meat, processed meat, fish and dairy products; cancers include nasopharynx, esophageal, stomach, colorectal, pancreatic, lung, prostate, breast cancer. Gender-specific PAF were calculated based on 1) the total and gender-specific 2022 country-specific population from World Bank; 2) the country-specific average daily dietary factors intake (g/day) for 2000-2005 from the Global Dietary Database; 3) relative risks from the meta-analyses and WCRF reports; 4) country-specific 2020 cancer incidence from WHO. Estimates were also stratified by Human Development Index (HDI).Table 1 provides the results. The PAF for red meat and processed meat was higher in high HDI countries, while that for low dairy product intake was higher in low HDI countries. According to results, changes in selected dietary factors can prevent cancer-related incidence, particularly depending on where development status of countries and access to the source of their intake. Table1: Population attributable fractions for incidence of cancer in 2020 by gender. Dietary factors and Related Cancers Relative risk (g/day) AF (N. global) – Both gender AF (N. global)- Female AF (N. global)- Male Red meat Nasopharynx(L) 1.35(100) 0.103(13750.2) 0.102(3734.9) 0.104(10015.3) Colorectal (S) 1.12(100) 0.040(78714.5) 0.040(33981.2) 0.041(44733.2) Pancreatic (L) 1.11(100) 0.037(27420.3) 0.036(8479.8) 0.037(18940.4) Lung (L) 1.22(100) 0.070(155986.9) 0.069(52442.6) 0.070(103544.4) Prostate(L) 1.04(100) 0.014(20665.6) - 0.014(20665.6) Effect on total cancers * 0.016(296537.5) 0.011(98638.5) 0.021 (197898.9) Processed meat Nasopharynx(L) 1.46(30) 0.164(22194.6) 0.154(5637.4) 0.173(16557.1) Esophagus(L) 1.3(100) 0.036(22654.9) 0.034(6345.8) 0.038(16309.2) Stomach(L) 1.21(50) 0.052(60325.8) 0.049(18161.7) 0.055(42164) Colorectal (S) 1.18(100) 0.023(45460.9) 0.021(18522.8) 0.024(26938.1) Pancreatic(L) 1.17(50) 0.043(21900.2) 0.040(9414.4) 0.046(12485.8) Lung (L) 1.14(50) 0.036(82762.9) 0.034(26023.9) 0.038(56739) Effect on total cancers * 0.014(255299.3) 0.010(84106) 0.018(171193.2) Dairy products Colorectal(S) 0.87(400) 0.092(180063.4) 0.091(77598.8) 0.093(102464.6) Breast (L) 0.95(200) 0.068(151960.9) 0.068(151960.9) - Prostate (L) 1.07(400) 0.016(24224.3) - 0.016(24224.3) Effect on total cancers * 0.019(356248.6) 0.026(229559.7) 0.013(126688.9) Fish Colorectal (L) 0.89(100) 0.022(44882.9) 0.022(18705.9) 0.024(26177) Effect on total cancers * 0.002(44882.9) 0.002(18705.9 0.003(26177) Mean (g/day) of global intake Processed meat (B=14.2/M=15.1/F=13.3), red meat (B=36.5/M=36.9/F=36), fish(B=23/M=22.1/F=23.8), dairy products(B=77/M=74.9/F=79)S=strong evidenceL=limited evidence*All cancers excl. non-melanoma skin cancer (B= 18094716/F=8751759/M=9342957) 1 Citation Format: Monireh Sadat Seyyedsalehi, Edward Giovannucci, Paolo Boffetta. Global cancers attributable to modifiable risk factors: Dietary factors of animal origin [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 2212.

Epigenetic aging in older people living with HIV in Eswatini: a pilot study of HIV and lifestyle factors and epigenetic aging

BackgroundPeople living with HIV (PLHIV) on effective antiretroviral therapy are living near-normal lives. Although they are less susceptible to AIDS-related complications, they remain highly vulnerable to non-communicable diseases. In this exploratory study of older PLHIV (OPLHIV) in Eswatini, we investigated whether epigenetic aging (i.e., the residual between regressing epigenetic age on chronological age) was associated with HIV-related parameters, and whether lifestyle factors modified these relationships. We calculated epigenetic aging focusing on the Horvath, Hannum, PhenoAge and GrimAge epigenetic clocks, and a pace of biological aging biomarker (DunedinPACE) among 44 OPLHIV in Eswatini.ResultsAge at HIV diagnosis was associated with Hannum epigenetic age acceleration (EAA) (β-coefficient [95% Confidence Interval]; 0.53 [0.05, 1.00], p = 0.03) and longer duration since HIV diagnosis was associated with slower Hannum EAA (− 0.53 [− 1.00, − 0.05], p = 0.03). The average daily dietary intake of fruits and vegetables was associated with DunedinPACE (0.12 [0.03, 0.22], p = 0.01). The associations of Hannum EAA with the age at HIV diagnosis and duration of time since HIV diagnosis were attenuated when the average daily intake of fruits and vegetables or physical activity were included in our models. Diet and self-perceived quality of life measures modified the relationship between CD4+ T cell counts at participant enrollment and Hannum EAA.ConclusionsEpigenetic age is more advanced in OPLHIV in Eswatini in those diagnosed with HIV at an older age and slowed in those who have lived for a longer time with diagnosed HIV. Lifestyle and quality of life factors may differentially affect epigenetic aging in OPLHIV. To our knowledge, this is the first study to assess epigenetic aging in OPLHIV in Eswatini and one of the few in sub-Saharan Africa.

Dietary Magnesium Replacement for Prevention of Hypomagnesemia in Patients With Ovarian Cancer Receiving Carboplatin-Based Chemotherapy.

Hypomagnesemia is a common side effect of platinum-based chemotherapy and predicts poor overall survival in some cancers. Standard magnesium replacement strategies are often inadequate for maintaining magnesium levels. We hypothesized that a daily dietary magnesium replacement approach through magnesium-rich foods would help maintain adequate magnesium levels during platinum-based treatment. We conducted a prospective feasibility study of magnesium-rich diets in patients 18 years and older with previously untreated ovarian cancer scheduled to receive carboplatin-containing chemotherapy of at least six consecutive cycles. Education about magnesium-rich diets was provided at enrollment and then weekly during chemotherapy. Feasibility was defined as ≥60% completion of dietary recalls and ≥280 mg average daily dietary magnesium intake across all patients. Twenty-one of 26 patients enrolled completed at least five chemotherapy cycles and were included in the analysis. Adherence to the study diet was 76%. Daily dietary magnesium intake was 100.5 mg at baseline and increased throughout each cycle: 6% of patients at baseline, 24% after the first cycle, and 67% after the fifth cycle reached ≥280-mg/day magnesium intake. Seven (33%) of 21 had at least one incident of hypomagnesemia. Patients who were adherent had significantly lower incidence of hypomagnesemia (19% v 80%, P = .03) and less need for intravenous magnesium (6% v 60%, P = .03) than those who were nonadherent. The study achieved primary feasibility objectives of retention and adherence to the study intervention. Weekly education about magnesium-rich diets was effective in increasing dietary magnesium intake. Adequate dietary magnesium appeared to be protective against hypomagnesemia.

Flavan-3-ol monomers intake is associated with osteoarthritis risk in Americans over 40 years of age: results from the National Health and Nutritional Examination Survey database.

Objectives: Osteoarthritis (OA) stands as the prevailing progressive musculoskeletal disease, serving as the primary cause of chronic pain and activity limitations among adults over 40. Flavan-3-ols, common polyphenolic compounds, are believed to harbor anti-inflammatory and anti-aging properties. This study explores the relationship between flavan-3-ol intake and osteoarthritis risk in individuals over the age of 40 in the US. Methods: This study included 7452 participants over the age of 40 from three cycles (2007-2008, 2009-2010, and 2017-2018) of the National Health and Nutrition Examination Survey. Information on OA history was obtained via home surveys. Information on flavan-3-ol monomers intake was obtained using a survey from the Food and Nutrient Database for Dietary Studies. We used a logistic regression model and restricted cubic spline to analyze the relationships between flavan-3-ol monomers and OA. Stratified analyses were also conducted in this study. Results: There were 1056 participants with OA and 6396 without OA. Compared to the first tertile (T1) group, the adjusted odds ratio with a 95% confidence interval (CI) of logistic regression model 2 for the flavan-3-ol T2 group was 1.296 (0.979-1.715) (p = 0.068), the OR for (-)-epigallocatechin was 1.292 (1.025-1.629) (p = 0.032), and the OR for (-)-epicatechin 3-gallate was 1.348 (1.013, 1.793) (p = 0.042). A dose-response curve indicated a non-linear association (p for non-linearity <0.05) between OA and total flavan-3-ol monomers (nadir point: 483.29 mg, 95% CI: 0.61-0.90). No interaction effects were found in the subgroup analysis. Conclusions: In individuals over 40 in the US, the average daily dietary intake of flavan-3-ol monomers manifests a J-shaped relationship with OA risk.

Dietary Magnesium Intake and Proteinuria: Is There a Relationship?

The possible relationship between dietary magnesium status and proteinuria has been suggested by a number of previous studies. However, human studies on this association are limited. Therefore, the present study aimed to investigate the independent relationship between dietary magnesium intake and urinary protein excretion. The present study was a post hoc analysis of the previous randomized clinical trial that evaluated the effect of dietary phosphorus restriction on proteinuria. The baseline data of 90 participants with proteinuria and chronic kidney disease was used to measure the association between dietary magnesium intake and proteinuria. Participants were asked to record their 24-h food intake for three days a week in a questionnaire. Urinary protein to creatinine ratio (UPCR) in a random urine sample was measured to be a marker for proteinuria. Out of 90 patients included in the study, 47 were men and 43 were women. The mean ± standard deviation of age and body mass index were 59.05 ± 14.16 years and 29.02 ± 5.54 kg/m2, respectively. The patients' average daily dietary intake of energy and magnesium were 2183 kcal and 169.44 mg, respectively. A significant inverse correlation was found between the dietary intake of magnesium and UPCR (r = - 0.219, p = 0.042). This association remained significant even after adjusting for confounding variables (β = - 0.222, p = 0.028). The findings of the present study showed a significant inverse relationship between the magnesium intake and proteinuria. Although, the design of the current research was cross-sectional, it has provided a basis for conducting future longitudinal studies and trials to better elucidate such a relationship.

Effects of Zinc (Zn) on the Growth of the Progeny from the Formation of Foetal Sperm through Parturition: A Review

The amount of zinc (Zn), another extremely important trace element, in human beings is substantial. Since the human body is unable to retain zinc, dietary intake of zinc is necessary for a number of bodily processes and metabolism. The testicles, epididymis, and prostate are the three main accessory sex glands, and normal development of these glands depends on the intake of Zn as it moves through the body. The early phases of the growth of germ cells and sperm development, the stages of sperm cell maturation and development, ejaculating the liquefaction the attachment of the male reproductive system and prostasomes, capacity, and fertilization are all important processes in which it participates. During ejaculation, the male reproductive system discharges higher zinc into the seminal fluid, which is important for the release and motility of sperm. The portion of Zn is essential during the gestational, labour, perinatal, and neonatal periods. During the maternity period, the average daily dietary intake of zinc in developing nations is 8–12 mg. Critical evidence has been used to discuss the lack of Zn and its effects. A thorough summary of the activities and roles played by Zn in effective fertilization has been provided. In the simplest terms, our current research highlights Zn's importance at every step of human reproduction, from spermatogenesis through birthing. Future research on reproductive biology now has a new avenue to explore thanks to the significance of zinc and its supplementation in in-vitro fertilization (IVF).

Association between dietary intake of acrylamide and increased risk of mortality in women: Evidence from the E3N prospective cohort

Acrylamide is an organic compound classified as probably carcinogenic to humans because of sufficient evidence in animals but not in humans. Other health risks associated with acrylamide intake are still not fully elucidated. We aimed to study the relationship between acrylamide dietary intake and mortality in the E3N (Etude Epidémiologique auprès de femmes de l'Education Nationale) French cohort.We studied 72,585 women of the E3N prospective cohort, which completed a food frequency questionnaire in 1993. The E3N food consumption database and the food contamination database obtained from the second French total diet study were used to estimate participants' average daily acrylamide dietary intake. We estimated the associations between acrylamide dietary intake and all-cause or cause-specific mortality using Cox proportional hazard models.During follow-up (1993–2014), we identified 6441 deaths. The mean acrylamide dietary intake was 32.6 μg/day, with coffee consumption as principal contributor (48.6 %). In the fully adjusted model, we found a non-linear association between acrylamide dietary intake and all-cause mortality and a linear positive association with cardiovascular disease (HR per one STD increment [95%CI]: 1.11 [1.02; 1.21]), all-cancer (HR [95%CI]: 1.05 [1.01; 1.10]) and lung cancer (HR [95%CI]: 1.22 [1.09; 1.38]) mortality, while we observed no association with breast (HR [95%CI]: 0.94 [0.86; 1.03]) and colorectal (HR [95%CI]: 1.12 [0.97; 1.29]) cancer mortality. We highlighted an interaction between acrylamide dietary intake and smoking status in the models for all-cause and all-cancer mortality: when stratifying on smoking status, statistically significant positive associations were observed only in current smokers.This study on a large prospective cohort following more than 70,000 women for over 20 years suggests that higher acrylamide dietary intakes are associated with an increased risk of mortality. Therefore, it is essential to keep reducing acrylamide contamination and prevent dietary intake of acrylamide, especially among smokers.

Integration of multiomics with precision nutrition for gestational diabetes: Study protocol for the Westlake Precision Birth Cohort

Integration of multiomics with precision nutrition for gestational diabetes: Study protocol for the Westlake Precision Birth Cohort

Choline Intake and Cognitive Function Among U.S. Older Adults

Choline is an essential nutrient affects brain development in early life. However, evidence is lacking regarding its potential neuroprotective effects in later life from community-based cohorts. This study assessed the relationship between choline intake and cognitive functioning in a sample of older adults 60 years + from the National Health and Nutrition Examination Survey 2011–2012 and 2013–2014 waves (n = 2,796). Choline intake was assessed using two nonconsecutive 24-hour dietary recalls. Cognitive assessments included immediate and delayed word recalls, Animal Fluency, and Digit Symbol Substitution Test. The average daily dietary choline intake was 307.5 mg, and the total intake (including intake from dietary supplements) was 330.9 mg, both below the Adequate Intake level. Neither dietary OR = 0.94, 95% CI (0.75, 1.17) nor total choline intake OR = 0.87, 95% CI (0.70, 1.09) was associated with changes in cognitive test scores. Further investigation adopting longitudinal or experimental designs may shed light on the issue.

Analysis of differentiated regional dietary patterns of adults aged 18-64 years in 15 provinces( autonomous regions, municipalities) in 2018

To analyze the dietary patterns of Chinese adults in 15 provinces and cities in China. A total of 9208 adults aged 18-64 from the "China Health and Nutrition Survey" in 2018 were selected as subjects. Food consumption data were collected through 3 d-24 h dietary recalls and weighing household cooking oil and condiments, and the average daily dietary nutrient intake was calculated by the food composition table. Clustering analysis was used to analyze dietary patterns in different regions, and data from the 2009 China Health and Nutrition Survey were used for comparison. Three dietary patterns were obtained in this study, including northern pattern, coastal pattern and southern pattern. The analysis of demographic characteristics showed that the intake of cereals, vegetables and livestock were statistically different among different age groups, and livestock and poultry intake was higher in the 18-49 years; Fruit and milk were higher in women and other foods were higher in men; The intakes of cereals, tubers, legumes and vegetables were higher in rural areas, people with low income and low education levels, and other foods were higher in urban areas, people with high income and high education levels, especially milk. Compared with the dietary guidelines, it was found that the proportion of insufficient intake of dark vegetables, fruits and nuts in three patterns and milk, eggs in the southern pattern and aquatic products in the northern pattern was up to 80%, the other food intake was in 50%-80%; The proportion of excessive intake of livestock and poultry meat in the southern mode was significantly higher, reaching 78%. Nutrient analysis showed that the intake of carbohydrate, dietary fiber and carbohydrate energy ratio, vitamin E, magnesium and manganese were higher in the northern pattern; The intakes of protein, protein energy ratio, riboflavin, potassium, sodium, calcium, phosphorus, copper and selenium were higher in the coastal pattern; The southernpattern had a higher intake of energy, fat, fat energy ratio, niacin, retinol, zinc and vitamin C. In addition, the comparison between 2009 and 2018 showed that the intake of most foods except milk has decreased to varying degrees in the past decade, while the intake of some foods has increased. The 15 provinces in China were divided into three dietary patterns. Unbalanced intake of food and nutrients was common among them, and the intake was influenced by different demographic characteristics. In addition, most food intake has declined over the past decade.

Indicators of oxidative stress in leukocytes isolated from bottlenose dolphins (Tursiops truncatus) in response to a proinflammatory challenge

Few studies have analyzed the indicators of oxidative stress in marine mammals following exposure to lipopolysaccharides (LPS); sex and maturity-related differences have not been explored. The objective of this study was to compare the indicators of oxidative stress following exposure to LPS for 24 and 48 h in isolated Pacific bottlenose dolphin (Tursiops truncatus; N = 12) leukocytes in relation to sex and maturity stage, using spectrophotometry. Following 48 h under experimental conditions (10 μg LPS mL−1), the leukocytes from males (n = 5) produced significantly more superoxide radical (O2•-; F (1, 8) = 13.965, p = 0.006) and displayed significantly greater activities of catalase (CAT; F (1, 8) = 9.465, p = 0.015) and glutathione S-transferase (GST; p = 0.028) compared to the leukocytes from females (n = 7). Following 48 h under experimental conditions, maturity-stage did not significantly influence the indicators of oxidative stress. Mature bottlenose dolphins (n = 7) had a significantly higher average daily dietary intake compared to immature bottlenose dolphins (n = 5; F (1, 10) = 5.825, p = 0.036). These results suggest that sex-related strategies for coping with a proinflammatory challenge may be present within the leukocytes from bottlenose dolphins, while potential maturity stage-related strategies require further investigation.

Human exposure to phthalate esters in soils embodied in interregional food trade in China

The ubiquitous occurrence of phthalate esters (PAEs) in agricultural soil results in their inevitable accumulation in crops, potentially increasing the risk of human exposure to PAEs via daily food intake. Dietary health risk of PAEs not only depends on locally produced food but also the imported food from other regions. However, the impact of interregional food trade on human dietary exposure to PAEs has been seldom assessed. Herein, we investigated the impact of interregional food trade on the dietary exposure to PAEs that contributed from soil contamination in China. The average daily dietary intake of PAEs for the Chinese general population was 24.3 μg/kg/day when assuming the total consumption of crops from local market only, while the average daily dietary intake of PAEs for the Chinese general population was decreased by 2.9% when the effects of interregional food trade were involved into the calculation. Additionally, the interregional food trade remarkably increased the daily dietary intake of PAEs in the regions of Beijing-Tianjin region (47.8%), North (21.4%) and Central (4.26%). As a result, the hazard quotient value of PAEs in the regions of Beijing-Tianjin region, North and Central increased by 29.4%, 11.0% and 5.0%, respectively, owing to the consumption of imported crops from the highly PAEs contaminated regions. In contrast, the daily intake and hazard quotient value of PAEs in the regions of Central Coast, Northwest, Northeast and South Coast decreased due to the interregional trade. These results indicated that the interregional food trade promoted the transfer of PAEs between regions and thus altered the potential risk to the local population. Overall, this study highlights the importance of taking the interregional food trade into account to provide a more accurate risk assessment of dietary exposure to pollutants.

Gestational weight gain and dietary energy, iron, and choline intake predict severity of fetal alcohol growth restriction in a prospective birth cohort

Animal models have demonstrated that maternal nutrition can alter fetal vulnerability to prenatal alcohol exposure (PAE). Few human studies have examined the role of nutrition in fetal alcohol spectrum disorders (FASD). Our objectives were to examine whether fetal vulnerability to PAE-related growth restriction is modified by: 1) rate of gestational weight gain; or prenatal dietary intakes of 2) energy, 3) iron, or 4) choline. In a prospective longitudinal birth cohort in Cape Town, South Africa, 118 heavy-drinking and 71 abstaining/light-drinking pregnant women were weighed and interviewed regarding demographics, alcohol, cigarette/other drug use, and diet at prenatal visits. Infant length, weight, and head circumference were measured at 2 wk and 12 mo postpartum. Heavy-drinking mothers reported a binge pattern of drinking [Mean = 129mL (∼7.2 drinks)/occasion on 1.3 d/wk). Rate of gestational weight gain and average daily dietary energy, iron, and choline intakes were similar between heavy-drinking women and controls. In regression models adjusting for maternal age, socioeconomic status, cigarette use, and weeks gestation at delivery, PAE [ounces (30mL) absolute alcohol per day] was related to smaller 2-wk length and head circumference and 12-mo length, weight, and head circumference z-scores (β = -0.43 to -0.67; all P values<0.05). In stratified analyses for each maternal nutritional measure (inadequate compared with adequate weight gain; tertiles for dietary energy, iron, and choline intakes), PAE-related growth restriction was more severe in women with poorer nutrition, with effect modification seen by weight gain, energy, iron, and/or choline for several anthropometric outcomes. Gestational weight gain and dietary intakes of energy, choline, and iron appeared to modify fetal vulnerability to PAE-related growth restriction. These findings suggest a need for screening programs for pregnant women at higher risk of having a child with FASD to identify alcohol-using women who could benefit from nutritional interventions.

Iron Absorption: Factors, Limitations, and Improvement Methods

Iron is an essential element for human life since it participates in many functions in the human body, including oxygen transport, immunity, cell division and differentiation, and energy metabolism. Iron homeostasis is mainly controlled by intestinal absorption because iron does not have active excretory mechanisms for humans. Thus, efficient intestinal iron bioavailability is essential to reduce the risk of iron deficiency anemia. There are two forms of iron, heme and nonheme, found in foods. The average daily dietary iron intake is 10 to 15 mg in humans since only 1 to 2 mg is absorbed through the intestinal system. Nutrient–nutrient interactions may play a role in dietary intestinal iron absorption. Dietary inhibitors such as calcium, phytates, polyphenols and enhancers such as ascorbic acid and proteins mainly influence iron bioavailability. Numerous studies have been carried out for years to enhance iron bioavailability and combat iron deficiency. In addition to traditional methods, innovative techniques are being developed day by day to enhance iron bioavailability. This review will provide information about iron bioavailability, factors affecting absorption, iron deficiency, and recent studies on improving iron bioavailability.

Dietary Micronutrient Status and Relation between Micronutrient Intakes and Overweight and Obesity among Non-Pregnant and Non-Lactating Women Aged 18 to 49 in China.

Women between the ages of 18 and 49 are women of reproductive age, for whom physical health and nutritional status are closely related to successful pregnancy, good pregnancy outcomes and the nurturing of the next generation. Overweight and obesity have become important nutrition and health problems of women aged 18–49 years in China. In social life, non-pregnant and non-lactating Chinese women aged 18–49 are the most vulnerable and neglected group. At present, there are no research data on their dietary micronutrient intake, and the relationship between dietary micronutrient intake and overweight and obesity in China. However, non-pregnant and non-lactating women aged 18–49 are the best window of opportunity to implement strategies, correct nutrition and improve physical health. It remains to be explored whether their overweight and obesity are related to inadequate dietary micronutrient intake. The aim of this study was to evaluate dietary micronutrient intake in non-pregnant and non-lactating Chinese women aged 18–49 years, and to analyze the relationship between dietary micronutrient intake and overweight and obesity. Data were obtained from 2015 China Adult Chronic Disease and Nutrition Surveillance (CACDNS 2015). In CACDNS 2015, 12,872 women aged 18 to 49 years (excluding pregnant women and lactating mothers) were surveyed for a three-day 24 h dietary recall and a three-day household weighing of edible oil and condiments. The average daily dietary intake of micronutrients was calculated according to the Chinese food composition table. In 2015, the median intake of vitamin A, vitamin B1, vitamin B2, vitamin C and folate in non-pregnant and non-lactating women aged 18–49 years in China was 267.0 μg RE/day, 0.7 mg/day, 0.6 mg/day, 63.5 mg/day and 121.0 μg/day, respectively. The median mean intake of vitamin A, niacin, calcium and zinc in overweight/obese group was lower than that in non-overweight/obese group, and the difference was statistically significant (p < 0.05). Multivariate Logistic regression analysis showed that vitamin A intake (Q3 vs. Q1: OR = 0.785, 95% CI: 0.702~0.878; Q4 vs. Q1: OR = 0.766, 95% CI: 0.679~0.865), niacin intake (Q2 vs. Q1: OR = 0.801, 95% CI: 0.715–0.898; Q3 vs. Q1: OR = 0.632, 95% CI: 0.554~0.721; Q4 vs. Q1: OR = 0.662, 95% CI: 0.568~0.772), Zinc intake (Q4 vs. Q1: OR = 0.786, 95% CI: 0.662~0.932) were a protective factor for overweight/obesity in women, while vitamin B2 intake (Q2 vs. Q1: OR = 1.256, 95% CI: 1.120~1.408; Q3 vs. Q1: OR = 1.416, 95% CI: 1.240~1.617; Q4 vs. Q1: OR = 1.515, 95% CI: 1.293–1.776), vitamin E intake (Q2 vs. Q1: OR = 1.114, 95% CI: 1.006–0.235; Q3 vs. Q1: OR = 1.162, 95% CI: 1.048~0.288; Q4 vs. Q1: OR = 1.234, 95% CI: 1.112–1.371) was a risk factor for overweight/obesity in females. The intakes of most dietary micronutrients in non-pregnant and non-lactating women aged 18–49 in China were low. The intakes of dietary vitamin A, niacin and zinc were negatively correlated with the risk of overweight/obesity, while the intakes of vitamin B2 and vitamin E were positively correlated with the risk of overweight/obesity.

Food restriction protects the myenteric nervous population of rats with type 2 diabetes mellitus / A restrição alimentar protege a população nervosa mioentérica de ratos com diabetes mellitus tipo 2

Background: Type 2 diabetes affects the intestine. Food restriction (FR) promotes benefits, but the studies of its effects in rats are scarce. This study aims to analyze the influence of two type 2 diabetes models and FR over the intestine morphology. Methods: Thirty Wistar rats formed three groups (n=10/group) treated for two months: C (control); DE (diabetic with streptozotocin+cafeteria-style diet); and DN (diabetic with streptozotocin+nicotinamide). These groups were subdivided into six during two months: CC (control), CCR (control+food restriction), DEC (diabetic+standard diet), DER (diabetic+food restriction), DNC (diabetic+standard diet) and DNR (diabetic+food restriction). FR was 50% of the average daily dietary intake of group C. Jejunum and ileum samples were collected for evaluation of wall morphometry, goblet cell number, number and profile of myenteric neurons and glia. Results: The DE model promoted wall reduction in both segments, while DN jejunum was increased. Goblet cells were reduced in the jejunum for both diabetic models and FR. Both intestinal segments presented reduction of neuronal and glial myenteric populations in diabetes. Conclusions: FR promoted protection of myenteric neurons and glial cells against diabetic damage. The jejunum and ileum respond differently to diabetes and FR. FR has positive effects over the small intestine, mainly over the enteric nervous system.

Lead contamination status and assessment of potential risk to human health of commercial foods in Guangzhou City in 2017-2019

To investigate lead contamination in commercial foods in Guangzhou City, and to assess the health risk of dietary lead exposure in the residents. Food samples were collected in 11 districts in Guangzhou City from 2017 to 2019.The samples were tested for lead by using inductively coupled plasma mass spectrometry. The dietary exposure to lead of residents of different age groups in Guangzhou was calculated in combination with the intake of foods from the food consumption survey of Guangzhou residents in 2011, and the margin of exposure(MOE) method was used to evaluate the health risk of the population. A todal of 3130 food samples of 15 categories were collected. The overall lead detection rate was 82.11%, the exceeding rate was 0.13%, and the average lead content was 0.031 mg/kg. The highest lead levels were found in bivalve, edible fungi and flour and flour products. Excessive levels of lead were found in bivalve, eggs and egg products, and flour and flour products. The average daily dietary intake of lead in people aged 3-6, 7-17, 18-59 and over 60 in Guangzhou were 0.368, 0.377, 0.326 and 0.337 μg/kg, respectively. Daily lead exposure of population with high consumption(P95) in various age groups through foods were 1.091, 1.079, 0.906 and 0.925 μg/kg, respectively. Brassica and leafy vegetables, rice and its products and meat were the main sources of dietary lead exposure, accounting for 53.28% of the total dietary lead exposure. The average MOE of lead in all age groups were greater than 1, but the P95 of MOE value of people aged 3-6 and 7-17 were 0.55 and 0.56, respectively, which presented certain health risks. There was a certain amount of lead exceeding the standard in commercial foods in Guangzhou. The average level of dietary lead exposure for residents of all ages posed a low risk to population health, but the dietary lead exposure risk of high-food consumers aged 3-6 and 7-17 posed certain health risks that deserve significant attention.

1116-P: Macronutrient Intake and Risk of Progression to Type 1 Diabetes in TrialNet

Background: Prior research found that children at risk for type 1 diabetes (T1D) with increased dietary intake of sugar and higher glycemic index foods had quicker progression from islet autoimmunity (IA) to T1D diagnosis. However, this has not yet been studied in another population. Objective: To investigate the role of macronutrients in the progression to T1D in TrialNet’s cohort of individuals with IA, including older children and adults. Methods: Baseline dietary data derived from a food frequency questionnaire (FFQ) estimating intake over the past year, and longitudinal clinical data until development of T1D were obtained for individuals with IA from TrialNet’s Pathway to Prevention Study. Proportional hazard regression analysis stratified by age group (1-4 yrs, 5-12 yrs, 13-22 yrs, and 23+ yrs old) was used to evaluate the time from IA detection to T1D diagnosis, with regard to average daily dietary intake of carbohydrates, protein, fat, sugar, fiber, glycemic index, and glycemic load, after adjusting for confounders. Results: A total of 881 individuals age 1-51 years with at least one autoantibody were included, among whom 73/138 toddlers, 160/388 children, 48/166 adolescent/young adults (AYA), and 27/189 adults developed T1D. Given multiple comparisons, there was no significant association between rate of progression from IA to T1D and daily dietary intake of any macronutrients analyzed, after adjusting for baseline age, BMI, and number of autoantibodies, high risk HLA, and time from IA detection to FFQ completion. Borderline association with higher protein intake among AYA (HR 1.04, 95% CI 1.00-1.08) persisted after sensitivity analysis restricted to those with 2+ autoantibodies (HR 1.05, 95% CI 1.01-1.09). Conclusions: Baseline dietary macronutrient intake does not appear to influence rate of T1D diagnosis in children, adolescents, or adults with IA. Further study of dietary factors’ complex role in T1D progression is warranted, including effects of a change in diet over time. Disclosure E. L. Lundgrin: None. A. Viswanathan: None. S. B. Johnson: None. J. M. Norris: None. H. M. Ismail: None. J. R. Wood: Research Support; Self; AstraZeneca, Boehringer Ingelheim Pharmaceuticals, Inc., Insulet Corporation, Speaker’s Bureau; Self; Xeris Pharmaceuticals, Inc. A. J. Thomas: None. D. J. Becker: None. Funding Rainbow Babies &amp; Children’s Foundation; National Institute of Diabetes and Digestive and Kidney Diseases; National Institute of Allergy and Infectious Diseases; Eunice Kennedy Shriver National Institute of Child Health and Human Development; JDRF

Dietary associations with diabetic retinopathy-A cohort study.

Purpose:To assess the role of dietary factors in the development of diabetic retinopathy (DR) in diabetics.Methods:This prospective study was carried out on patients attending the outpatient department of ophthalmology for a period of 1 year. An interview-based 24-hour diet recall was used to document average daily dietary nutrient intakes. Each patient was subjected to a comprehensive ocular examination to look for DR.Results:A total of 261 patients attending the outpatient department of ophthalmology were the participants for this study. The mean (±SD) age of the participants was 57.73 ± 11.29 years, and 67% were men. One hundred and six participants had DR. Univariate analysis revealed sex, duration, fish (times/week), egg (yes/no), rice lunch (yes/no), rice dinner, rice (boiled/white), and total calorie intake to be associated with DR (P < 0.05). Logistic regression multivariable analysis revealed males (OR: 3.20, 95% CI: 1.65–6.19), longer duration of diabetes (OR:1.05,95% CI:1.01-1.11), antioxidant intake (OR: 3.42, 95% CI: 1.65–7.05), and consumption of rice (OR: 3.19, 95% CI: 1.17–8.69) to have significant association with DR (P < 0.05), with the odds of developing DR increasing three times in these patients. The odds of developing DR were lesser with more frequent (>2 times/week) fish consumption (OR: 0.42, 95% CI: 0.18–0.94) and in patients on pharmacological treatment for diabetes mellitus (OR: 0.16, 95% CI: 0.04–0.58). Binary logistic regression revealed chapathi consumption (OR: 9.37, 95% CI: 1.64–53.68) to be associated with severe forms and fish consumption (OR: 0.06, 95% CI: 0.01–1.06) (P < 0.05) to be associated with less severe forms of DR.Conclusion:Males, longer duration of diabetes, antioxidant intake, fish consumption, and consumption of rice were associated with the occurrence of DR. Participants with diabetes who consumed fish more frequently and those who were on pharmacological treatment for diabetes mellitus had a significantly lower risk of DR and frequent fish consumption could reduce the risk of DR progression.