Artemisia Princeps Var Research Articles

Artemisia Extract Suppresses NLRP3 and AIM2 Inflammasome Activation by Inhibition of ASC Phosphorylation.

Artemisia princeps var. orientalis (Asteraceae, A. princeps) is a well-known traditional medicinal herb used for treating various inflammatory disorders in Korea, Japan, China, and other Asian countries. In the present study, we investigated the effects of A. princeps extract (APO) on interleukin- (IL-) 1β regulation and inflammasome activation in bone marrow-derived macrophages (BMDMs) and monosodium urate- (MSU-) induced peritonitis mouse model in vivo. The APO treatment to BMDMs primed with lipopolysaccharide (LPS) attenuated the NLRP3 and AIM2 inflammasome activation induced by danger signals, such as ATP, nigericin, silica crystals, and poly (dA:dT), respectively. Mechanistic study revealed that APO suppressed the ASC oligomerization and speck formation, which are required for inflammasome activation. APO treatment also reduced the ASC phosphorylation induced by the combination of LPS and a tyrosine phosphatase inhibitor. In vivo evaluation revealed that intraperitoneal administration of APO reduced IL-1β levels, significantly (p < 0.05) and dose dependently, in the MSU-induced peritonitis mouse model. In conclusion, our study is the first to report that the extract of A. princeps inhibits inflammasome activation through the modulation of ASC phosphorylation. Therefore, APO might be developed as therapeutic potential in the treatment of inflammasome-mediated inflammatory disorders, such as gouty arthritis.

Antiviral activities of Artemisia princeps var. orientalis essential oil and its α-thujone against norovirus surrogates.

Artemisia princeps var. orientalis is a well-known medicinal food, which has been used for the treatment of several diseases including bacterial infection. We examined the antiviral effects of the essential oil from A. princeps var. orientalis and its compounds, borneol, α-thujone and camphor, against murine norovirus-1 (MNV-1) and feline calicivirus-F9 (FCV-F9). The time-of-addition plaque assays were used to determine the ability of essential oil to interfere with viral infection. The maximum activities, following the pretreatment of FCV-F9 and MNV-1, reached 48% inhibition on FCV-F9 and 64% inhibition on MNV-1 at 0.1 and 0.01% of the essential oil, respectively. Neither borneol nor camphor exhibited an antiviral activity, whereas α-thujone, a major compound of the essential oil, showed strong inhibition on FCV-F9 and MNV-1.

The Variation of the Major Compounds of Artemisia princeps var. orientalis (Pampan) Hara Essential Oil by Harvest Year

The Variation of the Major Compounds of Artemisia princeps var. orientalis (Pampan) Hara Essential Oil by Harvest Year

Erratum to: Characterization of secondary metabolite compounds correlated with the seasons in Artemisia princeps var. orientalis (Pamp.) H. Hara leaves using direct sample injection and gas chromatography–mass spectrometry: contribution to phytotoxicity

Erratum to: Characterization of secondary metabolite compounds correlated with the seasons in Artemisia princeps var. orientalis (Pamp.) H. Hara leaves using direct sample injection and gas chromatography–mass spectrometry: contribution to phytotoxicity

Characterization of secondary metabolite compounds correlated with the seasons in Artemisia princeps var. orientalis (Pamp.) H. Hara leaves using direct sample injection and gas chromatography–mass spectrometry: contribution to phytotoxicity

Leaves from a natural population of Artemisia princeps var. orientalis (Pamp.) H. Hara were collected monthly from April through October and characterized for composition of secondary metabolite compounds and their phytotoxic effects on seed germination and seedling growth of Achyranthes japonica and Lactuca sativa. The compounds were identified using gas chromatography/mass spectrometry (GC/MS) coupled with a solvent-free solid injector (SFSI). GC/MS analyses of all samples revealed qualitative variability in the composition of secondary metabolites. The greatest number of compounds was identified in July (56) followed by September (30) and April (24), and the lowest number was found in June (2) and August (2). Among 92 compounds, the major compounds were various terpenes (23) (mono-, sesqui, di-, and tri-terpenes) followed by heterocyclic compounds (18) and hydrocarbons (14). The higher the concentration of the secondary metabolites, the lower the seed germination and seedling growth of A. japonica and L. sativa. Plant samples collected in July and August were most detrimental. Taken together, variability in the secondary metabolites compounds of A. princeps var. orientalis was verified during different seasons, and the compounds were successfully identified by a combination of SFSI and GC/MS. Notably, the antimicrobial and antioxidative effects were inconsistent throughout the various seasons.

Comparison of Anti-oxidative and UV Absorption Abilities of Methanolic Extracts from Leaves, Stem, and Roots of Artemisia princeps var. orientalis

Comparison of Anti-oxidative and UV Absorption Abilities of Methanolic Extracts from Leaves, Stem, and Roots of Artemisia princeps var. orientalis

HaCaT 세포에서 자외선 B에 의해 유도된 DNA 상해반응에 대한 복분자와 쑥 추출물의 효과

본 연구는 복분자와 쑥 추출물이 UVB (자외선-B)에 의한 유전독성의 감소에 효과를 보이는 지를 HaCaT 세포를 이용하여 분석하였다. 자외선을 조사하지 않은 정상 세포의 세포활성은 복분자와 쑥 추출물을 처리한 경우 처리하지 않은 대조군과 차이를 나타내지 않았지만, 자외선을 조사한 세포에 복분자와 쑥 추출물을 처리한 경우 처리하지 않은 대조군에 비해 농도 의존적으로 세포 활성을 증가하였다. UVB를 조사한 후 세포의 핵 분절율은 복분자와 쑥 추출물을 처리한 경우 정상 배양액으로 배양한 대조군에 비해 핵 분절율을 각각 약 20%, 15% 정도 감소하였다. DNA 상해 회복에 반응하는 cyclobutane pyrimidine dimer (CPD)의 잔여량을 확인한 결과, 복분자와 쑥 추출만 처리한 경우 처리하지 않은 대조군에 비해 차이를 나타내지 않았으나 UVB 조사한 후 복분자와 쑥추출물을 처리한 경우 정상 배양액으로 배양한 대조군에 비해 농도의존적으로 감소하였다. 세포 상해에 반응하는 유전자인 phospho-p53과 GADD45의 단백질 수준을 Western blot으로 분석한 결과, 자외선을 조사하지 않은 정상세포에 복분자와 쑥 추출물만 처리한 경우 처리하지 않은 대조군과 비교하여 유의적인 차이를 나타내지 않았지만 UVB 조사한 후 복분자와 쑥 추출물을 처리한 경우 농도 의존적으로 phospho-p53과 GADD45 단백질 발현량이 감소하였다. 위의 결과에서 복분자와 쑥 추출물은 UVB에 유도된 DNA 상해와 p53 및 GADD45와 같은 상해 반응단백질의 수준을 감소시키는 것으로 보아 UVB에 의해 손상된 세포의 유전독성을 회복하는 효능이 있다고 생각된다. We investigated the effects of extracts from Rubus coreanus (RC) and Artemisia princeps var. orientalis (AP) on DNA damage response in ultraviolet B (UVB)-exposed HaCaT cells. Cell activity upon treatment for 24 h with RC or AP alone was similar to or greater than that of the nontreated control. When UVB-exposed cells were postincubated for 24 h in medium containing RC or AP, cell activity increased in a concentration-dependent manner. Nuclear fragmentation analysis showed that postincubation with RC or AP decreased UVB-induced apoptosis by about 20% and 15%, respectively, of that in cells postincubated with growth medium. When UVB-exposed cells were postincubated for 24 h in medium containing RC or AP, the level of cyclobutane pyrimidine dimer decreased in a concentration- dependent manner. Western blot analysis showed that treatment of cells not exposed to UVB with RC or AP alone did not significantly change the levels of phospho-p53 and GADD45 protein. Interestingly, when UVB-exposed cells were postincubated for 24 h in medium containing RC or AP, phospho-p53 and GADD45 levels decreased in a concentration dependent manner. Our results suggest that RC and AP extract assist the survival of UVB-exposed cells in parallel with a decrease in levels of UVB-induced DNA damage and damage-response proteins, such as p53 and GADD45.

고속도로 비탈면의 식생천이 특성 연구 I

This study was carried out in order to analyze the succession characteristics of the slope of expressways and the results of analysis by collecting and analyzing various literatures and data from 2011 to 2012 and surveying 75 expressway slope, as follows : The construction methods applied to planting the slope of an expressway can be classified into 4 types including 3 Climber planting methods, 8 Vegetation thick-layer spray work methods, 4 Seed spray work methods, and 4 Stabilization work methods. The factors which affect the cover degree of the slope of an expressway were found to be development years, gradient, length, and azimuth. Like surrounding forest areas, the expressway slope was analyzed to begin the plant succession 20, 30, and 40 years after development, and plant succession was developed in diversity in a mixed stand forest according to surrounding forest floors. Species diversity, maximum species diversity, and the evenness of slope facing north were analyzed to be high comparing to those facing east, west, and south according to azimuth of slope. Species diversity, maximum species diversity, evenness of slope, and the plant succession of surroundings were analyzed to be high when the gradient of the slope was less than . The dominant species which success on the slope of an expressway and its surrounding forest area included Miscanthus sinensis var. purpurascens, Zoysia japonica, Pinus thunbergii Parl, Rubus crataegifolius, Lespedeza cyrtobotrya, Amorpha fruticosa, Artemisia princeps var. orientalis, and Oenothera biennis.

Artemisia princeps var. orientalis induces cell cycle arrest accompanied by upregulation of p21CIP1 and caspase‐dependent apoptosis in human breast carcinoma cells

The aim of the present study was to investigate the anticancer activity and mechanism of action of methanol extract from Artemisia princeps var. orientalis (APME). First, the antiproliferative activity of APME against T47D cells was at various concentrations (5–200 μg/mL) and treatment times (24–72 h) determined by MTT assay. APME significantly inhibited cell proliferation in a dose‐dependent manner, regardless of treatment times. Under the same condition, cytotoxicity (LDH release) of APME was only appeared at 72 h. In addition, treatment with the IC50 concentration of APME for 24 h caused cell cycle arrest and apoptosis. APME arrested cells in both G1 and G2/M phases of the cell cycle, a response that was related to the induction of CDK1, cyclin B, p21Cip1 expression, and repression of CDK2 and cyclin A. The induction of p21Cip1 by APME caused activation of p53 and modulation of its downstream targets Bcl‐2 and Bax. Subsequently, caspase‐8 and ‐9 were cleaved in cells treated with APME. Activation of these caspases in turn led to increased activation of caspase‐3. The results of this study suggest that APME produce anticancer effects in T47D cells through cell cycle arrest associated with the induction of p21Cip1 and apoptosis mediated by p53 and caspase‐depedent pathways.This research was supported by Basic Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2012–0006811)

An Evaluation of Plant Growth Promoting Activities and Salt Tolerance of Rhizobacteria Isolated from Plants Native to Coastal Sand Dunes

Coastal sand dunes are important for ecosystems due to the variety of rare species that can be found in this kind of habitat, and the beautiful landscapes they create. For environmental remediation, a potential strategy is phytoremediation using the symbiotic relationship of plants and microbes in the rhizosphere, which has proven ecologically sound, safe, and cost effective. Ninety-five colonies were isolated from the rhizosphere soil (RS) or rhizoplane (RP) of Rorippa islandica, Rumex crispus, Artemisia princeps var. orientalis, Lilium sp Stellaria media, and Gramineae. These colonies were then tested for plant growth promoting activities (PGPAs) such as 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, and siderphores synthesis ability. In addition, salt tolerance was evaluated at 4% and 8% salt concentrations. It was observed that amongst the test subjects about 50% of the strains had a high resistance to salinity. Many of them could produce indole-3-acetic acid (IAA) IAA (in RS 13.9% and in RP 7.6%), exhibited ACC deaminase activity (55.8% in RS and 36.6% in RP), and could synthesize siderphores (62.7% in RS and 50% in RP). Correlation coefficient analyses were carried out for the three kinds of plant growth promoting abilities (PGPA) and salt tolerance. A positive correlation was found between an ability to synthesize siderphores and ACC deaminase activity (r=0.605, p

Effects of Plant Extracts on Microbial Population, Methane Emission and Ruminal Fermentation Characteristics in In vitro.

This study was conducted to evaluate effects of plant extracts on methanogenesis and rumen microbial diversity in in vitro. Plant extracts (Artemisia princeps var. Orientalis; Wormwood, Allium sativum for. Pekinense; Garlic, Allium cepa; Onion, Zingiber officinale; Ginger, Citrus unshiu; Mandarin orange, Lonicera japonica; Honeysuckle) were obtained from the Plant Extract Bank at Korea Research Institute of Bioscience and Biotechnology. The rumen fluid was collected before morning feeding from a fistulated Holstein cow fed timothy and commercial concentrate (TDN; 73.5%, crude protein; 19%, crude fat; 3%, crude fiber; 12%, crude ash; 10%, Ca; 0.8%, P; 1.2%) in the ratio of 3 to 2. The 30 ml of mixture, comprising McDougall buffer and rumen liquor in the ratio of 4 to 1, was dispensed anaerobically into serum bottles containing 0.3 g of timothy substrate and plant extracts (1% of total volume, respectively) filled with O2-free N2 gas and capped with a rubber stopper. The serum bottles were held in a shaking incubator at 39°C for 24 h. Total gas production in all plant extracts was higher (p<0.05) than that of the control, and total gas production of ginger extract was highest (p<0.05). The methane emission was highest (p<0.05) at control, but lowest (p<0.05) at garlic extract which was reduced to about 20% of methane emission (40.2 vs 32.5 ml/g DM). Other plant extracts also resulted in a decrease in methane emissions (wormwood; 8%, onion; 16%, ginger; 16.7%, mandarin orange; 12%, honeysuckle; 12.2%). Total VFAs concentration and pH were not influenced by the addition of plant extracts. Acetate to propionate ratios from garlic and ginger extracts addition samples were lower (p<0.05, 3.36 and 3.38 vs 3.53) than that of the control. Real-time PCR indicted that the ciliate-associated methanogen population in all added plant extracts decreased more than that of the control, while the fibrolytic bacteria population increased. In particular, the F. succinogens community in added wormwood, garlic, mandarin orange and honeysuckle extracts increased more than that of the others. The addition of onion extract increased R. albus diversity, while other extracts did not influence the R. albus community. The R. flavefaciens population in added wormwood and garlic extracts decreased, while other extracts increased its abundance compared to the control. In conclusion, the results indicated that the plant extracts used in the experiment could be promising feed additives to decrease methane gas emission from ruminant animals while improving ruminal fermentation.

혼합물 실험계획법에 의한 쑥 첨가 찜 케이크의 최적화

This study examined the optimal ingredient mixing ratio for the preparation of steamed cake containing mugwort (Artemisia princeps var. orientalis) powder. After preliminary studies, the following ingredient ranges were determined; 110~129% sugar, 3~8% mugwort powder, 10~25% oil. Among the different mixture designs, the D-optimal design was chosen for analysis. The results of F-test, specific gravity, viscosity, volume and color values (L, a, b), hardness decided a linear model, while the sensory characteristics (color, taste, texture and overall acceptance) decided a quadratic model. The fitness analysis results showed that in all characteristic, the probabilities were significant within 0.05%; thus, the models were accepted as appropriate. The response surface and trace plot results showed that increasing amounts of mugwort powder decreased the brightness, and increased redness and yellowness. As the level of added oil increased, the softness of the cake increased. Cake samples received low sensory evaluation scores when sugar, mugwort powder, and oil were added above their optimal levels. The optimum formulations by the numerical and graphical methods, were similar, and with the numerical method presented as: sugar, mugwort powder, and oil at 120.7%, 5.1%, and 16.2%, respectively(flour weight basis). The above results demonstrate the feasibility of adding mugwort powder to sponge cake, and therefore, the commercialization of mugwort powder cake marketed as a functional food is deemed possible.

The Memory-Ameliorating Effects of Artemisia princeps var. orientalis Against Cholinergic Dysfunction in Mice

Artemisia princeps var. orientalis (Compositae) is widely distributed in China, Japan and Korea and is known to have anti-inflammatory and anti-oxidative activities. The ethyl acetate fraction of ethanolic extract of A. princeps var. orientalis (AEA) was found to inhibit acetylcholinesterase activity in a dose-dependent manner in vitro (IC(50) value: 541.4 ± 67.5 μg/ml). Therefore, we investigated the effects of AEA on scopolamine-induced learning and memory impairment using the passive avoidance, the Y-maze, and the Morris water maze tasks in mice. AEA (100 or 200 mg/kg, p.o.) significantly ameliorated scopolamine-induced cognitive impairments in the passive avoidance and Y-maze tasks (p < 0.05). In the Morris water maze task, AEA (200 mg/kg, p.o.) significantly shortened escape latencies in training trials and increased both swimming time spent in the target zone and probe crossing numbers during the probe trial as compared with scopolamine-treated mice (p < 0.05). Additionally, the ameliorating effect of AEA on scopolamine-induced memory impairment was antagonized by a subeffective dose of MK-801. These results suggest that AEA could be an effective treatment against cholinergic dysfunction and its effect is mediated by the enhancement of the cholinergic neurotransmitter system via NMDA receptor signaling or acetylcholinesterase inhibition.

Isolation and quantitative analysis of peroxynitrite scavengers from Artemisia princeps var. orientalis

Young and mature Artemisia princeps var. orientalis (APO, Compositae) are used as a health food and a medicinal plant, respectively, in Korea. Here, we identified the in vitro potent peroxynitrite (ONOO(-))-scavenging effect (IC(50), 0.26 microg/mL) of the components from the EtOAc fraction. Octadecylsilane column chromatography on the EtOAc fraction yielded two caffeoylquinic acid compounds, 3,5-di-O-caffeoyl-muco-quinic acid (1) and methyl 4,5-di-O-caffeoylquinate (2) by NMR spectroscopic data, which have not been reported before from APO. The IC(50) values of compounds 1 and 2 were 0.18 +/- 0.01 microg/mL and 0.12 +/- 0.00 microg/mL, respectively, lower than that of the positive control (L-penicillamine). HPLC data indicated that young APO (1: 30.3 mg/g dried weight, 2: 27.7 mg/g) contained considerably higher quantities of the two caffeoylquinic acids than mature APO (1: 1.77 mg/g dried weight, 2: 4.10 mg/g).

금강 유역의 수변 식생 현황과 분포

Riparian vegetation gives basic habitats for animal and other biological organisms. It is one of essential landscapes in river ecosystem. This floral study was conducted to give basic information for management of the Geum river ecosystem. We discovered total 69 plant communities out of 40 sites studied and mean 8 plant communities were analysed per study site. Salix koreensis and Salix subfragilis community were dominant of all communities at tree layer and Salix gracilistyla and Rubus parvifolius were dominant communities at shrub layer. Phalaris arundinacea, Phragmites japonica, Miscanthus sacchariflorus and Artemisia princeps var. orientalis appeared most as perennial grasses and Humulus japonicus, Persicaria thunbergii and Persicaria hydropiper appeared most as annual grasses. Robinia pseudoacacia, Amorpha fruticosa and Bidens frondosa were frequently present as exotic species. Grass communities were higher than tree communities because of human disturbance by artificial banks. The number of species was 182 appeared as 53 families 144 species, 34 varieties and 4 forma and mean species number was 25.5 per site. The most abundant site was Yanggang bridge site (14 vegetation association, 49 species) and the least abundant site was Namdaechon, Muju site (3 vegetation association, 12 species). We suggest that continuous riparian vegetation monitoring should be carried out to manage water environment ecologically.

The influence of the growth season on the antimicrobial and antioxidative activity in Artemisia princeps var. orientalis

We investigated the influence of the growth season of Artemisia princeps var. orientalis (P AMPAN.) H ARA on antimicrobial and antioxidative activities. The ethyl acetate and water fractions of crude methanol extract from the plant collected monthly (April through October) were tested against three gram-positive, two gram-negative bacteria and one lactic acid bacterium. The ethyl acetate and water fractions of crude methanol extract from A. princeps var. orientalis collected in August and September had the highest antimicrobial activity. The antimicrobial activity of extract from A. princeps var. orientalis for Lactobacillus plantarum was not found at any concentration and in any growth season. Minimum inhibitory concentration (MIC) for each strain by ethyl acetate fraction of methanol extract from A. princeps var. orientalis was different depending on the tested strain and growth season of the plant. MIC for each strain was the highest in the sample from April, followed by October. The antioxidative activities of methanol extracts from A. princeps var. orientalis collected monthly were measured by their scavenging activity on free radical of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and xanthine oxidase (XO) inhibitory activity. The DPPH radical scavenging activities of the extracts from the plants collected in May, June and July were higher than any other. The XO inhibitory activities of the extracts of A. princeps var. orientalis collected in April and May showed the greatest activity, it is different with antimicrobial activity. This is the first report that antimicrobial and antioxidative activity of the extracts of A. princeps var. orientalis is different with growth season.

Enhancement of Cadmium Phytoextraction from Contaminated Soils with Artemisia princeps var. orientalis

Enhancement of Cadmium Phytoextraction from Contaminated Soils with Artemisia princeps var. orientalis

Distribution of Heavy Metal Content in Plants and Soil from a Korean Shooting Site

In this research we determined the levels of heavy metals in soil and metal-accumulating plants from a D military shooting site in the Kyungkido district of Korea. The data obtained may be useful in the development of methods for the efficient phytoremediation of contaminated soil. The total Cd, Cu, Pb, and Zn concentrations in the soil were found to be 1.67-5.04 mg/kg, 52.51-106.26 mg/kg, 37.24-90.32mg/kg, and 111.45-188.19mg/kg, respectively. These results show that the soil is contaminated with Cd and Cu, and this contamination is particularly severe in the case of Cd because of its high bioavailability (25-57% of the total metal in the soil is exchangeable). The high concentrations of heavy metals in the shoots of Persicaria thunbergii and Artemisia princeps var. orientalis indicate that these plants (all perennial herbs) accumulate heavy metal efficiently. Further, these plants were found to contain more Cd in its shoots (＞60% of the total metal found in the plant) than any other plant; these results indicate that these native species are particularly suited to use in Cd phytoextraction.

Antioxidant Activity of Medicinal Plant Extracts Used as Folk Remedies by Diabetic Patients

The aim of this study was to investigate the antioxidant effect of medicinal plants used by diabetic patients. Fourteen medicinal plants were selected and antioxidant activity such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, inhibition effect of linoleic acid autoxidation and low density lipoprotein (LDL) oxidation, thiobarbituric acid (TBA) value were measured. The Cornus officinalis had DPPH radical scavenging activity of 84.79%, which was higher than the 78.79% for α-tocopherol. Rosa rugosa Thunberg, Pueraria thundergiana Bentham, Artemisia princeps var. orientalis and Sasamorpha purpurascens Nakai also had high values. Extracts with higher DPPH radical scavenging activities had higher total phenol concentrations, and positive correlations between these parameters were found. Inhibitory activities of linoleic acid autoxidation, LDL oxidation and TBA value, used as indices of oxidative stress, were observed in most of the selected medicinal plants. The highest inhibitory activity for TBA value was observed in the extract of Pueraria thundergiana Bentham. Rosa rugosa Thunberg (75.50%), Sasamorpha purpurascens Nakai (74.00%), and Cornus officinalis (73.00%) all had high antioxidant activity against linoleic acid autoxidation, similar to that of α-tocopherol. The same 3 plants also had high in inhibitory activity against LDL oxidation. Therefore, we demonstrated that medicinal plants used as folk remedy by diabetic patients had antioxidant activity. Especially, Rosa rugosa Thunberg, Cornus officinalis, Pueraria thundergiana Bentham, Artemisia princeps var. orientalis, and Sasamorpha purpurascens Nakai had high phenol concentrations which resulted in high values for DPPH radical scavenging. These same plants exhibited high values for inhibitory activities for TBA value, linoleic acid autoxidation and LDL oxidation, indices associated with lipid peroxidation.

경기도 오산천 주변의 잡초군락에 대한 식물사회학적 연구

오산천은 경기도 남부지역에 위치하는 대표적인 도시 하천이다. 경기도 수원시를 비롯하여 화성군, 오산시 등의 도시 및 농촌, 공단이 인접하고 있어 하천에 대한 인위적, 자연적인 교란이 매우 빈번하게 나타나는 하천이다. 오산천 주변에 형성된 잡초 군락의 유형 및 실태를 파악하여 금후 자연형 하천으로의 복원 및 관리에 기초 자료로 활용하기 위해 본 연구를 수행하였다 따라서 하천 주변의 수변부, 고수부, 제방부 등에 형성된 잡초 군락에 대해 2003년 5월에서 2003년 9월까지 식물사회학적인 방법에 의해 조사를 수행하여 군락을 유형화하고 그 환경 조건을 분석하였다. 오산천 주변의 잡초 군락은 8군락 단위로 구분되었다: A. 환삼덩굴 군락, B. 갈대군락, C. 고마리 군락, D. 줄 군락, E. 물억새 군락, F. 돌피 군락, G. 부들 군락, H. 강아지풀 군락. 이상의 8군락을 구성하는 총 식물의 종류는 19과 36속 4변종 40종의 총 44분류군으로 조사되었다. 각 군락의 수반종으로 주요 출현 식물은 쑥, 개망초, 자귀풀, 달맞이꽃, 방동사니, 미국가막사리 등 주로 호광성이며 환경압에 강한 식물들로 나타났다. 모든 조사구를 Bray-Curtis 서열법에 의해 서열화한 결과는 대체로 식물사회학적 표 조작에서 얻어진 결과와 유사한 경향으로 나타났다. Osancheon creek is located in the southwest of the middle area in Korea. Weed community around the Osancheon creek, where various disturbances exert a bad influence directly on its community by human work or nature, was surveyed phytosocialogically. Our surveys have been accomplished from June, 2003 to September, 2003. Weed communities formed around the Osancheon creek were divided into several patterns and analysed. They have been divided into 8 communities. Community A: Humulus japonica community, B: Phragmites communiscommunity, C: Persicaria thunbergii community, D: Zi=ania latifolia community, E: Miscanthus saccharilflorus community, F: Echinochica crusgalli community, G: Typha orientalis community, H: Sataria viridis community. The flora surveyed in these communities was constituted of 19 families, 36 genera, 4 varieties, and 40 species. Wild plants such as Artemisia princeps var. orientalis Erigeron annuus, Bidens frondosa, Aeschynomene indica and Oenothera odorata were mostly light loving plants and higher resistant plants against the disturbance. Our result from the ranking all surveyed areas by the Bray-Curtis ordination method was very similar to the results from phytosocialogical table work.