The study aims to evaluate the behavioraland insecticidal effects ofRutaangustifoliaEO (RAEO) against larvae and adults of a major pest insectTribolium castaneum.RAEO exhibited a strong repellency against both adults and larvae where percentages as high as 74 and 72% of PR (Percentage of Repellency) were recorded respectively, at 0.47 µL/cm2 after 2h. Regarding the antifeedant bioassay, no feeding deterrence was recorded in adults while an increase in appetite was registered in larvae exhibiting -80% of FDI recorded at 0.2 µL/pellet. In terms of feeding preference, the response significantly depended on thedevelopmental stage(F=97.19, P<0.05) and larvae were attracted to treated pellets recording percentages of 84 and 73% of PI (Preference index) after 5 and 30 min at 0.05 µL/pellet; on the other hand, adults expressed a repulsive behavior at 0.2 µL/pellet with a PI of -81.1 (20min),and -69.2% (30 min). Regarding topical application toxicity, asignificant difference wasobserved between larvae and adults (p<0.05 F=174.56). The study promotes the use ofRAEO as a repellent andbioinsecticide for the control of larvae and adults of T. castaneum.