Linkage Assembly Research Articles

Selenium(II)-Nitrogen Exchange (SeNEx)Chemistry: A Good Chemistry Suitable for Nanomole-Scale Parallel Synthesis, DNA-encoded Library Synthesis, and Bioconjugation.

The continuous development of click reactions with new connecting linkage is crucial for advancing the frontiers of click chemistry. Selenium-nitrogen exchange (SeNEx) chemistry, a versatile chemistry in click chemistry, represents an all-encompassing term for nucleophilic substitution events that replace nitrogen at an electrophilic selenium(II) center, enabling the flexible and efficient assembly of linkages around a Se(II) core. Several SeNEx chemistries have been developed inspired by the biochemical reaction between Ebselen and cysteine residue, and demonstrated significant potential in on-plate nanomole-scale parallel synthesis, selenium-containing DNA-encoded library (SeDEL) synthesis, as well as peptide and protein bioconjugation. This concept aims to present the origins, advancements, and applications of selenium(II)-nitrogen exchange (SeNEx) chemistry while also outlining the potential directions for future research in this field.

Novel genome-wide significant germline genetic variants associated with venous thrombo-embolism (VTE) and arterial embolism (ATE) risk in patients with ALK and ROS1 fusion non-small cell lung cancer (NSCLC).

12092 Background: In a recent meta-analysis, non small cell lung cancers (NSCLC) with ALK or ROS1 fusions had higher rates of VTE compared to KRAS or EGFR mutant NSCLC, and were associated with increased ATE risk, particularly around diagnosis. The underlying mechanisms of how these somatic fusions affect thrombosis remain unclear. We examined clinico-demographic and germline genetic factors associated with VTE and ATE in NSCLC patients with ALK/ROS1 fusions in this first comprehensive genome wide association analysis (GWAS). Methods: In this prospective cohort, germline DNA from whole blood was obtained from 150 patients with ALK fusions and 32 with ROS1 fusions at Princess Margaret Cancer Centre (recruited 2014- 2023). Clinico-demographic, treatment and outcome data were collected from electronic medical charts. Overall survival (OS) by VTE or ATE status was assessed via Cox regression, treating ATE and VTE as time-varying covariates. Genotyping utilized the Infinium Global Screening Array (v3.0 Illumina); quality-control removed 3 patients from final analysis. Using linear regression, outcomes were weighted; no VTE/ATE events (0), one VTE/ATE event (1), or multiple ATE/VTEs (>2). Global significance was set at p < 5 x10-8 and adjusted for age, sex, body mass index (BMI), and ethnic/population stratification (top 3 principal components). Results: There were 97 females (54%) and 82 males (46%), mean age was 57.4 years, 70% had stage 4 disease, 96% adenocarcinoma; 35% experienced VTE/ATE at any time; 13% had 2+ VTE/ATE events. For those with VTE, 32 scored 1, 22 scored 2 and 6 scored 3 on Khorana score. Higher BMI was a significant risk factor for VTE/ATE (adjusted odds ratio 1.59 per 5-unit increase, p=0.01). Shorter OS was observed in patients with VTE (Hazard ratio (HR)=3.15, p<0.001) and ATE (HR=2.51, p=0.036), compared to those without. Two novel GWAS gene peaks on the Manhattan plot (previously not reported to be associated with VTE/ATE) had globally significant associations with risk of VTE/ATE: at Chromosome 6q15 (intergenic region between RNGTT and LOC101928936; 5/10 top variants with top risk-allele p=3.594E-09) and at 15p22 ( TLN2 gene; 2/10 top variants p=1.095E-08). TLN2 is a cytoskeletal protein involved in the assembly of actin filaments and linkages with extracellular matrices. Additional identified variants potentially associated with VTE/ATE in ALK/ROS1 fusion patients were found in: CTBP2, NALCN, WDR7, PAX7, ZNF385D, SORBS2, and CSMD1. Conclusions: Two novel globally significant variants, associated with VTE/ATE, are unique to patients with ALK/ROS1 fusion NSCLC. If validated, these biomarkers may help identify ALK/ROS1 patients who are at highest risk of VTE/ATE who may benefit from prophylactic anticoagulation. Further investigation and clinical evaluation are warranted.

Pre-conceptual design of the steering mirror for the DEMO electron cyclotron heating system

An Electron Cyclotron (EC) Heating and Current Drive (HCD) system will be installed in the EU DEMO Tokamak to provide plasma heating and stabilization by coupling up to 130 MW of mm-wave power into specific regions of the plasma. The mm-waves will be generated by gyrotrons and propagated through a quasi-optical multi-beam mirror system (MBMS), and subsequently through corrugated waveguides, to launchers located at dedicated Equatorial Ports (EPs). The present design of the launcher features two mid steering antennas (MSAs) for plasma stabilization each consisting of three-beam layouts and steering mirrors positioned at the upper and lower locations of the EP. The proposed concept for the steering mirrors operates on the pantograph principle, a mechanical assembly of parallel linkages, which translates the movement produced by pneumatic actuators into an exact angular rotation of the mirror around an offset virtual axis. This design uses flexures with specific structural compliance as mechanical joints resulting in the desired movement of the linkage system while avoiding the tribological difficulties associated with mechanisms operating under vacuum. This paper describes the present status of the DEMO EC HCD steering mirror as well as the analyses performed to validate this design.

A Load Control Strategy for Stable Operation of Free-Piston Electromechanical Hybrid Power System

Abstract The free-piston electromechanical hybrid power system (FEHS) affords the advantages of a simple construction and high thermal efficiency due to the removal of the crankshaft. However, the unrestricted trajectory of the piston linkage assembly (PLA) also gives rise to challenges for stable operation during the process of startup or operation. In order to realize the stable operation of free-piston electromechanical hybrid power system, this paper proposed a load control strategy. First, a dynamic model is established through thermodynamic and electromagnetic theory, and its effectiveness is verified by experiment and simulation. On this basis, a coupling load control model based on linear active disturbance rejection control (ADRC) is developed. The reliability of the proposed load control strategy is validated under different interference fluctuations. The simulation results demonstrate that no matter whether the interference occurs during the startup process or the operation process, the proposed control strategy exerts effective limiting function over the piston linkage assembly and maintain its stable operation. Moreover, compared with the proportion integral differential (PID) control strategy, the proposed strategy exhibits faster response times and a smoother startup process. The compression ratio fluctuation range was reduced from 0.1 to 0.001, and the control accuracy has been greatly improved.

Ex situ Generation of Thiazyl Trifluoride (NSF3) as a Gaseous SuFEx Hub**

AbstractSulfur(VI)‐fluoride exchange (SuFEx) chemistry, an all‐encompassing term for substitution events that replace fluoride at an electrophilic sulfur(VI), enables the rapid and flexible assembly of linkages around a SVI core. Although a myriad of nucleophiles and applications works very well with the SuFEx concept, the electrophile design has remained largely SO2‐based. Here, we introduce S≡N‐based fluorosulfur(VI) reagents to the realm of SuFEx chemistry. Thiazyl trifluoride (NSF3) gas is shown to serve as an excellent parent compound and SuFEx hub to efficiently synthesize mono‐ and disubstituted fluorothiazynes in an ex situ generation workflow. Gaseous NSF3 was evolved from commercial reagents in a nearly quantitative fashion at ambient conditions. Moreover, the mono‐substituted thiazynes could be extended further as SuFEx handles and be engaged in the synthesis of unsymmetrically disubstituted thiazynes. These results provide valuable insights into the versatility of these understudied sulfur functionalities paving the way for future applications.

Ex situ Generation of Thiazyl Trifluoride (NSF3 ) as a Gaseous SuFEx Hub.

Sulfur(VI)-fluoride exchange (SuFEx) chemistry, an all-encompassing term for substitution events that replace fluoride at an electrophilic sulfur(VI), enables the rapid and flexible assembly of linkages around a SVI core. Although a myriad of nucleophiles and applications works very well with the SuFEx concept, the electrophile design has remained largely SO2 -based. Here, we introduce S≡N-based fluorosulfur(VI) reagents to the realm of SuFEx chemistry. Thiazyl trifluoride (NSF3 ) gas is shown to serve as an excellent parent compound and SuFEx hub to efficiently synthesize mono- and disubstituted fluorothiazynes in an ex situ generation workflow. Gaseous NSF3 was evolved from commercial reagents in a nearly quantitative fashion at ambient conditions. Moreover, the mono-substituted thiazynes could be extended further as SuFEx handles and be engaged in the synthesis of unsymmetrically disubstituted thiazynes. These results provide valuable insights into the versatility of these understudied sulfur functionalities paving the way for future applications.

Modular Synthesis of a Tridecasaccharide Motif of Bacteroides vulgatus Lipopolysaccharides against Inflammatory Bowel Diseases through an Orthogonal One-Pot Glycosylation Strategy.

Lipopolysaccharides from Bacteroides vulgatus represent interesting targets for the treatment of inflammatory bowel diseases. However, efficient access to long, branched and complex lipopolysaccharides remains challenging. Herein, we report the modular synthesis of a tridecasaccharide from Bacteroides vulgates through an orthogonal one-pot glycosylation strategy based on glycosyl ortho-(1-phenylvinyl)benzoates, which avoids the issues of thioglycoside-based one-pot synthesis. Our approach also features: 1) 5,7-O-di-tert-butylsilylene-directed glycosylation for stereoselective construction of the α-Kdo linkage; 2) hydrogen-bond-mediated aglycone delivery for the stereoselective formation of β-mannosidic bonds; 3) remote anchimeric assistance for stereoselective assembly of the α-fucosyl linkage; 4) several orthogonal one-pot synthetic steps and strategic use of orthogonal protecting groups to streamline oligosaccharide assembly; 5) convergent [1+6+6] one-pot synthesis of the target.

Thick-panel origami-based parabolic cylindrical antenna

This paper proposes a novel parabolic cylindrical antenna with great folding efficiency based on two symmetrically arranged Miura-ori thick-panel origami patterns and the removal of partial material from each panel. A design rule for the transition from the given parabolic equation to the geometric parameters of the thick-panel origami is defined. To ensure that the deployable structure has one degree of freedom (DOF), specific connectors between the two thick-panel origami patterns are designed from spherical 4R linkages and Bennett linkages, which are verified by kinematic analysis based on the assembly of linkages corresponding to the thick-panel origami pattern. Two joint-removing techniques based on the kinematic equivalence of linkages are constructed to obtain the continuous and smooth parabolic cylindrical surface and maintain the one-DOF property. The deployed/folded ratio influencing factors are fully studied, finding that a small panel thickness and a large origami pattern sector angle are necessary to obtain a high deployed/folded ratio. Finally, a prototype of the deployable structure is manufactured to verify and demonstrate its excellent performance.

Thick-Panel Origami Tubes With Hexagonal Cross-Sections

Abstract Rigidly foldable origami tubes can be kinematically regarded as assemblies of spherical linkages. They have exhibited excellent properties for deployable structures. Yet, for the engineering applications, the corresponding thick-panel forms have to be designed. In this paper, the spherical 4R linkages in tubes with hexagonal cross-sections are partially replaced by spatial linkages, leading to a method to construct the thick-panel tubes, which can reproduce kinematic motions equivalent to those realized using zero-thickness origami. Based on the D–H matrix method, the rotational symmetric and symmetric tubes are introduced, together with their four types of vertexes, where the specific spherical 4R linkages are replaced by Bennett and Bricard linkages to obtain the thick-panel foldable tubes. The approach can be applied to multilayered tubes with a straight or curved profile, whose manufacture can be simplified by removing extra links. The results can be readily utilized to the design of deployable tubular structures whose thickness cannot be disregarded.

Motion singularity analysis of the thick-panel kirigami

Thick-panel kirigami, constructed by cutting open creases on origami structures, can be applied to deployable structures with different Gaussian curvatures. By converting creases to rotational hinges, thick-panel kirigami can be treated as the assembly of spatial linkage, which provides accurate and precise deployment motion. Compared with origami, kirigami structures are able to optimize jagged deployed surfaces by geometry parameter adjustment. However, replacing several creases with slits in structures may lead to shortcomings in kinematics, i.e., motion singularity. In this study, motion singularity of thick-panel kirigami structure is discussed. Starting with kirigami units, bifurcation paths are detected and followed by the predictor-corrector numerical method. We then check the corresponding extension kirigami arrays and explore their complex bifurcation behaviors. The result indicates that the bifurcation motion paths within kirigami structures may lead to incomplete deployment. Physical prototypes are carried out, which successfully validate the singularity analysis. Also, motor- and spring-based hinges are arranged for driving, and the redundant actuator method is considered to avoid bifurcations and accomplish the full deployment. The findings are applicable to the design of kirigami-inspired deployable structures for engineering applications in the future.

Kirigami-inspired thick-panel deployable structures

Thick-panel origami has been studied extensively. One of the approaches converts origami creases to simple rotational connections and treats thick-panel origami as assembly of spatial linkages. While structures developed by this approach have one degree of freedom that provide accurate and precise deployment, they often expand to jagged surfaces. In this study, a kirigami-inspired approach, which selectively cuts open some creases in origami patterns, is presented to create deployable surface structures with completely flat surfaces. Starting with a two-vertex origami pattern, we use the theory of spatial linkages to investigate the foldabilities when different mountain–valley assignments are adopted. Combined with a parametric study on the panel thicknesses, we identify a family of deployable two-vertex thick-panel kirigami structures composed of panels of uniform thickness. The slits of the kirigami close completely once deployed. They are subsequently tessellated to form large deployable flat arrays while the kinematic properties are retained. The findings are beneficial for designing deployable structures for engineering applications involving flat panels, e.g., solar panels, shelters, and solid surface antennas.

Cell Wall Polysaccharide Composition of Grafted 'Liberty' Watermelon With Reduced Incidence of Hollow Heart Defect.

Grafting watermelon scions to interspecific squash hybrids has been found to increase fruit firmness. Triploid (seedless) watermelon are prone to hollow heart (HH), an internal fruit disorder characterized by a crack in the placental tissue expanding to a cavity. Although watermelon with lower tissue firmness tend to have a higher HH incidence, associated differences in cell wall polysaccharide composition are unknown. Grafting “Liberty” watermelon to “Carnivor” (interspecific hybrid rootstock, C. moschata × C. maxima) reduced HH 39% and increased tissue firmness by 3 N. Fruit with and without severe HH from both grafted and non-grafted plants were analyzed to determine differences in cell wall polysaccharides associated with grafting and HH. Alcohol insoluble residues (AIR) were sequentially extracted from placental tissue to yield water soluble (WSF), carbonate soluble (CSF), alkali soluble (ASF), or unextractable (UNX) pectic fractions. The CSF was lower in fruit with HH (24.5%) compared to those without HH (27.1%). AIRs were also reduced, hydrolyzed, and acetylated for GC-MS analysis of monosaccharide composition, and a portion of each AIR was methylated prior to hydrolysis and acetylation to produce partially methylated alditol acetates for polysaccharide linkage assembly. No differences in degree of methylation or galacturonic and glucuronic acid concentrations were found. Glucose and galactose were in highest abundance at 75.9 and 82.4 μg⋅mg–1 AIR, respectively, followed by xylose and arabinose (29.3 and 22.0 μg⋅mg–1). Mannose was higher in fruit with HH (p < 0.05) and xylose was highest in fruit from grafted plants (p < 0.05). Mannose is primarily found in heteromannan and rhamnogalacturonan I side chains, while xylose is found in xylogalacturonan or heteroxylan. In watermelon, 34 carbohydrate linkages were identified with galactose, glucose, and arabinose linkages in highest abundance. This represents the most comprehensive polysaccharide linkage analysis to date for watermelon, including the identification of several new linkages. However, total pectin and cell wall composition data could not explain the increased tissue firmness observed in fruit from grafted plants. Nonetheless, grafting onto the interspecific hybrid rootstock decreased the incidence of HH and can be a useful method for growers using HH susceptible cultivars.

Long‐range assembly of sequences helps to unravel the genome structure and small variation of the wheat–Haynaldia villosa translocated chromosome 6VS.6AL

SummaryGenomics studies in wild species of wheat have been limited due to the lack of references; however, new technologies and bioinformatics tools have much potential to promote genomic research. The wheat–Haynaldia villosa translocation line T6VS·6AL has been widely used as a backbone parent of wheat breeding in China. Therefore, revealing the genome structure of translocation chromosome 6VS·6AL will clarify how this chromosome formed and will help to determine how it affects agronomic traits. In this study, chromosome flow sorting, NGS sequencing and Chicago long‐range linkage assembly were innovatively used to produce the assembled sequences of 6VS·6AL, and gene prediction and genome structure characterization at the molecular level were effectively performed. The analysis discovered that the short arm of 6VS·6AL was actually composed of a large distal segment of 6VS, a small proximal segment of 6AS and the centromere of 6A, while the collinear region in 6VS corresponding to 230–260 Mb of 6AS‐Ta was deleted when the recombination between 6VS and 6AS occurred. In addition to the molecular mechanism of the increased grain weight and enhanced spike length produced by the translocation chromosome, it may be correlated with missing GW2‐V and an evolved NRT‐V cluster. Moreover, a fine physical bin map of 6VS was constructed by the high‐throughput developed 6VS‐specific InDel markers and a series of newly identified small fragment translocation lines involving 6VS. This study will provide essential information for mining of new alien genes carried by the 6VS·6AL translocation chromosome.

TRIP12 promotes small-molecule-induced degradation through K29/K48-branched ubiquitin chains

Targeted protein degradation is an emerging therapeutic paradigm. Small-molecule degraders such as proteolysis-targeting chimeras (PROTACs) induce the degradation of neo-substrates by hijacking E3 ubiquitin ligases. Although ubiquitylation of endogenous substrates has been extensively studied, the mechanism underlying forced degradation of neo-substrates is less well understood. We found that the ubiquitin ligase TRIP12 promotes PROTAC-induced and CRL2VHL-mediated degradation of BRD4 but is dispensable for the degradation of the endogenous CRL2VHL substrate HIF-1α. TRIP12 associates with BRD4 via CRL2VHL and specifically assembles K29-linked ubiquitin chains, facilitating the formation of K29/K48-branched ubiquitin chains and accelerating the assembly of K48 linkage by CRL2VHL. Consequently, TRIP12 promotes the PROTAC-induced apoptotic response. TRIP12 also supports the efficiency of other degraders that target CRABP2 or TRIM24 or recruit CRBN. These observations define TRIP12 and K29/K48-branched ubiquitin chains as accelerators of PROTAC-directed targeted protein degradation, revealing a cooperative mechanism of branched ubiquitin chain assembly unique to the degradation of neo-substrates.

Mobile assemblies of four-spherical-4R-integrated linkages and the associated four-crease-integrated rigid origami patterns

Rigid origami, which can be regarded as assemblies of spherical linkages, inspires a new paradigm of design for mechanical metamaterials and deployable structural systems with large deployable ratio. In this paper, the kinematic properties of assemblies of spherical 4R linkages are studied, and new rigid origami patterns are obtained. Kinematics of a single loop spherical 4R linkage is firstly presented, and by assigning four specified values for the four twist angles, and defining four pairs of specified input and output angles, 16 maps between the input and output angles are obtained resulting in 256 types of special spherical 4R linkages. By merging four identical spherical 4R linkages into a single loop, three basic mobile assemblies are constructed and the one-degree-of-freedom kinematic compatibility condition is formulated. Through alteration of the four vertex linkages in the three basic assemblies, variations of the basic assemblies are generated. Consequently, by adding further geometric conditions, novel rigid origami patterns are obtained leading to the tessellation of the spherical-4R-linkage-integrated assemblies. Hence, this paper provides a novel approach for generating new rigid origami patterns which can lead to the development of foldable structures and tessellations with potential applications in robotics, smart architectures, mechanical metamaterials and space exploration.

Maintenance and inspection as risk factors in helicopter accidents: Analysis and recommendations.

In this work, we establish that maintenance and inspection are a risk factor in helicopter accidents. Between 2005 and 2015, flawed maintenance and inspection were causal factors in 14% to 21% of helicopter accidents in the U.S. civil fleet. For these maintenance-related accidents, we examined the incubation time from when the maintenance error was committed to the time when it resulted in an accident. We found a significant clustering of maintenance accidents within a short number of flight-hours after maintenance was performed. Of these accidents, 31% of these accidents occurred within the first 10 flight-hours. This is reminiscent of infant mortality in reliability engineering, and we characterized it as maintenance error infant mortality. The last quartile of maintenance-related accidents occurred after 60 flight-hours following maintenance and inspection. We then examined the “physics of failures” underlying maintenance-related accidents and analyzed the prevalence of different types of maintenance errors in helicopter accidents. We found, for instance, that the improper or incomplete (re)assembly or installation of a part category accounted for the majority of maintenance errors with 57% of such cases, and within this category, the incorrect torquing of the B-nut and incomplete assembly of critical linkages were the most prevalent maintenance errors. We also found that within the failure to perform a required preventive maintenance and inspection task category, the majority of the maintenance programs were not executed in compliance with federal regulations, nor with the manufacturer maintenance plan. Maintenance-related accidents are particularly hurtful for the rotorcraft community, and they can be eliminated. This is a reachable objective when technical competence meets organizational proficiency and the collective will of all the stakeholders in this community. We conclude with a set of recommendations based on our findings, which borrow from the ideas underlying the defense-in-depth safety principle to address this disquieting problem.

Multi-allelic QTL analysis of protein content in a bi-parental population of cultivated tetraploid potato

Protein content is a key quality trait for the potato starch industry. The objective of this study was to identify allele-specific quantitative trait loci (QTLs) for tuber protein content in cultivated potato (Solanum tuberosum L.) at the tetraploid level. We analysed 496 full-sib F1 clones in a 3-year field trial to dissect the complex genetic architecture of soluble tuber protein content. Genotypic data from a 60K single nucleotide polymorphism (SNP) array was used for SNP dosage scoring, constructing homologue specific linkage maps and assembly of a dense integrated chromosomal linkage map. From the integrated map, probabilistic multi-locus identity-by-descent (IBD) haplotypes (alleles) were estimated and used to detect associations between the IBD haplotypes and the phenotypic trait values. Moderate levels of trait heritability were estimated between 40 and 74% that correspond with previous studies. Our contemporary naive analysis identified potential additive QTLs on chromosomes 2, 3, 5 (top arm) and 9 across the years. Moreover, cofactor QTL analysis identified two masked QTLs on chromosomes 1 and 5 (lower arm). The QTLs on chromosomes 2, 5 (lower arm) and 9 are reported here for the first time. The QTLs that we identified on chromosomes 1, 3 and 5 (top arm) show overlap with previous studies for protein content in potato. Collectively the naive QTLs explained 12 to 17% of the phenotypic variance. The underlying alleles of the QTLs provided both positive and negative effects on the phenotype. Our work uncovers the complex genetic architecture of this trait and describes potential breeding strategies for improvement. As protein has emerged as a high-value component from industrial potato starch production, the dissection of the genetic architecture and subsequent improvement of this trait by breeding has great economic and environmental relevance.

Optimisation of brake pedal linkage: a comparative analysis towards material saving using CAE tools

This work is concerned with the design optimisation of brake pedal linkage. An existing model of brake pedal linkage was analysed through finite element analysis module of ANSYS. The target outcomes were observed under defined loading and boundary conditions to provide a realistic analysis environment. The maximum deflection, von Mises stress, etc. were determined for maximum load through FEA. The maximum stress prone areas of the assembly as well as parts under high stress were concentrated. To optimise existing model, the stress free volume of the material from the linkage assembly has been targeted. The CAD model of the new design of linkage is modelled using CATIA features. Keeping in view the design concepts of mechanical components this stress free volume of the material has been removed to make it light weight without compromising with the strength of linkage. The optimised model has been analysed through ANSYS again to observe its outcomes. The prime objective of this work is material saving and related material cost minimisation.

Optimisation of brake pedal linkage: a comparative analysis towards material saving using CAE tools

This work is concerned with the design optimisation of brake pedal linkage. An existing model of brake pedal linkage was analysed through finite element analysis module of ANSYS. The target outcomes were observed under defined loading and boundary conditions to provide a realistic analysis environment. The maximum deflection, von Mises stress, etc. were determined for maximum load through FEA. The maximum stress prone areas of the assembly as well as parts under high stress were concentrated. To optimise existing model, the stress free volume of the material from the linkage assembly has been targeted. The CAD model of the new design of linkage is modelled using CATIA features. Keeping in view the design concepts of mechanical components this stress free volume of the material has been removed to make it light weight without compromising with the strength of linkage. The optimised model has been analysed through ANSYS again to observe its outcomes. The prime objective of this work is material saving and related material cost minimisation.

The diamond thick-panel origami and the corresponding mobile assemblies of plane-symmetric Bricard linkages

Thick-panel origami and mobile assembly of spatial linkages are of superiorly foldable properties and therefore have great potential for engineering applications, such as solar panels and antennas. This paper sets up the kinematic equivalence between the diamond thick-panel origami and mobile assembly of plane-symmetric Bricard linkages. Through the compatibility analysis of linkage assembly derived from the diamond thick-panel origami pattern, two general cases of mobile assemblies of plane-symmetric Bricard linkages are discovered. Furthermore, inspired by one of the newly-found mobile assemblies, the sector angle and thickness of diamond thick-panel origami pattern can be varied. The new diamond thick-panel origami patterns with flat unfolded profiles and/or spirally folded configuration are invented, and the graded one can be extended without physical interference. The work proposes a bridge between diamond thick-panel origami and the assembly of plane-symmetric Bricard linkages, and facilitates their applications.