This work explores the influence of sample temperature, ambient gas pressure, laser energy, and detector gate delay (DGD) on the LIBS emissions of extracted animal fat. The emissions from laser-induced plasma of extracted animal fat were acquired with varying sample temperatures, ambient argon pressure, laser energy, and detector gate delay. A thermoelectric system fitted with a temperature-controlling sensor was used to control the sample temperature. For maximum optical emission intensity, the detector gate delay (DGD) shifted toward a lower value with the increase in sample temperature for all ambient argon pressures. The nine-fold signal enhancement, ten times improvement in signal-to-noise ratio (SNR), and significantly reduced self-absorption were observed in all detected emission lines recorded at 200 mJ laser energy –2 ºC sample temperature, 375 Torr ambient argon pressure, and 2 µs DGD. The repeatability of emission signals was evaluated by calculating the relative standard deviation (RSD) of the emission line (Zn I (636.23 nm)) at different sample temperatures, which improved from 35 % to 6 % when the sample changed from liquid to solid (frozen). During the semi-liquid form of fat, the decreasing ambient gas pressure did not significantly affect signal repeatability. In addition to diagnosing the fat plasma, the plasma temperature and electron number density have been evaluated as a function of sample temperature and ambient argon pressure.
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