Aqueous Extract Research Articles

The quality control and hypoglycemic effect of Cinnamomi Cortex aqueous extract

The quality control and hypoglycemic effect of Cinnamomi Cortex aqueous extract

Immobilization of Xylanase on ZnO nanoparticles obtained by green synthesis from Eupatorium cannabinum L. and its application in enrichment of fruit juices

Juice production is one of the main components of the nutritional food industry. Therefore, improving fruit juice quality and preventing the formation of negative by-products is important for human health. The role of environmentally friendly, safe and economically more affordable green nanocarriers in improving the quality of fruit juice is increasing day by day. For this purpose, zinc oxide nanoparticles (ZnONPs) were prepared from the aqueous extract of the Eupatorium cannabinum L. using the green synthesis method. After immobilization, structural and morphological characterizations of the prepared ZnONPs and xylanase (Xyl)@ZnONPs were carried out, which confirmed that both the synthesis of ZnONPs and the immobilization of Xyl enzyme on ZnONPs were successfully carried out. The biochemical parameters of Xyl@ZnONPs such as optimum pH and temperature, kinetic parameters, thermal stability, reusability, and storage stability were investigated and compared with free Xyl. After immobilization, the optimum pH of free Xyl shifted from 5.0 to an alkaline pH of 8.0, while no change in the optimum temperature (70 °C) was observed for both enzymes. While the activation energy of free Xyl was 5.56 kJ/mol, it was found to be 7.27 kJ/mol for Xyl@ZnONPs. Xyl@ZnONPs outperformed free Xyl due to its long-term storage stability and reusability. Moreover, Xyl@ZnONPs retained 50 % of its activity after 5 reuses and 87 % of its activity after 4 weeks of storage at room temperature. In addition, a decrease in Km value and an increase in Vmax value were observed after immobilization, which means the increased catalytic activity of the Xyl@ZnONPs. As a result of re-using Xyl@ZnONPs in orange juice for 6 cycles, clarification yield of 66.43 % was obtained under optimized conditions. Overall, this study prepared a suitable, environmentally friendly, and robust carrier support for Xyl immobilization and developed a biocatalyst that will provide high performance in the industrial field.

Biotermiticidal Activity of Jatropha gossypifolia Linn. plant extract against Odontotermes obesus (Isoptera: Termitidae)

Abstract: Termiticidal potential of methanol and aqueous leaves extract of Jatropha gossypifolia Linn. were studied against Odontotermes obesus. The plant leaves were dried, powdered and extracted with methanol and aqueous solvent in soxhlet apparatus for 24 hrs. The 20 termites were exposed to various concentrations from 9.0 to 14.5 mg/ml) of methanol and of aqueous plant extract of Jatropha gossypifolia and percent mortality were recorded after 24 hrs. The termiticidal activity of leaves extract of Jatropha gossypifolia were (LC10 = 9.8220 mg/ml, LD50 = 10.093, LC90 =12.667 mg/ml) in methanol and (LC10 = 11.984, LC50 = 13.195, LC90 =14.531 mg/ml) in aqueous solvent respectively. Results revealed that the mortality was increased with increasing in concentration of the plant extracts. The methanol solvent extract of Jatropha gossypifolia showed higher termiticidal property against Odontotermes obesus. Statistical variance, 95% confidence limits and regression equations are presented.

Green Synthesis of Silver Nanoparticles Using Aqueous Fruit Peel Extract of Citrus aurantifolia: Optimization, Its Characterization and Stability Test

In this present study, silver nanoparticles were synthesized by green biological synthesis method using plant extract from fruit peel of Citrus aurantifolia as reducing agents. All the parameters in the synthesis of silver nanoparticles (AgNPs) were optimized to achieve a better yield, controlled size and stability of the particles. The biosynthesis of silver nanoparticles was monitored via UV-vis spectrophotometer and stability test was done. The resulting UV-Vis spectra of synthesized AgNPs from C. aurantifoliafruit peel extract (CAFPE) showed standard surface plasmon resonance band at 420 nm which indicated the presence of AgNPs. The optimum result was obtained with an optimum concentration at 4 mM AgNO3, leaving in a dark room temperature for 24 h and using a concentration 1:3 ratio (extract: silver nitrate). Moreover, the stability of the CAFPE-AgNPs was also observed after 30 days of synthesis and even up to 10 months, indicating optimization plays major role towards the stability fate of nanoparticles. The FTIR analysis showed possible functional groups of biomolecules that play roles in the bioreduction and capping of silver nanoparticles. In addition, it is believed that these parameters are highly suitable for bulk production of single spherical AgNPs with diameter 29.6- 45.2 nm confirmed via FESEM. Thus, the obtained results clearly suggest that optimization of silver nanoparticles may have important role in attaining a better yield and stability of metal nanoparticles, refraining back to its original structure or particles.

Photocatalytic degradation of methylene blue dye under solar light irradiation by zinc oxide nanoparticles using aqueous extract of Cleome gynandra

A simple green synthetic technique is proposed for the synthesis of zinc oxide nanoparticles (ZnONPs) using the plant extract Cleome gynandra as a bio-template. The morphological, functional and structural characterization of synthesized ZnONPs was investigated using several techniques, such as energy dispersive X-ray analysis (EDX), scanning electron microscopy (SEM), X-ray diffraction (XRD) and UV-visible spectroscopy. The synthesized ZnONPs have a wurtzite hexagonal nanostructure with an average size of 15.20 nm. Furthermore, the photocatalytic degradation of methylene blue (MB) in an aqueous solution was carried out using the synthesized ZnONPs. The photocatalytic activity of ZnONPs was investigated by adjusting physicochemical variables as reaction time, medium pH, precursor concentration, and photocatalyst dosage. All results indicate that ZnONPs produced by the green approach exhibits significant photocatalytic activity against dye molecules.

Prophylactic efficacy of aqueous extract of unripe pawpaw (Carica papaya) fruit on hematological and biochemical parameters in induced hyperbilirubinemia Wistar rats

This research offers a comprehensive examination of hyperbilirubinaemia, characterized by elevated levels of bilirubin in the bloodstream leading to jaundice. It presents a prevalent challenge during the neonatal phase, as bilirubin can accumulate in various body parts resulting in potential neurotoxicity. The discussion encompasses factors influencing bilirubin production, conjugation, and elimination in the organism, along with an overview of the causes and symptoms of hyperbilirubinaemia. The study delves into the potential advantages of utilizing Carica papaya, a tropical fruit, for the prevention of hyperbilirubinaemia. Traditional medical approaches for managing this condition are linked to numerous adverse effects, hence the necessity to develop more affordable, easily accessible, and efficacious drugs with minimal or no side effects. Blood samples were procured and subjected to full blood cell count analysis via an automated hematologic analyzer, while serum samples were utilized for quantifying liver enzymes and bilirubin employing a spectrophotometric technique. Additionally, the weight of visceral organs was determined using a Mettler weighing balance. The outcomes of an experimental study conducted on rodents revealed that pretreatment with Carica papaya extract notably decreased bilirubin and liver enzyme levels in the subjects. This highlights the potential role of Carica papaya in the preventive management of hyperbilirubinaemia, underscoring the imperative for further investigation in this domain. In essence, this research serves as a valuable repository of knowledge for healthcare practitioners and researchers keen on addressing hyperbilirubinaemia.

Antiepileptic and antioxidant effects of aqueous extract of Lippia multiflora Moldenke (Verbenaceae) on pilocarpine-induced seizures in Mus musculus Swiss mice

Epilepsy is a chronic neurological disorder that affects the central nervous system. Approximately 3% of the population at some point in their lives would be affected by epileptic disorders in the world, that is to say nearly 70 million people and therefore about 85% live in developing countries. The objective of this study is to determine the anticonvulsant effects of Lippia multiflora on the animal model of epileptogenesis induced by pilocarpine injection in mice during the acute phase. Mice were treated with different doses of the aqueous extract of L. multiflora or sodium valproate. The anticonvulsant effects were evaluated 24 after the injection of pilocarpine by referring to the Racine scale. Then a daily treatment was done for one week corresponding to the acute stage of the disease. Oxidative stress parameters were measured from brain samples taken on the last day of treatment. The injection of pilocarpine induced the Status epilepticus, which is translated by the appearance of convulsive seizures while the administration of different doses of the aqueous extract of L. multiflora led to a significant decrease of the seizures induced by pilocarpine in mice. In addition, L. multiflora extract restored endogenous levels of oxidative stress markers (MDA and NO) and increased the activity of oxidative stress enzymes (SOD, CAT and GSH). L. multiflora possesses anticonvulsant properties mediated by the involvement of GABAA receptors and antioxidants.

Renoprotective effects of Schoenoplectus tabernaemontani rhizomes aqueous extracts against Adriamycin-induced nephropathy in rats.

In recent years, chronic kidney disease (CKD) has emerged as an increasingly significant issue due to the growing prevalence and high treatment costs. While recorded the positive diuretic effect of Schoenoplectus tabernaemontani, there is a lack of reports on its efficacy in treating CKD. The pharmacological effects and mechanisms of Schoenoplectus tabernaemontani rhizomes aqueous extracts (STE) in CKD were investigated by inducing a rodent model of CKD via injection of Adriamycin (ADR; 7.5 mg/kg) into the tail vein of Wistar rats. In summary, our findings suggest that STE has a beneficial effect on anti-renal fibrosis and can reverse ADR-induced renal injury by suppressing oxidative stress and inflammation. Therefore, STE holds promising potential as a treatment option for CKD.

Exploring the healing power of Pistacia lentiscus stems: insights into extraction methods, polyphenolic composition, and health-promoting activities

ABSTRACT This work aimed to evaluate the effect of different extraction solvents on the polyphenolic content, antioxidant and antibacterial activity of Pistacia lentiscus stems. The results obtained show that the extraction yield depends strongly on the polarity of the solvent and the extraction method. The ethanolic extract had the highest yield in both extraction methods investigated, namely Soxhlet (R = 9.89%) and cold maceration (R = 9.20%). The free radical scavenging activity of the extracts showed that the ethanolic extract had the highest antioxidant activity in both extraction methods with an IC50 = 0.023 mg/mL (cold maceration) and an IC50 = 0.034 mg/ml (Soxhlet). The HPLC analysis of the extracts indicates that gallic acid and catechin are the major phenolic compounds. The FTIR results showed that the shift of the stretching is responsible for O-H and C-H bonding. The GC-MS analysis revealed the presence of stearic acid and palmitic acid methyl ester as main compounds. The bacterial analysis of the extracts showed that the aqueous extract represents the most active one against Staphylococcus aureus and Pseudomonas aeruginosa; on the other hand, no antifungal activity was appreciated. Overall, the results indicate that the investigated extracts might be considered valuable sources of bioactive compounds.

<i>In vitro</i> influence on fibrinolysis parameters of aqueous extracts of mushrooms growing on woody plants

Introduction. Various compounds of mushroom extracts are of interest as a source of new active pharmaceutical substances with antithrombotic activity.Aim. The aim of the work is to determine the potential antithrombotic activity in vitro of aqueous extracts of xylotrophic mushrooms: Lentinula edodes, Pholiota squarrosa, Flammulina velutipes (Curtis) Signer, Kuehneromyces mutabilis, Armillaria cepistipes, Armillaria borealis, Hypholoma capnoides, Lentinellus cochleatus, Léccinum aurantíacum, Pleurotus ostreatus.Materials and methods. The study of fibrinolytic activity of water extracts of mushrooms in vitro was carried out. The effect of test objects on plasminogen activation, on the activity of enzyms of the fibrinolysis system α2-antiplasmin, on the time of euglobulin clot lysis and on the concentration of fibrinogen was evaluated. For the analysis we used reagent kits of SPD «RENAM» and LLC Company «Тechnology-Standard» (Russia).Results and discussion. Pronounced antithrombotic activity is demonstrated by an aqueous extract of L. edodes and fractions of aqueous extract. Extracts of L. edodes affect all studied parameters of fibrinolysis to varying degrees. Caps and stems of P. squarrosa, K. mutabilis, and F. velutipes aqueous extracts and fractions of aqueous extracts also demonstrate antithrombotic (fibrinolytic) activity in vitro, having a statistically significant effect on various parameters of fibrinolysis. A less pronounced effect on fibrinolysis parameters in comparison with the above samples is observed when using an aqueous extract of L. cochleatus marked. A. cepistipes, A. borealis, H. capnoides, L. aurantiacum, P. ostreatus fruiting bodies aqueous extracts do not affect the studied parameters of fibrinolysis and the concentration of fibrinogen.Conclusion. The data obtained make it possible to recommend extracts from L. edodes, F. velutipes, P. squarrosa and K. mutabilis for further study, for example, in order to develop functional food products for cardiovascular diseases or to create antithrombotic drugs.

Nanoarchitectonics of an acetogenin-enriched nanosystem mediated by an aqueous extract of Annona cherimola Mill with anti-inflammatory and proapoptotic activity against HepG2 cell line

For the first time, this study shows the nanoarchitectonic process to obtain an acetogenin-enriched nanosystem (AuNPs-Ac) using an aqueous extract from Annona cherimola Mill (ACM) composed of gold nanoparticles embedded in an organic matrix that acts as stabilizing agent and presents anti-inflammatory activity and cytotoxical effect against HepG2 cell line, promoting apoptosis. The synthesis of AuNPs-Ac was confirmed by x-ray diffraction analysis, showing metallic gold as the only phase, and the scanning transmission microscope showed an organic cap covering the AuNPs-Ac. Fourier-transformed infrared suggests that the organic cap comprises a combination of different annonaceous acetogenins, alkaloids, and phenols by the presence of bands corresponding to aromatic rings and hydroxyl groups. High-Performance Liquid Chromatography has demonstrated the presence of annonacin, a potent acetogenin, in the extract of ACM. An in vitro anti-inflammatory activity of the extract of ACM and the AuNPs-Ac was performed using the albumin denaturation method, showing a nonlinear response, which is better than sodium diclofenac salt in a wide range of concentrations that goes from 200 to 400 μg ml−1 with both samples. The viability assay was studied using trypan blue, treating IMR90 and HepG2 at different concentrations of AuNPs-Ac. The results defined a median lethal dose of 800 μg ml−1 against HepG2 through apoptosis according to the ratio of caspase-cleaved 9/alpha-tubulin evaluated. It was also demonstrated that the nanosystem presents a higher cytotoxic effect on the HepG2 cell line than in IMR90, suggesting a targeted mechanism. In addition, the nanosystem performs better than using only the extract of ACM in the anti-inflammatory or antiproliferative test, attributed to their higher surface area.

Recyclable Ag-Au bimetallic nanoparticles supported on acid functionalized multi walled carbon nanotubes for effective catalytic applications

In the present investigation, in situ green reduction approach is used to uniformly decorate the Ag-Au bimetallic nanoparticles (BNPs) on the surface of acid functionalised multi-walled carbon nanotubes (MWCNTs). The adsorbed Terminalia catappa aqueous leaf extract biopolymers on the surface of MWCNTs can increase the in situ reduction of Ag, Au ions to Ag-Au BNPs and stabilise them which can operate as a capper/stabiliser and reductant agent. X-ray diffraction (XRD), high-resolution transmission electron microscopy (HR-TEM), scanning electron microscopy - energy dispersive x-ray spectroscopy (SEM-EDX), Fourier-transform infrared spectroscopy (FT-IR) and UV–visible spectroscopy techniques were employed to examine the structures, morphologies, composition, chemical bonds and optical properties of the functionalised MWCNTs and the nanohybrid. The results revealed that the spherical T.C-Ag-Au bimetallic nanoparticle with average size 12.4 nm was uniformly distributed on the surface of modified MWCNTs. Finally, evaluation of the catalytic activity of the T.C-Ag-Au BNPs decorated MWCNTs exhibited excellent catalytic performance for completing the reduction of 4-Nitrophenol (4-NP) and degradation of alizarin red (AR) dye at ambient temperature with a great rate constant and the degradation efficiency of 98.7% and 96.4%, respectively. The order of reaction, rate constant, half-life and mechanism of catalytic activity of the T.C-Ag-Au BNPs@COOH-MWCNTs nanohybrid were calculated using the Langmuir–Hinshelwood model. The catalyst can be retained and reapplied eight times without affecting its catalytic performance. The interaction between T.C-Ag-Au BNPs and MWCNTs has a synergistic effect, which is accountable for the enhanced catalytic activity.

Green formulation of copper nanoparticles by Pistacia khinjuk leaf aqueous extract: Introducing a novel chemotherapeutic drug for the treatment of prostate cancer

Abstract Prostate cancer is a cancer prevalent form characterized by serious clinical symptoms. The discovery of a novel supplement or medication for prostate cancer within the realm of chemotherapeutic drugs holds significant value. Our research involved the synthesis of copper nanoparticles (CuNPs) infused with Pistacia khinjuk leaf extract to explore their potential anti-prostate cancer, cytotoxic, and antioxidant properties. The green-formulated CuNPs were characterized by TEM, UV-Vis spectrophotometry, FE-SEM, FTIR. The FE-SEM and TEM findings prove the spherical structure with a size of 10–70 nm. The DPPH assay was carried out to examine the effectiveness of antioxidants. CuNPs exhibited the ability to inhibit 50% of DPPH at 215 µg/mL. To assess the anti-prostate cancer properties of CuNPs, an MTT assay was performed on NCI-H660, DU 145, LNCaP clone FGC, and LNCaP clone FGC-Luc2 cells. CuNPs exhibited remarkable efficacy in inhibiting prostate cancer growth, while demonstrating negligible toxicity towards normal cells. The LNCaP clone FGC cell line displayed the most potent anti-prostate cancer effects of CuNPs. IC50 of CuNPs was 322, 365, 247, and 273 on NCI-H660, DU 145, LNCaP clone FGC, and LNCaP clone FGC-Luc2 prostate cancer cells. CuNPs containing Pistacia khinjuk were found to possess noteworthy anti-prostate carcinoma characteristics and presented excellent antioxidant potentials, as highlighted by this study.

Bio‐yoghurt enriched with Spirulina‐encapsulated pomegranate peel: Impact on some metabolomics, antioxidative, textural and colour properties

As an innovative bio‐dairy product, yoghurt was enriched with Spirulina‐encapsulated aqueous/hydroalcoholic extracts of pomegranate peel (PP). Some metabolomics, antioxidative, textural and colour properties of bio‐yoghurt samples were investigated. The samples with Spirulina (YM) and PP (YPA) had the highest lactic, citric and uric acid. The aroma profile of the samples was affected encapsulation process and storage time. The values on day 1 and day 21 were 17.84–17.04 mg/kg for acetaldehyde, 0.98–0.90 mg/kg for diacetyl and 54.42–46.65 mg/kg for acetoin. The sample with encapsulated PP aqueous extract (YEPA) had the highest TPC (2.97 mg GAE/g), and the sample with PP aqueous extract (YPA) had the highest antioxidant capacity (0.36 mg TE/g). The present study describes that bio‐yoghurt enriched with Spirulina‐encapsulated PP could be considered a good alternative for consumers demanding functional dairy products and a holistic approach to a circular bioeconomy.

Phytosynthesis of CeO2-ZnO Nanocomposites Mediated by Parkia Speciosa Hassk Bark Aqueous Extract as Superior Photocatalyst in Reduction of Nitroaromatic Compound

Phytosynthesis of CeO2-ZnO Nanocomposites Mediated by Parkia Speciosa Hassk Bark Aqueous Extract as Superior Photocatalyst in Reduction of Nitroaromatic Compound

Influence of Cuttlefish-Ink Extract on Canned Golden Seabream (Sparus aurata) Quality

Four different concentrations of an aqueous extract of cuttlefish (Sepia spp.) ink (CI) were introduced, respectively, into the packing medium employed during golden seabream (Sparus aurata) canning. The quality parameters of the resulting canned fish were determined and compared to the initial fish and the control canned muscle. An important effect of the CI concentration introduced in the packing medium was proved. The presence in the packing medium of a relatively low CI concentration (CI-2 batch) led to a lower (p < 0.05) lipid oxidation development (fluorescent compound formation), lower (p < 0.05) changes of colour parameters (L* and a* values), and lower (p < 0.05) trimethylamine values in canned fish when compared to control canned samples. Additionally, the two lowest CI concentrations tested led to higher average values of C22:6ω3, ω3/ω6 ratios, and polyene index. On the contrary, the use of the most concentrated CI extract (CI-4 condition) led to a prooxidant effect (higher fluorescence ratio value). In agreement with environmental sustainability and circular economy requirements, the study can be considered the first approach to a novel and valuable use of the current marine byproduct for the quality enhancement of canned fish. On-coming research focused on the optimisation of the CI-extract concentration is envisaged.

Cystoseira tamariscifolia Aqueous Extract Mitigates Salinity Stress in Tomato Plants by Mediating Their Physiology and Biochemistry

Cystoseira tamariscifolia Aqueous Extract Mitigates Salinity Stress in Tomato Plants by Mediating Their Physiology and Biochemistry

Green synthesis and characterization of silver anchored ZnO nanoparticle as the antimicrobial reinforcement for polylactide films

AbstractThe aqueous extract of endophytic fungi (Periconia sp.) was effectively used as a reducing agent to anchor Ag nanoparticles on the ZnO surface. The XRD, UV‐Vis, SEM‐EDX and TEM analyses were performed to ensure the formation of AgNP@ZnO architecture. TEM analysis confirmed that the 4–20 nm sized Ag nanoparticles were anchored evenly on the surface of ZnO with 16–78 nm in size. The antimicrobial study showed the superior performance of AgNP@ZnO functional nanoparticles than the pristine ZnO against pathogenic bacteria Staphylococcus aureus (gram‐positive) and Escherichia coli (gram‐negative). Hence, AgNP@ZnO functional particles were effectively explored as antimicrobial reinforcement for fabricating composite films using polylactic acid (PLA) as a matrix. The obtained nanocomposite films showed excellent antibacterial activity, which increases with increasing AgNP@ZnO loading.

Protective Effects of Roselle Aqueous Extracts against UV-Induced Damage in Zebrafish Fins

Protective Effects of Roselle Aqueous Extracts against UV-Induced Damage in Zebrafish Fins

Detection of active compounds in Aloe vera leaf extract and their inhibitory effect on Pseudomonas aeruginosa.

In the study, two types of Aloe vera leaf extracts were prepared: aqueous and alcoholic extract. The research was conducted at the Department of Life Sciences, Faculty of Science, Tikrit University. From October to January. The active compounds in these alcoholic extracts were detected as tannins, cladosides, saponins, and flavonoids, all of which gave a positive result. The antibacterial activity of the aqueous and alcoholic extracts were estimated at concentrations ranging from (200-12. 5) mg/ml, where the alcoholic extract gave a high efficacy of (34.5) mm, while the aqueous extract had a lower efficacy (29.6) mm at the same concentration. Microbial tests were evaluated using different combinations of the extracts on Pseudomonas aeruginosa isolates, where the alcoholic extract gave the highest inhibitory efficacy to the bacteria.