The etiology of running related injuries is still not fully understood. It is supposed to be multifactorial and asymmetry has been proposed as a contributing factor. Asymmetry might occur or increase due to exercise induced fatigue. Most research on asymmetry has been performed within the limitations of the laboratory setting. Inertial Magnetic Measurement Units (IMU’s) enable these measurements in the sport-specific setting PURPOSE: To investigate the effects of a 20 minute threshold run on an athletic track on asymmetry as indicated by 3D Center of Mass (COM) movement. METHODS: Five trained and injury free male runners (Age=29.8+/-3.0 yr, height=183.6+/-1.3 cm, weight 78.8+/-9.5 kg) participated in this study. Runners were equipped with an IMU sampling at 100 HZ, placed at the sacrum. 3D COM movement was calculated from gyroscope accelero- and magnetometer IMU data. A 20 min. threshold run was performed at Critical Velocity, based on the runners’ 10 km seasonal, best to impose exercise induced fatigue. Two stages were defined at the beginning (S1) and end (S2) of the run where mean COM displacements for the stride cycles of the both legs were calculated over 75 strides. An ANOVA was used to test for significant changes in COM displacement within a runner. RESULTS: COM displacement changed significantly over the two stages for all subjects for the right leg but only in two subjects for the left leg, indicating an increase in asymmetry in three runners.Table 1: COM displacements during the stride cycle of the right and left leg with their significance.CONCLUSIONS: Asymmetry, as defined by 3D Center of Mass (COM) movement, increased in three of the five runners during a 20 min threshold run at constant velocity. An increase in asymmetry during a run might indicate different joint loading and subsequently additional muscle work which eventually could lead to injuries.