Deep soil moisture data have wide applications in fields such as engineering construction and agricultural production. Therefore, achieving the real-time monitoring of deep soil moisture is of significant importance. Current soil monitoring methods face challenges in conducting the large-scale, real-time monitoring of deep soil moisture. This paper innovatively proposes a real-time prediction approach to deep soil moisture combining GNSS-R data and a water movement model in unsaturated soil. This approach, built upon surface soil moisture data retrieved from GNSS-R signal inversion, integrates soil–water characteristics and soil moisture values at a depth of 1 m. By employing a deep soil moisture content prediction model, it provides predictions of soil moisture at depths from 0 to 1 m, thus realizing the large-scale, real-time dynamic monitoring of deep soil moisture. The proposed approach was validated in a study area in Goodwell, Texas County, Oklahoma, USA. Predicted values of soil moisture at a randomly selected location in the study area at depths of 0.1 m, 0.2 m, 0.5 m, and 1 m were compared with ground truth values for the period from 25 October to 19 November 2023. The results indicated that the relative error (δ) was controlled within the range of ±14%. The mean square error (MSE) ranged from 2.90 × 10−5 to 1.88 × 10−4, and the coefficient of determination (R2) ranged from 82.45% to 89.88%, indicating an overall high level of fitting between the predicted values and ground truth data. This validates the feasibility of the proposed approach, which has the potential to play a crucial role in agricultural production, geological disaster management, engineering construction, and heritage site preservation.