Vasotocin Research Articles

Functional interactions between vasotocin and prostaglandins during final oocyte maturation and ovulation in the catfish Heteropneustes fossilis

Functional interactions between vasotocin (VT) and prostaglandins (PGs) in the regulation of final oocyte maturation (FOM) and ovulation were investigated in the catfish Heteropneustes fossilis. Incubation of post-vitellogenic follicles with VT resulted in significant increases of both PGF2α and PGE2 at 8 and 16h intervals. The rise was higher at 16h except in the 1000nM VT group, in which the PG levels decreased compared to the 100nM group (biphasic effect). VT was more effective to increase the PG levels in comparison to hCG or IT. The co-incubation of the follicles with both hCG (20IU/ml) and VT (100nM) increased significantly PGF2α level at 8h, higher than that elicited by each when incubated alone. Pre-incubation of the follicles with V1 receptor antagonist, alone or in co-incubation with VT, significantly inhibited the VT-stimulated PGF2α and PGE2 levels. Under similar conditions, V2 receptor antagonist did not affect the PGE2 levels. Both VT (100nM) and PGs stimulated FOM (germinal vesicle breakdown) and ovulation in a dose- and duration dependent manner, PGF2α was more effective. Incubation of postvitellogenic follicles with indomethacin (a non selective cyclooxygenase inhibitor) per se did not affect FOM and ovulation but significantly decreased VT and PG effects upon pre-incubation. The results suggest that the VT stimulation of PGs may be mediated mainly through the V1 receptor though the involvement of V2 receptor cannot be excluded. The article also discussed the positive interplay of gonadotropin, maturation-inducing steroid, VT and PG during FOM and ovulation.

Distribution of the Vasotocin Subtype Four Receptor (VT4R) in the Anterior Pituitary Gland of the Chicken, Gallus gallus, and its Possible Role in the Avian Stress Response

The neurohormone arginine vasotocin (AVT) in non mammalian vertebrates is homologous to arginine vasopressin (AVP) in mammals. Its actions are mediated via G protein-coupled receptors that belong to the vasotocin/mesotocin family. Because of the known regulatory effects of nonapeptide hormones on anterior pituitary functions, receptor subtypes in that family have been proposed to be located in anterior pituitary cells. Recently, an avian vasotocin receptor subtype designated VT4R has been cloned, which shares 69% sequence homology with a human vasopressin receptor, the V1aR. In the present study, a polyclonal antibody to the VT4R was developed and validated to confirm its specificity to the VT4R. The antibody was used to test the hypothesis that the VT4R is present in the avian anterior pituitary and is specifically associated with certain cell types, where its expression is modulated by acute stress. Western blotting of membrane protein extracts from pituitary tissue, the use of HeLa cells transfected with the VT4R and peptide competition assays all confirmed the specificity of the antibody to the VT4R. Dual-labelling immunofluorescence microscopy was utilised to identify pituitary cell types that contained immunoreactive VT4R. The receptor was found to be widely distributed throughout the cephalic lobe but not in the caudal lobe of the anterior pituitary. Immunoreactive VT4R was associated with corticotrophs. Approximately 89% of immunolabelled corticotrophs were shown to contain the VT4R. The immunoreactive VT4R was not found in gonadotrophs, somatotrophs or lactotrophs. To determine a possible functional role of the VT4R and previously characterised VT2R, gene expression levels in the anterior pituitary were determined after acute immobilisation stress by quantitative reverse transcriptase-polymerase chain reaction. The results showed a significant increase in plasma corticosterone levels (three- to four-fold), a significant reduction of VT4R mRNA and an increase of VT2R mRNA (P < 0.05) in acutely immobilised chicks compared to controls. The data suggest a role of the VT4R in the avian stress response.

Stocking density influences brain arginine vasotocin (AVT) and isotocin (IT) levels in males and females of three-spined stickleback (Gasterosteus aculeatus)

Arginine vasotocin (AVT) and isotocin (IT) are fish neurohormones produced in separate parvocellular and magnocellular preoptic neurons of Teleostei. Apart from well-established peripheral action as hormones they are important neurotransmitters in central nervous system in fish. In the present study, we examined an influence of stocking density on whole brain AVT and IT concentrations in males and females of three-spined stickleback (Gasterosteus aculeatus). In males, the highest AVT levels have been found at stocking densities of 10 and 30 individuals per 30-l tank. On the other hand, in females, AVT concentration was significantly higher in those kept alone. Brain IT concentrations significantly increased along with stocking density only in females and did not change in males. The sex-dependent responses indicate a different stimulation of AVT and IT neurons in males and females. Consequently, roles of the neurohormones in males and females exposed to stress of overcrowding must be different.

Social Interaction Influences Blood Cortisol Values and Brain Aromatase Genes in the Protandrous False Clown Anemonefish,Amphiprion ocellaris

Anemonefish, Amphiprion spp., are socially controlled, protandrous sex changers with a monogamous mating system. Under certain conditions, sexually immature anemonefish with ambisexual gonads differentiate directly into males or females. Formation and maintenance of social rank in a group are considered key requirements for the induction of sex change or differentiation. Generally, each animal living in a social group experiences a different level of social stress in accordance with its social rank, and we hypothesize that the stress situation of individual anemonefish influences its sex determination. Groups of three sexually immature anemonefish were placed into each of five experimental tanks and kept for 10 days to allow for social rank formation and behavioral observation. The fish were then euthanized, and blood and brain samples were collected from each fish. The social rank of each individual was distinguishable from day 1 of the experiment. Aggressive behaviors were most frequent and blood Cortisol values were higher in dominant individuals. The transcription of mRNA for stress-related genes, i.e., those encoding for glucocorticoid and arginine vasotocin receptors, was higher in the brains of dominant individuals than in other social ranks. Furthermore, we detected higher transcription levels of gonad and brain aromatase genes, which encode the enzyme that converts androgens into estrogens, in the brains of dominant individuals. These results suggest that social rank reflects the blood Cortisol value, which in turn leads to sex differentiation by manipulating transcription of genes, including aromatase genes, in the brain.

Social rank‐dependent expression of arginine vasotocin in distinct preoptic regions in male Oryzias latipes

This study examined arginine vasotocin (AVT) expression in the brains of dominant and subordinate male medaka Oryzias latipes after short- and long-term competition. High AVT expression in distinct preoptic regions was found in dominants and subordinates within minutes of encountering each other. During long-term competition, AVT expression remained high in dominants but not in subordinates.

Aggression- and sex-induced neural activity across vasotocin populations in the brown anole

Activity within the social behavior neural network is modulated by the neuropeptide arginine vasotocin (AVT) and its mammalian homologue arginine vasopressin (AVP). However, central AVT/AVP release causes different behavioral effects across species and social environments. These differences may be due to the activation of different neuronal AVT/AVP populations or to similar activity patterns causing different behavioral outputs. We examined neural activity (assessed as Fos induction) within AVT neurons in male brown anole lizards (Anolis sagrei) participating in aggressive or sexual encounters. Lizards possess simple amniote nervous systems, and their examination provides a comparative framework to complement avian and mammalian studies. In accordance with findings in other species, AVT neurons in the anole paraventricular nucleus (PVN) were activated during aggressive encounters; but unlike in other species, a positive correlation was found between aggression levels and activation. Activation of AVT neurons within the supraoptic nucleus (SON) occurred nonspecifically with participation in either aggressive or sexual encounters. Activation of AVT neurons in the preoptic area (POA) and bed nucleus of the stria terminalis (BNST) was associated with engagement in sexual behaviors. The above findings are congruent with neural activation patterns observed in other species, even when the behavioral outputs (i.e., aggression level) differed. However, aggressive encounters also increased activation of AVT neurons in the BNST, which is incongruous with findings in other species. Thus, some species differences involve the encoding of social stimuli as different neural activation patterns within the AVT/AVP network, whereas other behavioral differences arise downstream of this system.

Neuropeptides isotocin and arginine vasotocin in urophysis of three fish species

In this study, for the first time, both neuropeptides isotocin (IT) and arginine vasotocin (AVT) have been identified and measured in urophysis, the neurohaemal organ of the caudal neurosecretory system of teleost fish. So far, AVT, but not IT, was quantified by radioimmunoassay (RIA) in urophysis of several fish species. We have used high-performance liquid chromatographic assay with fluorescence detection (HPLC-FL) preceded by solid-phase extraction (SPE) and liquid chromatography-electrospray ionization triple-quadrupole tandem mass spectrometry (LC-ESI MS/MS) technique to determine both neuropeptides in urophysis of three fish species. The efficiency of peptide’s SPE extraction was 79–85 %. In HPLC-FL method, the limits of detection (LOD) and quantification (LOQ) were estimated as 1.0 and 3.4 pmol/mL for IT and 0.25 and 2.20 pmol/mL for AVT. In LC–MS/MS method, LOD and LOQ were estimated as 0.4 and 1.2 pmol/mL for IT and 0.06 and 0.2 pmol/mL for AVT. The chromatographic methods are good alternative for RIA, because enable to measure both nonapeptides simultaneously in one sample. In round goby (Neogobius melanostomus), three-spined stickleback (Gasterosteus aculeatus) and sea bream (Sparus aurata), urophysial IT concentrations ranged between 0.056 and 0.678 pmol/mg tissue and AVT concentrations ranged between 0.0008 (or even below detection threshold) and 0.084 pmol/mg tissue.

Variation in gene transcript profiles of two V1a-type arginine vasotocin receptors among sexual phases of bluehead wrasse (Thalassoma bifasciatum)

The neurohypophyseal hormone arginine vasotocin (AVT) mediates behavioral and reproductive plasticity in vertebrates, and has been linked to the behavioral changes associated with protogyny in the bluehead wrasse (Thalassoma bifasciatum). In this study, we sequenced full-length cDNAs encoding two distinct V1a-type AVT receptors (v1a1 and v1a2) from the bluehead wrasse, and examined variation in brain and gonadal abundance of these receptor transcripts among sexual phases. End point RT-PCR revealed that v1a1 and v1a2 transcripts varied in tissue distribution, with v1a1 receptor mRNAs at greatest levels in the telencephalon, hypothalamus, optic tectum, cerebellum and testis, and v1a2 receptor transcripts most abundant in the hypothalamus, cerebellum and gills. In the brain, v1a1 and v1a2 mRNAs both localized by in situ hybridization to the dorsal and ventral telencephalon, the preoptic area of the hypothalamus, the ventral hypothalamus and lateral recess of the third ventricle. Quantitative real-time RT-PCR revealed that relative abundance of these two receptor mRNAs varied significantly in brain and gonad with sexual phase. Relative levels of v1a2 mRNAs were greater in whole brain and isolated hypothalamus of terminal phase (TP) male wrasse compared to initial phase (IP) males or females. In the gonad, v1a1 mRNAs were at levels 2.5-fold greater in the testes of IP males – and 4–5-fold greater in the testes of TP males – compared to the ovaries of females. These results provide evidence that V1a-type AVT receptor transcript abundance in the hypothalamus and gonads of bluehead wrasse varies in patterns linked to sexual phase, and bestow a foundation for future studies investigating how differential expression of v1a1 and v1a2 teleost AVT receptors links to behavioral status and gonadal function in fish more broadly.

Hypoosmotic shock adaptation by prolactin involves upregulation of arginine vasotocin and osmotic stress transcription factor 1 mRNA in the cinnamon clownfish Amphiprion melanopus

We cloned cDNA-encoding arginine vasotocin (AVT) from the brain of the cinnamon clownfish Amphiprion melanopus, and that was predicted to encode a protein of 153 amino acids. We examined changes in the expression of AVT mRNA in the brain and arginine vasotocin receptor (AVTR) mRNA and osmotic stress transcription factor 1 (OSTF1) mRNA in the gills of the cinnamon clownfish using quantitative real-time PCR in an osmotically changing environment (seawater (35 psu) → brackish water (BW, 17.5 psu) and BW with prolactin [PRL]). The expression of AVT, AVTR, and OSTF1 mRNA in the brain and gills increased after transfer to BW, and the expression was repressed by PRL treatment. AVT-immunoreactive cells were almost consistently observed in the telencephalon. The plasma Na+ and Cl− levels decreased in BW, but the level of this parameter increased in BW with PRL treatment during salinity change. These results suggest that AVT, AVTR, and OSTF1 play important roles in hormonal regulation in osmoregulation organs, and that PRL improves the hyperosmoregulatory ability of cinnamon clownfish in BW environment.

Arginine-vasotocin expression and participation in reproduction and social behavior in males of the cichlid fish Cichlasoma dimerus

In non-mammalian vertebrates, the nonapeptide arginine-vasotocin (AVT) is involved in the regulation of social behavior related to reproduction and aggression. The cichlid fish Cichlasoma dimerus is a monogamous species with complex social hierarchies. Males are found in one of two basic alternative phenotypes: Non-territorial and territorial males. In this work we characterize the vasotocinergic system in males of C. dimerus in relation to social status with particular emphasis on the various putative sites of action of AVT across the hypothalamic–pituitary–gonad (HPG) axis, and its effects on reproductive and social behavior. The location and distribution of vasotocinergic neurons in the brain was studied, highlighting a morphometric analysis of AVT producing neurons in males of different social status. The effect of AVT on pituitary gonadotropin secretion was analyzed by single pituitary culture while expression of AVT in peripheral organs was studied by RT-PCR using specific primers. Finally, the role of AVT on testicular androgen release was assessed by in vitro incubation of testis. Results showed a positive effect of AVT on gonadotropin secretion, where β-LH showcased a triphasic response under increasing AVT concentration, while β-FSH’s response was dose-dependent and directly proportional. AVT showed a positive and concentration-dependent effect over testicular androgens synthesis and secretion in vitro. Vasotocin expression was observed in testicular somatic tissue located in the interstitial compartment. Thus, the AVT system in C. dimerus appears to be of high complexity, with multiple sites of action in the hypothalamus–pituitary–gonadal axis.

Sexually Dimorphic Effects of Melatonin on Brain Arginine Vasotocin Immunoreactivity in Green Treefrogs (Hyla cinerea)

Arginine vasotocin (AVT) and its mammalian homologue, arginine vasopressin (AVP), regulate a variety of social and reproductive behaviors, often with complex species-, sex- and context-dependent effects. Despite extensive evidence documenting seasonal variation in brain AVT/AVP, relatively few studies have investigated the environmental and/or hormonal factors mediating these seasonal changes. In the present study, we investigated whether the pineal hormone melatonin alters brain AVT immunoreactivity in green treefrogs (Hyla cinerea). Reproductively active male and female frogs were collected during the summer breeding season and a melatonin-filled or blank silastic capsule was surgically implanted subcutaneously. The duration of hormone treatment was 4 weeks, at which time frogs were eutha-nized and the brains and blood collected and processed for AVT immunohistochemistry and steroid hormone assay. We quantified AVT-immunoreactive (AVT-ir) cell bodies in the nucleus accumbens (NAcc), caudal striatum and amygda- la (AMG), anterior preoptic area, suprachiasmatic nucleus (SCN) and infundibular region of the ventral hypothalamus. Sex differences in AVT-ir cell number were observed in all brain regions except in the anterior preoptic area and ventral hypothalamus, with males having more AVT-ir cells than females in the NAcc, amygdala and SCN. Brain AVT was sensitive to melatonin signaling during the breeding season, and the effects of melatonin varied significantly with both region and sex. Treatment with melatonin decreased AVT immunoreactivity in both the NAcc and SCN in male H. cinerea. In contrast, brain AVT was relatively insensitive to melatonin signaling in females, indicating that the regulation of the AVT/AVP neuropeptide system by melatonin may be sexually dimorphic. Finally, melatonin did not significantly influence testosterone or estradiol concentrations of male or female frogs, respectively, suggesting that the effects of melatonin on AVT immunoreactivity are independent of changes in gonadal sex steroid hormones. Collectively, our results indicate that the AVT/AVP neuronal system may be an important target for melatonin in facilitating seasonal changes in reproductive physiology and social behavior.

Arginine vasotocin activates aggressive calls during paternal care in the Puerto Rican coquí frog, Eleutherodactylus coqui

The Puerto Rican coquí frog, Eleutherodactylus coqui, is a directly developing frog that exhibits male territoriality and paternal behaviors. Male frogs also produce advertisement and aggressive vocalizations or calls. Territorial males emit advertisement calls to delineate territories and attract mates. Paternal males guard and brood the directly developing embryos during embryogenesis and up to five days after hatching; advertisement calling is normally absent or infrequent during paternal care. Territorial and paternal males commonly produce aggressive calls during agonistic situations. The neuropeptide, arginine vasotocin (AVT), has been shown to promote calling in anurans, including E. coqui. The objective of this study was to determine if exogenous AVT promotes calling and territorial behavior in paternal males and if it promotes males to abandon their offspring. Injections (IP) of AVT were given to paternal males immediately before the scotoperiod. Frogs were monitored for at least four hours after the injection and the following morning for calling activity and abandonment of egg clutches. AVT-injected males showed a dramatic and significant increase in aggressive calls compared to control males (saline injections). Exogenous AVT did induce advertisement calling in some paternal males but did not significantly elevate paternal males to territorial status nor did it significantly induce abandonment of eggs/embryos. In conclusion, the type of vocalization that AVT activates in E. coqui depends upon the reproductive state of the male and the social environment that surrounds the male.

The fifth neurohypophysial hormone receptor is structurally related to the V2-type receptor but functionally similar to V1-type receptors

The neurohypophysial peptides of the vasopressin (VP) and oxytocin (OT) families regulate salt and water homeostasis and reproduction through distinct G protein-coupled receptors. The current thinking is that there are four neurohypophysial hormone receptors (V1aR, V1bR, V2R, and OTR) in vertebrates, and their evolutionary history is still debated. We report the identification of a fifth neurohypophysial hormone receptor (V2bR) from the holocephalan elephant fish. This receptor is similar to conventional V2R (V2aR) in sequence, but induced Ca2+ signaling in response to vasotocin (VT), the non-mammalian VP ortholog; such signaling is typical of V1-type receptors. In addition, V1aR, V1bR and OTR were also isolated from the elephant fish. Further screening revealed that orthologous V2bRs are widely distributed throughout the jawed vertebrates, and that the V2bR family is subdivided into two subfamilies: the fish specific type-1, and a type-2 that is characteristically found in tetrapods. Analysis suggested that the mammalian V2bR may have lost its function. Based on molecular phylogenetic, synteny and functional analyses, we propose a new evolutionary history for the neurohypophysial hormone receptors in vertebrates as follows: the first duplication generated V1aR/V1bR/OTR and V2aR/V2bR lineages; after divergence from the V2bR lineage, the V2aRs evolved to use cAMP as a second messenger, while the V2bRs retained the original Ca2+ signaling system. Future studies on the role of V2bR in the brain, heart, kidney and reproductive organs, in which it is highly expressed, will open a new research field in VP/VT physiology and evolution.

Arginine Vasotocin Regulation of Interspecific Cooperative Behaviour in a Cleaner Fish

In an interspecific cooperative context, individuals must be prepared to tolerate close interactive proximity to other species but also need to be able to respond to relevant social stimuli in the most appropriate manner. The neuropeptides vasopressin and oxytocin and their non-mammalian homologues have been implicated in the evolution of sociality and in the regulation of social behaviour across vertebrates. However, little is known about the underlying physiological mechanisms of interspecific cooperative interactions. In interspecific cleaning mutualisms, interactions functionally resemble most intraspecific social interactions. Here we provide the first empirical evidence that arginine vasotocin (AVT), a non-mammalian homologue of arginine vasopressin (AVP), plays a critical role as moderator of interspecific behaviour in the best studied and ubiquitous marine cleaning mutualism involving the Indo-Pacific bluestreak cleaner wrasse Labroides dimidiatus. Exogenous administration of AVT caused a substantial decrease of most interspecific cleaning activities, without similarly affecting the expression of conspecific directed behaviour, which suggests a differential effect of AVT on cleaning behaviour and not a general effect on social behaviour. Furthermore, the AVP-V1a receptor antagonist (manning compound) induced a higher likelihood for cleaners to engage in cleaning interactions and also to increase their levels of dishonesty towards clients. The present findings extend the knowledge of neuropeptide effects on social interactions beyond the study of their influence on conspecific social behaviour. Our evidence demonstrates that AVT pathways might play a pivotal role in the regulation of interspecific cooperative behaviour and conspecific social behaviour among stabilized pairs of cleaner fish. Moreover, our results suggest that the role of AVT as a neurochemical regulator of social behaviour may have been co-opted in the evolution of cooperative behaviour in an interspecific context, a hypothesis that is amenable to further testing on the potential direct central mechanism involved.

Distribution of nonapeptide systems in the forebrain of an African cichlid fish, Astatotilapia burtoni

Nonapeptides and their receptors have important functions in mediating social behavior across vertebrates. Where these nonapeptides are synthesized in the brain has been studied extensively in most vertebrate lineages, yet we know relatively little about the neural distribution of nonapeptide receptors outside of mammals. As nonapeptides play influential roles in behavioral regulation in all vertebrates, including teleost fish, we mapped the distributions of the receptors for arginine vasotocin (AVT; homolog of arginine vasopressin) and isotocin (IST; homolog of oxytocin/mesotocin) throughout the forebrain of Astatotilapia burtoni, an African cichlid fish with behavioral phenotypes that are plastic and reversible based on the immediate social environment. We characterized the distribution of the AVT V1a2 receptor (V1aR) and the IST receptor (ITR) using both immunohistochemistry for protein detection and in situ hybridization for mRNA detection, as well as AVT and IST using immunohistochemistry. Expression of the neuropeptide receptors was widely distributed throughout the fore- and midbrain, including the proposed teleost homologs of the mammalian amygdala complex, striatum, hypothalamus, and ventral tegmental area. We conclude that although the location of nonapeptide synthesis is restricted compared to tetrapod vertebrates, the distribution of nonapeptide receptors is highly conserved across taxa. Our results significantly extend our knowledge of where nonapeptides act in the brains of teleosts to mediate social transitions and behavior.

Sex differences in the expression of vasotocin/isotocin, gonadotropin-releasing hormone, and tyrosine and tryptophan hydroxylase family genes in the medaka brain

In teleost fish, sex differences in several behavioral and physiological traits have been assumed to reflect underlying sex differences in the central expression of neurotransmitter/neuromodulator-related molecules, including vasotocin (VT)/isotocin (IT), gonadotropin-releasing hormone (GnRH), and tyrosine and tryptophan hydroxylases (TH and TPH). However, the sex-dependent expression patterns of these molecules have not been fully characterized in the teleost brain. In the present study, we therefore systematically evaluated sex differences in their expression in the medaka (Oryzias latipes) brain. The most prominent sex difference was observed in vt expression in the nucleus posterior tuberis (NPT) and the posterior part of the nucleus ventral tuberis (NVT) in the hypothalamus, where the expression was completely male-specific. Male-biased expression of gnrh1, tph1, and tph2 was also evident in the supracommissural and posterior nuclei of the ventral telencephalic area (Vs/Vp), medial nucleus of the dorsal telencephalic area (Dm), and thalamic dorsal posterior nucleus (DP), respectively. In contrast, the overall expression levels of it and gnrh3 were higher in the female brain than in the male brain. Equally importantly, no conspicuous sex differences were observed in the expression of gnrh2, th1, and th2, despite several previous reports of their sex-biased expression in the brains of other teleost species. Taken together, these data have uncovered previously unidentified sex differences in the expression of VT/IT, GnRH, and TPH in the teleost brain, which may possibly be relevant to sexual dimorphism in some behavioral and/or physiological traits, and have simultaneously highlighted potential species differences in the roles of these molecules.

Morphofunctional changes in the small intestine epithelium of the frog Rana temporaria in the course of hibernation

The structure and function of small intestine epithelium have been studied in overwintering frogs at various stages of hibernation. Experiments on the in vitro absorption of arginine vasotocin (AVT) have shown that the epithelium ability for absorption of this nonapeptide without hydrolysis persists during hibernation. However, there is a decline in AVT absorption observed in January–February and later on (as compared with that in October–December) which is most pronounced in March–April. Changes in the epithelial structure appear by midwinter and are growing towards spring. In April–May, enterocyte height decreased by 33%, microvilli length by 40%, and number of mitochondria by 57% relative to the values at the beginning of hibernation. The absence of signs of breakdown attests to the adaptive nature of the observed changes. The dynamics of the studied parameters reveals morphological and functional plasticity of small intestinal epithelium in Rana temporaria over the hibernation period.

AVTergic modulation of the agonistic behavior in two species of weakly electric fish with different social strategies

Event Abstract Back to Event AVTergic modulation of the agonistic behavior in two species of weakly electric fish with different social strategies Ana Silva1, 2* and Rossana Perrone1 1 Instituto de Investigaciones Biológicas Clemente Estable, Unidad Bases Neurales de la Conducta, Uruguay 2 Facultad de Ciencias, Laboratorio de Neurociencias, Uruguay Social behaviors in vertebrates are controlled by a complex and conserved brain network. It has been postulated that inter and intraspecific modulations of behavior are correlated with distinctively distributed patterns of this network, which depend on neuroendocrine messengers that integrate extrinsic and intrinsic cues. The hypothalamic neuropeptide arginine-vasotocin (AVT) and its mammalian homologue, vasopressin, are key integrators underlying interspecific, sexual, individual, and social context differences in behavior across vertebrate taxa. Comparative studies of AVT actions between related species with different social structure have been useful for the understanding of brain-behavior relationships. We used two species of weakly electric fish with different social strategies: Gymnotus omarorum, solitary and highly aggressive in intra and intersexual encounters all year round, and Brachyhypopomus gauderio, gregarious and only aggressive in intrasexual encounters during the breeding season. Our previous work showed that AVT produces an increase in the EOD basal rate in B. gauderio but not in G. omarorum, and that AVT is responsible for the nocturnal increase of EOD basal rate observed in the former species during the breeding season. In this study, we explored the effects of IP administration of AVT (1µg/g) and of its antagonist (Manning compound, MC, 2µg/g) in the agonistic behavior of both species. We analyzed dyadic encounters (control, n=10; AVT, n=10; MC, n=6) in independent experiments using experimental protocols in which we could predict the outcome of the conflict: 5-30% size asymmetry in intra and intersexual dyads of non-breeding G. omarorum, same size asymmetry + residency in breeding males of B. gauderio. Thirty minutes prior to the agonistic encounter, we injected AVT (or MC) to the potential winner and saline to the potential loser, and evaluated their effects on conflict resolution, levels of aggression and submission, and dynamics of electric and locomotor displays. AVT and MC administration in B. gauderio had no effect on the agonistic behavior except for their actions on EOD rate: AVT induced and MC blocked the increase of EOD rate observed in winners after conflict resolution. Interestingly, in AVT fights, the intruder significantly increased the emission of electric submission signals (offs) that were only rarely observed in control dyadic encounters. AVT induced no effects on the agonistic behavior of G. omarorum, whereas MC induced a decrease in the aggression levels, in the submission signaling, and prevented the decrease of EOD rate observed in control subordinates after conflict resolution. Taken together, our data indicate that the AVTergic system is involved in the modulation of the agonistic behavior of both species. This modulation shows important interspecific differences that might be crucial in shaping different neural strategies for the control of social behavior. Keywords: Aggression, hypothalamic neuropeptide, Neuromodulation Conference: Tenth International Congress of Neuroethology, College Park. Maryland USA, United States, 5 Aug - 10 Aug, 2012. Presentation Type: Poster Presentation (see alternatives below as well) Topic: Social Behavior Citation: Silva A and Perrone R (2012). AVTergic modulation of the agonistic behavior in two species of weakly electric fish with different social strategies. Conference Abstract: Tenth International Congress of Neuroethology. doi: 10.3389/conf.fnbeh.2012.27.00071 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 13 Mar 2012; Published Online: 07 Jul 2012. * Correspondence: Dr. Ana Silva, Instituto de Investigaciones Biológicas Clemente Estable, Unidad Bases Neurales de la Conducta, Montevideo, 11600, Uruguay, asilva@iibce.edu.uy Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Ana Silva Rossana Perrone Google Ana Silva Rossana Perrone Google Scholar Ana Silva Rossana Perrone PubMed Ana Silva Rossana Perrone Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

The role of AVT in the modulation of the circadian rhythmicity of EOD basal rate in South American weakly electric fish.

The role of AVT in the modulation of the circadian rhythmicity of EOD basal rate in South American weakly electric fish.

Skin surface temperature of broiler chickens is correlated to body core temperature and is indicative of their thermoregulatory status

Extreme thermal conditions may dramatically affect the performance of broilers and other domestic animals, thereby impairing animal welfare and causing economic losses. Although body core temperature is the parameter that best reflects a bird's thermal status, practical and physiological obstacles make it irrelevant as a source of information on the thermal status of commercial flocks. Advances in the technology of infrared thermal imaging have enabled highly accurate, noncontact, and noninvasive measurements of skin surface temperature. Providing that skin surface temperature correlates with body temperature, this technology could enable acquisition of reliable information on the thermal status of animals, thereby improving diagnoses of environmental stress in a flock. This study of broiler chickens found a strong positive correlation between body core temperature and facial surface temperature, as recorded by infrared thermal imaging. The correlation was equally strong at all ages from 8 to 36 d during exposure to acute heat stress with or without proper ventilation and after acclimation to chronic heat exposure. A similar correlation was found by measurements in commercial flocks of broilers. Measurements of blood plasma concentrations of corticosterone, thyroid hormones, and arginine vasotocin confirmed that metabolic activity was low after acclimation to chronic exposure to heat, whereas ventilation was at least as efficient as acclimation in reducing thermal stress but did not impair metabolism. In light of these novel results, commercial benefits of infrared thermal imaging technology are suggested, especially in climate control for commercial poultry flocks. The application of this technique to other domestic animals should be investigated in future experiments.