Extract Of Asparagus Racemosus Research Articles

Multi Receptor Targeting of Potential Bioactive Compound Obtained from Chloroform Extract of Asparagus racemosus by GC-MS Analysis: A in silico Based Approach

Multi Receptor Targeting of Potential Bioactive Compound Obtained from Chloroform Extract of Asparagus racemosus by GC-MS Analysis: A in silico Based Approach

In-vitro Evaluation for Anticancer Activity of Extract of Asparagus racemosus

The main purpose of this study is to look into how protective Asparagus racemosus leaves are in-vitro. Cancer is a major reason people die around the world. Many of the bad effects of the allopathic drugs that are now used to treat cancer have caused people to turn to plant medicine. Some of the solvents that were used in the sequential solvent extraction method were chloroform, ethanol and ethyl acetate. The chloroform extract had a higher percentage yield compared to the other extracts. Because it had anticancer activity in-vitro, the therapeutically active extract was picked to have anticancer activity in living things. The MTT test was used to see if the chloroform, ethyl acetate, and ethanol extracts could help fight cancer in HeLa cell types. The MTT assay showed that chloroform and ethyl acetate extracts had strong anticancer activity. These two extracts were chosen for study into how they work against cancer in living things

Biosynthesis of Copper Nanoparticles with an Ethanolic Asparagus racemosus Root Extract: Environmental Protection Against Blue-Green Algae and its Bactericidal Potential

This study was done to prove the environmental application of copper nanoparticles, particularly theantimicrobial activity against bacterial pathogens, Pseudomonas aeruginosa and Staphylococcus aureus. The root extract ofAsparagus racemosus was used to synthesize copper nanoparticles (Cu-NPs) via biosynthesis. Cu-NPs were characterizedusing a UV-Visible spectrophotometer, X-ray diffraction, Fourier transform infrared spectroscopy, and scanning electronmicroscopy. The antibacterial activity of Cu-NPs was studied by treating nanoparticles with two bacterial pathogens,Pseudomonas aeruginosa and Staphylococcus aureus. The biosynthesized copper nanoparticles displayed satisfactoryantibacterial activity against bacterial pathogens. Antioxidant and antidiabetic assays were performed via the enzymeinhibition property. The biosynthesized Cu-NPs exhibited a satisfactory antifouling function to eradicate algae growth.

In silico Based Virtual Screening of Non-polar Phytochemicals obtained From Petroleum Ether Extract of Asparagus racemosus by GC-MS Analysis

In silico Based Virtual Screening of Non-polar Phytochemicals obtained From Petroleum Ether Extract of Asparagus racemosus by GC-MS Analysis

Role of Aqueous and Ethanolic Seed Extract of Asparagus racemosus on Acr-Induced Neurotoxicity in Adult Zebrafish: Emergence of Neuroprotective Results

Role of Aqueous and Ethanolic Seed Extract of Asparagus racemosus on Acr-Induced Neurotoxicity in Adult Zebrafish: Emergence of Neuroprotective Results

Green Synthesis of TiO2 Nanoparticles Using Root Extract of Asparagus racemosus (Shatavari) and Evaluation of its Antibacterial, Antioxidant and Antidiabetic activities

Since ancient times, Asparagus racemosus, a traditional herb with various health advantages, has been used to cure a wide range of illnesses. Researchers sparked a lot of interest in the biological methods for producing nanoparticles (NPs). This study’s objective was to synthesize titanium dioxide nanoparticles (TiO2 NPs) utilizing the top-down nanofabrication method. Asparagus racemosus root extract was used to produce titanium nanoparticles utilizing green chemistry. The nanoparticles were characterized using UV-Visible spectroscopy, SEM (Scanning Electron Microscope), and EDX. According to the SEM images the particles are spherical in shape with an average size of 89.59 nm. The antibacterial activity was done with 3 bacterial strains i.e., E. coli, Pseudomonas aeruginosa and Bacillus subtills. Antioxidant activity was evaluated by DPPH and phosphomolybdate assays, the calculated IC50 was 85.18±0.55 µg/ml and 66.4±1.3 µg/ml respectively. The calculated IC50 value for alpha amylase assay was 46.59±2.81 µg/ml. As per the outcomes it is concluded that the plant mediated titanium nanoparticle is very potent which can be used in pharmaceutical and biomedical areas.

Implications of Asparagus racemosus and Terminalia chebula extracts on oxazolone induced inflammatory bowel disease in Danio rerio (zebrafish)

Inflammatory Bowel Disease (IBD) occurs in the human intestine without any definitive etiology for which there is no cure yet. The present study analyses the anti-inflammatory and anti Inflammatory Bowel Disease (IBD) potentials of the methanol extract of Indian herbal plants Asparagus racemosus (AR) and Terminalia chebula (TC). First, we prepared methanolic extracts of AR root and TC fruit using the Soxhlet procedure and analyzed their phytochemical composition by GC-MS. Second, the extracts were used against Escherichia coli and Bacillus cereus cultures and observed for growth and biofilm inhibition. Third, the anti-oxidant assays were performed for the same extracts. Finally, we studied pathological and hematological analysis to study a reduction in the number of inflammatory cells following oxazolone-induced IBD in the zebrafish (Danio rerio). Both extracts contain steroids, terpenoids, tannins, phenols, and carbohydrates as active ingredients with moderate antibacterial activity against Escherichia coli and Bacillus cereus with minimum inhibitory concentration (MIC) of 12.5 μg/ml (TC) and 25 μg/ml (AR) against both bacteria. AR extract showed high biofilm inhibition on E. coli (80.8 ± 0.81%) and B. cereus (92.8 ± 1.08%) at 800 μg/mL and showed high DPPH radical scavenging activity IC50 value of 139.9 μg/mL. Further in IBD zebrafish, AR (14 mg/L) and TC (60 mg/L) exhibited a significant reduction in inflammatory cells in blood and improved pathological scores in the gut compared with positive drug Prednisolone (50 mg/L). Moreover, T. chebula showed a greater reduction in the number of inflammatory cells recruited in the intestine of IBD-induced fish compared to A. racemosus at the time point and dose examined.

Formulation and Evaluation of Niosomal Gel Loaded with Asparagus racemosus Extract for Anti-inflammatory Activity

Abstract: Background/Aim: Inflammation typically occurs when infectious microorganisms enter the body, settle in specific tissues, and/or circulate in the blood. Asparagus racemosus extract contains various saponins and flavonoids and plant-origin drugs have fewer side effects and toxicity. The phytoconstituents have less permeability through the skin; to enhance their permeability and effectiveness it was loaded in niosomes. Therefore, the study aimed to formulate and characterize the niosomal gel loaded with Asparagus racemosus extract for anti-inflammatory activity. Materials and Methods: The niosomes containing saponins in the extract were prepared using thin film hydration method and 23 full factorial design was employed to assess the influence of independent variables span 60 and cholesterol on vesicle size, PDI, zeta potential, and percentage entrapment efficiency. 10% of niosomal and conventional gels were prepared by incorporating optimized niosomes and extract containing total saponin in 1% carbopol gel. Ex vivo permeability studies of prepared gels were performed through goat skin using Franz diffusion cell. An anti-inflammatory study was conducted on albino rat. Results: The statistical analysis revealed the significant effect of independent variables on vesicle size, PDI, entrapment efficiency and zeta potential. SEM image shows vesicles are spherical in shape and uniform in size. The niosomal gel provided a significantly higher amount of steady-state flux and permeability coefficient into the skin than conventional gel. The animal model proved that niosomal gel loaded with total saponins in extract showed significant anti-inflammatory compared to the control. Conclusion: It was concluded that the niosomal gel had better efficacy than the conventional gel. Keywords: Asparagus racemosus, Niosomes, Span 60, Cholesterol, Factorial design, Carbopol 934.

Microstructural evidence of reversal of PCOS by steroidal saponins of asparagus racemosus in PCOS induced rats

The purpose of this study is to investigate the histological and hormonal observations in fructose-fed, letrozole-induced polycysticovarian- syndrome (PCOS) rats treated with various doses of extract of asparagus racemosus (EAR) and Steroidal saponin (SAPO). 48 female Wistar albino rats were divided into 8 groups, including Vehicle Control (VC); PCOS; EAR 400 mg/kg; SAPO 40 mg/kg; PCOS + EAR 200 mg/kg; PCOS + EAR 400 mg/kg; PCOS + SAPO 20 mg/ kg; PCOS + SAPO 40 mg/kg. PCOS group was administered letrozole at a concentration of 1 mg/kg dissolved in 1% CMC per oral(p.o.) once daily for 28 days. Along with these, rats were allowed free access of 10% fructose solution daily. Calculated dosages of EAR and SAPO were given with oral gavage for 30 days. During experimental period, vaginal smears were collected daily for estrus cycle determination. Rats were sacrificed and blood samples were collected for hormonal assay. Ovaries were removed to proceed with histopathological study. Slides were stained using hematoxylin and eosin (H&E) stains. When compared to the vehicle control group, PCOS ovaries had a higher incidence of ovarian cysts, incomplete luteinization, and a lower number of corpus lutea. Although serum estradiol, progesterone, and Follicle-stimulating hormone (FSH) levels were lower in the PCOS group, testosterone and luteinizing hormone (LH) levels were higher. The findings of this study indicated that taking EAR 400 mg/kg and SAPO 40mg/kg orally could alleviate PCOS-related symptoms. It appears that consuming SAPO 40mg/kg reduces LH and testosterone levels while increasing FSH, estrogen, and progesterone hormone levels. Because of the hormonal balancing nature of these drugs, EAR 400mg/kg- and SAPO 40mg/ kg-treated rats had a lower number of cystic follicles and a higher number of corpora lutea. In PCOS rats, this results in a normal process of folliculogenesis and ovulation. In the current study, we observed that SAPO 40mg/kg is better compared to EAR 400mg/kg treatment.

Asparagus racemosus improves immune-related parameters in Nile tilapia (Oreochromis niloticus) and mitigates deltamethrin-induced toxicity

Deltamethrin (DM) is one of the most toxic but widely used pyrethroid insecticides. Even though a non-target animal, fish are at high risk as they are deficient in the enzyme system that hydrolyses pyrethroids. Enhancing the immune system is a potential method in preventing fish diseases. The present investigation aims to study the modulations in the immune response-related parameters in Oreochromis niloticus that were exposed to DM, by dietary supplementation of aqueous root extract of Asparagus racemosus (ARE). The experiment compared fish in control, DM (1 μg/L) exposed (added to water), ARE (10 g, 20 g, and 30 g ARE/kg of feed) supplemented, and DM-ARE cotreated groups. After 21 days of experimental period, serological, histopathological, and immune response related-gene and protein analysis were carried out. The DM-ARE cotreated group showed significant increase in weight gain, specific growth rate, and decreased feed conversion ratio compared to the DM exposed group. The ARE cotreatment could significantly revert the alteration induced by DM in lysozyme, respiratory burst, myeloperoxidase, C-reactive protein, glucose, cortisol, total protein, albumin, and triglyceride levels. The liver histopathology showed membrane breakage, severe necrosis, infiltration of inflammatory cells, melano-macrophages, and nuclear atrophy, and the kidney showed tubular necrosis, hematopoietic necrosis, Bowman's capsule edema, and glomerulus degeneration in DM exposed group. In ARE cotreated group, the liver showed regenerative cellular changes and only mild to moderate cellular damages, and the kidney tubules and glomerulus had intact structure. ARE discernibly regulated the expression of immune-related genes and proteins (IgM, TNFα, IFN-γ, IL-1β, and IL-8) in fish. The DM-ARE cotreated groups showed reduced cumulative mortality and higher relative percent survival on experimental challenge with Aeromonas hydrophila compared to the DM group. Thus, ARE possess protective potential against DM-induced toxicity, and can be used as a cost-effective technique in aquafarming.

Antibacterial activity and cytotoxic effect of bisphosphonate conjugated gold nanoparticle synthesized using asparagus racemosus root extract

Nanoparticles are being widely used in the field of medicine called Nanomedicine. Biological membranes in the human body are selectively permeable to Nanoparticles. The nanoparticles can be surface modified to attach a variety of ligands which enable them to be biosensors, molecular-scale fluorescent tags, imaging agents, and targeted drug delivery vehicles. Green synthesis of nanoparticles is a type of bottom-up approach in which reaction occurs with the help of reducing and stabilizing agents. Bisphosphonates are increasingly being used to treat a wide range of skeletal issues, including hereditary skeletal disorders in children, cancer-related bone metastases and osteoporosis. Asparagus racemosus is a green edible medical plant used in Ayurvedic preparations to treat aging, vigor, immunity, longevity, and skeletal issues. It has been shown to have a variety of pharmacological properties, including antioxidant, antimicrobial, anti-inflammatory, and antiseptic properties. Bisphosphonate conjugated with gold nanoparticle have shown to produce more targeted action.The goal of this study was to green synthesise Bisphosphonate conjugated gold nanoparticles with asparagus racemosus extract and to check their antimicrobial activity and cytotoxicity. Green synthesis of Bisphosphonate conjugated gold nanoparticles using Asparagus racemosus extract was achieved and is characterized.

Evaluation of Shatavarin IV Compound from Methanolic Extract of Asparagus racemosus by High Performance Thin Layer Chromatography

Asparagus racemosus is an herb of Asparagus or Liliaceae family. It is commonly utilized in human and animal husbandry care management. Asparagus racemosus has powerful antioxidant anti-inflammatory uterine tonic antimicrobial, anti-cancer, and galactagogue properties. Shatavarin is a steroidal saponin found in Asparagus racemosus roots. In this experiment, roots of Asparagus racemosus were collected from Herbal Garden, Ethnos Veterinary Herbal Product Research and Development Centre, Tamil Nadu Veterinary and Animal Sciences University, Orathanadu, Thanjavur District, Tamil Nadu. Shatavarin IV was identified and quantified by a simple precise and reliable High Performance Thin Layer Chromatography technique.

Green synthesis and characterization of bisphosphonate conjugated gold nanoparticle with Asparagus racemosus root extract.

Bisphosphonates improve orthodontic anchorage. More targeted action of this drug can be achieved through its conjugation with gold nanoparticles. Asparagus racemosus is a green edible medicinal plant used in Ayurvedic preparations to treat aging, vigor, immunity, longevity, and skeletal issues. Therefore, it is of interest to report the green synthesized Bisphosphonate conjugated gold nanoparticles with Asparagus racemosus extract and to characterize them.

Bactericidal action of copper nanoparticles synthesized from methanolic root extract of Asparagus racemosus

The main aim of the study is to exhibit an antibacterial activity using copper nanoparticles against two bacterial pathogens such as Escherichia coli, and Staphylococcus aureus. Initially, Copper nanoparticles were synthesized by using the Asparagus racemosus root extract and the centrifuged copper nanoparticles were dried in normal room temperature for the future use. Then Ultra Violet analysis, X-ray Diffraction method, Fourier Transform Infrared Spectroscopy and Zeta potential were used to characterize for the confirmation of copper nanoparticles. The antibacterial activities were experimentally studied using two pathogens (Escherichia coli, and Staphylococcus aureus) and this result shows a very good antibacterial activity in biosynthesized copper nanoparticles.

Modulation of steroid hormone synthesis by alcoholic extract of Asparagus racemosus on MCF-7 cells

The objective of the study was to elucidate the effect of alcoholic extract of A. racemosus on oestrogen and progesterone synthesis from MCF-7 cells. The root tubers of A. racemosus was collected dried, extracted in soxhlet, solvent evaporated and dried and was used for study. Qualitative phytochemical analysis of the extract was done. The IC50 was determined by doing MTT assay in MCF-7 cell lines. MCF-7 cells cultured in RPMI-1640 were exposed to IC50, half and double the doses of IC50 for 96 hours and the media collected every 48 hours to determine the concentration of oestrogen and progesterone using ELISA. The qualitative phytochemical analysis of the extract revealed the presence of steroids, alkaloids, diterpenes, triterpenes, tannins, glycosides and saponins The IC50 was found to be 267 µg/mL. The extract of A. racemosus caused an increase in the concentration of oestrogen and progesterone secreted by MCf-7 cells in a dose and time dependent fashion. There was an increase in the secretion of progesterone in a dose depenedent fashion compared to untreated cells, whereas the secretion decreased at 96 hours compared to 48 hours. From the study, it could be concluded that alcoholic extract of A. racemosus caused a positive modulation of steroid hormone synthesis.

Combined Effect of Asparagus Racemosus and Ecliptaalba as Anticandidal Drug and its Underlying Pharmacognostic Properties

racemosus is widely used in Ayurveda to cure various ailments. Plants and plant derived preparations have been used as traditional remedies and in medicines for the different treatments. The in vitroanticandidalactivity of Shatavri the “queen of herbs” and Eclipta alba “False daisy’’ and “King of hairs” was tested against Candida Albicans and various extract of both the plants were evaluated for Pharmacognostic studies. Ecliptaalba is an important medicinal plant used in treatment of various health problems including digestion, asthma, cough, headache, and skin color.It also shows antimicrobial activity against candida and a very promising result against the fungal strain. Also the extract of Asparagus racemosus showed high degree of activity in case the same strain and both the plants were found similar with that of standardantibiotics use. This research paper throws light on the above pharmacognostic and antimicrobial properties. Both the plants were used as therapeutic agent for human disease.

Neuromodulatory potential of Asparagus racemosus and its bioactive molecule Shatavarin IV by enhancing synaptic acetylcholine level and nAChR activity

Cholinergic dysfunction has been commonly known to be associated with plethora of neurodegenerative disorders and also serves as a biomarker. Recently, cholinergic system demonstrated that acetylcholine has major role in regulation of its function therefore the main therapeutic regimens towards disease management have been focused on increasing acetylcholine levels. The current study explores the potential of Asparagus racemosus extract (ARE) and its bioactive molecule Shatavarin IV (SIV) in improving cholinergic transmission via utilizing Caenorhabditis elegans considering as a model system. Observations and results obtained through this study have clearly showed significant modulation in cholinergic function by increasing acetylcholine (ACh) levels and the nicotinic acetylcholine receptors (nAChRs) activity. Further exploration on mechanistic facet pointed towards ARE and SIV modulatory potential through increased synaptic ACh level by blocking acetyl cholinesterase at enzyme level and by regulating increment in transcript level of cha-1, and cho-1 that are directly responsible for the synthesis of ACh. Further, the up-regulation of unc-38 and unc-50 transcripts could be the reason for enhanced nAChR activity and investigation on stress modulator activity showed excellent efficiency of ARE and SIV in diminishing ROS thereby lowering the oxidative damage.

Neuroprotective Efficacy of Asparagus racemosus Root Extract on Hippocampal Neurons in Scopolamine Mouse Model of Alzheimer’s Disease

Background: Alzheimer’s disease is a one of the prominent cause of senile dementia in the aged people, resulting in mental health deterioration in more than 44 millions of people worldwide. A great majority of currently available medication for neurodegenerative disorders like AD are synthetic in origin and the most of them cause severe side effects. In Ayurveda, an Indian traditional medicinal system, natural products of plant origin has used in since time immorial for treatment of neurodegenerative disorders with the minimum side effects. Asparagus racemosus a household plant is one of them known to possess various biological properties such as antioxidant, anti-apoptotic, adaptogen and Ayuvedic practitioners for neuronal disorders have used for it traditionally. In previous published study from this laboratory, we have shown the nootropic properties of methanolic root extract of Asparagus racemosus using behavioural paradigm. Asparagus racemosus have phytoestrogenic properties with mild estrogenic activities. Estrogen has been reported as potent neuroprotective in various in vivo and clinical studies. In this consequence, the aim of this study was to examine neurorprotective effects of Asparagus racemosus root extraction hippocampal neurons in scopolamine induced Alzheimer’s type dementia in mice. Materials and Methods: The roots of Asparagus racemosus were powdered and extracted in Soxhlet apparatus. The dose of methanolic extract of AR was evaluated for its neuroprotective efficacy against scopolamine induced amnesia in mice. Results and Conclusion: The results of histopathological evaluation showed that methanolic root extract of Asparagus racemosus attenuated the neuronal death in hippocampal CA1, CA3 and DG region induced by scopolamine. These findings indicate that phytoestrogen may serve as potential preventive and therapeutic agent for neurodegenerative disorders.

PRELIMINARY PHYTOCHEMICAL SCREENING AND ANTIBACTERIAL ACTIVITY OF ASPARAGUS RACEMOSUS LEAF EXTRACT ON FOOD BORNE ENTEROBACTERIACEAE

Enterobacteria are often responsible for various gastrointestinal foods borne infection in humans. But due to profuse use of commercial antibiotic and synthetic pesticides for human and crop protection growing incidences of drug-resistant pathogens of both clinical and agricultural importance have increased. This has aggravated the search for antimicrobials from plants sources. Medicinal plants have intrinsic ability to resist pathogenic microorganisms and this has led the researchers to investigate and explore them for the treatment of food borne infections of humans by developing new antimicrobial agents. The aim of this study was to evaluate the antimicrobial properties of hydroalcoholic leaf extracts of Asparagus racemosus against foodborne bacterial isolates isolated from fresh raw vegetables. Primary qualitative analysis showed presence of different phytochemicals in the leaf extracts. The leaf extracts were screened in vitro in the laboratory for their antibacterial activity against the isolated enteric bacteria, and the results showed that hydroalcoholic extracts of Asparagus racemosus can be optimized clinically for chemotherapeutic control of these food-borne enteric infections. In conclusion, Asparagus racemosus extract exhibits antimicrobial activities and thus might be developed as natural sanitizer for washing raw vegetables.

Cytotoxic and antiproliferative potential of methanolic extracts of Asparagus racemosus in MDAMB231 cells

The present study was carried out to evaluate the cytotoxic and antiproliferative activity of methanolic extract of Asparagus racemosus in MDAB-231 cells. The qualitative phytochemical analysis of the extract revealed the presence of steroids, alkaloids, flavonoids, glycosides, saponins and diterpenes. MDAMB-231 cells were maintained in RPMI media containing 10 per cent serum and 1 per cent antibiotic and antimycotic solution. The cells were harvested and seeded to 96 well plate at 5×103 cells/mL, incubated for 24 hours at 37˚C with 5 per cent CO2. The cells were treated with 160, 80, 40, 20 and 10µg/mL of the extract for 24 hours and viability was accessed using MTT Assay. Also cells were plated in 6 well plates at concentrations 3×105 cells/mL and exposed to 80, 40, 20 and 10µg/mL of the extract of Asparagus racemosus for acridine orange ethidium bromide (AO/EB) staining. There was a dose dependent decrease in viability of cells exposed to methanolic extracts of Asparagus racemosus with a viability of 15.55+0.193 per cent for cells treated with 160µg/mL. The IC50 was found to be 91.36+ 0.87 µg/mL. The AO/EB staining revealed that most of the cells were dead in extract treated with 80µg/mL where more than 90 per cent of cells were live when treated with 10µg/mL of the extract.