Aureus Bacteria Research Articles

Evaluation of Antibacterial Activity of Bismuth Ferrites Nanoparticles in the Inhibition of E. Coli and S. Aureus Bacteria.

In this work, bismuth ferrites (BFO) nanoparticles were produced in the form of using sol-gel technique, followed by annealing in a tube furnace in temperatures from 400 °C to 650 °C. X-ray diffraction (XRD) results showed the formation of small sizes nanoparticles (NPs) with high purity. Structural analysis displayed that annealing at 600 °C could make BFO NPs be fitted to rhombohedral space group (R3c), with small quantity of spurious phases. The sizes of the BFO nanoparticles determined by transmission electron microscopy (HRTEM) are between 50 to 100 nm. To evaluate the efficiency of BFO in antimicrobial susceptibility tests, the nanoparticles were dispersed through cand tested agar diffusion method and dilution in a 96 well plate using a Gram-positive strains (Staphylococcus aureus) and Gram negative strain (Escherichia coli). The antibacterial activity of the BFO NPs was tested at concentrations of 2 mg/mL with MIC greater than 60 μg/mL for both bacteria.

Oral delivery of Sunitinib malate using carboxymethyl cellulose/poly(acrylic acid-itaconic acid)/Cloisite 30B nanocomposite hydrogel as a pH-responsive carrier.

This work aimed to fabricate a Cloisite 30B-incorporated carboxymethyl cellulose graft copolymer of acrylic acid and itaconic acid hydrogel (Hyd) via a free radical polymerization method for controlled release of Sunitinib malate anticancer drug. The synthesized samples were characterized by FTIR, XRD, TEM, and SEM-dot mapping analyses. The encapsulation efficiency of Hyd and Hyd/Cloisite 30B (6 wt%) was 81 and 93%, respectively, showing the effectiveness of Cloisite 30B in drug loading. An in vitro drug release study showed that drug release from all samples in a buffer solution with pH 7.4 was higher than in a buffer solution with pH 5.5. During 240min, the cumulative drug release from Hyd/Cloisite 30B (94.97% at pH 7.4) is lower than Hyd (53.71% at pH 7.4). Also, drug-loaded Hyd/Cloisite 30B (6 wt%) demonstrated better antibacterial activity towards S. Aureus bacteria and E. Coli. High anticancer activity of Hyd/Cloisite 30B against MCF-7 human breast cancer cells was shown by the MTT assay, with a MCF-7 cell viability of 23.82 ± 1.23% after 72-hour incubation. Our results suggest that Hyd/Cloisite 30B could be used as a pH-controlled carrier to deliver anticancer Sunitinib malate.

Education on acne and cosmetic knowledge for adolescents at SMK Rise Kedawung

Acne occurs due to the presence of S. Aureus bacteria, when the oil glands in the skin are too active, acne usually appears on the surface of the skin of the face, neck, chest, and back. Therefore, antibacterials are needed to overcome this, which can be obtained from chemicals or traditional plants. The aim of this community service program is to provide education to students at Rise Kedawung Vocational School about acne on teenage skin and training to check cosmetics registration using the BPOM Mobile application. This activity is carried out by providing material about acne starting from understanding acne, the causes of acne, traditional plants that have antibacterial activity, chemicals that have antibacterial activity, and how to check BPOM registration. The pre-test and post-test results showed an increase in participants' understanding regarding acne, acne medication, and checking cosmetic registration from 87% to 96%.

Antibacterial activities of three species of mangrove leaves extract against Staphylococcus aureus and Escherichia coli

The increasing incidence of infections and drug resistance has led scientists to seek approaches towards medicinal plants that are potentially effective against many microorganisms. Therefore, this study was planned to assess the antibacterial activity of 3 mangrove species (Scyphipora hydrophylaceae C.F.Gaertn., Lumnitzera littorea (Jack) Voigt and Avicennia alba Blume) against Staphylococcus aureus and Escherichia coli bacteria. This study used the disc diffusion method to measure the diameter of inhibition at several concentrations of mangrove leaf ethanol extracts, namely 15%, 10%, 5%, 2.5%, 1.25% and 0.625% b/v as well as positive control cefadroxil for bacteria and DMSO as negative control. The test results showed that ethanol extracts of mangosteen leave Scyphipora hydrophylaceae C.F.Gaertn and Lumnitzera littorea (Jack) Voigt had a solid response to Escherichia coli bacteria and moderate potential against S. Aureus bacteria—no antibacterial activity against both test bacteria for Avicennia alba mangrove. This study concludes that Scyphipora hydrophylaceae C.F.Gaertn and Lumnitzera littorea (Jack) Voigt have potent antibacterial activity against Escherichia coli, but Avicennia alba has no antibacterial activity.

The Effect of Titanium Dioxide Nanoparticle Composites as an Antibacterial in Synthesizing Polyurethane Biopolymers

Abstract. In the health sector, the use of polyurethane (PU) as a basic material for the manufacture of medical devices also creates problems related to local and systemic infections. One of the most appropriate ways to overcome this problem is to add titanium dioxide (TiO2) nanoparticles to the urethane polymer to produce a biodegradable polymer and bacterial decontamination. In synthesizing decontaminating polyurethane bacteria, several characterization techniques were carried out, including polymer test, namely strain and stress, functional group analysis using Fourier Transform Infra-Red (FTIR), and antibacterial test. Based on the results of the FTIR test analysis, shows a change in the functional group. At wave number 1724.36 cm-1, the N-H functional group appears, this absorption is the absorption of the urethane group and TiO2 is in the range of 513.07 cm-1. Mechanical properties test showed strain (28.92 - 21.88% GL) and young modulus at intervals (5,484-3,268 MPa). and the antibacterial test showed that the inhibitory power of test samples A1 and A4 with resistance diameters of 8mm and 8mm proved to be very effective in killing E.Coli bacteria while A1¹, A2¹, A3¹, and A4 killed could not kill S. Aureus bacteria inhibitors. The characterization results show that the polyurethane biopolymer can be used as a medical device with bacterial decontamination properties.

Extension of Raw Cow Milk Shelf Life by Microplasma Discharge

Cow's milk, the universal nutrient, is being stored and supplied, which seeks proper preservation. The prevalent milk preservation procedure of refrigeration, is effective only for two days, and after which, it starts to contaminate due to the growth of various milk-laden bacteria. This bacterial overload has to be inactivated properly to increase its shelf life, and is been achieved effectively using microplasma, a single-step, cost-effective and chemical-free process. Raw milk was treated for 5, 10, and 13 seconds in microplasma discharge. After 13 seconds of microplasma treatment, E. Coli, Pseudomonas, and S. Aureus bacteria got reduced at a respective rate of 89.93, 84.55, and 94.19% for in raw milk. The reactive species formed during microplasma discharge disrupts the structural integrity of bacterial cells and inactivates it, thereby enhancing the milk shelf life. Treated samples remained in good condition for 8 days. Thus, microplasma discharge increases the shelf life of milk by quickly inactivating the bacterial load.

Synthesis of SnO2 nanoparticles coated ZnO–g–C3N4 composite nanostructures with novel antibacterial activity

Abstract In this work, composite nanostructures (CNs) of SnO2–ZnO–g–C3N4 have been prepared by a soft chemical method and investigated for their potential application in photocatalytic remediation of organics in water and antibacterial agents. Structural study revealed the presence of three phases related to hexagonal wurtzite phase of ZnO, tetragonal phase of SnO2 and monoclinic phase of g–C3N4 having nanocrystalline nature which confirms the formation of CNs. Formation of nanoscale morphology along with elemental composition of the CNs have been validated through scanning electron microscopy (SEM) coupled with energy dispersive X-ray (EDX) spectroscopy. The presence of only A 1g mode of SnO2 in Raman spectrum of ternary CNs suggested that SnO2 nanoparticles have been coated on the surfaces of ZnO and g–C3N4 which is also evident from SEM results. SnO2–ZnO–g–C3N4 CNs have shown much higher photocatalytic degradation efficiency and produced 7 mm greater zone of inhibition (ZOI) against Gram-positive S. Aureus bacteria as compared to pure ZnO which is quite significant result when compared to previously reported results for SnO2–ZnO CNs. These synthesized CNs may have potential uses in healthcare technology and treatment of organics in water.

In vitro antibacterial and cytotoxicity assessment of magnetron sputtered Ti1.5ZrTa0.5Nb0.5W0.5 refractory high-entropy alloy doped with Ag nanoparticles

The aim of the current study is to develop and evaluate antibacterial and biocompatible refractory high-entropy alloy (RHEAs) film using RF magnetron sputtering technique. For this purpose, Ti1.5ZrTa0.5Nb0.5W0.5 RHEA film and its Ag nanoparticle doped analog (with an Ag content of 9 atomic %) were deposited on Ti6Al4V substrate. The microstructural characterization revealed the homogenous distribution of the constituent elements and amorphous structure of the deposited films. The antibacterial activity of Ag nanoparticles doped RHEA film was compared with that of undoped film and uncoated Ti6Al4V. The results indicated that doping Ag nanoparticles reduced the colony forming unit of P. Aeruginosa and S. Aureus bacteria by 98.5% and 90.9%, respectively. In addition, healthy C2C12 mouse muscle myoblast cells adhered and proliferated perfectly on the surface of antibacterial Ag nanoparticles doped RHEA film with no indication of toxic effect, demonstrating promising biocompatibility in addition to its strong antibacterial property.

Biocompatibility and antibacterial behavior of electrochemically deposited Hydroxyapatite/ZnO porous nanocomposite on NiTi biomedical alloy

In this article, Hydroxyapatite (HA) and its composite with ZnO nanoparticles were electrochemically deposited on NiTi superelastic alloy to evaluate the effect of nanoparticles on its various properties. Morphological observations revealed that the addition of 400 mg/L ZnO nanoparticles created an HA layer with a porous network structure. FTIR and GIXRD results pointed out that the nanoparticles contributed to pure HA synthesis. Given AFM topography parameters, a significant rise was detected in roughness values as ZnO entered the HA matrix. The rough surface with a larger volume of porosity improved the wettability of the composite coating by reducing the contact angle to approximately half of its initial value (14.57°). Furthermore, ZnO incorporation proved to be quite helpful in contact with living species. Cell culture assessments disclosed the considerable impact of ZnO nanoparticles on MG-63 osteoblast-like cells’ attachment and proliferation on the composite coating. They also improved cell viability reported by the MTT assay. The antibacterial resistance of HA and HA/ZnO coatings was investigated against E. Coli and S. Aureus bacteria colonies by the disk diffusion method. For the first time, the pure HA matrix showed antibacterial activity, though insignificant. The addition of ZnO nanoparticles promoted this behavior which was more vigorous in the case of S. Aureus bacteria.

Statistical Study of Nonthermal Plasma-Assisted ZnO Coating of Cotton Fabric through Ultrasonic-Assisted Green Synthesis for Improved Self-Cleaning and Antimicrobial Properties.

Nonthermal plasma processing is a dry, environment-friendly and chemical-free method of improving the wettability, adhesion, self-cleaning and dying quality of fabrics without affecting their bulk properties. This study presents a green synthesis and coating method for the immobilization of nanoparticles of ZnO on the nonthermal plasma functionalized cotton fabric. The self-cleaning activity of ZnO-coated cotton was then optimized statistically. The ultraviolet protection and antimicrobial activity of the optimized and a control sample were also elaborated in this study. Psidium guajava Linn (guava) plant extract and zinc chloride were used in the ultrasonic biosynthesis of ZnO nanoparticles and concurrent immobilization over plasma functionalized cotton. Sodium hydroxide was used as a reaction accelerator. Statistical complete composite design (CCD) based on the amount of ZnCl2, NaOH and plasma exposure time was used to optimize the role of input parameters on the self-cleaning ability of the coated cotton. Methylene blue in water was used as a sample pollutant in the self-cleaning study. The ZnO-coated cotton showed notably high self-cleaning activity of 94% and a UV protection factor of 69.87. The antimicrobial activity against E. Coli and S. Aureus bacteria was also appreciably high compared to the control.

The inhibitory effect of aqueous and alcoholic extract of red pepper on some isolated pathogenic bacteria from different areas of human body

In most underdeveloped nations, traditional medicine, including herbal treatment, is still widely used. Due to the growth of antibiotic resistance, this study aims to use pepper as an anti-bacterial as alternative to antibiotics. Pepper is one of the most important plants used as a medicine for a long time in various countries and civilizations. This study aims to use pepper as an anti-bacterial in alternative to antibiotics. The current study included the inhibitory efficacy of aqueous and alcoholic red pepper extract on seven bacterial isolates: -Staphylococcus aureus, Streptococcus spp, Escherichia coli, Klebseilla spp, Salmonella spp, Pseudomonas aeruginosa, Proteus spp. These isolates were isolated from different pathologies and regions, and they were diagnosed according to the site of infection. Several antibiotics were also used as a standard control sample for germs. This study shows that the highest inhibitory Effect against E. coli bacterium, as the average inhibition diameter was about 16.5 mm, and it is an excellent inhibitory when compared to the antagonist's gentamicin and nitrofurantoin as it showed good inhibitory efficacy against the bacteria such as S. aureus P. aeruginosa, and Salmonella spp. While the effect on Klebsiella was equal, on the other hand, the aqueous extract did not show any efficacy against Proteus spp, as was shown in the results. The results also showed that Staph. Aureus bacteria were the most affected by the alcohol extract of the red pepper as it showed a high inhibition zone compared with the control sample tetracycline and nitrofurantoin. The plant's aqueous and alcoholic red pepper extracts were effective against the tested bacterial isolates. The plant's aqueous and alcoholic red pepper extract has good inhibitory efficacy against the studied bacterial isolates.

Psidium Guajava L. Extract Against Minimum Inhibitory Concentration of Staphylococcus Aureus

The aim of this research was to evaluate the effects on the growth of Staphylococcus Aureus bacteria in vitro of antibacterial activity of guayava leaf extract (Psidium guajava L.) and guavava leaf extract impact on the growth of staphylococcus Aureus bacteria at different laboratory concentrations. In this experiment, S. Aureus bacteria are being treated to guava leaf extract to identify the lowest inhibitory power for well techniques, and the results have been recorded. The Guava leaf extract (Psidium Guajava L) against Staphylococcus aureus was found to have an inhibitory zone diameter of mm in the absence of tetracycline, and DMSO as a negative control. Findings were observed using the disk diffusion technique or paper disc with concentrations of 1 percent, 3 percent, 5 percent, and 7 percent. Saponins, saponins, alkaloids, and flavonoids found in guava leaves are responsible for their antibacterial effects. Saponins have the potential to disturb the integrity of bacterial cell membranes. This results in bacterial cell membrane destruction, releasing essential components from the cell

Uji Efektivitas Antibakteri Handsanitizer Minyak Atsiri Kalamansi (Citrofortunella Microcarpa) Terhadap pertumbuhan Escherichia coli dan Staphyllococcus Aureus Secara In Vitro

This study aims to determine the antibacterial activity, effective concentration, and the effect of increasing the concentration of Kalamansi orange essential oil in Handsanitizer gel on the inhibition of growth of Eschericia coli and Staphyllococus Aureus bacteria. Testing for antibacterial activity using the agar cakram method. The parameter that is measured is the diameter of the resistivity formed around the disc paper. Antibacterial activity test results were analyzed by descriptive method. The results showed that the concentration of essential oil in the Handsanitizer gel was 1%; 2%; and 4% have provided inhibitory activity for the growth of the tested bacteria. Effective concentration that can inhibit Eschericia coli and Staphyllococus Aureus bacteria at a concentration of 4%. The increase in the concentration of kalamansi orange essential oil showed the wider the diameter of the inhibition zone for bacterial growth. The inhibition that occurs in the Eschericia coli and Staphyllococus Aureus bacteria, proves that the Gel Handsanitizer Preparation Kalamansi orange essential oil contains limonene active compounds which are antibacterial. From the research that has been done, the following conclusions can be drawn: The availability of Kalamansi orange essential oil gel Handsanitizer with a concentration of 1%, 2% and 4% can inhibit the activity of the bacteria Eschericia coli and Staphyllococus Aureus in vitro. With the higher the concentration of kalamansi orange essential oil, the stronger the antibacterial inhibition is produced so that the handsanitizer with a concentration of 4% has the strongest antibacterial power against Eschericia coli and Staphyllococus Aureus bacteria.

Superparamagnetic Manganese Ferrite and Strontium Bioactive Glass Nanocomposites: Enhanced Biocompatibility and Antimicrobial Properties for Hyperthermia Application

Herein, the strontium bioactive glass with ferrite composites is studied for the application of biocompatibility and magnetic hyperthermia. The bioactive glass SrBG (46.1SiO2–21.9CaO–24.4Na2O–2.6P2O5–5SrO) prepared by the melt quench technique at 1400 °C and manganese ferrite (MnFe2O4) prepared by the modified microwave reflux oven are mixed together and sintered at 400 °C for 6 h for making composites. The in vitro bioactivity of these composites is observed by immersing in simulated body fluid (SBF). The growth of the precipitated hydroxyapatite phase observed after SBF treatment is confirmed by the X‐ray diffractometer (XRD), Fourier transform infrared (FTIR) spectrometer, and scanning electron microscope (SEM) results. The in vitro cell viability assessment of these composites with MG‐63 cell lines indicates that the composites are biocompatible in nature. Furthermore, these composites show the antibacterial effect on the E. coli and S. Aureus bacteria cells. The room temperature superparamagnetic behavior accompanied by the heating ability in these composites proves their potential role for hyperthermia applications.

Uji Ekstrak Daun Maja (Aegle marmelos L.) terhadap Pertumbuhan Bakteri Escherichia coli dan Staphylococcus aureus

Aegle marmelos L. is one of the tropical plants that has many benefits and also has the potential to be used as herbal medicines. It caused in some areas in Indonesia, A. marmelos have been widely used in traditional medicine. This research was a qualitatively descriptive study to see the effectiveness of A. marmelos leaf extract in inhibiting the growth of Escherichia coli and Staphylococcus aureus bacteria. Stages of A. marmelos leaf extract inhibition test on bacterial growth included the stages: extraction by maceration method, phytochemical test, chromatography test, anti-microbial test. Data were analyzed qualitatively. The results showed that A. marmelos leaf extract was more effective in inhibiting the growth of E. coli bacteria than in S. Aureus bacteria. This can be information that Maja leaf extract can be used as a phytopharmaca material for diseases caused by E.coli

Chitosan/PEG/MWCNT/Iodine composite membrane with enhanced antibacterial properties for dye wastewater treatment

Bacterial adherence on solid surface causes biofilm and biofouling where it remains as a critical issue in ultrafiltration membrane technology. Herein, a promising strategy to combat this problem via surface functionalization with iodine on chitosan biopolymeric composite membranes is reported. The Chitosan/PEG/MWCNT/Iodine membranes (PEG: polyethylene glycol, MWCNT: multi-walled carbon nanotubes) were prepared through three simple steps, namely mixing of membrane precursor solutions, membrane casting and incorporation of iodine via phase inversion technique. The microscopy and spectroscopy results revealed that enhanced interactions of iodine (0.10-0.31 wt.%) with membrane matrix via hydrogen bonding networks had successfully improved the membrane properties and performance. Furthermore, significant change in morphological property of membranes (e.g. surface roughness) upon the addition of iodine led to the enhancement of membrane hydrophilicity (contact angle from 70.3° up to 59.3°), porosity (average pore radius from 13.3 nm up to 23.2 nm), and mechanical strength (Young modulus from 6138.6 MPa up to 7824.9 MPa). The composite membranes exhibited superior antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) even at very low iodine concentration (0.31 wt.%) with bacteria killing ratio of up to 99.2% and 100% for E. Coli and S. Aureus bacteria, respectively. The composite membranes also demonstrated improved permeability (flux up to 212.8 and 220.4 L m−2 h-1) and selectivity (dye rejection up to 83.7% and 65.4%) in both dead-end and cross-flow ultrafiltration systems, respectively, thanks to the improved hydrophilicity, porosity and surface negative charge of the membranes.

Проблеми ідентифікації пародонтопатогенної мікрофлори та можливість їх вирішення за допомогою математичного моделювання ефективного діелектричного відгуку на прикладі S. aureus (референсного штаму)

The aim of the study was to create a multiparametric mathematical model describing the dielectric response of Staphylococcus aureus suspensions for its further use to interpret dielectric spectra and identify the parameters of periodontopathogenic bacterial flora, that would optimize diagnostic processes in periodontology. Materials and methods. The study was based on the use of the Bergman-Milton analytical spectral representation for the effective dielectric response, generalized to the case of inclusions (bacteria), which can be modeled as core-shell- spheroids. The developed software using the Matlab numerical computing environment allowed us to determine the effective dielectric conductivity and dielectric losses as a function of material and geometrical parameters of the bacterial suspension. The material parameters and the inclusion sizes (Staphylococcus aures – S. Aureus bacteria) were taken from literature sources; geometrical parameters characterizing the bacteria shape were considered as model variables. To illustrate the application of the method, the spectral density function of the simplest kind, sush as a homogeneous distribution of spheroidal shapes with a median corresponding to the spherical shape, was used. Results. The spectra of the real and imaginary part of the effective dielectric function εeff of S. Aureus aqueous suspensions in the frequency range of 1–104 KHz at different values of a nonsphericity parameter, characterizing the fluctuations of the bacterial shape around the spherical one, were calculated. It is shown that shape fluctuations can significantly affect Re εeff only at low frequencies, where they increase Re εeff. At high frequencies, the shape fluctuations only slightly reduce Re εeff. The imaginary part of the effective dielectric function (dielectric losses) shows a weak dependence on the nonsphericity parameter with in the entire frequency range. Other calculations indicate that the increase in the electrical conductivity of the plasma membrane, which characterizes S. Aureus dead bacteria, is accompanied by a marked decrease in Re εeff at frequencies below 1 MHz. Conclusion. By the example of S. Aureus, we demonstrated the use of a generalized spectral model of the effective dielectric response for the interpretation of the dielectric spectra of pathogenic microorganisms. The simplicity and analyticity of the model make it a convenient and promising tool for biophysical and medical studies. The generalized spectral model can be used to solve thel direct problem, namely to determine the influence of material and geometrical parameters of bacteria on their dielectric spectra, as well as to solve the inverse problem, which consists in finding the model parameters by processing the experimental dielectric spectra.

Formulasi Dan Uji Aktivitas Anti Bakteri Masker Gel Peel-Off Ekstrak Daun Pacar Air (Impatiens balsamina linn.) Dengan Kombinasi Basis PVA dan HPMC

Peel-off gel mask is one of the practical masks, because after dry the mask can be directly removed without rinsing. In this research, a peel-off gel mask formulation from a pacar air leaf extract (Impatiens balsamina Linn.) Has antibacterial activity against Staphylococus Aureus bacteria. The peel-off gel mask is prepared in 3 formulas by varying the PVA and HPMC concentrations of F1 (1: 2,6), F2 (1: 3), F3 (1: 3,5). Evaluation of gel quality used is organoleptic test, viscosity, adhesion, drying time, dispersion, pH. Testing of antibacterial activity was done by using the diffusion method of wells. The positive control used was clindamycin phosphate 1.2%, the negative control used was the base of the peel-off gel mask. Results of physical evaluation and antibacterial activity test were analyzed using SPSS software with trust level of 0.05. Based on the results obtained F3 is the optimum formula, because F3 has a corresponding pH of 5.2 ± 0.10, the corresponding depth of 6.85 ± 0.13, the fastest drying time of 21.68 ± 0.33 and the anti-activity the best bacteria. Based on testing of antibacterial activity of F1, F2 and F3 have antibacterial activity against Staphylococus Aureus bacteria.

Biosynthesis of Ag nanoparticles using Cynara cardunculus leaf extract: Evaluation of their antibacterial and electrochemical activity

Abstract The present study reports the green synthesis of silver nanoparticles (AgNP’s) using Cynara cardunculus leaf extract in aqueous media. The silver ions reduction was carried out by the antioxidant component presents in the Cynara cardunculus. Transmission electron micrographs revealed that the synthesized AgNP’s exhibits semi-spherical morphology and the particle size was less than 45 nm. The energy dispersive X-ray analysis (EDS) spectrum revealed the presence of silver. Complementary, The AgNP’s solution exhibited an absorption peak at 435 nm, corresponding to surface plasmon resonance of AgNP’s. Fourier transform infrared (FT-IR) spectra showed the presence of the organic components responsible for reduction and stabilizing the nanoparticles. X-ray photoelectron spectroscopy (XPS) was employed to elucidate the chemical state and near surface composition of the AgNP’s obtained from Cynara cardunculus leaf extract. The antibacterial effect of the AgNP’s synthesized was tested by the disk diffusion method against E. Coli and S. Aureus bacteria. The electrochemical response of the nanoparticles was evaluated by cyclic voltammogram. The antibacterial and electrochemical activity of the AgNP’s were described in this work.

Synthesis and Property of Ag(NP)/catechin/Gelatin Nanofiber

Nanomaterial play important role future industry such as for the medical, food, pharmaceutical and cosmetic industry. Ag (NP) and catechin exhibit antibacterial property. Ag(NP) with diameter around 15 nm was synthesis by microwaved method. We have successfully produce Ag(NP)/catechin/gelatin nanofiber composite by electrospinning process. Ag(NP)/catechin/gelatin nanofiber was synthesized by using gelatin from tuna fish, polyethylene oxide (PEO), acetic acid as solvent and silver nanoparticle(NP)/catechin as bioactive component, respectively. Morphology and structure of bioactive catechin-gelatin nanofiber were characterized by scanning electron microscopy (SEM) and fourier transform infrared spectroscopy (FTIR), respectively. SEM analysis showed that morphology of nanofiber composite was smooth and had average diameter 398.97 nm. FTIR analysis results were used to confirm structure of catechin-gelatin nanofiber. It was confirmed by FTIR that specific vibration band peak amide A (N-H) at 3286,209 cm-1, amide B (N-H) 3069,396 cm-1, amide I (C=O) at 1643,813 cm-1, amide II (N-H and CN) at 1538,949 cm-1, amide III (C-N) at 1276,789 cm-1, C-O-C from polyethylene oxide at 1146,418 cm-1, respectively. When examined to S. Aureus bacteria, Ag/catechin/gelatin nanofiber show inhabitation performance around 40.44%. Ag(NP)/catechin/gelatin nanofiber has potential application antibacterial medical application.