Arsenite-oxidizing Bacteria Research Articles

A Sb(III)-specific efflux transporter from Ensifer adhaerens E-60

Antimony is pervasive environmental toxic substance, and numerous genes encoding mechanisms to resist, transform and extrude the toxic metalloid antimony have been discovered in various microorganisms. Here we identified a major facilitator superfamily (MFS) transporter, AntB, on the chromosome of the arsenite-oxidizing bacterium Ensifer adhaerens E-60 that confers resistance to Sb(III) and Sb(V). The antB gene is adjacent to gene encoding a LysR family transcriptional regulator termed LysRars, which is an As(III)/Sb(III)-responsive transcriptional repressor that is predicted to control expression of antB. Similar antB and lysRars genes are found in related arsenic-resistant bacteria, especially strains of Ensifer adhaerens, and the lysRars gene adjacent to antB encodes a member of a divergent subgroup of putative LysR-type regulators. Closely related AntB and LysRars orthologs contain three conserved cysteine residues, which are Cys17, Cys99, and Cys350 in AntB and Cys81, Cys289 and Cys294 in LysRars, respectively. Expression of antB is induced by As(III), Sb(III), Sb(V) and Rox(III) (4-hydroxy-3-nitrophenyl arsenite). Heterologous expression of antB in E. coli AW3110 (Δars) conferred resistance to Sb(III) and Sb(V) and reduced the intracellular concentration of Sb(III). The discovery of the Sb(III) efflux transporter AntB enriches our knowledge of the role of the efflux transporter in the antimony biogeochemical cycle.

Genome sequence and characterisation of a freshwater photoarsenotroph, Cereibacter azotoformans strain ORIO, isolated from sediments capable of cyclic light-dark arsenic oxidation and reduction.

A freshwater photosynthetic arsenite-oxidizing bacterium, Cereibacter azotoformans strain ORIO, was isolated from Owens River, CA, USA. The waters from Owens River are elevated in arsenic and serve as the headwaters to the Los Angeles Aqueduct. The complete genome sequence of strain ORIO is 4.8 Mb genome (68% G + C content) and comprises two chromosomes and six plasmids. Taxonomic analysis placed ORIO within the Cereibacter genus (formerly Rhodobacter). The ORIO genome contains arxB2 AB1 CD (encoding an arsenite oxidase), arxXSR (regulators) and several ars arsenic resistance genes all co-localised on a 136 kb plasmid, named pORIO3. Phylogenetic analysis of ArxA, the molybdenum-containing arsenite oxidase catalytic subunit, demonstrated photoarsenotrophy is likely to occur within members of the Alphaproteobacteria. ORIO is a mixotroph, oxidises arsenite to arsenate (As(V)) photoheterotrophically, and expresses arxA in cultures grown with arsenite. Further ecophysiology studies with Owens River sediment demonstrated the interconversion of arsenite and As(V) was dependent on light-dark cycling. arxA and arrA (As(V) respiratory reductase) genes were detected in the light-dark cycled sediment metagenomes suggesting syntrophic interactions among arsenotrophs. This work establishes C. azotoformans str. ORIO as a new model organism for studying photoarsenotrophy and light-dark arsenic biogeochemical cycling.

Different sulfide to arsenic ratios driving arsenic speciation and microbial community interactions in two alkaline hot springs

Arsenic (As) is ubiquitous in geothermal fluids, which threatens both water supply safety and local ecology. The co-occurrence of sulfur (S) and As increases the complexity of As migration and transformation in hot springs. Microorganisms play important roles in As–S transformation processes. In the present study, two Tibetan alkaline hot springs (designated Gulu [GL] and Daba [DB]) with different total As concentrations (0.88 mg/L and 12.42 mg/L, respectively) and different sulfide/As ratios (3.97 and 0.008, respectively) were selected for investigating interactions between As–S geochemistry and microbial communities along the outflow channels. The results showed that As–S transformation processes were similar, although concentrations and percentages of As and S species differed between the two hot springs. Thioarsenates were detected at the vents of the hot springs (18% and 0.32%, respectively), and were desulfurized to arsenite along the drainage channel. Arsenite was finally oxidized to arsenate (532 μg/L and 12,700 μg/L, respectively). Monothioarsenate, total As, and sulfate were the key factors shaping the changes in microbial communities with geochemical gradients. The relative abundances of sulfur reduction genes (dsrAB) and arsenate reduction genes (arsC) were higher in upstream portions of GL explaining high thiolation. Arsenite oxidation genes (aoxAB) were relatively abundant in downstream parts of GL and at the vent of DB explaining low thiolation. Sulfur oxidation genes (soxABXYZ) were abundant in GL and DB. Putative sulfate-reducing bacteria (SRB), such as Desulfuromusa and Clostridium, might be involved in forming thioarsenates by producing reduced S for chemical reactions with arsenite. Sulfur-oxidizing bacteria (SOB), such as Elioraea, Pseudoxanthomonas and Pseudomonas, and arsenite-oxidizing bacteria (AsOB) such as Thermus, Sulfurihydrogenibium and Hydrogenophaga, may be responsible for the oxidation of As-bound S, thereby desulfurizing thioarsenates, forming arsenite and, by further abiotic or microbial oxidation, arsenate. This study improves our understanding of As and S biogeochemistry in hot springs.

Whole-Genome Sequence of an Arsenite-Oxidizing Bacterium, Pandoraea sp. Strain NE5, Isolated from the Rhizosphere of the Arsenic Hyperaccumulator Pteris vittata.

Pandoraea sp. strain NE5, an arsenite-oxidizing bacterium, was isolated from the rhizosphere of an arsenic hyperaccumulator fern (Pteris vittate). Here, the genome sequence of Pandoraea sp. strain NE5 is announced.

Ionome profiling and arsenic speciation provide evidence of arsenite detoxification in rice by phosphate and arsenite-oxidizing bacteria

Arsenite (As(III)) as the most toxic and mobile form is the dominant arsenic (As) species in flooded paddy fields, resulting in higher accumulation of As in paddy rice than other terrestrial crops. Mitigation of As toxicity to rice plant is an important way to safeguard food production and safety. In the current study, As(III)-oxidizing bacteria Pseudomonas sp. strain SMS11 was inoculated with rice plants to accelerate conversion of As(III) into lower toxic arsenate (As(V)). Meanwhile, additional phosphate was supplemented to restrict As(V) uptake by the rice plants. Growth of rice plant was significantly inhibited under As(III) stress. The inhibition was alleviated by the introduction of additional P and SMS11. Arsenic speciation showed that additional P restricted As accumulation in the rice roots via competing common uptake pathways, while inoculation with SMS11 limited As translocation from root to shoot. Ionomic profiling revealed specific characteristics of the rice tissue samples from different treatment groups. Compared to the roots, ionomes of the rice shoots were more sensitive to environmental perturbations. Both extraneous P and As(III)-oxidizing bacteria SMS11 could alleviate As(III) stress to the rice plants through promoting growth and regulating ionome homeostasis.

Understanding the slight inhibition of high As(III) stress on nitritation process: Insights from arsenic speciation and microbial community analyses

Nitritation process with ammonia-oxidizing bacteria frequently suffers inhibition from heavy metals in industrial wastewater treatment. However, As(III), one of the most toxic metalloids, showed slight inhibition though the arsenic accumulation content in the sludge reached 91.8 mg L−1 in this study. Here, we combined long-term reactor operation with microbiological analyses to explore the slight inhibition mechanisms of As(III) on nitritation consortia. The results showed that no obvious changes induced by As(III) occurred in apparent characteristics and morphology of the nitritation consortia, whereas dosing As(III) induced shifts in the arsenic speciation and microbial community. 84.1% of As(III) was oxidized to As(V) in the acclimated sludge, decreasing the toxicity of As(III) to nitritation consortia. Insight to the microbial community, the relative abundances of Thermaceae and Phycisphaeraceae responsible for As(III) oxidation were increased to 7.4% and 6.6% under the stress of high-concentration As(III), respectively. Further, these increased arsenite-oxidizing bacteria probably accepted electron acceptor NO2- from ammonia-oxidizing bacteria to oxidize As(III). Our results indicated that microbial As(III) oxidation was the dominant detoxification pathway, providing new insights into nitritation characteristics under long-term As(III) stress.

Implementation of Biotechnological Techniques in Treatment of Groundwater Contaminated with Arsenic

Abstract: Over the course of time, there seemed to be an increase in rate of Arsenic (As) contamination in groundwater found around the world. Several organic or/and anthropogenic resources have emerged to be the primary cause in contributing to a negative impact on public health as well as on the environment. A large number of people throughout the world rely on groundwater containing toxic levels of As for their drinking needs. When As is exposed to drinking water in a significant amount for a great period of time, it may lead to several disabling and weakening diseases. the most common one being a dermatological condition known as Arsenicosis. The sources, distribution, migration of As, as well as a worldwide summary of its contamination in drinking water are discussed in this paper. The research also examines the human health hazards associated with As, as well as its absorption process and contribution in the poisonous pathways alongside providing an introduction of modern evidence on treating groundwater with several Biological/Biotechnological techniquesas a substitute or potential replacement of the existing and well known physical-chemical methods used in the purification process. Keywords: Arsenic, Biological Iron Oxidation,Arsenite Oxidizing Bacterium;

Bioaccumulation and detoxification of trivalent arsenic by Achromobacter xylosoxidans BHW-15 and electrochemical detection of its transformation efficiency

Arsenotrophic bacteria play an essential role in lowering arsenic contamination by converting toxic arsenite [As (III)] to less toxic and less bio-accumulative arsenate [As (V)]. The current study focused on the qualitative and electrocatalytic detection of the arsenite oxidation potential of an arsenite-oxidizing bacteria A. xylosoxidans BHW-15 (retrieved from As-contaminated tube well water), which could significantly contribute to arsenic detoxification, accumulation, and immobilization while also providing a scientific foundation for future electrochemical sensor development. The minimum inhibitory concentration (MIC) value for the bacteria was 15 mM As (III). Scanning Electron Microscopy (SEM) investigation validated its intracellular As uptake capacity and demonstrated a substantial association with the MIC value. During the stationary phase, the strain’s As (III) transformation efficiency was 0.0224 mM/h. Molecular analysis by real-time qPCR showed arsenite oxidase (aioA) gene expression increased 1.6-fold in the presence of As (III) compared to the untreated cells. The immobilized whole-cell also showed As (III) conversion up to 18 days. To analyze the electrochemical oxidation in water, we developed a modified GCE/P-Arg/ErGO-AuNPs electrode, which successfully sensed and quantified conversion of As (III) into As (V) by accepting electrons; implying a functional As oxidase enzyme activity in the cells. To the best of our knowledge, this is the first report on the electrochemical observation of the As-transformation mechanism with Achromobactersp. Furthermore, the current work highlighted that our isolate might be employed as a promising candidate for arsenic bioremediation, and information acquired from this study may be helpful to open a new window for the development of a cost-effective, eco-friendly biosensor for arsenic species detection in the future.

Arsenite-oxidizing bacteria isolated from an abandoned realgar mining area: Characterization and the influence on arsenic accumulation in rice seedlings

Rice consumption is the main route of human exposure to arsenic (As) contamination. Oxidation of more toxic arsenite [As(III)] to less toxic arsenate [As(V)] is considered as a potential strategy to alleviate As contamination in rice plant. In this study, two As(III)-oxidizing bacterial strains SMS06 and SMS11 were isolated from the As-contaminated soils collected from an abandoned realgar mining area in Shimen County, China. According to the 16S rRNA gene sequences, SMS06 and SMS11 were affiliated with genera Achromobacter and Pseudomonas, respectively. The aioA gene was amplified in both strains while the arsB and arsM genes were not detected, indicating that the detoxification of As(III) in the strains was mainly through oxidation process governed by the aioA gene. Under aerobic condition, strains SMS06 and SMS11 could completely oxidize 600 and 450 mg/L of As(III) to As(V), respectively. The oxidation process was dependent on the bacterial growth rate. Reduction of As accumulation was observed in the rice seedlings inoculated with strain SMS06 or SMS11. The total As concentrations in the rice seedlings inoculated with the strains were decreased up to 42.0% compared to that without bacteria inoculation. The results indicated these microorganisms as potential candidates for remediation of As(III) contamination in the environment.

Investigation of arsenic-resistant, arsenite-oxidizing bacteria for plant growth promoting traits isolated from arsenic contaminated soils.

The problem of arsenic (As) pollution being severe warrants opting for low-cost microbial remediation strategies. The present study of identifying suitable bacterial strains led to the isolation of eleven As-tolerant strains from the As-contaminated rhizosphere soils of West Bengal, India. They were found to oxidize/reduce 55-31.6% of 5mM As(III) and 73-37.6% of 5mM As(V) within 12h. The four isolates (BcAl-1, JN 73, LAR-2, and AR-30) had a high level of As(III) oxidase activity along with a higher level of As(V) and As(III) resistance. The agar diffusion assay of the isolates further confirmed their ability to endure As stress. The presence of aoxB gene was observed in these four As(III) oxidizing isolates. Evaluation of plant growth-promoting characteristics revealed that BcAl-1 (Burkholderia cepacia), JN 73 (Burkholderia metallica), AR-30 (Burkholderia cenocepacia), and LAR-2 (Burkholderia sp.) had significant plant growth-promoting characteristics (PGP), including the ability to solubilize phosphate, siderophore production, indole acetic acid-like molecules production, ACC deaminase production, and nodule formation under As stressed condition. BcAl-1 and JN 73 emerged as the most promising traits in As removal as well as plant growth promotion.

Arsenic speciation, the abundance of arsenite-oxidizing bacteria and microbial community structures in groundwater, surface water, and soil from a gold mine

The arsenic speciation, the abundance of arsenite-oxidizing bacteria, and microbial community structures in the groundwater, surface water, and soil from a gold mining area were explored using the PHREEQC model, cloning-ddPCR of the aioA gene, and high-throughput sequencing of the 16S rRNA gene, respectively. The analysis of the aioA gene showed that arsenite-oxidizing bacteria retrieved from groundwater, surface water, and soil were associated with Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria. In groundwaters from the mining area, there were relatively high ratios of aioA/total 16S rRNA gene copies and the dominance of As5+, which suggested the presence and activity of arsenite-oxidizing bacteria. Metagenomic analysis revealed that the majority of the soil and surface water microbiomes were Proteobacteria, Actinobacteria, Bacteroidetes, and Chloroflexi, whereas the groundwater microbiomes were dominated exclusively by Betaproteobacteria and Alphaproteobacteria. Geochemical factors influencing the microbial structure in the groundwater were As, residence time, and groundwater flowrate, while those showing a positive correlation to the microbial structure in the surface water were TOC, ORP, and DO. This study provides insights into the groundwater, surface water, and soil microbiomes from a gold mine and expands the current understanding of the diversity and abundance of arsenite-oxidizing bacteria, playing a vital role in global As cycling.

Soil microbial response to silicate fertilization reduces bioavailable arsenic in contaminated paddies

Rice grown in arsenic (As)-contaminated soils will have an elevated As concentration in the edible parts and lower yield due to the stressful growing environment. Silicon fertilizer is a soil amendment that can prevent As bioaccumulation and increase rice yield. This may be due to chemical interactions between silicon and As, but we hypothesized that silicon fertilization would improve soil microbial functions that control the bioavailable As concentration and improve the growing environment for rice in As-contaminated agroecosystems. We tested the hypothesis on two geographically different rice varieties (Indica and Japonica) grown on As spiked soils. Rice grown in pots amended with slag-based silicon fertilizer (silicate fertilizer) at a rate of 2 Mg ha−1 had a significantly lower As concentration in the grain, with 28% less As in the grain of Indica rice and 30% less As in the Japonica rice grain. The rice grain yield was 20–22% greater in these rice varieties. There are several reasons that As uptake declined in the rice grain, including: (i) a reduction in As bioavailability, likely because As was absorbed to amorphous and crystalline iron oxides in the soil and on root Fe-plaque, (ii) an increase in As resistant and arsenite-oxidizing bacteria that likely contribute to the natural attenuation of As in soil, and (iii) an increase in the pore-water silicon concentration that competitively inhibited As uptake by plants. Adding silicate fertilizer improved the general soil fertility by increasing the abundance of microbial functional communities involved in nutrient (carbon, nitrogen, and phosphorus) cycling, nitrogen and carbon fixation, methane consumption, and heavy metals/metalloid detoxification, while decreasing the number of microorganisms involved in methane and nitrous oxide production. We conclude that silicate fertilizer amendments stimulate soil microbial functions that are beneficial for rice growth while controlling As bioavailability, and thus are a potential solution to allow rice production in As-contaminated paddy soils.

Adaptation of Microbial Communities to Environmental Arsenic and Selection of Arsenite-Oxidizing Bacteria From Contaminated Groundwaters.

Arsenic mobilization in groundwater systems is driven by a variety of functionally diverse microorganisms and complex interconnections between different physicochemical factors. In order to unravel this great ecosystem complexity, groundwaters with varying background concentrations and speciation of arsenic were considered in the Po Plain (Northern Italy), one of the most populated areas in Europe affected by metalloid contamination. High-throughput Illumina 16S rRNA gene sequencing, CARD-FISH and enrichment of arsenic-transforming consortia showed that among the analyzed groundwaters, diverse microbial communities were present, both in terms of diversity and functionality. Oxidized inorganic arsenic [arsenite, As(III)] was the main driver that shaped each community. Several uncharacterized members of the genus Pseudomonas, putatively involved in metalloid transformation, were revealed in situ in the most contaminated samples. With a cultivation approach, arsenic metabolisms potentially active at the site were evidenced. In chemolithoautotrophic conditions, As(III) oxidation rate linearly correlated to As(III) concentration measured at the parental sites, suggesting that local As(III) concentration was a relevant factor that selected for As(III)-oxidizing bacterial populations. In view of the exploitation of these As(III)-oxidizing consortia in biotechnology-based arsenic bioremediation actions, these results suggest that contaminated aquifers in Northern Italy host unexplored microbial populations that provide essential ecosystem services.

Effects of Arsenic and Iron on the Community and Abundance of Arsenite-Oxidizing Bacteria in an Arsenic-Affected Groundwater Aquifer

Arsenic (As) contamination of groundwater aquifers is a global environmental problem, especially in South and Southeast Asian regions, and poses a risk to human health. Arsenite-oxidizing bacteria that transform As(III) to less toxic As(V) can be potentially used as a groundwater As remediation strategy. This study aimed to examine the community and abundance of arsenite-oxidizing bacteria in groundwater with various As concentrations from Rayong Province, Thailand using PCR-cloning-sequencing and quantitative PCR (qPCR) of catalytic subunit of arsenite oxidase gene (aioA). Key factors influencing their community and abundance were also identified. The results demonstrated that arsenite-oxidizing bacteria retrieved from groundwater were phylogenetically related to Betaproteobacteria and Alphaproteobacteria. The aioA gene abundances ranged from 8.6 × 101 to 1.1 × 104 copies per ng of genomic DNA, accounting for 0.16-1.37% of the total 16S rRNA bacterial gene copies. Although the abundance of arsenite-oxidizing bacteria in groundwater was low, groundwater with As(III) dominance likely promoted their abundance which possibly played an important role in chemolithoautotrophic oxidation of As(III) to As(V). Fe and As(III) were the major environmental factors influencing the community and abundance of arsenite-oxidizing bacteria. The knowledge gained from this study can be used to further contribute to the development of bioremediation strategies for As removal from groundwater resources.

Microbial community structure in aquifers associated with arsenic: analysis of 16S rRNA and arsenite oxidase genes.

The microbiomes of deep and shallow aquifers located in an agricultural area, impacted by an old tin mine, were explored to understand spatial variation in microbial community structures and identify environmental factors influencing microbial distribution patterns through the analysis of 16S rRNA and aioA genes. Although Proteobacteria, Cyanobacteria, Actinobacteria, Patescibacteria, Bacteroidetes, and Epsilonbacteraeota were widespread across the analyzed aquifers, the dominant taxa found in each aquifer were unique. The co-dominance of Burkholderiaceae and Gallionellaceae potentially controlled arsenic immobilization in the aquifers. Analysis of the aioA gene suggested that arsenite-oxidizing bacteria phylogenetically associated with Alpha-, Beta-, and Gamma proteobacteria were present at low abundance (0.85 to 37.13%) and were more prevalent in shallow aquifers and surface water. The concentrations of dissolved oxygen and total phosphorus significantly governed the microbiomes analyzed in this study, while the combination of NO3--N concentration and oxidation-reduction potential significantly influenced the diversity and abundance of arsenite-oxidizing bacteria in the aquifers. The knowledge of microbial community structures and functions in relation to deep and shallow aquifers is required for further development of sustainable aquifer management.

A novel biofilm bioreactor derived from a consortium of acidophilic arsenite-oxidizing bacteria for the cleaning up of arsenite from acid mine drainage.

Arsenite (As(III)) was considered to be of great concern in acid mine drainage (AMD). A promising approach for cleaning up of arsenite from AMD is microbial oxidation of As(III) followed by adsorptions. However, there is virtually no research about the acidophilic bioreactor for As(III) oxidation so far. In this study, we formed a new biofilm bioreactor with a consortium of acidophilic As(III) oxidation bacteria. It is totally chemoautotrophic, with no need to add any carbon or other materials during the operations. It works well under pH 3.0-4.0, capable of oxidizing 1.0-20.0 mg/L As(III) in 3.0-4.5 h, respectively. A continuous operation of the bioreactor suggests that it is very stable and sustainable. Functional gene detection indicated that the biofilms possessed a unique diversity of As(III) oxidase genes. Taken together, this acidophilic bioreactor has great potential for industrial applications in the cleaning up of As(III) from AMD solution.

Improved grain yield and lowered arsenic accumulation in rice plants by inoculation with arsenite-oxidizing Achromobacter xylosoxidans GD03

Arsenite is the predominant arsenic species in flooded paddy soil, and arsenite bioaccumulation in rice grains has been identified as a major problem in many Asian countries. Lowering arsenite level in rice plants and grain via accelerating arsenite oxidation is a potential strategy to help populations, who depended on rice consumption, to reduce the internal exposure level of arsenic. We herein isolated a strain, Achromobacter xylosoxidans GD03, with the high arsenite-oxidizing ability and plant growth-promoting traits. We observed that arsenite exposure could promote A. xylosoxidans GD03 to excrete indole-3-acetic acid and thus promoted rice growth. The pot culture experiments of Indica rice cultivar Guang You Ming 118 (GYM118) demonstrated that A. xylosoxidans GD03 inoculation of paddy soil (4.5–180 × 108 CFU GD03/kg soil) significantly accelerated arsenite oxidation in flooded soil. The daily arsenic oxidation rate with GD03 inoculation was 1.5–3.3 times as that without strain GD03 inoculation within the whole growth period of Indica GYM118 in the presence of the native microflora. It thus led to a 34–69%, 43–74%, 24–76% and 35–57% decrease in arsenite concentration of the stems, leaves, bran and grain of Indica GYM118 respectively and a 59–96% increase in rice grain yield. The paddy soil inoculated with 40.0 mL/kg of A. xylosoxidans GD03 resulted in a lowest As(III) concentrations in all rice organs of Indica GYM118, which equivalent to only 24–50% of the As(III) concentrations in the group without GD03 inoculation. The results highlight that a highly arsenite-oxidizing bacterium could accelerate arsenite oxidation of paddy soil when facing competition with the native microflora, thus decrease arsenic toxicity and bioavailable soil arsenic.

Genomic and physiological characterization of an antimony and arsenite-oxidizing bacterium Roseomonas rhizosphaerae

Antimony (Sb) and arsenic (As) are two toxic metalloids, which are listed as priority environmental pollutants by the European Union and the U.S. Environmental Protection Agency (EPA). Antimony taken up by plants enters the food chain and poses a threat to human health. Microbial oxidation of antimonite (Sb(III)) and arsenite (As(III)) to the less toxic antimonate (Sb(V)) and arsenate (As(V)), has great potential for the immobilization of Sb and As in the environment. A heterotrophic aerobic bacterium, Roseomonas rhizosphaerae YW11, oxidized both Sb(III) and As(III) in the modified R2A medium. In the same medium, strain YW11 preferred to oxidize Sb(III), whereas the As(III) oxidation rate was only 50%. Genomic analysis of YW11 confirmed the presence of several As-resistance gene islands. The aioAB genes encoding As(III) oxidase were also induced by Sb(III). The role of aioA in Sb(III) oxidation and resistance was confirmed by disrupting this gene in strain YW11, resulting in the loss of Sb(III) oxidation abilities. This study documents an enzymatic basis for microbial Sb(III) oxidation in strain YW11, which is a novel bacterial strain showing simultaneous oxidation of Sb(III) and As(III), and may be a potential candidate for bioremediation of heavy metal-contaminated environments.

Enhancing the plants growth and arsenic uptake from soil using arsenite-oxidizing bacteria

Plants, that naturally inhabit arsenic-contaminated areas may be used for effective arsenic-uptake from soil. The efficiency of this process may be increased by the reducing arsenic phytotoxicity and stimulating the activity of indigenous soil microbiota. As we showed, it can be achieved by the bioaugmenting of soil with arsenite-oxidizing bacteria (AOB). This study aimed to investigate the influence of soil bioaugmentation with AOB on the structure, quantity, and activity of the indigenous soil microbiota as well as to estimate the effect of such changes on the morphology, growth rate, and arsenic-uptake efficiency of plants. Plants-microbes interactions were investigated using the effective arsenites oxidizer Ensifer sp. M14 and the native plant alfalfa. The experiments were performed both in potted garden soil enriched with arsenic and in highly arsenic polluted, natural soil. The presence of M14 strain in soil contributed to the increase both in plants growth intensity and arsenic-uptake efficiency with regard to the soil without M14. After 40 days of plants culture, their average biomass increased by about 60% compared to non-bioaugmented soil, while the arsenic accumulation increased more than two times. The soil bioaugmentation contributed also to the increase in the quantity and activity of soil microorganisms without disturbing the natural microbial community structure. In the bioaugmented soil, the noticable increase in the quantity of heterotrophic, denitrifying, nitrifying and cellulolytic bacteria as well as in the activity of dehydrogenases and cellulases were observed. Soil bioaugmentation with M14 enables the application of native and commonly occurring plant species for enhancing the treatment of arsenic-contaminated soil. This in situ strategy may constitute a valuable alternative both to the chemical and physical methods of arsenic removal from soil and to the biological ways based on the arsenic hyperaccumulating plants and/or the arsenic mobilizing bacteria.

A simple spectrophotometric method for rapid quantitative screening of arsenic bio-transforming bacteria

Abstract A simple spectrophotometric technique for inorganic arsenic speciation of known total arsenic has been developed for application in quantitative screening of arsenite-oxidizing and arsenate-reducing bacteria from the wide range of environmental isolates. The technique is based on the spectrophotometric absorbance measurement of the color intensity in the arsenic and potassium permanganate mixture. Since arsenate does not react with potassium permanganate, the intensity of color decreases proportionately with the increasing concentration of arsenite in the total arsenic. The speciation method can be used over a pH range of 4–9. The validity of the results obtained from this spectrophotometric method was confirmed with the Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry (LC-ICP-MS). This method has great potential for the screening of arsenite-oxidizing bacteria.