Ascorbic Acid Levels Research Articles

Antioxidant Effect of a Plant-Derived Extracellular Vesicles’ Mix on Human Skin Fibroblasts: Induction of a Reparative Process

Plant-Derived Extracellular Vesicles extracellular vesicles (PDEVs) from organic agriculture (without the use of pesticides and microbicides) contain high levels of antioxidants. Organic PDEVs have shown an increased antioxidant power compared to PDEVs from single plants, suggesting a synergistic effect of the bioactives constitutively expressed in the PDEVs from single fruits. With this study, we wanted to investigate the beneficial effects of a mix of PDEVs on human skin cells. We found detectable levels of citric acid, ascorbic acid, glutathione, catalase, and SOD in a mix of PDEVs deriving from five different fruits (grape, red orange, papaya, pomegranate, and tangerine). We then treated H2O2-conditioned fibroblasts with the mix of PDEVs. The results showed that the PDEVs’ mixture reverted the H2O2-induced redox imbalance, restoring mitochondrial homeostasis, with a strong reduction of mitochondrial anion superoxide and an increase in sirtuin levels. The antioxidant action was consistent with wound repair on a lesion produced in a fibroblast’s monolayer. This result was consistent with an increased level of vimentin and matrix metalloproteinase-9, whose expression is directly related to the efficiency of the reparative processes. These data support a beneficial role of PDEVs in both preventing and treating skin injuries through their potent antioxidant and reparative activities.

Carvacrol maintains antioxidant capacity in goji fruit by increasing the content of bioactive compounds

Fresh goji berries are prone to decay and spoilage, resulting in economic losses. We investigated the effects of carvacrol (CVR) on the natural disease incidence and quality of goji berries by fumigating with varying CVR concentrations (0.06, 0.12, and 0.24 μL/mL). The results showed that CVR treatment inhibited the natural decay of goji berries by enhancing their quality, color, and total soluble solids/titratable acidity ratio. Moreover, 0.12 μL/mL CVR was determined to be the optimal concentration, as it maintained the sensory quality (except for aroma) and inhibited the disease incidence and preliminary disease expansion of goji fruit inoculated with Alternaria alternata. The treated berries exhibited elevated total phenolic content, total flavonoid content, ascorbic acid, glutathione, melatonin, total chlorophyll, and carotenoid levels. Higher hydrophilic and lipophilic total antioxidant activities and free radical scavenging capacities were also observed in the treated berries. Targeted metabolomics showed that treatment with 0.12 μL/mL CVR increased the levels of four phenolic acids (caffeic acid, chlorogenic acid, ferulic acid, and protocatechuic acid) and two flavonoids (naringenin and quercetin) in goji berries, thereby activating the gene expression of the phenylpropanoid metabolic pathway. In summary, CVR treatment delayed fruit senescence and the decline in fruit quality, promoted the accumulation of bioactive compounds, and improved antioxidant capacity and disease resistance by activating the phenylpropanoid metabolic pathway.

In Vivo Assessment of an Antioxidant Hydrogel Vitreous Substitute.

The vitreous humor undergoes liquefaction with age, resulting in complications that may require a vitrectomy, or surgical removal of the vitreous from the eye. Silicone oil, a common vitreous substitute, lacks properties similar to the natural vitreous. In particular, it lacks antioxidants that may be necessary to reduce oxidative stress in the eye. The purpose of this study was to evaluate antioxidant-loaded hydrogel vitreous substitutes in a pilot invivo study. Ascorbic acid and glutathione were loaded into synthesized PEGDA hydrogels. Following vitrectomy, experimental antioxidant hydrogels or silicone oil were injected into one eye of rabbits, while the other eye served as untreated or sham control. Ophthalmic assessments, including electroretinography, were performed. Levels of glutathione and ascorbic acid were higher in the eyes treated with the antioxidant-loaded hydrogel vitreous substitute, although this was not found to be significant after 28 days. There were no statistically significant differences between groups with respect to clinical examination, and ocular health scores, electroretinograms, and histology were normal. These results indicate minimal concerns for the hydrogel formulation or high levels of antioxidants. Future research will assess the capability of vitreous substitutes to prolong antioxidant release, with the goal of minimizing cataract after vitrectomy.

GhGME31D identified to regulate AsA activation in response to alkali stress from GME gene family implications in cotton

Vitamin C, also referred to as ascorbic acid (AsA), is recognized for its capacity to cure and avert scurvy, and it is crucial for regular human growth and development. In various crops, AsA participates in stress response mechanisms mediated by abscisic acid and has been discovered to have a crucial function in the morphogenesis, growth, development, and production of male gametes in plants. GDP-D-mannose 3′,5′-epimerase (GME) is essential in the synthesis of vitamin C. Our research identified 91, 83, 51, and 46 genes, respectively, found in G. barbadense (GbGMEs), G. hirsutum (GhGMEs), G. arboretum (GaGMEs), and G. raimondii (GrGMEs). Plants resulting from VIGS infection with GhGME31D clearly showed yellowing, water loss and wilting of leaves and black spots on stems. Measurement of MDA and AsA levels indicated that the plants were more damaged. This indicates that AsA has a substantial impact on plant growth and development.

Effect of pullulan and pullulan-chitosan composite coating on the antioxidant activity, texture, color and shelf-life of a local cultivar of sweet cherry

This study explored the effects of pullulan and pullulan-chitosan composite edible coatings on the post-harvest quality of sweet cherries over 20 days of storage. We evaluated weight loss, total soluble solids (TSS), decay percentage, color value, texture and biochemical content, including ascorbic acid, total phenolic content (TPC), flavonoids, anthocyanins, antioxidant activity and enzyme activity. The results revealed that cherries with 3 % pullulan coating presented the lowest weight loss (25 % vs 26.33 % in the control) and decay percentage (25 % vs 47 % in the control) and maintained superior texture and stability compared with those of the control and other coatings. TSS increased less in the coated cherries than in the control cherries, with 3 % pullulan-treated cherries showing the least increase, indicating slower ripening. Pullulan coatings effectively reduced color changes, with the 3 % coating delaying darkening in cherries more than the other treatments did. Additionally, the pullulan coating preserved higher levels of ascorbic acid, phenolic compounds (TPC) and flavonoids and delayed the decrease in antioxidant activity. Notably, the 3 % pullulan-treated cherries maintained the highest antioxidant activity (88.97 %) and had the greatest TPC (2.79 mg GAE/g FW) at the end of storage. The activity of enzymes, particularly peroxidase (POD), increased less in coated cherries, highlighting the role of coatings in delaying oxidative stress. Overall, the pullulan coatings, especially at a 3 % concentration, were the most effective at extending the shelf-life and maintaining the quality of the sweet cherries. These findings suggest that the use of a pullulan-based coating is a promising approach for preserving fruit quality during storage, thereby extending shelf-life. These coatings can contribute to a reduction in food waste, improved consumer acceptability and improved economic gains by reducing postharvest losses and maintaining quality. To optimize pullulan-based coatings for commercial application, future research can focus on optimizing the formulation, consumer acceptability and scalability. Moreover, field trials to assess the effectiveness of these coatings in preserving the quality of cherry can be performed. By addressing these areas, future research can contribute to the development of sustainable options for preserving the quality and extending the shelf life of sweet cherries and other fruits.

Identification of Key Enzymes and Genes Modulating L-Ascorbic Acid Metabolism During Fruit Development of Lycium chinense by Integrating Metabolome, Transcriptome, and Physiological Analysis.

Lycium chinense is acknowledged for its substantial nutritional benefits, particularly attributed to the high levels of ascorbic acid (AsA) found in its fruits. The "Mengqi No.1" variety of L. chinense, which is cultivated in Qinghai, is known for its high yield and exceptional quality. We utilized the "Mengqi No.1" variety as experimental materials and combined metabolomic, transcriptomic, and physiological analyses to investigate the metabolites, genes, and enzymes related to AsA metabolism in L. chinense fruits. The results revealed nine differential metabolites associated with AsA metabolism in L. chinense fruits across three stages, including 1D-Myo-Inositol-1,4-Bisphosphate, D-Fructose, L-(+)-Arabinose, I-Inositol, L-Arabinitol, D-Galactose-1-P, lactose, α-D-Glucose, and D-Glucose-6-P. Notably, the contents of D-Glucose-6-P, D-Galactose-1-P, and D-Fructose were increased as the fruit developed. Additionally, fresh weight, longitudinal length, and radial width were increased, while the contents of AsA and DHA were decreased. GalDH and DHAR are critical enzymes for the accumulation of AsA and DHA, exhibiting positive correlation coefficient. Furthermore, PMM1, PMM5, GME2, and GME3 were identified as key regulatory genes in the L-Galactose pathway of AsA synthesis, influencing D-Galactose-1-P, D-Glucose-6-P, α-D-Glucose, and D-Fructose. DHAR1 and DHAR2 are considered key positive regulator genes of AsA and DHA in the AsA-GSH cycle. However, the majority of genes (nine) act as negative regulators of AsA and DHA. These findings provide a foundation for the understanding of the regulatory mechanism of AsA metabolism in L. chinense fruits and offer insights into the utilization of AsA from L. chinense.

Impact of polyethylene microplastics exposure on kallikrein-3 levels, steroidal-thyroidal hormones, and antioxidant status in murine model: protective potentials of naringin

The widespread presence of microplastics in the environment has raised significant concerns regarding their potential impact on human and animal health. Among various microplastic types, polyethylene microplastics (PE-MPs) are particularly prevalent due to the extensive use in packaging and consumer products. Exploring the uncharted therapeutic potentials of naringin, this study delves into its mitigating effects on disruptions in kallikrein-3 levels, steroidal-thyroidal hormone balance, and antioxidant defense triggered by PE-MPs exposure, paving the way for novel interventions in environmental toxin-induced endocrine and oxidative stress disorders. Male Wistar rats (n = 24) were randomly grouped into four: Control, PE-MPs (1.5 mg/kg), PE-MPs + NAR (1.5 mg/kg PE-MPs + 100 mg/kg NAR), and NAR (100 mg/kg). Hormonal and antioxidant parameters were assessed after 28 days of exposure. PE-MPs exposure caused a significant increase(p < 0.005) in the level of kallikrein-3 (KLK-3) while it significantly reduces the levels of testosterone (TST), luteinizing hormone, thyroid stimulating hormone (TSH) and Free-triiodothyronine (fT3) and Total cholesterol (TChol) concentration. PE-MPs exposure also disrupted significantly (p < 0.005) antioxidant profile by down-regulating the activities of glutathione-S-transferase, catalase (CAT), superoxide dismutase (SOD) and reducing levels of glutathione (GSH) and ascorbic acid (AA) while concentration of malondialdehyde (MDA) levels were increased relative to control. However, the mitigating potentials of naringin on disruptions in hormonal and antioxidant profiles caused by PE-MPs exposure were demonstrated, as NAR normalized KLK-3, steroid, and thyroid hormone levels, cholesterol concentration, and enhanced antioxidant defense. This suggests that NAR is a promising protective agent against endocrine and oxidative damage induced by environmental contaminants such as microplastics.

Assessment of bioactive compounds, antioxidant properties and morphological parameters in selected microgreens cultivated in soilless media

The study investigated the effect of soilless media (burlap), on the morphological traits and antioxidant activities of microgreens from Brassicaceae, Amaranthaceae, and Linaceae families. The results revealed significant variations were observed in the selected morphological, biochemical composition, and antioxidant capacity of the microgreens. The radish sango and microgreens showed superior morphological characteristics compared to other microgreens. The elemental composition analysis revealed consistent moisture, ash, fat, fiber, and protein content across all families. The results revealed significant variations in the biochemical composition and antioxidant capacity of the microgreens, depending on the growing medium and between microgreens. Notably, microgreens differed in photosynthetic pigment profiles, with flaxseed and cabbage showing the highest chlorophyll content of 26.59 to 27.18 µg/g, FW and carotenoid content in a range of 3.74 to 6.39 µg/g, FW was observed in microgreens. The radish sango and beetroot microgreens exhibited elevated anthocyanin levels of 27.94–28.25 µmol/100 g, FW. Biochemical analysis indicated varying levels of ascorbic acid (177.58 to 256.46 mg/100 g, FW), total glucosinolate content (4.09 to 47.38 µmol/g, FW), phenolic content (131.44 to 298.56 mg GAE/100 g, FW), and flavonoid content (10.94 to 18.14 mg QUE/100 g, FW) were observed in selected microgreens families. Radish sango microgreens demonstrated the highest DPPH (76.82%, FW) and ABTS (88.49%, FW) radical scavenging activities, indicating superior antioxidant potential. The study showed that Brassicaceae microgreens are particularly rich in bioactive and antioxidant properties. Additionally, studies could assess the economic feasibility and scalability of soilless cultivation methods for microgreens to support their inclusion in sustainable agricultural practices and health-promoting diets.

Application of antioxidant peptide‐based coating on fresh‐cut apple

SummaryFresh‐cut apples are susceptible to enzymatic browning and spoilage. The objective of this study was to evaluate the effect of different coatings on fresh‐cut apples and develop a predictive model for their shelf life. The apples were treated with antioxidant peptide from Candida utilis (CUH), carboxymethyl chitosan (CMCS), and composite coatings, and their physicochemical properties were subsequently evaluated. Key factors identified through correlation analysis of shelf life were used as input parameters for a partial least squares regression (PLSR) model to predict the shelf life of fresh‐cut apples treated with different coatings. The results indicate that CUH, CMCS, and CUH‐CMCS coatings effectively delay the deterioration of fresh‐cut apples. Notably, the CUH‐CMCS composite coating demonstrated superior performance, showing only a slight 3.50% increase in the browning index (BI) during storage. Minimal changes were observed in weight loss and firmness, while overall total acidity (TA) and pH exhibited slight decreases. Moreover, the levels of ascorbic acid (Vc), polyphenol oxidase (PPO), and peroxidase (POD) were significantly lower compared to the control group, and the total bacterial count increased by no more than 0.7 log CFU g−1. The PLSR model accurately predicted the shelf life of fresh‐cut apples treated with different coatings, with and values both exceeding 0.90. The research results indicate that the coating and model developed in this study offer a novel approach for preserving and managing fresh‐cut apples.

Tert-Butylhydroquinone alleviates a postharvest pericarp browning of longan fruit by regulating antioxidant metabolism.

Harvested longan fruit is prone to pericarp browning, which restricts preservation quality and shortens fruit shelf life. The antioxidant system can defend against oxidative stress-mediated quality deterioration such as fruit browning. This study aimed to evaluate the effect of tert-butylhydroquinone (TBHQ) on anti-browning ability of longan fruit in association with redox metabolism. The results indicated that the application of 0.02% TBHQ significantly suppressed the progression of pericarp browning. In comparison with control, TBHQ treatment decreased the contents of hydrogen peroxide (H2O2), superoxide radical (O2 -⋅), and malondialdehyde, and retained high levels of ascorbic acid (AsA), glutathione (GSH), total phenolics as well as 1,1-diphenyl-2-picrylhydrazyl scavenging rate. Enhanced enzymatic activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), glutathione reductase (GR), monodehydroascorbate reductase, and dehydroascorbate reductase (DHAR), but decreased activities of polyphenol oxidase and peroxidase were also observed in TBHQ-treated fruit. Gene expression analysis indicated that redox metabolism-related genes, including DlSOD, DlCAT, DlGR, and DlAPX, were upregulated after TBHQ treatment. Correlation analysis suggested that antioxidants, including AsA, GSH, CAT, APX, SOD, and DHAR, were negatively correlated to reactive oxygen species production and percarp browning. These results suggest that TBHQ is effective in alleviating pericarp browning by increasing antioxidant capacity of longan fruit.

Yeast cell biocarrier for the encapsulation of ascorbic acid: effect of plasmolysis process, suspension media and ascorbic acid levels on the physicochemical, morphological and bioactive properties of microcapsules.

Ascorbic acid is a water-soluble vitamin and shows weak stability against external factors such as heat, oxygen, light etc. Due to its lower stability, encapsulation is an effective process for the preservation of its activity. Although there are a wide variety of encapsulation methods, the technique of encapsulation with yeast cells has been followed with increasing interest in recent years. In this study, encapsulation possibilities of ascorbic acid by yeast cells were investigated. In this context, Saccharomycess cerevisiae yeast cells in plasmolyzed and non-plasmolyzed forms were used in two different suspension media (water and ethanol) and effect of ascorbic acid concentrations (10, 20 and 50 g per 10 g yeast) were studied. A total of 12 different yeast microcapsule samples were produced and some physicochemical, bioactive and structural characterizations were performed. The ascorbic acid level of yeast microcapsule samples was determined as 206.4-713.9 and 202.8-726.1 mg g-1 for plasmolyzed and non-plasmolyzed yeast cell types, respectively. ABTS radical scavenging activity increased from 27.23 to 233.04 μg TE g-1 by increased ascorbic acid levels. Ascorbic acid capsules were used in soft candy processing against free ascorbic acid and it was found that 47.9% ascorbic acid loss was detected for control sample at the 24-day storage while the ascorbic acid loss was approximately 25% for yeast microcapsules. It was concluded that yeast cells are capable of preserving ascorbic acid stability during storage and yeast cells can be used effectively and safely for the manufacturing of the ascorbic acid microcapsules. © 2024 Society of Chemical Industry.

Evaluation of SO2, glutathione, and glutathione-rich inactive dry yeast as antioxidants on the fermentation properties of Roxburgh rose wine

Cili (Roxburgh rose) wine, which is rich in ascorbic acid, flavones, and polyphenols, presents unique challenges in wine production, including oxidation, aroma loss, and SO2 residues. This study investigated the effects of three antioxidants (SO2, glutathione (GSH), and GSH-rich inactive dry yeast (g-IDY)) on the fermentation properties of cili wine and evaluated the potential of g-IDY for addressing the aforementioned issues. g-IDY significantly enhanced the fermentation rate, as evidenced by increased CO2 production within 48 h, and maintained high levels of GSH, total phenolics, total flavones, and ascorbic acid, contributing to the antioxidant capacity and overall stability of the wine. g-IDY also improved the retention and concentration of key aromatic compounds, including higher alcohols, esters, and terpenes, thereby enhancing the sensory profile of the wine. Principal component analysis confirmed that different antioxidants significantly altered the volatile profile, with g-IDY demonstrating distinct improvements. The optimal concentration was 400 mg/L g-IDY. Compared with traditional antioxidants like SO2 and pure GSH, g-IDY offers a safer and more effective solution for preserving the quality of cili wine. This study provides experimental evidence for refining the fermentation process and promotes the use of g-IDY for enhancing the antioxidant capacity and sensory attributes of cili wine.

Green fabrication of biomass-derived carbon dots and bio-based coatings: Potential of enhancing postharvest quality on Chinese flowering cabbage

The objective of this study was to develop a bio-nanocomposite coating (CQSC) by combining chitosan quaternary ammonium salt (CQAS) and sericin (SC) with biomass-derived carbon dots (CDs) to extend the shelf life of Chinese flowering cabbage (CFC). The effects of different concentrations of CDs (0.2, 0.4, 0.6, 0.8, and 1.0 mg/mL) on the physicochemical, structural, and functional activity of nanocomposite particles were evaluated. CQAS exhibited strong inhibitory effects against Escherichia coli and Bacillus subtilis. Moreover, the application of CQSC on CFC significantly reduced mass losses, slowed the increase in lignin content, maintained ascorbic acid and chlorophyll levels, inhibited the growth of microorganisms, and preserved the unique texture and aroma of CFC during storage at 10 °C compared with uncoated CFC. The results will contribute to the further development of CDs coatings to improve the postharvest preservation effect of fruits and vegetables.

Effect of chlorine dioxide (ClO2) combined with different oxygen permeability package treatments on postharvest antioxidant activity and storage life of ‘Liaofeng’ grapes

To enhance the storage quality of ‘Liaofeng’ grapes, this research employed chlorine dioxide in collaboration with different package of preservative bags to explore its preservative effect on ‘Liaofeng’ grapes. After fumigating the grapes with 300 mg L−1 ClO2 solution, the control group was packaged in unpacked and PE bags, while the treatment group was packaged in three kinds of bags with different oxygen permeability, and stored in 4 °C and relative humidity controlled at 90%–95% for 90 days. The results showed that ClO2-GB package treatment significantly delayed the decline of grape hardness (P < 0.05), and decreased the weight loss, threshing, decay and peduncle browning index after storage for 90 days. ClO2-GB package treatment not only diminishes the levels of soluble solids content, titrable acidity, ascorbic acid and glutathione but also effectively suppresses the respiration rate in fruits. The contents of total phenols, flavonoids, anthocyanins and reduced glutathione after ClO2-GB treatment were significantly higher than those in unpackaged group (P < 0.05), and curbed the increase in relative electrical conductivity and malondialdehyde concentration. In comparison to ClO2-YTB and ClO2-YB treatments, ClO2-GB treatment demonstrated superior efficacy in modulating the antioxidant metabolism of grapes. It notably elevated the enzymatic activities of catalase, superoxide dismutase, ascorbate peroxidase, and glutathione reductase, while inhibiting the activities of peroxidase and polyphenol oxidase. The quality assessment of ‘Liaofeng’ grapes after 90 d storage period, conducted through principal component analysis (PCA), yielded the following ranking: ClO2-GB > ClO2-YB > ClO2-YTB > ClO2-PE > ClO2. This suggests that 300 mg L−1 ClO2 fumigation combined with different package treatments was more beneficial to the storage of ‘Liaofeng’ grape. ClO2-GB package treatment emerged as the most advantageous.

Machine learning and multiple linear regression models can predict ascorbic acid and polyphenol contents, and antioxidant activity in strawberries.

Strawberry is a rich source of antioxidants, including ascorbic acid (ASA) and polyphenols, which have numerous health benefits. Antioxidant content and activity are often determined manually using laboratory equipment, which is destructive and time-consuming. This study constructs a prediction model for antioxidant compounds utilizing machine learning (ML) and multiple linear regression based on environmental, plant growth and agronomic fruit quality-related parameters as well as antioxidant levels. These were studied in three farms at two-week intervals during two years of cultivation. During the ML model screening, artificial neural network (ANN)-boosted models displayed a moderate coefficient of determination (R2) at 0.68-0.78 and relative root mean square error (RRMSE) at 3.8-4.8% in polyphenols and total ASA levels, as well as a high R2 of 0.96 and low RRMSE at <3.0% in antioxidant activity. Additionally, we developed variable selection models regarding the antioxidant activity, and variables two and five (environmental parameters and leaf length, respectively) with high accuracy were selected. The linear regression analysis between the actual and predicted data of antioxidants in the ANN-boosted models revealed high fitness with all parameters in almost all training, validation and test sets. Furthermore, environmental parameters are essential in developing such reliable models. We conclude that ANN-boosted, stepwise and double-Lasso regression models can predict antioxidant compounds with enhanced accuracy, and the relevant parameters can be easily acquired on-site without the need for any specific equipment. © 2024 Society of Chemical Industry.

Exogenous nitric oxide treatment delays the senescence of postharvest mung bean sprouts by regulating ascorbic acid metabolism.

This study evaluated the effects of nitric oxide (NO) treatment on ascorbic acid (AsA) metabolism and mung bean sprout quality. It examined changes in the AsA content, enzyme activity associated with AsA metabolism, antioxidant capacity, cell membrane composition, and cellular structure to clarify the effects of NO on mung bean sprouts. Nitric oxide treatment preserved mung bean sprout quality by enhancing significantly the activity of enzymes involved in the l-galactose pathway (including guanosine diphosphate (GDP)glutathione (-d-mannose pyrophosphorylase, GDP-mannose-3',5'-epimerase, GDP-l-galactose phosphorylase, l-galactose-1-phosphate phosphatase, l-galactose dehydrogenase, and l-galactose-1,4-lactone dehydrogenase) and the AsA-glutathione (GSH)(Beijing Solarbio Science and Technology Co.,Ltd., Beijing, China) cycle (including ascorbate peroxidase, ascorbic acid oxidase, glutathione reductase, dehydroascorbate reductase, and monodehydroascorbate reductase) during the germination and storage stage. Increased enzyme activity led to an increase in AsA content and enhanced antioxidant capacity, and reduced the membrane lipid damage in mung bean sprouts. This was demonstrated by higher levels of DPPH radical scavenging capacity, unsaturated fatty acids and phospholipids, along with lower levels of hydrogen peroxide, superoxide anions, and malondiadehyde, in NO-treated mung bean sprouts. Scanning electron microscopy also revealed that NO treatment maintained the integrity of the cellular structure of the mung bean sprouts. Nitric oxide accelerates AsA metabolism effectively by regulating the biosynthesis and regeneration of AsA in mung bean sprouts. These changes increased AsA levels, alleviated membrane lipid damage, delayed senescence, and maintained the quality of mung bean sprouts during storage. © 2024 Society of Chemical Industry.

Synergistic effects of dietary selenium nanoparticles and vitamin C improve growth performance, immune response, and antioxidant status of juvenile common carp (Cyprinus carpio)

IntroductionThis study aimed to investigate the synergistic effects of dietary selenium nanoparticles (Se-NPs) and vitamin C (VC) on growth, body composition, antioxidant defense, immunity, and serum biochemical indexes of common carp (Cyprinus carp) juveniles. MethodologyThe test diets were supplemented with three levels of Se-NPs (0, 0.5, and 1 mg/Kg) and three levels of VC (0, 500, and 1000 mg/Kg): the basal diet without supplemental Se-NPs and VC (VC0SeNPs0; control), 0.5 mg Se-NPs /Kg (VC0SeNPs0.5), 1 mg Se-NPs /Kg (VC0SeNPs1), 500 mg VC/Kg (VC500SeNPs0), 1000 mg VC/Kg (VC1000SeNPs0), 500 mg VC/Kg and 0.5 mg Se-NPs (VC500SeNPs0.5), 1000 mg VC/Kg and 0.5 mg Se-NPs (VC1000SeNPs0.5), 500 mg VC/Kg and 1 mg Se-NPs (VC500SeNPs1), 1000 mg VC/Kg and 1 mg Se-NPs (VC1000SeNPs1). The fish were randomly divided into nine experimental groups in triplicate tanks per treatment and fed on their respective diets for 60 days. ResultsThe findings displayed that fish fed with VC500SeNPs1 and VC500SeNPs0.5 diets had significantly (P < 0.05) higher specific growth rates when compared to other groups. The lowest feed conversion ratio was detected in the VC1000SeNPs1 group and the highest in the control group (P < 0.05). VC, Se-NPs, and their interaction had no significant effect on serum malondialdehyde, ACH50, and IgM (P > 0.05). However, the best parameters associated with antioxidant capacity (higher serum levels of superoxide dismutase and total reduced glutathione) and physiological status (higher concentration of serum globulin and lower amounts of aspartate aminotransferase and lactate dehydrogenase) belonged to the VC1000SeNPs1 and VC500SeNPs1 groups. The results suggest that the Se-NPs and VC combination more efficiently influence the common carp’s growth performance, antioxidant status, immunity, and physiological parameters. ConclusionOverall, the diet enriched with 500 mg VC and 1 mg Se-NPs /Kg (VC500SeNPs1) is suitable for boosting the growth and immunity of common carp.

A high-performance wearable microneedle sensor based on a carboxylated carbon nanotube-carbon nanotube composite electrode for the simultaneous detection of uric acid and dopamine

Uric acid (UA) and dopamine (DA) are crucial biomarkers in the human body. However, there is limited research on detecting UA or DA in subcutaneous interstitial fluid (SISF), and no studies on their simultaneous detection in SISF. Here, we developed an electrochemical microneedle sensor based on the carboxylated carbon nanotubes/multiwalled carbon nanotubes (CCNT/CNT) composite electrode for the simultaneous detection of UA and DA. Highly carboxylated CCNT was prepared and mixed with CNT and organosilicon-modified acrylic resin to form the CCNT/CNT composite. The carboxyl functional groups of CCNT enhance the electrode’s adsorption of UA and DA, while CNT improves electron conduction. The sensor achieves a wide linear detection range for UA (5–600 μM) with high sensitivity (7.13 μA μM−1 cm−2) and linearity (R2 = 0.994), as well as a wide linear range for DA (2–200 μM) with high sensitivity (13.31 μA μM−1 cm−2) and linearity (R2 = 0.994). The sensor achieved simultaneous detection of DA and UA in artificial interstitial fluid (ISF), with sensitivity and linearity remaining nearly unchanged compared to that in PBS, and is not affected by high levels of ascorbic acid (AA). Finally, the sensor successfully detected changes in UA levels in ISF of subjects before and after alcohol consumption in vivo, demonstrating its practical application capabilities. This study presents a practical strategy for detecting human biomarkers characterized by low cost, easy fabrication and excellent performance of electrode materials, and a well-designed microneedle sensor preparation scheme, making it highly promising for further research extension.

Field-grown lettuce production optimized through precision irrigation water management using soil moisture-based capacitance sensors and biodegradable soil mulching

AbstractScientists, environmentalists, and farmers are currently in pursuit of sustainable agricultural practices that can effectively ensure global food security while simultaneously mitigating environmental degradation. A field experiment was conducted to elucidate the impact of low-cost capacitance soil moisture-based sensors on lettuce (Lactuca sativa L.) irrigation water conservation, agro-physiological aspects, and nutritional characteristics. The experiment also involved the use of five different types of soil mulching films: white geotextile (WGup), green geotextile (GGup), black plastic (BPup), white geotextile for both above and below ground (WGup-down), green geotextile for both above and below ground (GGup-down), in addition to un-mulched soil (control). The findings demonstrated that the application of WGup, BPup, WGup&amp;down, and GGup&amp;down resulted in a significant improvement in irrigation water conservation, with WGup exhibiting the highest savings at 41.86%, while the control group exhibited the least amount of water savings at 19.87%. Moreover, the highest productivity levels were observed in plants mulched with GGup&amp;down, reaching 47,944.68 kg ha−1, whereas the lowest productivity was recorded in plants mulched with green geotextile GGup at 22,377.89 kg ha−1. In terms of irrigation water productivity (IWP), the order of effectiveness was BPup &gt; GGup-down &gt; WGup &gt; WGup-down &gt; GGup &gt; Control, with BPup achieving the highest IWP at 60.19 kg m−3 and the control treatment reporting the lowest at 27.80 kg m−3. The percentage of the irrigation water applied for crop evapotranspiration (Irc) showed that the control treatment saved the least amount of irrigation water, saving only 19.87%, while the best treatment was WGup, achieved the highest percentage of irrigation water savings at 41.86%. Additionally, mulched plants exhibited higher levels of nutrients (N, P, Ca, Mg, Fe, Mn, and Zn), ascorbic acid (AsA), and total phenol content (TPC), while showing lower nitrate content in the leaves compared to non-mulched plants. Overall, the utilization of soil moisture-based capacitance sensors and biodegradable mulching films has proven to be highly effective and low cost by 16.633 US$ year−1 to enhance irrigation water productivity, growth performance, nutritional quality, and overall productivity of lettuce crops, thereby contributing to the sustainability of lettuce production in arid regions.

Preharvest methyl jasmonate application delays cell wall degradation and upregulates phenolic metabolism and antioxidant activities in cold stored raspberries

The effects of preharvest methyl jasmonate (MeJA) spray application on the physicochemical quality, metabolism of phenolics, and cell wall components in raspberries were investigated during a 10-day cold storage period. MeJA spray reduced firmness loss, decay incidence, and weight loss, while maintained higher levels of soluble solids content, ascorbic acid, anthocyanins and flavonoids in raspberries. Furthermore, MeJA application resulted in increased total pectin and protopectin levels, as well as lowered water-soluble pectin, and activities of pectin methyl esterase, polygalacturonase and cellulase enzymes. Additionally, MeJA treatment upregulated the phenylpropanoid pathway, leading to higher endogenous phenolics and activities of phenylalanine-ammonia lyase and shikimate dehydrogenase. In conclusion, preharvest MeJA spray application could be adopted to enhance the storage potential of cold-stored raspberries for 10 days by maintaining higher firmness, assuring better physicochemical quality, and increasing phenolic metabolism, while reducing cell wall hydrolysis.