Atmospheric Ionization Research Articles

Mapping Reaction Pathways by In Situ Step Sweep Voltammetry Flow Electrochemical Mass Spectrometry.

A step sweep voltammetry (SSV) flow electrochemical (EC) mass spectrometry (MS) platform was developed for real-time and in situ mapping of EC reaction pathways. By integrating a flow EC cell into the pneumatic spray nozzle followed by atmospheric chemical ionization, this setup was capable of in situ MS monitoring of short-lived EC intermediates with enhanced sensitivity. This setup also realized precise measurement and control of the electrode potential during in situ EC-MS analysis, which can provide detailed information on the interplay of reaction pathways under different electrode potentials. Taking the EC reductive cross coupling of nitroarenes with arylboronic acids as an example, SSV-MS had identified 13 compounds among four reaction pathways. Among these, the electrode potential of active nitrene and cross coupling intermediates were measured for the first time and the structure of the nitroso coupling complex was also confirmed by MS. With the systematic measurement of electrode potential of the intermediates and products, SSV-MS had clearly mapped out the synergies and competitions between different reaction pathways, offering key insights for optimizing reaction conditions and investigating reaction mechanisms for EC research.

Acute exposure to LPS induces cardiac dysfunction via the activation of the NLRP3 inflammasome

Systemic inflammation contributes to left ventricular (LV) dysfunction, however the role of the NLRP3 inflammasome in LV dysfunction in acute inflammatory conditions is unclear. This study investigated the role of the NLRP3 inflammasome in acute (24 h) cardiac structural and functional changes in vivo and in vitro in lipopolysaccharide (LPS)-induced inflammation. LPS-treated Sprague-Dawley (SD) rats showed increased LPS metabolite abundance in their LVs as measured by atmospheric pressure matrix-assisted laser desorption ionisation (AP-MALDI) mass spectrometry imaging (MSI). Echocardiography and histology showed that in LPS-exposed rats, LV internal diameter was decreased, with evidence of macrophage infiltration and oedema. However, there were no changes in LV wall thickness or collagen volume. Additionally, LPS-exposed rats exhibited impaired LV relaxation, potentially contributing to decreased stroke volume. While global systolic function was preserved, LPS exposure in SD rats resulted in impaired myocardial deformation assessed by speckle-tracking echocardiography. Exposure to LPS resulted in upregulation of the expression of components of the NLRP3 inflammasome in rodents. In vitro LPS exposure resulted in increased gene expression of NLRP3 and downstream cytokines IL-1β and IL-18, antioxidant SOD2, and elevated markers of pyroptosis (GSDMD) which were inhibited by treatment with a NLRP3 antagonist. However, LPS-induced increases in the gene expression of apoptosic markers (BAX/Bcl2) were not impacted by NLRP3 antagonism. These findings suggest that inflammation induced adverse cardiac structural and functional changes is, at least in part, mediated by the NLRP3 inflammasome in acute, high-grade inflammatory states. In addition, in vitro findings suggest that while the NLRP3 inflammasome mediates pyroptotic pathways, regulation of apoptosis that is independent of the inflammasome.

Multiphysical description of atmospheric pressure interface chemical ionisation in MION2 and Eisele type inlets

Abstract. Chemical ionisation inlets are fundamental instrument components in chemical ionisation mass spectrometry (CIMS). However, the sample gas and reagent ion trajectories are often understood only in a general and qualitative manner. Here, we evaluate two atmospheric pressure interface chemical ionisation inlets (MION2 and Eisele type inlet) with 3D computational fluid dynamics physicochemical models regarding the reagent ion and sample gas trajectories and estimate their efficiencies of reagent ion production, reagent ion delivery from the ion source volume into the ion–molecule mixing region, and the interaction between reagent ions and target molecules. The models are validated by laboratory measurements and quantitatively reproduce observed sensitivities to tuning parameters, including ion currents and changes in mass spectra. The study elucidates how the different transport and chemical reactions proceed within the studied inlets, where space charge can already be relevant at ion concentrations as low as 107 cm−3, and compares the two investigated inlet models. The models provide insights into how to operate the inlets and will help in the development of future inlets that further enhance the capability of CIMS.

Venusian ion escape under extreme conditions: A dynamic pressure and temperature simulation study

We investigated the response of the Venusian atmospheric ion escape under the effect of interplanetary coronal mass ejections (ICMEs) using the Latmos Hybrid Simulation (LatHyS). In particular, we focused on the influence of extreme ICME dynamic pressures and temperatures, with the temperature being a parameter that has not been extensively studied in the past. Simulations were performed for two different dynamic pressures and three different temperatures. For the case of the dynamic pressure simulations, a density and a velocity enhancement event were studied separately. The H$^ $ and O$^ $ ion escape was then examined and compared for different escape channels. In both dynamic pressure enhancement cases, we find that there is no clear dependence of the O$^ $ ion escape on the dynamic pressure, which is consistent with observations. On the other hand, the temperature of the incoming solar wind positively influences the H$^ $ and O$^ $ ion escape. This is attributed in part to the enhanced gyroradius of the particles, which allows them to penetrate deeper into the planet's atmosphere.

Wind tunnel testing and performance modeling of an atmospheric ion thruster

Abstract In this work a complete atmospheric electro–hydro-dynamic (EHD) thruster is tested in a subsonic wind tunnel, with the purpose of evaluating changes in performance due to simulated flight conditions and, for the first time, comparing them with a physical model of the drift region. An aerodynamic frame was designed to accommodate the electrodes inside the wind tunnel. Propulsive force and electrical measurements were conducted to assess performance exploiting dimensionless coefficients derived from one-dimensional theory. The results, on top of validating the theory, show how EHD thrusters can operate with a non-zero bulk velocity and highlight the importance of optimized frames and electrodes to enhance the capabilities of flying demonstrators. The test campaign revealed that the operating voltage envelope extends with increasing bulk velocity, leading to an increase in maximum thrust.

Ion Source Complementarity for Characterization of Complex Organic Mixtures Using Fourier Transform Mass Spectrometry: A Review.

Complex organic mixtures are found in many areas of research, such as energy, environment, health, planetology, and cultural heritage, to name but a few. However, due to their complex chemical composition, which holds an extensive potential of information at the molecular level, their molecular characterization is challenging. In mass spectrometry, the ionization step is the key step, as it determines which species will be detected. This review presents an overview of the main ionization sources employed to characterize these kinds of samples in Fourier transform mass spectrometry (FT-MS), namely electrospray (ESI), atmospheric pressure photoionization (APPI), atmospheric pressure chemical ionization (APCI), atmospheric pressure laser ionization (APLI), and (matrix-assisted) laser desorption ionization ((MA)LDI), and their complementarity in the characterization of complex organic mixtures. First, the ionization techniques are examined in the common direct introduction (DI) usage. Second, these approaches are discussed in the context of coupling chromatographic techniques such as gas chromatography, liquid chromatography, and supercritical fluid chromatography.

On the long-term stability of the association between foF2 and EUV solar proxies

Solar extreme ultraviolet (EUV) radiation is the main source of heating and ionization of the Earth's upper atmosphere, forcing most of this system's time variability, which in annual scales corresponds to the solar activity ∼11-year cycle. Due to the difficulties in obtaining solar EUV time series covering extended periods of time or during periods without measurements available, the use of solar EUV proxies became a solution. In the case of the ionosphere, and in particular the F2-layer critical frequency (foF2), in addition to the solar activity cycle variation, it may also exhibit the effect of long-term trend forcings, like the monotonous increasing greenhouse gas concentration since the industrial revolution. To accurately detect and measure this weak trend against the solar activity variability, it is crucial to account for the solar forced variation. Traditionally, it is modeled as a linear association between foF2 and a given solar EUV proxy. However, the stability of this association has become a controversial issue. It would be reasonable to assume, in turn, that if the ionospheric environment is undergoing a trend forced by a non-solar diver, like the greenhouse gas concentration increase, the relationship between foF2 and solar proxies may be affected, ceasing to be stable if this additional driver is not introduced in the modeled association. Using rolling regressions over the period 1960–2023 to analyze this stability, our results suggest that the issue may not only lie in the steady trend expected in foF2 from a non-solar source or the need to include terms in the simple linear regression commonly used, but also in the possible deviation of the different proxies from the 'true' EUV solar flux, which is the ultimate main driver of F2 region ionization, a deviation that has been intensifying over the last two decades. We assert that it is a deviation from the actual EUV behavior because the indices diverge from one another, something that should not occur if they all reflect the same solar EUV.

Development of an Ultrasonic Nebulization System for an Inverse Low Temperature Plasma Ionization Source.

An effective nebulization and evaporation of a liquid sample, like in liquid chromatography, mass spectrometry (LC-MS) couplings, is an essential requirement for the ionization of analyte molecules in the gas phase by, for example, atmospheric pressure chemical ionization (APCI) or the novel low temperature plasma (LTP)-based ion source. These LTP-based ion sources have recently gained interest in the field of atmospheric pressure ion sources, as they can cover a wide range of polarity and molecular mass. They can be used in combination with separation techniques like liquid chromatography or used as an ambient ion source. However, commercial nebulizer systems are of course not constructed to fit to home-built LTP-based ion sources, and this was one incentive to develop a new nebulization system. Instead of an atmospheric pressure chemical ionization (APCI) nebulizer, two commercial nebulizers were disassembled and remodeled to be used as nebulizing systems in an LC-MS setup using an LTP-based ion source. Based on these results, a novel nebulizer system was subsequently developed. To further improve the degree of ionization, cones to focus the LC eluent spray on the plasma region, heating applications, and auxiliary nitrogen gas for dispersion of the solvent droplets were implemented. The LOD that could be calculated via the rule of three resulted in an average of 2.0 μg/L for the APCI-nebulizer and 41 μg/L for the USN. Both could be reduced to 1.4 and 18 μg/L, respectively, by using a TPI-configuration instead of an iLTP. The linearity was equally good for both types of nebulization devices. The final nebulizer could also be operated with a high water content and flow rates higher than those of the two previous ones, indicating an important improvement step.

Atmospheric ion escape and solar wind deposition as a function of planetary radius

ABSTRACT We explore the ability of an unmagnetized planet to retain an atmosphere as a function of its radius. We use a particle-in-cell hybrid code to simulate the global plasma interaction of unmagnetized terrestrial planets at 1 au under average solar wind conditions. We vary the radius of the planet $(R_\mathrm{ p})$ from Mars-sized ($3390 \ \mathrm{km}$) to super-Earth-sized ($9390 \ \mathrm{km}$). We inject hydrogen and oxygen ion outflows from the ionosphere and quantify how the ion escape, recirculation, solar wind deposition, and net atmospheric mass flux vary as a function of planetary radius. We find that as the radius and the corresponding ionospheric outflow rate are varied, the fraction of outflowing $\mathrm{ H^+}$ that escapes remains at $15.5\pm 1.0{{\ \rm per\, cent}}$, while the rest recirculates back towards the planet. The fraction of produced $\mathrm{ O^+}$ that escapes from a Mars-sized planet is $27\pm 1{{\ \rm per\, cent}}$, and decreases to $7\pm 1{{\ \rm per\, cent}}$ for super-Earth, suggesting that smaller planets are less able to retain heavy ions. We find, however, that larger planets have lower solar wind deposition fractions because their bow shocks are at greater distances from the surface of the planet. The ionospheric outflow rate at which mass deposition is equal to mass escape is found to be proportional to $R_\mathrm{ p}^2$. Lastly, we propose that the bulk gyration of the solar wind at the induced magnetosphere can lead to differential escape trajectories of light and heavy ions.

Masks As a New Hotspot for Antibiotic Resistance Gene Spread: Reveal the Contribution of Atmospheric Pollutants and Potential Risks.

The consumption of disposable surgical masks (DSMs) considerably increased during the coronavirus pandemic in 2019. Herein, we explored the spread of antibiotic resistance genes (ARGs) and the potential risks of antibiotic resistant bacteria (ARB) on DSMs. At environmentally relevant concentrations, the conjugate transfer frequency (CTF) of ARGs increased by 1.34-2.37 folds by 20 μg/m3 of atmospheric water-soluble inorganic ions (WSIIs), and it increased by 2.62-2.86 folds by 80 ng/m3 of polycyclic aromatic hydrocarbons (PAHs). Total suspended particulates (TSP) further promoted the CTF in combination with WSIIs or PAHs. Under WSII and PAH exposure, gene expression levels related to oxidative stress, cell membrane, and the adenosine triphosphate (ATP) were upregulated. WSIIs predominantly induced cellular contact, while PAHs triggered ATP formation and membrane damage. Molecular dynamics simulations showed that WSIIs and PAHs reduced membrane lipid fluidity and increased membrane permeability through interactions with the phosphatidylcholine bilayer. DSM filtering performance decreased, and the CTF of ARGs increased with the wearing time. The gut simulator test showed that ARB disrupted the human gut microbial community and increased total ARG abundance but did not change the ARG abundance carried by ARB themselves. A mathematical model showed that long-term WSII and PAH exposure accelerated ARG dissemination in DSMs.

A Technique for Retrieving the Exospheric Number Density Distribution from Pickup Ion Ring Distributions

Ion pickup by the solar wind is ubiquitous in space plasma. Because pickup ions are originally produced by ionization of an exospheric neutral atmosphere, their measurements contain information on the exospheric neutral abundance. Here we established a method to retrieve exospheric number densities, by analyzing the ion velocity distribution functions of pickup ions measured by the Mars Atmosphere and Volatile EvolutioN spacecraft. We successfully retrieved exospheric oxygen density distributions at altitudes ranging from 1000 to 10,000 km around Mars except for the vicinity of the bow shock. This method can be applied to other space missions to study the upper atmosphere of planets, moons, and other small bodies in our solar system, where pickup ions exist.

Zonal‐Mean N2 and Ar Densities and Temperatures in Mars Thermosphere From MAVEN

AbstractMeasurements of Ar and N2 densities at 160–250 km altitude from the Mars Atmosphere and Volatile Evolution (MAVEN) Neutral Gas and Ion Mass Spectrometer (NGIMS) during February 2015–February 2023 are analyzed to provide a comprehensive analysis of their diurnal‐ and zonal‐mean (DZM) structures, and ZM (solar‐synchronous) diurnal (DW1) and semidiurnal (SW2) tides. After applying a solar flux trend correction, multi‐year binning and averaging with respect to longitude, local solar time (LST), latitude and Ls at each height results in the first full global picture of these components of the ZM thermosphere for a single climatological Mars year. The following new observational insights into Mars thermosphere are obtained: The DZM N2 latitude versus Ls (latvsLs) structures contain a prominent latitudinally‐symmetric annual component (∼±25%–35%) due to the eccentricity of Mars orbit around the Sun, and an antisymmetric component (∼±30%–45%) below about 190 km that is seasonally‐symmetric and thus consistent with the tilt of Mars rotation axis. Aperiodic deviations from these symmetries increase with height and are tentatively attributed to dissipation of waves originating in the lower atmosphere. DW1 and SW2 maximize around 200–220 km altitude, suggesting existence of an unknown dissipation mechanism at higher altitudes. The DZM, DW1 and SW2 components of Ar generally exceed those of N2 by factors of 1.4–2.5. The scale heights of Ar and N2 between 205 and 245 km are also employed to derive DZM exosphere temperatures, which reflect aperiodic ∼±15K deviations from the annual‐mean in the latvsLs frame.

Using dopants in the atmospheric pressure chemical ionization ion source to determine the site of protonation by ion mobility spectrometry.

Compounds like caffeine metabolites with more than one proton acceptor site can produce a mixture of isomeric protonated ions (protomers) in electrospray ionization and atmospheric pressure chemical ionization (APCI) ion sources. Discrimination between the protomers is of interest as the charge location influences ion structure and chemical and physical properties. Protonation of caffeine in an APCI ion source was studied using ion mobility spectrometry. The hydronium ions, H3O+(H2O)n, are the main reactant ions in the APCI ion source. Different dopant gases including NO2, NH3, and CH3NH2 were used to produce new reactant ions NO+, NH4 +, and CH3NH3 +, respectively. Density functional theory was employed to explain the experimental results and calculate the energies of the ionization reactions. The ion mobility spectrum of caffeine showed three peaks. In the presence of NO2 dopant and NO+ reactant ion, caffeine was ionized via charge transfer and formation of M+ ion. As NH3 and CH3NH2 are stronger bases than H2O, the reactant ions NH4 + and CH3NH3 + selectively protonated the more basic site of caffeine, that is, the imidazole nitrogen. Using these dopants, we could attribute the first ion mobility peak to M+ ion, the second peak to the protonation of caffeine at the carbonyl oxygen atom, and the third peak to the protonation of the imidazole nitrogen atom. The calculated collisional cross-sections of M+ and the protomers of caffeine confirmed the peaks' assignment. The criterion for the selection of an appropriate dopant is that its proton affinity (PA) should be between those of the proton acceptor sites of the molecule studied.

Combined UPLC-QqQ-MS/MS and AP-MALDI Mass Spectrometry Imaging Method for Phospholipidomics in Obese Mouse Kidneys: Alleviation by Feeding Sea Cucumber Phospholipids.

Sea cucumber phospholipids have ameliorative effects on various diseases related to lipid metabolism. However, it is unclear whether it can ameliorate obesity-associated glomerulopathy (ORG) induced by a high-fat diet (HFD). The present study applied UPLC-QqQ-MS/MS and atmospheric pressure matrix-assisted laser desorption ionization mass spectrometry imaging (AP-MALDI MSI) to investigate the effects of sea cucumber phospholipids, including plasmalogen PlsEtn and plasmanylcholine PakCho, on phospholipid profiles in the HFD-induced ORG mouse kidney. Quantitative analysis of 135 phospholipids revealed that PlsEtn and PakCho significantly modulated phospholipid levels. Notably, PlsEtn modulated kidney overall phospholipids better than PakCho. Imaging the "space-content" of 9 phospholipids indicated that HFD significantly increased phospholipid content within the renal cortex. Furthermore, PlsEtn and PakCho significantly decreased the expression of transport-related proteins CD36, while elevating the expression of fatty acid β-oxidation-related protein PPAR-α in the renal cortex. In conclusion, sea cucumber phospholipids reduced renal lipid accumulation, ameliorated renal damage, effectively regulated the content and distribution of renal phospholipids, and improved phospholipid homeostasis, exerting an anti-OGR effect.

Activity concentration of indoor radon and its short-lived progeny (218Po, 214Pb, and 214Po) and their effect on atmospheric ionization rate in Sana'a, Yemen

Exposure to ionizing radiation inside houses, especially radionuclides of radon and its progeny, poses serious health risks that can be exacerbated when inhaled as a result of interaction with human lung tissue. Also, air ionization is mainly due to these radionuclides. Therefore, accurate measurements of radon activity concentrations and its short-lived progeny are required to assess dose and environmental pollution and estimate ionization rates in indoor environments. For this purpose, we employed a previously tested and approved reliable method, following the three-count procedure. This method is based on airborne radon progeny sampling on polycarbonate membrane filters and alpha counting using a passive α-dosimetry technique with CR-39 detectors. The method also relies on a PC-based software we developed for solving mathematical equations and calculating all the necessary physical quantities. In this study, the concentrations of radon and individual short-lived radon progeny were measured in 20 houses in Sana'a, Yemen. Measurement conditions and meteorological variables were considered. The average activity concentrations of 222Rn, Equilibrium-Equivalent Concentration (EEC), 218Po, 214 Pb, and 214Po were 73.1 ± 6.0, 29.2 ± 2.4, 44.4 ± 3.6, 30.5 ± 2.5, and 23.2 ± 1.9 Bq.m-3, respectively. The calculated average unattached fractions f1(218Po), f2(214 Pb), and fp were found to be 0.24, 0.04, and 0.07 % respectively. The annual average values of ion-pair production rate caused by 222Rn and their progeny and air ion concentration, were 27.25 ions.cm-3s-1 and 1829 ions.cm-3 respectively. The annual effective dose was estimated to be 1.93 ± 0.16 mSv.y−1, well lower than the recommended 10 mSv.y−1.

Visualization of the Charging of Water Droplets Sprayed into Air.

Water droplets are spraying into air using air as a nebulizing gas, and the droplets pass between two parallel metal plates with opposite charges. A high-speed camera records droplet trajectories in the uniform electric field, providing visual evidence for the Lenard effect, that is, smaller droplets are negatively charged whereas larger droplets are positively charged. By analyzing the velocities of the droplets between the metal plates, the charges on the droplets can be estimated. Some key observations include: (1) localized electric fields with intensities on the order of 109 V/m are generated, and charges are expected to jump (micro-lightening) between a positively charged larger droplet and the negatively charged smaller droplet as they separate; (2) the strength of the electric field is sufficiently powerful to ionize gases surrounding the droplets; and (3) observations in an open-air mass spectrometer reveal the presence of ions such as N2+, O2+, NO+, and NO2+. These findings provide new insight into the origins of some atmospheric ions and have implications for understanding ionization processes in the atmosphere and chemical transformations in water droplets, advancing knowledge in the field of aerosol science and water microdroplet chemistry.

Search for Dark Matter Ionization on the Night Side of Jupiter with Cassini.

We present a new search for dark matter (DM) using planetary atmospheres. We point out that annihilating DM in planets can produce ionizing radiation, which can lead to excess production of ionospheric H_{3}^{+}. We apply this search strategy to the night side of Jupiter near the equator. The night side has zero solar irradiation, and low latitudes are sufficiently far from ionizing auroras, leading to a low-background search. We use Cassini data on ionospheric H_{3}^{+} emission collected three hours either side of Jovian midnight, during its flyby in 2000, and set novel constraints on the DM-nucleon scattering cross section down to about 10^{-38} cm^{2}. We also highlight that DM atmospheric ionization may be detected in Jovian exoplanets using future high-precision measurements of planetary spectra.

Measurements of Water-Soluble Ions in Particulate Matter 2.5 in Polish Rural Areas: Identifying Possible Sources

Limited data on rural Poland's atmospheric ion concentrations exists, with no publicly available monitoring data in urban areas. These knowledge gaps hinder the comparison of concentrations across environments and the identification of their sources. This study examines water-soluble ions across five rural locations in Poland over four years to investigate their concentrations and sources in the atmosphere. This study explores aerosol origins, performing a four-year correlation analysis across five locations to reveal ion relationships. Notably, sulfate (SO₄2⁻), nitrate (NO₃⁻), and ammonium (NH₄⁺) exhibit significant correlations ranging from 0.3 to 0.8, suggesting a common pollution source in all analyzed rural locations. Interestingly, magnesium (Mg2⁺) and sodium (Na⁺) in two locations demonstrated a strong correlation, ranging between 0.4 and 0.9, suggesting the influence of sea spray on these sites. Principal component analysis is used to investigate the factors influencing ion concentrations, revealing distinctive patterns for each location and explaining the total variances ranging from 74.9% to 84.8%. This underscores the significance of geographical and environmental factors. The study's novelty lies in its thorough and long-term analysis of water-soluble ion concentrations across rural Poland, providing an extensive dataset for the region. The study fills a data gap on rural pollution sources and reveals consistent ion patterns across different sites and seasons. The findings emphasize geographical and environmental impacts on aerosol composition and suggest common pollution sources for all areas. This research encourages further investigations into the stability and origins of ions in rural environments, providing valuable insights for local and broader atmospheric studies.

Mass Spectrometry Imaging of Coniine and Other Hemlock Alkaloids after On-Tissue Derivatization Reveals Distinct Alkaloid Distributions in the Plant.

Specialized metabolites play important roles in plants and can, for example, protect plants from predators or pathogens. Alkaloids, due to their pronounced biological activity on higher animals, are one of the most intriguing groups of specialized metabolites, and many of them are known as plant defense compounds. Poison hemlock, Conium maculatum, is well-known for its high content of piperidine alkaloids, of which coniine is the most famous. The distribution, localization, and diversity of these compounds in C. maculatum tissues have not yet been studied in detail. The hemlock alkaloids are low molecular weight compounds with relatively high volatility. They are thus difficult to analyze on-tissue by MALDI mass spectrometry imaging due to delocalization, which occurs even when using an atmospheric pressure ion source. In this manuscript, we describe an on-tissue derivatization method that allows the subsequent determination of the spatial distribution of hemlock alkaloids in different plant tissues by mass spectrometry imaging. Coniferyl aldehyde was found to be a suitable reagent for derivatization of the secondary amine alkaloids. The imaging analysis revealed that even chemically closely related hemlock alkaloids are discretely distributed in different plant tissues. Additionally, we detected a yet undescribed hemlock alkaloid in Conium maculatum seeds.

Role of the Abcg2 transporter in plasma, milk, and tissue levels of the anthelmintic monepantel in mice

Breast cancer resistance protein/ATP-binding cassette subfamily G2 (BCRP/ABCG2) is an ATP-binding cassette efflux (ABC) transporter expressed in the apical membrane of cells in tissues, such as the liver, intestine, kidney, testis, brain, and mammary gland. It is involved in xenobiotic pharmacokinetics, potentially affecting the efficacy and toxicity of many drugs. In this study, the role of ABCG2 in parasiticide monepantel (MNP) and its primary metabolite, monepantel sulfone (MNPSO2)'s systemic distribution and excretion in milk, was tested using female and male wild‐type and Abcg2−/− mice. Liquid chromatography coupled with a tandem mass spectrometer (LC-MS/MS) was used for the analysis in a 10-min run time using positive-mode atmospheric pressure electrospray ionization (ESI+) and multiple reaction monitoring (MRM) scanning. For the primary metabolite tested, milk concentrations were 1.8-fold higher in wild-type mice than Abcg2−/− female lactating mice (P = 0.042) after intravenous administration of MNP. Finally, despite the lack of a difference between groups, we investigated potential differences in MNP and MNPSO2's plasma and tissue accumulation levels between wild‐type and Abcg2−/− male mice. In this study, we demonstrated that MNPSO2 milk levels were affected by Abcg2, with potential pharmacological and toxicological consequences, contributing to the undesirable xenobiotic residues in milk.