Ascardia Galli Research Articles

Anthelmintic Effects of Betel Nut and Tamarind Seeds to Ascaradia galli Worms

The main focused of the study is to evaluate the Anthelmintic efficacy of the Betel nut and Tamarind seeds to Ascaradia galli worms in vitro in comparison to commercial anthelmintic. The study was distributed to 8 treatments, which consisted of 5, 10 and 15 grams of betel nut and tamarind seeds, commercial anthelminintic control and negative control treatment. The motility time, changes in sizes and microscopic changes at 24,48 and 72 hours was observed. Results revealed that Betel nut at 15 grams were the most effective as the Ascardia galli worms were dead at 48 hours of observation compared to other treatments. Therefore, using Betel nut at 15 grams was comparable to commercial anthelmintic treatment and it is recommended to utilize to eliminate and as worm killer to Ascaradia galli.

Prevalence of parasites in selected captive bird species.

Blood and fecal samples of chukar partridge (Alectoris chukar), albino pheasant (Phasianus colchicus), silver pheasant (Lophura nycthemera), rose-ringed parakeet (Psittacula krameri) and turkeys (Meleagris gallopavo) were analyzed to check parasitic prevalence. To record parasites these five avian species were placed kept in separate cages at Avian Conservation and Research Center, Department of Wildlife an Ecology, University of Veterinary and Animal Sciences, Lahore, Pakistan. 100 fecal and 100 blood samples for each bird species were inspected to analyze internal parasites. During present study, 17 species of endoparasites 14 from fecal samples and three from blood were examined. Two species of ectoparasites i.e. mite Dermanyssus gallinae 42% and fowl ticks Args persicus 41%were studied. Blood parasites included Plasmodium juxtanucleare 50%, Leucoctoyzoon simond having parasitic prevalence 40%, and Aegyptinella pullorum having parasitic prevalence of 40%. Parasitic species recorded from fecal samples included 6 species of nematodes viz. Allodpa suctoria 2%. Syngamus trachea with parasitic prevalence of 60%, Capillaria annulata 37.5%, Ascardia galli 24%, Capillaria anatis 40% and Heterakis gallinarum 28.3%. Similarly, two species of trematodes viz. Prosthogonimus ovatus having parasitic prevalence of 50% and Prosthogonimus macrorchis 21% were also documented from fecal avian samples . Single cestode species Raillietina echinobothrida having parasitic prevalence of 72% and 3 protozoan species i.e. Eimeria maxima having parasitic prevalence of 21%, Giardia lamblia 41% and Histomonas meleagridis 18% were documented during corpological analysis. In our recommendation, proper sanitation, medication and vaccination of bird's enclousres are suggested to avoid parasites.

Studies on parasitic prevalence in pet birds from Punjab, Pakistan.

During this one year study, blood and fecal samples of doves (Zenaida asiatica), ducks (Anas platyrhynchos), pigeons (Columba livia), partridges (Alectoris chukar), turkeys (Meleagris gallopavo) and goose (Chen caerulescens) were collected to assess the parasitic prevalence in these birds. The birds were kept at Avian Conservation and Research Center, Department of Wildlife and Ecology, University of Veterinary and Animal Sciences, Lahore. All these avian species were kept in separate cages and their entire body was inspected on regularly basis to record external parasites. For internal parasites, 100 blood and 100 fecal samples for each species were analyzed. During present study, two species of ectoparasites i.e. fowl ticks (Args persicus) and mite (Dermanyssus gallinae) while 17 species of endoparasites; three from blood and 14 from fecal samples were identified. Prevalence of blood parasites was Plasmodium juxtanucleare 29.3%, Aegyptinella pullorum 15% and Leucoctoyzoon simond 13%. Parasitic species recorded from fecal samples included 6 species of nematodes viz. Syngamus trachea with parasitic prevalence of 50%, Capillaria anatis 40%, Capillaria annulata 37.5%, Heterakis gallinarum 28.3%, Ascardia galli 24% and Allodpa suctoria 2%. Similarly, two species of trematodes viz. Prosthogonimus ovatus having parasitic prevalence of 12.1% and Prosthogonimus macrorchis 9.1% were also recorded from fecal samples of the birds. Single cestode species Raillietina echinobothrida having parasitic prevalence of 27% and 3 protozoan species i.e. Eimeria maxima having prevalence 20.1%, Histomonas meleagridis 8% and Giardia lamblia 5.3% were recorded. In our recommendation, proper medication and sanitation of the bird's houses and cages is recommended to avoid parasites.

The effect of isolated quercetin of the leaf extract of schleichera oleosa (lour) oken on ascardia galli

The effect of isolated quercetin of the leaf extract of schleichera oleosa (lour) oken on ascardia galli

Quantitative estimation of phytoconstituents and in vitro anthelmintic assessment of leaf extracts of tagetes erecta Linn.

Tagetes erecta Linn. (Asteraceae) is an ornamental plant and has versatile medicinal importance. The present study has been designed to assess in-vitro anthelmintic activity of different leaf extracts along with qualitative and quantitative estimation of phytoconstituents of Tagetes erecta Linn. By following the standard methods, different physicochemical parameters, UV fluorescence study and preliminary phytochemical screening have been examined and estimation of total tannins, flavonoids and phenolic compounds have been percformed. Different concentration (25, 50, 100 mg/mL) of ethanolic and aqueous extracts of the leaf have been used for the activity against all groups of earthworms (Pheretima posthuma, Eisenia fetida, Perionyx excavates) and nematode (Ascardia galli), collected from the freshly slaughtered Gallus gallus, while Albendazole and 3% Tween 80 in normal saline have been taken as standard and control respectively. Significant amount of tannins (4.05±2.15 mg TAEg), flavonoids (21.80±2.01 mg QEg) and phenolics (40.19±1.67 mg GAEg) were found in ethanolic extract. All the extracts exhibited potent anthelmintic activity in dose dependent manner; the ethanolic extract at 100 mg/mL concentration showed more significant activity against all earthworms and nematode comparing against the aqueous extract.

Comparative incidence of Helminth parasites in domestic fowl, white leg horne, layer and cock

Investigation on gastrointestinal helminths was conducted on 20 domestic fowl, 20 white leghorn, 20 layer and 20 cock of Dhaka city. Six species of helminth belonging to trematode, cestode and nematode were found to infect the different parts of alimentary canal of domestic fowl. The prevalence of helminths in domestic fowl were as follows: Catatropis verrucosa (25%), Amoebotaenia sphenoides (10%), Hymenolepis cantaniana (35%), Rallietina echinobothrida (55%), Ascardia galli (70%) and Heterakis gallinarum (30%) whereas, in white leghorn the prevalence of Rallietina echinobothrida (20%) and Ascardia galli (35%) were recorded. In layer, higher prevalence of Rallietina echinobothrida (55%) and Ascardia galli (60%) were recorded; in cock the prevalence of specific helminthes were as follows: Hymenolepis cantaniana (10%), Rallietina echinobothrida (40%), Cotugnia digonopora (20%), Ascardia galli (60%) and Heterakis gallinarum (25%). Along with the prevalence and intensity of infestation of different species of parasites, intensity were also recorded. Highest number of parasites were collected from intestine.Bangladesh J. Zool. 44(2): 245-254, 2016

Phytochemical and Anthelmintic Investigation of Leaves of Glochidion ellipticum Linn

Worm infections remain a major constraint to livestock productivity across all agro-ecological Zones. Now, the main mode for control of gastrointestinal parasites is based on the synthetic anthelmintic. However, wide spread increase of anthelmintic resistance, scarcity and high cost especially to farmers of low income in developing countries led to the need of other alternative helminth control methods. Among other alternative methods, there is considerable and expanding interest in traditional herbal de wormers. In present communication methanolic and aqueous extracts of leaves of Glochidion ellipticum Linn. Flem were investigated for their anthelmintic activity against Pheretima posthuma and Ascardia galli. Different concentrations were used in the assay, which involved paralysis and death time of the worms. Both the extracts showed significant anthelmintic activity.

Comparative Anthelmintic Efficacy of Caesalpinia Crista, Nigella Sativa and Oxfendazole in Broilers with Experimentally Induced Ascaridia Galli Infection

The study was conducted to explore the effect of two herbal drugs, Caesalpinia crista and Nigella sativa in comparison with Oxfendazole in broilers experimentally induced with Ascaridia galli infection .A total of 130 day old broiler chicks were divided into five groups i.e. A, B, C, D and E each group having 26 birds. Group E was kept as negative control, while all other groups were induced with experimental Ascardia galli infection on 15 th day. Group A was kept as positive control and groups B and C were treated with Caesalpinia crista and Nigella sativa as methanolic extract orally once @50mg/kg body weight and group D was medicated with Oxfendazole @10mg/kg body weight orally once on 25 th day post infection. On 25th day post infection before treatment 4 birds from each group were slaughtered, and worm count was done. The efficacy was confirmed by counting the worm, slaughtering all the bird on 30 th day post infection. The mean worm count was significantly different (p<0.05) between the treated and non treated groups. The efficacy of the plant extracts were less as compare to Oxfendazole. The efficacy of Caesalpinia crista and Nigella sativa was 70.83% and 57.5% respectively while the efficacy of Oxfendazole was 90.83%.In vitro treatment at 37±1°C revealed that a serial dose of the plant extract caused dose-dependent vermicidal effect on the worm (significant efficacy at P<0.05), except at the lowest dose (i.e. 5 mg/mL). The efficacy of drugs was calculated on the basis of reduction in total worm count after treatment.

Cytotoxic and anthelmintic potential of crude saponins isolated from Achillea Wilhelmsii C. Koch and Teucrium Stocksianum boiss.

BackgroundSaponins isolated from plant sources have a number of traditional and industrial applications. Saponins have pharmacological effects like anti-inflammatory, molluscicidal, antimicrobial, antispasmodic, antidiabetic, anticancer, anticonvulsant, anthelmintic, antitussive and cytotoxic activities. The current work describes the anthelmintic and cytotoxic activities of crude saponins of Achillea Wilhelmsii and Teucrium Stocksianum as these plants are rich with saponins.MethodsBrine shrimp cytotoxic activity of crude saponins was determined by Meyer et al. (1982) at test concentrations of 1000 μg/ml, 100 μg/ml, 10 μg/ml, 7.5 μg/ml, 5.0 μg/ml, 2.5 μg/ml and 1.25 μg/ml. Percentage mortality of test concentrations was determined. Similarly, in vitro anthelmintic activity was determined against roundworms, tapeworms and earthworms. Albendazole and piperazine citrate at concentration 10 mg/ml were used as standard anthelmintic drugs.ResultsCrude saponins of Achillea wilhelmsii (CSA) and Teucrium stocksianum (CST) had, respectively, cytotoxic activity with LC50 values 2.3 ± 0.16 and 5.23 ± 0. 34 μg/ml. For in vitro anthelmintic activity, time for paralysis and death of parasites (parasiticidal activity) was noted. At concentration 40 mg/ml, crude saponins of Achillea wilhelmsii are 1.96 and 2.12 times more potent than albendazole against Pheretima posthuma and Raillietina spiralis, respectively. Similarly, at concentration 40 mg/ml, crude saponins of Teucrium stocksianum (CST) has 1.89, 1.96 and 1.37 times more parasiticidal activity than albendazole against Pheretima posthuma, Raillietina spiralis and Ascardia galli, respectively.ConclusionCrude saponins of Achillea wilhelmsii and Teucrium stocksianum have cytotoxic and anthelmintic activity. The crude saponins may be excellent sources of cytotoxic and anthelmintic constituents that warrant its isolation and purification for new drug development.

Anthelmintic activity of Clerodendrum viscosum

The juice of the fresh leaves of Clerodendrum viscosum is widely used in the treatment of helminthiasis in folklore medicine. In this present study we have investigated the folklore claim methodically and scientifically. The ethanolic and aqueous extracts of leaves and roots of the plant are tested against Pheretima posthuma & Ascardia galli to ascertain their anthelmintic potential. All the extracts showed significant anthelmintic activity in dose dependent manner; the ethanolic extract of root at a dose of 200 mg/ml exhibited better anthelmintic activity than the reference drug, Piperazine citrate (10 mg/ml) against both type of worms.

&lt;i&gt;In vitro &lt;/i&gt; anthelmintic activity of &lt;i&gt; Baliospermum montanum &lt;/i&gt; muell. arg roots

Alcohol and aqueous extracts from the roots of Baliospermum montanum Muell. Arg were investigated for their anthelmintic activity against Pheretima posthuma and Ascardia galli. Various concentrations (10-100 mg/ml) of each extract were tested in the bioassay, which involved determination of time of paralysis and time of death of the worms. Both the extracts exhibited significant anthelmintic activity at highest concentration of 100 mg/ml. Piperazine citrate (10 mg/ml) was included as standard reference and distilled water as control.

Evaluation of Bauhinia variegata Linn Stem Bark for Anthelmintic and Antimicrobial Properties

This research work was taken up to investigate the anthelmintic and antimicrobial potential of the stem bark of Bauhinia variegata Linn. The crude ethanolic extract of the plant was evaluated for anthelmintic activity using Indian earthworm Pheretima posthuma and Ascardia galli as test worms. Various concentrations (10-100 mg/ml) of ethanolic extract were tested in the bioassay, which involved determination of time of paralysis (P) and time of death (D) of the worms. Piperazine citrate (10mg/ml) included as reference standard. Antimicrobial activity of the crude ethanolic extract was evaluated by agar disc diffusion method and Minimum Inhibitory Concentration (MIC) was determined by broth dilution method by using some bacterial and fungal strains of microorganism. Ciprofloxacin (5μg/ml) and Cotrimazole (25μg/ml) were used as reference standard for the antibacterial and antifungal studies respectively. The ethanolic extract showed promising anthelmintic and antimicrobial activities and results were comparable with the standards included in the study.

In Vitro. Screening of Cleome Viscosa. Extract for Anthelmintic Activity

The crude alcohol and aqueous extracts of the seeds of Cleome viscosa. Linn. (Capparidaceae) were investigated for their anthelmintic activity against Pheretima posthuma. and Ascardia galli.. Various concentrations (10–100 mg/mL) of each extract were tested in the bioassay, which involved determination of time of paralysis and time of death of the worms. Both extracts exhibited considerable anthelmintic activity in a dose-dependent manner. The most significant activity was observed at the highest concentration of 100 mg/mL against both types of worms. Piperazine citrate (10 mg/mL) was included as standard reference and distilled water as control.

Prevalence of gastro-intestinal parasites in captive birds of Gujarat zoos

Received on 16 August 1999 Accepted on 3 March 2000 Abstract A total of 106 group faecal samples of different wild birds from Kamal Nehru Zoo, Ahmedabad and Sayyajibaug Zoo, Vadodara, were examined. Out of this 51 (48.11%) were found positive for parasitic infection. Eggs of Ascaris and Capillaria species were observed in 22 (20.75%) and 14 (13.2%) group faecal samples respectively, while the oocyst of Coccidia (Eimeria species) were observed in 19 (17.92%) group faecal samples. Ascardia galli and Cotugnia digonopora were recovered during postmortem of three hariyal pigeon, while only Ascaridia galli was observed in a postmortem of parrot, peacock and cockatiel.