Areas Of Overgrowth Research Articles

СТРУКТУРЫ ИСПОЛЬЗОВАНИЯ ПАХОТНЫХ ЗЕМЕЛЬ В ЛЕСОСТЕПНОЙ ЗОНЕ БАШКИРСКОГО ЗАУРАЛЬЯ СО ВТОРОЙ ПОЛОВИНЫ 20 ВЕКА

We analyzed modern use of arable lands of 1985 in forest-steppe zone of Post-Ural of the Republic of Bashkortostan. Climate is temperate continental. An average annual temperature is 1.6℃ and an average annual precipitation of 427.9 mm. The hottest month is July with an average temperature of 17.5 and the coldest month is January with an average temperature of -14.8℃. Modern agricultural lands usually are used as a hay (43%). Use can be caused with local cattle breeding as traditional horse breeding. Overgrowth captures by 25% of Pinus silvestris. Scot’s pine is growing better on dry lands because it captures more area of overgrowth.

Transformation of a mammary epithelial cell line by the v-raf and v-mos oncogenes

Defective retroviruses were used to investigate the effects of the oncogenes v-raf and v-mos on the behaviour of mammary epithelial cells. Cultures of the mammary epithelial cell lines #43 and NMuMG infected with either virus showed obvious areas of overgrowing cells, which were not seen when cells were infected with control viruses not containing an oncogene. Several clonal cell lines were derived from the areas of overgrowth on #43 cells. Most of these were tumorigenic on subcutaneous inoculation. In culture, several of the cloned lines showed marked morphological abnormalities, had altered rates of growth and saturation densities, and were able to clone in soft agar. Unexpectedly, one raf-containing and one mos-containing line grew more slowly in culture than did control or uninfected cells. Other lines showed only some of these changes. In the mos-containing lines the level of viral transcript corresponded approximately to the extent of transformation, whereas the raf-containing lines showed no such correlation.

Formation of bridges and large cellular clumps in CHO-cell microcarrier cultures: effects of agitation, dimethyl sulfoxide and calf serum.

We have investigated conditions that inhibit the tendency of CHO K1 cells to form cellular bridges between microcarriers and dense clumps of cellular overgrowth in microcarrier cultures. Microcarrier aggregation by cellular bridge formation was found to occur only during periods of rapid cell growth. The level of microcarrier aggregation decreased with increasing agitation intensity. Dense masses of cellular overgrowth formed inside bridges connecting the microcarriers and in clumps that protruded off the microcarrier surface. To replace cells that were continuously sheared from the microcarriers, cell growth occurred preferentially in areas of overgrowth after confluent microcarriers were maintained in a serum-free medium. This ultimately led to poor surface coverage as bare spots developed on the microcarrier away from the areas of dense cellular overgrowth. The development of bare spots was inhibited when confluent microcarriers were maintained in medium supplemented with 1% serum. The development of cellular overgrowth was inhibited by dimethyl sulfoxide. Thus, maintaining confluent microcarriers in medium supplemented with 1% dimethyl sulfoxide and 1% calf serum resulted in microcarriers that appeared similar to monolayer cultures. There was also a decrease in bridging in cultures supplemented with either 1% calf serum or 1% dimethyl sulfoxide/1% calf serum compared to serum-free cultures.