Thangka, a masterpiece of Tibetan painting, is renowned for its adept use of natural mineral pigments such as gold, turquoise, and cinnabar, which imbue it with profound artistic and historical significance. Presently, chemical analysis methods relying on microscopic perspectives are prevalent in researching the pigment components of cultural artifacts. However, these methods suffer from quantization gaps and carry the risk of damaging the relics. Hence, in this study, we focus on Thangka Five Buddha as our experimental sample and propose a novel approach utilizing Linear Spectral Mixed Analysis (LSMA) based on Hyperspectral Imaging (HSI) technology to perform quantitative analysis of pigment components at a sub-pixel level. The results indicate the following. 1) A database of spectral curves for 25 representative Thangka pigments was established, covering 196 bands from 393 to 800 nm VIR-NIR range. 2) The LSMA model successfully separated the 13 pigment components of the Thangka at the sub-pixel level, achieving a Root Mean Square Error (RMSE) of 0.0186, which indicates high classification accuracy. 3) The quantitative analysis reveals that 33.07 % of the area is painted using a single pigment, while 56.01 % is painted using a combination of two pigments. Verdigris (18.56 %), malachite (17.52 %), and cinnabar (10.91 %) are the pigment types with the highest proportions among them. Out of the 521 pigment combinations, verdigris and turquoise (4.55 %), malachite and calcite (4.02 %), minium and cinnabar (2.87 %), and turquoise and malachite (2.82 %) are more commonly used. 4) The application of quantitative analysis methods demonstrates significant potential in painting techniques, authentication processes, and establishing historical dating, among other areas of study.