This Brief Report presents an example of assessment validation using an argument-based approach. The instrument we developed is a Brief Assessment of Students’ Mature Number Sense, which measures a central goal in mathematics education. We chose to develop this assessment to provide an efficient way to measure the effect of instructional practices designed to improve students’ number sense. Using an argument-based framework, we first identify our proposed interpretations and uses of student scores. We then outline our argument with three claims that provide evidence connecting students’ responses on the assessment with its intended uses. Finally, we highlight why using argument-based validation benefits measure developers as well as the broader mathematics education community.