Macaca Assamensis Research Articles

Phylogeography of the Sinica Group of Macaques in the Himalayas: Taxonomic and Evolutionary Implications.

Owing to the taxonomic incongruence between the morphological features and genetic relationships of the sinica group of macaques (genus Macaca), the taxonomy of this macaque group has remained inconclusive. We aimed to resolve the taxonomic quandary and improve our understanding of the historical biogeography of the group by including macaque DNA samples from previously unsampled areas in the Himalayas. We sequenced and analyzed three mitochondrial DNA loci [cytochrome b (CYTB), cytochrome oxidase subunit 1 (COI) and D-loop; 2898 bp] for sequence polymorphism, phylogenetics, species delimitation, and ancestral area reconstruction. We confirmed the occurrence of Arunachal macaque (Macaca munzala) on the southern slopes of the Eastern Himalayas in the Xizang Zizhiqu (Tibet Autonomous Region) of China. The results revealed that the sinica group of macaques is a parapatric species group composed of seven distinct species. Phylogenetic and species delimitation analyses revealed that the two previously considered subspecies of Assamese macaques (the eastern subspecies M. assamensis assamensis and the western subspecies M. a. pelops) are two distinct species. The eastern Assamese macaque is a sister species to the Tibetan macaque, whereas the western Assamese macaque and Arunachal macaque are the closest genetic sister species. The sinica group of macaques underwent five vicariance and seven dispersal radiations in the past, which mainly coincided with the Quaternary climatic oscillations between the late Pliocene and the late Pleistocene. By integrating our phylogenetic and ancestral area reconstruction results with findings from previous paleontological and molecular studies, we propose a robust hypothesis about the phylogeography of the sinica group of macaques.

Anthropogenic disturbance and competitive avoidance shape the coexistence pattern of macaque species in tropical forests

AbstractThe factors that enable the coexistence of closely related species remain a major question in ecology, particularly in human‐disturbed habitats. The effects of anthropogenic disturbance and interspecific competition can exacerbate the decline in populations of competing species. The adoption of different strategies in responding to anthropogenic disturbances and competitive avoidances may create opportunities for competing species to coexist. However, few studies have explored how disturbance and competition interact to shape species coexistence. In this study, we conducted long‐term and large‐scale camera trap surveys comprising 540 sampling sites from 2017 to 2021 at Xishuangbanna, southwestern China, and deployed a spatiotemporal analysis framework to determine the effect of anthropogenic disturbances and competitive avoidances on the coexistence of three sympatric macaque species: Assamese macaque (Macaca assamensis; MA), northern pig‐tailed macaque (M. leonina; ML), and rhesus macaque (M. mulatta; MM). Macaque species exhibited diverse responses to different types of anthropogenic disturbances. The occurrence probability of MM was positively associated with distance to road and relative abundance of human occurrence, and negatively associated with distance to cropland, which reduces the likelihood of sympatry between MM and the other two species due to their opposing responses to road, cropland, and human occurrence. Conversely, the similar responses to road and cropland increase the sympatry between MA and ML. Three macaque species did not avoid each other through shifting space use or their overall daily activity pattern. However, they delayed using the shared site after other species used it to avoid confrontation. We provide evidence that (1) the spatial co‐occurrence pattern of sympatric macaque species was determined by anthropogenic disturbances rather than by competitive spatial avoidance and (2) fine‐scale temporary avoidance is the strategy to alleviate their interspecific competition. These results enhance our understanding of the underlying mechanisms leading to species coexistence of nonhuman primates in human‐disturbed habitats.

Evolution of the CYP2B subfamily gene in primates and its relationship to diet

Herbivores rely heavily on liver enzymes to deal with plant secondary metabolites (PSMs) due to their consumption on plant-based diets, but only a small amount of enzymes have been described based on the genomic level. It is suggested that a multigene subfamily, cytochrome P450 2B (CYP2B), involved in PSMs processing. However, CYP2B subfamily genes of only several rodent species have been characterized, and the copies of CYP2B gene have been compared between the specialist and generalist. Here we investigated the phylogeny of CYP2B subfamily genes in primates with known genome sequences, and related the number of gene to the diet. Furthermore, we compared the CYP2B subfamily genes in two closely related species, Macaca assamensis and Ma. mulatta, which can be viewed as specialist (mainly feeds on Bonia saxatilis) and generalist, respectively, to examine whether the difference of gene number or sequence diversity exists between these two species. The result showed that, most primates have only one CYP2B subfamily gene, although several herbivores have two copies; and both Ma. assamensis and Ma. mulatta have one CYP2B subfamily gene, each with 8 SNPs in them, which is mainly because that multiple SNPs can lead to higher diversity, and further can help deal with various toxins. Physicochemical property prediction indicated that some of these SNPs may cause radical variations between the gene functions. This study is a comprehensive exploration on the evolution of a detoxification enzyme gene subfamily in the primate group, and provides an initial genetic foundation to explore how the closely related species respond to diet from the perspective of P450.

Distribution records of Assamese macaques (Macaca assamensis pelops) from the Bheri River Basin in western Nepal

The Assamese macaque (Macaca assamensis) population in Nepal is one of the least studied mammals. It is legally protected by the National Parks and Wildlife Conservation Act 1973; however, its population status, distribution and conservation threats are ill-documented. The species is thought to have a disjunct distribution having limited information about its occurrence in mid-western and far-western Nepal. This study surveyed the Assamese macaques in the Bheri River Basin of western Nepal and recorded their distribution in the basin for the first time. A total of 117 individuals were observed belonging to six different troops with an average troop size of 19.5 individuals. The elevational range of the distribution ranged between 1132 m and 1840 m asl. The Assamese macaque individuals from the Bheri River Basin are much darker and bear longer relative tail lengths than those of eastern and central Nepal. Further explorations and a detailed assessment of conservation threats in western Nepal are warranted.

The continued presence of Assamese macaques Macaca assamensis (McClelland, 1840) at the lowest elevational record for Nepal

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Molecular Detection and Characterization of Hemotropic Mycoplasma in Assamese Macaques (Macaca assamensis) of Northern Thailand.

Hemotropic mycoplasmas, also known as hemoplasmas, are parasitic bacteria that infect red blood cells, potentially leading to varying degrees of anemia across numerous mammalian species, including nonhuman primates. The present study aims to investigate the prevalence of hemoplasma infection and identify the species involved among free-ranging Assamese macaques (Macaca assamensis) inhabiting northern Thailand. A total of 133 blood samples were collected from Assamese macaques in Chiang Rai province, Thailand, and subjected to screening for hemoplasma infection utilizing nested PCR amplification targeting the 16S rRNA gene. Positive samples were subsequently analyzed through nucleotide sequencing and phylogenetic analysis for putative species identification. Current study results revealed that 17.3% (23/133; 95% CI 11.29-24.81) of Assamese macaques tested positive for hemoplasma infection using the nested PCR assay. Partial 16S rRNA sequences derived from hemoplasma isolates in Assamese macaques exhibited 99% homology, forming a cluster within the same phylogenetic clade as "Candidatus Mycoplasma haematomacacae," previously identified in long-tailed macaques, rhesus macaques, and Japanese macaques. These findings suggest the presence of "Ca. M. haematomacacae" not only in long-tailed macaques and rhesus macaques but also in Assamese macaques in Thailand. To our knowledge, this marks the first molecular detection of "Ca. M. haematomacacae" in Assamese macaques in Thailand. These results hold significance as they enhance our understanding of hemoplasma infection distribution among macaque populations in Thailand.

Social network shrinking is explained by active and passive effects but not increasing selectivity with age in wild macaques.

Evidence of social disengagement, network narrowing and social selectivity with advancing age in several non-human animals challenges our understanding of the causes of social ageing. Natural animal populations are needed to test whether social ageing and selectivity occur under natural predation and extrinsic mortality pressures, and longitudinal studies are particularly valuable to disentangle the contribution of within-individual ageing from the demographic processes that shape social ageing at the population level. Data on wild Assamese macaques (Macaca assamensis) were collected between 2013 and 2020 at the Phu Khieo Wildlife Sanctuary, Thailand. We investigated the social behaviour of 61 adult females observed for 13 270 h to test several mechanistic hypotheses of social ageing and evaluated the consistency between patterns from mixed-longitudinal and within-individual analyses. With advancing age, females reduced the size of their social network, which could not be explained by an overall increase in the time spent alone, but by an age-related decline in mostly active, but also passive, behaviour, best demonstrated by within-individual analyses. A selective tendency to approach preferred partners was maintained into old age but did not increase. Our results contribute to our understanding of the driver of social ageing in natural animal populations and suggest that social disengagement and selectivity follow independent trajectories during ageing.

Seasonal patterns of the gut microbiota in the Assamese macaques (Macaca assamensis) in a limestone forest in Guangxi, China

Seasonal patterns of the gut microbiota in the Assamese macaques (Macaca assamensis) in a limestone forest in Guangxi, China

Projected distribution of the westernmost subpopulation of Assamese macaques (Macaca assamensis pelops) under climate change: conservation implications of a threatened population

Anthropogenic climate change can have a negative impact on the distribution of habitat specialist species with narrow distributional ranges. Among the many areas around the world affected by climate change, the Himalayan region in particular is experiencing marked effects of global warming on its climatic patterns and life forms. This study assessed the current distribution of Assamese macaques (Macaca assamensis) in western Nepal, and projected their likely distribution (via habitat suitability modeling) for the years 2050 and 2070 under the two most plausible greenhouse gas emission scenarios (RCP4.5 and RCP6.0). We recorded a total of 159 Assamese macaques belonging to 10 groups (nine of them residing outside of protected area networks) between 684m and 2550m asl in western Nepal. Climatic niche modeling predicted that 37.03% (25,114 km2) of the total area of western Nepal (67,813 km2) represented suitable habitat of the species, the majority of which ranged between 1000 m and 2000 m asl. Future projections of the current climatic niche of the Assamese macaques revealed a consistent decrease in the suitable habitat for 2050 and 2070 in both of the greenhouse emission scenarios tested. With the RCP6.0 scenario, almost 57% of the current highly suitable habitat would be lost by 2070 with remaining suitable habitat shifted towards higher elevations. The loss of habitat directly affects the species survival and the range shift towards higher elevation exposes them to new areas for conflict with humans. Therefore, the Assamese macaque population in western Nepal requires a high conservation priority with the establishment of new protected areas.

The long-term gut bacterial signature of a wild primate is associated with a timing effect of pre- and postnatal maternal glucocorticoid levels

BackgroundDuring development, elevated levels of maternal glucocorticoids (GCs) can have detrimental effects on offspring morphology, cognition, and behavior as well as physiology and metabolism. Depending on the timing of exposure, such effects may vary in strength or even reverse in direction, may alleviate with age, or may concern more stable and long-term programming of phenotypic traits. Maternal effects on gut bacterial diversity, composition, and function, and the persistence of such effects into adulthood of long-lived model species in the natural habitats remain underexplored.ResultsIn a cross-sectional sample of infant, juvenile, and adult Assamese macaques, the timing of exposure to elevated maternal GCs during ontogeny was associated with the gut bacterial community of the offspring. Specifically, naturally varying maternal GC levels during early but not late gestation or lactation were associated with reduced bacterial richness. The overall effect of maternal GCs during early gestation on the gut bacterial composition and function exacerbated with offspring age and was 10 times stronger than the effect associated with exposure during late prenatal or postnatal periods. Instead, variation in maternal GCs during the late prenatal or postnatal period had less pronounced or less stable statistical effects and therefore a weaker effect on the entire bacterial community composition, particularly in adult individuals. Finally, higher early prenatal GCs were associated with an increase in the relative abundance of several potential pro-inflammatory bacteria and a decrease in the abundance of Bifidobacterium and other anti-inflammatory taxa, an effect that exacerbated with age.ConclusionsIn primates, the gut microbiota can be shaped by developmental effects with strong timing effects on plasticity and potentially detrimental consequences for adult health. Together with results on other macaque species, this study suggests potential detrimental developmental effects similar to rapid inflammaging, suggesting that prenatal exposure to high maternal GC concentrations is a common cause underlying both phenomena. Our findings await confirmation by metagenomic functional and causal analyses and by longitudinal studies of long-lived, ecologically flexible primates in their natural habitat, including developmental effects that originate before birth.3d25Wu_1Je2mxjb-DcHdXZVideo

Developmental and environmental modulation of fecal thyroid hormone levels in wild Assamese macaques (Macaca assamensis).

Thyroid hormones are key modulators of development, as well as mediators of environmental conditions, by regulating developmental processes and metabolism in primates. Hormone measurement in noninvasively collected samples, that is, feces and urine, is a valuable tool for studying the endocrine function of wildlife, and recent studies have demonstrated the feasibility of measuring thyroid hormones in fecal samples of zoo-housed and wild nonhuman primates. Our study aimed to (i) validate the measurement of immunoreactive fecal total triiodothyronine (IF-T3) in wild Assamese macaques (Macaca assamensis) and (ii) to investigate its developmental changes and its response to environmental changes, including stress responses, in immature individuals. Fecal samples and environmental parameters were collected from individuals of three social groups of wild Assamese macaques living at Phu Khieo Wildlife Sanctuary, Northeastern Thailand. Our study confirmed the methodological feasibility and biological validity of measuring IF-T3 in this population. Specifically, the biological validation demonstrated higher IF-T3 levels in immatures compared to adults, and higher levels in females during late gestation compared to the preconception stage. Our analysis of IF-T3 levels in developing immature macaques revealed a significant increase with age. Furthermore, we found a positive association between IF-T3 and immunoreactive fecal glucocorticoid levels, an indicator of the physiological stress response. Neither minimum temperature nor fruit abundance predicted variation in IF-T3 levels in the immatures. Our findings indicate the possibility for differing effects of climatic factors and food availability on thyroid hormone level changes in immature versus adult animals and in wild compared to experimental conditions. Overall, our study provides the basis for further investigations into the role of thyroid hormones in shaping species-specific traits, growth, and overall primate development.

UAV-assisted counts of group size facilitate accurate population surveys of the Critically Endangered cao vit gibbon Nomascus nasutus

AbstractGibbons are often difficult to observe in dense forest habitats using traditional ground-based methods. This makes it challenging to estimate group sizes and, in turn, population sizes. This has proven to be a key constraint on accurate monitoring of the last remaining population of the Critically Endangered cao vit gibbon Nomascus nasutus. However, new technologies are beginning to circumvent the problems associated with traditional methods. We hypothesized that, by using an unoccupied aerial vehicle (UAV) equipped with thermal and standard (RGB) cameras, we could obtain more accurate group size counts than by using ground-based observations, as fewer gibbons would be missed. We tested this during a population survey of the cao vit gibbon, finding that the thermal video footage revealed additional individuals that were not counted by ground-based surveyors. Statistically, there was strong evidence (93% probability) that UAV-derived counts were higher (by 41%) than concurrent ground-based counts. We recorded six primate groups of three species (cao vit gibbon, rhesus macaque Macaca mulatta and Assamese macaque Macaca assamensis), including 24 gibbons across four groups (c. 20% of the global population). The RGB video footage also revealed seven female gibbons, two of which were carrying infants, providing vital group composition data. These data have contributed directly to a more accurate population survey of the species than would have been possible using direct observation only. We anticipate more widespread use of UAVs in the study of gibbons and other threatened species, leading to a more robust evidence base for their conservation.

The Japan Monkey Centre Primates Brain Imaging Repository of high-resolution postmortem magnetic resonance imaging: The second phase of the archive of digital records

A comparison of neuroanatomical features of the brain between humans and our evolutionary relatives, nonhuman primates, is key to understanding the human brain system and the neural basis of mental and neurological disorders. Although most comparative MRI studies of human and nonhuman primate brains have been based on brains of primates that had been used as subjects in experiments, it is essential to investigate various species of nonhuman primates in order to elucidate and interpret the diversity of neuroanatomy features among humans and nonhuman primates. To develop a research platform for this purpose, it is necessary to harmonize the scientific contributions of studies with the standards of animal ethics, animal welfare, and the conservation of brain information for long-term continuation of the field. In previous research, we first developed a gated data-repository of anatomical images obtained using 9.4-T ex vivo MRI of postmortem brain samples from 12 nonhuman primate species, and which are stored at the Japan Monkey Centre. In the present study, as a second phase, we released a collection of T2-weighted images and diffusion tensor images obtained in nine species: white-throated capuchin, Bolivian squirrel monkey, stump-tailed macaque, Tibet monkey, Sykes’ monkey, Assamese macaque, pig-tailed macaque, crested macaque, and chimpanzee. Our image repository should facilitate scientific discoveries in the field of comparative neuroscience. This repository can also promote animal ethics and animal welfare in experiments with nonhuman primate models by optimizing methods for in vivo and ex vivo MRI scanning of brains and supporting veterinary neuroradiological education. In addition, the repository is expected to contribute to conservation, preserving information about the brains of various primates, including endangered species, in a permanent digital form.

Impact of Altitude on Population Structure and Distribution of Assamese Macaque (Macaca assamensis Mc’clelland, 1840) in Dampa Tiger Reserve in Mizoram, India

A study on population status and distribution of Assamese macaque ( Macaca assamensis ) across altitudinal gradients in Dampa Tiger Reserve (DTR), Mizoram, India was conducted. Six species of diurnal primates inhabits in DTR and a study on Assamese macaque is reported here. Line transact method was adopted for population sampling. Total distance covered was 293.5 km. Data on group locations, demography and site covariates including trail length, duration of walk, forest type were recorded. Nine groups of Assamese macaque comprising of 123 individuals were recorded mostly at an altitude between 250 m to 856 m. The smallest group was consisted of 7 individuals, and the largest was of 21 individuals. An average group size calculated was 13.6 (±4.32) individuals. The population comprised of 36.59% male, 53.66% female, and 9.76% immature. All the groups were comprised of multi-males and multi-females. The population encounter rate was 0.42 individual/km and the group encounter rate was 0.03group/ km. Total male to female ratio was 1:1.46, adult adult male to female ratio was 1:1.53. All female to immature ratio was 1:0.18 and an adult female to immature ratio was 1:0.26. Maximum sighting were at the distance between 30-50 m. Area of occupancy of groups was about 26 km 2 and ecological density was 4.7/ km 2 . Among primates in DTR, wild population of Assamese macaques were found to occupy altitudinal range between 250 to 856 m. 67% individuals were distributed at the altitude between 400 to 600 m. Presence of other primate species in DTR restricted the occurrence of this species.

Impact of Altitude on Population Structure and Distribution of Assamese Macaque (Macaca assamensis Mc’clelland, 1840) in Dampa Tiger Reserve in Mizoram, India

A study on population status and distribution of Assamese macaque ( Macaca assamensis ) across altitudinal gradients in Dampa Tiger Reserve (DTR), Mizoram, India was conducted. Six species of diurnal primates inhabits in DTR and a study on Assamese macaque is reported here. Line transact method was adopted for population sampling. Total distance covered was 293.5 km. Data on group locations, demography and site covariates including trail length, duration of walk, forest type were recorded. Nine groups of Assamese macaque comprising of 123 individuals were recorded mostly at an altitude between 250 m to 856 m. The smallest group was consisted of 7 individuals, and the largest was of 21 individuals. An average group size calculated was 13.6 (±4.32) individuals. The population comprised of 36.59% male, 53.66% female, and 9.76% immature. All the groups were comprised of multi-males and multi-females. The population encounter rate was 0.42 individual/km and the group encounter rate was 0.03group/ km. Total male to female ratio was 1:1.46, adult adult male to female ratio was 1:1.53. All female to immature ratio was 1:0.18 and an adult female to immature ratio was 1:0.26. Maximum sighting were at the distance between 30-50 m. Area of occupancy of groups was about 26 km 2 and ecological density was 4.7/ km 2 . Among primates in DTR, wild population of Assamese macaques were found to occupy altitudinal range between 250 to 856 m. 67% individuals were distributed at the altitude between 400 to 600 m. Presence of other primate species in DTR restricted the occurrence of this species.

Dietary preference of Assamese Macaque Macaca assamensis McClelland, 1840 (Mammalia: Primates: Cercopithecidae) in Dampa Tiger Reserve, India

Dietary composition and selection of food items are important approaches for the flexibility and adaptability of macaques in different natural habitats. With a wide distribution range, Assamese Macaques feed on various food types. This study reports the consumption of 57 plant species from 30 families. A total of 2,233 scans resulted in 16,381 feeding behavioral records during the study period from 2018 to 2020. Macaques appear to be primarily folivorous in Dampa as leaves (young & mature) constitute 44.74% of their daily dietary intake while the fruit consumption was found to be 25.31% of the total dietary intake. Plant species like Artocarpus lakoocha (15.65%), Albizia procera (12.03%), Glochidion hyneanum (10.53%), Diospyros glandulosa (9.49%), and Albizia lebbeck (7.28%) contributed significantly to macaque’s diet compare to other plants. No significant variation was observed on time spent for feeding on leaves, fruits, flowers, and seeds in both different months and seasons of the year. The highest percentage of the diurnal time invested on feeding activity was (59.04%) in the month of January (winter season), which may be due to the cold climate and scarcity of proper feeding items and the least was (35.19%) in June where food resources are more readily available. The richness of fruiting plants in Dampa Tiger Reserve appears to fulfill the dietary requirement of Assamese Macaque and therefore intactness of forest resources is necessary for their development and conservation.

Growth trajectories of wild Assamese macaques (Macaca assamensis) determined from parallel laser photogrammetry

Socioecological factors are associated with life-history patterns and growth trajectories among primates. Under certain conditions, selection may favor a temporal decoupling of growth and major life-history events such as sexual maturation or natal dispersal. Yet, empirical tests of these associations in wild populations remain scarce owing to the lack of non-invasive methods to capture growth trajectories. In this study, we first compared two non-invasive methods of digital photogrammetry. Then, we used parallel laser photogrammetry to investigate forearm growth of wild Assamese macaque males and females in their natural habitat at Phu Khieo Wildlife Sanctuary, Thailand to test life-history and socio-ecological hypotheses. Across 48 males and 44 females, we estimated growth trajectories and pseudo-velocity curves by applying quadratic plateau models and non-parametric LOESS regressions. We assessed the development of sexual dimorphism by comparing the sexes at five different ages. Females had completed 96% of their growth at the age at first birth (5.9 years) and ceased growing at 7.1 years of age. Males, in contrast, grew until well after their average age of natal dispersal: they reached 81% of their size at the age of natal dispersal (4.0 years), and ceased growing only at 9.0 years of age, much later than females. Sexual dimorphism in forearm length was driven by an extended growth period in males, which is expected for males dispersing between multimale and multifemale groups and not facing the risk of being ousted by other larger males. Our results contradict the neonatal investment hypothesis that predicts a desynchronization of investment in growth and reproduction only in female baboons, but not other papionins producing cheaper neonates. Furthermore, male Assamese macaques do not delay natal dispersal until they are fully grown, in accordance with predictions of the male-career-framework for species with low to medium level of direct competition.

Aging gut microbiota of wild macaques are equally diverse, less stable, but progressively personalized

BackgroundPronounced heterogeneity of age trajectories has been identified as a hallmark of the gut microbiota in humans and has been explained by marked changes in lifestyle and health condition. Comparatively, age-related personalization of microbiota is understudied in natural systems limiting our comprehension of patterns observed in humans from ecological and evolutionary perspectives.ResultsHere, we tested age-related changes in the diversity, stability, and composition of the gut bacterial community using 16S rRNA gene sequencing with dense repeated sampling over three seasons in a cross-sectional age sample of adult female Assamese macaques (Macaca assamensis) living in their natural forest habitat. Gut bacterial composition exhibited a personal signature which became less stable as individuals aged. This lack of stability was not explained by differences in microbiota diversity but rather linked to an increase in the relative abundance of rare bacterial taxa. The lack of age-related changes in core taxa or convergence with age to a common state of the community hampered predicting gut bacterial composition of aged individuals. On the contrary, we found increasing personalization of the gut bacterial composition with age, indicating that composition in older individuals was increasingly divergent from the rest of the population. Reduced direct transmission of bacteria resulting from decreasing social activity may contribute to, but not be sufficient to explain, increasing personalization with age.ConclusionsTogether, our results challenge the assumption of a constant microbiota through adult life in a wild primate. Within the limits of this study, the fact that increasing personalization of the aging microbiota is not restricted to humans suggests the underlying process to be evolved instead of provoked only by modern lifestyle of and health care for the elderly.8UGmGK4ynviQrPL4KJ5rpeVideo abstract

Depredation of Crop Caused By Monkeys nearby ShivapuriNagarjun National Park, Kathmandu, Nepal

Macaca mulatta, and Macaca assamensis are common monkeys in Nepal. The Macaca assamensis was categorized as ‘Vulnerable’ under IUCN Red List. The habitat of the monkey was disturbed because of human interference and hence the consequence is frequent damage of human’s properties. However, there was limited study regarding the crop damage caused by monkeys in Nepal. Therefore, this study was objectively conducted to assess the damages and their spatial distribution caused by Monkeys nearby Shivapuri-Nagarjun National Park and also find the causes of interface between humans and Monkey. Four sites were visited and 120 house hold survey was done to collect the primary data. The GPS coordinates of the spatial distribution of damages were recorded. Secondary information about the damages was collected from 2019 to 2022. Descriptive and statistical analysis like trend analysis, principle component analysis and mapping of distribution of damages was done. The result showed that, the highest damage of the maize at Nayagaun, Thulogaun with 650 Kg while this was the lowest around 280 Kg. Similarly, the highest maize damage at Naagarjun, Gufagairi, Sarung danda was 400 Kg while the highest record of crop damage at Raniban, Ichangu Narayan was 190 Kg. The distribution map showed that there were 12 locations of maize damage over 450 kg at Nayagaun, Thulogaun. Similarly, the map of Goldhunga showed 17 places having medium damage (120-350 kg), 28 places of low damage (50-120 kg) while the 5 places of very low damage (<50 kg). The principle component analysis showed the causes of interface between humans and monkeys. The use of catapult in Nayagaun, Thulogaun, shout and charge threat in Naagarjun, Gufagairi, Sarung danda and in Raniban, Ichangu Narayan were the most influencing factors of this interface. This research will be useful for scientific community and policy maker.

Pathways to paternal care in primates.

Natural selection will favor male care when males have limited alternative mating opportunities, can invest in their own offspring, and when care enhances males' fitness. These conditions are easiest to fulfill in pair-bonded species, but neither male care nor stable "breeding bonds" that facilitate it are limited to pair-bonded species. We review evidence of paternal care and extended breeding bonds in owl monkeys, baboons, Assamese macaques, mountain gorillas, and chimpanzees. The data, which span social/mating systems and ecologies, suggest that there are multiple pathways by which conditions conducive to male care can arise. This diversity highlights the difficulty of making inferences about the emergence of male care in early hominins based on single traits visible in the fossil record. We discuss what types of data are most needed and the questions yet to be answered about the evolution of male care and extended breeding bonds in the primate order.