Aquatic Species Invasiveness Screening Kit Research Articles

Potential Invasiveness of Devil Firefish Pterois miles (Bennett 1828) in the Eastern Adriatic Sea

Abstract Devil firefish Pterois miles is an invasive alien species which has severely impacted biodiversity and ecological processes in invaded areas of the Mediterranean. In recent years, its presence has been documented in several countries of the Adriatic Sea. Based on the negative ecological and socio-economic impacts in other areas of the Mediterranean Sea, the potential invasiveness of devil firefish was analyzed for the coastal areas of the three eastern Adriatic countries (Albania, Croatia and Montenegro) using the Aquatic Species Invasiveness Screening Kit. Generally, based on the average value of the Basic Risk Assessment and the threshold used, the outcomes suggest that this species will be highly invasive in the eastern Adriatic countries. In addition, the results have shown that the most affected sector is represented by the local species populations and relative characteristics, while commercial sectors represent the least affected sector. However, considering that the negative impact of non-native species is not seen directly, the governments of these countries should address this issue as soon as possible.

Risk Screening of Invasive Aquatic Species and a Survey of Fish Diversity Using Environmental DNA Metabarcoding Analysis in Shanghai

As the largest coastal city in China, Shanghai’s rapid development in transportation, tourism, trade, and commerce has facilitated the spread and invasion of non-native aquatic organisms. Aquatic organisms are highly elusive, and once established, eradicating them becomes a challenging task. Currently, our understanding of the invasion risk posed by non-native aquatic species in Shanghai is limited. Therefore, it is imperative to investigate the pathways of introduction, distribution, and dispersion and the invasion risk and impacts of non-native aquatic organisms in Shanghai. This study investigated aquatic organisms in Shanghai’s primary water bodies, including Huangpu River, Suzhou River, and Dianshan Lake. The risk assessment was conducted using the Aquatic Species Invasiveness Screening Kit (AS-ISK), and field monitoring was performed with environmental DNA (eDNA) technology. Results of the risk assessment indicate that among the 21 evaluated species, 9 fall into the medium-to-high-risk category with scores ≥26, while 12 are classified as low-risk with scores <26. The top four species with the highest invasion risk are Gambusia affinis, Pomacea canaliculata, Lepomis macrochirus, and Coptodon zillii. This study identified 54 fish species belonging to seven orders, 16 families, and 42 genera at 16 sampling sites in Shanghai, among which Channa maculata, Micropterus salmoides, and Misgurnus bipartitus are non-native. The results suggest that Shanghai faces a high invasion risk of aquatic species, necessitating enhanced scientific prevention and control measures. Early monitoring is essential for species with medium-to-high invasion risk, and a further evaluation and analysis of the risks associated with introduced fish species already present in Shanghai are recommended for aquaculture practices.

Quantification of the invasiveness risk of non‐native macroalgae in the Azores to support conservation measures

Abstract Non‐native species (NNS) might become invasive and threaten biodiversity, economy and public health. Therefore, it is essential that their invasiveness risk be quantified to support conservation measures. The Azores, located in the mid‐northern Atlantic, is the crossroad of macroalgal native distributions and also present almost three times the global ratio of macroalgae reported as NNS. Aquatic Species Invasiveness Screening Kit, a decision‐support tool consisting of 49 questions for the Basic Risk Assessment (BRA) and six for the Climate Change Assessment (CCA), was applied to the 42 marine macroalgae classified as NNS in the Azores. The results indicate that 21 species pose a medium risk of becoming invasive, and 15 present a high risk under current climate conditions (BRA scores only). The combined BRA + CCA scores show that Laurencia dendroidea poses a high risk instead of a medium risk. Thirty‐three Rhodophyta species were screened, with 10 (BRA) and 11 (BRA + CCA) classified as high risk. All four Chlorophyta species ranked as high risk. Only one of the five Ochrophyta species was classified as high risk, namely Rugulopteryx okamurae, one of the most invasive species in European waters. High‐risk species are present in all islands, likely spread by the continuous maritime traffic between islands. Six of the high‐risk species are restricted to single islands (BRA and BRA + CCA scores), and efforts should be made to contain their further expansion. Asparagopsis armata, a species introduced in Europe in the 1920s, is the only high‐risk species present in all islands. Conservation measures for prevention, monitoring and control of NNS are proposed for the different islands of the Azores, including regulation of introduction pathways, prospection of areas with high risk of invasion, risk screening, eradication and containment of NNS. The involvement of stakeholders and maritime workers is also recommended.

Unnoticed introductions, a silent threat for biodiversity: island endemic Ladigesocypris ghigii (Actinopterygii, Leuciscidae) translocated to continental Greece

Morphological similarity between an intruder and a native species can hinder the detection of the intruder. The undetected non-native species will thus have more time to affect the local ecosystem and spread. This paper describes an instance of unnoticed introduction of a fish species morphologically resembling a native endemic species. We report on the first established population of the Rhodes minnow, Ladigesocypris ghigii (Actinopterygii: Leuciscidae), endemic to Rhodes Island, which has been recently introduced to a river near Athens, mainland Greece. When it was first noticed, in 2017, the species was misidentified due to its morphological similarity to Pelasgus marathonicus, the only native leuciscid of the streams and rivers around Athens, until it was subject to genetic and detailed morphological examination. Genetic analyses based on both mitochondrial (cytochrome b) and nuclear (recombination activating gene, RAG1) markers unambiguously identified the collected individuals and provided information on the relationships of Ladigesocypris at both the species and generic level. Our investigation supports that L. ghigii was recently established near Athens, and that the population probably originated from Rhodes Island, an eastern Aegean island. In 2021, a high-density thriving mainland population coexisted solely with the native European eel (Anguilla anguilla) along at least 9.7 km of river habitat. It was assessed as being of high invasive risk for the region where it was established by the Aquatic Species Invasiveness Screening Kit (AS-ISK) screening tool. We propose a feasibility study be undertaken to investigate the application of measures that may include actions to exterminate or contain the population. Special attention is required since the species is considered vulnerable in its native range, while the translocated population coexists with native biota within a stream ecosystem of outstanding local conservation value.

Horizon scanning for potentially invasive non-native marine species to inform trans-boundary conservation management – Example of the northern Gulf of Mexico

Prevention of non-native species introductions and establishment is essential to avoid adverse impacts of invasive species in marine environments. To identify potential new invasive species and inform non-native species management options for the northern Gulf of Mexico (Alabama, Mississippi, Louisiana, Texas), 138 marine species were risk screened for current and future climate conditions using the Aquatic Species Invasiveness Screening Kit. Species were risk-ranked as low, medium, high, and very high risk based on separate (calibrated) thresholds for fishes, tunicates, and invertebrates. In the basic screening, 15 fishes, two tunicates, and 26 invertebrates were classified as high or very high risk under current climate conditions. Whereas, under future climate conditions, 16 fishes, three tunicates, and 33 invertebrates were classified as high or very high risk. Very high risk species included: California scorpionfish Scorpaena guttata, red scorpionfish Scorpaena scrofa, purple whelk Rapana venosa, and Santo Domingo false mussel Mytilopsis sallei under both current and future climates, with weedy scorpionfish Rhinopias frondosa, Papuan scorpionfish Scorpaenopsis papuensis, daggertooth pike conger Muraenesox cinereus, yellowfin scorpionfish Scorpaenopsis neglecta, tassled scorpionfish Scorpaenopsis oxycephalus, brush-clawed shore crab Hemigrapsus takanoi, honeycomb oyster Hyotissa hyotis, carinate rock shell Indothais lacera, and Asian green mussel Perna viridis under climate change conditions only. This study provides evidence to inform trans-boundary management plans across the five Gulf of Mexico states to prevent, detect, and respond rapidly to new species arrivals.

Risk screening of non-native suckermouth armoured catfishes <em>Pterygoplichthys</em> spp. in the River Dinh (Vietnam) using two related decision-support tools

The invasion risk of non-native suckermouth catfishes Pterygoplichthys spp. in the River Dinh (Vietnam) was evaluated using two related decision-support tools, namely the Fish Invasiveness Screening Kit (FISK) and the Aquatic Species Invasiveness Screening Kit (AS-ISK). The results of screenings obtained independently by three assessors using both toolkits were evaluated for consistency, and compared with a screening study previously obtained for Vietnam as a wide risk assessment area. Both FISK and AS-ISK based screenings were shown to provide adequate and potentially important outcomes in terms of risk of invasiveness and were consistent amongst assessors and between toolkits. The invasion risk of Pterygoplichthys in the River Ding was found to be high and the traits of these fishes that influence their success of becoming established were considered. Some aspects in the use of FISK and AS-ISK as decision-support tools as part of the overall risk analysis of species’ invasions for large risk assessment areas are discussed.

Narrowing the Horizon: Using Known Invasives and Propagule Pressure to Focus Risk Screening Efforts on Potential Invasives

About 11 million marine ornamental fish of ca 1800 non-native species are imported into the USA each year. Selecting species for risk assessment is daunting for such a diverse pathway. Herein, we discuss a focused method for species selection: choosing important taxa related to known invaders in high-volume pathways and narrowing prospective species lists to manageable groups of potential hazards. We provide an example using 11 damselfishes, a family with high volume in trade and one of the species established in USA waters. We used a specialized literature review and a risk screening tool (Aquatic Species Invasiveness Screening Kit) to provide an estimate of risk of invasiveness of marine waters of Florida. The established species was identified as a hazard and potential invasive. All other species scored well below the threshold for invasiveness and future climate had little effect on estimates of invasiveness. The analysis revealed little need for additional risk assessment or prohibitions on damselfishes in trade. Education, monitoring, and early detection and rapid response were the main risk management recommendations. The focused species selection process employed herein provided hazard identification and preliminary risk estimates for just 11 species, but collectively, they represent 40% by volume of fishes imported in the marine ornamental trade.

Citizen Science and Expert Judgement: A Cost-Efficient Combination to Monitor and Assess the Invasiveness of Non-Indigenous Fish Escapees

Mapping the distribution and evaluating the impacts of marine non-indigenous species (NIS) are two fundamental tasks for management purposes, yet they are often time consuming and expensive. This case study focuses on the NIS gilthead seabream Sparus aurata escaped from offshore farms in Madeira Island in order to test an innovative, cost-efficient combined approach to risk assessment and georeferenced dispersal data collection. Species invasiveness was screened using the Aquatic Species Invasiveness Screening Kit (AS-ISK), and revealed a high invasion risk. Occurrences of S. aurata were assessed involving citizens in GIS participatory mapping and data from recreational fishing contests. A probability map showed that S. aurata is well dispersed around Madeira Island. This assessment proved to be a cost-efficient early warning method for detecting NIS dispersal, highlighting the urgent need for additional surveys that should search for sexually mature individuals and assess the direct and indirect impacts in the native ecosystem.

Invasiveness assessment of European perch (Perca fluviatilis), pike-perch (Sander lucioperca) and northern pike (Esox lucius) in Albanian freshwater ecosystems by using the Aquatic Species Invasiveness Screening Kit (AS-ISK)

Invasiveness assessment of European perch (Perca fluviatilis), pike-perch (Sander lucioperca) and northern pike (Esox lucius) in Albanian freshwater ecosystems by using the Aquatic Species Invasiveness Screening Kit (AS-ISK)

Providing scientifically defensible evidence and correct calibrated thresholds for risk screening non-native species with second-generation Weed Risk Assessment-type decision-support tools

The risk screening of non-native species that are likely to be invasive in a defined risk assessment area is crucial for implementing strategies of rapid response and mitigation to protect native biodiversity and socio-economic activities. However, for successful risk-ranking of the screened species, scientifically defensible evidence in support of the screening outcomes must be provided, and computation of a correctly calibrated threshold to distinguish between medium-risk and high-risk species must be achieved. This paper reviews published applications of the “second-generation” Weed Risk Assessment-type decision support tools (i.e. the Aquatic Species Invasiveness Screening Kit and the Terrestrial Animal Species Invasiveness Screening Kit) and evaluates them in terms of the above two requirements. Several procedural errors were identified that involved: i) lack of provision of the report with details of the species-specific screenings; ii) incomplete justifications for the responses in the toolkit questionnaire; iii) incomplete details of the protocol used for the a priori categorisation of the screened species for threshold computation; iv) unaccepted or non-existent taxonomic names for the screened species (including typographical errors). Guidelines are provided for both assessors and reviewers to ensure that these procedural errors are avoided in future applications of these risk screening toolkits.

Risk Screening and Distribution of the Invasive Amphipod Dikerogammarus villosus (Sowinsky, 1894) in the River Adda (Northern Italy)

In 2016, Dikerogammarus villosus (Sowinsky, 1894) (Crustacea, Amphipoda) was recorded for the first time in the River Adda, one of the main tributaries of the major Italian river, river Po. Here we investigate its distribution, population density and size classes distribution in the main course of the river, in the territory of the South Adda Regional Park. Furthermore, we defined its level of invasiveness using two different risk screening methods, in order to obtain a more comprehensive evaluation of its potential impacts: AS-ISK (Aquatic Species Invasiveness Screening Kit) and Harmonia+. Finally, we compared the resulting invasiveness level with the output of the GISS (Generic Impact Scoring System) method, also used by IUCN. Our data confirm that the invasive amphipod is well-established in the downstream part of the River Adda, and its distribution seems related to hydrodynamism. Interestingly, its level of invasiveness was evaluated as low by Harmonia+ and high by AS-ISK: this discrepancy is due to different evaluations of environmental and socio-economic impacts of a non-native species carried out by the two methods. Finally, we propose some possible actions to reduce the spreading rate of this invasive amphipod in this area.

Development and application of a multilingual electronic decision-support tool for risk screening non-native terrestrial animals under current and future climate conditions

Electronic decision-support tools are becoming an essential component of government strategies to tackle non-native species invasions. This study describes the development and application of a multilingual electronic decision-support tool for screening terrestrial animals under current and future climate conditions: the Terrestrial Animal Species Invasiveness Screening Kit (TAS-ISK). As an adaptation of the widely employed Aquatic Species Invasiveness Screening Kit (AS-ISK), the TAS-ISK question template inherits from the original Weed Risk Assessment (WRA) and related WRA-type toolkits and complies with the ‘minimum requirements’ for use with the recent European Regulation on invasive alien species of concern. The TAS-ISK consists of 49 basic questions on the species’ biogeographical/historical traits and its biological/ecological interactions, and of 6 additional questions to predict how climate change is likely to influence the risks of introduction, establishment, dispersal and impact of the screened species. Following a description of the main features of this decision-support tool as a turnkey software application and of its graphical user interface with support for 32 languages, sample screenings are provided in different risk assessment areas for one representative species of each of the main taxonomic groups of terrestrial animals supported by the toolkit: mammals, birds, reptiles, amphibians, annelids, insects, molluscs, nematodes, and platyhelminths. The highest-scoring species were the red earthworm Lumbricus rubellus for the Aegean region of Turkey and the New Zealand flatworm Arthurdendyus triangulatus for Croatia. It is anticipated that adoption of this toolkit will mirror that of the worldwide employed AS-ISK, hence allowing to share information and inform decisions for the prevention of entry and/or dispersal of (high-risk) non-native terrestrial animal species – a crucial step to implement early-stage control and eradication measures as part of rapid-response strategies to counteract biological invasions.

Ecological risk screening of sturgeons introduced for aquaculture in Vietnam

AbstractIntroduced non‐native species are major threats to global biodiversity. In Vietnam, non‐native sturgeons have been introduced and widely cultured, but their potential impacts on native aquatic ecosystems have not been screened. Therefore, this study aimed to investigate potential risks that sturgeons likely pose in Vietnam by using the Aquatic Species Invasiveness Screening Kit (AS‐ISK) and interpret outcomes within a management context. Results showed that the Basic Risk Assessment (BRA) scores were 9.0–13.5 and the combined BRA and Climate Change Assessment (BRA+CCA) scores were 5.0–9.5. All five sturgeon species and two hybrid sturgeons cultured in Vietnam were classified as medium risk of invasiveness. Central concerns relating to introduced sturgeons included transmission of new infectious disease agents, and predation and competition for food with native species, especially threatened species. Controlling pathways of sturgeon importation through quarantine processes and preventing sturgeon from escaping into natural waterways are required if sturgeon aquaculture is to be sustainable, native species are to be protected, and biodiversity is to be conserved over the long term.

Invasiveness risks of naked goby, Gobiosoma bosc, to North Sea transitional waters

In recent decades, gobies have dispersed or introduced from the Ponto-Caspian region of eastern Europe in a westerly direction to North American and western European waters. By contrast, the naked goby, Gobiosoma bosc, is the only known gobiid species to have been introduced in an easterly direction from North American to western Europe. The potential invasiveness of G. bosc was assessed using the Aquatic Species Invasiveness Screening Kit (AS-ISK) for rivers and transitional waters for the western and eastern sides of the North Sea. Using globally-derived thresholds, G. bosc was assessed as low-medium invasiveness risk for both sides of the North Sea under current climate conditions. Under future climate conditions, potential invasiveness will increase for both risk assessment areas. Environmental suitability assessment indicated an increase in environmental suitability for G. bosc on the eastern coastline of the North Sea under climate change scenarios and suitability remained unchanged on the western coastline, reflecting the authors' expectations of invasiveness risk.

Biological Invasions in Neotropical Regions: Continental Ichthyofauna and Risk Assessment Protocols.

The objectives of this study were to compare four risk assessment protocols for non-native species in neotropical regions and to assess the potential application of these tools for the management of invasive species and conservation of the ichthyofauna in Brazil. The protocols Fish Invasiveness Screening Kit (FISK), Aquatic Species Invasiveness Screening Kit (AS-ISK), European Non-Native Species in Aquaculture Risk Assessment Scheme (ENSARS) and Fish Invasiveness Screening Test (FIST) were applied for the species Oreochromis niloticus, Coptodon rendalli, Poecilia reticulata and Apteronotus aff. albifrons, whose sources of introduction are aquaculture and fishkeeping. The species were classified as low, medium or high risk of invasion. The scores of the species O. niloticus, C. rendalli and P. reticulata classified them as high risk of invasion in all protocols, whereas A. aff. albifrons had medium risk in the protocols FISK and AS-ISK and low risk in the FIST. Although the results were similar for species whose impacts are widely described, less studied species may have their classification compromised by the lack of evidences in the literature. Despite the difficulties for practical application, the use of these tools may be encouraged, considering the potential threats of other invasive species emerging in Brazil. The comparison between the methods showed that the use of AS-ISK, combined with ENSARS in cases of introductions by aquaculture, provides important answers about ecological impacts on natural environments and about the stages of the aquaculture production chain that should be better inspected.

Invasiveness Risk Assessment of Non-native Freshwater Fish Species (Order: Siluriformes)for Lake Taal, Philippines

Biological invasions caused by the introductions of non-native species (NNS) into their non- native ecosystems have long been a global environmental concern. In the Philippines large-scale importation of non-native freshwater fish species for the aquarium and aquaculture industry have been documented as early as 1940s. However, little invasiveness assessment has been done in the past, which resulted to the invasion of some of these exotic species. Thus, in this paper, 25 NNS (Order: Siluriformes) were assessed using the Aquatic Species Invasiveness Screening Kit (AS-ISK) to determine their potential for becoming invasive in the chosen risk assessment (RA) area, as well as their ecological and socioeconomic potential impacts on the Philippines. Based on the comprehensive ROC Curve, with the use of the Youden-J index, overall threshold values of ≥ 35 for basic risk assessment (BRA) and ≥ 47 for BRA + climate change assessment (CCA) were established to categorize the potential invasiveness of each species as low-, medium-, or high-risk. According to BRA, 10 (40%) NNS were screened as highly invasive, 14 (56%) with medium risk, and one (4%) with low risk. For BRA + CCA, 10 (40%) NNS were categorized as highly invasive, 12 (48%) with medium risk, and three (12%) with low risk. These findings highlight the need for more RAs of the non-native fish species that enter the country through the ornamental industry, as well as improved public educational campaigns and comprehensive regulatory and management policies to prevent their future invasion.

Coupling molecular and risk analysis to investigate the origin, distribution and potential impact of non-native species: an application to ruffe Gymnocephalus cernua in Turkey

Increased detrimental impacts of biological invasions have triggered the development of risk screening tools to streamline the identification of the invasive potential of organisms. These tools assess the biological and historical characteristics of non-native species and predict their invasiveness in a given geographical area. However, challenges for identifying such invasive potential often require a holistic approach, if more accurate predictions are needed. Here, a case study is provided that combines molecular analysis (mtDNA barcoding) with a risk screening (Aquatic Species Invasiveness Screening Kit: AS-ISK) and impact assessment scheme (Environmental Impact Classification for Alien Taxa: EICAT) to address the origin, distribution and potential impact of non-native ruffe Gymnocephalus cernua in Turkey. Recently, several populations of this species have been recorded from the Turkish region of Thrace (the European part of Turkey), which represents the species’ southernmost geographic range of expansion in Europe, where it is non-native. Molecular data suggested the inclusion of populations from Thrace in a haplogroup widespread in eastern and western Europe from where they might have been introduced, as well as natural spread of the species into Thrace through connected river systems rather than by intentional transport. The AS-ISK scores, also accounting for the effects of climate change, indicated that G. cernua is likely to pose a high risk of being invasive in Thrace but a medium risk in Anatolia (the Asian part of Turkey), and the EICAT scores indicated “major” impact. This study suggests that, in the near future, G. cernua has a high potential to invade all suitable habitats in Thrace and establish viable populations in several Turkish freshwater ecosystems, including those in Anatolia.

A Risk Screening of Potential Invasiveness of Alien and Neonative Marine Fishes in the Mediterranean Sea: Implications for Sustainable Management

Biological invasions have posed a major threat to global and regional biodiversity. The Mediterranean Sea, one of the major biodiversity hotspots in the world, has long suffered multiple and frequent invasion events. This paper represents the screening results of the potential invasiveness of 23 introduced marine fish species, which are classified as neonative and alien. To predict the invasiveness potential of species under current and predicted climate conditions, the Aquatic Species Invasiveness Screening Kit (AS-ISK) is applied. Thresholds have been constituted to classify low, medium and high-risk species by receiver operative characteristic curve analysis (ROC). The calibrated basic and climate-change threshold assessment scores used to classify species from low, to medium to high risk were computed between 27.5 and 33.0 respectively. Based on these thresholds, under current climatic conditions, 15 species were high risk, while the remaining species were medium risk, and the Chaetodipterus faber and the Holocentrus adscensionis switched from the medium-risk to the high-risk group under future climatic conditions. The highest score belonged to Fistularia petimba, followed by Siganus fuscescens, Abudefduf spp., Acanthurus monroviae and Lutjanus argentimaculatus. This study focused on the species that have not been assessed for their invasiveness potential, and the results can provide important insights into their sustainable management in the future.

A New Signal of Tropicalization in the Northeast Atlantic: The Spread of the Spotfin Burrfish Chilomycterus reticulatus in Madeira Archipelago and Its Invasion Risk

Current trends in the global climate facilitate the displacement of numerous marine species from their native distribution ranges to higher latitudes when facing warming conditions. In this work, we analyzed occurrences of a circumtropical reef fish, the spotfin burrfish, Chilomycterus reticulatus (Linnaeus, 1958), in the Madeira Archipelago (NE Atlantic) between 1898 and 2021. In addition to available data sources, we performed an online survey to assess the distribution and presence of this species in the Madeira Archipelago, along with other relevant information, such as size class and year of the first sighting. In total, 28 valid participants responded to the online survey, georeferencing 119 C. reticulatus sightings and confirming its presence in all archipelago islands. The invasiveness of the species was screened using the Aquatic Species Invasiveness Screening Kit. Five assessments rated the fish as being of medium risk of establishing a local population and becoming invasive. Current temperature trends might have facilitated multiple sightings of this thermophilic species in the Madeira Archipelago. The present study indicates an increase in C. reticulatus sightings in the region. This underlines the need for updated comprehensive information on species diversity and distribution to support informed management and decisions. The spread of yet another thermophilic species in Madeiran waters provides further evidence of an ongoing tropicalization, emphasizing the need for monitoring programs and the potential of citizen science in complementing such programs.

Impacts Analysis of Alien Macroinvertebrate Species in the Hydrographic System of a Subalpine Lake on the Italian–Swiss Border

The potential invasiveness of alien macroinvertebrate species in the Italian/Swiss hydrographic system of Lake Maggiore (NW Italy) was assessed through the Aquatic Species Invasiveness Screening Kit, a risk assessment tool developed for quantifying the impacts of alien species on the commercial, environmental, and species traits sectors. Data were collected using the databases provided by two regional environmental agencies in northern Italy (Lombardy and Piedmont regions) and by the governmental monitoring program of Switzerland, which were integrated with a systematic literature search on Google scholar and ISI Web of Science. In the assessment area, 16 macroinvertebrate invasive alien species were reported: nine mollusks, four decapods, and three amphipods. The species assessment indicated seven species with a high level of invasiveness: Procambarus clarkii, Faxonius limosus (formerly, Orconectes limosus) and Pacifastacus leniusculus, Dreissena polymorpha, Corbicula fluminea, Sinanodonta woodiana, and Pseudosuccinea columella. The results allow invasive species managers to understand which species to focus their monitoring on in the near future in order to track IAS movements and limit their spread within the hydrographic system and to provide the identification and refinement of concerted bilateral strategies aimed at limiting the impacts of these species. They also account for the implications of future climate change on the invasion potential of each species.