Association Of Official Analytical Chemists Research Articles

Sorption Isotherm and Effect of Packaging Materials on Proximate, Organoleptic and Lipid Oxidation Quality of Chicken Nuggets Stored at Two Different Temperatures

The study Investigated sorption isotherm features and the effects of packaging materials of both high-density and low-density polyethylene on sensory properties, proximate composition and lipid oxidation of chicken nuggets in storage. Boneless chicken breast was procured from a reputable source and cut into sizeable weight of 50g each to produce the chicken nuggets used for the studies. The experimental setup for sorption isotherm analysis in this study involved using a gravimetric method to equilibrate the product at various relative humidity levels. Five (5g) grams of oven-dried chicken nugget samples of known moisture content were placed in a controlled humidity chamber, and the weight changes of the samples were monitored until equilibrium was reached. GAB equation was used to evaluate the product ware activity and monolayer moisture. Sensory assessment was evaluated using a 9- point hedonic scale while proximate composition of the chicken nuggets samples was determined according to the methods of the Association of Official Analytical Chemists. Thiobarbituric acid reactive substances (TBARS) assay was used to measure malondialdehyde (MDA) levels as a marker of lipid oxidation. The investigation showed that sensory qualities, proximate composition and lipid oxidation of chicken nuggets were markedly affected by the variance in temperature settings, with distinct disparities seen among different materials for packaging. Chicken nuggets stored at 25 0C caused a significant loss in organoleptic properties and some nutritional composition such as protein and fat. Storage of chicken nugget at 25 0C and 40 C showed a varied values in malondialdehyde as content ranged from 0.52 to 0.81 for chicken nugget stored at 250 C and 0.17 to 0.43 for the product stored at 40 C across the packaging materials investigated. The water activity and monolayer moisture results of sorption analysis revealed that good packaging materials of very low water vapour transmission rate should be used for storage of chicken nuggets as high-density polyethylene offered a promising alternative than low density polyethylene.

AN ANALYSIS OF MICRO-NUTRIENTS COMPOSITION OF COCOYAM (COLOCASIAE ESCULENTA) AND SWEET POTATO (IPOMOEA BATATAS L) LEAVES IN MELON SOUP

The study ascertained the micro-nutrient composition of cocoyam and sweet potato leaves in melon soups. The study was conducted to identify vitamins and mineral composition in three types of melon soups. The instrument adopted for the study was laboratory analysis of food nutrients using official methods of Association of Official Analytical Chemists. The data for micro nutrients were collected in triplicates from the melon soups in the food analysis laboratory, raw triplicate values of each micro nutrient in the soups were collected and analyzed using ±SD and ANOVA statistics to test the hypotheses 1 – 4. The findings revealed that cocoyam and sweet potato leaves melon soups are as rich as fluted pumpkin leaf melon soup in micro nutrients, such as vitamin A (Iµ) 16.03: 16.80 while fluted pumpkin 19.88; vitamin C (mg) 26.30: 36.89 while fluted pumpkin 28.46; folic acid (mg) 33.44: 40.64 while fluted pumpkin 46.73 among others. For minerals- Na (mg) 29.80: 26.16 while fluted pumpkin 34.19; Fe (mg) 1.41: 1.60 while fluted pumpkin 1.24 K (mg) 36.08: 46.46 while fluted pumpkin 34.57; Ca (mg) 11.19: 8.10 while fluted pumpkin 5.88; Mg (mg) 4.62: 3.53 while fluted pumpkin 2.63 among others. The study also showed that there was a significant difference between the mean values of the micro-nutrients analysis. The study recommended that, the consumption of cocoyam and sweet potato leaves should be encouraged by families in the country, as well be made available in the open market same way the popular vegetables are sold such as fluted pumpkin (Ugu) leaf.

CORRELATION ANALYSIS AND MODELLING OF HEAVY METAL CONCENTRATIONS AND PHYSICOCHEMICAL PARAMETERS FROM SOIL SAMPLES OF FIVE SELECTED REFUSE DUMPSITES IN LAFIA LOCAL GOVERNMENT AREA OF NASARAWA STATE, NIGERIA

Thousands of tons of solid wastes are generated and disposed daily in open dumps of most Nigerian cities due to rapid population explosion and commercial activities. In this study, concentrations of heavy metals and physicochemical parameters from 20 soil samples each from selected refuse dumpsites in wet and dry seasons were determined using Atomic Absorption Spectrophotometer and Association of Official Analytical Chemists (AOAC) standard methods. The results showed that during the wet and dry seasons, the concentration of heavy metals, Hg, Cr, Mn were above that of World Health Organisation (WHO), and National Environmental Standards and Regulations Enforcement Agency (NESREA), while that of Pb, As, Fe, Zn, Cu and Ni were below the tolerable limits. The results for physicochemical parameters in wet and dry seasons for nitrates, phosphates and organic matter were above WHO standards. Statistical test at 0.05 probability level (p < 0.05) was used to determine the degree of association between pairs of the variables. The principal component analysis (PCA) was performed to measure the direct and inverse relationship between these parameters and heavy metal availability. Based on the different variables obtained at the study areas, a multiple linear regression analysis was determined from which a general linear model was formulated. The study recommends appropriate government agencies should as a matter urgency integrate the regular monitoring of waste disposal into the state developmental plan and framework in order to prevent excessive build-up of these metals in humans through the food chain.

Quality assessment of smoked catfishes (Clarias gariepinus, Heterobranchus longifilis and Synodontis clarias) from selected fish markets in Benue State, Nigeria

The quality assessment of smoked catfish from selected fish markets in Benue state was carried out. Clarias gariepinus, Heterobranchus spp. and Synodontis spp. each weighing 500g were collected from Abinsi and Wadata markets. The proximate composition, mineral composition and organoleptic assessment were carried out. Association of Official Analytical Chemists (AOAC) methods were adopted for the determination of proximate and mineral composition while a 9-point Hedonic scale ranging from 1(dislike extremely) to 9(like extremely) was a procedure for organoleptic quality determination. Results for proximate and mineral compositions showed good standard but varied from market to market. Abinsi fish markets showed that Heterobranchus spp. had 10.16%, 12.74%, 58.98%, 16.42%, 1.71%. Clarias gariepinus had 9.09%, 10.10%, 66.11%, 13.94%, 0.76%. Synodontis spp. had 8.19%, 11.07%, 55.80%, 22.68%, and 2.27% as values for moisture content, ash content, protein content, lipid content and carbohydrates content while Wadata fish market Heterobranchus spp. had 10.34%, 11.89%, 59.48%, 15.71%, and 2.59%. Clarias gariepinus had 8.88%, 11.37%, 68.97%, 10.37%, 0.38%. Synodontis spp. had 8.36%, 12.19%, 56.35%, 21.30%, and 1.81% for moisture content, ash content, protein content, lipid content and carbohydrates content respectively. The mineral composition for Abinsi fish showed that Heterobranchus spp. had 240.33 mg/100g, 395.48 mg/100g, 32.44 mg/100g, 0.03 mg/100g, 0.80 mg/100g, 5.37 mg/100g; Clarias gariepinus had 270.30 mg/100g, 278.05 mg/100g, 32.14 mg/100g, 0.21 mg/100g, 0.30 mg/100g, 5.06 mg/100g; Synodontis spp. had 330.05 mg/100g, 257.78 mg/100g, 34.24 mg/100g, 0.24 mg/100g, 0.40 mg/100g, 6.98 mg/100g; for Potassium, Calcium, Sodium, Copper, Zinc and Iron while in Wadata fish market Heterobranchus spp. had 250.30 mg/100g, 127.88 mg/100g, 39.84 mg/100g, 0.39 mg/100g, 0.68 mg/100g, 4.95 mg/100g; Clarias gariepinus had 410.15 mg/100g, 159.33 mg/100g, 33.03 mg/100g, 0.22 mg/100g, 0.60 mg/100g, 5.96 mg/100g; Synodontis spp. had 300.45 mg/100g, 295.48 mg/100g, 33.93 mg/100g, 0.30 mg/100g, 0.71 mg/100g, 12.0 mg/100g; for Potassium, Calcium, Sodium, Copper, Zinc and Iron. The sensory evaluation revealed that taste, appearance, texture and odour were accepted by the panellist and significant difference occurred (P<0.05) among samples from both markets. The three catfish species from this study were found to be rich in protein content, Potassium, Calcium, and Sodium with low presence of Copper, Zinc, Iron, lipid and carbohydrate contents.Keywords: Catfishes; markets; quality assessment; smoked

Variation in nutritional composition of Strychnos spinosa Lam. morphotypes in KwaZulu-Natal, South Africa

AbstractFruits of Strychnos spinosa Lam. hold considerable food value within rural communities. However, no study has reported the nutritional profile of S. spinosa morphotypes. Therefore, this study is aimed to determine nutritional variation among the morphotypes. Proximate composition was analyzed using association of official analytical chemists’ methods, and minerals with inductively coupled plasma atomic emission spectroscopy. The following were the ranges of proximate and nutrient content determined: moisture content (10.29–60.50%); fat (0.95–2.67%); crude protein (2.85–9.19%); ash (4.78–18.05%); carbohydrates (37.39–42.24%); acid detergent fibre (7.94–21.75%), neutral detergent fibre (16.46–42.55%); calcium (0.30–35 mg/100 g); potassium (810–2510 mg/100 g); phosphorus (9–69 mg/100 g); sodium (7–54 mg/100 g); magnesium (9–55 mg/100 g); copper (0.10–2.70 mg/100 g); iron (0.10–5.50 mg/100 g); manganese (0.30–2.43 mg/100 g) and zinc (0.10–0.80 mg/100 g). Calcium, phosphorus, magnesium, copper, iron, manganese, fat, acid detergent fibre, neutral detergent fibre, sodium, crude protein showed positive association with principal components. Biplot and dendrogram grouped morphotypes with high and low nutrient content independently. Carbohydrates, protein, calcium, potassium, copper, iron, manganese, and zinc content of morphotypes were higher than those in commercialized fruits. This species is promising for domestication and commercialization, and thereby contributes significantly towards food security.

Screening of 152 Veterinary Drug Residues in Animal Source Foods by LC–MS/MS, Multilaboratory Validation Study: Final Action 2020.04

Abstract Background The presence of veterinary drug residues in food-producing animals and animal products is regulated through the enforcement of maximum residue limits (MRLs). To answer the need of the food sector to monitor these substances in a wide range of food commodities, stakeholders at AOAC INTERNATIONAL identified the need for a reliable confirmatory screening method. Such a qualitative approach is required for compliance checking and to support product release in manufacturing. Objective Data were collected from five independent laboratories that applied the First Action Official Method 2020.04 to demonstrate adequate performance under reproducibility conditions. The probability of detection (POD) was calculated in blank test samples and test samples spiked at the screening target concentration (STC) level, with the objective to achieve PODs ≤10% and ≥90%, respectively. Additionally, the effectiveness of the screening method was evaluated by participating in 92 proficiency tests. Methods Four streams were optimized to screen for 152 veterinary drug residues by LC–MS/MS in a wide variety of food commodities including milk-based ingredients and related products (e.g., milk fractions, infant formula, infant cereals, and baby foods), meat- and fish-based ingredients and related products (fresh, powdered, cooked, infant cereals, and baby foods), and other ingredients based on eggs, animal fat, and animal byproducts. The four streams covered 105 antibiotic residues, anti-inflammatory and antiparasitic agents (stream A), 23 beta-lactams (stream B), 14 aminoglycosides (stream C), and 10 tetracyclines (Stream D). Results The multilaboratory validation led to PODs at the STC ≥94% and PODs in the blank ≤9%. Further application of the multilaboratory validated method to 92 proficiency tests provided more than 99% satisfactory submitted results (n = 784). Conclusion The interlaboratory reproducibility determined for this method met the acceptance criteria defined in AOAC Standard Method Performance Requirement (SMPR) 2018.010. Highlights AOAC has approved the method for Final Action status.

Profile of stingless bee honey and microbiota produced in West Sumatra, Indonesia, by several species (Apidae, Meliponinae)

Background and Aim: Stingless bees are generally found in tropical countries, including Indonesia. In West Sumatra, stingless bees are known as Galo-galo, consist of several species with different characteristics; however, the properties of honey produced by stingless bees have not yet been explored. This study aimed to determine the physicochemical, antioxidant, and antimicrobial activities as well as the microbiota profile of stingless bee honey from the bee species Heterotrigona itama, Geniotrigona thoracica, Tetrigona melanoleuca, and Tetrigona binghami that are intensively developed in West Sumatra, Indonesia. Materials and Methods: Honey produced by the stingless bee species H. itama, G. thoracica, T. melanoleuca, and T. binghami originating in West Sumatra was examined in the present study. The physicochemical properties (Association of Official Analytical Chemists), antioxidant activity (2,2-diphenyl-1-picrylhydrazyl technique), total phenols (Folin- Ciocalteu method), antimicrobial activity (Agar-Well diffusion test), total lactic acid bacteria, and microbiota diversity were measured in stingless bee honey samples. Results: Stingless bee species significantly affected the physicochemical properties, antioxidant activity, total phenolic content, antimicrobial activity, and total lactic acid bacteria (p = 0.05), except for the crude fiber content. The carbohydrate profiles of honey produced by H. itama and T. binghami were dominated by monosaccharides, whereas those of honey from T. melanoleuca and G. thoracica were dominated by disaccharides. In terms of antioxidant activity (half maximal inhibitory concentration [IC50] value), there were no significant differences (p > 0.05) between honey from H. itama, T. melanoleuca, and T. binghami, but there were significant differences (p > 0.05) between honey from G. thoracica. The honey of G. thoracica and T. melanoleuca had the highest total phenolic content (65.65 ± 14.00 and 69.78 ± 8.06, respectively). In addition, honey from the four stingless bee species showed antimicrobial activity against the pathogenic bacteria Escherichia coli, Salmonella, Staphylococcus aureus, and Listeria monocytogenes. From the principal co-ordinate analysis (PCoA) results, it can be concluded that the microbiota profiles of the four stingless bee honey samples differed. Conclusion: The results showed that honey from H. itama, G. thoracica, T. melanoleuca, and T. binghami has different physicochemical characteristics, antioxidant activity, antimicrobial activity, and microbiota diversity. By knowing the content of this stingless bee honey, the results of this study can be used as information that this stingless bee honey has the potential as a functional food that is beneficial for health. Keywords: antimicrobial, antioxidant activity, microbiota, physicochemical, stingless bee.

Phytochemical Evaluation and Lethal Concentration of Aqueous Extract of Adansonia digitata Fruit Pulp in Clarias gariepinus

The study was aimed at assessing the lethal concentration 50 (LC50) of aqueous extract of Adansonia digitata fruit pulp (AEADFP) in Clarias gariepinus. Adansonia digitata fruit pulp was obtained at the Faculty of Veterinary Medicine, Ahmadu Bello University (A.B.U), Zaria, Nigeria. Proximate nutrient composition was determined according to the Association of Official Analytical Chemist. Maceration method was employed for Adansonia digitata fruit pulp extraction using distilled water, and thereafter, screened for phytochemical constituents. A total of 200 African catfish (Clarias gariepinus) weighing between 185 ± 5 g (27-36 cm) were used for determination of LC50 of AEADFP using standard procedure at the aquaculture animal house Unite of Department of Veterinary Pathology, A.B.U., Zaria. Twenty (20) fish were used for range finding test, while 180 fish for LC50 evaluation. The experimental fish were observed for clinical signs of toxicity and mortality. The result of proximate nutrient composition of Adansonia digitata fruit revealed the presence of carbohydrate, lipid, protein, ash, moisture, and crude fiber. Qualitative phytochemical evaluation revealed the presence of tannins, flavonoids, phenol, terpenoid, glycosides, saponins, anthraquinone, steroids, reducing sugar and alkaloids, while quantitative phytochemical assay showed that total Phenol and alkaloid had highest and lowest concentration, respectively. There was neither mortality nor any clinical sign of toxicity observed for the 96 hours post-administration to AEADFP up to 5000 mg/L. Conclusively, AEADFP had nutritional and bioactive compounds that are important in biological processes. The lethal concentration of AEADFP is above 5000 mg/L; hence, relatively safer for use in medicinal purposes.

Nutritional Composition of Some Selected Commercial Juice Made in Nigeria

Introduction: Nutrient-fortified food play a crucial role in addressing nutritional deficiencies among infants and children, making them more appealing for consumption. Research focuses on baby foods, particularly reconstituted juice drink, which serve as significant sources of essential minerals and vitamins. However, the nutrient composition of these products varies, leading to differences in quality. The objective of this study is to assess the disparities in the nutritional content of various children's juice drink available in the Nigerian market. Methods: Proximate analysis was carried out according to the standard procedure of Association of Official Analytical Chemists (AOAC) to evaluate the nutritional composition of the selected juice drink. Minerals content were determined using Atomic Absorption and Flame Spectroscopy Techniques. The determination of the Vitamin A content in the samples was conducted following a modified Spectrophotometric Method. For Ascorbic Acid (Vitamin C), the Vitamin C content in the samples was assessed utilizing a Spectrometric Method coupled with 2,4 Dinitrophenyl hydrazine (DNPH) procedures. Results: Examination of the proximate and mineral analyses of ten chosen fruit juice brands revealed notable variations. Capri sonne exhibited the highest moisture content at 94.2%, while Hollandia Yogurt has the lowest at 81.3%. Chapman, Nutri Milk, and Viju Apple Flavor Milk shared the highest ash content at 0.5%, whereas Hollandia Malt, Piko Orange, and Piko Strawberry displayed the lowest at 0.01%. Nestle Malt boasted the highest crude protein concentration at 4.38%, with Capri sonne recorded the lowest at 0.18%. Hollandia Yogurt and Hollandia Malt demonstrated the highest crude fiber content, while Vitavite Multivi, Piko Orange, and Piko Strawberry showed the lowest. Regarding total carbohydrates, Hollandia Yogurt topped the list at 14.6%, while Vitavite Multivi ranked lowest at 6.57%. Nestle Millo contained the highest vitamin C content at 25.7%, whereas Piko Strawberry has the lowest at 3.82%. Caprosonne registered the highest vitamin A content at 0.6%. In terms of mineral analysis, Hollandia Yogurt exhibited the highest sodium percentage at 22.6mg/g, while Nestle led in calcium, iron, and magnesium content at 120.4mg/g, 1.7mg/g, and 25.5mg/g, respectively. It can therefore be inferred from the outcome of this study that, Milo Nestle is the best drink for children due to its higher protein, carbohydrate, vitamins (A and C) and minerals (Ca, Mg, Fe, Na) contents, followed by Hollandia Malt as compared to other juice studied.

Nutritional Composition and Antinutritional Factors of Five Wild Edible Fruits Grown in the Mekdela District, South of Wollo, Ethiopia.

This study was carried out to determine the mineral content and nutritional properties of five wild fruits Rhus vulgaris, Rosa abyssinica, Rhus natalensis, Euclea racemosa, and Ficus sur. The proximate composition parameters (moisture, ash, crude fiber, crude fat, and crude protein) and antinutritional factors were evaluated using methods of the Association of Official Analytical Chemists and elemental analysis using the atomic absorption spectroscopy technique. Among the five wild edible fruit species, Rhus vulgaris had the highest carbohydrate content (83.3 ± 0.28 g/100 g) and a high total energy (344.5 ± 2.21 kcal/100 g). Euclea racemosa was found to have the maximum ash content (12.8 ± 0.37 g/100 g), protein content (3.22 ± 0.01 g/100 g), and moisture (16.24 ± 0.003 g/100 g), respectively. Rhus natalensis showed the highest fiber content (9.54 ± 0.003 g/100 g). Mineral analysis showed that local wild fruits contained a considerable amount of minerals. The calcium concentration ranged from 99.51 mg/100 g in Euclea racemosa to 160.12 mg/100 g in Ficus sur. Potassium concentration varied from 54.34 mg/100 g in Euclea racemosa to 234 mg/100 g in Rhus vulgaris. Iron ranges from 21.4 mg/100 g in Rosa abyssinica to 41 mg/100 g in Rhus natalensis, and zinc ranges from 2.3 mg/100 g in Rhus vulgaris to 4.2 mg/100 g in Ficus sur. A high saponin content (2.12 mg/100 g) and a low tannin content (0.23 mg/100 g) were obtained in Rosa abyssinica. The phytate content (1.52 mg/100 g) and the oxalate content (0.9 mg/100 g) were high in Rhus natalensis. In conclusion, the present study shows that wild fruits can be used as food supplementation in food in a safe area.

Simultaneous determination of four non paraben preservatives (benzyl alcohol, phenoxyethanol, sodium benzoate, and salicylic acid) in shampoos by an isocratic HPLC method

Salicylic acid (SA), sodium benzoate (SB), phenoxyethanol (PO), and benzyl alcohol (BA) are “non paraben” preservatives commonly used in cosmetic products like shampoos, and often in combination of at least two compounds to assure their antimicrobial effectiveness at low concentrations. In this study, an HPLC-DAD method was developed and validated for simultaneous determination of SA, SB, PO, and BA in commercial shampoos. The method was carried out using an YMC Triart C18 (250 × 4.6 mm, 5 µm) column with a mixture of methanol and 0.02 M solution of phosphate buffer pH 2.7 (35 : 65, v/v) as mobile phase. The flow rate of mobile phase was maintained at 1.0 mL per minute, the injection volume was 20 µL and the UV detection was done at 208 nm for BA and at 230 nm for SA, SB and PO. The method was fully validated according to requirements on analytical method performance of AOAC International and validation results confirmed its reliability and suitability for intended application. The method was employed to determine the four “non paraben” preservatives in 18 commercial shampoos, finding the actual content of these substances in all samples conformed to their regulatory limits.

Effect of some local plant extracts on lipid stability, organoleptic properties and nutritional value of fish (Alestes baremoze) during sun and smoke drying in Far-North Cameroon

The aim of this study was to evaluate the effect of some local plant extracts on lipid stability, organoleptic properties and the nutritional value of fish (Alestes baremoze) during sun and smoke drying in Far-North region of Cameroon. Three local plants (Xylopia aethiopica fruits, Moringa oleifera leaves and Allium cepa leaves were collected in Maroua town. The fish (Alestes baremoze) was collected in Maga Lake. The eviscerated fish was treated as follows: fish + distilled water (negative control), fish + butylated hydroxytoluene (BHT) at 0.2 g/L (positive control), fish + hydro-ethanolic extracts of each plant at concentrations of 3, 6, 9 and 12 g/L; fish + aqueous extracts of each plant at concentrations of 10, 20, 30 and 40 g/L. After 15 min in the treatment medium, the fish was subjected to smoking or sun drying. Acid, iodine, peroxide, p-anisidine, TOTOX and TBA values of extract oils were determined. Sensory evaluation of smoked and sun-dried fish was determined. The nutritive value of the most preferred smoked and sun-dried treated fish was determined by standard Association of Official Analytical Chemists (AOAC) methods. Results showed that compared to the control, the plant extracts significantly (P < 0.05) protect fish lipid against oxidative and are reflected by: limiting the increase of the acid, peroxide, p-anisidine, TOTOX, TBA values maintaining the high level of the iodine value. Treatment with these extracts significantly increase (P ˂ 0.05), the colour, taste and overall acceptability of treated fish. These plant extracts could also significantly increase (P ˂ 0.05) the proximate and mineral compositions of treated fish.

Determination of health and nutritional benefits of jackfruits (artocarpus heterophyllus)

Jackfruit is one of the tropical fruits uncommonly consumed in Ghana. In this study, the nutritional and health benefits of the jackfruit pulp, together with its bark and leaves, were assessed. The methodology was centered on determining the macro- and micronutrient composition and some potential health benefits of the jackfruit pulps, barks, and leaves. The proximate analytical methods of the Association of Official Analytical Chemists (AOAC 2000) were used to quantify the macronutrients. The micronutrient contents were determined using spectrophotometric and non-spectrophotometric methods. The total phenolic content (TPC), total flavonoids content (TFC), and the β-carotene contents were determined using spectrophotometry as a means of measuring the health benefits of the samples. The crude protein, carbohydrates, and fiber content of the pulp were 1.05±0.06, 17.40±0.36 and 0.46±0.15 g/100 g of fresh fruit sample, respectively. The K, Ca, and P contents of the jackfruit pulps were 422.36±9.60, 69.91±1.66 and 61.17±0.01 mg/100 g of fresh fruit sample, respectively. The TPC, TFC, and β-carotenes content of the pulps were 65.9302±0.0163 mg GAE/100 g, 5.7620±0.0291 mg QE/100 g, and 2.43±0.06 mg/100 g, respectively. The results showed that jackfruit is rich in nutrients including minerals, phytochemicals and in relatively higher amount compared with other fruits.

Edible indigenous fruit trees and shrubs in Tigray, Ethiopia

Edible indigenous fruit trees and shrubs (IFTS) serve as crucial sources of supplementary food and essential nutrients, income/cash, traditional medicine, and various other uses for local communities in Tigray, Ethiopia. However, there is limited documentation on IFTS contributions, production potential, and nutritional compositions. The primary aim of this study was to analyse the production potential, nutritional compositions, and overall contributions of IFTS to rural households. Data were gathered through structured and semi-structured questionnaires from 55 key informants, 15 vendors, and 30 consumers. Nutrient analyses were conducted using triplicate samples following the basic procedures of the Association of Official Analytical Chemists. This study has identified and documented 44 IFTS species. These edible fruits from trees and shrubs are available throughout the year, with peak seasons varying across agroecological zones. Specifically, the lowland, midland, and highland experienced peak fruit availability in November, May, and June, respectively. Fruit yields exhibited significant variation among agroecological zones (P = 0.001), and among growth stages (P < 0.001) within each agroecological zone. Further laboratory analysis revealed that edible IFTS are rich in carbohydrates, crude fiber, crude protein, crude fat, iron (Fe) and zinc (Zn) contents. These nutritional attributes underline the importance of integrating indigenous fruits into the diets of rural and urban households. In conclusion, this research highlights the significant roles played by edible IFTS in the livelihoods of rural households in Tigray, Ethiopia. It also suggests the need for their recognition, conservation, domestication and restoration to enhance food security, income generation, climate adaptation and overall well-being in these communities and beyond.

Comparative Assessment of Soil and Selected Crop in Cement Dust Contaminated Environment in Okpella Area of Edo North, Nigeria

The research was designed to compare the degree of contamination of soil and selected crops by cement dust generated during cement industry activities. Two types of soils were investigated. Soil obtained near the cement industry (OS1) and the soil obtained in a location far from the site of the cement industry (AS2). Only cassava was studied but collected at two different locations. Crop sample collected near the cement industry (OC1) and the one collected at a site far from the cement industry (AC2). The physicochemical properties of the soil samples were determined by analyzing for their percentage nitrogen (N), organic matter (OM), phosphorous (P), potassium (K), electrical conductivity (EC), moisture content (MC), bulk density (BD) and hydrogen ion potential (pH). The proximate composition of the crop samples was determined by analyzing for moisture content, crude protein, ash content, ether extract, crude fibre, dry matter, and carbohydrate. The method used was in accordance with the Association of Official Analytical Chemists’. The samples were characterized for the presence of functional groups using Fourier Transform Infrared (FT-IR) spectroscopy. X-ray Diffractometer (XRD) was used to determine the mineral composition and the heavy metals were determined using the Atomic Absorption Spectroscopy (AAS) method of analysis. The result showed that the percentage of P (28.6±0.01) and K in AS2 was higher than in OS1 (21.6±0.07). The OM in AS2 (2.34±0.01) was higher than in OS1 (2.17±0.01). The values obtained for EC were OS1 (145.7±0.01) and AS2 (115.4±0.28). The concentration of the selected elements did not exceed the maximum allowable amount. Fe has the highest concentration in soil and crop samples. The obtained value for ash content in OC1 was 4.62±0.00 and AC2 was 2.39±0.01. Carbohydrate has 74.4±0.01 in OC1 and 83.4±0.05 in AC2, however moisture content in OC1 was 7.17±0.03 and 6.86±0.03 in AC2. The results also showed the presence of various functional groups and revealed the types of minerals in the samples.

Comprehensive analysis of nutritional composition, mineral content, antimicrobial activity, and theoretical evaluation of phytochemicals in henna (lawsonia inermis) from the Fujairah, United Arab Emirates

Henna (Lawsonia inermis) is a versatile that has been highly regarded for centuries due to its cultural and medicinal significance. Henna boasts a rich array of phytochemicals, each contributing to its numerous therapeutic benefits. This study provided a comprehensive assessment of the henna plant and aimed to explore the potential of phytochemicals as candidates for drug development through various analyses including nutritional and mineral composition, antimicrobial activity, docking analysis, and theoretical ADMET (Absorption, Distribution, Metabolism, Excretion and Toxicity), toxicity, and druglikeness evaluation. The 2 plants Samples collected from 2 different locations in Fujairah emirate exhibited significant amount of Potassium, Magnesium, and calcium, making them a valuable source of essential minerals. The nutritional and mineral content of the sample was measured using international standard methods (Association of Official Analytical Chemists; AOAC 2001.11, AOAC 920.39, AOAC 962.29, and AOAC 942.05). Antimicrobial assessment done by disc diffusion method revealed potent antibacterial activity against Bacillus cereus and Staphylococcus aureus with inhibition of 30-32 mm and 15-20 mm, respectively, Similarly, it shows antifungal activity against Aspergillus flavus and Candida albicans with inhibition of 9-10 mm and 11-12 mm, respectively. Docking analysis provided insights into the interactions between henna phytochemicals and AcuA protein which elucidate the higher interactions of Agrimonolide-6-O-glucopyranoside with binding pocket and surrounding residues with highest binding energy. Similarly, theoretical ADMET, toxicity, and drug likeness analysis highlighted Agrimonolide-6-O-glucopyranoside as a promising compound for drug development as well. These findings hold promise for henna plant and Agrimonolide-6-O- glucopyranoside’s potential use as a natural alternative to conventional antimicrobial agents. However, additional studies are needed to assess the safety, efficacy, and potential mechanisms of action of these phytochemicals in the henna plant extract.

Exploring the nutritional potentials of wild Vigna legume species for neo-domestication prospects

AbstractProjected increases in human population suggest that 70% more food will be needed in the near future, this makes it imperative to search for alternative food and feed sources for human and animal nutrition to feed the exponentially growing human population. According to the FAO 2019 report, the immense challenge of achieving the Zero Hunger target by 2030 is persistent. Exploring the unexplored, refining unrefined traits, cultivating the uncultivated, and popularizing the unpopular remain the most adequate steps proposed by researchers to achieve the domestication of the undomesticated for food and nutrition security. In that line of thought, this study explored the proximate composition of 87 accessions of four wild unexplored Vigna species (V. racemosa, V. ambacensis, V. reticulata, V. vexillata) in order to reveal information leading to their future domestication and utilization. Standard procedures and methods approved by the Association of Official Analytical Chemists were used in carrying out the proximate composition (%protein, %lipid, %fibre, %ash and % moisture and % carbohydrate) of the wild Vigna legumes. The study revealed that the wild Vigna species possess a large variation range of nutrient characteristics which could be exploited in the improvement of domesticated species or guide their domestication. It was also found that some individual wild accessions have higher nutrient, content as compared with domesticated ones which could be advantageous for bio-fortification or domestication. Indications relating to the candidate accessions favourable for domestication, based on the nutrient characteristics were revealed.

Chickpea aquafaba: a systematic review of the different processes for obtaining and their nutritional and technological characteristics

AbstractAquafaba is the residual water from cooking chickpea in water. It has a high gelling ability, allowing it to create stable gels. However, those functional properties depend on the legume composition, genotype, cooking time, pressure, and temperature. This study aimed to evaluate the different processes for obtaining aquafaba and compare their nutritional composition and technological characteristics using a systematic review. The authors performed the systematic review by performing specific search strategies for Scopus, Web of Science, Pubmed, Lilacs, Google Scholar, and ProQuest. A total of 17 studies were analyzed. Of them, 17.64% (n = 3) used the wastewater from canned chickpeas, 17.64% (n = 3) compared the wastewater of canned chickpeas and dry grains, and 58.82% (n = 10) used dry chickpeas. Studies used different methods to analyze the protein content. The most used (n = 5) was the Association of Official Analytical Chemists (AOAC). The aquafaba presented carbohydrates at 2.03–2.59 g/100ml; protein at 0.0.8–2.8 g/100ml; and fat at 0.07–0.1 g/100ml. In general, preparing aquafaba followed: soaking (8–10 h at 4 °C—1 chickpea: 4 water), pressure cooking (30 min—2 chickpea: 3 water), and refrigerating (24h/4 °C). In general, the results showed the following steps to prepare aquafaba: soaking for 8–10 h at 4 °C at the proportion of 1:4 (chickpea:water), pressure cooking for 30 min in the proportion of 2:3 (chickpea: water), and refrigerating 24 h/4 °C. These procedures in a homemade aquafaba presented the best results, considering foam development and higher stability. The aquafaba from canned chickpeas has a higher foam-ability and lower emulsion properties than homemade cooking aquafaba.

Taxonomic characteristics of &lt;i&gt;Spinacia oleracea&lt;/i&gt; L.

The research focused on the taxonomic study and proximate analysis of Spinacia oleracea L. The samples were fixed in formaldehyde acetic acid alcohol, dehydrated in alcohol solutions of 50%, 70% and 90%, sectioned, stained in 2% of Safranin O, counter stained in Alcian blue for 5 minutes, mounted in glycerine and photo-micrographed using Leica WILD MPS 52 camera on Leitz Draplan microscope. The proximate analysis was done using the Association of Official Analytical Chemists’ methods. The result revealed that spinach is glabrous and reach up to 4 to 8ft in length. The foliar organs are simple, ovate to ovate-triangular and alternate to rosette phyllotaxy. Margins are entire to slightly serrated, reticulate veined, up to 5 ± 4 cm in length and 3 ± 2 cm in width. The basal leaves are far larger than the ones towards the apical meristem. The inflorescence has spikelet of actinomorphic flowers pollinated by wind. The fruit is a berry/ drupe. The foliar epidermal study revealed presence of simple elongated, linear and glandular trichomes, and amphistomatic diacytic stomata. Anatomical study showcased a layer of epidermis, hypodermis of 2 to 3 rolls of collenchyma, general cortex of 7 to 10 rolls of parenchyma and the pith of parenchyma. The node is unilacunar with 2 lateral leaf traces and 1 leaf trace median. The proximate compositions of: 0.19 ± 0.00 Carbohydrate (%); 3.75 ± 0.00 Protein (%); 0.02 ± 0.00 Lipid (%); 94.74 ± 0.36 Moisture (%) content; 0.62 ± 0.32 Fiber (%) and 1.18 ± 0.03 Ash (%). These information would assist for further delimitation of the species.

Determination of Seven Human Milk Oligosaccharides (HMOs) in Infant Formula and Adult Nutritionals: First Action 2022.07.

Human milk oligosaccharides (HMOs) are important components of breast milk and may be responsible for some of the benefits of breastfeeding, including resistance to infections and the development of a healthy gut microbiota. Selected HMOs are now available for addition to infant formula, and suitable methods to control the dosing rate are needed. To develop and validate a suitable method for the analysis of HMOs in infant formula. A method was developed for the determination of seven human milk oligosaccharides (2'-fucosyllactose, 3-fucosyllactose, 3'-sialyllactose, 6'-sialyllactose (6'SL), 2',3-difucosyllactose, lacto-N-tetraose (LNT), lacto-N-neotetraose (LNnT)) in infant formula and adult nutritionals. The oligosaccharides are labeled at their reducing end with 2-aminobenzamide, separated by liquid chromatography and detected using a fluorescence detector. Maltodextrins are enzymatically hydrolyzed before analysis to prevent potential interference; likewise, an optional β-galactosidase treatment can be used to remove β-galactooligosaccharides. Fructooligosaccharides or polydextrose do not generally interfere with the analysis. The method has been validated in a single laboratory on infant formula and adult nutritionals. The seven HMOs were spiked into eight matrixes at three or four spike levels, giving a total of 176 data points. Recoveries were in the range of 90.9-109% in all cases except at the lowest spike level in one matrix (elemental formula), where the LNT recovery was 113%, the LNnT recovery was 111%, and the 6'SL recovery was 121%. Relative repeatabilities (RSD(r)) were in the range of 0.1-4.2%. The performance is generally within the requirements outlined in the Standard Method Performance Requirements (SMPR®) published by AOAC INTERNATIONAL. The method developed is suitable for the determination of seven HMOs in infant formula and demonstrated good performance during single-laboratory validation. A method has been developed that is suitable for the determination of seven HMOs in infant formula.