Autogenous Tooth Bone Graft Material Research Articles

Guided Bone Regeneration in a Periodontally Compromised Individual with Autogenous Tooth Bone Graft: A Radiomics Analysis.

Autogenous tooth bone graft material (AutoBT) has been advocated as a bone substitute when conducting alveolar ridge preservation. This study is aimed at using a radiomics approach in order to evaluate and testify whether AutoBT can stimulate bone growth during socket preservation in severe periodontal cases. For this study, 25 cases with severe periodontal diseases were selected. The patients' AutoBTs were inserted into the extraction sockets and covered with Bio-Gide® collagen membranes. 3D CBCT scans and 2D X-rays were taken of the patients before surgery and after 6 months post-surgery. For the retrospective radiomics analysis, the maxillary and mandibular images were compared in different groups. Maxillary bone height was analyzed at the buccal, middle, and palatal crest sites, while the mandibular bone height was compared at the buccal, center, and lingual crest sites. In the maxilla, the alveolar height was increased by -2.15 ± 2.90 mm at the buccal crest; -2.45 ± 2.36 mm at the center of the socket, and -1.62 ± 3.19 mm at the palatal crest, while the height of the buccal crest was increased by 0.19 ± 3.52 mm, and the height at the center of the socket was increased by -0.70 ± 2.71 mm in the mandible. The three-dimensional radiomics analysis demonstrated significant bone growth in the local alveolar height and high density. Based on clinical radiomics analysis, AutoBT could be used as an alternative bone material in socket preservation after tooth extraction in patients with severe periodontitis.

Research on the clinical application of autogenous tooth bone graft material

Autogenous tooth bone graft material is made from patients' own useless teeth. Many animal experiments and clinical studies have confirmed that it has good osteoinductivity, osteoconductivity, biocompatibility and other characteristics. This article describes the development and application of autogenous tooth bone graft material.

Conservative Technique Using Oral Dressing Material for Wound Dehiscence after Ridge Augmentation: A Technical Report with a Case Series

Wound dehiscence is the most frequent complication after ridge augmentation and causes postoperative infection, inadequate bone healing, or graft failure. In the oral cavity, conservative treatment for dehiscence is difficult to maintain until secondary healing occurs because of its normal flora, dynamic masticatory muscle movement, and humid environment. This paper reports an effective conservative method using an oral wound dressing material with an omnivec splint and presents three wound dehiscence cases: (1) autogenous tooth bone graft material with a collagen membrane, with dehiscence occurring at postoperative 5 days. (2) Autogenous bone graft covering titanium mesh, with dehiscence occurring at postoperative three weeks. The mesh was removed after 10 weeks with histologic analysis. (3) Autogenous bone and autogenous tooth bone graft covering a titanium mesh, with dehiscence occurring at postoperative 1 week. The exposed titanium mesh was maintained for 6 months after the graft. All cases achieved secondary healing and acceptable outcomes for a dental implant by conservative treatment without infection after the dehiscence after ridge augmentation.

The effect of autogenous tooth bone graft material without organic matter and type I collagen treatment on bone regeneration

ObjectivesThe aim of this study is to examine the effect of particulate autogenous tooth graft removed with organic matter and type I collagen addition on bone regeneration and to validate the possibility of useful allograft material for jaw defects.Material and methodsAutogenous tooth bone maker (Korean Dental Solution® KOREA) made particulate autogenous tooth not including organic matter. We used to the developed tooth grafts for experiment. Cell adhesion test with hemacytometer and energy dispersive X-ray spectroscopy (Supra40 VP®, Carl Zeiss, Germany) analysis about the particulate autogenous tooth and type I collagen were performed. Rabbits were divided into three groups: bone graft with organic matter (OM) removing particulate autogenous tooth group, bone graft with OM removing particulate autogenous tooth and type I collagen group, and a control group. Bone grafting was performed in rabbit’s calvaria. The rabbits were sacrificed at different interval at 1, 2, 4, and 6 weeks after bone grafting for the histopathologic observation and observed the effect of bone regeneration by SEM, H-E & Masson stains, osteocalcin IHC staining.ResultIn vitro cytopathological study showed affinity for cells, cell attachment pattern, and cell proliferation in the order of control group, OM-removed and collagen-treated group, OM-removed particulate autogenous tooth group. The results of the degree of mineralization were opposite to those of the previous cell experimental results, and the OM-removed group, OM-removed group and collagen-treated group were relatively higher than the control group. Histopathologic analysis showed that vascularization and neonatal bone formation were higher in particulate autogenous tooth group with removing OM and with addition of collagen than control group and group of OM removed only. Immunohistochemical analysis showed that osteocalcin (OSC) expression was not observed in the control group, but at 4 weeks groups, OSC expression was observed the OM removed and OM-removed-collagen-treated particulate autogenous tooth, and the degree of expression was somewhat stronger in group of the OM removed and collagen additionally treated particulate autogenous tooth.ConclusionParticles that do not contain organic matter, the saint tooth, was responsible for sufficient bone graft material through the role of space maintenance and bone conduction, and further improved bone formation ability through additional collagen treatment. Therefore, research on various extracellular substrates and autologous bone grafting materials is necessary, and through this, it is possible to lay the foundation for a new type of autologous bone grafting material with excellent academic and technical utility.

Autogenous teeth used for bone grafting: A systematic review.

BackgroundRecently, bone graft materials using permanent teeth have come to light, and clinical and histological outcomes of this material have been confirmed by some studies. The aim of this systematic review was to evaluate the reliability of the autogenous tooth bone graft material applied to alveolar ridge augmentation procedures.Material and MethodsA systematic review of literature was conducted analyzing articles published between 2007 and 2017. The following four outcome variables were defined: a) implant stability b) post-operative complication c) evaluation of implant survival and failure rates, and d) histological analysis. A total of 108 articles were identified; 6 were selected for review. Based on the PICO (problem, intervention, comparison, outcome) model, the chief question of this study was: Can patients with alveolar ridge deficiency be successfully treated with the autogenous teeth used as bone graft?ResultsThe mean primary stability of the placed implants was 67.3 ISQ and the mean secondary stability was 75.5 ISQ. The dehiscence of the wound was the most frequent complication with a rate of 29.1%. Of the 182 analyzed implants, the survival rate was 97.7% and the failure rate was 2.3%. In the histological analysis, most of studies reported bone formation.ConclusionsThere is insufficient evidence regarding the effects of autogenous teeth used for bone grafting to support any definitive conclusions, although it has been shown clinically safe and good bone forming capacity, and good results are shown about implant stability. Key words:Bone regeneration, bone graft material, autogenous teeth graft, tooth.

Evaluation of “Autogenous Bioengineered Injectable PRF – Tooth graft” combination (ABIT) in reconstruction of maxillary alveolar ridge defects: CBCT volumetric analysis

Abstract Background: Extracted human teeth were suggested as a source for autogenous bone grafts, especially that they have similar chemical composition to bone. In order to accelerate healing of bone graft over the bony defect, numerous techniques utilizing platelet and fibrinogen concentrations have been introduced in the literature. Objectives: To evaluate clinically and radiographically the use of autogenous tooth bone graft material in combination with injectable platelet-rich fibrin for reconstruction of resorbed maxillary alveolar ridge. Patients and methods: This study was conducted in ten patients indicated for extraction of maxillary anterior or premolar teeth and having maxillary ridge deficiency. Implants were placed after a period of 3 months of bone grafting. The patients were selected from those attending the Oral and Maxillofacial Surgery Department, Faculty of Dentistry, Alexandria University. Results: Clinically, all the patients showed uneventful healing. Radiographically, the results show 30% increase in mean bone density in the period of six months when compared to pre-operative readings. The grafted site showed an increase in the mean volume by 23.47% three months post-operative when compared to the base line measurements preoperatively. Radiographic evaluation of the alveolar ridge after 3 months of grafting showed a significant mean increase in the ridge width and height at the grafted site. Conclusion: Autogenous fresh demineralized tooth graft that is prepared at the chairside after extractions could be considered as the gold standard for socket preservation, bone augmentation in sinuses or filling bone defects, in patients having non restorable teeth indicated for extraction. The adjuvant use of injectable PRF with its high growth factor content may contribute to more favorable and predictable bone formation at the grafted site.

Applications of Moldable Autogenous Tooth Bone Graft (M-AutoBT) Mixed with Hydroxypropylmethyl Cellulose for Sinus Lifting

The purpose of this study is to evaluate an ability of a moldable autogenous tooth bone graft material (M-AutoBT), which is made of demineralized dentin matrix (DDM) and hydroxypropylmethyl cellulose (HPMC) to repair sinus-related defects in the implant dentistry. Sinus-related alveolar defects were classified into three types depending on the remnant of socket wall as follows; Class I: Only alveolar bone repair is required even if sinus cavity is involved (remained socket wall > 5mm). Class II: Both alveolar bone and sinus repair are required (3mm < remained socket wall < 5mm). Class III: Only sinus repair is required without any alveolar bone (remained socket wall < 3mm). Sinus cavity should always be involved during the implant placement regardless of defect types. The histological examination of three case reports, which represented class I, II and III defect respectively, showed osteoconductive and osteoinductive functions of M-AutoBT that is similar to the effect of the conventional AutoBT. Therefore, HPMC might be the suitable base material for AutoBT.

The Efficacy of the Graft Materials after Sinus Elevation: Retrospective Comparative Study Using Panoramic Radiography.

Purpose:This study compares and evaluates the efficacy of graft materials after maxillary sinus bone grafts with autogenous tooth bone graft material (AutoBT), demineralized freeze-dried bone allograft (DFDBA) and deproteinized bovine bone mineral (DBBM).Methods:The study involved 30 sinuses in 26 patients who visited the Division of Oral and Maxillofacial Surgery, Department of Dentistry in Ajou University Hospital and received either AutoBT, DFDBA or DBBM with sinus elevation using the lateral window technique. Sinus graft height was measured before, immediately after, and six months after bone graft with panoramic radiography and the height changes of the sinus floor was compared according to the graft materials.Results:After six months, the decrease ratio of graft heights were 13.57% for AutoBT group, 14.30% for DFDBA group, and 11.92% for DBBM group. There was no statistically significant difference.Conclusion:The new maxillary sinus floor formed by the upper border of bone graft material, can repneumatize after the maxillary sinus elevation. Thus, long-term stability of sinus graft height represents an important factor for implant success. We found that the three graft materials for sinus elevation do not differ significantly and all three graft materials showed excellent resistance to maxillary sinus repneumatization. However, due to the special circumstances of the maxillary sinus and small sample, the actual difference between the three graft materials may not have been detectable. Therefore further study needs to be conducted for more reliable study results.

Alveolar ridge preservation of an extraction socket using autogenous tooth bone graft material for implant site development: prospective case series.

This case series evaluated the clinical efficacy of autogenous tooth bone graft material (AutoBT) in alveolar ridge preservation of an extraction socket. Thirteen patients who received extraction socket graft using AutoBT followed by delayed implant placements from Nov. 2008 to Aug. 2010 were evaluated. A total of fifteen implants were placed. The primary and secondary stability of the placed implants were an average of 58 ISQ and 77.9 ISQ, respectively. The average amount of crestal bone loss around the implant was 0.05 mm during an average of 22.5 months (from 12 to 34 months) of functional loading. Newly formed tissues were evident from the 3-month specimen. Within the limitations of this case, autogenous tooth bone graft material can be a favorable bone substitute for extraction socket graft due to its good bone remodeling and osteoconductivity.

A prospective study on the effectiveness of newly developed autogenous tooth bone graft material for sinus bone graft procedure.

PURPOSEThe purpose of this prospective study was to evaluate the effectiveness of newly developed autogenous tooth bone graft material (AutoBT)application for sinus bone graft procedure.MATERIALS AND METHODSThe patients with less than 5.0 mm of residual bone height in maxillary posterior area were enrolled. For the sinus bone graft procedure, Bio-Oss was grafted in control group and AutoBT powder was grafted in experimental group. Clinical and radiographic examination were done for the comparison of grafted materials in sinus cavity between groups. At 4 months after sinus bone graft procedure, biopsy specimens were analyzed by microcomputed tomography and histomorphometric examination for the evaluation of healing state of bone graft site.RESULTSIn CT evaluation, there was no difference in bone density, bone height and sinus membrane thickness between groups. In microCT analysis, there was no difference in total bone volume, new bone volume, bone mineral density of new bone between groups. There was significant difference trabecular thickness (0.07 µm in Bio-Oss group Vs. 0.08 µm in AutoBT group) (P=.006). In histomorphometric analysis, there was no difference in new bone formation, residual graft material, bone marrow space between groups. There was significant difference osteoid thickness (8.35 µm in Bio-Oss group Vs. 13.12 µm in AutoBT group) (P=.025).CONCLUSIONAutoBT could be considered a viable alternative to the autogenous bone or other bone graft materials in sinus bone graft procedure.

Analysis of Organic Components and Osteoinductivity in Autogenous Tooth Bone Graft Material

Purpose: Extensive research is actively ongoing for development of an ideal bone substitute that meets the gold standard. Tooth was selected as a donor site for evaluation of potentials in bone substitutes based on its similar chemical compositions to alveolar bone. Previous studies have evaluated inorganic components of autogenous tooth bone graft material (AutoBT) and osteoconductivity. In continuation from the previous studies, the current study was conducted for analysis of organic components and evaluation of osteoinductivity of AutoBT. Methods: Forty-six extracted teeth were collected from actual patients (Korea Tooth Bank, RD 0.02% (0.029 mg/g) and 1.79% (17.93 mg/g) of proteins were measured by weight in crown and block-form of AutoBT, respectively. However, recombinant human bone morphogenetic protein-2 was not observed in AutoBT. Conclusion: Within the limitation of the current study, AutoBT induced new bone formation by NCP embedded in dentin.

Evaluation of the Healing Process of Autogenous Tooth Bone Graft Material Nine Months after Sinus Bone Graft: Micromorphometric and Histological Evaluation

Micromorphometric and histological examinations were conducted with a collected tissue specimen nine months after sinus bone graft using autogenous tooth bone graft material (AutoBT). As a result of micro-computed tomographic analysis, the total bone volume (graft material+new bone) was 76.45%, and the proportion of new bone was 45.4%. The bone mineral density and the average Hounsfield Unit of new bone were 0.26 and 1,164.69, respectively. The histological examination showed that AutoBT particles were united well with new bone. AutoBT was considered to have excellent bone healing ability after sinus graft and bone density that can resist repneumatization.

자가치아 뼈 이식재의 유용성에 관한 임상적 연구

Abstract Clinical Study on the Efficacy of the Autogenous Tooth Bone Graft Material (AutoBT) Min Woo Han, Jeong Keun Lee Division of Oral and Maxillofacial Surgery, Department of Denti stry, Ajou University School of MedicinePurpose: This study is to evaluate the efficacy of the autogenous tooth bone graft material, clinically and radiologically, as related to implant installation.Methods: In oral and maxillofacial surgery department of Ajou University Hospital, guided bone regeneration (GBR), implant placement combined with GBR, sinus graft, implant placement com bined with sinus graft, and defect filling were performed in 46 patients, using autogenous tooth bone. Among these, 66 im plants were inserted with autogenous tooth bone. Implant stability quotient (ISQ) was measured by Osstell Mentor (Integration Diag nostics, Goteborg, Sweden) on 39 implants on the operation date and 4 months later, and on 21 implants 9months o n the average at the final setting of restoration. Twenty-eight implants with GBR and sinus graft (GBR group: n=14, sinus graft group: n=14) were evaluated radiologically to measure the resorption of grafted autogenous tooth bone after loading.Results: The average initial stabilization of the installed implants was 67 ISQ, and the average secondary stab ilization at 4 months later was 76. The average bone loss of GBR group as me asured 8.0 months after application of prosthesis loading was 0.29 mm and the average bone loss of the sinus graft group as measured 7.6 months after application of prosthesis loading was 0.66 mm, respectively. In the histological assessme nt, formation of the new bone and continuous trabecular bone pattern was identified around autogenous tooth bone.Conclusion: Based on these results, we concluded that autogenous tooth bon e is an excellent bone graft material that can substitute the autogenous bone.Key words:Autogenous tooth bone graft material, Efficacy, Dental implant

Alveolar Cleft Reconstruction Using Chin Bone and Autogenous Tooth Bone Graft Material: Reports of 5 Cases

Purpose: To report the successful results of using chin bone graft and autogenous tooth bone graft material (AutoBT) in alveolar cleft patients. Materials and Methods: Five patients with alveolar cleft defects underwent alveolar bone grafting. Three patients were treated using chin bone graft, and the other two patients underwent AutoBT graft. After implant site development using chin bone graft in the fi rst three cases, endosseous implant restorations were placed. In case #4 and 5, AutoBT graft material was placed to guide the normal eruption of partially impacted maxillary right canine and to the upper docking site after distraction osteogenesis. Result: Successful implant restorations with closure of the oronasal fistula were achieved in alveolar cleft defect reconstruction using either chin bone graft (Case #1, 2, 3) or AutoBT graft material (Case #4, 5). Case #4 showed enlarged follicle of the right maxillary canine, indicating a normal eruption guide pattern. Conclusion: Both chin bone graft and AutoBT graft showed favorable outcomes in reconstructing alveolar cleft defects. Autogenous tooth bone graft opens up the possibility of avoiding harvesting autogenous bone graft with complications and morbidities.

Comparative analysis of guided bone regeneration using autogenous tooth bone graft material with and without resorbable membrane

Background/purposeThe use of membrane in preventing soft tissue ingrowth during guided bone regeneration (GBR) procedure for better clinical results is controversial. The present study compares and analyzes the clinical results of GBR using the autogenous tooth bone graft (AutoBT; Korea Tooth Bank Co., Seoul, Korea) material with and without the resorbable membrane (Bio-Arm, ACE Surgical. Supply Company, Inc., USA). Materials and methodsPatients who received dental implants with simultaneous GBR from the same clinician at the Dental Department of Seoul National University Bundang Hospital from March 2009 to May 2012 were selected in this study. A total of 20 patients with a total number of 30 dental implants were included in this study. The patients who received GBR with resorbable membrane were in Group 1 and those without membrane were in Group 2. AutoBT was grafted in all patients. In each group, pre- and postoperative bone loss, regeneration in percentage (%), and complications were evaluated. ResultsThere was no statistically significant difference in pre- and postoperative reduction of bone defect height, bone level change, and bone regeneration in percentage (%) between the two groups (P > 0.05). ConclusionBoth groups showed clinically acceptable bone regeneration without any eventful complications. Within the limitation of this study, we can carefully conclude that the use of resorbable membrane is not a critical factor in GBR when using AutoBT.

Analysis of the Inorganic Component of Autogenous Tooth Bone Graft Material

Analysis of the Inorganic Component of Autogenous Tooth Bone Graft Material

Clinical evaluation of ridge augmentation using autogenous tooth bone graft material: case series study

ObjectivesInterest in bone graft material has increased with regard to restoration in cases of bone defect around the implant. Autogenous tooth bone graft material was developed and commercialized in 2008. In this study, we evaluated the results of vertical and horizontal ridge augmentation with autogenous tooth bone graft material.Materials and MethodsThis study targeted patients who had vertical or horizontal ridge augmentation using AutoBT from March 2009 to April 2010. We evaluated the age and gender of the subject patients, implant stability, adjunctive surgery, additional bone graft material and barrier membrane, post-operative complication, implant survival rate, and crestal bone loss.ResultsWe performed vertical and horizontal ridge augmentation using powder- or block-type autogenous tooth bone graft material, and implant placement was performed on nine patients (male: 7, female: 2). The average age of patients was 49.88±12.98 years, and the post-operative follow-up period was 35±5.31 months. Post-operative complications included wound dehiscence (one case), hematoma (one case), and implant osseointegration failure (one case; survival rate: 96%); however, there were no complications related to bone graft material, such as infection. Average marginal bone loss after one-year loading was 0.12±0.19 mm. Therefore, excellent clinical results can be said to have been obtained.ConclusionExcellent clinical results can be said to have been obtained with vertical and horizontal ridge augmentation using autogenous tooth bone graft material.

Autogenous teeth used for bone grafting: a comparison with traditional grafting materials

This study evaluated the surface structures and physicochemical characteristics of a novel autogenous tooth bone graft material currently in clinical use. The material's surface structure was compared with a variety of other bone graft materials via scanning electron microscope (SEM). The crystalline structure of the autogenous tooth bone graft material from the crown (AutoBT crown) and root (AutoBT root), xenograft (BioOss), alloplastic material (MBCP), allograft (ICB), and autogenous mandibular cortical bone were compared using x-ray diffraction (XRD) analysis. The solubility of each material was measured with the Ca/P dissolution test. The results of the SEM analysis showed that the pattern associated with AutoBT was similar to that from autogenous cortical bones. In the XRD analysis, AutoBT root and allograft showed a low crystalline structure similar to that of autogenous cortical bones. In the CaP dissolution test, the amount of calcium and phosphorus dissolution in AutoBT was significant from the beginning, while displaying a pattern similar to that of autogenous cortical bones. In conclusion, autogenous tooth bone graft materials can be considered to have physicochemical characteristics similar to those of autogenous bones.

Bone Healing Capacity of Demineralized Dentin Matrix Materials in a Mini-pig Cranium Defect

Purpose: In this study the bone healing ability of autogenous tooth bone graft material as a substitute material was evaluated in a mini-pig cranial defect model through histologic examinations and osteonectin reverse transcription polymerase chain reaction (RT-PCR) quantitative analysis. Materials and Methods: A defect was generated in the cranium of mini-pigs and those without a defect were used as controls. In the experimental group, teeth extracted from the mini-pig were manufactured into autogenous tooth bone graft material and grafted to the defect. The mini-pigs were sacrificed at 4, 8, and 12 weeks to histologically evaluate bone healing ability and observe the osteonectin gene expression pattern with RT-PCR. Result: At 4 weeks, the inside of the bur hole showed fibrosis and there was no sign of bone formation in the control group. On the other hand, bone formation surrounding the tooth powder granule was observed at 4 weeks in the experimental group where the bur hole was filled with tooth powder. Osteonectin gene expression; there was nearly no osteonectin expression in the control group while active osteonectin expression was observed from 4 to 12 weeks in the experimental group. Conclusion: We believe this material will show better results when applied in a clinical setting.

Bone graft material using teeth

Autogenous tooth bone graft material contains organic and inorganic components for osteoinductive and osteoconductive healing. The clinical availability and safety of this material have been confirmed by various experimental and clinical studies. In the future, allogenic and xenogenic tooth bone graft materials, ideal scaffold using teeth for stem cells and bone growth factors, and endodontic and tooth restorative material will be developed.