Aucheri Boiss Research Articles

Bioassay Guided Isolation and α-Glucosidase Inhibition Studies of a New Sesquiterpene from Ochradenus aucheri.

The aim of the current study was to explore the anti-diabetic potential of Ochradenus aucheri Boiss (O. aucheri). All the fractions of O. aucheri were evaluated for α-glucosidase inhibition, followed by bioassay-guided isolation which resulted in a new sesquiterpenoid, as a potential α-glucosidase inhibitor. The preliminary screening showed that all the fractions including n-hexane (38.0 ± 1.38 μg/mL), dichloromethane (92.6 ± 6.18 μg/mL), ethyl acetate (29.2 ± 0.51 μg/mL) and n-butanol (361.8 ± 5.80 μg/mL) displayed significant α-glucosidase inhibitory activity. The activity-directed fractionation and purification of ethyl acetate fraction led to the isolation of one new sesquiterpenoid, Jardenol (1), and two known metabolites: β-stitosterol-3-O-β-D-glucopyranoside (2) and β-Sitosterol (3). To the best of our knowledge, these metabolites have not been isolated from this plant previously. The structure of the new metabolite 1 was confirmed through 1D and 2D NMR spectroscopy, and MS analysis. Compound 1 showed significant α-glucosidase inhibition with an IC50 value of 138.2 ± 2.43 μg/mL as compared to positive control acarbose (IC50 = 942.0 ± 0.60 μg/mL). Additionally, in-silico docking was employed to predict the binding mechanism of compound 1 in the active site of the target enzyme, α-glucosidase. The docking results suggested that the compound forms strong interactions at the catalytic site of α-glucosidase. The results of the present study indicated that the newly purified secondary metabolite, Jardenol, can be a promising anti-diabetic compound.

Extraction and Identification of saponin extracts from Lepidium aucheri Boiss and antifungal properties evaluation

Extraction and Identification of saponin extracts from Lepidium aucheri Boiss and antifungal properties evaluation

Volatile components of Ferulago aucheri Boiss. (Apiaceae)

Ferulago aucheri Boiss, which belongs to the Apiaceae family, is distributed from Türkiye to the Caucasus. Its chemical composition may vary depending on the region where it grows, climate conditions and topography. Essential oil components were determined using two different techniques (hydrodistilled and headspace-solid phase microextraction HS-SPME) with the species collected from Nallıhan district of Ankara, Türkiye. In the present study, essential oil and headspace volatiles of aerial parts of Ferulago aucheri Boiss. were analyzed by GC-GC/MS. Thirty-five components were characterized, representing 99.6% of the oil by hydrodistilled and thirty components were characterized, representing 98.7% by HS-SPME. In both techniques, the main substances were identified as α-pinene, limonene, and δ-3-carene. In previous studies, it has been observed that the main components are in different amounts, and some studies even have different main components.

The Effect of Hydroalcoholic Extract of Quercus brantii and Artemisia aucheri Boiss Against Trichomonas vaginalis In vitro.

Trichomoniasis is the most common sexually transmitted protozoan infection worldwide. Metronidazole is widely considered as the drug of choice for treating of trichomoniasis but considering its potential side effects, we aimed to assess the therapeutic influences of hydro-alcoholic extracts of Quercus brantii and Artemisia aucheri Boiss as alternative medications against Trichomonas vaginalis (T. vaginalis). The trophozoites were cultured in TYI-S-33 medium at a density of 5x105 trophozoites/mL. Subsequently, they were incubated with varying concentrations of the plant extracts (32, 64, 125, 250, 500, and 1,000 μg/mL) and metronidazole (16, 32, 64, 125, 250, and 500 μg/mL), as the positive control. The number of trophozoites in each well plate was quantified after 2, 4, 6, 24, 48, and 72 hours using trypan blue staining. Finally, the viability of the parasite was assessed by vital methylene blue staining. The hydro-alcoholic extracts of Q. brantii and A. aucheri Boiss at concentrations of 125, 250, 500, and 1,000 μg/mL demonstrated significant efficacy against the parasite. Our findings indicated that the minimum effective concentrations were 125 μg/mL and hydro-alcoholic extracts of Q. brantii and A. aucheri Boiss have the ability to effectively eliminate T. vaginalis after 48 and 72 hours of treatment. The findings of the present study showed that hydro-alcoholic extract of Q. brantii and A. aucheri Boiss can induce death in T. vaginalis. However, further complementary in vivo studies are needed to assess the components of these plants in the treatment of T. vaginalis.

Investigation on phytochemical constituents of Achillea aucheri Boiss. Endemic to Iranian flora accompanied by antioxidant and antimicrobial evaluations

In traditional medicine, the genus Achillea is utilized for its anti-inflammatory, antispasmodic, and diuretic properties. Achillea aucheri Boiss., species native to Iranian flora was studied due to the Achillea genus's rich supply of phytochemical substances and the absence of essential phytochemical investigations.The methanolic extract (ME) from aerial parts of A. aucheri was fractionated using a C-18 pre-packed cartridge and vacuum liquid chromatography, followed by chromatographic separation using a reversed-phase preparative HPLC. The isolated components were structurally elucidated using 1D and 2D-NMR spectroscopy. Furthermore, essential oil from A. aucheri was isolated through hydrodistillation. Analysis of the essential oil was done through gas chromatography–mass spectrometry (GC-MS) and GC-FID. Free-radical scavenging activity, total flavonoid and phenolic content of the ME were determined using the DPPH assay, AlCl3, and Folin-Ciocalteu reagents, respectively. Anti-microbial activity of the A. aucheri essential oil was investigated against different bacteria and fungus via disk diffusion and micro-dilution methods.Structure elucidation of the purified compounds revealed presence of one major flavonoid (1) and a sesquiterpene (2) in ME. From the A. aucheri essential oil, α-thujone (45.6%), artemisia alcohol (26.5%), and yomogi alcohol (8.8%) were the major constituents. The total flavonoid, phenolic content of the ME were 39.70 ± 0.05 mgQE/gE and 66.70 ± 0.08 mgGAE/gE, respectively, while IC50 value in DPPH assay was 86.28 ± 0.01 μg/mL. Concerning the achieved antimicrobial activity, maximum diameter of inhibition zone of the essential oil was obtained against S. aureus (31.5 ± 0.5 mm) followed by S. epidermidis (13.5 ± 0.7 mm) and M. luteus (11.5 ± 0.5 mm). Additionally, the minimum inhibitory concentration (MIC) values for S. aureus, S. epidermidis, and M. luteus were recorded as 2.5, 2.5 and 1.25% (v/v), respectively. Exhibited activity could be explained to the presence of α-thujone, artemisia alcohol and yomogi alcohol, the most abundant phytochemicals of A. aucheri essential oil.

Variation of phytochemical constituents, antioxidant, antibacterial, antifungal, and anti-inflammatory properties of Grantia aucheri (Boiss.) at different growth stages

Phyto-natural products are deeply associated with ethno-medicinal practices with less or more scientific validation for curing many vital diseases. Thus, the present study was carried out to asses chemical constituents and bioactivities of Grantia aucheri Boiss at different growth stages (vegetative, flowering, and seeding). For this aim, the chemical composition of G. aucheri ethanolic extracts was analyzed by gas chromatography with mass spectrometry detection (GC–MS), their total phenolics, total flavonoids, total tannins, and total anthocyanins concentrations were also spectrophotometrically determined. Antifungal and antibacterial activities were determined against three pathogenic fungi and six human pathogenic bacteria. Furthermore, antioxidant activity (DPPH and ABTS scavenging) and anti-inflammatory activity (Human Red Blood Cell Stabilization Method) were evaluated. The highest content of total phenolics, total flavonoids, total tannins, and total anthocyanins were established in the extract of G. aucheri at its flowering stage. Such phyto-compounds as boranyl acetate, β-himachalene and himachalol were major compounds found among 34 chemical constituents identified. The best antioxidant, anti-inflammatory, antifungal, and antibacterial activities were also found for this extract. Its phytochemicals presented bactericidal activities, mainly against Staphylococcus aureus, Bacillus subtilis, and Streptococcus pyogenes along with moderate fungicidal activity, however, it was less effective than the first one. Apart from antioxidant, antimicrobial, and anti-inflammatory activities, chemical constituents of G. aucheri may be potential alternative biomedical applications to reduce synthetic chemicals drugs.

Blood Hemolytic Activity and Acute Toxicity of Saponins extract from Lepudium aucheri Boiss.

The hemolytic activity process was applied to erythrocytes by an analyst, as well as the lysis of erythrocytes resulted in a positive test result. The L. aucheri Boiss. extracts are high in triterpenoids saponins, according to the findings. Both extracts were used in acute toxicity in vivo research on four groups of rats (six rats in each group). After 72 hours of treatment with various concentrations of terpenoids saponins extracts (25, 50, and 100 mg/kg BW), no mortality was detected in the rats of the trials. This demonstrated that both extracts are harmless when taken orally.

Scientific Studies on Ochradenus Aucheri Boiss

Ochradenus aucheri Boiss. (Jesh) belonging to family Resedaceae usually found in sandy areas, hillsides, and mountains. Common in southern and eastern Iran to Pakistan and Muscat. Very common throughout mountains at lower elevation and on alluvial gravels in the northern Emirates. Rapidly colonizes new roadsides, e.g. around Hatta (Western, 1989). Unripe fruits eaten as treatment for digestive problems

RAPD-based evaluation of genetic diversity among populations of the Iranian endemic species Rhabdosciadium aucheri Boiss. (Apiaceae).

Rhabosciadium aucheri is an Iranian endemic herbaceous species that grows in the west, center, and south regions of Iran. In the present study, genetic variation of 70 individuals belonging to seven natural populations of four provinces was investigated using Random Amplified Polymorphic DNA (RAPD) markers. Ten out of twenty-two RAPD primers employed in this study, generated 110 highly amplified and reproducible loci and a mean of 11.1 bands per primer and 48.13% of polymorphism was obtained. According to our results, the primer OPA10 presented the highest effective number of alleles, Shannon's index, and genetic diversity. The highest value of genetic identity (0.916) was determined between Hamadan, Nahavand and Hamadan, Alvand Mts. populations and the highest genetic distance (0.277) was observed between Hamadan, Asadabad and Kurdistan, Qorveh populations. Therefore, there is an obvious correlation between genetic diversity and geographical distribution. PCA was obtained based on RAPD molecular data and Neighbor Joining (NJ) dendrogram was provided successively. Similar results were attained employing UPGMA and Neighbor Joining dendrograms, supported by PCA ordination plot. Overall, almost moderate level of polymorphism was obtained by RAPD molecular markers at the population level that shows a reasonable amount of intraspecific variability. The reason might be due to the low level of gene flow between populations that could give rise to high genetic differentiation.

استخلاص الزيوت الاساسية من نبات الرشاد البري(Lepidium aucheri boiss) و دراسة فعاليتها الحيوية

The present study amide to extracts the essential oils from Lepidium aucheri boiss ,where it was collected in March 2015 from the northern of Nasiriyah city and it was classified . it was extracted from plants by Clevenger's Apparatus to be finally getting around 26.45g of essential oil of 24Kg plant .Then study the bioactivity of this essential oils ,the extracted essential oils from the plant didn’t showed any toxicity toward the red blood cells. The antibacterial properties was used five types of bacteria, Cram positive strain (Staphylococcus aureus, Streptococcus pyogenes and Enterobacter sp.) and Cram negative strain (Escherichia coli, Pseudomonas aeruginosa),These moleules showed antimicrobial activites against gram negative and gram positive bacterial . The activity of the concentration 100% showed the highest percentage inhibition there are (20mm) towards bacteria Staphylococcus and E.coli bacteria showed less inhibition ratio there are (7mm) at the same concentration .In the test of antifungal properties used four types of fungus which Asperggillus niger and Asperggillus terrus and Asperggillus flavus and Candida albicans .The results showed the highest inhibition of the fungus at concentration 100% towards A.niger where the scale inhibition is 12 mm, while the other fungi have shown high resistance toward all the prepared concentration ,especially C.albicans. تشير الدراسة الحالية الى استخلاص الزيوت الاساسية من نبات الرشاد البري (lepidium aucheri boiss) ، اذ تم جمع النبات في اذار 2015 من شمال مدينة الناصرية ثم تم تصنيفه و استخلصت الزيوت الاساسية من النبات بواسطة جهاز كلافينجر (Clevenger's Apparatus) ، ليتم الحصـول في النهاية على ما يقارب 26.45 g من الزيوت الاساسية من 24 Kg من النبات ، ثم تم دراسة الفعالية الحيوية لها . لم تظهر الزيوت الاساسية المستخلصة من النبات اي سمية تجاه كريات الدم الحمراء ، اذ تم دراسة الخصائص المضادة للبكتيريا باستخدام خمسة انواع من البكتيريا ، و هي البكتيريا الموجبة لصبغة كرام Staphylococcus aureus , Streptococcus pyogenes and Enterobacter sp. ) ، و البكتيريا السالبة لصبغة كرام (Escherichia coli and Pseudomonas aeruginosa) ، للتحقق من فعالية الزيوت الاساسية المستخلصة ، حيث اظهرت النتائج بان لها فعالية مضادة للبكتيريا متفاوتة مقارنة بالمضاد البكتيري السبرودار(Ciprodar) . حيث عند التركيز 100% اظهرت البكتريا Staphylococcus aureus اعلى نسبة تثبيط ، حيث كان قطر منطقة التثبيط هو (20 mm) واظهرت البكتيريا E.coli اقل نسـبة تثبيط حيث كان قطر منطقة التثبيط هو (7 mm) عند نفس التركيز . اما الخصائص المضادة للفطريات تم دراستها على اربع انواع من الفطريات وهي (Asperggillus niger و Asperggillus terrus و Asperggillus flavus و Candida albicans) . النتائج اظهرت اعلى فعالية عند التركيز 100 % للفطر A.niger ، حيث كان قطر منطقة التثبيط هو (mm (12 ، اما الفطريات الاخرى فقد ابدت مقاومة عالية خاصة C.albicans التي لم تتأثر بجميع التراكيز المحضرة.

Effect of some plant species on fatty acid composition and mineral contents of Ferulago, Prangos, Ferula, and Marrubium seed and oils

The oil contents of seeds were between 4.92% (Ferulago aucheri) and 16.47% (Ferula parva). The highest oleic acid contents were detected in Ferulago syraea (67.15%), Ferulago pauciradiata (67.15%), Ferulago pachyloba (68.82%), Ferulago syriaca (66.16%), Prangos uechtritzii (60.37%), Ferula tingitana (77.26%), and Ferula szowitsiana (58.81%) seed oils. Oleic acid contents of Ferulago spp. (except F. aucheri) oils were found higher than those of other plant seed oils used in experiment (except F. tingitana). The behenic acid content (75.38%) of F. aucheri were significantly higher than other samples. The K contents of plants ranged between 2,888.41 mg/kg (F. aucheri Boiss.) and 21,601.26 mg/kg (Ferula lycia Boiss.); Ca contents of plants ranged between 5,041.86 (Marrubium catariifolium Desr.) and 39,858.36 mg/kg (F. tingitana L.). In addition, the P contents of plants remained between 299.25 (M. catariifolium Desr.) and 4,674.64 mg/kg (Marrubium trachyticum Boiss.). The protein contents of plant seeds ranged between 1.75 (F. aucheri Boiss.) and 5.40% (M. trachyticum Boiss.). Practical applications Plant seed oils have been used for centuries by rural communities in foods, medicines, cosmetics, and fuel. They have been observed to carry excellent nutritional, nutraceutical, and pharmaceutical values. They are provided with good quantities of different nutrients and important fatty acids. The study will help to understand the differences in fatty acid and mineral composition of different plants from Apiaceae and Lamiaceae families.

Study of two-stage ohmic hydro-extraction of essential oil from Artemisia aucheri Boiss.: Antioxidant and antimicrobial characteristics

The effect of two-stage ohmic-assisted hydrodistillation (TSOH) on the extraction and characteristics of essential oils (EOs) from the Artemisia aucheri Boiss. was studied, and the results were compared to conventional hydrodistillation (HD). According to the results, the yield of EOs obtained through TSOH was almost 30% higher than those extracted by HD in nearly one-quarter of a time used by the HD. Scanning electron micrographs of A. aucheri leaves showed almost complete eruption of EO glands and their surrounding area in TSOH extraction method, hence achieving higher yield. The components of the EOs obtained through TSOH were only slightly different from those of HD. GC/MS analysis indicated some differences in the quantity of the main components, too. The main components of EOs were identified as Thymol, Linalool, Geraniol, Camphor, and 1, 8-Cineole, Davana ether and Cis-Davanone. Thymol (~17%) and Cis-Davanone (~23%) were the highest quantity in the EOs extracted from TSOH and HD, respectively. The variation of antioxidant and antimicrobial activities of the EOs may be attributed to these differences in the percentage of the main components. The radical scavenging activity of the EOs obtained by TSOH was almost twice that of HD. Based on antimicrobial activity assays, the EOs were efficient against S. aureus (a Gram-positive), E. coli (a Gram-negative), and S. cerevisiae (yeast). However, the efficacy was higher in gram-positive than gram-negative bacteria and yeast. The results indicate TSOH has a potential to produce EOs from herbal plants at a faster rate, higher yield, being probably more efficient in terms of energy although having similar antimicrobial and antioxidant efficacy.

A New Species of Hendrella (Diptera, Tephritidae) from South-Eastern Iran

AbstractThe genus Hendrella Munro, 1938 hitherto was known to include 8 species occurring in the Palaearctic Region. All species of known biology are associated with Artemisia spp. (Asteraceae). An additional species with similar biology, H. kermanensis sp. n., recently discovered in Iran is described. It is very similar to H. caloptera in the coloration of antenna and femora, as well as presence of one hyaline spot in cell r1, but differs from it by the shape of the first flagellomere and also by the wing pattern details. Larvae of H. kermanensis sp. n. induce stem galls in Artemisia aucheri Boiss. of the tribe Anthemideae (Asteraceae).

Extracting essential oils from wild cress (Lepidium aucheri boiss ) and studying their biological effectiveness

The present study amide to extracts the essential oils from Lepidium aucheri boiss ,where it was collectedin March 2015 from the northern of Nasiriyah city and it was classified . it was extracted from plantsby Clevenger's Apparatus to be finally getting around 26.45g of essential oil of 24Kg plant .Then study thebioactivity of this essential oils ,the extracted essential oils from the plant didn’t showed any toxicity toward the red blood cells. The antibacterial properties was used five types of bacteria, Cram positive strain (Staphylococcus aureus, Streptococcus pyogenes and Enterobacter sp.) and Cram negative strain (Escherichia coli, Pseudomonas aeruginosa),These moleules showed antimicrobial activites against gram negative and gram positive bacterial .The activity of the concentration 100% showed the highest percentage inhibition there are (20mm) towards bacteria Staphylococcus and E.coli bacteria showed less inhibition ratio there are (7mm) at the same concentration .In the test of antifungal properties used four types of fungus which Asperggillus niger and Asperggillus terrus and Asperggillus flavus and Candida albicans .The results showed the highest inhibition of the fungus at concentration 100% towards A.niger where the scale inhibition is 12 mm, while the other fungi have shown high resistance toward all the prepared concentration ,especially C.albicans.

Physiological targets of salicylic acid on Artemisia aucheri BOISS as a medicinal and aromatic plant grown under in vitro drought stress.

Background Artemisia aucheri BOISS is a medicinal and aromatic plant, which is endemic to mountainous areas of Iran and surroundings. In this study, we investigated the alleviating effects of salicylic acid (SA) pretreatment (0.01 and 0.1 mM) on A. aucheri under in vitro drought stress induced by 2 and 4% polyethylene glycol (PEG/6000).ResultsPlants exposed to PEG stress showed higher levels of H2O2, MDA and electrolyte leakage compared with control. While SA pretreatment decreased these parameters under PEG stress significantly. The activity of CAT, POD, APX, SOD and GR positively changed with PEG and more induction in activity of antioxidant enzymes was observed in SA-pretreated plants under PEG stress. Furthermore, ASA, GSH and their redox ratios (ASC/DHA and GSH/GSSG) enhanced with SA pretreatments. Analysis of our data revealed that MDA, DHA and H2O2 were the best targets for SA under in vitro PEG treatment for A. aucheri plants.ConclusionsSalicylic acid as a signal molecule mitigated adverse effects of PEG-simulated drought stress on A. aucheri under in vitro condition by improving the activity of antioxidant enzymes. In addition, protective role of SA was also related to promotion of ascorbate–glutathione cycle.

Rhus aucheri Boiss, an Omani herbal medicine: Identification and in-vitro antioxidant and antibacterial potentials of its leaves' extracts

Rhus aucheri Boiss is a popular Omani herbal medicinal plant used as an antiseptic, astringent and homeostatic. In this study we evaluated, the antioxidant and antibacterial potential of R. aucheri Boiss leaves using an in-vitro model. Three green solvents, i.e. water ethanol and aqueous glycerol, were employed to access the extraction of polyphenols and flavonoids. We found the highest polyphenols and flavonoids extraction for aqueous glycerol extracts The antioxidant capacity was assessed using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical reducing abilities of the three liquid crude extracts (LCEs) and was recorded to be high in water and aqueous glycerol LCEs (91 and 83.1%) respectively. The screening of antibacterial potential was gauged against gram positive bacterial strains namely Staphylococcus aureus (ATCC® 29213™), Staphylococcus aureus (ATCC® 25923™) and Enterococcus faecalis (ATCC® 29212™) as well as one gram negative strain of Escherichia coli. The three LCEs were found to be fairly active against the microbial strains tested. Further, we recorded the scanning electron microscopic (SEM) images of the leaves of R. aucheri Boiss leaves to underpin the botanical characters of the leaves so that any future studies of this plant will have a sound basis.

The impact of salicylic acid on some physiological responses of Artemisia aucheri Boiss. under in vitro drought stress

Salicylic acid (SA) is an important plant regulator which is involved in growth, development, and response to stress. This study was aimed to evaluate some physiological and biochemical responses of Artemisia aucheri Boiss. under drought stress after exogenous SA treatment. Experiment was performed in vitro. Polyethylene glycol (PEG/6000) with 0, 2 and 4 % (w/v) was used in MS medium to simulate drought stress and different concentrations of SA (0, 0.01 and 0.1mM) were added. After four weeks, SA alleviated the negative effects of PEG on dry and fresh mass as well as chlorophyll and carotenoid contents. Under drought stress, application of SA decreased storage polysaccharides and increased soluble carbohydrates respectively. Although PEG had no significant effect on flavonoid content, it increased significantly anthocyanin and total phenol content, total antioxidant capacity, PAL (phenylalanine ammonia-lyase) and TAL (tyrosine ammonia-lyase) activity and SA treatment improved these parameters significantly. According to the current data, it was concluded that SA increased drought tolerance of Artemisia aucheri by increasing biosynthesis of phenolic compounds, improvement of TAL and PAL activity as well as also by increased content of soluble carbohydrates.

Effect of Phenological Stages on Chemical Composition of Platychaete aucheri Boiss. as an Important Endemic Shrub in Iran

Platychaete aucheri Boiss. is a native long-lived, perennial shrub adapted to the arid regions of southern Iran. The objective of this study was to evaluate the nutritive composition of the endemic forage species, P. aucheri Boiss., in arid communal grazing area of southern Iran. Aerial parts of plants were harvested at three vegetation stages (prebud, flowering, and maturity) in the natural habitats of Jahrom area. The chemical analysis included were crude protein (CP), ash, metabolizable energy (ME), dry matter digestibility (DMD), neutral detergent fiber (NDF), and acid detergent fiber (ADF). Growth season had a significant effect on the forage quality of P. aucheri Boiss. The foliage CP content and ME value ranged from 3.56 to 4.56 % and from 6.11 to 8.07 MJ kg−1, respectively. The DMD and CP declined when plant matured. Phenological stage indicated a significant effect on NDF and ADF values, increasing from flowering to maturity stage. The highest crude protein content was 4.56 %, and it would be concluded that this plant alone could not meet nutritional requirements of grazing animals. In general, the best grazing time is in the flowering stage when the forage has higher nutritive composition.

Identification and Comparison of Essential Oil Composition of Artemisia sieberi and Artemisia aucheri Cultivated in the South of Iran

In this study qualitative and quantitative chemical composition of two sagebrush species (Artemisia sieberi Besser and Artemisia aucheri Boiss.) were evaluated and compared. For this purpose, sagebrush seeds were grown in Medicinal Plants Research Site at Estahban Branch, Islamic Azad University, Estahban, Iran during 2012 growing season. At the beginning of the flowering stage, shoot parts of the plants were harvested and dried in shade. The essential oil was extracted by hydro-distillation method using Clevenger apparatus. The chemical composition of the essential oil was investigated by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). In total, 44 different constituents were identified in Artemisia aucheri, constituting 99.99% of the essential oil. The main constituents identified in Artemisia aucheri were myrcene (19.83%), linalool (17.98%), lavandulol (7.30%), bornyl acetate (6.72%), E-nerolidol (6.28%), davanone (5.46%), 1,8-cineole (4.51%), borneol (4.38%) and neryl propanoate (3.55%). In case of Artemisia sieberi, 38 different constituents were identified, comprising 100% of the essential oil, including camphor (29.50%), cis-thujone (22.58%), 1,8-cineole (12.91%), trans-thujone (10.60%) and camphene (5.05%). The results indicated that camphene (5.05%), cis-thujone (22.58%), trans-thujone (10.60%) and camphor (29.50%) were identified just in Artemisia sieberi. By contrast, myrcene (19.83%), linalool (17.98%), borneol (4.38%), lavandulol (7.30%), bornyl acetate (6.72%), neryl propanoate (3.55%), E-nerolidol (6.28%) and davanone (5.46%) were identified just in Artemisia aucheri.

An Investigation on Essential Oils of Platychaete aucheri Boiss. as an Endemic Plant in Desert Ecosystems

Due to the necessity of prevention of desertification, in range renovation programs, the identification of drought resistant plants and also using multiple purposes of such a plant species is extremely important. The expanse and the protection of medicinal plants can be an income source for local people in desert region and also provide part of pharmacy factory requirements. According to phytogeography classification, Platychaete aucheri belongs to the Khalij-Omani region. This endemic shrub of Iran grows at Fars province (near Jahrom and near Firoozabad), Kerman and Hormozgan. In the present research Platychaete aucheri has been considered in regard to phytochemical characteristic. The aerial parts of Pl. aucheri were collected in the flowering stage. The collected dried samples were extracted by hydrodistillation method. The amount and percentage of essential oil components was 1.76%. 48 components representing 91.3% of the total oil were characterized. The results of chemical components analysis of essential oils has been shown that the main identified components are myrtenol (34.66%), borneol (18.17%), trans-verbenol (5.7%), bornyl acetate (4.31%), cis-verbenol (3.29%) and 1,8-cineole (2.42%), respectively. It seems that borneol, transverbenol and 1,8-cineole play as an anti-quality factor in animal feed. This issue confirms non-palatability of Pl. aucheri in the flowering stage. Hence, one of the rangeland management principles is prevention of entrance non-native and exotic livestock to the rangelands with toxic plants.