A palynological and lithological continuous record from La Mancha Plain (South-central Iberian Peninsula, Spain) is presented. The obtained results have permitted the reconstruction of the climatic evolution in the area since the Late-glacial/Holocene transition. The end of the Late-glacial was characterized by a cold and arid climate and it concluded at about 9890±180 yr BP. In the Early Holocene a slight climatic amelioration began, being interrupted by a more arid phase around 8500 yr BP. The Holocene Climatic Optimum started from ca. 8000 yr BP and was characterized by higher temperatures and more humidity. During the Mid-Holocene–Late Holocene times a stage of marked aridity occurred towards 5000 yr BP that preceded the development of a dry mediterranean climate. Under this new climate a short-time arid phase took place around 2500 yr BP after which the mediterranean conditions became accentuated, with an increasing dry climate. This climatic evolution has affected the characteristics of the marshlands of Las Tablas de Daimiel National Park, originating changes in the sedimentary environment. This environment was mainly fluvial until the end of the Holocene Climatic Optimum and became lacustrine–palustrine from the beginning of the more arid conditions.