Austronesian-speaking Groups Research Articles

Reanalyzing the genetic history of Kra-Dai speakers from Thailand and new insights into their genetic interactions beyond Mainland Southeast Asia

Thailand is a country where over 60 languages from five language families (Austroasiatic, Austronesian, Hmong-Mien, Kra-Dai, and Sino-Tibetan) are spoken. The Kra-Dai language family is the most prevalent, and Thai, the official language of the country, belongs to it. Previous genome-wide studies on Thailand populations revealed a complex population structure and put some hypotheses forward concerning the population history of the country. However, many published populations have not been co-analyzed, and some aspects of population history were not explored adequately. In this study, we employ new methods to re-analyze published genome-wide genetic data on Thailand populations, with a focus on 14 Kra-Dai-speaking groups. Our analyses reveal South Asian ancestry in Kra-Dai-speaking Lao Isan and Khonmueang, and in Austroasiatic-speaking Palaung, in contrast to a previous study in which the data were generated. We support the admixture scenario for the formation of Kra-Dai-speaking groups from Thailand who harbor both Austroasiatic-related ancestry and Kra-Dai-related ancestry from outside of Thailand. We also provide evidence of bidirectional admixture between Southern Thai and Nayu, an Austronesian-speaking group from Southern Thailand. Challenging some previously reported genetic analyses, we reveal a close genetic relationship between Nayu and Austronesian-speaking groups from Island Southeast Asia (ISEA).

The first data of allele frequencies for 23 autosomal STRs in the Ede ethnic group in Vietnam

Settled in Vietnam, the Ede ethnic group exhibits unique heritages that belong to the Austronesian-speaking groups. In this study, we genotyped 23 autosomal short tandem repeat (STR) markers in 397 unrelated samples from the Ede population and recorded the first data of allele frequencies and forensic parameters for the Ede. Data analysis revealed high values of the combined discrimination power (0.999 999 999 999 999 999 999 999 950) and the exclusion power (0.999 999 999 999 998). Within the Ede population, D12S391 was indicated to be an informative autosomal STR marker for individual identification and paternity testing. A phylogenetic tree of the Ede and nine other Austronesian groups, spanning from Africa to Asia and Oceania, asserted a close relationship among these groups, especially among the Ede, Malaysian Malay, and Indonesian populations.

Negritos in Taiwan and the wider prehistory of Southeast Asia: new discovery from the Xiaoma Caves

ABSTRACTTaiwan is known as the homeland of the Austronesian-speaking groups, yet other populations already had lived here since the Pleistocene. Conventional notions have postulated that the Palaeolithic hunter-gatherers were replaced or absorbed into the Neolithic Austronesian farming communities. Yet, some evidence has indicated that sparse numbers of non-Austronesian individuals continued to live in the remote mountains as late as the 1800s. The cranial morphometric study of human skeletal remains unearthed from the Xiaoma Caves in eastern Taiwan, for the first time, validates the prior existence of small stature hunter-gatherers 6000 years ago in the preceramic phase. This female individual shared remarkable cranial affinities and small stature characteristics with the Indigenous Southeast Asians, particularly the Negritos in northern Luzon. This study solves the several-hundred-years-old mysteries of ‘little black people’ legends in Formosan Austronesian tribes and brings insights into the broader prehistory of Southeast Asia.

Afrikai citerák

A general historical survey of African zither types cannot fail to highlight the disproportionalities brought about in the study of Africa by the essentialistic ideology of Afrocentrism. Thus the widely known videoclip of the 1987 hit Yé-ké-yé-ké by the late Mory Kante (d. 22nd May 2020), musician and composer of Guinean Mandinka origin has allowed millions to experience the kora harp lute with which he accompanied his song and popularized this instrument as well as the musical tradition of the West African griots, while the obviously related mvet harp zither is scarcely known today. This despite the fact that both the latter instrument type and its specialists, the mbomo mvet master singers, played a very similar role in the cultures of the Central African chiefdoms, as did the nanga bards playing the enanga trough zither in the East African kingdoms. Another important and interesting historical insight provided by a careful morphological and etymological analysis of African zither types and their terminology that takes comparative account of South and Southeast Asian data and ethnographic parallels concerns the possibility of borrowings. Thus stick and raft zither types may well have reached the eastern half of West Africa and the northeastern part of Central Africa – several centuries prior to the era of European geographical explorations – owing to population movements over the Red Sea. It seems therefore probable that the African stick bridges harp zithers (in fact a sui generis instrument type rather than a subtype of zithers) developed from South Asian stick zither types. On the other hand, tube zithers and box zithers – fretted-enhanced versions of the stick zither – certainly reached Africa because of the migration of Austronesian-speaking groups over the Indian Ocean, since their recent ethnographic analogies have survived in Southeast Asia as well. By contrast types of trough zither, confined to East Africa, must have developed in Africa from box zither types, which are based on similar techniques of making the strings tense. The hypothesis of African zither types having originated from beyond the Indian Ocean is further strengthened by the absence of these instruments in such regions of Sub-Saharan Africa as the Atlantic coast of West Africa as well as in Northeast, Southwest and South Africa. Thus the historical overview of African zither types also helps refute the erroneous idea that prior to the arrival of European explorers and colonizers the continent was isolated from the rest of the world. In fact seafaring peoples such as the Austronesians, Chinese, Indians, Arabs and Persians did continually reach it, bringing with them cultural artifacts, production techniques and agricultural products among other things, which would then spread over large distances along the trade routes over Africa.

Ritual tooth ablation and the Austronesian expansion: Evidence from eastern Indonesia and the Pacific Islands

Ritual tooth ablation, the intentional removal of teeth, is a highly visible form of body modification that can signal group identity and mark certain life events, such as marriage. The widespread occurrence of the practice in Asia appears to have begun in the Neolithic period and in some areas, such as Taiwan, continued until the ethnographic present. We aim to use a biocultural approach to investigate the significance of tooth ablation in Indonesia and Vanuatu during the maritime expansion of Austronesian-speaking groups ca. 3500–2000 years ago. Here we assess the presence and patterns of tooth ablation in four prehistoric skeletal assemblages from eastern Indonesia (Pain Haka, Melolo, Lewoleba and Liang Bua) and one from Vanuatu (Uripiv). Despite the relatively small sample sizes, it was found that individuals from all the sites displayed tooth ablation. The Indonesian populations had ablation patterns that involved the maxillary lateral incisors and canines and the individuals from Uripiv had the central maxillary incisors removed. We suggest that the distribution of tooth ablation in eastern Indonesia provides strong evidence that this practice was an important ritual process associated with the early expansion of Austronesian-speaking populations in the region. The identification of tooth ablation at the site of Uripiv is the earliest example of the practice in the Pacific Islands and was either a Southeast Asian tradition brought by Austronesian settlers, was introduced later from Near Oceania, or was an indigenous development in Vanuatu. A similar pattern of tooth ablation (the removal of central maxillary incisors) has been documented in ethnographic reports of northern Vanuatu tribes. We argue that the practice could possibly be a ritual passed through the generations since the early settlement of Vanuatu.

The paternal and maternal genetic history of Vietnamese populations

Vietnam exhibits great cultural and linguistic diversity, yet the genetic history of Vietnamese populations remains poorly understood. Previous studies focused mostly on the majority Kinh group, and thus the genetic diversity of the many other groups has not yet been investigated. Here we analyze complete mtDNA genome sequences and ~2.3 Mb sequences of the male-specific portion of the Y chromosome from the Kinh and 16 minority populations, encompassing all five language families present in Vietnam. We find highly variable levels of diversity within and between groups that do not correlate with either geography or language family. In particular, the Mang and Sila have undergone recent, independent bottlenecks, while the majority group, Kinh, exhibits low levels of differentiation with other groups. The two Austronesian-speaking groups, Giarai and Ede, show a potential impact of matrilocality on their patterns of variation. Overall, we find that isolation, coupled with limited contact involving some groups, has been the major factor influencing the genetic structure of Vietnamese populations, and that there is substantial genetic diversity that is not represented by the Kinh.

Az afrikai tamtam-ok avagy résdobok története és típusai

A general survey of data leads one to conclude that, even though the slit drum type of instruments, owing to their simple structure, could have developed in any place with abundant tree cover, the available data suggest that the introduction of this instrument type, or the underlying idea, into the equatorial belt can be linked to the migration of speakers of Austronesian languages. After its introduction, however, the instrument type developed in highly localized fnctions and forms. On this basis the widespread slit drum types of Equatorial Africa were passed on in an East-to-West direction; the absence of these instruments in Southern Africa and the date of arrival of Austronesian-speaking groups in Africa suggest the possibility that the appearance of slit drums in Africa can be dated to the period between the 6th and 10th centuries. The communicative function and prestige value of this instrument type led to its wide adoption among the ethnic groups of the Congo basin in particular. Contrastingly, a major hindrance to its wider distribution over West Africa may have been the presence of a rival candidate for its communicative function (the membranophonic „talking drums”), as well as changes in the animistic religious concepts linked to this instrument type, a result of widepread conversion to Islam from the 14th century. In Eastern Africa too conversion to Islam from the 11th century and the associated spead of the use of Swahili may have been a significant factor, given the fact that Swahili, while a Bantu language, is not a tone language, and thus the diminished communicative potential of these instruments, dependent on tone, as well as the shrinking ritual aspects of their use as symbols of chiefly authority must have led to the gradual decline of the use of slit drums in this region.

New insights from Thailand into the maternal genetic history of Mainland Southeast Asia

Tai-Kadai (TK) is one of the major language families in Mainland Southeast Asia (MSEA), with a concentration in the area of Thailand and Laos. Our previous study of 1234 mtDNA genome sequences supported a demic diffusion scenario in the spread of TK languages from southern China to Laos as well as northern and northeastern Thailand. Here we add an additional 560 mtDNA genomes from 22 groups, with a focus on the TK-speaking central Thai people and the Sino-Tibetan speaking Karen. We find extensive diversity, including 62 haplogroups not reported previously from this region. Demic diffusion is still a preferable scenario for central Thais, emphasizing the expansion of TK people through MSEA, although there is also some support for gene flow between central Thai and native Austroasiatic speaking Mon and Khmer. We also tested competing models concerning the genetic relationships of groups from the major MSEA languages, and found support for an ancestral relationship of TK and Austronesian-speaking groups.

Taiwan Y-chromosomal DNA variation and its relationship with Island Southeast Asia.

BackgroundMuch of the data resolution of the haploid non-recombining Y chromosome (NRY) haplogroup O in East Asia are still rudimentary and could be an explanatory factor for current debates on the settlement history of Island Southeast Asia (ISEA). Here, 81 slowly evolving markers (mostly SNPs) and 17 Y-chromosomal short tandem repeats were used to achieve higher level molecular resolution. Our aim is to investigate if the distribution of NRY DNA variation in Taiwan and ISEA is consistent with a single pre-Neolithic expansion scenario from Southeast China to all ISEA, or if it better fits an expansion model from Taiwan (the OOT model), or whether a more complex history of settlement and dispersals throughout ISEA should be envisioned.ResultsWe examined DNA samples from 1658 individuals from Vietnam, Thailand, Fujian, Taiwan (Han, plain tribes and 14 indigenous groups), the Philippines and Indonesia. While haplogroups O1a*-M119, O1a1*-P203, O1a2-M50 and O3a2-P201 follow a decreasing cline from Taiwan towards Western Indonesia, O2a1-M95/M88, O3a*-M324, O3a1c-IMS-JST002611 and O3a2c1a-M133 decline northward from Western Indonesia towards Taiwan. Compared to the Taiwan plain tribe minority groups the Taiwanese Austronesian speaking groups show little genetic paternal contribution from Han. They are also characterized by low Y-chromosome diversity, thus testifying for fast drift in these populations. However, in contrast to data provided from other regions of the genome, Y-chromosome gene diversity in Taiwan mountain tribes significantly increases from North to South.ConclusionThe geographic distribution and the diversity accumulated in the O1a*-M119, O1a1*-P203, O1a2-M50 and O3a2-P201 haplogroups on one hand, and in the O2a1-M95/M88, O3a*-M324, O3a1c-IMS-JST002611 and O3a2c1a-M133 haplogroups on the other, support a pincer model of dispersals and gene flow from the mainland to the islands which likely started during the late upper Paleolithic, 18,000 to 15,000 years ago. The branches of the pincer contributed separately to the paternal gene pool of the Philippines and conjointly to the gene pools of Madagascar and the Solomon Islands. The North to South increase in diversity found for Taiwanese Austronesian speaking groups contrasts with observations based on mitochondrial DNA, thus hinting to a differentiated demographic history of men and women in these populations.

Bridging Near and Remote Oceania: mtDNA and NRY Variation in the Solomon Islands

Although genetic studies have contributed greatly to our understanding of the colonization of Near and Remote Oceania, important gaps still exist. One such gap is the Solomon Islands, which extend between Bougainville and Vanuatu, thereby bridging Near and Remote Oceania, and include both Austronesian-speaking and Papuan-speaking groups. Here, we describe patterns of mitochondrial DNA (mtDNA) and nonrecombining Y chromosome (NRY) variation in over 700 individuals from 18 populations in the Solomons, including 11 Austronesian-speaking groups, 3 Papuan-speaking groups, and 4 Polynesian Outliers (descended via back migration from Polynesia). We find evidence for ancient (pre-Lapita) colonization of the Solomons in old NRY paragroups as well as from M2-M353, which probably arose in the Solomons ∼9,200 years ago and is the most frequent NRY haplogroup there. There are no consistent genetic differences between Austronesian-speaking and Papuan-speaking groups, suggesting extensive genetic contact between them. Santa Cruz, which is located in Remote Oceania, shows unusually low frequencies of mtDNA and NRY haplogroups of recent Asian ancestry. This is in apparent contradiction with expectations based on archaeological and linguistic evidence for an early (∼3,200 years ago), direct colonization of Santa Cruz by Lapita people from the Bismarck Archipelago, via a migration that "leapfrogged" over the rest of the Solomons. Polynesian Outliers show dramatic island-specific founder events involving various NRY haplogroups. We also find that NRY, but not mtDNA, genetic distance is correlated with the geographic distance between Solomons groups and that historically attested spheres of cultural interaction are associated with the recent genetic structure of Solomons groups, as revealed by mtDNA HV1 sequence and Y-STR haplotype diversity. Our results fill an important lacuna in human genetic studies of Oceania and aid in understanding the colonization and genetic history of this region.

The Impact of the Austronesian Expansion: Evidence from mtDNA and Y Chromosome Diversity in the Admiralty Islands of Melanesia

The genetic ancestry of Polynesians can be traced to both Asia and Melanesia, which presumably reflects admixture occurring between incoming Austronesians and resident non-Austronesians in Melanesia before the subsequent occupation of the greater Pacific; however, the genetic impact of the Austronesian expansion to Melanesia remains largely unknown. We therefore studied the diversity of nonrecombining Y chromosomal (NRY) and mitochondrial (mt) DNA in the Admiralty Islands, located north of mainland Papua New Guinea, and updated our previous data from Asia, Melanesia, and Polynesia with new NRY markers. The Admiralties are occupied today solely by Austronesian-speaking groups, but their human settlement history goes back 20,000 years prior to the arrival of Austronesians about 3,400 years ago. On the Admiralties, we found substantial mtDNA and NRY variation of both Austronesian and non-Austronesian origins, with higher frequencies of Asian mtDNA and Melanesian NRY haplogroups, similar to previous findings in Polynesia and perhaps as a consequence of Austronesian matrilocality. Thus, the Austronesian language replacement on the Admiralties (and elsewhere in Island Melanesia and coastal New Guinea) was accompanied by an incomplete genetic replacement that is more associated with mtDNA than with NRY diversity. These results provide further support for the "Slow Boat" model of Polynesian origins, according to which Polynesian ancestors originated from East Asia but genetically mixed with Melanesians before colonizing the Pacific. We also observed that non-Austronesian groups of coastal New Guinea and Island Melanesia had significantly higher frequencies of Asian mtDNA haplogroups than of Asian NRY haplogroups, suggesting sex-biased admixture perhaps as a consequence of non-Austronesian patrilocality. We additionally found that the predominant NRY haplogroup of Asian origin in the Admiralties (O-M110) likely originated in Taiwan, thus providing the first direct Y chromosome evidence for a Taiwanese origin of the Austronesian expansion. Furthermore, we identified a NRY haplogroup (K-P79, also found on the Admiralties) in Polynesians that most likely arose in the Bismarck Archipelago, providing the first direct link between northern Island Melanesia and Polynesia. These results significantly advance our understanding of the impact of the Austronesian expansion and human history in the Pacific region.

Patterns of Y-Chromosome Diversity Intersect with the Trans-New Guinea Hypothesis

The island of New Guinea received part of the first human expansion out of Africa (>40,000 years ago), but its human genetic history remains poorly understood. In this study, we examined Y-chromosome diversity in 162 samples from the Bird's Head region of northwest New Guinea (NWNG) and compared the results with previously obtained data from other parts of the island. NWNG harbors a high level of cultural and linguistic diversity and is inhabited by non-Austronesian (i.e., Papuan)-speaking groups as well as harboring most of West New Guinea's (WNG) Austronesian-speaking groups. However, 97.5% of its Y-chromosomes belong to 5 haplogroups that originated in Melanesia; hence, the Y-chromosome diversity of NWNG (and, according to available data, of New Guinea as a whole) essentially reflects a local history. The remaining 2.5% belong to 2 haplogroups (O-M119 and O-M122) of East Asian origin, which were brought to New Guinea by Austronesian-speaking migrants around 3,500 years ago. Thus, the Austronesian expansion had only a small impact on shaping Y-chromosome diversity in NWNG, although the linguistic impact of this expansion to this region was much higher. In contrast, the expansion of Trans-New Guinea (TNG) speakers (non-Austronesian) starting about 6,000-10,000 years ago from the central highlands of what is now Papua New Guinea, presumably in combination with the expansion of agriculture, played a more important role in determining the Y-chromosome diversity of New Guinea. In particular, we identified 2 haplogroups (M-P34 and K-M254) as suggestive markers for the TNG expansion, whereas 2 other haplogroups (C-M38 and K-M9) most likely reflect the earlier local Y-chromosome diversity. We propose that sex-biased differences in the social structure and cultural heritage of the people involved in the Austronesian and the TNG expansions played an important role (among other factors) in shaping the New Guinean Y-chromosome landscape.

Genetic characterization of four Austronesian-speaking populations

Ascertaining the genetic relationships between Austronesian populations is paramount to understanding their dispersal throughout the islands of the Pacific and Indian Oceans. The start of the Austronesian expansion has been dated to approximately 6,000 years ago, and from linguistic and archeological evidence, the origin of this dispersal may have been the island of Formosa. Consequently, the Taiwanese aboriginal populations and their phylogenetic relationship to the Austronesian-speaking groups from Madagascar at the occidental fringes of the expansion are of great interest. In this study, allelic frequencies from six polymorphic point mutation loci were assessed in the Austronesian-speaking populations of Madagascar, the Atayal aborigines of Formosa, and the general populations of Bali and Java. These allelic frequencies were compared and analyzed with the corresponding values from eight other worldwide populations from geographically targeted regions. The group from Madagascar is genetically distinct from their east-African neighbor from Zimbabwe. Our data also indicates that the Ami and the Atayal aborigines in the island of Taiwan, which occupy adjacent territories, differ sharply genetically. Genetic differences were also found between the populations of Bali and Java, belying their geographical proximity. Our results indicate that the east-African population from Madagascar phylogenetically segregates intermediate between mainland east-African and east-Asian groups, corroborating linguistic data indicating the Austronesian influence on this population.

Autosomal STR Variation in Five Austronesian Populations

Human population characteristics at the genetic level are integral to both forensic biology and population genetics. This study evaluates biparental microsatellite markers in five Austronesian-speaking groups to characterize their intra- and interpopulation differences. Genetic diversity was analyzed using 15 short tandem repeat (STR) loci from 338 unrelated individuals from 5 Pacific islands populations, including the aboriginal Ami and Atayal groups from Taiwan, Bali and Java in Indonesia, and the Polynesian islands of Samoa. Allele frequencies from the STR profiles were determined and compared to other geographically targeted worldwide populations procured from recent literature. Hierarchical AMOVA analysis revealed a large number of loci that exhibit significant correspondence to linguistic partitioning among groups of populations. A pronounced divide exists between Samoa and the East (Formosa) and Southeast Asian (Bali and Java) islands. This is clearly illustrated in the topology of the neighbor-joining tree. Phylogenetic analyses also indicate clear distinctions between the Ami and Atayal and between Java and Bali, which belie the respective geographic proximities of the populations in each set. This differentiation is supported by the higher interpopulation variance components of the Austronesian populations compared to other Asian non-Austronesian groups. Our phylogenetic data indicate that, despite their linguistic commonalities, these five groups are genetically distinct. This degree of genetic differentiation justifies the creation of population-specific databases for human identification.

A cautionary tale on ancient migration detection: mitochondrial DNA variation in Santa Cruz Islands, Solomon Islands.

Over the past decade, the origin of the first Malayo-Polynesian settlers of the island Pacific has become a contentious issue in molecular anthropology as well as in archaeology and historical linguistics. Whether the descendants of the ancestral Malayo-Polynesian speakers moved rapidly through Indonesia and Island Melanesia in a few hundred years, or whether they were the product of considerable intermingling within the more westerly part of the latter region, it is widely accepted that they were the first humans to colonize the distant Pacific islands beyond the central Solomon Islands approximately 3,000 years ago. The Santa Cruz Islands in the Eastern Solomons would have most likely been the first in Remote Oceania to be colonized by them. Archaeologically, the first Oceanic Austronesian settlement of this region appears to have been overlain by various later influences from groups farther west in a complex manner. Molecular anthropologists have tended to equate the spread of various Austronesian-speaking groups with a particular mitochondrial variant (a 9-base-pair [bp] deletion with specific D-loop variants). We have shown before that this is an oversimplified picture, and assumed that the Santa Cruz situation, with its series of intrusions, would be informative as to the power of mitochondrial DNA haplotype interpretations. In the Santa Cruz Islands, the 9-bp deletion is associated with a small number of very closely related hypervariable D-loop haplotypes resulting in a star-shaped Bandelt median network, suggesting a recent population expansion. This network is similar to Polynesian median networks. In a pairwise mismatch comparison, the Santa Cruz haplotypes have a bimodal distribution, with the first cluster being composed almost entirely of the 9-bp-deleted haplotypes-again attesting to their recent origins. Conversely, the nondeleted haplogroups bear signatures of more ancient origins within the general region. Therefore, while the profiles of the two sets of haplotypes indicate very distinctive origins in different populations with divergent expansion histories, the sequence of their introduction into the Santa Cruz Islands clearly does not follow simply.

HLA‐DRB1 frequencies of non‐Austronesian‐speaking Gidra in south New Guinea and their genetic affinities with Oceanian populations

DNA typing of HLA-DRB1 genes was conducted for 192 samples from the Gidra who speak one of the non-Austronesian languages and inhabit the southern lowlands of New Guinea. Comparison of the allele frequencies with those of eight other Oceanian populations reveals that the Gidra are genetically closest to the non-Austronesian-speaking Goroka and, next, to the Aboriginal Australian groups, but are remote from the Austronesian-speaking groups in mainland New Guinea and the rest of Melanesia. This finding clearly supports the hypothesis that non-Austronesians and Aboriginal Australians are descendants of the first-stage migrants to Oceania.