Aquatic Organisms In Surface Water Research Articles

Pesticide Mixtures in Surface Waters of Two Protected Areas in Southwestern Germany

Pesticides enter non-target surface waters as a result of agricultural activities and may reach water bodies in protected areas. We measured in southwestern Germany pesticide concentrations after heavy rainfalls in streams of a drinking water protection area near Hausen (Freiburg) and in the catchment of the Queich (Landau), which originates from the biosphere reserve Palatinate Forest. On average, 32 (n = 21) and 21 (n = 10) pesticides were detected per sample and event in the area of Hausen (n = 56) and in the Queich catchment (n = 17), respectively. The majority of pesticides detected in > 50% of all samples were fungicides, with fluopyram being detected throughout all samples. Aquatic invertebrates exhibited highest risks with 16.1% of samples exceeding mixture toxicity thresholds, whereas risks were lower for aquatic plants (12.9%) and fish (6.5%). Mixture toxicity threshold exceedances indicate adverse ecological effects to occur at half of sites (50%). This study illustrates the presence of pesticide mixtures and highlights ecological risks for aquatic organisms in surface waters of protected areas in Germany.

Occurrence, distribution, and environmental risk of 61 glucocorticoids in surface water of the Yellow River Delta, China

Glucocorticoids (GCs), as important endocrine disrupting compounds and emerging contaminants, could have irreversible adverse effects on aquatic organisms even at ng/L levels. However, previous studies have only focused on the dissolved concentrations of GCs in the water, and limited data are available for their occurrences in the solid phase. In this study, the occurrence, distribution, and environmental risks of 61 natural and synthetic GCs in surface water of the Yellow River Delta (YRD) were simultaneously analyzed by investigating water, suspended particulate matter (SPM) and sediment samples at 64 sites in six major rivers in the wet season. Overall, 51 GCs were detected in all samples from different matrices, and their concentrations were in the range of not detected (ND)−274 ng/L in water, ND−42 ng/g dry weight (dw) in SPM and ND−9.98 ng/g dw in sediment. Natural GCs were the dominant compounds in all samples, followed by synthetic halogenated esters. High concentrations of GCs were observed in discharge outlet samples from livestock farming, aquaculture and industrial production, and the composition differences of GCs between human/animal sources and industrial sources could be used as indicators to identify pollution sources. Most GCs were distributed in the water phase, while compounds with higher log octanol/water partition coefficients (log Kow) tended to be adsorbed to SPM and sediment. The spatial distribution of GCs was primarily affected by anthropogenic activities and hydrodynamic conditions. Four synthetic compounds (budesonide [BD], fluocinolone acetonide [FOA], fluticasone propionate [FP], and clobetasol propionate [CBSP]) were identified as the main contributors to GC activity with a combined contribution of 57 %–95 %. Risk assessment using the risk quotient revealed that low to moderate risks are posed to aquatic organisms in surface water.

A meta-analysis of the occurrence of alkylphenols and alkylphenol ethoxylates in surface waters and sediments in the United States between 2010 and 2020

Nonylphenol (NP), Octylphenol (OP), and their ethoxylates (NPEO and OPEO) have been the subject of considerable scientific and regulatory attention, primarily due to concerns about their aquatic toxicity and endocrine activity. Environmental monitoring has been conducted and reported for these substances in the United States (U.S.) for several decades. This paper develops an updated statistically based meta-analysis of the occurrence and ecological relevance of these substances in fresh and marine surface waters and sediments in the U.S. between 2010 and 2020. The overall objectives of this study were: (1) to evaluate the impact of analytical detection limits (DLs) and treatment of censored or non-detected (ND) samples on reported results, (2) to summarize and evaluate recent (2010–2020) occurrence and concentrations of these substances in surface waters and sediments, (3) to conduct an ecological screening assessment of the potential risks of these substances to aquatic organisms in surface waters and sediments for this same period, and (4) to examine temporal trends of these substances in surface waters and sediments relative to previous investigations. Given that a large proportion of all NP, NPEO, OP and OPEO samples in recent (2010–2019) U.S. monitoring studies were below their respective method Limit of Detection/Limit of Quantification (LOD/LOQ) detection frequency ranging from 0 to 24%), proxy values were imputed using robust regression of order statistics (ROS). Nationally, NP and OP concentrations in fresh surface waters and sediments have decreased from 2010 to 2019. In contrast, changes in NP and OP concentrations in marine waters and sediments were more variable with some increases noted. A screening environmental risk assessment indicated that less than 1% of all samples exceeded U.S. or Canadian environmental quality guidelines. No exceedances were noted after 2016 which indicates a low potential for risk to aquatic organisms.

Assessment of combined algal toxicity of TiO2 nanoparticles and organochlorines in karst surface waters

The widespread use of nanoparticles (NPs) and organic pollutants increases the risk of their coexistence in the aquatic environments. It is uncertain how the combined toxicities of NPs and OCs affect aquatic organisms in surface waters. In this study, the binary combined toxicities of TiO2 NPs with three different organochlorines (OCs)-pentachlorobenzene (PeCB), 3,3,4,4-tetrachlorobiphenyl (PCB-77), and atrazine on Chlorella pyrenoidosa in three karst surface water bodies were investigated. The correlation analysis results indicated that the toxicities of TiO2 NPs and OCs to algae were mainly related to the total organic carbon (TOC) and ionic strength of surface water. Surface water relieved the growth inhibition of the pollutants on algae as compared with ultrapure water (UW). The combined toxic effect caused by the co-exposure of TiO2 NPs-atrazine was synergistic and had an antagonistic effect for TiO2 NPs-PCB-77 in four types of water bodies. However, the co-exposure of TiO2 NPs-PeCB had an additive effect in the Huaxi Reservoir (HX) and synergistic effects in Baihua Lake (BH), Hongfeng Lake (HF), and UW. TiO2 NPs increased the bioaccumulation of OCs by algae. Both PeCB and atrazine significantly increased the bioaccumulation of TiO2 NPs by algae, except for PeCB in HX; however, PCB-77 reduced the bioaccumulation of TiO2 NPs by algae. The toxic effects of TiO2 NPs and OCs on algae in different water bodies were the result of the nature of the pollutants, bioaccumulation, hydrochemical properties, and other factors.

Comprehensive assessment of the ecological risk of exposure to triphenyl phosphate in a bioindicator tadpole

The toxicity of triphenyl phosphate (TPhP) to aquatic organisms in surface waters has been demonstrated; However, an understanding of toxicity profiles of TPhP in amphibians is limited. Therefore, the adverse effects and threshold concentrations of TPhP on metamorphosis, growth, locomotion, and hepatic antioxidants of Gosner stage 25 Polypedates megacephalus tadpoles under long-term (35 d) exposure to six TPhP concentrations until complete metamorphosis were assessed. Additionally, the overall effect of using integrated multiple biomarkers were determined to demonstrate the potential ecological risks of waterborne TPhP at environmentally relevant concentrations in amphibian tadpoles. With increasing TPhP concentrations, physical parameters (snout-vent length, body mass, condition factor, and hepatic somatic index), jumping distance, hepatic catalase, and superoxide dismutase activities decreased, whereas metamorphosis time and malondialdehyde content increased. The threshold concentration of TPhP that affected the tadpole biomarker, except for metamorphosis rate and jumping distance, was 50–400 μg/L. Furthermore, the standardized scores of the examined integrated biomarkers in the six TPhP concentrations were visualized using radar plots and calculated as the integrated biomarker responses (IBRs). The varying TPhP concentrations had different scores in the radar plots, and the threshold for affecting the IBR value was 10 μg/L, which was close to the TPhP concentration in surface waters. Additionally, IBR values were strongly positively correlated with the TPhP concentrations. These findings indicate that environmentally relevant exposure to waterborne TPhP can pose an ecological risk to amphibian tadpoles. This study can serve as a reference and assist in the formulation of relevant policies and strategies to control TPhP pollution in water bodies.

Predicting Metal Bioavailability and Risk of Toxicity in Nigerian Surface Waters: Are the Existing User-Friendly Bioavailability Tools Applicable?

In the present study, we assessed the use of existing user-friendly bioavailability tools to predict metal bioavailability and the risk of toxicity in a typical Nigerian surface water. The effect of prevailing water chemistry on toxicity of metals was also assessed in laboratory studies. Surface water samples were collected at four locations downstream in the Ogun River in Lagos State, and water chemistry analysis was carried out using standard methods. Relevant parameters were inputted into the Bio-Met software and Metal Bioavailability Assessment Tool (M-Bat), which are run on a Microsoft Excel spreadsheet, to obtain the site-specific hazardous concentrations affecting 5% of the population (HC5)/predicted-no-effect concentrations (PNECs)for selected metals and risk characterization ratios (RCRs) at locations downstream in the river. Assessments using the bioavailability tools showed that two locations, Owode-Onirin and Kara, were more sensitive to Zn and Pb inputs compared to the other locations. The RCRs for Cu, Zn, and Pb, respectively, were >1 at all locations, indicating a potential risk of toxicity to aquatic life. Results from laboratory studies conformed with predictions from the bioavailability tools because Zn and Pb were more toxic to aquatic organisms in surface water collected from Owode-Onirin and Kara locations, respectively, compared to toxicity in surface water from other locations. The issue of bioavailability in metal pollution control is widely accepted and has been incorporated into water quality guidelines (WQGs) in the United States, the United Kingdom, and Europe. In the absence of regulations incorporating bioavailability in developing countries including Nigeria, it is essential to assess the applicability of existing methods incorporating bioavailability to surface waters in this region. Findings from such studies will facilitate the development of region-specific WQGs for metals which will be protective of locally relevant aquatic life. Environ Toxicol Chem 2022;41:2537-2547. © 2022 SETAC.

Risk Assessment of the Insecticide Movento 100 SC with the Active Substance Spirotetramat

Movento 100 SC is a new insecticide containing the active substance spirotetramat. The intended use is in stone fruit, pome fruit, vegetables and ornamentals outdoors, and lettuce, tomatoes and cucumbers in greenhouses.
 VKM was requested by the Norwegian Food Safety Authority to consider possible health risk for operators related to the properties of Movento 100 SC; in particular the relevance of the effects of spirotetramat on thyroid hormones, brain, thymus and body weight observed in dogs, and the reproductive effects of spirotetramat observed in rats. VKM was also asked to consider the fate and behaviour of Movento 100 SC with the active ingredient spirotetramat in the environment, and the ecotoxicological effects and risks related to its use. The risk assessment was finalized in a meeting on May 24. 2013, by VKM’s Scientific Panel on Plant Protection Products.
 VKM’s conclusions are as follows:
 Health:
 VKM concludes that spirotetramat shows toxic effects in dogs and rats that could be relevant for humans. Thyroid and thymus glands are target organs in the oral subchronic toxicity studies of spirotetramat in dogs, and effects are observed from 19 mg/kg bw/day (600 ppm). Decreases in circulating thyroid hormone levels were detected in all three studies carried out with dogs (28-, 90-days and 1-year) and should be considered toxicologically relevant. The opinion of the Panel is that it cannot be excluded that the observed brain dilatation in dogs is treatment-related, and relevant to humans.
 Furthermore, VKM concludes that the reproductive effect observed in rats could be relevant for humans.
 VKM proposes a NOAEL of 5 mg/kg bw/day (200 ppm) for spirotetramat based on a 1- year toxicity study in dogs, and a NOAEL of 100 mg/kg bw/day based on the acute neurotoxicity study in rats.
 VKM supports/proposes:
 
 ADI: 0.05 mg/kg bw/day.
 AOEL: 0.05 mg/kg bw/day.
 ARfD: 1 mg/kg bw/day.
 
 Risk calculations show minimal risk if personal protective equipment is used.
 Environment:
 VKM concludes that spirotetramat and its metabolites are not expected to accumulate in soil. It is not expected that spirotetramat or any of its metabolites will reach concentrations in groundwater above the threshold level of 0.1 μg/L when the formulation Movento 100 SC is applied according to the intended use.
 VKM concludes that use of Movento 100 SC with the active substance spirotetramat according to the proposed application scheme in Norway represents a minimal risk of adverse effects on terrestrial mammals, birds, earthworms, and soil microorganisms. However, in-field effects on sensitive species of predatory mites in the crop cannot be excluded.
 The risk of adverse effects on bees is minimal providing that spirotetramat is not used on crops during flowering or when bees are actively foraging.
 For aquatic organisms in surface water, the risk is considered minimal, provided that a 5 m buffer zone to open water is used.

Deriving aquatic life criteria for galaxolide (HHCB) and ecological risk assessment

The polycyclic musk galaxolide (HHCB) is widely used as fragrances in personal care products (PCPs) and has been detected in various environmental media. There is an urgent need to derive aquatic life criteria (ALC) of HHCB for the protection of aquatic organisms. Toxicity tests with 8 Chinese resident aquatic organisms from 3 phyla and 8 families were conducted, and three methods were used for deriving the ALC. A criterion maximum concentration (CMC) of 8.33 μg/L and a criterion continuous concentration (CCC) of 2.20 μg/L were derived according to the USEPA guidelines. The acute predicted no effect concentrations (PNECs) values derived by log-normal species sensitivity distribution (SSD) and log-logistic SSD method were 77.41 and 66.47 μg/L, respectively. In addition, a significant sensitivity difference was observed between the planktonic crustacean and benthic crustacean, and there was no significant difference (p > 0.05) among SSDs based on resident and non-resident species. A comparison of chronic SSDs between HHCB, tonalide (AHTN) and musk ketone (MK) showed that nitro musk (MK) was more toxic to aquatic organisms than polycyclic musks (HHCB and AHTN). Finally, an assessment of risk to aquatic organisms in surface waters and effluents of wastewater treatment plants (WWTPs) worldwide showed that potential risk may exist at several locations. HHCB concentrations in 4.08 and 46.17% of the WWTP effluents in China and 1.71 and 16.13% of the WWTP effluents in other countries exceed the hazard concentration for 5% and 1% aquatic species.

Occurrence, behavior and risk assessment of estrogens in surface water and sediments from Hanjiang River, Central China.

Estrogens have been widely detected in water and might pose a potential threat to the aquatic ecosystem. However, little information is available about the occurrence, multi-phase fate and potential risks of estrogens in Hanjiang River (HR). In this work, the concentration, multi-phase distribution and risk assessment of eight estrogens were studied by investigating surface water and sediment samples from HR during two seasons. These samples were analyzed using the solid-phase extraction (SPE) and liquid chromatography-mass spectrometry (LC-MS). The concentrations of eight estrogens were 4.5-111 ng/l in surface water and 1.7-113 ng/g dry weight in sediments. 4-nonylphenol (NP) was the predominant estrogen in both water and sediments. The estrogens showed significantly spatial variability, with the highest average concentration in the lower section of HR (p < 0.01, F > 12.21). Meanwhile, NP, 17α-estradiol (αE2), Bisphenol A (BPA) and 4-tert-octyphenol (OP) in surface water exhibited higher concentrations in summer than in winter (p < 0.05, F > 4.62). The sediment-water partition coefficients of estrogens suggested that these compounds partitioned more to particulate phase. Risk assessment indicated that estriol (E3) was the main contributor to the total estradiol equivalent concentration. Moreover, estrogen mixtures could pose high ecological risks to aquatic organisms in surface water. Overall, estrogens are ubiquitous in HR, and their potential ecological risks should not be neglected.

Probabilistic assessment of risks of diethylhexyl phthalate (DEHP) in surface waters of China on reproduction of fish

Diethylhexyl phthalate (DEHP) is considered to be an endocrine disruptor, which unlike other chemicals that have either non-specific (e.g., narcotics) or more generalized reactive modes of action, affect the Hypothalamic-pituitary-gonadal (HPG) axis and tend to have specific interactions with particular molecular targets within biochemical pathways. Responding to this challenge, a novel method for deriving predicted no-effect concentration (PNEC) and probabilistic ecological risk assessment (PERAs) for DEHP based on long-term exposure to potentially sensitive species with appropriate apical endpoints was development for protection of Chinese surface waters. PNECs based on potencies to cause lesions in reproductive tissues of fishes, which ranged from 0.04 to 0.20 μg DEHP L−1, were significantly less than those derived based on other endpoints or other taxa, such as invertebrates. An assessment of risks posed by DEHP to aquatic organisms in surface waters of China showed that 88.17% and 78.85% of surface waters in China were predicted to pose risks to reproductive fitness of fishes with thresholds of protection for aquatic organisms based on 5% (HC5) and 10% (HC10), respectively. Assessment of risks of effects based on effects mediated by the HPG-axis should consider effects on chronic, non-lethal endpoints for specific taxa, especially for reproductive fitness of fishes.

Probabilistic modeling of the flows and environmental risks of nano-silica

Nano-silica, the engineered nanomaterial with one of the largest production volumes, has a wide range of applications in consumer products and industry. This study aimed to quantify the exposure of nano-silica to the environment and to assess its risk to surface waters. Concentrations were calculated for four environmental (air, soil, surface water, sediments) and two technical compartments (wastewater, solid waste) for the EU and Switzerland using probabilistic material flow modeling. The corresponding median concentration in surface water is predicted to be 0.12μg/l in the EU (0.053–3.3μg/l, 15/85% quantiles). The concentrations in sediments in the complete sedimentation scenario were found to be the largest among all environmental compartments, with a median annual increase of 0.43mg/kg·y in the EU (0.19–12mg/kg·y, 15/85% quantiles). Moreover, probabilistic species sensitivity distributions (PSSD) were computed and the risk of nano-silica in surface waters was quantified by comparing the predicted environmental concentration (PEC) with the predicted no-effect concentration (PNEC) distribution, which was derived from the cumulative PSSD. This assessment suggests that nano-silica currently poses no risk to aquatic organisms in surface waters. Further investigations are needed to assess the risk of nano-silica in other environmental compartments, which is currently not possible due to a lack of ecotoxicological data.

Ecological Risk of Nonylphenol in China Surface Waters Based on Reproductive Fitness

It has been recognized that ecological risk assessment based on traditional endpoints of toxicity are unable to provide adequate protection because some chemicals may affect reproductive fitness of aquatic organisms at much lower concentrations. In this paper, predicted no effect concentrations (PNECs) for 4-nonylphenol (NP) were derived based either on endpoints of survival, development, and growth or on some nonlethal biomarkers of reproduction, biochemical and molecular biology data. The PNECs derived from reproductive lesion ranged from 0.12 to 0.60 μg NP L(-1), which was significantly lower than those derived from other endpoints. An assessment of ecological risks posed by NP to aquatic organisms in surface waters of China was conducted based on concentration levels of NP in 16 surface waters of 4 major river basins and PNECs derived from reproductive fitness by a tiered ecological risk assessment (ERA). The results showed that 14.2% and 76.5% of surface waters in China may have ecological risks resulting from reproductive fitness if the thresholds of protection for aquatic organisms were set up as 5% (HC5) and 1% (HC1), respectively. The risks were significantly greatest in the Yangtze River Basin than in other major river basins. In comparison with the risks assessed based on traditional endpoints, such as lethality, for those chemicals causing adverse effects on reproduction due to modulation of endocrine function, to be protective of ecosystem structure and function, lesser PNECs, based on sublethal effects of reproduction, were appropriate.

On the relevance of genotoxicity for fish populations II: genotoxic effects in zebrafish ( Danio rerio) exposed to 4-nitroquinoline-1-oxide in a complete life-cycle test

In order to characterize the impact of genotoxic potentials on populations of aquatic organisms in surface waters, zebrafish ( Danio rerio) were exposed to the model genotoxicant 4-nitroquinoline-1-oxide (NQO) in a complete life-cycle test. Fish exposed to mean NQO concentrations of 0, 0.1, 0.3, 1.1, and 2.9 μg/l were examined by several genotoxicity assays with different endpoints. Assays included the unscheduled DNA synthesis (UDS) test, the comet assay, the alkaline filter elution, and the micronucleus test. The genotoxicity assays revealed an increasing genotoxicity, ranging from induction of DNA repair (even at the lowest concentration tested) to primary and secondary DNA alterations at higher concentrations of 1.1 and 2.9 μg/l NQO. Whether the lowered reproductivity observed in the life-cycle test is caused by genotoxic pathways of NQO, remains unclear. However, the results indicate a contradiction to an earlier assumption that genotoxicants as found in the environment are likely to not impact natural populations.

Environmental risk assessment of musk ketone and musk xylene in the Netherlands in accordance with the EU-TGD

An environmental risk assessment has been carried out for musk ketone and musk xylene according to the EU Technical Guidance Document for Environmental Risk Assessment for New and Existing Substances [1]. Musk ketone and musk xylene are used in fragrances for cosmetics and household products. For the fragrance industry these are important fragrance ingredients because of their excellent substantivity as well as for their unique smell, which determines largely the odor of a product. The initial environmental risk assessment is based on information provided by the fragrance industry as represented in the Netherlands by its association NEA, by the Research Institute for Fragrance Materials (RIFM) and data reported in the international open literature. The risk assessment includes an evaluation of the risks for aquatic organisms in surface water and sediment and for soil organisms in soil after application of sewage sludge. Secondary poisoning of fish-eating birds and mammals is considered as well. For each compartment the Predicted Environmental Concentration (PEC) is compared to the Predicted No Effect Concentration (PNEC) to obtain PEC/PNEC ratios. Since monitoring data are available in water, sediment and fish, similar ratios are obtained with measured concentrations instead of the predicted ones. For both substances, PEC/PNEC ratios are at or below 0.1 for organisms in the aquatic environment, including sediment organisms. PEC/PNEC ratios for fish-eating predators are 0.01. Ratios based on monitoring data are below 0.01 for all of these organisms. For soil organisms the PEC/PNEC ratio is 0.5 for musk ketone and 1.3 for musk xylene. Although in the Netherlands (as well as in some other European countries), sewage sludge presently finds no application as fertilizer on agricultural soil, the aim of environmental policy is to upgrade the sludge quality to enable future applications on agricultural and grassland. The reliability of the predicted soil concentrations can be greatly improved by obtaining experimental data on fate and behaviour of musk ketone and musk xylene in digested sludge and soil. The risk assessment provides reassurance for the aquatic compartment while pointing the way for obtaining aditional data for the soil compartment.