Complex Arrangement Research Articles

An ultrastructure analysis of the developing human anterior cruciate ligament tibial enthesis.

This study aimed to investigate the ultrastructural anatomy of the developing ACL tibial enthesis. We hypothesized that enthesis architecture would progressively mature and remodel, eventually resembling that of the adult by the early postnatal stage. Five fresh-frozen human pediatric cadaveric knees aged 1-36 months underwent anatomical dissection to harvest the ACL insertion and underlying tibial chondroepiphysis. The samples were prepared for scanning electron microscopy (SEM) to examine the ultrastructural anatomy of the enthesis and underwent histological staining for circular polarized light (CPL) and light microscopy imaging. SEM analysis of the 1- and 8-month-old samples revealed a shallow interdigitation between the dense fibrous (ligamentous) tissue and unmineralized chondrogenic tissues, with a minimal transition zone. By 11-month, a more complex transition zone was present. By age 19- and 36-month-old, a progressively more complex and defined fibrocartilage zone was observed. CPL analysis revealed distinct collagen fiber continuity, alignment, and organization changes over time. By 19 and 36 months, the samples exhibited complex fiber arrangements and a progression toward uniform fiber orientation. Similarly, histological analysis demonstrated progressive remodeling of the enthesis with increasing age. Our results suggest that the ACL enthesis of the developing knee begins to mimic that of an adult as early as 19 months of age, as a more complex transition between ligamentous and chondro-epiphyseal tissue can be appreciated. We hypothesize that the observed changes are likely due to mechanical loading of the enthesis with the onset of weightbearing. Future investigations of ACL reconstruction and repair will benefit from improved understanding of the chondro-epiphyseal/ACL regions.

Free Reflection Multiarrangements and Quasi-Invariants

Abstract To a complex reflection arrangement with an invariant multiplicity function one can relate the space of logarithmic vector fields and the space of quasi-invariants, which are both modules over invariant polynomials. We establish a close relation between these modules. Berest–Chalykh freeness results for the module of quasi-invariants lead to new free complex reflection multiarrangements. Saito’s primitive derivative gives a linear map between certain spaces of quasi-invariants. We also establish a close relation between non-homogeneous quasi-invariants for root systems and logarithmic vector fields for the extended Catalan arrangements. As an application, we prove the freeness of Catalan arrangements corresponding to the non-reduced root system $BC_{N}$.

Enhancement of Single-Molecule Magnet Properties by Manipulating Intramolecular Dipolar Interactions.

A new single-molecule magnet (SMM) complex [K(18-crown-6)][(COT)Er(µ-Cl)3Er(COT)] (Er2Cl3, COT=cyclooctatetraenide dianion) is obtained by the reaction of [(COT)Er(µ-Cl)(THF)]2 (Er2Cl2, THF=tetrahydrofuran) with an equivalent of KCl in the presence of 18-crown-6. The two COT-Er units in the newly formed complex are triply bridged by µ-Cl ligands, leading to the "head-to-tail" alignment of the magnetic easy axes distinctly different from the "staggered" arrangement in the precursor complex. This structural transformation has led to significantly enhanced intramolecular dipolar interactions and a reduced transverse component of the crystal fields, increasing the energy barrier from 150(8) K for Er2Cl2 to 264(4) K for Er2Cl3 and extending its magnetic relaxation time at 2 K by 2500 times with respect to Er2Cl2. More importantly, the blocking temperature increased from lower than 2 K for Er2Cl2 to 8 K for Er2Cl3, and the magnetic hysteresis loops at 2 K changed from butterfly-shaped for Er2Cl2 to open hysteresis loop with coercive force of 7 kOe for Er2Cl3. These results suggest that the properties of SMMs can be effectively tuned and improved by rationally arranging magnetic spins via molecular engineering.

The Potential Application of Mung Bean (Vigna radiata L.) Protein in Plant‐Based Food Analogs: A Review

ABSTRACTThe increasing demand for plant‐based food products by consumers along with the growing global population requires the discovery of novel and sustainable protein sources to address environmental challenges and meet nutritional requirements. Among various plant proteins, mung bean protein (MBP) exhibits several unique characteristics that make it a valuable ingredient in the field of plant‐based food analogs. This literature review aims to express the unique structure and composition of MBP, in addition to its physicochemical, functional, and nutritional properties. Furthermore, its potential applications in novel meat, dairy, and egg analogs are highlighted to meet the growing demand for sustainable, nutritious, and delicious plant‐based food alternatives. Structurally, MBP consists of a complex arrangement of amino acids, forming a globular protein with distinct functional properties. Its composition is also rich in essential amino acids, particularly leucine and lysine, making it a promising protein source for plant‐based diets. From a techno‐functional perspective, MBP exhibits remarkable gelling, emulsifying, foaming, and binding properties, which are necessary for the development of stable emulsions, airy foams, firm gels, and cohesive textures in various plant‐based food formulations. Moreover, MBP and its derivatives can possess notable bio‐functional properties, including antioxidant activity and anti‐inflammatory benefits, as well as cholesterol‐lowering, anti‐obesity, antidiabetic, antihypertensive, and anticancer effects. Consequently, the production of food analogs based on MBP not only improves the techno‐functional attributes of the final products but also can promote consumer health and well‐being.

A Composite Fault Model for the 2024 MW 7.4 Hualien Earthquake Sequence in Eastern Taiwan Inferred From GNSS and InSAR Data

AbstractOn 2 April 2024, an MW 7.4 earthquake struck the northern Longitudinal Valley in eastern Taiwan, about 18 km SSW of Hualien, causing damage and casualties. In this study, we investigated a comprehensive geodetic data set, employing Global Navigation Satellite Systems (GNSS) and Interferometric Synthetic Aperture Radar (InSAR) measurements to assess the rupture geometry associated with earthquake sequence. Although geodetic data can be satisfactorily reproduced by simple single‐fault models (i.e., a high‐angle E‐dipping plane related to the Longitudinal Valley Fault (LVF), or a gentle W‐dipping surface associated with the Central Range Fault, CRF), a composite model involving the rupture of different fault segments (a major CRF‐related W‐dipping fault, a deep segment of the E‐dipping LVF, and the Milun Fault) is able to explain the observations, the distribution of seismicity, and the complex structural arrangement of the northernmost sector of the Longitudinal Valley.

Green space-building integration for Urban Heat Island mitigation: Insights from Beijing's fifth ring road district

In this research, we delve into the complex arrangement of urban landscapes, where green spaces and buildings are not merely co-existing but are interwoven into a cohesive fabric that shapes the thermal environment. Our approach transcends the conventional methods of analysis, which typically isolate the roles of greenery or built environments. Instead, we adopt a synergistic perspective that recognizes the collective influence of these landscape constituents on the urban thermal pattern. Key insights are: (1) A linear decrease in average land surface temperature with increasing green space coverage is observed. However, substantial temperature variations (up to 8 °C) within the same coverage interval highlight the significant impact of built-up pattern on thermal conditions; (2) High Building Height and Floor Area Ratio, and low Building Coverage Ratio and Sky View Factor, are linked to cooler temperatures in areas with up to 50 % green space; (3) The study suggests that low-temperature areas can inform the adjustment of built-up patterns in high-temperature areas, offering a strategy for thermal environment optimization within specific green space coverage intervals. This research contributes insights into the integrated planning of green spaces and buildings, with implications for urban development and renewal initiatives aiming to enhance the urban thermal environment.

Evaluation of the roughness of lattice structures of AISI 316 stainless steel produced by laser powder bed fusion

The present work evaluates the surface roughness of lattice structures produced in AISI 316 stainless steel, with laser powder bed fusion (LPBF). To this end, five lattice types were evaluated, namely cubic (C), truncated octahedron (TO), truncated cubic (TC), rhombicuboctahedron (RCO) and rhombitruncated cuboctahedron (RTCO) to determine the average roughness Sa by profilometry. For comparison, the surface roughness of a bulk specimen was also measured. The average roughness was Sa=7.3 mm for the bulk specimen, for the C and TC arrangements it was around 11 mm, while for configurations RCO, RTCO and TO it was in the range 18-22 mm, meaning that surface roughness increases for more complex structures. Values of Sa around 10-25 mm are reported in the literature for parts fabricated by LPBF for medical implants. If complex lattice arrangements are required, a study of the printing parameters is relevant, to achieve scaffolds with improved biomechanical performance.

A Deep-learning-based Auto Encoder-Decoder Model for Denoising Electrocardiogram Signals

Learning-based denoising techniques have become superior to the traditional assumption-based denoising methods in this modern era. Also, with the advancement of wearable technologies and remote electrocardiogram (ECG) monitoring systems, the requirement for optimal storage has increased due to the limited availability of hardware resources. Therefore, denoising and compression both are essential at the preprocessing stage of the ECG signal. Deep learning-based denoising auto encoder-decoder (DAED) models guarantee cutting-edge performance for these tasks. This article presents a lightweight, adaptive, hybrid Convolutional Neural Network-Gated Recurrent Unit (CNN-GRU) based DAED model that achieves a signal compression ratio of 64 with high signal-to-noise ratio improvement for the elimination of ECG noises. The novelty of this work lies in the customization of the CNN layers and utilization of the advantages of the GRU layer in a proper channel for compression and denoising ECG signals. The comparative study with other complex deep learning-based DAED arrangements and state-of-the-art denoising techniques shows the proposed model has simplicity in construction and an improved signal-to-noise ratio with minimum mean square error.

What Is the Crystallographic Resolution of Structural Models of Proteins Generated with AlphaFold2?

Recent advancements in AI-driven computational modeling, especially AlphaFold2, have revolutionized the prediction of biological macromolecule structures. AlphaFold2 enabled accurate predictions of structural domains and complex arrangements. However, computational models lack a clear metric for accuracy. This study explores whether computational models can match the crystallographic resolution of crystal structures. By comparing distances between atoms in models and crystal structures using t tests, it was found that AlphaFold2 models are comparable to high-resolution crystal structures (1.1 to 1.5 Å). While these models exhibit exceptional quality, their accuracy is lower than the crystal structure with resolutions better than 1 Å.

Diversity and Complexity of CTXΦ and Pre-CTXΦ Families in Vibrio cholerae from Seventh Pandemic.

CTXΦ is a lysogenic filamentous phage that carries the genes encoding cholera toxin (ctxAB), the main virulence factor of Vibrio cholerae. The toxigenic conversion of environmental V. cholerae strains through CTXΦ lysogenic infection is crucial for the emergence of new pathogenic clones. A special allelic form of CTXΦ, called pre-CTXΦ, is a precursor of CTXΦ and without ctxAB. Different members of the pre-CTXΦ and CTXΦ families are distinguished by the sequence of the transcriptional repressor-coding gene rstR. Multiple rstR alleles can coexist within a single strain, demonstrating the diverse structure and complex genomic integration patterns of CTXΦ/pre-CTXΦ prophage on the chromosome. Exploration of the diversity and co-integration patterns of CTXΦ/pre-CTXΦ prophages in V. cholerae can help to understand the evolution of this phage family. In this study, 21 V. cholerae strains, which were shown to carry the CTXΦ/pre-CTXΦ prophages as opposed to typical CTXETΦ-RS1 structure, were selected from approximately 1000 strains with diverse genomes. We identified two CTXΦ members and six pre-CTXΦ members with distinct rstR alleles, revealing complex chromosomal DNA integration patterns and arrangements of different prophages in these strains. Promoter activity assays showed that the transcriptional repressor RstR protected against CTXΦ superinfection by preventing the replication and integration of CTXΦ/pre-CTXΦ phages containing the same rstR allele, supporting the co-integration of the diverse CTXΦ/pre-CTXΦ members observed. The numbers and types of prophages and their co-integration arrangements in serogroup O139 strains were more complex than those in serogroup O1 strains. Also, these CTXΦ/pre-CTXΦ members were shown to present the bloom period of the CTXΦ/pre-CTXΦ family during wave 2 of the seventh cholera pandemic. Together, these analyses deepen our comprehension of the genetic variation of CTXΦ and pre-CTXΦ and provide insights into the evolution of the CTXΦ/pre-CTXΦ family in the seventh cholera pandemic.

Regulatory challenges in modernizing toll road transaction systems in Indonesia

The Government of Indonesia has modernized the toll road transaction system by implementing the multi-lane free-flow (MLFF) project, set to operate commercially by the end of 2024. This project leverages Global Navigation Satellite System (GNSS) technology to identify vehicles using toll roads and establish a transaction mechanism that allows the MLFF Project Company to charge road users according to distance, vehicle category, and tariff levels. The project has result in a complex business arrangement between the Indonesia National Toll Road Authority (INTRA), Toll Road Companies (TRCs), and the MLFF Project Company. The aim of this paper is to review the regulatory and institutional framework of the MLFF project and analyze its challenges. The methodology employed is a qualitative framework for legal research, utilizing international literature reviews and current regulatory frameworks. The study assesses the proposed transaction architecture of the project and identifies commercial, political, and other risks associated with its implementation. Based on the analysis, the research identifies opportunities for regulatory improvements and better contracting arrangements. This research provides valuable insights into the regulatory landscape and offers policy recommendations for the Government to mitigate the identified risks. This contribution is significant to the academic field as it enhances understanding regulatory and institutional challenges in implementing advanced toll road systems.

Determination of Hydrogen Bonds in GROMACS: A New Implementation to Overcome Memory Limitation.

This work introduces a new faster implementation of the hydrogen bond network (complex arrangement of hydrogen bonds between or within molecules) search algorithm in biomacromolecules and their environment. Existing implementation of such an algorithm in GROMACS [Abraham et al. GROMACS 2024.2 Manual. 2024.] has limitations in the analysis of large structures and trajectories. The new implementation, in the form of a native GROMACS trajectory analysis module, allows for quick analysis of molecular dynamics trajectories without restrictions, thus overcoming the limitations of the original algorithm. The application of the developed method enabled the acquisition and analysis of hydrogen bond networks in the studied defensin-like protein Pentadiplandra brazzeana, as well as the study of hydrogen bond occupancies between the protein's residues and water molecules. The data obtained using the new implementation coincided with the experimental data.

One dimensional water chain as a zipper in a new polymorph of trans [Cu(vanilin)2(H2O)2].2(H2O) crystal–A case of water induced polymorphism

A new polymorph [Cu(II)(vanillin)2(H2O)2].2(H2O) has been synthesized and characterized by single crystal X-ray structural studies. Two other crystals having same formula and coordination unit but differing supramolecular arrangement due to slightly different role played by water molecules have already been reported in the literature [B. Kozlevˇcar et al. Z. Naturforsch., B: Chem. Sci. 2005, 60, 1273][Kozlevčar et al. Acta Chim. Slov. 2005, 52, 40–43]. The current compound is another polymorph in this system which has arisen again due to a different supramolecular arrangement which is water induced. Hirshfeld surface and energy framework analysis have been employed to compare these three polymorphs by assessing the nature of intermolecular interactions. In the current compound strong OH···O hydrogen bonding interactions between coordinated water molecules and –OH group attached to vanillin ligand leads to parallel alignment of successive coordination units leading to a supramolecular ribbon topology that runs along the crystallographic a-axis. A zigzag chain of single file water chain is positioned in between successive ribbons which acts as a zipper giving rise to a 2D supramolecular arrangement of the molecular complexes. In two other reported polymorphs instead of a single file water chain a cyclic water motif has been observed. The electronic structure of the complex explored using DFT methodology. This set of three polymorphs is an interesting example of water induced polymorphism.

Features that characterize monoclonal light chain ("myeloma") cast nephropathy with immunofluorescence challenges and emphasis on electron microscopy.

Renal disease is a common cause of morbidity and mortality in patients with plasma cell dyscrasias. The serum-free light chain assay is used in patients, mostly older, with unexplained acute kidney injury to screen for potential myeloma cast nephropathy. This study consists of a systematic review of diagnostic features in myeloma cast nephropathy. The morphological features of tubular casts in patients with multiple myeloma have not been systematically analyzed. This study focuses on the morphology of these casts, emphasizing ultrastructural features, in a series of 23 patients with light chain ("myeloma") cast nephropathy and compared them with casts in 10 patients with various diseases. The immunofluorescence data were correlated with morphological findings to provide diagnostic assessments and practice guidelines. The ultrastructural features identified as diagnostic of casts associated with myeloma included: amyloid and crystals in the casts, multiple well-defined fracture planes forming a complex jigsaw puzzle arrangement of cast contents, indicative of the fragility of the immunoglobulin light chains involved, and reactive tubular cells lining the tubules with the casts. These features were seen in 95.2% of MCN cases and none of the casts in other renal conditions. Myeloma casts exhibited light chain monoclonality in a significant percentage of the MCN cases and often no staining for IgA or IgM. In contrast, the majority of non-myeloma casts stained for both kappa and lambda light chains, lgA, and lgM, and showed ultrastructurally a rather uniform finely to coarsely granular electron density occasionally admixed with cellular debris.

Programmable Analog Circuits with Neuromorphic Nanostructured Platinum Films

AbstractSelf‐assembled nanoparticle or nanowire networks have recently come under the spotlight as systems able to emulate brain‐like data processing performances by exploiting the memristive character and the wiring of the junctions connecting the nanostructured network building blocks. Recently it is demonstrated that nanostructured Au films, fabricated by the assembling of gold clusters produced in the gas phase, have non‐linear and non‐local electric conduction properties caused by the extremely high density of grain boundaries and the resulting complex arrangement of nanojunctions. These observations suggest that nanostructured metallic films can be explored and exploited as a novel class of neuromorphic systems for unconventional electronic devices. Here it is reported that nanostructured cluster‐assembled Pt films show resistive switching activity, negative differential resistance, and reversible memory effects. These properties allow the use of nanostructured Pt films in programmable analog circuits, such as gain amplifiers and relaxation oscillators with fast response.

Transparency and public accountability: Does the Nigerian extractive industries transparency initiative deliver?

The extractive industries play a pivotal role in Nigeria's economy, yet it has been riddled with allegations of corrupt practices involving revenue transfers from operations to the government. To increase transparency and ensure public engagement, the Nigerian Extractive Industries Transparency Initiative (NEITI) introduced the National Stakeholder Working Group (NSWG) as a mechanism to ensure public accountability and civil society organisations (CSOs) as the public interlocuter. To navigate this complex arrangement and critically evaluate its effectiveness, we adopt Boven's (2007) understanding of public accountability. We conclude that while NSWG provides oversight, the NEITI reporting process provides transparency, and the CSOs enable public accountability, these objectives are constrained by a challenging social and regulatory environment.

Study of the influence of macro-structure and micro-structure on the mechanical properties of stag beetle upper jaw

Macrostructural control of stress distribution and microstructural influence on crack propagation is one of the strategies for obtaining high mechanical properties in stag beetle upper jaws. The maximum bending fracture force of the stag beetle upper jaw is approximately 154, 000 times the weight of the upper jaw. Here, we explore the macro and micro-structural characteristics of two stag beetle upper jaws and reveal the resulting differences in mechanical properties and enhancement mechanisms. At the macroscopic level, the elliptic and triangular cross-sections of the upper jaw of the two species of stag beetles have significant effects on the formation of cracks. The crack generated by the upper jaws with a triangular section grows slowly and deflects easily. At the microscopic level, the upper jaw of the two species is a chitin cross-layered structure, but the difference between the two adjacent fiber layers at 45° and 50° leads to different deflection paths of the cracks on the exoskeleton. The mechanical properties of the upper jaw of the two species of stag beetle were significantly different due to the interaction of macro-structure and micro-structure. In addition, a series of bionic samples with different cross-section geometries and different fiber cross angles were designed, and mechanical tests were carried out according to the macro-structure and micro-structure characteristics of the stag beetle upper jaw. The effects of cross-section geometry and fiber cross angle on the mechanical properties of bionic samples are compared and analyzed. This study provides new ideas for designing and optimizing highly loaded components in engineering. Statement of significanceThe upper jaw of the stag beetle is composed of a complex arrangement of chitin and protein fibers, providing both rigidity and flexibility. This structure is designed to withstand various mechanical stresses, including impacts and bending forces, encountered during its burrowing activities and interactions with its environment. The study of the upper jaw of the stag beetle can provide an efficient structural design for engineering components that are subjected to high loads. Understanding the relationship between structure and mechanical properties in the stag beetle upper jaw holds significant implications for biomimetic design and engineering.

Hurst Castle: collapse, stabilisation and future

Hurst Castle, a Henrician artillery fort with two Victorian wing batteries and scheduled monument, sits on the end of a shingle spit at the entrance to the Solent. Changes in long shore drift, increasing sea levels and more frequent storms led to the collapse of a section of the nineteenth century east wing battery in February 2021. The vaulted masonry structure of the west wing battery also sustained significant structural damage. English Heritage have since embarked on an ambitious project to attempt to stabilise the castle. A new permanent revetment to protect the most vulnerable area in front of the east wing and geotechnical investigations to allow the severely damaged west wing to be assessed and repaired have already been completed. The health and safety challenges and complex access, either by way of boat or along the mile-long shingle spit, have required the adoption of innovative design solutions and complex monitoring arrangements. Funds permitting, the next phase of works will include permanent structural repairs to the west wing along with further coastal protection work to ensure the longevity of the castle. However, before this can begin, complex questions around the future of the spit and the implications of climate-related loss in the long term must be answered.

Calusa Fisheries and Estuarine Socio-Ecologies in Southwestern Florida: An Examination of Large-Bodied Fish

ABSTRACT Mound Key (8LL2), a 51 ha anthropogenic island in Estero Bay, Florida, is comprised of a complex arrangement of midden-mounds of various sizes, canals, watercourts, causeways, and other features. Occupied from approximately a.d. 450 through European contact, Mound Key served as the primary center and political capital of an extensive Calusa socioeconomic network whose economy was rooted in fishing, gathering, and hunting. Drawing on zooarchaeological analyses of ichthyological refuse dating to between a.d. 1000 and 1350, I argue that the Calusa actively managed and invested in a core fishery that ultimately contributed to a complex, robust socio-ecological systems (SES). I examine the nature, role, and signatures of these fisheries, as their management underwrote the success of Calusa social, political, and economic systems.

Structural Basis of Cucurbituril-Containing Self-Assembled Supramolecular Chiral Materials.

Macrocycle-based host-guest complexation offers an intriguing protocol in producing chiroptical materials, while the bulky size and dynamic exchange between hosts and guests hinders the ordered aggregation to afford the long-range chiral arrangement. It remains great challenges in assembling cucurbit[n]urils (CB[n]s) included complexes to induce supramolecular chirality ascribed to the excellent water solubility and flexible packing. Herein, we unveiled the structural basis on the formation of chiroptical coassemblies from CB[n] (n=6, 7) complexes. Perylene diimides (PDIs) with cationic chiral pendants formed complexes in the aqueous media, which selectively showed chiroptical properties. Chlorination at the bay position, increasing alkyl length of cationic chiral pendants or reducing the number of polyaromatic rings would hinder the chiral aggregation. In a comprehensive manner, CB[6] favors ordered aggregation into one-dimensional fibrous nanoarchitectures that greatly facilitates the supramolecular chirality. In contrast, CB[7] with larger cavity and water solubility shrinks the ordered arrangement of complexes, reducing the formation possibility of supramolecular chiral nanoarchitectures. This work suggests the great potential of CB[6] in the preparation and manipulation of supramolecular chiral assemblies, shedding light on the macrocycle-based functional chiroptical materials.