Abstract Background: Partial splenectomy is preferred after splenic injuries, which will help to preserve healthy splenic tissue, hence the immunity. During splenic surgery particularly in the resection of a segment of the spleen, ligation of branches of the splenic artery is necessary. A precise knowledge of the segmental arteries is prerequisite because the spleen is separated into avascular lobes and segments by splenic arterial branches. The aim of the present work was to study the pattern of arterial segmentation in the human spleen and variations of the splenic arterial pattern. Methodology: The present study was conducted on 31 spleens with intact splenic artery after getting the ethical clearance. Specimens were collected depending on the availability of cadaver or unclaimed autopsy body. Tracing from terminal branches, the segmental branches of the splenic artery were studied by piece-meal dissection. The terminal branches of the splenic artery and its segmental branches were cleaned and observed. The number of the segmental branches of the spleen and the presence of arterial anastomosis between adjacent segmental arteries were noted. Results: The splenic artery terminated as bifurcation in 66.73% and trifurcation in 33.27%. We also found two, three, four, five, six, and seven arterial segments in 2 (6.46%), 14 (45.16%), 7 (22.58%), 3 (9.67%), 3 (9.67%), and 2 (6.46%) specimens, respectively. These segments are separated by an avascular plane in all specimens except one where we found intersegmental arterial anastomosis. Conclusions: Knowledge of the splenic arterial branching pattern of the present study may help the general surgeon as well as surgical gastroenterologist in performing the surgery of the spleen, particularly partial splenectomy after splenic injuries. This may also help anatomists in learning and teaching arterial segmentation of the spleen.