Effective use of nonthermal atmospheric plasma (NTAP) to strengthen adhesive-dentin interfacial bonding while disinfecting with chlorhexidine (CHX). NTAP application at different time intervals on the dentinal shear bond strength (SBS) after pretreatment with 2% CHX as a cavity disinfectant. The design of the study was an in vitro study. Forty permanent mandibular teeth were collected (n = 40) and the occlusal surfaces were flattened. For, all the specimens 37% phosphoric acid etching was done followed by pretreatment with 2% CHX as cavity disinfectant for 5 s. According to the surface treatment, divided into four groups of n = 10. Group I (Control): No NTAP pretreatment was done. Group II: NTAP pretreatment done for 15 s. Group III: NTAP pretreatment done for 30 s. Group IV: NTAP pretreatment done for 45 s. Later, all the specimens were treated with a bonding agent, incremental build-up of composite resin on the dentin surface was done and evaluation of SBS was done. Analyzed using One-way analysis of variance with a post hoc Tukey's test (P < 0.05). Two percent CHX pretreatment as cavity disinfectant followed by NTAP application for 30 s (Group III) exhibited greater values compared to the control group (Group I). Two percent CHX pretreatment as cavity disinfectant followed by NTAP pretreatment for 30 s was found to exhibit better bond strength values compared to 15 s as well as 45 s.