Atlantic Forest Research Articles

Host plants of the weevil genus Heilipus Germar, 1824 (Coleoptera, Curculionidae, Molytinae, Molytini, Hylobiina)

Heilipus Germar, 1824 (Coleoptera, Curculionidae, Molytinae, Molytini, Hylobiina) is a largely understudied tropical lineage of weevils with 90 described species distributed from the southern United States to northern Argentina. Several of its species cause damage to avocado (Persea americana Mill.; Lauraceae Juss.) crops in the Americas. Apart from the species of economic importance, there is a paucity of information regarding the host plants of species of Heilipus. This study presents a comprehensive overview of the current knowledge on the habits and host plants of this group of weevils, based on a literature review and the examination of label data associated with entomological material in museums and scientific collections. The larval stages of 24 Heilipus species are borers of either seeds or trunks of 38 plant species belonging to 13 genera: 33 species (12 genera) from the family Lauraceae and five species (one genus) from the family Annonaceae Juss. Four new host plant records and the first identification of habits and host plants for H. albovenosus (Champion), H. empiricus (Pascoe), H. fassli (Voss) and H. mirus (Pascoe) are provided. The biology and host plants of the seed and trunk borers are discussed, as well as the oligophagous feeding behaviour exhibited by these two feeding guilds. Seed-boring Heilipus species constitute one of the primary groups of pre-dispersal seed predators of the family Lauraceae in the Americas. Furthermore, the high diversity of Heilipus and its host plants in the Brazilian Atlantic Forest suggests that this area may be a possible centre of origin for these weevils.

A taxonomic notes on Mouriri (Olisbeoideae, Melastomataceae): Clarifying two little-known species from the Brazilian Atlantic Forest, with three new synonyms

Based on new samples from the Atlantic Forest between northern São Paulo and southern Bahia, Brazil, we clarify taxonomic relationships in two little-known species of Mouriri. As a result, we synonymize M. megasperma and M. sellowiana under M. arborea, and M. bahiensis under M. regeliana. We designate a lectotype for M. sellowiana, as the holotype was destroyed during World War II. In addition, we present updated morphological descriptions, data on geographic distribution, conservation status assignments, illustrations, and comments on taxonomic and nomenclatural aspects of M. arborea and M. regeliana. Photographs based on fresh material of M. arborea are presented.

Selection of Atlantic Forest species in South Bahia, Brazil

The study aimed to evaluate the growth potential of several Atlantic Forest species in South Bahia, displayed in high dense grid and select the best trees through selection indexes. The experiment was carried out in the Comissão Executiva do Plano da Lavoura Cacaueira (CEPLAC) located in the South of Bahia state, Brazil. A total of 44 Atlantic Forest species were tested with three replications with plot containing 36 individuals that were arranged in 2 × 2 m spacing. Experimental parameters indicated high variability among species to high density spacing, which is favorable to commercial pure stands practiced in the forestry sector. Statistical selection of native species was evaluated using selection indices based on volumetric production and survival traits. These indices allowed select species by their adaptation into the experimental region. The top two ranked species were Pterygota brasiliensis Allemão (Folheiro ou pau-rei) and Plathymenia reticulata Benth. (Vinhático), which adapted well to a tropical, humid, and rainy weather, such as the climate in South Bahia, Brazil. From this study, it was possible to subsidize the selection of ideal species and individuals for seed collection, indication of materials to assemble breeding populations, cloning, and orientate future studies in South Bahia state, Brazil.

Agricultural expansion over fragments of atlantic rainforest in northeast brazil and its impacts on lichens

Agricultural expansion over fragments of atlantic rainforest in northeast brazil and its impacts on lichens

Historical biogeography of Vellozia (Velloziaceae) reveals range expansion in South American mountaintops after climatic cooling events and increased diversification rates after the occupation of Southern Espinhaço Province

Abstract The campos rupestres and the Brazilian Atlantic Forest Inselbergs (BAFI) are highly diverse vegetation types that grow on mountaintops of eastern Brazil and show outstanding levels of endemism. The plant family Velloziaceae is an iconic element of these vegetations, with the genus Vellozia, being exceptionally abundant in both these vegetations. In this study, we use Vellozia as a model to address three main questions: (i) What was the distribution of Vellozia’s most recent common ancestor? (ii) Did the range expansions of Vellozia occur during periods characterized by global cooling? (iii) When did Vellozia colonize the different South American highlands they occupy nowadays? To address these questions, we reconstructed the phylogeny of Vellozia using sequences of four molecular markers analysed using Bayesian and maximum likelihood inferences. We used the resulting phylogeny to reconstruct the ancestral distribution of Vellozia using the DEC model. Our findings indicate that Vellozia originated and subsequently diversified in the Oligocene, when the genus was broadly distributed through the Andes, BAFI, Cerrado, Caatinga, and the Chapada Diamantina, suggesting that the Cerrado may acted as a corridor between the Andes and eastern mountaintop vegetations. Vellozia subsequently occupied the southern Espinhaço during the Early Miocene, which was followed by increased diversification rates and several range expansions, especially after the Middle-Miocene Climatic Optimum, when cooler and drier periods allowed the expansion of open environments and the retraction of forests, allowing Vellozia to expand their distribution. These results highlight the unique evolutionary history of Vellozia and the importance of climatic cooling for the expansion of the genus.

Importance of habit and environmental characteristics in shaping patterns of richness and range size of ferns and lycophytes in the Atlantic Forest.

Ferns and lycophytes, the two spore-bearing lineages of vascular plants, share a unique life cycle, and because of several morpho-ecophysiological similarities, are usually investigated as a unit, but they may have distinct ecological and environmental responses. Understanding the diversity and distribution patterns of ferns and lycophytes separately is essential for designing effective conservation strategies. We assessed species richness and range sizes using range-diversity analyses and investigated environmental (climatic, edaphic) and ecological (endemism, habit diversity, specialization) predictors of these range and richness metrics using generalized linear models. The central region of the Atlantic Forest primarily contained areas with fern and lycophyte species with high richness and small ranges; the northern and southern extremes of the forest had species with low richness and larger ranges. Fern richness was associated with a combination of environmental and ecological variables; lycophyte richness was associated only with ecological variables. Range sizes were positively related to habit diversity for both ferns and lycophytes, but the effect of environmental variables differed between the two lineages. Critical areas were primarily not within protected areas. Fern range sizes and richness were influenced by a combination of ecological and environmental factors such as temperature, precipitation, and soil variables; only ecological variables influenced lycophyte richness and range sizes. The effects of these factors must be considered separately for the two lineages due to their different responses to stressors. Conservation efforts should increase in the species-rich-low-range areas that are outside protected areas to protect these low-range species.

A fourth species of Chrysobalanus (Chrysobalanaceae): an endemic tree in the Atlantic Forest

A fourth species of Chrysobalanus (Chrysobalanaceae): an endemic tree in the Atlantic Forest

Stable Isotopes Analysis of Black Lion Tamarins Reveals Increasing Arthropod Consumption When Fruit Productivity Decreases in Forest Fragments.

Given the cryptic and elusive nature of prey consumption, quantifying its contribution to the diet of free-ranging primates using behavioral methods is challenging. In this context, the use of carbon and nitrogen-stable isotopes represents a promising alternative approach. Here, we used stable isotope analysis to estimate the proportion of arthropods and fruits in the diet of black lion tamarins (Leontopithecus chrysopygus), an endangered primate endemic to the Brazilian Atlantic Forest. To do so, we ran stable isotope mixing models using isotopic data from hair samples of black lion tamarins living in six forest fragments showing different levels of habitat quality. Furthermore, we ran linear mixed models to assess the influence of habitat quality-fruit productivity (estimated by tree total basal area) and arthropod biomass - and individual characteristics (sex and body mass) on tamarins' δ15N values (a proxy for trophic position). Our results revealed that arthropods contributed more to black lion tamarins' diet than reported in previous behavioral studies, suggesting that behavioral observations may considerably underestimate the importance of arthropodivory in the diet of arboreal primates. The degree of arthropodivory and frugivory was similar within groups, in line with the strong group cohesion and synchronization of feeding behaviors of this species and supporting the role of site-specific habitat characteristics on dietary choice. Arthropod consumption was higher in areas with lower fruit productivity and did not increase when arthropod biomass increased, suggesting that fruits represent a limiting but preferred resource for this species. These results demonstrate the dietary plasticity of black lion tamarins in areas of lower forest quality, where they manage to compensate low fruit productivity by shifting to a diet richer in arthropods. Considering that this species occurs within a highly fragmented landscape, preserving and protecting small forest patches remains crucial for the conservation of this species.

Palynotaxonomy of Neotropical species of Paullinia L. (Sapindaceae)

Paullinia is the second-largest genus within the Sapindaceae, with approximately 200 species. It predominantly comprises lianas that inhabit humid forests, mainly the Amazon and Atlantic forests in Brazil. This study aimed to characterize the pollen morphology of 60 species of Paullinia, examining the potential of pollen characters as morphological markers to support the systematics of the genus. The pollen material was obtained from exsiccatae of flower buds from major herbaria. Pollen grains were acetolyzed, measured, described, and illustrated using light microscopy. Scanning electron microscopy was used for accurate descriptions of surface features. The analyzed characters included pollen shape, size, aperture type and number, and exine ornamentation. The similarity of pollen characters between species was analyzed using hierarchical cluster analysis and principal component analysis. The results showed that pollen grains of Paullinia species are shed in monads, heteropolar in most species and isopolar in some others, medium or large in size, oblate or suboblate, and 3- or 3-4-porate, with perforate sexine ornamentation in most species and microreticulate in some others. Multivariate analyses revealed that pollen morphology is informative and holds taxonomic potential, enabling grouping based on the similarity of the unique pollen characters of the genus. Species dispersion on the PCA biplot showed that the five clusters formed by HCA were robust, suggesting that quantitative and qualitative pollen characters exhibit consistency with regard to the similarity species. The palynological findings presented here contribute to enhancing our understanding of the morphological characters, aiding in the taxonomy and systematics of the genus.

Hidden diversity in anthropophilic sand flies of the Monticola Series (Diptera, Psychodidae)

The Monticola series comprises two anthropophilic and widely distributed species in Brazil: Pintomyia (Pifanomyia) monticola (Costa Lima, 1932) and Pintomyia (Pifanomyia) misionensis (Castro, 1959). They mainly occur in the Atlantic Rainforest, and it is known that Pi. monticola comprises at least two well-structured genetic lineages regarding a fragment of the cytochrome c oxidase subunit I (COI) gene. Here, we aim to elucidate the taxonomic status of this group using integrative taxonomy tools. Collections were performed in nine localities of four Brazilian states, and COI fragments were sequenced and merged with publicly available data. Several single-locus species delimitation algorithms, genetic distance metrics, phylogenetic trees, and haplotype networks were used to uncover cryptic diversity and population structure within Pi. monticola and Pi. misionensis. The resulting genetic clusters were then tested for morphological differences through linear and geometric morphometry of several characters. We analyzed 152 COI sequences, comprising 48 haplotypes. The maximum intraspecific p distances were 8.21% (mean 4.17%) and 9.12% (mean 4.4%) for Pi. monticola and Pi. misionensis, respectively, while interspecific ones ranged from 10.94 to 14.09% (mean 12.33%). Phylogenetic gene trees showed well-supported clades for both species, with clear structuring patterns within them. Species-delimitation algorithms split our dataset into at least three putative species for each taxon. Moreover, population structure analysis showed a strong correlation between Atlantic Forest areas of endemism as sources of molecular variation in Pi. monticola. Morphometric analyses were significant for wing shape variation and some linear measurements (mainly of the head) when comparing specimens of different genetic clusters for both taxa. These results indicate strong genetic structuring of Monticola series species, confirmed by morphometry, indicating two possible cryptic species complexes.

Landscape degradation drives metal bioaccumulation in bats from Atlantic Forest cacao region, Brazil.

Agricultural landscapes worldwide are heavily sprayed with agrochemicals to increase crop productivity. These agrochemicals release bio-accumulative pollutants such as heavy metals that often persist in the environment with harmful impacts on biota. In a prime endangered Atlantic Forest biome, in Bahia, Brazil, agroforestry of cacao (Theobroma cacao) provides a livelihood for small farmers and suitable habitats for forest species. However, landscape transformation to pasture and monoculture expose vulnerable communities to scarcely evaluated pollutants with unknown effects on the health of humans and animals. We assessed the bioaccumulation of manganese (Mn), lead (Pb), and copper (Cu) by analyzing hair samples of 326 bats representing 28 species across 15 cacao agroforestry and 2 forest remnants. Bats from regions heavily disturbed by pastures and monocultures showed higher levels of Pb (41.20µg/g) and Mn (0.44µg/g) compared to those from areas where forest or cacao agroforestry dominates the landscape. Local grassland covers increased Pb bioaccumulation, while forest cover reduced it. Cacao agroforestry appeared to increase Cu exposure, likely due to fungicide use. This study pioneers the evaluation of heavy metal accumulation in bats inhabiting cacao agroforestry and Atlantic Forest remnants, highlighting the need for sustainable agricultural practices to protect wildlife and ecosystem health.

Epiphytic orchids show specificity for phorophyte and strata on palm trees in a restinga area in the Brazilian Atlantic Forest

Epiphytic orchids show specificity for phorophyte and strata on palm trees in a restinga area in the Brazilian Atlantic Forest

De Olho na Mata: monitoring Atlantic Forests with drones and few-shot learning

De Olho na Mata: monitoring Atlantic Forests with drones and few-shot learning

Genome Dynamics of Adaptation and Introgression in Neotropical Palms

ABSTRACTAimThe combined influences of demographic dynamics and gene flow on local adaptation in plants is still poorly understood. Here, we used a genome scan approach on three closely related Neotropical palms, Acrocomia aculeata, A. intumescens and A. totai, to identify the evolutionary processes generating shared and lineage‐specific patterns of differentiation and selection across the genomic landscape.LocationAmazonia, Atlantic Forest, Cerrado and Caatinga ecoregions of Brazil.TaxaArecaceae.MethodsWe used target sequence capture and analysis of climatic correlation, detection of selective sweeps, balancing selection, and spatial and non‐spatial models to identify signatures of natural selection and admixture. We also determined temporal dynamics in spatial distribution and demographic changes.ResultsWe found a higher number of lineage‐specific than shared adaptive sites (SNPs) and no evidence of selective sweeps in shared genes, suggesting lineage specific natural selection across species. Further, evidence of balancing selection in several genes was also identified in the three species. Niche‐based and coalescent models suggest that shifts in spatial range during the Quaternary caused overlapping distributions between species, leading to hybridisation between parapatric localities.Main ConclusionsInterspecific hybridisation may have spread both neutral and adaptive SNPs, which may explain the shared adaptive genes between species. Taken together, we show how genomic adaptation can occur despite introgression, through evolutionary processes that likely drive similar patterns of adaptation in other organisms.

Uncovering the rich amphibian fauna of two semideciduous forest fragments in southwestern Bahia, Brazil.

Fauna inventories reduce biodiversity knowledge gaps by providing comprehensive data on species distribution, richness, and abundance. Furthermore, they identify undocumented species and enhance understanding of ecosystem dynamics and conservation needs. The richness and abundance of amphibian species were studied in two Semideciduous Seasonal Forest areas in the municipalities of Potiraguá (Serra Azul) and Itarantim (Serra do Mandim) in southwestern Bahia, Brazil. Active visual and acoustic surveys were conducted in 24 forest interior transects, two stream transects, and two permanent ponds investigated in the study area. Opportunistic encounters during team movements were also recorded. The richness was 46 amphibian species distributed in 14 families and 26 genera. Approximately half of the species were shared between the two areas, while 11 species were exclusive to Serra Azul and another nine were found only in Serra do Mandim. Cluster analysis for 42 locations in Atlantic Forest, Caatinga, and Cerrado, in a presence/absence matrix with 216 species, revealed that the composition of the amphibians found in Serra do Mandim and Serra Azul is similar to other sampled locations in the northeastern region of Minas Gerais, close to the study site, which are considered transitional between the Atlantic Forest and the Caatinga. Our results demonstrate that the remaining forest fragments in the region, although small and isolated, still sustain a high richness of amphibians with species restricted to the Atlantic Forest and Bahia, such as Bahiusbilineatus, Ololygonstrigilata, Aplastodiscusweygoldti and Vitreoranaeurygnatha, and others considered typical of the Caatinga, such as Leptodactylustroglodytes and Physalaemuscicada. Additionally, we sampled potential new species, filled occurrence gaps, and expanded the geographical range of Pseudisfusca.

Adaptation to different temperatures results in wing size divergence of the invading species Drosophila nasuta (Diptera: Drosophilidae) in Brazil.

Invasive species threaten biodiversity on a global scale. The success of invasions depends on the species' adaptation to the different environmental conditions of new territories. Studies show that invasive insects present evolutionary changes in wing morphology in areas they are introduced to in response to abiotic conditions. In the last decade, the Asian Drosophila nasuta fly invaded and spread widely throughout Brazil. This insect has preferences for conserved environments and is related to the likely reduction in the abundance of native drosophilids in the Atlantic Forest. Ecological niche modelling analyses showed that rainfall and temperature are the main factors which delimit the geographic distribution of this species. Herein, we verified the existence of significant differences in the wing sizes of D. nasuta in Brazil and evaluated the influence of abiotic factors (rainfall and temperature) on the observed patterns. We conducted 11 measurements on the right-side wings of 240 D. nasuta males collected in the Amazon Forest, Caatinga, Cerrado and Atlantic Forest. Statistical analyses revealed the existence of two groups: one with larger wings, which brought together samples from locations with the lowest temperatures; and one with smaller wings, which corresponded to places with a hotter climate. One explanation for this result is the fact that large wings favour greater heat capture by flies in colder climates, increasing their survival chances in these environments. These rapid evolutionary changes in D. nasuta in this first decade of invasion in Brazil reveal the enormous adaptive potential of this species in this megadiverse country.

New record of Youngomyia pouteriae Maia, 2001 (Diptera, Cecidomyiidae) in Pouteria caimito (Ruiz & Pav.) Radlk. (Sapotaceae) in the Peruvian Amazon

We report for the first time cylindrical galls of Youngomyia pouteriae Maia, 2001 (Diptera, Cecidomyiidae) on Pouteria caimito (Ruiz & Pav.) Radlk. (Sapotaceae) in Peru. Youngomyia pouteriae were exclusively found in the Atlantic Forest biome, in restinga areas of Rio de Janeiro state, Brazil. In this study, the geographic distribution of this galling species is extended to the western Amazon lowland rainforest in Peru.

Atlantic Forest Regeneration Dynamics Following Human Disturbance Cessation in Brazil

The Brazilian Atlantic Forest (BAF) is one of the most important biodiversity hotspots and species-rich ecosystems globally. Due to human activities, it has been significantly reduced and fragmented. This study examined both biotic (floristic composition, diversity, and structure) and abiotic (topographic and soil) factors in BAF fragments undergoing varying levels and durations of human disturbance cessation: approximately 20 years (20 y), ~30 years (30 y), and over 40 years (>40 y). We aimed to understand the recovery dynamics of floristic composition, diversity, and structure in BAF fragments in relation to abiotic factors. Several statistical tools were employed to examine similarities/differences and relationships. Forests of the 30 y group exhibit significantly greater homogeneity in terms of floristic composition, while forests of the 20 y group are characterized by lower species abundance and diversity. The floristic composition was primarily influenced by soil features and the time of disturbance. Under “Environmental Protection Areas”, soil–vegetation recovery can occur more swiftly than usually observed for BAF. A significant BAF recovery was observed approximately 40 years after the end of human disturbance. A partial recovery featured 30 y disturbed areas, while in 20 y forests, recovery is in its early stages. Human-disturbed BAF can gradually rebound when effective management practices are implemented.

Araçaúna (Psidium myrtoides) Flour: Sequential Extraction with Supercritical Carbon Dioxide and Pressurized Fluids

Araçaúna (Psidium myrtoides) is a native fruit from the Brazilian Atlantic Forest. It was studied for the proximate composition of its flour and sequential extraction using supercritical fluid extraction (SFE) in the first stage with CO2 and pressurized liquid extraction (PLE) with ethanol and water in the second and third stages, respectively, at 50°C and 60°C. The proximate analysis revealed essential information about the flour regarding the content of protein, carbohydrates, lipid, ash and moisture. The sequential extraction at 60°C resulted in high yields (42.22%). The first stage using SFE captured the volatile fraction, notably 1,4-diethylbenzene, which corresponds to 53.70% of the total volatile compounds identified in the araçaúna. Comparing the second and third stages, the third stage with water at 50°C produced the highest levels of phenolic compounds (33.06±2.26mg GAE/g extract), total monomeric anthocyanins (3.91±2.10mg C3G/g extract), antioxidant activity by ORAC (415.13±13.66 µmol TE/g extract) and FRAP (747.16±18.49 µmol TE/g extract), and cyanidin-3-glucoside as the predominant compound (4.83% w/w).

First molecular detection of adenoviruses in bats from an urban Atlantic Forest in Rio de Janeiro, Brazil

Bats comprise one of the most diverse and abundant groups of mammals in the world and host a significant viral diversity with zoonotic potential. Bat adenoviruses (bat AdVs), members of the family Adenoviridae, have been detected in several bat species, suggesting that bats are natural reservoirs. Here, faeces and rectal/anal-swabs were collected from 321 bats of an urban Atlantic Forest remnant from Rio de Janeiro, during 2019–2022, and screened for bat AdV nucleic acid with PCR. The positivity of bat AdVs was 3.7 % (12/321). Twelve individuals of four bat species were infected: Artibeus lituratus (66.7 %; 8/12), Desmodus rotundus (8.3 %; 1/12), Platyrrhinus lineatus (16.7 %; 2/12), and Sturnira lilium (8.3 %; 1/12). Phylogenetic analysis based on nucleotide and amino acid sequences showed that the detected bat AdVs clustered into four clades corresponding to the host species, identifying the presence of two potentially new bat adenoviruses. This is the first report of bat AdV detected in Platyrrhinus lineatus.