Application Of Zinc Oxide Nanoparticles Research Articles

Combined application of zinc oxide and iron nanoparticles enhanced Red Sails lettuce growth and antioxidants enzymes activities while reducing the chromium uptake by plants grown in a Cr-contaminated soil.

Soil contamination with chromium (Cr) is becoming a primary ecological and health concern, specifically in the Kasur and Sialkot regions of Pakistan. The main objective of the current study was to evaluate the impact of foliar application of zinc oxide nanoparticles (ZnO NPs) (0, 25, 50, 100 mg L-1) and Fe NPs (0, 5, 10, 20 mg L-1) in red sails lettuce plants grown in Cr-contaminated soil. Our results showed that both ZnO and Fe NPs improved plant growth, and photosynthetic attributes by minimizing oxidative stress in lettuce plants through the stimulation of antioxidant enzyme activities. At ZnO NPs (100 mgL-1), dry weights of shoots and roots and fresh weights of shoots and roots were improved by 53%, 58%, 34%, and 45%, respectively, as compared to the respective control plants. The Fe NPs treatment (20 mgL-1) increased the dry weight of shoots and the roots and fresh weights of shoots and roots by 53%, 76%, 42%, and 70%, respectively. Application of both NPs reduced the oxidative stress caused by Cr, as evident by the findings of the current study, i.e., at the ZnO NPs (100 mgL-1) and Fe NPs (20 mgL-1), the EL declined by 32% and 44%, respectively, in comparison with respective control plants. Moreover, Fe and ZnO NPs enhanced the Fe and Zn contents in red sails lettuce plants. Application of ZnO NPs at 100 mg L-1 and Fe NPs at 20 mg L-1, improved the Zn and Fe contents in plant leaves by 86%, and 68%, respectively, as compared to the control plants. This showed that the exogenous application of these NPs helped in Zn and Fe fortification in plants. At similar of concenteration ZnO NPs, CAT and APX activities were improved by 52% and 53%, respectively. Similarly, the POD contents were improved by 17% and 45% at 5 and 10 mg/L of Fe NPs. Furthermore, ZnO and Fe NPs limited the Cr uptake by plants, and the concentration of Cr in the leaves of lettuce was under the threshold limit. The exogenous application of ZnO NPs (100 mg L-1) and Fe NPs (20 mg L-1) reduced the Cr uptake in the leaves of red sails lettuce by 57% and 51%, respectively. In conclusion, ZnO and Fe NPs could be used for the improvement of plant growth and biomass as well as nutrient fortification in stressed environments. These findings not only underscore the efficacy of nanoparticle-assisted phytoremediation but also highlight its broader implications for sustainable agriculture and environmental health. However, future studies on other crops with molecular-level investigations are recommended for the validation of the results.

Synergistic enhancement of maize crop yield and nutrient assimilation via zinc oxide nanoparticles and phosphorus fertilization.

Low recovery of conventional fertilizers remains a significant bottleneck for maize production globally. In particular, with phosphate fertilization, zinc (Zn) is prone to precipitation in soil, reducing recovery of both phosphorus (P) and Zn by maize. The present study was designed to investigate the synergistic effect of zinc oxide (ZnO) nanoparticles (NPs) and P on maize crop growth, yield, and nutrient uptake under ZnO seed coating and foliar application in a randomized complete block design. However, plants were subjected to two ZnO NPs levels (0.5 and 12 kg ha-1) amended with two P levels (45 and 90 kg ha-1). ZnO NPs, especially in the form of foliar application, with a P dose of 90 Kg ha-1 significantly (P < 0.05) improved maize crop growth, yield, and nutrient uptake compared with control. In comparison with the control group, plants grown in these conditions absorbed higher levels of Zn and P. Zn uptake rose to 16.34 g ha-1, 137.88 g ha-1, and 166.89 g ha-1 in roots, grains, and stover respectively, and P uptake increased to 0.80 mg kg-1, 10.066 mg kg-1, and 12.17 mg kg-1 respectively. Additionally, seed emergence rate, plant height, and cob length increased by up to 2%, 1177 cm2, and 3.3 cm respectively compared with control. Furthermore, Zn use efficiency was increased up to 38.55% in ZnO NPs foliar application. Application of ZnO NPs at 0.5 kg ha-1 in the form of foliar application with 90 kg ha-1 P dose produced a more pronounced increment in the parameters studied than ZnO NPs seed coating did. © 2024 Society of Chemical Industry.

Soil amendments with nanoparticles control cucumber wilt caused by Fusarium oxysporium

Management of Fusarium wilt, a destructive plant disease, mainly relies on chemicals, but research on novel and eco-friendly methods is needed due to the hazardous effects of chemical pesticides. This study explored the soil application of zinc oxide nanoparticles (ZnONPs) to manage Fusarium wilt disease in cucumber plants. The antifungal activity of ZnONPs against in-vitro growth of Fusarium oxysporum was tested through food poisoning test on the potato dextrose agar medium. The in-vivo potential of NPs for managing Fusarium wilt was checked in pot experiment. In-vitro results confirmed the concentration-dependent in-vitro antifungal activity. The NPs application at 500 µg/ml and 600 µg/ml concentrations showed phytotoxicity and significantly inhibited seed germination and growth of cucumber plants. However, NPs at 300 µg/ml significantly suppressed the pathogenic effects of F. oxysporum on cucumber plants without any phytotoxic effect. The pathogen-inoculated cucumber plants grown in soil amended with NPs showed significantly enhanced growth, lower disease severity, and improved root physiology compared to untreated plants. The plants under NPs treatment showed higher chlorophyll and nitrogen content while decreased membrane permeability and malondialdehyde content. Along with direct antifungal activity, the application of NPs caused an increased expression of defense-related genes and higher activities of defense-related enzymes. These findings verified the efficiency of ZnONPs for suppressing Fusarium wilt disease in cucumber crops, thereby promoting a non-chemical and environmentally friendly approach to controlling Fusarium wilt disease.

A Mini-review on Properties and Applications of Zinc Oxide Nanoparticles

Abstract: In today’s world, nanoparticles play a pivotal role in revolutionizing many industries. Their nano size enables novel applications that have the potential to address pressing global challenges. The paper reviews the major properties and their practical implementations of Zinc Oxide Nanoparticles (ZnO NPs). Different methods of ZnO NP synthesis produced a surface area ranging from 57m2g-1 to 83m2g-1. A precipitation and green synthesis of ZnO NPs demonstrated its catalytic behavior. ZnOx doped with MnCO3 showed the highest catalytic activity. These properties have applications in wastewater treatment and dye removal processes in textile industries. ZnO NPs exhibit UV shielding and photocatalytic properties. ZnO NPs-coated cotton fabric is used in textile industries as it has more UV protection against uncoated cotton fabric. ZnO NPs are major semiconductors having a band gap of 3.34eV. This gives a range of applications in electrical and electronic industries. Biologically synthesized ZnO NP had better anti-microbial properties, which have a wide range of applications in the food industry, compared to chemically synthesized ZnO NP. The anti-cancer properties of ZnO NPs are due to their cytotoxicity making it a potential drug against cancer cells.

Zinc-oxide nanoparticles ameliorated the phytotoxic hazards of cadmium toxicity in maize plants by regulating primary metabolites and antioxidants activity.

Cadmium stress is a major threat to plant growth and survival worldwide. The current study aims to green synthesis, characterization, and application of zinc-oxide nanoparticles to alleviate cadmium stress in maize (Zea mays L.) plants. In this experiment, two cadmium levels (0, 0.6 mM) were applied to check the impact on plant growth attributes, chlorophyll contents, and concentration of various primary metabolites and antioxidants under exogenous treatment of zinc-oxide nanoparticles (25 and 50 mg L-1) in maize seedlings. Tissue sampling was made 21 days after the zinc-oxide nanoparticles application. Our results showed that applying cadmium significantly reduced total chlorophyll and carotenoid contents by 52.87% and 23.31% compared to non-stress. In comparison, it was increased by 53.23%, 68.49% and 9.73%, 37.53% with zinc-oxide nanoparticles 25, 50 mg L-1 application compared with cadmium stress conditions, respectively. At the same time, proline, superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase contents were enhanced in plants treated with cadmium compared to non-treated plants with no foliar application, while it was increased by 12.99 and 23.09%, 23.52 and 35.12%, 27.53 and 36.43%, 14.19 and 24.46%, 14.64 and 37.68% by applying 25 and 50 mg L-1 of zinc-oxide nanoparticles dosages, respectively. In addition, cadmium toxicity also enhanced stress indicators such as malondialdehyde, hydrogen peroxide, and non-enzymatic antioxidants in plant leaves. Overall, the exogenous application of zinc-oxide nanoparticles (25 and 50 mg L-1) significantly alleviated cadmium toxicity in maize. It provides the first evidence that zinc-oxide nanoparticles 25 ~ 50 mg L-1 can be a candidate agricultural strategy for mitigating cadmium stress in cadmium-polluted soils for safe agriculture practice.

Application of ZnO NPs, SiO2 NPs and Date Pollen Extract as Partial Substitutes to Nitrogen, Phosphorus, and Potassium Fertilizers for Sweet Basil Production.

The reduction in mineral fertilizer usage is crucial to the production of medicinal and aromatic products for safety and health purposes. Presently, nanotechnology and the utilization of natural extracts have been extensively studied due to their significant contribution. Ocimum basilicum is commonly employed for various medicinal and aromatic applications. Therefore, randomized complete block design field experiments containing 10 treatments were conducted during the 2021 and 2022 seasons to investigate the effect of nanoparticles (NPs) of ZnO (1.5 and 2.0 g/L) and SiO2 (100 and 150 mg/L) and date palm pollen extract (DPPE) at 10 and 20 g/L either alone or in combination with the ¾ or ½ NPK recommended dose (RD). The NPK RD was served as a control treatment on basil plant production in each season. The effectiveness of ZnO NPs, SiO2 NPs, and DPPE for the decrease in NPK utilization was evaluated. Meanwhile, the most effective treatment for vegetative traits (except for plant height), essential oil %, and yield was ½ NPK RD + 20 g/L DPPE + 2.0 g/L ZnO NPs. Such a treatment increased the branch number/plant, main stem diameter, relevant chlorophyll content, fresh weight/plant, dry weight/plant, essential oil %, and essential oil yield/plant by 21.00 and 9.94%, 58.70 and 40.00%, 20.69 and 15.83%, 68.83 and 58.28%, 48.70 and 56.16%, 45.71 and 35.53%, and 113.22 and 110.32% over the control in the two seasons, respectively. For total phenol and antioxidant activity, the most effective treatments were the ¾ NPK +1.5 g/L ZnO NPs and ½ NPK +2.0 g/L ZnO NPs, respectively. Simultaneously, essential oil composition (with their compound numbers identified (11-29 for control and ¾ NPK RD + 1.5 g/L ZnO NPs)) and the percentage of total compounds, monoterpene hydrocarbons, sesquiterpene hydrocarbons, and oxygenated hydrocarbons were varied among the used applications. The major observed compounds (>8%) estragole, methyl eugenol, linalool, cineole, and caryophyllene were found in different treatments. Thus, the findings of this study indicate the favorable utilization of ZnO NPs, SiO2 NPs, and DPPE in reducing the application of NPK, which may present a novel strategy and beneficial approach.

Alleviation of cadmium-induced oxidative damage through application of zinc oxide nanoparticles and strigolactones in Solanum lycopersicum L.

In this research, zinc oxide nanoparticles (ZnO NPs) are bio-synthesized using Mangifera indica leaf extract and used for reduction of cadmium (Cd) stress in tomato plants.

Green synthesis and Biomedical Applications of Zinc Oxide Nanoparticles. Review

Green synthesis and Biomedical Applications of Zinc Oxide Nanoparticles. Review

Nano-enhanced growth and resilience strategies for Pomegranate cv. Wonderful: Unveiling the impact of zinc and boron nanoparticles on fruit quality and abiotic stress management

The pomegranate fruit is an extremely popular fruit that is grown in various regions around the world. Pomegranate cv. Wonderful confronts severe abiotic stress disorders, like fruit cracking and sunburn, which decrease fruit quality. Nanoparticles offer potential for improving nutrient usage efficiency and lowering undesirable environmental repercussions. During three consecutive stages of development, namely the peak flowering stage, six weeks after peak flowering, and one month prior to harvest, magnificent pomegranate trees were subjected to separate applications of zinc oxide nanoparticles (ZnONPs) and boron oxide nanoparticles (B2O3NPs) at concentrations of 0, 250, 500, and 1000 parts per million (ppm). These treatments were carried out in both the 2021 and 2022 growing seasons. Positive effects were observed at concentrations of 500 and 1000 ppm for ZnONPs and a concentration of 1000 ppm for B2O3NPs on the vegetative growth parameters such as shoot length, leaf area, leaves number per shoot, and canopy volume, besides, leaf chemical characteristics such as leaf chlorophyll concentration and leaf nutrient content such as of nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), zinc (Zn), and boron (B). Spraying with 500 and 1000 ppm B2O3NPs reduces the percentage of fruit sunburn, cracking and increased fruiting percentage such as initial fruit set (%), fruit retention (%), total yield of fruits (kg/tree), and increasing yield (%), while fruit drop % was decreased in comparison to other treatments. In both seasons, T4 (22.37, 22.0 %) and T7 (18.74, 19.490 %) showed an increase in initial fruit set (%). Similarly, there was an increase in fruit retention (%) with T4 (33.08, 34.550 %) and T7 (29.13, 29.430 %) compared to the control. The highest yield increasing percentages were observed with T4 (98.88, 100.270 %) and T3 (66.65, 64.980 %) compared to the control. On the other hand, T4 (8.24, 8.04 %) had the lowest fruit cracking, followed by T7 (12.73, 9.9 %) in the 2021 and 2022 seasons. Similarly, the lowest percentage of sunburned fruit was observed with T4 (20.47, 20.4 %) and T4 (18.43, 16.77 %) in the two seasons compared to the control. In general, our findings indicate that the application of ZnONPs and B2O3NPs on fully bloomed wonderful pomegranate trees, six weeks after full bloom, and one month prior to harvest, resulted in enhanced growth and yield. Additionally, this treatment exhibited a reduction in abiotic stress-related issues, such as fruit cracking and sunburn.

Effects of ZnO/trimethylsilyl cellulose nano-composite coating on anti-UV and anti-fungal properties of papers

Trimethylsilyl cellulose (TMSC) was employed as the coating matrix for the application of zinc oxide nanoparticles (ZnO) onto paper surfaces and the protections of ZnO/TMSC coating against UV-induced damages and fungal spoilage were evaluated. Filter papers were immersed in 2% w/v TMSC solution loaded with ZnO and air-dried. Three ZnO/TMSC suspensions were prepared with 0.1, 0.5, and 1% w/v ZnO NPs. The presences of ZnO/TMSC protective layers were confirmed with ATR-IR spectroscopy. The coated papers exhibited high surface hydrophobicities. After the coated papers were subject to 365-nm UV irradiation at 400 W for 3 h, the contact angles dramatically dropped. The trimethylsilyl (TMS) groups exposed on the surface formed a moisture barrier and were partially removed on UV exposure. ATR-IR revealed that more TMS groups were removed in the protective layer with no ZnO. UV-irradiated papers turned yellow and papers protected with 1% ZnO/TMSC exhibited significantly lower color changes than that of the uncoated one. Compared to the TMSC-coated paper, the addition of ZnO resulted in a significant reduction in tensile strength at maximum. However, after UV irradiation, significant increases in both the strain at break and strength at maximum were only observed in 1% ZnO/TMSC-protected papers. Regarding their anti-fungal properties, the 1% ZnO/TMSC films were effective in growth inhibitions of Aspergillus sp. and Penicillium sp. on the nonirradiated papers. Despite being hydrophilic after UV-irradiation, growths of the molds were severely suppressed on the UV-irradiated paper.

Zinc oxide nanoparticles induce acute lung injury via oxidative stress-mediated mitochondrial damage and NLRP3 inflammasome activation: In vitro and in vivo studies

The widespread application of zinc oxide nanoparticles (ZnO-NPs) brings convenience to our lives while also renders threats to public health and ecological environment. The lung has been recognized as a primary target of ZnO-NPs, however, the detrimental effects and mechanism of ZnO-NPs on the respiratory system have not been thoroughly characterized so far. To investigate the effect of ZnO-NPs on acute lung injury (ALI), Sprague Dawley rats were intratracheally instilled with ZnO-NPs suspension at doses of 1, 2, and 4 mg/kg/day for 3 consecutive days. Our study revealed that ZnO-NPs induced ALI in rats characterized by increased airway resistance, excessive inflammatory response and lung histological damage. In addition, we identified several molecular biomarkers related to the potential mechanism of ZnO-NP-induced ALI, including oxidative stress, mitochondrial damage, and NLRP3 inflammasome activation. The results of in vitro experiments showed that the viability of A549 cells decreased with the increase in ZnO-NPs concentration. Meanwhile, it was also found that ZnO-NP treatment induced the production of ROS, the decrease in mitochondrial membrane potential and activation of NLRP3 inflammasome in A549 cells. Furthermore, to explore the underlying molecular mechanisms of ZnO-NP-induced ALI, N-acetyl-L-cysteine (a ROS scavenger), Cyclosporin A (an inhibitor for mitochondrial depolarization) and Glibenclamide (an inhibitor for NLRP3 inflammasome activity) were used to pre-treat A549 cells before ZnO-NPs stimulation in the in vitro experiments, respectively. The results from this study suggested that ZnO-NP-induced ROS production triggered the accumulation of damaged mitochondria and assembly of NLRP3 inflammatory complex, leading to maturation and release of IL-1β. Moreover, ZnO-NP-induced NLRP3 inflammasome activation was partly mediated by mitochondrial damage. Taken together, our study suggested that ZnO-NPs induced ALI through oxidative stress-mediated mitochondrial damage and NLRP3 inflammasome activation and provided insight into the mechanisms of ZnO-NPs-induced ALI.

Zinc oxide nanoparticles-induced testis damage at single-cell resolution: Depletion of spermatogonia reservoir and disorder of Sertoli cell homeostasis

The widespread application of zinc oxide nanoparticles (ZnO NPs) in our daily life has initiated an enhanced awareness of their biosafety concern. An incredible boom of evidence of organismal disorder has accumulated for ZnO NPs, yet there has been no relevant study at the single-cell level. Here, we profiled > 28,000 single-cell transcriptomes and assayed > 25,000 genes in testicular tissues from two healthy Sprague Dawley (SD) rats and two SD rats orally exposed to ZnO NPs. We identified 10 cell types in the rat testis. ZnO NPs had more deleterious effects on spermatogonia, Sertoli cells, and macrophages than on the other cell types. Cell–cell communication analysis indicated a sharp decrease of interaction intensity for all cell types except macrophages in the ZnO NPs group than in the control group. Interestingly, two distinct maturation states of spermatogonia were detected during pseudotime analysis, and ZnO NPs induced reservoir exhaustion of undifferentiated spermatogonia. Mechanically, ZnO NPs triggered fatty acid accumulation in GC-1 cells through protein kinase B (Akt)/mammalian target of rapamycin (mTOR) signaling and peroxisome proliferator-activated receptor alpha (PPARα)/acyl‐CoA oxidase 1 (Acox1) axis, contributing to cell apoptosis. In terms of Sertoli cells, downregulated genes were highly enriched for tight junction. In vitro and in vivo experiments verified that ZnO NPs disrupted blood-testis barrier formation and growth factors synthesis, which subsequently inhibited the proliferation and induced the apoptosis of spermatogonia. As for the macrophages, ZnO NPs activated oxidative stress of Raw264.7 cells through nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) pathway and promoted cell apoptosis through extracellular signal-regulated kinase (ERK) 1/2 pathway. Collectively, our work reveals the cell type-specific and cellularly heterogenetic mechanism of ZnO NPs-induced testis damage and paves the path for identifying putative biomarkers and therapeutics against this disorder.

Potential effects of spirulina and date palm pollens on zinc oxide nanoparticles -induced hepatoxicity, oxidative stress, and inflammation in male albino rats.

The application of Zinc oxide nanoparticles (ZnO NPs) is substantially growing in industrial products. Therefore, humans are increasingly exposed to ZnO NPs daily due to their extensive range of applications, raising worries about their possible toxicity. In this study, the ameliorative effects of raw Phoenix dactylifera L. (date palm) pollens (DPP) and Spirulina platensis (SP) independently against ZnO NPS-induced hepatoxicity in male albino rats were examined. Six groups (6/group) of adult male albino rats received oral treatment using distilled water (control), SP (1000 mg/kg b. wt.), DPP (100 mg/kg b. wt.), ZnO NPs (100 mg/kg b. wt.), ZnO NPs +SP, and ZnO NPs + DPP respectively for 15 days. The results of the biochemical investigation indicated that the administration of ZnO NPs substantially upregulated (p < 0.05) transaminases, alkaline phosphatase, and bilirubin serum levels. Malondialdehyde and pro-inflammatory cytokine serum levels were also elevated after ZnO NPs administration. Simultaneously, the downregulated catalase and glutathione peroxidase serum activities were significantly suppressed in ZnO NPs treated rats. Moreover, exposure to ZnO NPs induced liver histopathological alterations. The administration of SP and DPP ameliorated the aforementioned effects caused by ZnO NPs. This result can be attributable to the downregulation of hepatic transaminases, alkaline phosphatase, and bilirubin in the serum and the antioxidation system's equilibration, thus alleviating the accumulation of reactive oxygen species. SP and DPP are natural antioxidants with the potential to eliminate inflammation as well as oxidative damage caused by ZnO NPs in hepatic tissue.

Effect of concentration of biosynthesized zinc oxide nanoparticles on the growth and development of Lycopersiconesculentum

The global dissemination of Lycopersicon esculentum, commonly referred to as the tomato, is attributed to its exceptional organoleptic attributes and nutritional contributions across a wide spectrum of culinary creations. Within this context, the progression of research efforts aimed at enhancing the yield of this crop holds paramount importance. In this regard, the application of Zinc Oxide nanoparticles (ZnO NPs) during pivotal growth and developmental phases of L. esculentum emerges as a subject of profound interest. A multitude of investigations have elucidated that zinc, when administered within optimal thresholds, exerts dual roles as both a biofertilizer and a biopesticide, concurrently displaying potential to elevate the nutritional content of consumable fruits, thereby heralding transformative perspectives for conventional agricultural practices. Against this backdrop, this study aims to delineate the nuanced impact of various concentrations of biogenically synthesized ZnO NPs on the growth and maturation trajectory of L. esculentum (tomato). The research trajectory encompassed the synthesis of ZnO NPs and their subsequent characterization. Sequentially, a planned inoculation regimen was orchestrated, comprising six distinct experimental cohorts. Following emergence, a series of assessments were conducted, each spanning a 30-day interval, wherein an array of growth and developmental parameters were meticulously gauged, encompassing variables such as stem elongation, stem thickness, leaf density, floral abundance, cluster profusion, and fruit setting. A judicious amalgamation of empirical data acquisition and statistical scrutiny unveiled discernible differentials among the experimental modalities, significantly signifying the propitious influence of zinc oxide nanoparticles on the scrutinized variables. Consequently, it is unequivocally deduced that the sixth treatment regimen, characterized by the administration of 99.076 ppm ZnO NPs, engenders a constructive modulatory effect upon the stature of stem elongation, stem thickness, leaf density, floral abundance, cluster profusion, and fruit yield concerning L. esculentum. This discovery corroborates the potential of ZnO NPs to prognosticate an effective augmentation in the growth and development of this pivotal crop species.

Foliar application of zinc oxide nanoparticles improves rice yield under biotic stress posed by Magnaporthe oryzae

Rice blast disease, caused by the fungus Magnaporthe oryzae, poses a significant threat to rice production worldwide. A pot experiment was conducted to test the hypothesis that foliar-applied zinc oxide nanoparticles (ZnONPs) have an inhibitory effect against blast fungus by strengthening the antioxidant defence system in rice plants. Various treatment concentrations of ZnONPs (10, 20, 30, and 40 mg/L) were applied to pathogen-inoculated plants. Foliarly applied ZnONPs lowered the infection efficiency of the sprayed conidia by 73% on the leaf segments compared to the positive control. The lesion number caused by the pathogen in the leaf tissues was significantly reduced compared to the experiment’s positive control, and the sporulation intensity was reduced compared to the positive control. Foliar-applied ZnONPs improved the functions of superoxide dismutase, phenylalanine ammonia-lyase, peroxidase, and catalase enzymes. Furthermore, the growth and yield attributes of rice plants were improved compared to the positive control. Yield and production of rice can be improved with exogenously applied ZnONPs under the rice blast infestation.

PIPES-ZnO NPs coupled catalyst for the synthesis of 2-((1H-indol-3-yl)(phenyl)methyl)-5,5-dimethylcyclohexane-1,3-diones

Synthesis, characterization and catalytic application of Zinc Oxide nanoparticles (ZnO NPs) with 1,4-Piperazinediethanesulfonic acid (PIPES) have been explored as a coupled catalyst for the condensation reaction of Dimedone (1), Benzaldehyde and (2) Indole (3) in stipulated period of time to give 2-((1H-indol-3-yl)(phenyl)methyl)-5,5-dimethylcyclohexane-1,3-dione (4) under ultrasound irradiation technique at 60 °C temperature. Pharmacological importance of targeted products along with surface properties of ZnO NPs and Bronsted acidity of PIPES together became promising advantages for this protocol to establish.

Zinc Oxide Nanoparticles Foliar Application Effectively Enhanced Zinc and Aroma Content in Rice (Oryza sativa L.) Grains

The search for an effective zinc fertilizer and its application method to effectively increase zinc content and enhance aroma in rice grains is a crucial objective. In this study, a 2-year field plot experiment was conducted to investigate the influence of ZnO NPs foliar spraying on rice quality, grain zinc and aroma content, along with exploring the physiological mechanisms underlying these effects. Our results demonstrated that the rice breakdown value and taste value of foliar spraying zinc oxide nanoparticles were improved by 31.0–41.7% and 8.2–13.0% compared with CK (control treatment involved spraying water), improving the tasting and steaming quality of rice. While Fe and Cu content in grains decreased for the application of zinc oxide nanoparticles, zinc oxide nanoparticles foliar spraying significantly increased the zinc content and accumulation of grains by 33.6–65.1% and 37.8–74.7%, respectively. Further analysis showed that the sprayed zinc oxide nanoparticles achieved effective enrichment of zinc in edible parts and increased the final bioavailability of Zn. In addition, foliar spraying of zinc oxide nanoparticles significantly increased activities of nitrate reductase and glutamine synthetase in leaves, which elevated nitrogen content in leaves and grains, and ultimately enhanced 2-acetyl-1-pyrroline (2-AP) content in grains at maturity by 6.1–21.4% compared to CK. Our findings indicated that zinc oxide nanoparticles can be practically applied as a foliar fertilizer at the gestation for quality improvement, zinc enrichment and aroma enhancement of rice grains.

Zinc oxide nanoparticles: A unique saline stress mitigator with the potential to increase future crop production

Globally, soil salinity is an abiotic stress that can threaten arable lands and crop production and consequently can negatively affect food security. Therefore, the aim of this review was to study the utilizing zinc oxide nanoparticles (ZnO-NPs) as a novel approach to mitigate salinity stress and its negative impacts on environment and crop productivity. The application of ZnO-NPs can significantly enhance plant growth, physiological and metabolic activity, and yield as well as crop quality. The antioxidant enzymes activities such as superoxide dismutase (SOD), ascorbate peroxidase (APX), and glutathione reductase (GR) are increased when ZnO-NPs are applied which can mitigate the damage caused by ROS species and make them a valuable tool for future agriculture. The sucrose synthesis which ultimately biosynthesizes lipopolysaccharides (LP), glycinebetaine (GB), and total soluble protein (TSP), and enhanced ribulose bisphosphate carboxylase-oxygenase activity, light-capturing efficiency of photosystem II, as well as the ability to transport electrons under salinity stress are increased by the application of ZnO-NPs. Therefore, ZnO-NPs can be used as a potential strategy for promoting precision agriculture. The existing yield gap under a saline environment can be minimized by promoting the application of ZnO-NPs as it serves as a cheap, environment-friendly, and efficient source of Zn. In a nutshell, being an efficient and eco-friendly nanomaterial ZnO-NPs play a key role in mitigating saline stress by alleviating NaCl toxicity and enhancing crop productivity in saline soils.

Zinc oxide nanostructures: review of eco-friendly synthesis and technological applications

The global community faces significant challenges with regard to energy and the environment. Scientists and engineers have developed innovative techniques in nanotechnology and materials science, particularly in eco-friendly synthesis of metal oxides, to address these challenges. This review discusses current trends in eco-friendly synthesis and technological applications of zinc oxide (ZnO) nanostructures beyond the biomedical field mostly presented in the literature. Green methods of zinc oxide synthesis have attracted researchers due to their low toxicity, versatility and environmentally friendly nature. Zinc oxide nanoparticles (NPs) possess unique optical and thermal characteristics, a wide band gap, high excitation binding energy and are generally recognized as safe and effective, contributing to their broad range of applications. Conventional synthesis methods have been reported to have potential toxicity effects, while green synthesis offers recognized advantages. This review also explores the mechanism of zinc oxide NP formation. Moreover, this review provides an overview of diverse applications of zinc oxide NPs, including energy devices (solar cells), cement concrete, sensors, water and environmental remediation, enhanced oil recovery, coolants and biomedicine. Further studies are recommended to explore additional properties and applications of zinc oxide NPs using the latest environmentally friendly approaches.

Enhancement of the Bioactive Compound Content and Antibacterial Activities in Curcuma Longa Using Zinc Oxide Nanoparticles.

Incorporating nanoparticles into plant cultivation has been shown to improve growth parameters and alter the bioactive component compositions of many plant species, including Curcumin longa. The aim of the current study was to investigate the effects of foliar application of zinc oxide nanoparticles on the content of bioactive compounds and their antibacterial activities against potential bacterial pathogens. To this end, C. longa leaves were treated with different doses of ZnO NPs to see how this affected their bioactive component composition. The effect of different doses of ZnO NPs on the accumulation of bisdemethoxycurcumin, demethoxycurcumin, and curcumin in ethanolic extracts of C. longa rhizomes was evaluated using high-performance liquid chromatography (HPLC). When compared to the control treatment, foliar spraying with (5 and 40 mgL-1) of ZnO NPs increased bisdemethoxycurcumin, demethoxycurcumin, and curcumin levels approximately (2.69 and 2.84)-, (2.61 and 3.22)-, and (2.90 and 3.45)-fold, respectively. We then checked whether the ethanolic extracts produced from the plantlets changed in terms of their phytochemical makeup and antibacterial properties. Furthermore, the results revealed that C. long-ZnO NPs displayed antibacterial activity against the tested S. aureus and P. aeruginosa bacterium strains, but had a few effect against E. coli. The MIC for P. aeruginosa was 100 g/mL. The time-kill studies also revealed that ZnO NPs at 4 MIC killed P. aeruginosa, Actinobacteria baumannii, and Bacillus sp. after 2 h, while S. aureus did not grow when treated with 4 × MIC of the extract for 6 h. The strongest antibacterial activity was seen in the extract from plantlets grown without nanoparticles for P. aeruginosa, whereas it was seen in the extract from plantlets grown in the presence of 5 mg/L ZnO NPs for E. coli, S. aureus, and P. aeruginosa. These findings show that ZnO NPs are powerful enhancers of bioactive compound production in C. longa, a trait that can be used to combat antibiotic resistance in pathogenic bacterial species.