Abstract Objectives This study was aimed at determining the status of androgen receptor overexpression in patients with PCa and also correlating overexpression with Gleason score, patient age, and prostate-specific antigen (PSA). Methods This was a cross-sectional analytical laboratory-based study using retrospectively retrieved formalin-fixed, paraffin-embedded (FFPE) tissue blocks of 93 PCa patients who were diagnosed histologically between January and December 2018. Immunostaining was done using AR antibody. Cases with ≥10% positive cells having staining intensity of +1 and above were considered positive. Confirmation of the histological diagnosis and interpretation of the IHC-stained slides were done by two independent expert pathologists in a blinded manner. The association between AR overexpression and categorical variables was tested using the chi-square test, while continuous variables were tested using the t test with a 95% confidence interval (CI). A P < 0.05 was taken as statistically significant. Results AR overexpression was found in 54.8% (51/93) of the cases in this study. Majority of patients, 73.1% (68/93), had a Gleason score of 8 to 10, whereas a Gleason score of 6 to 7 was found in 26.9% (25/93). The mean Gleason score for the cases was 8.47 ± 1.52. Patients with a low Gleason score (≤7) had a higher proportion of AR overexpression than those with a high Gleason score (>8) and the difference was statistically significant (P = .013). Conclusion The clinical relevance of this study was that AR overexpression is associated with a Gleason score but not with age and prostate-specific antigen (PSA). AR overexpression was an independent predictor of PCa prognosis.