Mercury is still used in the amalgamation process in small-scale gold processing. This study aims to evaluate the mercury contamination of various environmental media in the artisanal small-scale gold mining (ASGM) area in the Simpenan District, Sukabumi Regency. The ecological media examined in this study included groundwater, river water, soil, sediment, biota, and plants. The threshold number was determined using APHA 3125 and Indonesian National Standard (SNI) 7387:2009. Meanwhile, the mercury concentration in the ambient air was measured directly using a mercury analyser. Mercury concentrations in the water, soil, sediment, biota, and plant matrices were calculated using a calibration curve. The study found that the levels of mercury in groundwater, river water, and sediment were all below the applicable quality standards of <0.0001, <0.0001 and 0.001 mg/L, respectively. However, the levels of mercury in soil and plants were 27.28 and 0.52 mg/kg, respectively. Additionally, the levels of mercury in four types of fish found in a river adjacent to the ASGM location, namely beunteur (0.33 mg/kg), snakehead (0.05 mg/kg), catfish (0.26 mg/kg) and eel (0.20 mg/kg), exceeded the specified quality standards. In conclusion, mercury contamination is present in the soil, plants, and aquatic biota (fish) in the ASGM area in the Simpenan District, Sukabumi Regency.