This study aimed to investigate the characteristic values of the human-seat interface in a normal sitting posture, and to numerically mode the load on the chair seat for the structural design of chairs. The stress distributions and the characteristic values of seat were measured under normal sitting posture by using a human body pressure distribution measurement system considering the effects of gender and body mass index (BMI). The stress distribution on the seat was then numerically modeled using three modeling methods. The observed results and the numerical analysisresults were compared. The results showed that an inverted U-shaped pressure distribution was observed in normal sitting posture. The stress was concentrated on the ischial tuberosity with a maximum value of 0.066 MPa. The ratio of the load on the seat to the gravity of the human body weight was about 65.3%. The numerical model established using the body pressure mapping method was superior to those of the uniform load method and the standard loading pad method in terms of stress distribution, maximum stress, and contact area.