Aqueous Fruit Pulp Extract Research Articles

Antioxidant activity and toxicological implications of the aqueous extract of Azanza garckeana fruit pulp in female wistar rats

This study examined the antioxidant activity as well as the safety profile of Azanza garckeana aqueous extract in female Wistar rats. Following the random distribution of 28 female Wistar rats (mean weight = 159.25 ± 3.32 g) into four (4) groups labelled (A-D) containing seven rats (animals) each, a daily administration of 0.5 mL distilled water was orally given to the rats in group A, while the aqueous extract of A. garckeana fruit pulp were given orally at 125, 250 and 500 mg/kg to rats in groups B, C and D respectively for 21 days. Some antioxidant activities as well as kidney and liver function indices were examined on the rat using established methods. All liver function indices assayed for showed no significant (p > 0.05) difference in comparison to the control. The concentration of liver enzyme revealed no significant (p > 0.05) difference in liver alanine aminotransferase, liver aspartate aminotransferase, lactate dehydrogenase and liver alkaline phosphatase at 500 mg/kg in comparison to the control group. Conversely, all kidney functions indices show significant increase (p < 0.05) following extract administration, indicating potential effects on kidney function. Significant decrease (p< 0.05) was observed in the level of kidney and liver reduced glutathione and kidney malondialdehyde while kidney and liver superoxide dismutase as well as liver malondialdehyde significantly increased (p<0.05). Overall, aqueous fruit pulp extract of A. garckeana shows no damaging effect on liver indices at the investigated dosage. However, it may have a significant side effect on the kidney due to the biochemical alterations observed in the kidney function indices at the investigated dosages.

Evaluation of the Antipyretic and Anti-Inflammatory Potential of Aqueous Fruit Pulp Extract of Terminalia bellirica

Many fatal diseases have inflammation and fever as clinical symptoms. NSAIDs are the drug of choice for both of these symptoms. But they cause numerous adverse drug reactions including the gastrointestinal, renal and cardiovascular systems. Herbal medicines are known for their efficacy and lack of adverse reactions. So the search for herbal remedies is always on. Terminalia bellirica fruit pulp is used to alleviate a lot of health conditions. Hence this study looked at the antipyretic and anti-inflammatory activities of aqueous extract of Terminalia bellirica fruit pulp in rodents. The carrageenan-induced paw edema model was utilized to test the plant's acute and subacute anti-inflammatory properties. Baker's yeast-induced pyrexia model was utilized to assess the plant's antipyretic activity. Three different groups were administered the extraction (9 mg/kg, 18 mg/kg, and 36 mg/kg). Positive control and negative control for the anti-inflammatory model were Indomethacin (10 mg/kg) and Gum acacia suspension (3 ml/kg of 1 percent) respectively. Positive control and negative control for the anti-pyretic model were Paracetamol 100 mg/kg and 10 ml/kg of 1 percent Gum acacia suspension, respectively. 18 and 36 mg/kg dose of extraction showed substantial minimization of edema (P≤0.01) in tests of acute anti-inflammatory action. 36 mg/kg dose showed substantial minimization of edema in tests of subacute anti-inflammatory action (P≤0.01). The rectal temperature decreased significantly (P≤0.0001) in all experimental groups in Baker's yeast-induced pyrexia tests. Results revealed that the test drug has considerable action against inflammation and pyrexia in rodents.

Insights into the Invitro Antioxidant, Anti-Inflammatory and Anticancer Activities of Limonia acidissima Fruits

Wood apple botanically identified as Limonia acidissima is an indigenous fruit with amazing nutritional and health benefits. Reports from traditional literature of Ayurveda and Siddha portrays the medicinal properties of this fruit. The scooped pulp of the ripe fruit is consumed as such or it can be made into different recipes. But inclusion of this fruit in our diet is not found as a regular practice. Many people are still unaware of the benefits of this fruit. Hence, this study was taken up to unravel the biological potencies of this fruit by conducting in vitro experiments. Phytochemicals such as alkaloids, flavonoids, phenols, saponins and ascorbic acid have been estimated. Anti-inflammatory activity of the aqueous extract of fruit pulp combined with outer rind has been evaluated through inhibition of albumin denaturation. Among the 5 different concentrations (200, 400, 600, 800, 1000 µg/ml), at 1000 µg/ml wood apple has shown 74.55% of protein denaturation inhibition which was compared with standard Diclofenac sodium. Antioxidant capacity of the extract was expressed as mg/100g ascorbic acid equivalent through phosphomolybdenum assay. Dose dependent increase in the antioxidant activity was observed. About 8 different concentrations of the aqueous extract of L. acidissima were evaluated for their cytotoxic activity on MCF 7 cell line. At a concentration of 1000 µg/ml, the extract has shown 93.43% of cytotoxicity and 6.57% of cell viability. Apoptotic induction was evaluated and confirmed by the formation of DNA ladders through DNA fragmentation assay. GCMS analysis of wood apple fruit pulp and rind revealed the presence of several phytochemicals among which many of them had therapeutic activity reported earlier.

Antioxidant and Anticholinesterase Properties of the Aqueous Extract of Balanites aegyptiaca L. Delile Fruit Pulp on Monosodium Glutamate-Induced Excitotoxicity in Swiss Mice.

Balanites aegyptiaca L. Delile (B. aegyptiaca) is used in traditional medicine for the treatment of memory impairment. This work aims to evaluate the antioxidant and anticholinesterase potential of BA fruit pulp extract on excitotoxicity induced by monosodium glutamate (MSG). MSG was administered 30 minutes after treatment with B. aegyptiaca aqueous fruit pulp extract (50, 125, 250, and 500 mg/kg) and vitamin C (100 mg/kg) for 30 days. The negative control group received only MSG, while the control group was given distilled water daily. Behavioral tests parameters (using the novel object recognition, Y-maze, and Barnes maze tests), oxidative stress biomarkers (malondialdehyde, superoxide dismutase, and catalase), nitric oxide, and acetylcholinesterase activity and hippocampal architecture were evaluated. Results obtained revealed that different doses of B. aegyptiaca significantly reversed the deleterious effect of MSG on memory. This was displayed by a significant (p < 0.05) increment in the percentage of spontaneous alternation in the Y-maze test and a significant (p < 0.001) increase in discrimination index in novel object recognition observed with 500 mg/kg extract dose. Moreover, the extract (250 and 500 mg/kg doses) significantly (p < 0.001) increased direct search strategy and significantly decreased (p < 0.01) the time taken to find the target hole in the Barnes maze. A modulation of hyperactivity was observed after administration of all extract doses compared to the negative control group in the open arena. Furthermore, the highest dose of the extract caused a significant (p < 0.001) improvement in antioxidant enzymes activity, associated with a significant (p < 0.001) decrement in nitric oxide and malondialdehyde concentrations and a significant (p < 0.01) decrease in acetylcholinesterase activity. Treatment with the extract also restored normal hippocampal cell architecture. B. aegyptiaca fruit pulp extract could thus confer neuroprotection through its antioxidant and anticholinesterase potential.

Aqueous fruit pulp extract of Adansonia digitata (L) protects against lead-acetate-induced hepato-renal damage in rat model

BackgroundAdansonia digitata (L) fruit has a multi-purpose function one among many, is the antioxidant activities of the fruit by preventing oxidative stress. The effect of Adansonia digitata (L) fruit on lead-induced liver and kidney damage is not clear. Hence, the study was aimed to assessed the protective role of Adansonia digitata (L) fruits against lead acetate induced changes in the liver and kidney function test parameters and the histology of both organ in experimental rats. The rats were divided into five groups with five rats each. All the rats were administered with respective assigned treatment once daily for 6 weeks. Rats in groups I were administered with just distil water (2 ml/kg). Rats in groups II were administered with lead acetate (30 mg/kg) while rats in groups III–V were administered Adansonia digitatata (L) fruit extract (250 mg/kg and 500 mg/kg) and Succimer (5 mg/kg) respectively, then additionally challenged with lead acetate (30 mg/kg) immediately after. At the end of the administration, the blood serum from the experimental rats were used for biochemical analysis. Then, the the organs such as the liver and kidney collected for histological study.ResultsRats administered with Lead acetate showed an increase in AST, ALP and ALT as well as increase in urea and creatinine level (p < 0.001), when compared with the control group (group I), where as Adansonia digitatata (L) fruit prevented the effect (upsurge of serum, Urea, Creatinine, AST, ALP and ALT) of lead acetate. Rats administer with only Lead acetate revealed marked liver steatosis and the degeneration of the kidney glomerulus. The Adansonia digitatata (L) fruit extract and Succimer prevented the histological liver steatosis, as well as the degeneration of the glomerulus of the kidney cytoarchitecture.ConclusionThe findings in this study suggest that Adansonia digitata fruits extract has a protective potentials against lead acetate induced liver and kidney toxicity by preventing the upsurge of liver function enzymes and kidney function parameters. Hence, Adansonia digitata fruits can serve as a natural plant agent that can prevent hepato-renal toxicity. Therefore, Adansonia digitata holds future prospects in preclinical framework to ameliorate organs toxicity for oral therapeutic applications.

Microscopic assessments of the effect of phoenix dactylifera L. in a rat model of mercury-triggered cerebral M1 changes

Context: Mercury is a widespread environmental and industrial pollutant that exerts toxic effects on vital organs. The cerebrum, composed of cortical areas such as the primary motor cortex (M1), is a vulnerable target of mercury toxicity within the central nervous system. Phoenix dactylifera is used in folk medicine to treat diverse disorders, such as loss of consciousness, memory disturbances, and nervous disorders. Aim: This study microscopically evaluated the neuroprotective effect of aqueous fruit pulp extract of P. dactylifera (AFPD) on mercury-triggered M1 changes in Wistar rats. Materials and Methods: Twenty-four Wistar rats were divided into six groups (I–VI; n = 4). Group I was administered distilled water (2 ml/kg); Group II administered mercuric chloride (MCL, 5 mg/kg); Group III administered Vitamin C (100 mg/kg) + MCL (5 mg/kg); Groups IV, V, and VI were administered AFPD (250 mg/kg, 500 mg/kg, and 1000 mg/kg, respectively) followed by MCL (5 mg/kg). Neuroprotective property was evaluated by microscopic assessment of M1 region applying histological techniques and analysis of histometric features of M1 neurons. Statistical Analysis Used: One-way ANOVA and paired sample t-test were used. Results: Microscopic examination of MCL-treated cerebral sections revealed M1 histoarchitectural distortion and neurodegenerative changes such as pyknosis, neuronal shrinkage, chromatolysis, loss of pyramidal neurites, and altered Nissl substance reactivity, relative to the control. Administration of AFPD remarkably ameliorated MCL-triggered M1 changes, especially at dose 500 mg/kg with neuroprotective property comparable to the reference drug, Vitamin C. Conclusion: AFPD is potentially efficacious in ameliorating mercury-triggered microscopic alterations in M1 region of Wistar rats. The neuroprotective property of AFPD could be attributed to antioxidant properties of constituent phytochemicals.

Effects of aqueous extract of fruit pulp of Adansonia digitata L. on the oxidative stress profile against Trypanosoma brucei brucei infection in albino rats

BackgroundChemotherapy is the most widely used means of controlling trypanosomosis, however, effectiveness of the drugs available is limited by a number of factors. This study investigates the oxidative stress profile of aqueous extract of the fruit pulp of Adansonia digitata on some organs in rats infected with Trypanosoma brucei brucei.MethodsThirty-five male albino rats were divided into 7 groups of 5 rats each. Groups B, C, D, E, F and G were inoculated with 0.20 ml of suspension containing 106 T. b. brucei. Group A were neither infected nor treated. Group B were infected but not treated. At onset of parasitaemia, rats in group C were treated with diminazene aceturate at 3.5 mg/kg body weight once, while rats in group D were treated with vitamin C at 200 mg/kg body weight for 3 days consecutively. Rats in groups E, F and G were treated orally for 3 days with the aqueous extract of fruit pulp of A. digitata at a dosage of 40 mg/kg, 80 mg/kg and 160 mg/kg body weight respectively. Liver and kidney tissues of the rats were collected at necropsy (10 days PI) for oxidative stress analysis.ResultsThere was a significant (p < 0.05) effect in the concentration levels of malondialdehyde, superoxide dismutase, glutathione peroxidase and catalase among the different groups treated with aqueous extract of fruit pulp of A. digitata.ConclusionThe extract of A. digitata exert protective effects against tissue peroxidation in albino rats experimentally infected with T. b. brucei.

&lt;i&gt;In vivo&lt;/i&gt; effect of the aqueous extract of &lt;i&gt;Adansonia digitata&lt;/i&gt; (Linn) fruit pulp on &lt;i&gt;Trypanosoma brucei brucei&lt;/i&gt; infection in Wistar rats

This study investigated the effects of aqueous extract of fruit pulp of Adansonia digitata on albino rats infected with Trypanosoma brucei brucei. Acute toxicity test was conducted on the extract and then analysed for some phytoconstituents. Thirty-five adult rats were divided into seven groups of five rats each. Group A were the non-infected control group while groups B, C, D, E, F and G were inoculated with 1x 106 trypanosomes per 100 g body weight (BW). At day 6 post infection (6 PI), groups C and D were treated with diminazene aceturate and vitamin C at dose rates of 3.5 mg/kg BW intra peritonealy once and 200 mg/kg BW orally for 3 days respectively, while groups E, F, G were orally treated for 3 days with 40, 80, and 160 mg/kg BW of the extract respectively. The rats were monitored for parasitaemia, PCV and body weight. The LD50 of the extract was greater than 9000 mg/kg. The phytochemical analysis revealed 3.51% flavonoid, 0.07% alkaloid, 0.10% saponin and 0.03.0% oxalate in the extract. While there was progressive increase in parasitaemia from day 6 PI in groups E and F throughout the study, parasitaemia decreases and was completely cleared by day 8 and 11 PI in groups C and G respectively. PCV of group A was not significantly different (p&gt;0.05) from that of F and G. There was significant difference (p&lt;0.05) in the PCV of Group A and that of groups B and C and highly significantly different (p&lt;0.01) with that of D and E. Significant (p&lt;0.05) body weight increase of rats in groups D, E, F and G at day 10 PI was observed. Thus, fruit pulp of A. digitata at a dose of 9000 mg/kg was not toxic to rats, and contains active compounds with potential In vivo anti-trypanosoma activity.&#x0D; Keywords: Adansonia digitata, Albino rats, Animal trypanosomosis, Phytochemicals, Trypanosoma brucei brucei

Phytochemical Screening and Hepatoprotective Potential of Aqueous Fruit Pulp Extract of Adansonia digitata against CCL4 Induced Liver Damage in Rats

Aim: The current study was carried out to evaluate the hepatoprotective effects of aqueous extract of Adansonia digitata fruit pulp on carbon tetrachloride (CCl4) induced liver damage in rats.&#x0D; Place and Duration of Study: Department of Biochemistry, Faculty of Basic Medical Sciences, College of Health Science, between November 2017 and January 2018.&#x0D; Methodology: A. digitata fruit pulp was extracted by maceration using water; and a concentration of 100 mg/ml was used. Two doses of the aqueous extract (200 mg/kg and 300 mg/kg) and Livoline (25 mg/kg) were used to investigate their hepatoprotective effects on CCl4-induced hepatotoxicity in rats.&#x0D; Results: The two doses of the plant extract showed dose-dependent hepatoprotective effect on CCl4-induced hepatotoxicity, as evident by the significant reduction (P&lt;0.05) in serum levels of AST, ALT, ALP and bilirubin along with the improved histopathological liver sections compared to CCl4-treated animals.&#x0D; Conclusion: Due to its hepatoprotective potentials, A. digitata extract may be used to develop standard treatment drugs against some liver disorders when it is further evaluated through extensive researches.

Aqueous extract of Tamarindus indica fruit pulp exhibits antihyperglycaemic activity.

Tamarindus indica Linn. (T.indica) is a well-known plant used in traditional medicine. The plant is popular for its antidiabetic activity. However, effect so f its aqueous fruit pulp extract on carbohydrate hydrolyzing enzymes and its glucose uptake potential were not explored. The antidiabetic activity was assessed by in-vitro α-amylase and α-glucosidase inhibitory assays after preliminary phytochemical analysis. MTT assay was carried out to find cytotoxicity. Glucose uptake activity of the extract was carried out using L6 myotubes. The results showed a strong α-amylase inhibitory activity for the fruit pulp extract of T.indica compared to standard acarbose; the IC50 of the fruit pulp extract of T.indica and acarbose was 34.19 µg/ml 34.83µM. The extract also showed moderate α-glucosidase inhibitory activity. IC50 of the fruit pulp extract of T.indica and acarbose were 56.91µg/ml and 45.69µM respectively. The cytotoxicity assay showed IC50 of >300µg/ml and ≥1000µM for the fruit pulp extract of T.indica and metformin. The extract showed 63.99±0.08% glucose uptake in L6 myotubes whereas metformin and insulin at 10µg/ml and 10µM exhibited an uptake of 76.99±0.3% and 84.48±0.45% glucose, respectively. The study revealed that the fruit pulp extract of T.indica Linn does not show any cytotoxic effect and has very good α-amylase and good α-glucosidase inhibitory activities. The glucose uptake potential proves its postprandial hypoglycemic effect. Hence, it may be considered an antidiabetic agent for control of postprandial hyperglycemia.

Aqueous Extract Of Fruit Pulp Of &lt;i&gt;Adansonia digitata&lt;/i&gt; (Linn): Phytochemical Screening And &lt;i&gt;In Vitro&lt;/i&gt; Antitrypanosomal Effect

Chemotherapy is the most widely used means of controlling Trypanosomosis, a major health problem to man and his livestock over much of Tropical Africa. However, effectiveness of the drugs available is limited by a number of factors which include increasing parasite resistance, treatment failures and unacceptable toxicity. This study investigated the phytoconstituents of aqueous extract of fruit pulp of Adansonia digitata and its in vitro anti-trypanosomal effects on Federe strain of Trypansoma brucei brucei. Qualitative phytochemical analysis of the extract was carried out using standard technique. While in the in vitro study, about 3 ×105 T. brucei brucei in 0.3mls of blood suspended in 0.4mls Ringer’s solution were each dispensed into tubes (A-D) containing 0.3mls of the aqueous extract at concentrations of 0.02mg/ml, 0.2mg/ml, 2mg/ml and 20mg/ml respectively. The fifth tube (E) was an untreated control (Ringer’s solution and parasite). The tubes were incubated at 370C and examined for the presence and motility of trypanosomes at 15 minutes intervals for 2hours. After the incubation and motility assessment, 0.2ml of the contents of each tube was inoculated intraperitoneally into group of 3 rats, 3 other rats served as uninfected controls. The inoculated animals were then examined daily for the presence of trypanosomes for a period of 60 days. The phytochemical analysis showed the presence of tannins, saponin, phenol, terpenoid, cardiac active glycoside, anthraquinone, reducing sugar, alkaloids, flavonoids and steroids. The extract demonstrated a concentration and time dependent inhibitory effect on trypanosomal motility. Highest effect was observed at concentration of 20mg/ml, with total ceassation of trypanosome motility from 75 minutes of exposure all through the 120 minutes of the incubation. Also rats inoculated with content of the tubes containing the 20mg/ml of the extract did not show parasitaemia and survived the 60 days infectivity test period. However, all rats inoculated with trypanosomes exposed to lower concentrations of the extract showed high parasitaemia with 100% mortality within 5 days post inoculation. Keywords: Adansonia digitata, Trypanosomiasis, Phytochemistry, Trypanosoma brucei brucei.

Anti-inflammatory Effect of the Aqueous Fruit Pulp Extract of Tamarindus indica Linn in Lipopolysaccharide-Stimulated Macrophages

Aim: The aim of the present study was to evaluate the effect of the aqueous fruit pulp extract of Tamarind indica Linn on NO production and iNOS expression in LPS stimulated RAW 264.7 macrophages. Material and Method: The efficacy of tamarind extract on nitric oxide production was determined using RAW macrophages. RT - PCR was used to examine the expression of the iNOS gene in activated macrophages. The Statistical analysis for multiple comparisons was evaluated by one way ANOVA followed by the Dunnett’s test when significant differences were detected. The data were considered to be statistically significant at p < 0.001, p < 0.01 and p < 0.05. Results: LPS stimulated RAW macrophages strongly up regulated the iNOS gene expression levels. The iNOS levels were significantly suppressed in the presence of different concentrations of tamarind extract, compared to LPS treatment alone. The tamarind extract also exhibited dose – dependent decrease in the production of NO. The IC50 was found to be 35.69 μg/ml. LPS stimulated group showed 89.61 ± 0.47 % of NO. Conclusion: Nitric oxide production is found to be more in conditions such as periodontitis, oral squamous cell carcinoma and many other diseases. This study could prove the ability of tamarind fruit pulp extract to inhibit the production of nitric oxide and the iNOS gene expression. Hence, Tamarind indica Linn pulp extract may be used as a good anti-inflammatory agent in periodontitis as well as in conditions associated with over production of nitric oxide in different cancers such as oral squamous cell carcinoma.

Comparative Study of Antioxidant Activities and GC-MS Analysis of Aqueous and Ethanol Fruit Pulp Extract of (Green Kiwi Fruit) Actinidia deliciosa (A.Chev.) C.F. Liang & A.R. Ferguson

The genus name Actinidia refers the Greek word aktinos (rays), which refers to the styles of the female flower, which radiate from the center and resemble the spokes of a wheel. China is the native origin of Kiwi fruit, therefore, it is national fruit of China. It is indigenous to the mountainous regions of southwestern and central china. It is mainly cultivated in Central Europe (New Zealand, Chile, Turkey, Portugal, Italy, Greece, France and Japan), United States and China. The fruits, stems and roots are used as diuretic, febrifuge and sedative. They are used in the treatment of stones in the urinary tract, rheumatoid arthralgia, cancers of the liver and oesophagus. The study aims to evaluate the antioxidant activities and GC-MS analysis of aqueous and ethanol fruit pulp extracts of Actinidia deliciosa. The antioxidant assays such as DPPH˙ radical, Superoxide (O2.‑) radical, ABTS●+ radical cation scavenging activities, phoshomolybdenum reduction and Fe3+ reduction activities were carried out for aqueous and ethanol fruit pulp extracts. The maximum ABTS●+ radical cation scavenging activity for aqueous extract was 84.79±0.46% and ethanol extract was 79.51±0.45% at 30 µg/mL concentration and the IC50 for aqueous extract and ethanol extract was 10.62 and 15.20 μg/mL concentration respectively. The maximum phosphomolybdenum reduction was 92.09±0.28% for aqueous extract and 82.07±0.16% at 120 µg/mL concentration and the RC50 for aqueous extract and ethanol extract was 13.09 and 20.02 μg/mL concentration respectively. GC-MS analysis of aqueous fruit pulp extract showed different active compounds such as Nonanal, Flavone, Phytol with retention time 16.82, 19.92 and 26.73. Also, the GC-MS analysis of ethanol fruit pulp extract possessed various phytocompounds such as Lithic acid, Oleic acid and Vitamin D with retention time 16.15, 18.9 and 20.28. These compounds exhibit antioxidant, hypocholestrolemic, antimicrobial and anticancer activities. Keywords: Actinidiaceae, DPPH˙ radical, Superoxide (O2.‑) radical, ABTS•+ radical cation, Lithic acid, Oleic acid, Phenols.

TAMARIND EXTRACT INHIBITS CYTOCHROME P450 (CYP3A4 ISOZYME) - AN IN VITRO STUDY

Objective: The aim of the present study was to analyze the effect of the aqueous fruit pulp extract of Tamarindus indica L. (tamarind extract) on cytochrome P 450 isoform CYP3A4.Methods: Tamarind extract at different concentrations from 5 to 100 μg/ml was examined for its inhibitory property toward cytochrome P 450 isoform CYP3A4. The various concentrations of tamarind extract, potassium phosphate buffer, CYP450 reagent, and substrate 7-Benzyloxy-4- trifluoromethylcoumarin were added to a 96-well plate. The mixtures were preincubated for 20 min at room temperature. The reaction was started by a mixture of free constituted substrate and NADP+ and incubated at room temperature for 30–60 min. The reaction was stopped by Tris-HCl buffer, pH 10.5. The fluorescent intensities of the products were measured by PerkinElmer Enspire fluorescence reader using an excitation and emission wavelength of 405 nm and 460 nm, respectively. Inhibitory concentration (IC50) was calculated by plotting concentrations of tamarind extract against the corresponding percentage inhibition.Results: All the tested concentrations of extract except 5 μg/ml showed good inhibition against CYP3A4 in a dose-dependent manner. The IC50 value of tamarind for CYP3A4 inhibitory activity was found to be 27.89 μg/ml.Conclusion: T. indica aqueous fruit pulp extract exhibited an inhibitory effect on CYP34A, thereby indicating the possibilities of herb-drug interaction if these extracts are coadministered with the prescribed drugs that are metabolized by CYP3A4.

Antihypercholesterolemic Effects of Fruit Aqueous Extract of Copernicia prunifera (Miller) H. E. Moore in Mice Diet-Induced Hypercholesterolemia.

The present objective of the investigation is to evaluate the antihypercholesterolemic activity of the aqueous fruit pulp extract (APE) of Copernicia prunifera (Miller) H. E. Moore (Arecaceae family). Various chemical characterization methods like thin layer chromatography, Fourier transform infrared spectroscopy, 1H and 13C NMR, and molecular weight by gel permeation chromatography have been employed to characterize the extracted pectin. The present study demonstrated that hypercholesterolemic diet (HD) created hypercholesterolemia, caused significant increases in body weight, total cholesterol, and low-density lipoprotein, and caused decreases in high-density lipoprotein in serum compared with SD group. Two doses (APE 150 and 300 mg/Kg b.w./day) were administered to hyperlipidemic mice for 90 days. APE reversed body weight changes, changed serum lipids to normal values, and significantly inhibited the changes of lipid peroxidation and inflammation in the liver tissues. The renal parameters analyzed (urea and creatinine) altered by diet were reverted to normal values. Our results revealed that aqueous fruit pulp extracts of carnauba reduced hypercholesterolemia showing a potential preventive effect against cardiovascular diseases without side effects cause.

Corrigendum to "Antihypercholesterolemic Effects of Fruit Aqueous Extract of Copernicia prunifera (Miller) H. E. Moore in Mice Diet-Induced Hypercholesterolemia".

[This corrects the article DOI: 10.1155/2017/6376173.].

In vivo assessment of antidiabetic and antioxidative activity of natural phytochemical isolated from fruit-pulp of Eugenia jambolana in streptozotocin-induced diabetic rats

ABSTRACTObjectives: Eugenia jambolana (E. jambolana) is well known for its antidiabetic potential. The aim of the present study was to investigate the antidiabetic and antioxidative effect of an active compound (FIIc) isolated from fruit-pulp of E. jambolana in streptozotocin (45 mg/kg body weight)-induced diabetic rats.Methods: FIIc was isolated from the crude aqueous extract of fruit-pulp by ion-exchange column chromatography and high-performance column chromatography. Detailed UV, NMR, and IR spectra suggested that FIIc is α-hydroxy succinamic acid. FIIc was orally administered to diabetic rats at a dose of 10, 15, and 20 mg/kg body weight (mg/kg bwt.) to determine its effective dose. Thereafter, effective dose was administered to 8 weeks to determine its antidiabetic and antioxidative activity by estimation of glycemic index, lipid profile, key enzymes of carbohydrate metabolism, and oxidative stress parameters.Results: Administration of 15 mg/kg dose daily for 8 weeks led to significant (P < 0.001) fall in fasting blood glucose. Treatment with FIIc (15 mg/kg bwt.) showed significant improvement (P < 0.001) in all the biochemical parameters.Discussion: The results demonstrate that FIIc possesses significant antidiabetic and antioxidative activity.

Evaluation of Diuretic Activity of Aqueous Extract of Ripe Fruit Pulp of Tamarindus indica L. in Rats

Introduction: Diuresis is an important pharmacological property which is useful in many clinical conditions. There is a need of better diuretics with lesser adverse effects in comparison to currently available diuretics. The study aimed to evaluate the diuretic activity of aqueous extract of fruit pulp of Tamarindus indica L. in rats.Methods: The study was undertaken with aqueous extract of fruit pulp of Tamarindus indica in three doses: 300 mg/kg, 600 mg/kg and1200 mg/kg for its diuretic activity in comparison with standard (furosemide) and vehicle control (normal saline) in Wistar rats. Urine volume and electrolytes were measured after 24 hours of drug administration.Results: Aqueous extract of fruit pulp of Tamarindus indica at the dose of 1200 mg/kg exhibited significant diuretic activity (p&lt;0.05) without significant natriuretic effect. Magnesium excretion was also significantly increased in comparison to control group.Conclusion: Aqueous extract of fruit pulp of Tamarindus indica has significant diuretic activity in Wistar rats.

Evaluation Of Anti-Hyperlipidemic Potentials Of Aqueous Fruit Pulp Extract Of Adensonia Digitata In Experimental Rats

Introduction: Hyperlipidemia is a modifiable risk factor of an important killer disease “cardiovascular diseases”, research work exploring anti- hyperlipidemic activity of local plant is timely gesture. Aim: To investigate anti-hyperlipidemic ability of aqueous fruit pulp extract of Adensonia digitata in experimental rats. Methodology: Hyperlipidemia was induced in rats via feeding on high lipid diet (HLD) for 3 weeks. A total of 42 rats used, were divided into seven groups (GI - GVII) of six rats each. GI served as normal control, GII were normal rats administered with only the extract and GIII serves as hyperlipidemic control group. While GIV, GV and GVI were hyperlipidemic group administered with the extract at a dose of 1.25g/kg, 2.5g/kg and 3.75g/kg body weight respectively and GVII were hyperlipidemic given standard hyperlipidemic drug (atorvastatin), the hyperlipidemic rats were on high cholesterol diet throughout the study period. At the end of the first week of extract administration, three animals from each group were randomly selected and sacrificed. At the end of the second week, the remaining three animals from each group were also sacrificed and serum was collected for analysis of serum lipid profile (Total Cholesterol, LDL-Cholesterol, HDL-Cholesterol and Triglyceride). Results: Significant decrease (p&lt; 0.05) in the serum TC, TG, LDL-C, and a significant increase in the HDL-C levels was observed with 1.25g/kg, 2.50g/kg and 3.75g/kg in group IV, V and VI, and group VII treated with Atorvastatin when to compared with the hyperlipidemic control both in the first and second week of treatment. Conclusion: The study demonstrated that A. digitata aqueous fruit pulp extract possesses anti-hyperlipidemic property. The effects may be associated with the phytochemical content of the plant pulp. Further studies on the plant may focus on identification, isolation of active component(s) aimed at formulating anti-hyperlipidemic drugs from natural source.

A Study of the Antihyperlipidaemic and Antioxidant Activities of Aqueous Extract of Fruit Pulp of Cassia Fistula in Albino Rats

The present study was designed to evaluate the antihyperlipidaemic and antioxidant activities of aqueous extract of fruit pulp of Cassia fistula. Aqueous extract was prepared by infusion method and acute oral toxicity tests were performed according to recent OECD guidelines. Hyperlipidaemia was induced in rats by giving high fat diet consisting of coconut oil and vanaspati ghee, in a ratio of 2:3 v/v at a dose of 10 ml/Kg body weight. The extract was given at a dose of 500mg/kgbody weight. For antihyperlipidaemic activity total cholesterol, Triglycerides, HDL Cholesterol and LDL cholesterol was measured, and for antioxidant activity MDA, CAT and SOD were measured using standard methods. The extract showed a significant decrease in total cholesterol, triglycerides, LDL and MDA in blood. On the other hand, HDL, CAT and SOD were increased significantly. The study demonstrates that the fruit pulp extract of Cassia fistula decreases blood lipid levels and lipid peroxidation.