Ash Weight Research Articles

Effects of phytase and 25-hydroxycholecalciferol supplementation in broilers fed calcium-phosphorous deficient diets, with or without Eimeria challenge, on growth performance, body composition, bone development, and gut health

The study evaluated the effects of nutritional strategies on broilers challenged with Eimeria from days 14 to 26. A total of 840 Cobb male broilers were fed five diets in a 2 × 5 factorial arrangement: 1) nutrient adequate diet (PC; 0.84% calcium [Ca], 0.42% available phosphorus [avP]); 2) Ca-P deficient diet (NC; 0.64% Ca, 0.22% avP); 3) NC + 1500 FTU/kg phytase of diet (NC+PHY); 4) NC + 5000 IU/kg 25-hydroxycholecalciferol of diet (NC+25OHD); and 5) NC with both supplements (NC+PHY+25OHD), with and without Eimeria challenge. All treatments had six replicate cages with 14 birds per cage. At 5 days post inoculation (DPI), the challenged birds exhibited higher serum fluorescein isothiocyanate-d (FITC-d) levels than the unchallenged birds (P < 0.001). The NC+PHY and NC+PHY+25OHD groups exhibited lower FITC-d levels compared to the NC+25OHD group (P = 0.012). Significant interaction effects between Eimeria challenge and dietary treatments were observed on various parameters. During 0-6 and 0-12 DPI, Eimeria challenge resulted in decreased the body weight gain (BWG) (P < 0.05) but had a negative effect on the feed conversion ratio (FCR) in birds compared to the unchallenged group (P < 0.05). Reducing Ca and avP levels in the diet (NC) did not adversely affect BWG, but negatively impacted FCR, bone ash weight, ash concentration, and femur bone microstructure parameters (P < 0.05). On 12 DPI, Eimeria challenge led to decreased tibia bone weight, bone volume, fat free bone weight (FFBW), and ash weight of birds (P < 0.05). Supplementation with phytase alone or in combination with 25OHD improved growth performance, gut permeability, bone ash and bone microstructure parameters in birds (P < 0.05). However, the group fed 25OHD alone showed enhancements on growth performance, mineral apposition rate (MAR), bone ash concentration and ash percentage of the birds (P < 0.05). In conclusion, lowering Ca and avP levels in the diet negatively affected FCR and bone development but did not affect intestinal integrity in broilers. Dietary supplementation of phytase, 25OHD, or phytase in combination of 25OHD could enhance the growth performance and bone quality of broilers infected with Eimeria. Notably, the benefits of phytase supplementation were generally more pronounced than those associated with 25OHD supplementation; however, the combination of phytase and 25OHD could induce optimum effects.

Mix design determination procedure for geopolymer concrete based on target strength method

This study presents the development and validation of a mix design determination procedure for geopolymer concrete to achieve the desired compressive strength. The procedure integrates artificial neural network (ANN) model developed based on a comprehensive data base from literature, data clustering, and parameter optimization techniques to enhance accuracy and reliability. Experimental validation is undertaken to demonstrate the mix design determination procedure’s capability to accurately predict mix designs for geopolymer concrete based on the target compressive strength, validating its efficacy for mix proportion determination. The integration of chemical oxide content in fly ash, curing time, curing temperature, and activator properties results in a 15.9% improvement in prediction accuracy for the training dataset and a 68.3% enhancement for the testing dataset, compared to the base ANN model that includes only the weight of fly ash and activator properties. Employing data clustering techniques enables the identification of prior estimates for the mix design parameters related to specific fly ash types and target compressive strength, streamlining the mix design process by analyzing pertinent data subsets. Parameter optimization ensures refined mix proportions, achieving the desired target strength economically while minimizing material waste and cost. The development of a user interface facilitates easy manipulation of mix designs, catering to users of varying expertise levels. Additional options for deeper insights into geopolymer concrete characteristics can be integrated into the mix design determination procedure. To assess the mix design determination procedure's ability to generalize effectively, a variety of fly ash samples with distinct chemical compositions were utilized, differing from those already present in the database. This approach allows for a thorough evaluation of the mix design determination procedure's performance when presented with fly ash compositions it has not encountered before. By doing so, this provides insights into the adaptability of the mix design determination procedure beyond the limitations of the training and testing datasets.

EFEKTIVITAS PENAMBAHAN SERAT POLYPROPYLENE TERHADAP KUAT LENTUR BETON GEOPOLIMER dengan SUPERPLASTICIZER

Geopolymer concrete is concrete composed of a mixture of coarse and fine aggregate without Portland cement binder. Fly ash is used as a substitute, because it contains a lot of silica and alumina. Geopolymer concrete has good strength and durability, but concrete also has disadvantages, namely brittleness, workability and often cracking due to tensile loads. Superplasticizer (SP) is used at 2% of fly ash weight to improve workability. The SP used is Sika viscocrete 1003. The addition of polypropylene fiber is one way to overcome brittle properties and the appearance of fine cracks in concrete. In this paper using Sika fiberforce 48mm polypropylene fiber which has a tensile strength of 465N/mm2(MPa) at a dose of 0%, 0.5%, 1%, 1.5% and 2%. The alkaline activator used is a mixture of NaOH and Na2SiO3 solutions. The molarity of the NaOH solution used is 8M with a ratio of NaOH/Na2SiO3 2: 3. From the test results, it is known that the influence of polypropylene fiber is effective for increasing the bending strength of geopolymer concrete. The bending strength value of geopolymer concrete with the addition of polypropylene fibers increased by 60.19%. The polypropylene fiber content will be maximum at a percentage of 0.5% of the total volume of the mixture.Keywords: Fiber, Flexural Strength, Fly Ash Geopolymer Concrete, Polypropylene, Superplasticizer

Assessment of grain quality traits in mutant lines of winter barley

This study aimed to evaluate the variation among mutant lines derived from the winter barley variety Gigga, focusing on crucial feed quality traits such as protein, lysine, ash, fat, fiber content, and 1000-grain weight. Significant differences were identified in specific traits among the mutant lines, revealing promising candidates for further breeding programs. Mutant lines Gigga-25, Gigga-26, and Gigga-29 exhibited significantly higher protein content in their grains compared to the parent variety Gigga. In addition, Gigga-10 demonstrated elevated lysine content, while Gigga-4, Gigga-12, and Gigga-20 displayed increased 1000-grain weight. Of particular interest was Gigga-20, standing out for its exceptional combination of high protein content and favorable 1000-grain weight. This dual advantage positions Gigga-20 as a prime candidate for further investigation and potential integration into breeding programs, aiming to develop barley varieties with superior feed quality. Furthermore, a promising balance was observed in mutant lines Gigga-25 and Gigga-28, showcasing favorable proportions of protein and lysine content. These mutants present additional options for incorporation into breeding initiatives directed at enhancing the overall nutritional quality of feed grains.

The effect of lightweight geopolymer concrete containing air agent on building envelope performance and internal thermal comfort

Innovative building materials are the primary focus for researchers seeking to reduce energy consumption and advance global sustainability initiatives. This target could accomplished by addressing environmental issues and confronting the adverse impact of climate change. In this regard, this paper used a combined experimental and simulation approach to evaluate the effectiveness of lightweight geopolymer concrete (LWGC) incorporating industrial waste of aluminum powder (AP) and ferrosilicon waste powder (FWP) for reducing the energy consumption in the building envelop. Seven concrete mixes for LWGC were prepared using an air operator. Three mixtures contain 0.1 %, 0.2 %, and 0.3 % AP (FA), and three mixtures contain 0.1 %, 0.2 %, and 0.3 % FWP by weight of fly ash. The workability, unit volume weight, compressive strength, thermal conductivity, and microstructure analysis of the LWGC were examined. In addition, energy simulations were performed to evaluate their ability to reduce energy use in residential buildings. The inclusion of AP and FWP in LWGC resulted in a significant decrease in the density of the material, which amounts to 55 % for AP0.3 and 35 % for FWP0.3. The diameter of the LWGC pore structure increased as the Ca/Si ratio was reduced. The AP and FWP materials reduced energy consumption for building envelope elements such as external walls and roof insulation by 14.5 %, resulting in a 9.2 % overall reduction compared to the traditional concrete mix as a control sample.

High-strength steel fibre-reinforced geopolymer concrete utilising recycled granite waste and rice husk ash

A limited number of research studies have managed to attain high-strength geopolymer concrete, especially by utilising waste materials. In this study, granite waste (GW) from the mining industry, which contaminates the surrounding environment and causes air pollution, and rice husk ash (RHA) from agricultural waste were used to produce high-strength steel fibre-reinforced geopolymer concrete (HSFRGC). To reduce natural sand consumption, GW was utilised as a fine aggregate. RHA was added at 0%, 1%, 3%, and 5% by weight of coal fly ash to enhance the properties of HSFRGC. In addition, hooked-end steel fibres were employed as reinforcement at 0%, 0.5%, and 1% by volume of the concrete. The objective of this study is to investigate the effect of incorporating GW and RHA into HSFRGC, examining properties such as workability, strength, durability, and microstructure. Additionally, an assessment was conducted on the environmental impact and cost-effectiveness of the HSFRGC system with additives to determine its feasibility for practical scenarios. The results showed that the addition of 1% RHA as an additive in the geopolymer system, which led to microstructural homogeneity in the geopolymer matrix, could effectively enhance the mechanical properties and durability. Moreover, the addition of hooked-end steel fibres along with 1% RHA further improved the mechanical performance compared to samples without steel fibres, increasing both compressive strength by 25% and flexural response by 70%. Meanwhile, incorporating 1% RHA in mixtures could assist in mitigating chloride migration by approximately 10% due to the filler effect occurring in the geopolymer matrix. Regarding to environmental impact and cost-effectiveness, the incorporation of 1% RHA and 0.5% steel fibre demonstrated a suitable balance, exhibiting eco-strength efficiency with an increase of 15% compared to plain geopolymer concrete, making it a reasonable choice for both environmental concerns and cost efficiency.

A novel approach to heavy metal immobilization in municipal solid waste incineration fly ash: Investigating the use of chicken eggshell waste and CaO addition

Municipal solid waste incineration (MSWI) fly ash is a significant byproduct of waste-to-energy processes, and poses substantial environmental and health hazards if not treated properly. The process of Stabilization/Solidification (S/S) efficiently immobilizes hazardous elements present in the fly ash through a combination of physical and chemical mechanisms. The objective of this research is to thoroughly examine the feasibility of using chicken eggshell waste powder as a stabilizing agent for heavy metals, specifically targeting Pb, in MSWI fly ash. Experimental analysis involved varying eggshell waste to fly ash weight proportions, extending from 0.0 (control group), 7:3 up to 9.5:0.5 ratio, with designated curing durations of 2 and 72 hours, and maintaining a liquid-to-solid (L/S) ratio at 1.0 mL/g. In the two-hour curing period, the immobilization efficiency of Pb reached 100% with the addition of only 1.5% eggshell waste. Optimal ratios for Pb stabilization in MSWI fly ash were identified: maximum 2% eggshell waste for 2-hour curing and 1.5% for 72 hours due to elevated Pb and Cd levels of 7:3 ratio. Adding CaO mitigated these levels, enhancing stabilization and compliance with landfill criteria. The results demonstrated that the addition of CaO led to efficient immobilization of Pb, achieving approximately 99%. In addition, the observed enhancement in compressive strength resulting from the incorporation of CaO highlights its effectiveness in enhancing the stabilizing process of MSWI fly ash. By demonstrating the effectiveness of eggshell waste and CaO in stabilizing hazardous elements, this study advocates for scalable solutions and further research on long-term stability and environmental impacts.

Development of Fly Ash and Light Weight Mineral Granules Based Thermal Insulated Ceramic Container Bases for Keeping Hot Pot &amp; Hot Bowl

Thermal insulation based product development is the most important concern in the present age because we are using so many types of heating and cooking and baking devices and electronic equipment like microwave ovens, induction cook tops, Oven toaster Grillers, Electric tandoor, Roti makers, domestic furnace and electronic coffee &amp; tea makers. When we take out our bowls and pots from the microwave ovens and such type of other kitchen devices then heat present in the walls of the containers like utensil, bowl &amp; pot and heat present in the cooked food damages the top of the table and table covers. In order to prevent such things there is a burning need of development of thermal insulation bases and mats.

269 Influence of herbal active D on nursery pig growth performance, bone characteristics and blood measures

Abstract Pigs [n = 2.268; L337×1050 PIC; initial body weight (BW) = 5.5 ± 0.17 kg] were used in a 42-d growth study to evaluate the effects of herbal active D (a source of calcitriol) on growth performance, bone characteristics, and serum criteria of nursery pigs. Pigs were weaned at approximately 21-d of age and randomly allotted to 1 of 3 dietary treatments in a randomized complete block design. There were 27 pigs/pen and 28 replications/treatment across 2 rooms with pens blocked by BW and weaning date. Treatment diets were corn-soybean meal-based and consisted of a control diet (1,650 IU/kg of vitamin D3), or the control diet with 120 or 200 mg/kg of herbal active D (Phytobiotics, St. Louis, MO). Blood samples were collected from 25 gilts/treatment on d 21 and 42 to assess 25(OH)D3 and cytokine concentrations. At the end of the study, 10 pigs/treatment were euthanized and the right fibula, metacarpal, 2nd rib, and 10th rib were collected to determine bone density, bone breaking strength, and percentage bone ash by utilizing the de-fatted processing method. Overall (d 0 to 42), there was a marginally significant (P = 0.067) reduction in gain to feed ratio (G:F) as herbal active D increased, but no effect on final BW, average daily gain (ADG) and average daily feed intake (ADFI), or mortality (P &amp;gt; 0.10; Table). For bone breaking strength and bone ash, there was no evidence of a treatment × bone interaction (P &amp;gt; 0.10). Percentage of bone ash increased linearly across all bones as herbal active D increased (P = 0.026). Treatment did not affect bone ash weight and breaking strength (P &amp;gt; 0.10). However, metacarpals and 10th ribs had the greatest bone ash weight followed by the fibula with 2nd rib having the least (P &amp;lt; 0.05). Metacarpals had greater breaking strength compared with all other bones, followed by the fibula and 10th rib, with the 2nd rib having the least (P &amp;lt; 0.001). There was a bone × treatment interaction for bone density, where increasing herbal active D increased bone density for the 2nd rib (P = 0.012), but there were no treatment differences for other bones (P &amp;gt; 0.10). Additionally, there was no difference (P &amp;gt; 0.10) between treatments for porcine circovirus type 2 S/P ratio, porcine reproductive and respiratory syndrome, Mycoplasma hyopneumoniae, 25(OH)D3 status or circulating cytokine concentrations except for IL-8 concentrations which increased linearly (P = 0.027) as herbal active D increased. Antibodies and cytokine concentrations were greater on d 21 compared with d 42 (P &amp;lt; 0.001), except for IL-1ra and IL-8 having no significant (P &amp;gt; 0.10) day effect. In summary, herbal active D had minimal impact on growth or serum parameters; however, increasing herbal active D increased percentage bone ash.

70 Determination of phosphorus release of Microtech phytase in nursery pig diets

Abstract A total of 320 barrows (DNA 241 × 600; initially 10.7 ± 0.21 kg) were used in a 21-d growth trial to determine the available P (aP) release curve for Microtech phytase (Guangdong VTR Bio-Tech Co, Ltd. Zhuhai, Guangdong, China). At approximately 21 d of age, pigs were weaned and randomly allocated to pens and fed a common diet for 18 d and then fed a P depletion diet (0.11% aP) for 3 d. On d 21 post-weaning, considered d 0 of the study, pigs were blocked by average pen body weight (BW) and randomly allotted to 1 of 8 dietary treatments with 5 pigs per pen and 8 pens per treatment. Dietary treatments were derived from a single basal diet and ingredients including phytase, monocalcium P, limestone, and sand were added to create the treatment diets. Treatments include 3 diets with increasing aP (0.11, 0.19 and 0.27%) using monocalcium phosphate that were used to develop the standard curve and 5 diets with 0.11% aP and increasing phytase (250, 500, 1,000, 1,500 and 2,000 FTU/kg) that were used to develop the aP release curve. Pigs were weighed weekly and feed disappearance was recorded per pen for the calculation of average daily gain (ADG), average daily feed intake (ADFI) and gain to feed ratio (G:F). At the conclusion of the experiment, 1 pig, closest to the mean weight of each pen, was euthanized for bone analysis. The right fibula, rib, and metacarpal were collected for the determination of bone density, bone ash weight, and bone ash percentage. Pigs fed diets with increasing aP from inorganic P had improved (quadratic, P ≤ 0.05) final BW, ADG, and G:F (Table). Pigs fed diets with increasing phytase had linearly increased (P &amp;lt; 0.01) final BW and ADFI and quadratically increased (P ≤ 0.04) ADG and G:F. Bone density, bone ash weight, and bone ash percentage increased linearly (P &amp;lt; 0.01) with increasing aP from inorganic P and increasing phytase. Results were used to determine release values for Microtech phytase for each response criteria as shown in the table. The aP release quadratically increased (P ≤ 0.02) with increasing phytase when growth performance was used as response criteria and linearly increased (P &amp;lt; 0.01) when bone characteristics were used as response criteria. The available P release curve developed for Microtech phytase for percentage bone ash using combined data generated from all three bones is: aP = (0.868 × FTU/kg) ÷ (9,599.511 + FTU/kg).

PSI-16 Effect of HiPhorius phytase on finishing pig growth performance and bone mineralization with the use of two diet formulation strategies

Abstract Two experiments were conducted to determine the effect of HiPhorius (dsm-firmenich, Parsippany, NJ) phytase on finishing pig growth performance, serum chemistry, bone mineralization, and carcass characteristics. In Exp. 1, 1,161 pigs (PIC 337 × 1050, initially 36.7 ± 0.48 kg) were used in a 105-d trial. There were 27 pigs per pen and 10 or 11 replications per treatment. Treatments consisted of: 1) Control diet with no added phytase and formulated to NRC (2012) requirement estimates for standardized total tract digestible (STTD) P; 2) 600 FYT/kg added phytase formulated to the same STTD P as the control diet considering a release of 0.13% STTD P and 0.095% STTD Ca; 3) 1,000 FYT/kg added phytase formulated to the same STTD P as the control diet considering release of 0.16% STTD P and 0.107% STTD Ca, and 4) high STTD P (no phytase; approximately 22% above NRC requirement estimates). All diets were formulated to a 1.30:1 STTD Ca:STTD P ratio, but analysis suggested all phases were greater than the formulated target. Overall, pigs fed NRC (2012) or increased STTD P had increased average daily gain (ADG) and hot carcass weight (HCW; P &amp;lt; 0.05) compared with pigs fed the treatments with added phytase. Pigs fed NRC (2012) STTD P had greater (P &amp;lt; 0.05) gain to feed ratio (G:F) compared with those with 1,000 FYT/kg with other treatments intermediate. In Exp. 2, 1,160 pigs (PIC 337 × 1050, initially 75.9 ± 1.32 kg) were used in a 58-d trial. There were 27 pigs per pen and 11 replications per treatment. Treatments were the same as in Exp.1 except diets were formulated to the same total Ca:P ratio [1.15:1 from 75 to 100 kg and 1.12:1 from 100 to 136 kg body weight (BW)] without a STTD Ca release consideration from phytase; however, diets analyzed with a wider Ca:P compared with the expected value (Table 1). Overall, there were no differences in ADG, average daily feed intake (ADFI) or G:F among treatments (P &amp;gt; 0.10). For pigs fed NRC or increased STTD P, there was an increase (P &amp;lt; 0.05) in metacarpal bone density, and a tendency for increased (P &amp;lt; 0.10) bone ash weight and percentage bone ash compared with pigs fed treatments containing phytase. In conclusion, regardless of diet formulation strategy, pigs fed diets with phytase had decreased growth performance (Exp.1) and bone mineralization (Exp.2), which was unexpected and could be a result of the nutrient release values attributed to the phytase being overestimated, not enough phytate-bound P that is phytase-sensitive present in the diets, or too wide of a Ca:P ratio leading to reduced phytase activity in the analyzed diet samples compared with the intended ratio based on diet formulation.

Utjecaj letećeg pepela i vlakana banane na mehaničke karakteristike betonskih opločnika

The use of concrete reinforced with natural fibres is globally considered a sustainable approach to infrastructure development. This study investigated the salient features of concrete paver blocks fabricated using fly ash, coal ash, superplasticiser, and banana fibres as fibre reinforcements. Banana fibres (15 mm in length) were prepared, followed by the fabrication and characterisation of concrete paver blocks. In the current study, banana fibre (0.5 %, 1 %, 1.5 %, 2 %, and 2.5 %) and admixture (0.5 % constantly) were used in different mix proportions in the fabrication of paver block to analyse the physicomechanical properties. The results demonstrated that the paver blocks containing 2 % modified banana fiber (with respect to weight of fly ash) show higher compressive strength (7.45 % for I-dumble and 17.12 % for zigzag paver blocks), flexural strength (14.99 % for I-dumble and 8.67 % for zigzag) and split tensile strength (12.182 % for I-dumble and 9.971 % for zigzag paver blocks), compared to other mix proportions. The use of modified banana fibres as reinforcement was found to be very effective in improving the mechanical properties and life span of paver blocks, which may lead to minimizing the preparation.

Enhancing fire resistance in geopolymer concrete containing crumb rubber with graphene nanoplatelets

Geopolymer concrete (GPC) has a tendency to spall and lose its strength at high temperatures, rendering it brittle. This research incorporates graphene nanoplatelets (GNPs) to enhance the fire resistance of GPC. A comprehensive experimental framework, comprising 20 distinct mix designs, was developed. Crumb rubber (CR) was substituted for natural sand in the mixture proportions, ranging from 10% to 30% by volume, while GNPs were incorporated in quantities ranging from 0.1% to 0.4% by weight of fly ash. The objective of this study is to assess the mechanical properties of these mixtures after exposure to 30, 60, and 90 min of ISO 834 standard fire. The properties of GPC were analyzed both before and after exposure to fire. The attributes and properties investigated include spalling, mass loss, stress-strain behavior under compression, compressive strength, compressive toughness, modulus of elasticity, and porosity. Furthermore, the study examined the interfacial interaction between the aggregates and the geopolymer matrix, as well as microstructural and morphological changes due to fire exposure, utilizing field emission scanning electron microscopy (FESEM). The results indicated that GNPs significantly mitigated the loss of post-fire compressive strengths of GPC. The Response Surface Methodology (RSM) was employed to develop statistical models and optimize the mixture proportions of CR and GNPs, aiming to maximize the compressive strength and the modulus of elasticity while minimizing the mass loss of GPC after exposure to fire. The optimization outcomes suggested that a mixture containing 10% CR and 0.3% GNPs represented the optimal composition for enhancing the fire resistance of GNP modified rubberized GPC.

Compressive Strength of Palm Ash Geopolymer Mortar against High Temperatures

Palm ash geopolymer mortar is an alternative material that can be used in construction materials. This study to analyze the physical characteristics of palm ash geopolymer mortar against the effects of high temperatures. The mortar sample will be designed with a geopolymer mixture consisting of sand, palm ash, alkaline activator solution (NaOH and Na2SiO3) as an adhesive paste to replace cement. The geopolymer composition will take into account the effect of palm ash with an alkaline activator solution, so the optimal composition will be a sample size of 5x5x5 cm. Then the sample is put into the oven furnace to give high temperature effect on the mortar with temperature variations of 100°C, 200°C, and 300°C, and a mixture of 10%, 20%, 30% portland composite cement substitution by weight of palm ash. The results on the temperature variation of the maximum value of compressive strength occurred at 200°C with 20% cement variation and the minimum compressive strength results occurred at 100°C for 10% cement variation. The effect of high temperature will have an effect on visually different physical characteristics. These resulted in the composition of the palm ash geopolymer components which would affect high temperatures which resulted in changes in the physical characteristics of the palm ash geopolymer mortar.

Loss of maternal calcitriol reversibly alters early offspring growth and skeletal development in mice.

Ablation of Cyp27b1 eliminates calcitriol but does not disturb fetal mineral homeostasis or skeletal development. However, independent of fetal genotypes, maternal loss of Cyp27b1 altered fetal mineral and hormonal levels compared to offspring of WT dams. We hypothesized that these maternal influences would alter postnatal skeletal development. Cyp27b1 null and WT females were mated to bear only Cyp27b1+/- offspring. Forty-eight hours after birth, pups were cross-fostered to dams of the same or opposite genotype that bore them. Maternal and offspring samples were collected on days 21 (weaning) and 42. Offspring measurements included minerals and hormones, BMC by DXA, ash weight and mineral content, gene expression, 3-point bending tests, and microCT. Maternal lactational behavior was evaluated. Milk was analyzed for nutritional content. At day 21, offspring fostered by nulls, independent of birth dam, had ~20% lower weight, BMC, ash weight, and ash calcium than pups fostered by WT dams. Adjustment for body weight accounted for the lower BMC but not the lower ash weight and ash calcium. Hormones and serum/urine minerals did not differ across offspring groups. Offspring fostered by nulls had shorter femurs and lower cortical thickness, mean polar moment of inertia, cortical area, trabecular bone volume, and trabecular number. Dam lactational behaviors and milk nutritional content did not differ between groups. At day 42, body weight, ash weight, lengths, BMC, and tibial bone strength were no longer different between pups fostered by null vs WT dams. In summary, pups fostered by Cyp27b1 nulls, regardless of birth dam, have proportionately smaller skeletons at 21d, impaired microstructure, but normal mineral homeostasis. The skeletal effects are largely recovered by day 42 (3 wk after weaning). In conclusion, maternal loss of calcitriol impairs early postnatal cortical bone growth and trabecular bone mass, but affected offspring catch up after weaning.

Explosion mechanism and volume estimation of volcanic ash during the eruption of Sinabung Volcano on February 19, 2018: Insight from kinematic GPS and seismic data

On February 19, 2018, Sinabung Volcano erupted explosively, destroying the lava dome and producing a new crater. This eruption was the largest one since it became active again in 2010. We processed kinematic GPS observation combined with seismic data to analyze the explosion process and calculate the volume change during the deflation stage. The time series from the results of GPS data processing for 12 min shows a pattern of inflation that lasts for 180 s followed by deflation. Observation data is divided into 3 phases of activity. The first and second phases are inflation with pressure source during eruption at a depth of 2.3 km below the summit, followed by the third phase of deflation at a shallow depth. The hypocenter of explosion earthquake also in the depth of 2.3 km beneath the crater. This study provides information about the magma dynamics during and after the eruption of Sinabung Volcano. The volume change of inflation and deflation calculated approximately 3 × 106 m3 and − 2.2 × 106 m3, respectively. The ash weight produced by the February 19, 2018 eruption from seismic and ground deformation during the deflation period is approximately 5.6 million tons or 0.006 km3, equivalent with VEI 2 Sinabung eruption.

Microwave and ultrasound-assisted natural deep eutectic solvents-based extraction of pectin from onion peel wastes

ABSTRACT Pectin was extracted from onion peels using microwave and ultrasound-assisted natural deep eutectic solvents (NADES) and compared with conventional acid-extracted pectin. The primary screening trials indicated that the suitable combination of hydrogen bond acceptor and donor was choline chloride and tartaric acid, respectively. The Box-Behnken design was further used to optimize processing conditions like microwave intensity (26.903 W/g), microwave processing duration (2.99 min), and sonication duration (10.18 min) for maximizing the pectin yield from onion peel powder. The moisture, ash, and equivalent weight of extracted pectin were in the range specified by the International Pectin Producers Association. The higher anhydrouronic acid content of the NADES-extracted pectin indicated the higher purity of pectin compared to the conventional acid-extracted pectin. The NADES-based extraction was found to be a promising system for extracting pectin with a higher degree of esterification from onion peels.

Effects of phytase supplementation on broilers fed with calcium and phosphorus-reduced diets, challenged with Eimeria maxima and Eimeria acervulina: influence on growth performance, body composition, bone health, and intestinal integrity

An experiment was conducted to evaluate the effects of phytase in calcium (Ca) and available phosphorous (avP)-reduced diet on growth performance, body composition, bone health, and intestinal integrity of broilers challenged with Eimeria maxima and Eimeria acervulina. A total of 672 14-day-old male broilers were allocated to a 2 × 4 factorial arrangement with 6 replicates per treatment and 14 birds per replicate. Two factors were Eimeria challenge and 4 dietary treatments: 1) a positive control (PC; 0.84% Ca and 0.42% avP); 2) a negative control (NC; 0.74% Ca and 0.27% avP); 3) NC + 500 FTU/Kg of phytase (NC + 500PHY); and 4) NC + 1,500 FTU/Kg of phytase (NC + 1500PHY). On d 14, birds in the Eimeria-challenged groups received a solution containing 15,000 sporulated oocysts of E. maxima and 75,000 sporulated oocysts of E. acervulina via oral gavage. At 5 d postinoculation (DPI), the challenged birds showed a higher (P < 0.01) FITC-d level than the unchallenged birds. While the permeability of the NC group did not differ from the PC group, the phytase supplementation groups (NC + 500PHY and NC + 1500PHY) showed lower (P < 0.05) serum FITC-d levels compared to the NC group. Interaction effects (P < 0.05) of Eimeria challenge and dietary treatments on feed intake (FI), mucin-2 (MUC2) gene expression, bone ash concentration, and mineral apposition rate (MAR) were observed. On 0 to 6 and 0 to 9 DPI, Eimeria challenge decreased (P < 0.01) body weight (BW), body weight gain (BWG), FI, bone mineral density (BMD), bone mineral content (BMC), bone area, fat free bone weight (FFBW), bone ash weight, bone ash percentage and bone ash concentration; and it showed a higher FCR (P < 0.01) compared to the unchallenged group. The reduction Ca and avP in the diet (NC) did not exert adverse effects on all parameters in birds, and supplementing phytase at levels of 500 or 1,500 FTU/Kg improved body composition, bone mineralization, and intestinal permeability, with the higher dose of 1,500 FTU/Kg showing more pronounced enhancements. There was an observed increase in FI (P < 0.01) when phytase was supplemented at 1,500 FTU/Kg during 0 to 6 DPI. In conclusion, results from the current study suggest that dietary nutrients, such as Ca and avP, can be moderately reduced with the supplementation of phytase, particularly in birds infected with Eimeria spp., which has the potential to save feed cost without compromising growth performance, bone health, and intestinal integrity of broilers.

DURABILITY OF ASPHALT CONCRETE MIXTURE (AC-WC) USING CRUMB RUBBER WASTE AS AN ADDITIONAL MATERIAL AND PALM FRUIT ASH AS FILLER

The mixture of road pavement layers that is commonly used in Indonesia is a mixture of Asphalt Concrete (AC-WC). Addition of wastecrumb rubber (crumb rubber) in asphalt concrete mixture is a solution for highway planning and reducing waste pollutioncrumb rubber (crumb rubber) produced from a rubber tire factory. The purpose of this research is to determine the maximum percentage addition crumb rubber (crumb rubber) in the AC-WC mixture withfiller palm fruit bunch ash and to determine the residual strength index (RSI) value of the mixture which has a durability value that meets the requirements of General Specifications for Highways 2018 Revision 2. This study uses bitumen content with the percentage of mixed wastecrumb rubber used in the determination of OAC of 0%, 3%, and 6% by weight of asphalt andfiller palm fruit bunch ash 4% by weight of one AC-WC sample. The results of this study indicate testing marshall on variations crumb rubber 0% (without crumb rubber) as a normal mixture and 3% has met the specifications while the 6% variation does not. Maximum test results on the combination crumb rubber 3% resulted in the highest stability value of 1,028.85 kg at 0.5 hour immersion. The results of the durability test showed that the RSI value with a variation of 0% and 3% only fulfilled the 3 hour immersion time.

Loss of 24-hydroxylated catabolism increases calcitriol and fibroblast growth factor 23 and alters calcium and phosphate metabolism in fetal mice.

Calcitriol circulates at low levels in normal human and rodent fetuses, in part due to increased 24-hydroxylation of calcitriol and 25-hydroxyvitamin D by 24-hydroxylase (CYP24A1). Inactivating mutations of CYP24A1 cause high postnatal levels of calcitriol and the human condition of infantile hypercalcemia type 1, but whether the fetus is disturbed by the loss of CYP24A1 is unknown. We hypothesized that loss of Cyp24a1 in fetal mice will cause high calcitriol, hypercalcemia, and increased placental calcium transport. The Cyp24a1+/- mice were mated to create pregnancies with wildtype, Cyp24a1+/-, and Cyp24a1 null fetuses. The null fetuses were hypercalcemic, modestly hypophosphatemic (compared to Cyp24a1+/- fetuses only), with 3.5-fold increased calcitriol, 4-fold increased fibroblast growth factor 23 (FGF23), and unchanged parathyroid hormone. The quantitative RT-PCR confirmed the absence of Cyp24a1 and 2-fold increases in S100g, sodium-calcium exchanger type 1, and calcium-sensing receptor in null placentas but not in fetal kidneys; these changes predicted an increase in placental calcium transport. However, placental 45Ca and 32P transport were unchanged in null fetuses. Fetal ash weight and mineral content, placental weight, crown-rump length, and skeletal morphology did not differ among the genotypes. Serum procollagen 1 intact N-terminal propeptide and bone expression of sclerostin and Blgap were reduced while calcitonin receptor was increased in nulls. In conclusion, loss of Cyp24a1 in fetal mice causes hypercalcemia, modest hypophosphatemia, and increased FGF23, but no alteration in skeletal development. Reduced incorporation of calcium into bone may contribute to the hypercalcemia without causing a detectable decrease in the skeletal mineral content. The results predict that human fetuses bearing homozygous or compound heterozygous inactivating mutations of CYP24A1 will also be hypercalcemic in utero but with normal skeletal development.