Australian Animal Research Articles

An Update on Australian Policy around Lamb Marking with Examination of Potential Drivers. Comment on Johnston et al. How Well Does Australian Animal Welfare Policy Reflect Scientific Evidence: A Case Study Approach Based on Lamb Marking. Animals 2023, 13, 1358.

This commentary provides an update and clarification on the legislative landscape surrounding mulesing in Australia since the publication of the 2023 study, "How Well Does Australian Animal Welfare Policy Reflect Scientific Evidence: A Case Study Approach Based on Lamb Marking". The article explores legislative changes mandating the use of pain relief for mulesing in various states, emphasising Victoria's original role, and highlighting the fragmented state-based legislative approach to animal welfare. It discusses the impact of these legislative changes on industry practices and animal welfare outcomes. The commentary highlights the complexities of policy development in this area, due in part to the diverse and often conflicting interests of stakeholders and the public. It underscores the importance of transparency, stakeholder collaboration, and scientifically informed policymaking to effectively enhance animal welfare standards.

Prevalence of antimicrobial resistance at two private veterinary referral hospitals.

Antimicrobial resistance (AMR) is an emerging human and animal issue. The frequency of resistance to high importance antimicrobials, isolation of microbes of One Health importance and the nature and frequency of multi-drug resistant (MDR) profiles in Australian small animal referral practice have not been described previously. Medical databases of two private small animal referral hospitals in Queensland, Australia were reviewed for culture and susceptibility (C&S) results from 1 January to 31 December 2020. Hospital site (H1 and H2), culture sample, C&S results and MDR were documented for samples from services operating at both locations. There were 631 microbial isolates and 386 susceptibility profiles from 438 samples. The predominant organism was Staphylococcus pseudintermedius at H1 (n = 95) and Escherichia coli at H2 (n = 23). The majority of samples were integumentary (H1 n = 216, H2 n = 75) or urogenital (H1 n = 74, H2 n = 70). MDR isolates were reported at both hospitals, and were significantly more likely at H1 (69/262, 26.3% vs. 12/121, 9.9%; P < 0.001). High levels of AMR including MDR profiles were reported at the two hospitals evaluated, but they had significantly different resistance patterns and microbial profiles. These results highlight the need to practice appropriate antimicrobial stewardship in veterinary medicine, and are supportive for individual hospital surveillance with antibiograms.

Raging with the Machine in the Uncanny Valley: Human–AI Cocreativity in the Eurovision-Themed AI Song Contest

Abstract We report here the processes involved in creating our entry in the 2020 AI Song Contest, “Beautiful the World”; the technical innovations from the project; and the decision-making that divided tasks between human and machine in a way that ensured that the final creation was AI inspired but human created, starting from generated melodies, lyrics, and timbres. Key innovations include the use of lyric stress patterns as queries to a stress-based melody index to a database of generated melodies, and the creation of a novel instrument timbre with differential digital signal processing, trained on Australian animal calls. We reflect on how human–AI cocreativity occurred during the process and how it may develop in the future.

‘We love sharing your land’: Children’s understandings of Acknowledgement to Country practices and Aboriginal knowledges in early learning centres

This paper explores children’s understandings of Acknowledgement to Country practices and Aboriginal knowledges. Guided by the relational lenses of respect, responsibility and reciprocity, we conducted focus groups with children across five Australian early education centres. We found that Acknowledgement practices were evident through recitation of their Acknowledgement to Country, engaging with artefacts, and/or discussion of artworks. Secondly, children demonstrated emerging understandings about place names, the symbolic use of flags for places and people, and Australian plants and animals. Thirdly, Aboriginal cultures as living cultures were evident in temporal discussions about people and culture. Finally, imaginative play implied efforts to make sense of Aboriginal concepts and language. Across the study, children were active in experimenting with ideas in their own meaning making. Acknowledgement to Country was not a moment in the day; rather, it was embedded throughout the day through routines, storytelling, play and creative activities, all designed to foster learning.

Contribution of the veterinary profession to the Australian animal health surveillance system and opportunities for future contributions

Animal health surveillance is crucial for early detection of emergency animal diseases and effective responses. However, surveillance systems are complex and rely on the contributions of many animal health stakeholders. Veterinarians are key stakeholders in this system, given their role and skills in investigating, diagnosing, and reporting notifiable diseases. This study investigated the contribution of the veterinary workforce to the Australian animal health surveillance system and opportunities for future involvement. To achieve the aims of the study, an online cross-sectional survey among the veterinary profession was conducted. Descriptive statistics and regression analyses were used to provide an overview and investigate drivers of attitudes and practices of veterinarians in relation to animal health surveillance. A total of 311 usable responses were obtained, with 191 being from veterinarians who worked in private practice in the previous 12 months. Among private practitioners, 58.6% worked with companion animals, 34.0% were mixed practice veterinarians and 7.3% were equine veterinarians. Over half (56.6%) of all participants considered themselves active participants in the local animal disease management system. The level of confidence in understanding the reporting system and knowing and identifying signs of endemic and exotic diseases was moderate among those working in private practice, with companion animal veterinarians reporting the lowest levels of confidence (p < 0.05). Approximately 40% of veterinarians had taken samples for diagnosis for notifiable diseases in the last year, with just over 20% reporting a notifiable disease. Awareness of and participation in training and surveillance programs for animal diseases by veterinarians was low, with those working in private practice having lower levels of both awareness and participation for most programs. In relation to potential future contribution to the surveillance system, over half of participants reported being interested and available to undertake surveillance work on behalf of the government, with those in mixed practice reporting higher levels of interest (69.6%) compared to those in companion (49.5%) and equine practice (30.8%). However, key challenges identified were related to perceived conflict of interest, and tensions between client needs and government agenda, followed by profitability and suitability of the business. This study provides evidence of a significant existing contribution by the veterinary profession to the surveillance system, and the capacity and willingness to increase this contribution. However, there are gaps in awareness, confidence and participation, as well as financial and veterinary-client relationship challenges that should be considered in any future planning to strengthen the Australian surveillance system.

Declining engagement of veterinary services in Australian animal production systems: why has this occurred and what are the risks?

The provision of veterinary services is essential to deliver animal health and welfare outcomes, but over the last several decades demand for veterinary services in animal production systems has broadly declined in Australia. Reduced demand is closely related to a decline in the size of the production animal veterinary workforce, and there is evidence that the percentage of veterinarians participating in the delivery of veterinary services to animal production systems has lessened. Reduced demand for veterinary services in the production animal sector is likely to be attributed to several factors, including challenges around widespread adoption of preventive veterinary services, improved self-efficacy of producers through advancement of knowledge, and potential concern by producers over the role of veterinarians in production animal systems. Declining veterinary engagement results in increased risk at both the individual farm level (diminished expertise to deliver reactive and proactive veterinary services) and at a population level (increased biosecurity risk and risk to social licence to operate). The current environment and the community trust in the profession should be seen as an opportunity to develop and implement a strategy to halt and reverse the decline in demand for production animal veterinary services. Such a strategy will require significant and sustained collaboration between the veterinary profession, Industry and government.

Increasing the productive potential of large and small cattle breed resources in the South of Russia based on modern breeding methods

The results of the genetic characteristics research of large and small cattle populations grown in the Southern Russia are stated. Breed-specific DNA patterns have been identified in three of the most common cattle breeds of meat productivity in arid conditions. The milk productivity of Holstein cows of different breeding lines has been studied. It was found that increasing the frequency of animals of Vis Back Ideal and Reflection Sovering lines in the parent pairs could be able to increase the level of milk productivity of offspring and improve the quality indicators of dairy raw materials. The adaptive abilities and milk productivity of imported foreign Holstein cows depending on their origin were estimated. In comparison with Australian and Danish Holstein cows, American and German analogues have a higher level of milk productivity. However Australian and Danish animals have a more flexible adaptive capacity. As a result of the comparative study of the economic and biological characteristics of two generations of Aberdeen-Angus cattle (parental foreign generation, and the first generation - obtained under breeding conditions in Southern Russia), tendencies to increase productive values are revealed. The features of the meat productivity formation in Kalmyk steers different exterior and constitutional types are investigated. It was found that the Kalmyk steers of the tall body type have a higher meat productivity compared with their analogues of medium and compact body types. However, the beef of compact steers has a higher fat percentage, more attractive taste characteristics, culinary and technological indices. The features of polymorphism of CAST, GH, GDF9, CLPG, FABP4 and MC4R genes in populations of sheep of different breeds grown in the Republic of Kalmykia, Volgograd and Rostov regions, Kabardino-Balkaria Republic were also studied. As a promising method of early diagnosis of presence economically useful traits in sheep during ontogenesis, the effectiveness of DNA analysis in young lambs and the further selection of animals with desirable genotypes according to these genes were justified.

Few havens for threatened Australian animal taxa that are highly susceptible to introduced and problematic native species

Few havens for threatened Australian animal taxa that are highly susceptible to introduced and problematic native species

The economic impacts of a hypothetical foot and mouth disease outbreak in Australia

AbstractThis study uses a multicountry, dynamic, quarterly CGE model, GlobeTERM, to estimate the economic impacts of a hypothetical foot and mouth disease outbreak in Australia. The national welfare losses arising from the outbreak depend mostly on the duration of trade sanctions by importers of Australian animal products. If an outbreak is contained within several months, and trade sanctions are dropped within a year of the outbreak, the net present value of Australia's welfare losses may be around AUS$10 billion. If all importers restore Australian access within a year, other than China–Hong Kong, which delays by 5 years, welfare losses are around AUS$21 billion. In a less likely scenario, in which trade sanctions persist in all trading partners for 5 years after the disease has been eradicated, contrary to international guidelines, welfare losses may exceed AUS$85 billion. Trading partners also suffer welfare losses due to trade sanctions. These losses are large enough to imply, from a global perspective, that a shift towards vaccinate‐to‐live policies combined with global efforts to eradicate the disease may be cost‐effective.

Biogeographic distribution and molecular epidemiology of Clostridioides (Clostridium) difficile in Western Australian soils.

Clostridioides (Clostridium) difficile is a leading cause of infectious diarrhea in humans and production animals and can be found in a variety of environmental sources. The prevalence and diversity of multi-locus sequence type clade 5 strains of C. difficile in Australian production animals suggest Australia might be the ancestral home of this lineage of One Health importance. To better understand the role of the environment in the colonization of humans and animals in Australia, it is important to investigate these endemic sources. This study describes the prevalence, molecular epidemiology, and biogeographic distribution of C. difficile in soils of Western Australia. A total of 321 soil samples from remote geographical locations across the eight health regions of Western Australia were screened for C. difficile and isolates characterized by PCR ribotyping and toxin gene profiling. C. difficile was isolated from 31.15% of samples, with the highest prevalence in the Perth Metropolitan Health Region (49.25%, n = 33/67). Overall, 52 different strains [PCR ribotypes (RTs)] were identified, with 14 being novel, and 38% (38/100) of isolates being toxigenic, the most common of which was RT014/020. Five unique novel isolates showed characteristics similar to C. difficile clade 5. This is the first study of C. difficile isolated from soils in Australia. The high prevalence and heterogeneity of C. difficile strains recovered suggest that soils play a role in the survival and environmental dissemination of this organism, and potentially its transmission among native wildlife and production animals, and in community and hospital settings.IMPORTANCEClostridium difficile is a pathogen of One Health importance. To better understand the role of the environment in human and animal colonization/infection, it is critical that autochthonous reservoirs/sources of C. difficile be investigated. This is the first study of C. difficile isolated from soils of Western Australia (WA). Here, the ecology of C. difficile in WA is described by examining the geographic distribution, molecular epidemiology, and diversity of C. difficile isolated from soils across WA.

Collections from Colonial Australia in Berlin's Museum für Naturkunde and the Challenges of Data Accessibility

German-speaking naturalists working in southeastern Australia in the mid-19th century relied heavily on the expertise of First Nations intermediaries who acted as guides, collectors, traders and translators (Clarke 2008, Olsen and Russell 2019). Many of these naturalists went to Australia because of the research opportunities offered by the British Empire at a time when the German nation states did not have colonies of their own. Others sought to escape political upheaval at home. They were welcome employees for colonial government agencies due to their training in the emerging research-oriented natural sciences that the reformed German universities offered at a time when British universities were still providing a broad general education (Home 1995, Kirchberger 2000). Wilhelm von Blandowski (1822–1878 ) and Gerard Krefft (1830–1881 ), who both worked in colonial Victoria and New South Wales, are among this group. Throughout their work, they corresponded extensively with naturalists in Berlin, exchanging specimens and ideas. But the preserved Australian animals, plants and rock samples, as well as the written and drawn records of animals and landscapes now held at the Museum für Naturkunde Berlin (MfN), are much more than objects of scientific interest. They also contain information about Australia's First Nations. The collections provide evidence of their role in collecting as well as their knowledge of the natural world, which has long been overlooked and, at least in part deliberately, made invisible by Western knowledge systems (e.g., Das and Lowe 2018, Ashby 2020). People data have been recognised as crucial for linking such collection objects with expeditions, publications, archival material and correspondence (Groom et al. 2020, Groom et al. 2022). It can thus potentially help reconstruct invisibilized Indigenous histories and knowledge. However, while the MfN keeps information about European collectors and other non-indigenous agents associated with their specimens in internal catalogues, databases and wikis, Indigenous actors remain largely absent from these repositories, which reproduce the colonial archive 'along the archival grain' (Stoler 2009). With this in mind, we discuss in our presentation the complexities of using persistent identifiers and tools, such as Wikidata, to improve the integration and linkage of people data in the work currently being undertaken by the MfN and the Berlin's Australian Archive project to digitise and make accessible the museum’s collections. Drawing upon the guidance provided by the FAIR*1 and CARE*2 principles for data (Wilkinson et al. 2016, Carroll et al. 2020), and learning from the 2012 ATSILIRN Protocols for Libraries, Archives and Information Services*3, the 2019 Tandanya Adelaide Declaration and the 2020 AIATSIS Code of Ethics*4, we address the potential of these efforts in terms of collection accessibility, and also highlight the challenges and limitations of this approach in the context of colonial collections.

Cross-sectional evaluation of a large-scale antimicrobial stewardship trial in Australian companion animal practices.

Antimicrobial stewardship programmes (ASPs) are critically important for improving the appropriate use of antimicrobial agents in veterinary practice. One of three ASPs was implemented in 135 Australian general veterinary practices in 2018-2020. The ASP interventions and the perceived impact they had on antimicrobial prescribing were assessed by the veterinarians working in these veterinary practices. An online survey was distributed to all 520 veterinarians working in the trial practices and 267 responses were analysed. Most veterinarians (174/267, 65%) thought they had an ASP at their clinic and most respondents who said that they were aware that they had an ASP at their clinic indicated that they had changed the way they prescribed antimicrobials because of the trial (125/170, 74%). Antimicrobial prescribing guidelines, the traffic light system for indicating antimicrobial importance, delayed prescribing and antimicrobial stewardship champions were reported to have had the biggest impact. All practices in the trial belong to a single corporate group, which may impact the external validity of these results when applied to general small animal practice. Antimicrobial stewardship has a positive impact on antimicrobial prescribing in veterinary medicine and future interventions should focus on the implementation of the effective interventions identified in this study.

A Novel Marine Mammal Coxiella burnetii-Genome Sequencing Identifies a New Genotype with Potential Virulence.

The obligate intracellular bacterial pathogen Coxiella burnetii has been identified in a few species of marine mammals, some of which are showing population declines. It has been hypothesized that C. burnetii in marine mammals is a distinct genotype that varies significantly from the typical terrestrial genotypes. It appears to lack an IS1111. Isolates originating from Australian marine animals have a distinctly non-Australian profile of multiple-locus variable-number tandem-repeat analysis (MLVA). Extracted Coxiella DNA of Australian fur seal placental origin was sequenced using the Novaseq platform. Illumina 150 bp paired-end reads were filtered and trimmed with Trimgalore. The microbial community present in the sequenced genome was evaluated with Kraken and Bracken software using the NCBI database. A phylogenetic analysis was performed using 1131 core genes. Core genes were identified using Panaroo and inputted into Iqtree to determine the maximum-likelihood tree. A second phylogenetic tree was created using Rickettsiella grylii and using seven housekeeping genes. Results were compared with the C. burnetii Nine Mile RSA439 virulent genome. This new Australian marine mammal isolate of Coxiella (PG457) appears to be a novel genotype that lacks IS1111 and has a distinct MLVA signature (ms26, ms27, ms28, ms30, and ms31). The presence of genes for multiple virulence factors appears to give this genotype sufficient pathogenicity for it to be considered a possible causative agent of abortion in Australian fur seals as well as a potential zoonotic risk.

How Well Does Australian Animal Welfare Policy Reflect Scientific Evidence: A Case Study Approach Based on Lamb Marking.

The development and substance of animal welfare policy is subject to a range of social, cultural, economic, and scientific influences that commonly vary within and between countries. Discrepancies in policy can create confusion and mistrust among stakeholders and consumers and limit the ability to create a uniform minimum level of requirements to safeguard animal welfare, as well as create a level 'playing field' for farmers when trading with other jurisdictions. The livestock sector is receiving growing scrutiny globally for real and perceived violations of animal welfare, for example, the practice of mulesing in Australia. This article explores animal welfare legislation within Australia and how it reflects the scientific evidence surrounding routine husbandry practices in sheep, including tail docking, castration, and mulesing. While there is some variation between state and territory legislation, the most notable concern is the lack of enforceable recommendations surrounding the evidence-based use of analgesia and anaesthesia for painful husbandry procedures. The age at which these procedures are recommended to be performed is relatively consistent across Australian jurisdictions, but there is a marked difference compared to international legislation. The global context of animal welfare legislation, public perception, and producer perception of these procedures are also discussed, highlighting the difficulty of creating robust animal welfare legislation that promotes a good standard of welfare that is respected worldwide whilst being practical in an Australian setting given our unique geography and climatic conditions.

A Typology of Australian Animal Sentience Recognition Provisions — Enacted and Proposed

Australia appears to be following the trend in mainly Western countries of recognising animal sentience in the law. This article sets out a typology of animal sentience recognition provisions that have been enacted, or have been proposed, in Australian jurisdictions to date. These include provisions or proposed provisions located in statutory objects, statutory principles, statutory definitions and a treaty. Depending on legislative context, these provisions, and proposed provisions (if enacted), may have different legal consequences. The trend towards legally recognising animal sentience may also signal further positive legal reforms for animals in the future.

Investigating the Source of Salmonella Agona Contamination in Australian Feed Mills Using Core Genome Phylogenetic Analysis

Salmonella enterica serovar Agona is commonly detected in raw animal feed components during routine microbial monitoring of Australian commercial animal feed mills. We hypothesized that Salmonella-contaminated raw feed components originate at the rendering or oil seed crushing plant and are distributed to mills in different locations. Our objective was to investigate the source of Salmonella Agona contaminated raw feed components. Whole genome sequences of 37 Salmonella Agona isolates, 36 from raw feed components and 1 from finished feed, collected from 10 Australian feed mills located in 4 Australian states, were compared using core genome phylogenetic analysis. After DNA extraction and de novo draft assembly of the paired reads, the draft genomes were aligned using conserved signature indel phylogeny against a reference genome for Salmonella Agona, to identify single nucleotide polymorphisms in the core genome. Five distinct clades corresponding to the five different suppliers of Salmonella Agona-contaminated raw feed components were identified in the resulting phylogenetic tree. The results also provided evidence of cross-transference of Salmonella Agona between canola meal, meat meal, and finished feed within a mill. Core genome phylogenetic analysis facilitated tracing the source of Salmonella contamination in feed mills.

Attitudes and Beliefs of a Sample of Australian Dog and Cat Owners towards Pet Confinement.

Most cats and dogs entering Australian animal shelters and municipal facilities are classed as strays, typically from low socio-economic areas. Contemporary practices increasingly focus on proactively reducing the number of stray animals, which requires further understanding of factors associated with straying animals, including pet confinement. Australian cat and dog owners (n = 2103) were surveyed to investigate attitudes towards four types of pet confinement and how these were influenced by social norms, demographics and concerns about pet quality of life and potential wildlife predation. Dog owners showed the strongest support for confining dogs to the owners' property whenever unsupervised (98% agreement) and less support for confining dogs inside the house at night (54% agreement), and only 23% believed dogs had a negative impact on wildlife. Cat owners showed the strongest support for confining cats inside the house at night (89% agreement). Cat owners' non-supportive attitudes towards cat confinement were partly because of higher concern for cat quality of life and lower concern about their cats' predation behaviours, compared to non-cat owners. The findings provide valuable information to inform more effective strategies to reduce stray animals which would reduce shelter admissions, euthanasia, costs, nuisance issues, potential wildlife predation and negative mental health impacts of euthanasia on staff. Strategies to reduce strays include assisting low-income pet owners to install effective fencing and programs to increase identification. Informing cat owners about bedtime feeding is recommended to assist with night containment, and providing high-intensity free sterilization of owned and semi-owned cats targeted to areas of high cat impoundments is also recommended.

Phantom in the Landscape

The body of artworks entitled The Golden Feral Trail emerges out of my journeys tracing the relationship between South Asia and Western Australia (WA). Linked by trade and migration flows since the early 1800s, the trail unfolds along the Gold Rush in WA and the detritus it has left behind. Drawing upon visits to cemeteries, abandoned graves, deserted mining pits, ghost towns as well as on institutional archives, oral histories and personal photo albums in WA, I collected traces of the South Asian cameleers and traders, referred to as ‘Afghans’. A shorthand for very diverse nomadic sects found in British records, together with local Wongutha ‘Afghans’ led British explorers in their gold exploration missions into the outback. This article argues that much has gone feral in the contemporary Australian landscape, including seeds, animals, abandoned mines and mining towns. The Golden Feral Trail – a history of nomadic economy, loss and erasure – runs beneath the Australian red soil. What is left on the surface is landscape phantoms.

Lights at the end of the tunnel: The incidence and characteristics of recovery for Australian threatened animals

Recovery of threatened species is a widely recognised conservation goal. We assess the incidence and characteristics of recovery for threatened Australian animals from the establishment of Australia's national environmental legislation in 2000 to 2022. Formal de-listings have been few, and mostly not indicative of actual recovery. However, we assessed that 29 taxa (1 fish, 4 frogs, 1 reptile, 8 birds and 15 mammals), representing 6.5 % of the 446 species that we consider were justifiably listed as threatened, have recovered over this period such that they no longer meet the eligibility criteria for listing as threatened.Most of the recovered species are mammals whose previous decline was due to introduced predators. Their recovery has been enabled by sustained management actions (establishment of predator-free havens, translocations and predator control). The lack of recovery of invertebrates is possibly because these have received little conservation investment. The limited recovery of fish is due to limited capacity for abating the threats of introduced fish predators and of exploitation and degradation of aquatic systems. Species threatened by habitat loss and degradation, fire and climate change are under-represented in recoveries.De-listing of the taxa that we assess here to have recovered would provide a tangible recognition and indicator of conservation success and help maintain the integrity of the threatened species list. However, most of the recovered species would rapidly become eligible for re-listing should their conservation management be withdrawn. Although there is a prevalent trend for decline of Australia's threatened species, these recoveries merit recognition.

Different motivations for volunteering are associated with different outcomes for Australian animal welfare volunteers: a mixed-methods analysis

ABSTRACT Objective Inspired by the important role volunteers play in Australian society, the current study examined the relationship between different motivations for volunteering and different outcomes experienced by animal welfare volunteers. Method An anonymous, self-report questionnaire was completed online by 278 volunteers (84.53% female; M age = 45.19 years, SD = 18.13) of a large state-wide animal welfare organisation. A latent profile analysis was conducted with data collected using the Volunteer Functions Inventory (VFI), and interpreted alongside a thematic analysis of qualitative responses. Results Three groups of participants were identified, characterised by different prominent motivations to volunteer: personal values (values-driven group); personal values and personal growth (personal-gain group); and personal values, personal growth, and career-related factors (experience-oriented group). Values-driven volunteers experienced both pleasure and stress from the enactment of values-driven behaviour, and experienced increased opportunity for socialisation. Personal-gain volunteers experienced increased opportunity for socialisation and increased sense of purpose. Experience-oriented volunteers derived pleasure from enacting personal values, and increased opportunity to gain experience in animal-care. Conclusions Motivations for volunteering are complex and multifaceted. The current findings highlight how motivationally congruent volunteering behaviours can be associated with a range of positive outcomes for volunteers.