Atemoya Fruit Research Articles

Protective Effects of Annona Atemoya Extracts on Inflammation, Oxidative Stress, and Renal Function in Cadmium-Induced Nephrotoxicity in Wistar Rats.

Cadmium (Cd), a highly toxic heavy metal from agricultural activities, and its exposure can lead to impaired renal function by increasing reactive oxygen species. The atemoya fruit is known for its high phenolic and antioxidant compounds. This study aimed to evaluate the effects of atemoya extracts on renal function, oxidative stress parameters, and inflammatory biomarkers in a cadmium-induced nephrotoxicity model. Three aqueous extracts were prepared from different parts of the atemoya fruit: seeds, peel, and pulp. Twenty-five male Wistar rats were allocated into four groups: control, seed, peel, and pulp extracts at 2 g/kg for 25 days. All treatment groups administered intraperitoneal injections of cadmium chloride (CdCl2) (2 mg/kg) to induce renal damage. The cadmium-treated groups showed decreased creatinine clearance, SOD, CAT, and GPx activities (p < 0.05) and increased serum levels of TNF-α and IL-6 compared to the control group (p < 0.05). The treatment with seed, peel, and pulp extracts increased creatinine clearance (p < 0.05), increased SOD, CAT, and GPx activities (p < 0.05), and reduced serum levels of TNF-α and IL-6 compared to the Cd group (p < 0.05). This study supports the use of atemoya as a promising candidate for mitigating nephrotoxicity and highlights the importance of its antioxidant and anti-inflammatory properties in renal health.

Postharvest quality and prolong storage time of atemoya (Annona squamosa x A. cherimola hybrids) fruit: coating with D-limonene nanoemulsion edible film

ABSTRACT Atemoya (Annona squamosa x A. cherimola hybrid) is a typical climacteric fruit that has a high respiration rate after harvest, short storage life, and is highly susceptible to fruit cracking and rot. Therefore, this study was intended to apply D-limonene nanoemulsion coating on fruit to prolong the storage life, reduce spoilage, and facilitate the expansion of the market. Green mature atemoya fruit coated with different concentrations of D-limonene nanoemulsion (0.25%, 0.5%, 1.0%, 1.5%, and control) was stored in a 14°C refrigerator for relevant analysis and investigation. The results showed that the use of 0.25% D-limonene nanoemulsion inhibited the browning on the fruit surface, and reduced physiological metabolisms. The browning phenomenon on the fruit surface increased as the D-limonene concentration ratio increases. Since the high concentration caused damage to the peel cells, 0.25% D-limonene was chosen as optimum for atemoya coating. The fruit of atemoya maintained green peel color (L × 28.6, a × 6.63, b × 12.3) after storage at 14°C for 21 days with 0.25% D-limonene nanoemulsion coating. At the same time, after 0.2% D-limonene nanoemulsion coating, the weight loss rate was about % less than that of the control group (0.25% treatment was 11.3%; control was 14.2%) and high pectin content (0.25% treatment was 16.9 mg AGA g−1AIS−1; control was 9.89 mg AGA g−1AIS−1). Coating of D-limonene nanoemulsion can reduce the physiological response of atemoya fruits after harvesting, prolong the storage time, and increase the feasibility of their export. From the experimental results, D-limonene showed the potential to be widely used in fruit preservation agents in the future.

Modification of Sugar Profile and Ripening in Atemoya (Annona × atemoya Mabb.) Fruits through Copper Hydroxide Application

Atemoya (Annona × atemoya Mabb.), a climacteric fruit of the Annonaceae family, is becoming increasingly popular due to its organoleptic and nutritional properties. Anthracnose, a fungus of the Colletotrichum genus, is one of the most serious diseases in orchards, causing significant damage if not controlled, so producers use phytosanitary products. The current study sought to investigate the quality of atemoya fruits after harvest in an orchard with anthracnose controlled by Cu(OH)2 application: T1-no Cu(OH)2, T2-7.8 mL Cu(OH)2 L1 divided into two applications, T3-15.6 mL Cu(OH)2 L1 divided into four, T4-8.0 mL Cu(OH)2 L1 divided into eight, and T5-13.0 mL Cu(OH)2 L1 divided into thirteen applications. The sugar profile of fruits was examined, as well as MDA, H2O2, and quality parameters such as pH, mass, soluble solids, titratable acidity, and maturation index. MDA, such as H2O2, can function as a signal molecule. Eight applications of 1.0 mL L-1 Cu(OH)2 resulted in increased concentrations of H2O2 and MDA, signal molecules involved in sugar modification profiles such as glucose, fructose, and trehalose. It also had a high titratable acidity, a lower maturation index, better fruit quality, and a longer shelf life.

Dinâmica da infestação de brocas em frutos de atemoieira em duas épocas de produção

Abstract Cerconota anonella (fruit borer) and Bephratelloides pomorum (seed borer) are the main pests of Annonaceae and knowing their population dynamics is essential to establish their management in orchards of these crops. The objective of this study was to evaluate the influence of native vegetation on the population of fruit and seed borers in two production seasons in an atemoya orchard in the semiarid region. The study was carried out in a commercial atemoya orchard in Janaúba, MG. The experimental area was divided into two parcels and, in each of them, the distance from the experimental plots to the surrounding vegetation, of approximately 10m, 33m, 57m and 81m, was recorded. The sampling of insects was carried out at different periods, spring and summer, through the collection, every two weeks, of atemoya fruits containing attack signs by the borers. The intensity of infestation of each borer species was determined. The intensity of borer infestation varies between seasons, with fruit borer infestation in summer and seed borer infestation in spring. The distance from the orchard to the adjacent vegetation does not interfere with the infestation intensity of fruit and seed borers.

In Vitro and In Vivo Assessment of Atemoya Fruit (Annona atemoya) for Food-Drug Interactions.

Atemoya (Annona atemoya) is increasingly being consumed worldwide because of its pleasant taste. However, only limited information is available concerning possible atemoya-drug interactions. In the present study, the issue of whether atemoya shows food-drug interactions with substrate drugs of the major drug-metabolizing cytochrome P450s (i.e., CYP1A2, CYP2C9, and CYP3A) is addressed. The ability of atemoya juice to inhibit the activities of phenacetin O-deethylase (CYP1A2), diclofenac 4'-hydroxylase (CYP2C9), and midazolam 1'-hydroxylase (CYP3A) was examined in vitro using human and rat liver microsomes. The in vivo pharmacokinetics of phenacetin and metabolites derived from it in rats when atemoya juice or fluvoxamine (a CYP1A2 inhibitor) was preadministered were also investigated. Atemoya juice significantly inhibited CYP1A2 activity in human liver microsomes, but not the activities of CYP2C9 and CYP3A. In spite of this inhibition, preadministration of atemoya had no effect on the pharmacokinetics of phenacetin, a CYP1A2 substrate, in rats. Meanwhile, preadministration of fluvoxamine significantly extended the time needed for the elimination of phenacetin, possibly due to the inhibition of CYP1A2. This suggests that the intake of an excess amount of atemoya juice is necessary to cause a change in the pharmacokinetics of phenacetin when the IC50 values for CYP1A2 inhibition by atemoya and fluvoxamine are taken into account. The results indicate that a daily intake of atemoya would not change the pharmacokinetics of CYP1A2 substrates such as phenacetin as well as CYP2C9- and CYP3A-substrate drugs.

Bioactivity of atemoya fruits and by-products

The atemoya fruit (Annona cherimoya Mill x Annona squamosa L.) has attracted market interest due to its organoleptic and nutritional characteristics. However, little is known about its bioactive compounds. Therefore, this study aimed to evaluate the free and bound phenolic profiles, and the antioxidant and antimicrobial activities of the pulp, peels, and seeds of atemoya. The fatty acids composition and tocopherols of atemoya seed oil were also studied. Ten phenolic compounds were observed in the different parts of atemoya: 4 in the free form (catechin, rutin, epicatechin and chlorogenic acid) and 6 in the bound form (p-coumaric, 3,4-dihydroxybenzoic, vanillic, caffeic and ferulic acids, and quercetin). Overall, the peels had the highest concentration of free and bound phenolics and antioxidant activity when compared to pulp and seeds, and showed antimicrobial activity against Bacillus cereus and Escherichia coli. The atemoya seed oil had α-tocopherol (19 mg kg−1 oil) and γ-tocopherol (90 mg kg−1 oil), and significant amounts of unsaturated fatty acids (~80%): 43% monounsaturated and 36% polyunsaturated. The study showed that atemoya and its by-products have potential applications in the food, cosmetic and pharmaceutical industries.

Physicochemical, microbiological and sensory characterization of liquor obtained from atemoya fruit (Annona cherimola×Annona squamosa)

En este estudio se realizó la caracterización fisicoquímica, microbiológica y sensorial del licor obtenido a partir del fruto de atemoya. La bebida fermentada fue elaborada empleando pulpa de fruta (5 %), agua (78 %), acidulante (ácido cítrico 0.04 %), regulador de pH (fosfato diamónico 0.04 %), azúcar (15 %) y levadura (Saccharomyces cerevisiae 1.92 %). El proceso de obtención del licor incluyó una etapa de selección, limpieza y desinfección del fruto, caracterización de la pulpa, despulpado, mezclado, fermentación, filtración, clarificación y envasado. Los resultados obtenidos evidenciaron una buena aceptación sensorial en relación a la textura, color, sabor y olor. Se obtuvo en el producto final un pH de 3.78, acidez volátil de 0.08 %, densidad de 1.10 g/mL, azúcares reductores de 10.56 %, grado de alcohol de 9.77 °GL, porcentaje de metanol de 67 ppm, E. coli ausente, recuento de mesófilos aerobios de 90 UFC/mL y recuento de hongos y levaduras de 50 UFC/mL. Dado lo anterior, se concluye que el licor obtenido es una bebida estable desde el punto de vista fisicoquímico, microbiológico y sensorial, debido a que cumple con los requisitos de calidad exigidos por la Norma Técnica Colombiana 708 de 2000, establecida para vinos de frutas.

Vegetal regulators increase the quality of atemoya fruits and recover the photosynthetic metabolism of stressed plants

Vegetal regulators such as auxin, cytokinin, and gibberellin were applied in different concentrations, through commercial mix, to “Thompson” variety of atemoya fruits to verify the effect of the regulators in the quality of healthy plants of atemoya fruit and its influence in the gas exchange of the stressed plants. The study was conducted with two experiments: the first experiment consisted in the application of the mix of auxin, cytokinin, and gibberellin with concentrations of 0, 200, 300, 400, and 600 mL ha−1, during 14 days, for 5 months, in commercial orchard and in healthy plants. There were evaluated length, diameter, fresh mass of the peel, fresh mass of the pulp, and total fresh mass; fruits’ volume, soluble solids and pH of the pulp, and number and fresh mass of the seeds. In the second experiment, the application of the regulators’ mix in the concentrations that resulted in better quality of the fruits such as 300 and 400 mL ha−1 was conducted in stressed plants to evaluate the recovery of the photosynthetic metabolism and of the physical–chemical quality of the fruits. In this experiment, the control treatment (without application of the mix) was conducted in the plants with good visual conditions and highest numbers of greener leaves, smaller leaves and yellowish leaves. The gas exchanges were conducted every 14 days, and when the fruits ripened, they were harvested for the measurement of ascorbic acid, soluble sugars, reducing sugars, sucrose, and starch. The concentrations of the mix of auxin, cytokinin, and gibberellin such as 300 and 400 mL ha−1 improved the quality of the fruits and recovered the gas exchange of stressed plants without modifying the physical–chemical characteristics of its fruits, setting itself as an agricultural practice that is beneficial for the atemoya fruit cultivation.

Transcriptome analysis of atemoya pericarp elucidates the role of polysaccharide metabolism in fruit ripening and cracking after harvest

BackgroundMature fruit cracking during the normal season in African Pride (AP) atemoya is a major problem in postharvest storage. Our current understanding of the molecular mechanism underlying fruit cracking is limited. The aim of this study was to unravel the role starch degradation and cell wall polysaccharide metabolism in fruit ripening and cracking after harvest through transcriptome analysis.ResultsTranscriptome analysis of AP atemoya pericarp from cracking fruits of ethylene treatments and controls was performed. KEGG pathway analysis revealed that the starch and sucrose metabolism pathway was significantly enriched, and approximately 39 DEGs could be functionally annotated, which included starch, cellulose, pectin, and other sugar metabolism-related genes. Starch, protopectin, and soluble pectin contents among the different cracking stages after ethylene treatment and the controls were monitored. The results revealed that ethylene accelerated starch degradation, inhibited protopectin synthesis, and enhanced the soluble pectin content, compared to the control, which coincides with the phenotype of ethylene-induced fruit cracking. Key genes implicated in the starch, pectin, and cellulose degradation were further investigated using RT-qPCR analysis. The results revealed that alpha-amylase 1 (AMY1), alpha-amylase 3 (AMY3), beta-amylase 1 (BAM1), beta-amylase 3 (BAM3), beta-amylase 9 (BAM9), pullulanase (PUL), and glycogen debranching enzyme (glgX), were the major genes involved in starch degradation. AMY1, BAM3, BAM9, PUL, and glgX all were upregulated and had higher expression levels with ethylene treatment compared to the controls, suggesting that ethylene treatment may be responsible for accelerating starch degradation. The expression profile of alpha-1,4-galacturonosyltransferase (GAUT) and granule-bound starch synthase (GBSS) coincided with protopectin content changes and could involve protopectin synthesis. Pectinesterase (PE), polygalacturonase (PG), and pectate lyase (PEL) all involved in pectin degradation; PE was significantly upregulated by ethylene and was the key enzyme implicated pectin degradation.ConclusionBoth KEGG pathway enrichment analysis of DEGs and material content analysis confirmed that starch decomposition into soluble sugars and cell wall polysaccharides metabolism are closely related to the ripening and cracking of AP atemoya. A link between gene up- or downregulation during different cracking stages of atemoya fruits and how their expression affects starch and pectin contents were established by RT-qPCR analysis.

Atemoya fruit development and cytological aspects of GA3-induced growth and parthenocarpy.

The exogenous application of GA3 to atemoya tree flowers induces parthenocarpy, and in association with artificial pollination, it increases the fruit size. Morphological, anatomical, ultrastructural, and chemical aspects were evaluated during development of (1) fruit produced by artificial pollination (AP), (2) fruit from AP followed by the application of 250ppm GA3, and (3) parthenocarpic fruit induced by the application of 1000ppm GA3. Fruit growth showed a sigmoidal pattern, with development occurring in three phases: (I) cell division, (II) cell differentiation, and (III) maturation. Phase I presented cells with large nuclear volumes and a large population of organelles, phase II presented cells with a reduction in cytoplasm and an increase in vacuole volume, and phase III presented cells with an increase in plastids with reserve compounds. The application of GA3, in association with pollination, precedes cytological events and delays when applied exclusively. GA3 promotes the growth of pollinated fruits by stimulating cell division and expansion, which occur in association with reduced seed production, and the GA3 induces parthenocarpy by maintaining division and stimulating cell expansion. The absence of seeds accounts for the smaller size of the parthenocarpic fruits, and the lower accumulation of calcium accounts for less firm fruit.

Modified Atmosphere and Refrigeration in Postharvest Conservation of Atemoya cv. Gefner

Aims: The objective of this study was to evaluate the effect of the modified atmosphere under refrigeration for 28 days in the Atemoya Gefner.&#x0D; Study Design: Experiment 1 and 2 was installed in a completely randomized design, in a factorial 1x4 and 2x8, respectively.&#x0D; Place and Duration of Study: The experiment was conducted in a cold chamber at the State University of Montes Claros, Brazil, between July and August 2018.&#x0D; Methodology: In the first, the percentage of fruits that presented cold injury (CI) in unpackaged fruits and stored at 15°C for six days was visually evaluated. In the second experiment two pack were used and stored at 15°C and evaluated the physical and chemical characteristics.&#x0D; Results: In the first experiment, from the 2nd day 100% of the fruits showed CI that progressed to stage 3 on the 6th day of storage. This result justifies the need to store the atemoya under modified atmosphere under 15°C for 28 days. It was found at the end of 28 days, experiment two, that the packages 1 and 2 showed quality fruits, with characteristics of ripe fruits, with 22 °Brix average values, reduced titrable acidity, light green color peel and good lightness. The pack1 showed better weight maintenance, with 1.54% of fresh weight loss against 2.36% for pack2.&#x0D; Conclusion: There was a higher solubilization of total sugars and non-reducing sugars in pack1, being this the most indicated for atemoya fruits grown in northern Minas Gerais.

Comparative transcriptomic analysis of split and non-split atemoya (Annona cherimola Mill. × Annona squamosa L.) fruit to identify potential genes involved in the fruit splitting process

Atemoya (Annona cherimola Mill. × Annona squamosa L.), a popular tree with high medicinal and nutritional properties in fruit, is widely cultivated in tropical South Asia, East Asia and America. As a typical climacteric fruit, atemoya fruit has a short shelf life, and splitting frequently occurs during the postharvest period. However, little information is available on the mechanism underlying fruit splitting. To gain a comprehensive perspective on the splitting process of atemoya fruit, various cDNA libraries were prepared independently for Illumina sequencing. In total, 79,699,184 reads were assembled into 38,518 unigenes. A total of 17,860 genes, including 16,190 up- and 1670 down-regulated genes, were identified as differentially expressed genes (DEGs). A number of DEGs have been assigned to most of the key components involved in different hormonal signaling pathways, indicating that the splitting process of atemoya fruit may be controlled by an intricate hormonal signaling network. Furthermore, the expression levels of most PG-, PME-, and cellulase-encoding genes increased in the split fruits compared with the non-split fruits, which likely contributed to the reduction in skin elasticity. Identification of the DEGs related to phytohormones, and cell wall will facilitate the understanding of the regulatory mechanism underlying the fruit splitting process of atemoya.

Gibberellic acid combined with hand pollination increases ‘Red’ and ‘Lessard Thai’ sugar apple fruit quality and produced parthenocarpic ‘Gefner’ atemoya fruits

ABSTRACT: The present research aimed to evaluate the effect of applying gibberellic acid (GA3) with hand (HP) or natural pollination (NP) on fruit set and the fruit quality of ‘Red’ and ‘Lessard Thai’ sugar apple and ‘Gefner’ atemoya fruits.This study was performed in an experimental orchard located in Homestead, Florida, USA. The experimental design included randomized blocks, with eight treatments, four replicates and four plants per plot. Treatments included: (1) HP; (2) HP + 10 mg L-1 GA3; (3) HP + 100 mg L-1 GA3; (4) HP + 1,000 mg L-1 GA3; (5) NP; (6) NP + 10 mg L-1 GA3; (7) NP + 100 mg L-1 GA3 and (8) NP + 1,000 mg L-1 GA3. The HP plus 1,000 mg L-1 GA3 promoted fruit setting above 90% over the 14 weeks for all genotypes evaluated. Significant increments for length and total fruit weight were observed. ‘Red’ sugar apple and atemoya had a reduced number of seeds per fruit. The NP plus GA3 (1,000 mg L-1) was effective in producing high quality seedless ‘Gefner’ atemoya fruits. This investigationdemonstratedthat GA3 plus hand pollination produced high quality sugar apple and atemoya seeded fruits and in association with natural pollination promoted seedless ‘Gefner’atemoya fruits.

In Vitro Antioxidant Activity of Aqueous Extracts from the Atemoya Fruit (Peel, Pulp, and Seed): Correlation of Their Protein, Carbohydrate, and Phenolic Compound Contents

The properties of several fruits still remain unevaluated, with regard to being the source of antioxidant compounds, one of such fruits being atemoya. In this study, three parts (seed, pulp, and peel) of atemoya were submitted to extraction.

Colletotrichum theobromicolaによるアテモヤ炭疽病 (新称) の発生

Colletotrichum theobromicolaによるアテモヤ炭疽病 (新称) の発生

Gonodonta SPECIES (Lepidoptera: Erebidae) DEFOLIATING SUGAR APPLE AND ATEMOYA FRUITS IN THE SEMIARID REGION OF MINAS GERAIS

ABSTRACT This paper aimed to evaluate the occurrence of Gonodonta larvae defoliating sugar apple (Annona squamosa) and atemoya fruits (Annona squamosa x Annona cherimolia L. Mill.) in northern state of Minas Gerais. Larvae were collected between December 2007 and January 2008 in Annonaceae plantation in the rural area of the municipality of Janaúba, MG. Adults obtained have been sent for identification. The species identified were Gonodonta bidens Geyer, G. nutrix Stoll and G. pyrgo Cramer. The infestation level observed in the orchard was 85% of plants with the presence of Gonodonta larvae and the average defoliation percentage was 30%. These are the first records of Gonodonta species defoliating sugar apple and atemoya in Brazil.

Mineral composition, nutritional properties, total phenolics and flavonoids compounds of the atemoya fruit (Annona squamosa L. x Annona cherimola Mill.) and evaluation using multivariate analysis techniques.

The atemoya is a hybrid fruit obtained by crossing of cherimoya (Annona cherimola Mill.) with sweet sop (Annona squamosa L.). The information about chemical composition of atemoya is scarce. The mineral composition was evaluated employing Inductively Coupled Plasma Optical Emission Spectrometry (ICP OES) and the centesimal composition and the physico-chemical parameters were assessed employing procedures described in the AOAC methods. The total phenolic compounds (TPC) and total flavonoids (TF) were determined using spectroanalytical methods. Considering the Reference Daily Intake (RDI), the concentrations of K, Cu and Vitamin C found in atemoya were the highest, representing about 32, 23 and 37% of the RDI, respectively. The total carbohydrates were 32 g 100g-1 and the soluble solids was equivalent to (32.50 ± 0.03) °Brix. The result for TPC was 540.47 ± 2.32 mgGAE 100 g-1 and the TF was 11.56 ± 1.36 mgQE 100 g-1. The exploratory evaluation of 42 atemoya samples was performed through Principal Component Analysis (PCA), which discriminated green and ripe fruits according to their mineral composition. The elements that contributed most for the variability between green and ripe fruits were: Ba, Ca, Cu, K, Mg and P.

Uso do 1-MCP e atmosfera modificada na pós-colheita de atemoia ‘Gefner’

The scarcity of technologies related to the post-harvest physiology of this species is one of the barriers to commercialization of atemoya over long distances, aggravated by perishability presented by them. Thus, the objective of this study was to prolong the shelf life of atemoya fruits using 1-methylcyclopropene (1-MCP) and modified atmosphere by low-density polyethylene (LDP), associated with refrigeration. The experiment was conducted in a completely randomized design in a split-plot, with plots in the four doses of 1-MCP (0, 200, 400 and 600 ?L L-1), and in the 5 subplots evaluation periods after harvest in the range of 5 days, with four replicate. Atemoyas were harvested at physiological maturity then treated with doses of 1-MCP for 8 h at room temperature. Soon after, the fruits were placed in polystyrene trays. Some remained unpacked and others were packed with a plastic film of LDP 16 ?m. The use of modified atmosphere and 1-MCP alone or combined, were effective in delaying the ripening of fruits, allowing the preservation of its chemistry quality. In the fruits treated with 1-MCP was no delay in the accumulation of soluble solids and starch degradation.

Identificação de espécies de Colletotrichum associados à antracnose em plantas de atemóia e colonização do fungo nos frutos

A atemóia é um híbrido Annona cherimola com A. squamosa. A antracnose, causada por Colletotrichum sp., é uma importante doença da atemóia, causando danos em diferentes órgãos da planta, destacando àqueles causados nos frutos, tanto na pré como na pós-colheita. Diante deste problema, o presente trabalho teve como objetivo realizar a identificação de espécies de Colletotrichum associados à antracnose em plantas de atemóia através do seqüenciamento de diferentes regiões do DNA deste fungo e acompanhar as etapas de colonização de frutos de atemóia por este fungo através de microscopia eletrônica de varredura. Após extração de DNA, foi realizado o seqüenciamento dos genes da β-tubulina e α-elongase e da região do ITS-5.8S rDNA do DNA dos fungos. Das 15 amostras sequenciadas seis foram identificadas como Colletotrichum acutatum e as outras foram identificadas como C. boninense. A espécie C. acutatum foi encontrada somente em amostras obtidas de folhas de atemóia, enquanto que a espécies C. boninense foi identificada de amostras obtidas de frutos, ramos e folhas doentes. Todas as etapas da doença ocorreram nas 48 horas, sendo que foi observada a germinação dos esporos entre duas e quatro horas após a inoculação

Preservação dos atributos físicos de frutos de atemoia cv. Gefner com o uso de 1-MCP e atmosfera modificad

O interesse comercial pela atemoia vem aumentando cada vez mais no Brasil. O aumento da atividade respiratória, acompanhado por modificações rápidas na composição física dos frutos podem inviabilizar sua distribuição a mercados distantes. Neste contexto, objetivou-se com o presente trabalho avaliar os atributos físicos em frutos de atemoia tratados com 1-metilciclopropeno (1-MCP) e atmosfera modificada associados à refrigeração. O experimento foi instalado em delineamento experimental inteiramente casualizado, em esquema fatorial 4x5, sendo quatro concentrações de 1-MCP (0; 200; 400 e 600 ŋL L-¹) e cinco períodos de avaliação após a colheita, em intervalo de cinco dias, com quatro repetições e quatro frutos por unidade experimental. As atemoias foram colhidas em pomar comercial, no município de Matias Cardoso-MG, no estádio de maturação fisiológica, lavadas, sanitizadas e secas ao ar, depois tratadas com as concentrações de 1-MCP por oito horas, em temperatura ambiente. Logo após, quatro frutos foram dispostos em bandejas de poliestireno expandido. Alguns permaneceram sem membrana, enquanto outros foram embalados com membrana plástica de PEBD 16µm. As variáveis avaliadas nos frutos foram: perda de biomassa fresca, firmeza, concentração de CO2, etileno e coloração da casca. O uso da atmosfera modificada e do 1-MCP, associados ou não, foi eficiente no atraso do amadurecimento dos frutos, permitindo a conservação de sua qualidade física. Frutos tratados com 1-MCP apresentaram-se mais firmes, além da preservação da coloração, verificada através da luminosidade, cromaticidade e ângulo Hueº, que se mostraram superiores quando comparados aos frutos não tratados.