Automatic Segmentation System Research Articles

Using a new artificial intelligence-aided method to assess body composition CT segmentation in colorectal cancer patients.

This study aimed to evaluate the accuracy of our own artificial intelligence (AI)-generated model to assess automated segmentation and quantification of body composition-derived computed tomography (CT) slices from the lumber (L3) region in colorectal cancer (CRC) patients. A total of 541 axial CT slices at the L3 vertebra were retrospectively collected from 319 patients with CRC diagnosed during 2012-2019 at a single Australian tertiary institution, Western Health in Melbourne. A two-dimensional U-Net convolutional network was trained on 338 slices to segment muscle, visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT). Manual reading of these same slices of muscle, VAT and SAT was created to serve as ground truth data. The Dice similarity coefficient was used to assess the U-Net-based segmentation performance on both a validation dataset (68 slices) and a test dataset (203 slices). The measurement of cross-sectional area and Hounsfield unit (HU) density of muscle, VAT and SAT were compared between two methods. The segmentation for muscle, VAT and SAT demonstrated excellent performance for both the validation (Dice similarity coefficients >0.98, respectively) and test (Dice similarity coefficients >0.97, respectively) datasets. There was a strong positive correlation between manual and AI segmentation measurements of body composition for both datasets (Spearman's correlation coefficients: 0.944-0.999, P < 0.001). Compared to the gold standard, this fully automated segmentation system exhibited a high accuracy for assessing segmentation and quantification of abdominal muscle and adipose tissues of CT slices at the L3 in CRC patients.

A Novel Deep Learning Approach for Brain Tumor Segmentation and Classification

Automated brain tumor detection and segmentation are crucial in medical diagnostics as they give details about functional structures and also potential unusual tissue that is required to define surgical strategies. Segmentation of images is one of the most difficult issues in the area of MRI scan analysis research. However, due to low contrast, automatic tumor segmentation, accuracy issues, and ill-defined boundaries remain difficult. For the prevention of brain tumors, an investigation was developed for implementing a novel framework. To overcome the problem faced by traditional existing techniques, this paper proposed an automatic brain tumor segmentation and detection system using the proposed dense convolution neural network without human intervention, where an intrinsic set of features are extracted to perform the classification task. The main aim of this paper is to segment the tumor portion from the affected MRI using a novel clustering approach. Moreover, a lot of scholars have researched the diagnosis of brain tumors, yet the accuracy-based performance metric in diagnosis results is low. A deep learning framework is proposed to evaluate brain disease more accurately using a Black Widow Optimization driven Dense Convolution Neural Network (BW-DCNN). The various performances are assessed, and the results are compared to those of other existing classifiers and approaches.

3D auto-segmentation of biliary structure of living liver donors using magnetic resonance cholangiopancreatography for enhanced preoperative planning.

This study aimed to develop an automated segmentation system for biliary structures using a deep learning model, based on data from magnetic resonance cholangiopancreatography (MRCP). Living liver donors who underwent MRCP using the gradient and spin echo technique followed by three-dimensional modeling were eligible for this study. A three-dimensional residual U-Net model was implemented for the deep learning process. Data were divided into training and test sets at a 9:1 ratio. Performance was assessed using the dice similarity coefficient to compare the model's segmentation with the manually labeled ground truth. The study incorporated 250 cases. There was no difference in the baseline characteristics between the train set (n=225) and test set (n=25). The overall mean Dice Similarity Coefficient was 0.80±0.20 between the ground truth and inference result. The qualitative assessment of the model showed relatively high accuracy especially for the common bile duct (88%), common hepatic duct (92%), hilum (96%), right hepatic duct (100%), and left hepatic duct (96%), while the third-order branch of the right hepatic duct (18.2%) showed low accuracy. The developed automated segmentation model for biliary structures, utilizing MRCP data and deep learning techniques, demonstrated robust performance and holds potential for further advancements in automation.

Artificial intelligence system for automatic maxillary sinus segmentation on cone beam computed tomography images.

The study aims to develop an artificial intelligence (AI) model based on nnU-Net v2 for automatic maxillary sinus (MS) segmentation in cone beam computed tomography (CBCT) volumes and to evaluate the performance of this model. In 101 CBCT scans, MS were annotated using the CranioCatch labelling software (Eskisehir, Turkey) The dataset was divided into 3 parts: 80 CBCT scans for training the model, 11 CBCT scans for model validation, and 10 CBCT scans for testing the model. The model training was conducted using the nnU-Net v2 deep learning model with a learning rate of 0.00001 for 1000 epochs. The performance of the model to automatically segment the MS on CBCT scans was assessed by several parameters, including F1-score, accuracy, sensitivity, precision, area under curve (AUC), Dice coefficient (DC), 95% Hausdorff distance (95% HD), and Intersection over Union (IoU) values. F1-score, accuracy, sensitivity, precision values were found to be 0.96, 0.99, 0.96, 0.96, respectively for the successful segmentation of maxillary sinus in CBCT images. AUC, DC, 95% HD, IoU values were 0.97, 0.96, 1.19, 0.93, respectively. Models based on nnU-Net v2 demonstrate the ability to segment the MS autonomously and accurately in CBCT images.

A Cyber-physical-social systems approach to the semantic segmentation of pulmonary embolism

Cyber-Physical-Social Systems (CPSS) epitomize the modern era’s intelligent connectivity. They integrate physical devices, computer networks, and social networks, forming an innovative paradigm for intelligent systems. Utilizing CPSS to enhance intelligence, automation, and remote services in healthcare represents a primary research focus. Pulmonary embolism, a prevalent condition resulting from the blockage of the pulmonary artery and its branches by emboli, leads to a spectrum of clinical syndromes marked by impaired pulmonary circulation and right heart dysfunction, contributing to sudden and unpredictable fatalities. Nevertheless, the diagnosis of pulmonary embolism remains challenging due to non-specific clinical presentations, constrained diagnostic capabilities, delayed diagnoses, insufficient physician knowledge, and suboptimal diagnostic techniques. Consequently, we introduce the innovative LSCU-Net architecture within the CPSS framework, designed to develop an automated segmentation and intelligent assessment system for pulmonary embolism, facilitating its automated and intelligent detection. The experimental findings demonstrate that the model accurately segments pulmonary embolism, evidenced by a Jaccard index of 0.6958, a Dice coefficient of 0.8193, a Mean Pixel Accuracy (mPA) of 0.8519, and an accuracy of 0.9993. Empirical studies reveal that our proposed model substantially surpasses existing models in performance. Consequently, this model can aid physicians in the diagnosis of pulmonary embolism during clinical practice. The established pulmonary embolism automatic segmentation and assessment system also showcases the application successes of CPSS in intelligent remote healthcare. The system’s development and deployment not only streamline physicians’ diagnostic processes but also elevate public health standards and advance CPSS research within the medical domain.

FULLY AUTOMATIC SYSTEM TO DETECT AND SEGMENT THE PROXIMAL FEMUR IN PELVIC RADIOLOGICAL IMAGES FOR LEGG-CALVE-PERTHES DISEASE

The most important outcome predictor of Legg-Calvé-Perthes disease (LCPD) is the shape of the healed femoral head. However, the deformity of the femoral head is currently evaluated by non-reproducible, categorical, and qualitative classifications. In this regard, recent advances in computer vision might provide the opportunity to automatically detect and delineate the outlines of bone in radiographic images for calculating a continuous measure of femoral head deformity. This study aimed to construct a pipeline for accurately detecting and delineating the proximal femur in radiographs of LCPD patients employing existing algorithms. To detect the proximal femur, the pretrained stateof-the-art object detection model, YOLOv5, was trained on 1580 manually annotated radiographs, validated on 338 radiographs, and tested on 338 radiographs. Additionally, 200 radiographs of shoulders and chests were added to the dataset to make the model more robust to false positives and increase generalizability. The convolutional neural network architecture, U-Net, was then employed to segment the detected proximal femur. The network was trained on 80 manually annotated radiographs using real-time data augmentation to increase the number of training images and enhance the generalizability of the segmentation model. The network was validated on 60 radiographs and tested on 60 radiographs. The object detection model achieved a mean Average Precision (mAP) of 0.998 using an Intersection over Union (IoU) threshold of 0.5, and a mAP of 0.712 over IoU thresholds of 0.5 to 0.95 on the test set. The segmentation model achieved an accuracy score of 0.912, a Dice Coefficient of 0.937, and a binary IoU score of 0.854 on the test set. The proposed fully automatic proximal femur detection and segmentation system provides a promising method for accurately detecting and delineating the proximal femoral bone contour in radiographic images, which is necessary for further image analysis.

Improved Word Segmentation System for Chinese Criminal Judgment Documents

ABSTRACT In this paper, a system for automatic word segmentation of Chinese criminal judgment documents is proposed. The system uses a hybrid model composed of fine-tuned BERT (Bidirectional Encoder Representations from Transformers), BiLSTM (Bidirectional Long Short Term Memory) and CRF (Conditional Random Field) for named entity recognition, and introduces a custom dictionary that includes common professional terms in Chinese criminal trial documents, as well as a rule system based on judicial system and litigation procedure related regulations, to further improve the accuracy of word segmentation. BERT uses a deep bidirectional Transformer encoder to pre-train general language representations from large-scale unlabeled text corpora. BiLSTM uses two LSTM networks, one for the forward direction and one for the backward direction, to capture the context from both sides of the input sequence. CRF uses a set of features and weights to define a log-linear distribution over the output sequence. Experimental results show that the proposed system has significantly improved word segmentation accuracy compared to the current commonly used Chinese word segmentation models. In the results of the segmentation of the test data, the F1 scores for jieba, THULAC and the segmentation system proposed in this paper are 85.59%, 87.94% and 94.82%, respectively.

Fully automatic system to detect and segment the proximal femur in pelvic radiographic images for Legg-Calvé-Perthes disease.

This study aimed to develop a method using computer vision techniques to accurately detect and delineate the proximal femur in radiographs of Legg-Calvé-Perthes disease (LCPD) patients. Currently, evaluating femoral head deformity, a crucial predictor of LCPD outcomes, relies on unreliable categorical and qualitative classifications. To address this limitation, we employed the pretrained object detection model YOLOv5 to detect the proximal femur on over 2000 radiographs, including images of shoulders and chests, to enhance robustness and generalizability. Subsequently, we utilized the U-Net convolutional neural network architecture for image segmentation of the proximal femur in more than 800 manually annotated images of stage IV LCPD. The results demonstrate outstanding performance, with the object detection model achieving high accuracy (mean average precision of 0.99) and the segmentation model attaining an accuracy score of 91%, dice coefficient of 0.75, and binary IoU score of 0.85 on the held-out test set. The proposed fully automatic proximal femur detection and segmentation system offers a promising approach to accurately detect and delineate the proximal femoral bone contour in radiographic images, which is essential for further image analysis in LCPD patients.Clinical significance: This study highlights the potential of computer vision techniques for enhancing the reliability ofLegg-Calvé-Perthes diseasestaging and outcome prediction.

A few-shot learning-based ischemic stroke segmentation system using weighted MRI fusion

Stroke, particularly ischemic stroke, is a major cause of disability and one of the leading causes of adult mortality worldwide. Early and prompt management of stroke patients can reduce the severity of the disease. Doctors usually determine the severity of a stroke by focusing on the region of interest (ROI) in the MRI or CT scan images. An accurate and effective automatic image segmentation system can assist medical professionals as well as automatic detection and classification systems. Deep learning is the current advanced approach for dealing with machine learning and artificial intelligence. However, conventional deep learning requires a large amount of data for training, and the amount of labeled data in the medical field is limited. In this paper, we propose a few-shot learning strategy and integrate it with a base convolutional neural network model, which utilizes a self-attention mechanism to segment MRI for ischemic stroke. By combining the base model with self-attention, we can focus more on the ROI and disregard less important features. Additionally, the proposed system only selects slices with lesions and ignores unlesioned slices. This helps to improve efficiency and reduce the computational load by eliminating the need to tune unnecessary parameters. To achieve even better results, the system also combines two weighted images, FLAIR and DWI, as an early fusion process. Experiments have shown that this approach leads to higher performance compared to using the same system without fusion. The proposed system is evaluated using a publicly available dataset, ISLES 2015 SSIS, and compared with other state-of-the-art (SOTA) systems. It achieves a dice coefficient score of 0.68, which is significantly better than that of other SOTA systems.

Deep Learning Models for Automatic Upper Airway Segmentation and Minimum Cross-Sectional Area Localisation in Two-Dimensional Images.

To develop and validate convolutional neural network algorithms for automatic upper airway segmentation and minimum cross-sectional area (CSAmin) localisation in two-dimensional (2D) radiographic airway images. Two hundred and one 2D airway images acquired using cone-beam computed tomography (CBCT) scanning were randomly assigned to a test group (n = 161) to train artificial intelligence (AI) models and a validation group (n = 40) to evaluate the accuracy of AI processing. Four AI models, UNet18, UNet36, DeepLab50 and DeepLab101, were trained to automatically segment the upper airway 2D images in the test group. Precision, recall, Intersection over Union, the dice similarity coefficient and size difference were used to evaluate the performance of the AI-driven segmentation models. The CSAmin height in each image was manually determined using three-dimensional CBCT data. The nonlinear mathematical morphology technique was used to calculate the CSAmin level. Height errors were assessed to evaluate the CSAmin localisation accuracy in the validation group. The time consumed for airway segmentation and CSAmin localisation was compared between manual and AI processing methods. The precision of all four segmentation models exceeded 90.0%. No significant differences were found in the accuracy of any AI models. The consistency of CSAmin localisation in specific segments between manual and AI processing was 0.944. AI processing was much more efficient than manual processing in terms of airway segmentation and CSAmin localisation. We successfully developed and validated a fully automatic AI-driven system for upper airway segmentation and CSAmin localisation using 2D radiographic airway images.

Radiomics-Based Quality Control System for Automatic Cardiac Segmentation: A Feasibility Study.

In the past decade, there has been a rapid increase in the development of automatic cardiac segmentation methods. However, the automatic quality control (QC) of these segmentation methods has received less attention. This study aims to address this gap by developing an automatic pipeline that incorporates DL-based cardiac segmentation and radiomics-based quality control. In the DL-based localization and segmentation part, the entire heart was first located and cropped. Then, the cropped images were further utilized for the segmentation of the right ventricle cavity (RVC), myocardium (MYO), and left ventricle cavity (LVC). As for the radiomics-based QC part, a training radiomics dataset was created with segmentation tasks of various quality. This dataset was used for feature extraction, selection, and QC model development. The model performance was then evaluated using both internal and external testing datasets. In the internal testing dataset, the segmentation model demonstrated a great performance with a dice similarity coefficient (DSC) of 0.954 for whole heart segmentations. Images were then appropriately cropped to 160 × 160 pixels. The models also performed well for cardiac substructure segmentations. The DSC values were 0.863, 0.872, and 0.940 for RVC, MYO, and LVC for 2D masks and 0.928, 0.886, and 0.962 for RVC, MYO, and LVC for 3D masks with an attention-UNet. After feature selection with the radiomics dataset, we developed a series of models to predict the automatic segmentation quality and its DSC value for the RVC, MYO, and LVC structures. The mean absolute values for our best prediction models were 0.060, 0.032, and 0.021 for 2D segmentations and 0.027, 0.017, and 0.011 for 3D segmentations, respectively. Additionally, the radiomics-based classification models demonstrated a high negative detection rate of >0.85 in all 2D groups. In the external dataset, models showed similar results. We developed a pipeline including cardiac substructure segmentation and QC at both the slice (2D) and subject (3D) levels. Our results demonstrate that the radiomics method possesses great potential for the automatic QC of cardiac segmentation.

Deep-learning based system for effective and automatic blood vessel segmentation from Retinal fundus images

Deep-learning based system for effective and automatic blood vessel segmentation from Retinal fundus images

Implementation of Image Quality Improvement Methods and Lung Segmentation on Chest X-Ray Images Using U-Net Architectural Modifications

COVID-19 is an infectious disease that causes acute respiratory distress syndrome due to the SARS-CoV-2 virus. Rapid and accurate screening and early diagnosis of patients play an essential role in controlling outbreaks and reducing the spread of this disease. This disease can be diagnosed by manually reading CXR images, but it is time-consuming and prone to errors. For this reason, this research proposes an automatic medical image segmentation system using a combination of U-Net architecture with Batch Normalization to obtain more accurate and fast results. The method used in this study consists of pre-processing using the CLAHE method and morphology opening, CXR image segmentation using a combination of U-Net-4 Convolution Block architecture with Batch Normalization, then evaluated using performance measures such as accuracy, sensitivity, specificity, F1-score, and IoU. The results showed that the U-Net architecture modified with Batch Normalization had successfully segmented CXR images, as seen from all performance measurement values above 94%.

Clinically Relevant Myocardium Segmentation in Cardiac Magnetic Resonance Images.

Deep learning approaches have shown great success in myocardium region segmentation in Cardiac MR (CMR) images. However, most of these often ignore irregularities such as protrusions, breaks in contour, etc. As a result, the common practice by clinicians is to manually correct the obtained outputs for the evaluation of myocardium condition. This paper aims to make the deep learning systems capable of handling the aforementioned irregularities and satisfy desired clinical constraints, necessary for various downstream clinical analysis. We propose a refinement model which imposes structural constraints on the outputs of the existing deep learning-based myocardium segmentation methods. The complete system is a pipeline of deep neural networks where an initial network performs myocardium segmentation as accurate as possible and the refinement network removes defects from the initial output to make it suitable for clinical decision support systems. We experiment with datasets collected from four different sources and observe consistent final segmentation outputs with improvement up to 8% in Dice Coefficient and up to 18 pixels in Hausdorff Distance due to the proposed refinement model. The proposed refinement strategy leads to qualitative and quantitative improvements in the performances of all the considered segmentation networks. Our work is an important step towards the development of a fully automatic myocardium segmentation system. It can also be generalized for other tasks where the object of interest has regular structure and the defects can be modelled statistically.

Fully-convolutional neural networks ensemble for comet segmentation in single cell gel electrophoresis assay images

ABSTRACT Single-cell gel electrophoresis or comet assay is a simple and reliable method that reveals DNA damage and repair in individual cells. This method is widely used in the medical and biological fields for genotoxicity testing and human biomonitoring studies. Over the past few years, several computational systems have been proposed to perform comet assay image analysis based on traditional image processing methods rather than up-to-date efficient deep learning techniques. Therefore, this work proposes an automatic segmentation system based on a fully-convolutional neural networks ensemble to process comet assay individual cells. Our database was created from peripheral blood samples and consists of comet cells images related to different levels of DNA damage, randomly divided into training, validation and test sets. The experimental results showed that the proposed segmentation model achieves a competitive performance of 87.5% F1-score, 91.5% precision and 84.4% recall. Compared to free-use comet assay analysis systems that apply common thresholding techniques, our model showed more efficient, robust and reliable results. Our model and database are freely available for scientific and academic purposes.

Hepatic vessels segmentation using deep learning and preprocessing enhancement.

Liver hepatic vessels segmentation is a crucial step for the diagnosis process in patients with hepatic diseases. Segmentation of liver vessels helps to study the liver internal segmental anatomy that helps in the preoperative planning of surgical treatment. Recently, the convolutional neural networks (CNN) have been proved to be efficient for the task of medical image segmentation. The paper proposes an automatic deep learning-based system for liver hepatic vessels segmentation of Computed Tomography (CT) datasets from different sources. The proposed work focuses on the combination of different steps; it starts by a preprocessing step to improve the vessels appearance within the liver region of interest in the CT scans. Coherence enhancing diffusion filtering (CED) and vesselness filtering methods are used to improve vessels contrast and intensity homogeneity. The proposed U-net based network architecture is implemented with modified residual block to include concatenation skip connection. The effect of enhancement using filtering step was studied. Also, the effect of data mismatch used in training and validation is studied. The proposed method is evaluated using many CT datasets. Dice similarity coefficient (DSC) is used to evaluate the method. The average DSC score achieved a score 79%. The proposed approach succeeded to segment liver vasculature from the liver envelope accurately, which makes it as potential tool for clinical preoperative planning.

Automatic diagnostic system for segmentation of 3D/2D brain MRI images based on a hardware architecture

Magnetic resonance imaging is among the advanced diagnostic testing tools for brain health issues. This method captures a series of detailed head images. These images are then printed and diagnosed by a specialist doctor to demonstrate differences in the brain tissue. Accordingly, additional diagnostic information can be given to determine the extent of the damage and the appropriate treatment methods. In this paper, and in order to facilitate the work of the specialist doctor and help him, we propose an automated hardware architecture for 3D/2D segmentation on MRI images to diagnose differences in brain tissue. For this, we used the metaheuristic technique based on Particle Swarm Optimization (PSO); for which we proposed improvements both for the velocity and position equations and for the fitness function. The goal of the work is to develop a real time automatic system for MRI images segmentation with improved metrics such as accuracy, sensitivity, specificity, dice metrics, execution time and resources utilization. The proposed hardware architecture was synthetized and then co-simulated using Matlab-Vivado System (VSM) for Field Programmable Gate Array (FPGA). Results show that our 3D segmentation method benefited from 2D segmentation with 95.39% accuracy rate and 87.97% DSC similarity (for 5-level segmentation) with 4.57 ms execution time for the case of BraTS 2013 dataset of brain MRI Images.

Fuzzy K-Nearest Neighbor Based Dental Fluorosis Classification Using Multi-Prototype Unsupervised Possibilistic Fuzzy Clustering via Cuckoo Search Algorithm.

Dental fluorosis in children is a prevalent disease in many regions of the world. One of its root causes is excessive exposure to high concentrations of fluoride in contaminated drinking water during tooth formation. Typically, the disease causes undesirable chalky white or even dark brown stains on the tooth enamel. To help dentists screen the severity of fluorosis, this paper proposes an automatic image-based dental fluorosis segmentation and classification system. Six features from red, green, and blue (RGB) and hue, saturation, and intensity (HIS) color spaces are clustered using unsupervised possibilistic fuzzy clustering (UPFC) into five categories: white, yellow, opaque, brown, and background. The fuzzy k-nearest neighbor method is used for feature classification, and the number of clusters is optimized using the cuckoo search algorithm. The resulting multi-prototypes are further utilized to create a binary mask of teeth and used to segment the tooth region into three groups: white-yellow, opaque, and brown pixels. Finally, a fluorosis classification rule is created based on the proportions of opaque and brown pixels to classify fluorosis into four classes: Normal, Stage 1, Stage 2, and Stage 3. The experimental results on 128 blind test images showed that the average pixel accuracy of the segmented binary tooth mask was 92.24% over the four fluorosis classes, and the average pixel accuracy of segmented teeth into white-yellow, opaque, and brown pixels was 79.46%. The proposed method correctly classified four classes of fluorosis in 86 images from a total of 128 blind test images. When compared with a previous work, this result also indicates 10 out of 15 correct classifications on the blind test images, which is equivalent to a 13.33% improvement over the previous work.

Quantitative analysis of muscle volumes in COVID-19 pneumonia with an automated segmentation system

Aim: The aim of this study was to quantitatively analyze volume of the erector spinae muscle in COVID-19 pneumonia using an artificial intelligence-based automated segmentation program, and to investigate the relationship between pulmonary infiltration ratio and volume of the erector spinae muscle.Methods: In this retrospective study, thoracic CT images of patients who tested positive for SARS-CoV-2 on RT-PCR and had COVID-19 pneumonia were analyzed. Based on the percentage of pulmonary involvement, the study cohort was divided into two groups (Group I: less than 25% involvement and Group II: more than %25 involvement). Volume of the erector spinae muscle and severity of lung involvement were quantitatively analyzed using an artificial intelligence-based automated segmentation program. The data of group I and group II were compared.Results: The study population consisted of 74 subjects; 35 in Group I and 39 in Group II. Significant negative correlations were observed between the total pulmonary infiltration ratio and the volume of the erector spinae muscle. Furthermore, the analysis demonstrated that lung density, total lung infiltration volume, serum C-reactive protein (CRP) level, serum ESR level, and total erector spinae muscle volume can serve as valuable indicators for assessing the severity of lung involvement in patients with COVID-19 pneumonia.Conclusion: Measurement of erector spinae muscle volume may be useful for assessment of pulmonary infiltration in patients with COVID-19 pneumonia

Employing U-NET and Rotational Based Convolutional Neural Network to Build an Automatic Lung Cancer Segmentation and Classification System

The most common cancer-related cause of death globally is lung cancer. The key to effective lung cancer treatment and higher survival rates is early diagnosis. Converting a radiologist's diagnosing procedure to computer assisted results in more accurate results and an earlier diagnosis. The difficulty is that building an effective model for segmentation and classification. In this paper, we suggest a system for detecting lung cancer that makes use of a number of methods for precise and effective diagnosis. To enhance picture quality, our method pre-processes CT scan images using a Gaussian filter and contrast stretching. For the purpose of determining the borders of lung nodules with high precision, the U-Net architecture with the Adam optimizer is used. Then, a Gaussian mixture model (GMM) with EM optimisation and pixel padding is used to extract features. The rotational-based Convolutional Neural Network (RBCNN) classifier successfully categorises the nodules as benign and malignant using these form variables as inputs