Artificial Supplementation Research Articles

Iron biofortification in cereal crops: Recent progress and prospects

AbstractMicronutrient malnutrition is one of the major causes of human disorders in the developing world. Iron (Fe) is an important micronutrient due to its use in human metabolism such as immune system and energy production. Estimates indicate that above 30% of the global population is at risk of Fe deficiency, posing a particular threat to infants and pregnant women. Plants have adapted various strategies for uptake, transport, accumulation, and storage of Fe in tissues and organs which later can be consumed by humans. Biofortification refers to increase in micronutrient concentration in edible parts of plants and understanding the pathways for Fe accumulation in plants. Conventional plant breeding, transgenics, agronomic interventions, and microbe‐mediated biofortification are all potential methods to address Fe deficiency. This review article critically evaluates key aspects pertaining to Fe biofortification in cereal crops. It encompasses an in‐depth analysis of the holistic presence of Fe, its significance in both human and plant contexts, and the diverse strategies employed in Fe uptake, transport, accumulation, and storage in plant parts destined for human consumption. Additionally, the article explores the bioavailability of Fe and investigates strategies for biofortification, with a specific emphasis on both traditional methods and recent breakthroughs aimed at enhancing the Fe content in food crops. Keeping in view the significance of Fe for human life, appropriate biofortification strategies may serve better to eliminate hidden hunger rather than its artificial supplementation.

Comparative Study of High-Resolution LysB29(Nε-myristoyl) des(B30) Insulin Structures Display Novel Dynamic Causal Interrelations in Monomeric-Dimeric Motions

The treatment of insulin-dependent diabetes mellitus is characterized by artificial supplementation of pancreatic β-cell ability to regulate sugar levels in the blood. Even though various insulin analogs are crucial for reasonable glycemic control, understanding the dynamic mechanism of the insulin analogs may help to improve the best-protracted insulin analog to assist people with type 1 diabetes (T1D) to live comfortably while maintaining tight glycemic control. Here, we present the high-resolution crystal structure of NN304, known as insulin detemir, to 1.7 Å resolution at cryogenic temperature. We computationally further investigated our crystal structure’s monomeric-dimeric conformation and dynamic profile by comparing it with a previously available detemir structure (PDB ID: 1XDA). Our structure (PDB ID: 8HGZ), obtained at elevated pH, provides electrostatically triggered minor movements in the equilibrium between alternate conformational substates compared to the previous structure, suggesting it might induce an intermediate state in the dissociation pathway of the insulin detemir’s hexamer:dihexamer equilibrium. Supplemented with orientational cross-correlation analysis by a Gaussian network model (GNM), this alternate oligomeric conformation offers the distinct cooperative motions originated by loose coupling of distant conformational substates of a protracted insulin analog that has not been previously observed.

Involvement of hypoxia-inducible factor activity in inevitable air-exposure treatment upon differentiation in a three-dimensional keratinocyte culture.

Formation of the human skin epidermis can be reproduced by a three-dimensional (3D) keratinocyte culture system, in which air-exposure is inevitable upon initiation of differentiation. In the continuous submerged culture without air-exposure, even with a differentiation-compatible medium, several keratinocyte-specific proteins were not induced resulting in the formation of aberrant epidermal layers. To clarify the mechanism by whichair-exposure promotes keratinocyte differentiation, we performed a comparative analysis on biological properties between submerged and air-liquid interphase culture systems. By transcriptomic analysis, hypoxia-inducible factor (HIF)-related genes appeared to significantly change in these cultured cells. In submerged culture, the transcriptional activity of HIF on its canonical response element was enhanced, while air-exposure treatment drastically reduced the transcriptional activity despite the high HIF protein level. Regulating HIF activity through reagents and genetic manipulation revealed that the reduced but retained HIF-transcriptional activity was essentially involved in differentiation. Furthermore, we showed, for the first time, that artificial supplementation of oxygen in the submerged culture system could restore keratinocyte differentiation as observed in the air-exposed culture. Thus, we mechanistically evaluated how HIF regulates the air-exposure-dependent differentiation of keratinocytes in a 3D culture system.

The Academy of Breastfeeding Medicine27th Annual International MeetingBaltimore, MarylandSeptember 15–18, 2022

The Academy of Breastfeeding Medicine27^th Annual International MeetingBaltimore, MarylandSeptember 15–18, 2022

Abstract P1015: Construction Of A Pro-cardiogenic Extracellular Matrix: How Stem-cell Derived Epicardium Enhances Cardiac Regeneration Through Fibronectin

Background: Human embryonic stem cell-derived epicardium (hESC-Epis) has been shown to improve the function of hESC-derived cardiomyocytes (hESC-CM) in vitro and post-transplantation into animal myocardial infarction models. However, the underlying mechanisms mediating these effects remain poorly characterised. Bulk RNA sequencing of hESC-Epis suggests a role for genes related to extracellular matrix remodeling in mediating these effects, with fibronectin (FN1) being highly implicated. Study Objective: To examine the role of FN1 in hESC-CM/Epi crosstalk through gain and loss of function studies. Methods: A tetracycline inducible FN1 knockdown system was used on hESC-CMs and Epis within the setting of 3D-engineered heart tissue. Changes in contractility and calcium handling were measured. Recombinant human plasma FN1 (rhFN) was then used to determine the effect of FN1 supplementation on hESC-CM function. Results: FN1 knockdown in hESC-Epis resulted in significant abrogation of the beneficial effects on hESC-CM force generation and calcium handling. Knockdown of FN1 in hESC-CMs however significantly attenuated contractility of the hESC-CMs but had no effects on calcium handling. When rhFN was added to hESC-CMs in increasing doses, hESC-CM contractility was improved in a dose dependent fashion to the same extent as seen with hESC-Epis co-cultures. However, rhFN supplementation did not result in significant improvements in calcium handling. Conclusions: hESC-CM and Epi crosstalk improving hESC-CM function may rely heavily on the presence of FN1. FN1 secreted by both cell types are shown to be of importance in this crosstalk with artificial supplementation of rhFN being able to recapitulate some beneficial effects of hESC-Epis on hESC-CMs. Further studies are required to determine the role of specific isoforms of secreted FN1 on hESC-CM maturity and what mechanism underlies these effects. This opens up the possibility of improving cell-based therapy for heart failure post-myocardial infarction using recombinant FN1 supplementation.

Zinc supplementation modifies brain tissue transcriptome of Apis mellifera honeybees

BackgroundBees are the most important group of pollinators worldwide and their populations are declining. In natural conditions, Apis mellifera depends exclusively on food from the field to meet its physiological demands. In the period of scarcity, available resources are insufficient and artificial supplementation becomes essential for maintaining the levels of vitamins, proteins, carbohydrates, and minerals of colonies. Among these minerals, zinc is essential in all living systems, particularly for the regulation of cell division and protein synthesis, and is a component of more than 200 metalloenzymes.ResultsThe total RNA extracted from the brain tissue of nurse bees exposed to different sources and concentrations of zinc was sequenced. A total of 1,172 genes in the treatment that received an inorganic source of zinc and 502 genes that received an organic source of zinc were found to be differentially expressed among the control group. Gene ontology enrichment showed that zinc can modulate important biological processes such as nutrient metabolism and the molting process.ConclusionsOur results indicate that zinc supplementation modulates the expression of many differentially expressed genes and plays an important role in the development of Apis mellifera bees. All the information obtained in this study can contribute to future research in the field of bee nutrigenomics.

Improving Soybean Production Using Light Supplementation at Field-Scale: A Case Study

In modern agriculture, there is a growing need for increasing crop efficiency while minimizing environmental impacts. The use of high-efficiency light supplementation to enhance plant development is limited for high-productive crops at field conditions (outdoor). This study evaluated the soybean plant’s yield responses in an open commercial area (field scale) cultivated under conditions of artificial light supplementation. A commercial irrigated (pivot) area received an illumination system for light supplementation (LS) in its inner pivot spans. About 40 hours of LS were applied to the plants during the soybean crop cycle. The area’s outer pivot spans did not receive light supplementation (nLS). The internode number, the plant height, the pods per plant were evaluated weekly to compute the area under the progress curve (AUPC). The grain yield at harvest was also assessed. The AUPC of the internode number, plant height and pods per plant were positively affected by the LS treatment. The regular soybean cycle (nLS) is about 17 weeks; however, the LS harvest occurred three weeks later. Light supplementation increased soybean grain yield by 57.3% and profitability by 180% when compared to nLS. Although light supplementation at field scale poses a challenge, it is now affordable since sustainable field resistant technologies are now available. The present study is the first known report of light supplementation used to improve soybean crop production at field scale.

Improving Soybean Production Using Light Supplementation at Field-Scale: A Case Study

In modern agriculture, there is a growing need for increasing crop efficiency while minimizing environmental impacts. The use of high-efficiency light supplementation to enhance plant development is limited for high-productive crops at field conditions (outdoor). This study evaluated the soybean plant’s yield responses in an open commercial area (field scale) cultivated under conditions of artificial light supplementation. A commercial irrigated (pivot) area received an illumination system for light supplementation (LS) in its inner pivot spans. About 40 hours of LS were applied to the plants during the soybean crop cycle. The area’s outer pivot spans did not receive light supplementation (nLS). The internode number, the plant height, the pods per plant were evaluated weekly to compute the area under the progress curve (AUPC). The grain yield at harvest was also assessed. The AUPC of the internode number, plant height and pods per plant were positively affected by the LS treatment. The regular soybean cycle (nLS) is about 17 weeks; however, the LS harvest occurred three weeks later. Light supplementation increased soybean grain yield by 57.3% and profitability by 180% when compared to nLS. Although light supplementation at field scale poses a challenge, it is now affordable since sustainable field resistant technologies are now available. The present study is the first known report of light supplementation used to improve soybean crop production at field scale.

Explainable Automated Essay Scoring: Deep Learning Really Has Pedagogical Value

Automated essay scoring (AES) is a compelling topic in Learning Analytics for the primary reason that recent advances in AI find it as a good testbed to explore artificial supplementation of human creativity. However, a vast swath of research tackles AES only holistically; few have even developed AES models at the rubric level, the very first layer of explanation underlying the prediction of holistic scores. Consequently, the AES black box has remained impenetrable. Although several algorithms from Explainable Artificial Intelligence have recently been published, no research has yet investigated the role that these explanation models can play in a) discovering the decision-making process that drives AES, b) fine-tuning predictive models to improve generalizability and interpretability, and c) providing personalized, formative, and fine-grained feedback to students during the writing process. Building on previous studies where models were trained to predict both the holistic and rubric scores of essays, using the Automated Student Assessment Prize's essay datasets, this study focuses on predicting the quality of the writing style of Grade-7 essays and exposes the decision processes that lead to these predictions. In doing so, it evaluates the impact of deep learning (multi-layer perceptron neural networks) on the performance of AES. It has been found that the effect of deep learning can be best viewed when assessing the trustworthiness of explanation models. As more hidden layers were added to the neural network, the descriptive accuracy increased by about 10%. This study shows that faster (up to three orders of magnitude) SHAP implementations are as accurate as the slower model-agnostic one. It leverages the state-of-the-art in natural language processing, applying feature selection on a pool of 1592 linguistic indices that measure aspects of text cohesion, lexical diversity, lexical sophistication, and syntactic sophistication and complexity. In addition to the list of most globally important features, this study reports a) a list of features that are important for a specific essay (locally), b) a range of values for each feature that contribute to higher or lower rubric scores, and c) a model that allows to quantify the impact of the implementation of formative feedback.

Enrichment of Different Plant Seeds with Zinc and Assessment of Health Risk of Zn-Fortified Sprouts Consumption

Zinc (Zn) is a nutrient that regulates many vital functions of the human body, hence the demand for Zn in the balanced daily nutrition has to be covered. Priming of seeds with Zn for Zn-enriched sprout production can be considered a good alternative to artificial supplementation in a modern diet. Hence, the aim of our study was to determine the exposure level of Zn bringing neither risk for humans nor growth inhibition of enriched broccoli, pea and sunflower sprouts. Seeds treated with 0, 10, 20 and 30 μg mL−1 ZnSO4 responded in a differentiated way to Zn. Pea seed germination and sprout growth was diminished by 30 μg mL−1 ZnSO4, but for sunflower sprouts this Zn level resulted in the highest fresh mass and largest hypocotyls. Zn content in sprouts greatly increased in a dose-dependent manner, mostly in broccoli (up to 25 times) and peas (up to 4 times), and to a lesser extent (up to 120%) for sunflowers. Free radical scavenging activity was usually decreased. Considering the potential non-carcinogenic risk of sprouts consumption estimated by the hazard analysis, the safest exposure level for seed priming will be 10 μg mL−1 ZnSO4. It was confirmed by random amplification of polymorphic DNA analyses indicating no DNA variations in sprouts treated with 10 μg mL−1 ZnSO4 compared to the control.

PIH58 THE EFFECT OF LONG-LASTING INTAKE OF MG AND B12ON THE MOTHER AND THE INTRA-UTERINE DEVELOPMENT OF THE FOETUS AS WELL AS THE OUTCOME OF DELIVERY

This current scrutiny aims at examining the frequency of both natural and artificial supplementation of magnesium and vitamin B12besides illuminating their effect on the health condition of pregnant mothers, the date of birth and corporal development of newborn infants. The quantitative, cross-sectional study was carried out in 2018 through an expedient, professional sample-taking. Those mothers formed the basis of the research who were raising children aged between 0 and 6 months (n=94). The individually-edited questionnaire was analyzed by Microsoft Excel beyond employing descriptive statistics along with c2 and Anova test (p<0.05). Responding mothers 87.23% of participating individuals had regularly taken foetus protection vitamins prior to pregnancy on a daily basis and ¾ of them were familiar with their content. Half the sample took vitamin B12 while ¾ of partakers used Mg daily throughout the three trimester. Food containing B12was represented in 2/5 of mothers’ everyday nutrition whereas proportionately 1/3 was encountered regarding Mg. In case of 1/5 of gravid individuals the appearance of the symptoms of preeclampsia was experienced during pregnancy in addition depression was perceived concerning 1/5 of participants following delivery, yet neither of these formerly viewed factors indicated connection with the frequency of intake of either the natural or artificial Mg (c2 test p>0.05). The frequency concerning the supplementation of natural or artificial Mg and vitamin B12 produced their effect neither on the means of delivery nor the date of birth and corporal parameters of the newborn infant (Anova test p>0.05). Gravid women considered the supplementation of vitamins and minerals significant prior to, during and following pregnancy yet there is a demand on the increase of the informative tasks of the members of the prenatal team regarding both artificial and natural intake focusing on the latter area.

Wintering home range and habitat use of the whooper swans (Cygnus cygnus) in Sanmenxia Wetland, China

AbstractsHome range and habitat use are the central components of avian ecology, and studies on these aspects will be helpful for the conservation and management of avian populations. Sixty‐seven swans were global positioning system tagged at Sanmenxia Wetland of Henan Province to obtain detailed location data in winter from 2015 to 2016. The home range size of swans was the largest in the middle wintering period and followed by the early period and late period, and sizes were significantly different among the three wintering periods. There were significant differences in habitat use among the different wintering periods. In the early period, the swans mainly used the aquatic grasses and emergent plants zones, and they mainly relied on artificial supplementation because of a lack of natural feeding habitats in the middle period. In the late period, the swans mainly used the newly emerged terrestrial grasses zone. Except for the deep water, the use of other water levels was significantly different among the different wintering periods. In the early wintering period, the swans tended to prefer the low and high water level areas; in the middle period, they were mainly in the intermediate and high water level areas and they used all the water level areas except the deep water level in the late wintering period. It was concluded that some plants are preferred by the swans, such as reeds, cattails and barnyard grass, and that water depth should be suitable for the swans, with the water levels varying over a gradient.

Effects of urea foliar application on growth and yield of green pepper

Vegetable crops require nutrients in various quantities for growth, development, and reproduction. Plants nutrients might be naturally available from soil or/ and atmosphere. However, modern cultivation needs artificial supplementation of nutrients. Green pepper is an important vegetable crop in several countries including Afghanistan. Irregular rates and methods of fertilizers application in green pepper production caused its yield to decrease. A study was conducted at the experimental farm of agriculture faculty; Nangarhar University in 2017 with traditional method (control), 1%, 2% and 3% of urea foliar application treatments under the randomized complete block design, this study aimed to investigate and point out the best fertilizer application rate and method for green pepper production in Afghanistan, especially Nangarhar province. The control treatment increased plant height, fruit length, fruit weight, fruit yield and consequently net income compared to other treatments. However, 3% treatment had negligible differences with control; although 1% and 2% treatments got third and fourth positions, respectively. In 3% treatment, the urea was efficiently utilized with lower urea losses compared to the control treatment. Further researches should undertake to find out the proper application rates and methods for other chemical fertilizers.

Genetic decline, restoration and rescue of an isolated ungulate population.

Isolation of small populations is expected to reduce fitness through inbreeding and loss of genetic variation, impeding population growth and compromising population persistence. Species with long generation time are the least likely to be rescued by evolution alone. Management interventions that maintain or restore genetic variation to assure population viability are consequently of significant importance. We investigated, over 27 years, the genetic and demographic consequences of a demographic bottleneck followed by artificial supplementation in an isolated population of bighorn sheep (Ovis canadensis). Based on a long‐term pedigree and individual monitoring, we documented the genetic decline, restoration and rescue of the population. Microsatellite analyses revealed that the demographic bottleneck reduced expected heterozygosity and allelic diversity by 6.2% and 11.3%, respectively, over two generations. Following supplementation, first‐generation admixed lambs were 6.4% heavier at weaning and had 28.3% higher survival to 1 year compared to lambs of endemic ancestry. Expected heterozygosity and allelic diversity increased by 4.6% and 14.3% after two generations through new alleles contributed by translocated individuals. We found no evidence for outbreeding depression and did not see immediate evidence of swamping of local genes. Rapid intervention following the demographic bottleneck allowed the genetic restoration and rescue of this bighorn sheep population, likely preventing further losses at both the genetic and demographic levels. Our results provide further empirical evidence that translocation can be used to reduce inbreeding depression in nature and has the potential to mitigate the effect of human‐driven environmental changes on wild populations.

The role of probiotics in women with recurrent urinary tract infections.

Urinary tract infections (UTIs) still represent a significant bother for women and result in high costs to the health system. Increasing antimicrobial resistance has stimulated interest in non-antibiotic prophylaxis of recurrent UTIs. Evidence shows that the microorganisms inhabit many sites of the body, including the urinary tract which has long been assumed to be sterile in healthy individuals, might have a role in maintaining urinary health. Studies of the urinary microbiota (UM) have identified remarkable differences between healthy populations and those with urologic diseases. The depletion of these organisms in women susceptible to UTIs raised the question of whether artificial supplementation of these microorganisms as probiotics could lower infection rates. In the literature, probiotic interventions were shown to have some efficacy in the treatment and prevention of urogenital infections. Despite previous controversy regarding the use of probiotics, as treatment for UTIs, there are increasing signs that it may be possible to use them as a first step in regulating the UM so as to reduce the risk of or as a treatment for certain urinary diseases. However, further future clinical trials, involving large numbers of patients, will be mandatory to achieve definite evidence of the preventive and curative role of probiotics in UTIs. Details about correct formulations in terms of amount of bacteria, viability and associated growth factors, will be required in order to standardize the administration schedule and achieve homogeneous and comparable results on selected patients.

Transdermal Delivery of Living and Biofunctional Probiotics through Dissolvable Microneedle Patches.

Bioactive functional probiotics play an important role in many health applications such as maintaining skin health and the immunity of the human host. Artificial supplementation of probiotics would enhance immune functions as well as regulate skin health. However, simple and effective methods to deliver probiotics into the dermis to regulate local dermal tissue are still lacking. Furthermore, microneedles have been used for transdermal drug delivery in a pain-free manner, yet there were few reported methods to deliver living microbes via microneedles. In this work, we developed a technique to deliver bioactive functional probiotics, using lactobacillus as the model probiotic, into local dermis by dissolvable microneedles. The transdermal delivery of probiotics might enhance local skin regulation and immunity, and dissolvable microneedles served as a safe and pain-free tool for dermal microbial delivery. Lactobacillus was encapsulated in dissolvable microneedles with high viability by a centrifugation casting method. The microneedles rapidly dissolved after skin penetration, releasing the lactobacillus into the subcutaneous space, without causing local tissue irritation. The lactobacillus was functionally bioactive following transdermal delivery, actively synthesizing lactic acid both ex vivo and in vivo. Our technique provided a safe, effective, and convenient approach for the transdermal delivery of probiotics into local skin, with the potential to improve skin health and immunity.

Conservation of Primula farinosa in Poland with respect to the genetic structure of populations

The bird’s-eye primrose (&lt;em&gt;Primula farinosa&lt;/em&gt; L.) is an endangered species in Poland. The sole remaining, and critically endangered, population of approximately 300 flowering plants is located in the Beskid Sądecki Mountains (Jaworki, Poland). The genetic investigation was performed using highly variable microsatellite markers on a total of four populations: the Polish population and its three nearest known Slovak counterparts. We hypothesize that the Polish population is a relic of the previously much wider Central European mountain/lowland range and is thus genetically distinct from the rest of the Slovak stock. Clarification of this issue is needed before active protection interventions such as artificial supplementation can be carried out. Our results, particularly those from STRUCTURE Bayesian clustering, showed clear population structure within the dataset: all three Slovak populations were dominated by one genetic group, while the Polish population comprised mostly individuals dominated by the second genetic group. Only limited gene flow was observed between the Polish and Slovak populations. This demonstrates the distinct genetic makeup of the Jaworki population, which is probably the result of prolonged isolation from the rest of the &lt;em&gt;P. farinosa&lt;/em&gt; range and admixture of genes from various lineages. Their origin could be determined by including plant material from the rest of the &lt;em&gt;P. farinosa&lt;/em&gt; range, i.e., Scandinavian, Baltic, and Alpine/Carpathian locations, in a future study. The immediate practical application of our results is the recommendation that all supplementation interventions to the Jaworki population must be limited to plants produced from locally collected seeds.

EFEITOS DO EXERCÍCIO FÍSICO SOBRE DIABETES MELLITUS TIPO 1: UMA REVISÃO SISTEMÁTICA DE ENSAIOS CLÍNICOS E RANDOMIZADOS

Type 1 Diabetes m ellitus (DM1) is characterized as chronic autoimmune pathology. Physical exercise (PE) is considered a positive strategy in DM1 therapy. The aim of the study was to systematize scientific evidence linking EF interventions in individuals with DM1. This is a systematic review of the literature, from clinical and randomized studies. A survey was carried out in indexed journals in the PUBMED, SCOPUS, COCHRANE, LILACS and SCIELO databases. The Descritors Health Sciences used were: diabetes mellitus , type 1 AND exercise and their correspondents in portuguese and spanish. We included 28 articles published between the years 2012 and 2016. All studies addressed the relationship between pathology management and different types, frequency, volume, duration and intensity of exercises. Different subjects were approached, such as: artificial pancreas, diet, supplementation, insulin and hypoglycemia. One of the great challenges to ensure good management of DM1 with EF practice is related to glycemic control and hypoglycemia during, shortly after or after intervention. The aerobic, resisted, pilates and intermittent PE, along with adequate diet, meal times and supplementation were effective in managing the various metabolic and clinical variables of patients with DM1.

A Qualitative Study of Social, Cultural, and Historical Influences on African American Women's Infant-Feeding Practices.

The purpose of this study was to describe cultural factors influencing African American mothers' perceptions about infant feeding. Analysis of six focus group discussions of diverse African American mothers yielded sociohistorical factors that are rarely explored in the breastfeeding literature. These factors are events, experiences, and other phenomena that have been culturally, socially, and generationally passed down and integrated into families, potentially influencing breastfeeding beliefs and behaviors. The results from this study illuminate fascinating aspects of African American history and the complex context that frames some African American women's choice about breastfeeding versus artificial supplementation feeding. This study also demonstrates the need for developing family centered and culturally relevant strategies to increase the African American breastfeeding rate.

Renal function of cyclin M2 Mg2+ transporter maintains blood pressure.

Epidemiological studies have shown that magnesium intake/excretion is inversely correlated with blood pressure (BP), and artificial supplementation of magnesium was able to prevent hypertension. However, there has been no molecular genetic study showing the importance of magnesium homeostasis in BP regulation. We analyzed magnesium content and BP of mice lacking genes encoding cyclin M (CNNM) Mg transporter family proteins. Systemic heterozygotes and the kidney-specific homozygotes for Cnnm2-deficient alleles are both viable and show hypomagnesemia, indicating the important function of CNNM2 in maintaining magnesium homeostasis in the kidney. Endogenous CNNM2 localizes at the basolateral membrane of kidney distal convoluted tubule cells, which play important roles not only in magnesium reabsorption but also in BP control. The BP of these viable strains is significantly reduced; the SBP values by telemetric measurements are 121.7 ± 2.8 mmHg in wild-type, and 110.2 ± 2.7 and 109.7 ± 3.6 mmHg in systemic heterozygotes and kidney-specific homozygotes, respectively. Analyses of mice lacking CNNM Mg transporters clearly demonstrated abnormalities in BP values, confirming the importance of magnesium homeostasis in maintaining BP.