BackgroundChildren's vaccination mainly depends on their parents' decisions. Therefore, it is important to understand parents' acceptance and attitudes toward vaccinating their children against COVID-19. AimThe study aimed to assess the pediatric nurses' acceptance and attitudes toward COVID-19 vaccines for their children at Assiut University Children Hospital. Materials and methodA cross-sectional descriptive design was used on a convenience sample of 270 nurses who worked at Assiut University Children Hospital. ToolsTool one involved the demographic characteristics of pediatric nurses and their children. Tool two consisted of pediatric nurses' acceptance and attitudes toward COVID-19 vaccines for their children: ResultsThe results indicated that 79.3% of pediatric nurses had higher acceptance of vaccines and immunization of their children. Results also revealed that 80.0% of pediatric nurses had positive attitudes toward vaccines and their children's immunizations. ConclusionIt was concluded that most of pediatric nurses had high acceptance and positive attitudes toward vaccines and immunization of their children. Also, it was clarified that there was a positive statistically significant correlation between nurses' acceptance of vaccines and immunization of their children and their attitudes. RecommendationsContinuing education and tailored intervention should be implemented to correct misinformation and negative conceptions about the safety of vaccines for children.