Application Of Test Research Articles

MnCo2O4 Spinel Nanorods for Highly Sensitive Electrochemical Detection of Nitrite.

The rational design of nitrite sensors has attracted significant research interest due to their widespread use and the associated risks of methemoglobinemia and carcinogenicity. The undisclosed nitrite-sensing performance of the spinel cobaltite MnCo2O4 (MCO) prepared by an oxalate-assisted coprecipitation method is reported in this study. Spectroscopy and microscopy investigations revealed the formation of uniform MCO nanorods with a high aspect ratio. The electrocatalytic nitrite oxidation at the MCO-coated glassy carbon electrode (MCO/GCE) indicated the promising performance of the synthesized material for nitrite sensing. MCO/GCE detects nitrite in a concentration range of 5 μM to 3 mM and has a limit of detection of 0.95 μM with a higher sensitivity of 857 μA mM-1 cm-2 in a response time of 4 s. In MCO, the mixed-valence states of Co2+/Co3+ confer a high electrical conductivity, and higher valent redox couples of Mn and Co impart remarkable electrocatalytic activity toward nitrite oxidation. MCO spinel undergoes facile and ultrafast faradaic reactions to mediate nitrite oxidation. Additionally, the mesopores of MCO nanorods facilitate the rapid diffusion of electrolyte and nitrite ions. Employing the electrode in sensing nitrite in milk, lake, and tap water samples further validates its potential application in real-life testing. MCO spinel nanorods showcase promising scope for utilization in the electrochemical sensing of nitrite and inspire further exploration of transition-metal oxide-based mixed-spinel materials.

Undrained cavity expansion-contraction analysis in CASM and its application for pressuremeter tests

Many geotechnical scenarios involve cavity unloading from a loaded state, particularly in pressuremeter tests, and the unloading data of pressuremeter tests has exceptional attraction as it is less disturbed by the insertion process. However, the analyses for continuous cavity loading and unloading (i.e., cavity initially experiences expansion and then contracts) in critical state soils are rarely studied. To this end, a novel semi-analytical solution based on the unified state parameter model for clay and sand (CASM) is proposed for the whole expansion-contraction of spherical and cylindrical cavities under undrained condition. The problem assumes that the cavity is unloaded after a monotonic loading stage, leading to plastic regions during both loading and unloading periods. The cavity response for the whole expansion-contraction process is investigated, with the total pressure and stress paths at the cavity wall presented and validated against numerical simulation. The developed solution is successfully implemented to interpret both loading and unloading data of pressuremeter tests. The undrained shear strength, in-situ effective horizontal stress and initial overconsolidation ratio are back analyzed by using a curve fitting method based on the proposed solution.

Development and Validation of an Android-based Trail Making Test Application (TMT App) for Cognitive Evaluation in Healthy and Depressed Individuals

Background: The Trail Making Test (TMT) is a famous neuropsychological test that is frequently used. The structure of a paper-and-pencil-based TMT is highly likely to be adapted to a mobile app. Objective: The current study aimed to develop and validate an Android-based tablet version of TMT. Methods: The application (TMT App) was developed using an Android-based platform. Healthy and depressed individuals ( n = 133) were assessed on both the TMT versions (paper-based version and app-based version) in a random cross-over design. The device’s usability was ascertained using the system usability scale (SUS) in a subset of individuals ( n = 65). Results: There was a significant positive correlation between the individual processing times for the paper-based TMT-A and the app-based TMT-A in both healthy and depression groups [r(63) = 0.55, p < .001; and r(66) = 0.77, p < .001, respectively]. The individual processing times of the paper-based TMT-B and the app-based TMT-B also showed a significant positive correlation in both healthy control and depression groups [r(63) = 0.67, p < .001; and r(66) = 0.89, p < .001, respectively]. There was a positive correlation of age with TMT-A and TMT-B for either version. Both groups had similar positive responses to the usability of the TMT App. Conclusion: The preliminary validation results for the TMT App suggest that it is significantly correlated with existing paper-and-pencil methods, and that it is user friendly.

Application of language tests in university education. Evaluation of a formative process.

La aplicación de pruebas lingüísticas en contextos reales es una competencia fundamental que los estudiantes de los grados en Logopedia y en Educación Primaria deben aprender. El objetivo de esta investigación consiste en comprender y evaluar el proyecto formativo universitario llevado a cabo para que los estudiantes aprendan a aplicar pruebas lingüísticas. El proyecto se divide en tres fases: los estudiantes graban la aplicación de las pruebas lingüísticas, los profesores enseñan el proceso de evaluación, y otros estudiantes analizan y corrigen las pruebas grabadas. Un grupo de 200 participantes completa un cuestionario que incluye preguntas sobre las dificultades y desafíos encontrados y la idoneidad de la práctica. Se valora por separado la enseñanza de pruebas estandarizadas/baterías y la enseñanza de protocolos/registros. Los resultados sugieren que las actividades propuestas son apropiadas. Aunque no se observan diferencias significativas entre los grupos en cuatro variables, surgen disparidades en la comprensión de los vídeos, la toma de notas en las hojas de registro, la elaboración de conclusiones y la redacción de informes. Lo que significa que esta investigación es útil para aquellos docentes que estén interesados en llevar a cabo proyectos formativos con aplicación práctica en el aula y poder así generalizar el proceso formativo a otras universidades.

Applicability of the Pancreatic Exocrine Insufficiency Test (PEI-TEST) in Pediatric Patients.

In mild cases, it is difficult to diagnose pancreatic exocrine insufficiency (PEI). There is no gold standard method for the diagnosis of PEI. A reliable method is needed for preliminary diagnosis of PEI. The PEI-TEST was applied to the patients with nonspecific gastrointestinal complaints. Serum amylase, lipase, serum trypsinogen, and fecal elastase 1 (FE-1) were analyzed from each patient. According to the PEI-TEST, PEI was present in 42 (47.7%) and PEI was not present in 46 (52.3%) patients. No significant difference was observed between the 2 groups with regard to age, gender and amylase, lipase, serum trypsinogen, and FE-1. When an FE-1 value of <200 µg/dL was considered as indicating PEI, the sensitivity and specificity of the test were found to be 47.4% and 52.2%, respectively. Although it is promising that PEI-TEST is a validated test in our country and suitable for our society, it is not suitable for pediatric patients.

The Utilization of Sociometric Tests in Identifying School Integration Challenges Among Students with Immigrant Parents.

The purpose of this study is to investigate how sociometric relationships are configured within the primary and secondary school group that have immigrant parents. The analysis of the specialized literature was carried out based on clear selection criteria, both chronologically and of the variables. Statistical analyses, including correlations and frequency calculations, were conducted for the research variables. The study also clarifies certain procedural aspects in the application of sociometric tests. Our study included four school groups, two with 4th-grade students and two with pre-adolescent high school students. Parental emigration is a phenomenon that can have significant negative effects on the school integration of children who are left behind. They may experience emotional stress and anxiety that can affect their school performance. Some may also face financial difficulties, resulting in material shortages necessary to participate in school activities. The overload of tasks and household responsibilities can lead to their stigmatization in school groups. Our study examined three dimensions of school integration: partnership in work activities, participation in leisure time activities, and leadership within the group. The results of the research indicate a poor sociometric positioning for the children of emigrants, who are often rejected by the group for work activities, ignored in terms of spending free time, and overlooked for leadership roles within the group. Even if there are situations where some of them gain followers, they are also among the students rejected by the group. Most of them are ignored or rejected by the more socially prominent students in the class,

Tools for telehealth: A correlational analysis of app-based hearing testing.

Telehealth evaluation of hearing is rapidly evolving; however, the lack of consensus on the most accurate remote hearing test application has made hearing evaluation complicated. The objective of this study was to evaluate the correlation between the pure tone audiometry results obtained from app-based hearing testing programs and a traditional audiogram. A prospective within-subject and between-subject study design was used to correlate audiogram results between app-based hearing programs and a traditional audiogram. All participants completed a traditional audiogram, 1 commercial app-based test (ShoeBox), 2 consumer app-based tests (EarTrumpet and Hearing Test and Ear Age Test [HTEAT]), and a Hearing Handicap Inventory screening version (HHI-S). Testing was conducted in an acoustically controlled environment (traditional) and a quiet room (app-based hearing tests). A total of 39 participants were enrolled in the study (21 with normal hearing and 18 with hearing loss). In patients with normal hearing, only the commercial hearing testing app (ShoeBox) had a statistically significant pure tone average correlation in both ears with traditional audiometry (Right ear-r = 0.7, p = .005, Left ear-r = 0.66, p = .001). Both consumer and commercial apps had statistically significant correlations with both ears in patients with hearing loss (ranging from r = 0.62 to r = 0.9). Regarding accuracy within 10 dB of the pure tone average of the traditional audiogram of all tested ears, the commercial app-based test was accurate in 94% for all ears (normal and hearing loss), while consumer app-based tests were between 14% and 36% for all ears. The HHI-S indicated no hearing impairment in 95% of those with normal hearing and indicated hearing impairment in 89% of those with hearing loss. Commercial-grade app-based pure tone audiometry demonstrates overall strong correlation and accuracy with traditional audiometry. The HHI-S assessment remains a valid and useful tool to predict normal hearing and hearing impairment. 2.

Customized carbon dots as a fluorescent sensor for detecting paraquat through enhanced excited state binding energy

A novel fluorescent sensor utilizing customized carbon dots (CDs) has been developed for the rapid and efficient detection of the herbicide paraquat. The 4,4′-diamino-2,2′-stilbenedisulfonic acid (DSD acid) functionalized CDs (DSD-CDs) were synthesized by a hydrothermal method using citric acid and L-cysteine as precursors, followed by amidation with DSD acid. The resultant DSD-CDs exhibited bright blue fluorescence upon UV excitation. The fluorescence of the functionalized carbon dots was effectively quenched by paraquat, following a static quenching mechanism as confirmed by fluorescence lifetime measurements. Interestingly, the mechanism study revealed that the excited-state of the DSD-CDs could interact effectively with paraquat, resulting in stronger fluorescence quenching compared to the ground-state. The sensor showed high sensitivity, selectivity, and stability, with a wide linear range of 0.01 ∼ 2 mg/L (R2 = 0.997) and a low detection limit of 0.003 mg/L. The developed fluorescent sensor demonstrated exceptional potential for paraquat detection in actual samples, encompassing blood, urine, soybean, and river water, with recovery rates exceeding 83 % and relative standard deviations (RSD) below 10 %. This underscores its practical utility in environmental protection and clinical testing applications.

A Portable Readout System for Biomarker Detection with Aptamer-Modified CMOS ISFET Array

Biosensors based on ion-sensitive field effect transistors (ISFETs) combined with aptamers offer a promising and convenient solution for point-of-care testing applications due to the ability for fast and label-free detection of a wide range of biomarkers. Mobile and easy-to-use readout devices for the ISFET aptasensors would contribute to further development of the field. In this paper, the development of a portable PC-controlled device for detecting aptamer-target interactions using ISFETs is described. The device assembly allows selective modification of individual ISFETs with different oligonucleotides. Ta2O5-gated ISFET structures were optimized to minimize trapped charge and capacitive attenuation. Integrated CMOS readout circuits with linear transfer function were used to minimize the distortion of the original ISFET signal. An external analog signal digitizer with constant voltage and superimposed high-frequency sine wave reference voltage capabilities was designed to increase sensitivity when reading ISFET signals. The device performance was demonstrated with the aptamer-driven detection of troponin I in both reference voltage setting modes. The sine wave reference voltage measurement method reduced the level of drift over time and enabled a lowering of the minimum detectable analyte concentration. In this mode (constant voltage 2.4 V and 10 kHz 0.1Vp-p), the device allowed the detection of troponin I with a limit of detection of 3.27 ng/mL. Discrimination of acute myocardial infarction was demonstrated with the developed device. The ISFET device provides a platform for the multiplexed detection of different biomarkers in point-of-care testing.

Genetic Causes of Qualitative Sperm Defects: A Narrative Review of Clinical Evidence

Several genes are implicated in spermatogenesis and fertility regulation, and these genes are presently being analysed in clinical practice due to their involvement in male factor infertility (MFI). However, there are still few genetic analyses that are currently recommended for use in clinical practice. In this manuscript, we reviewed the genetic causes of qualitative sperm defects. We distinguished between alterations causing reduced sperm motility (asthenozoospermia) and alterations causing changes in the typical morphology of sperm (teratozoospermia). In detail, the genetic causes of reduced sperm motility may be found in the alteration of genes associated with sperm mitochondrial DNA, mitochondrial proteins, ion transport and channels, and flagellar proteins. On the other hand, the genetic causes of changes in typical sperm morphology are related to conditions with a strong genetic basis, such as macrozoospermia, globozoospermia, and acephalic spermatozoa syndrome. We tried to distinguish alterations approved for routine clinical application from those still unsupported by adequate clinical studies. The most important aspect of the study was related to the correct identification of subjects to be tested and the correct application of genetic tests based on clear clinical data. The correct application of available genetic tests in a scenario where reduced sperm motility and changes in sperm morphology have been observed enables the delivery of a defined diagnosis and plays an important role in clinical decision-making. Finally, clarifying the genetic causes of MFI might, in future, contribute to reducing the proportion of so-called idiopathic MFI, which might indeed be defined as a subtype of MFI whose cause has not yet been revealed.

Decoding pharmacogenomic test interpretation and application to patient care

AbstractPharmacogenomics is a growing area of medicine, and pharmacists across clinical practice settings have the opportunity to individualize medication selection and dosing using genetic data. However, many practicing pharmacists may feel ill‐equipped to interpret pharmacogenomic test results because of insufficient education and training. Evidence‐based, updated, and freely available resources such as the Clinical Pharmacogenetics Implementation Consortium guidelines can help pharmacists interpret and apply pharmacogenomic test results to patient care. Although gaps for the application of pharmacogenomic information exist, this commentary aims to demystify the interpretation of pharmacogenomic test results and empower pharmacists to apply genetic data alongside other clinical variables to optimize medication‐related outcomes for their patients. An “ABCD” framework is proposed to guide pharmacists through the steps: (1) Actionability—Are the gene(s) clinically relevant for the patient? (2) Be Mindful of Limitations—What are the caveats with pharmacogenomic test results and reports? (3) Clinical Practice Guidelines—How do you use pharmacogenomic test results to guide clinical decision‐making? and (4) Document and Discuss—How do you educate the patient about their pharmacogenomic test results and document the results for future use? Key concepts are illustrated using a psychiatric patient case example.

Scaling analysis of nuclear power plant (APR1400) utilizing ATLAS integral effect test result of A5.1 (Counterpart test for LSTF 1% cold-leg break LOCA)

ATLAS is the thermal hydraulic integral effect test loop that can simulate the same pressure and temperature condition with nuclear power plant. It was designed with a height ratio of 1/2 and a volume ratio of 1/288 with reference to APR1400. The counterpart test against LSTF which was named as A5.1 test was performed within the OECD/NEA ATLAS international joint research project to compare major thermal hydraulic phenomena between two test facilities. The main scenario for the A5.1 test is the LSTF SB-CL-32 test. The SB-CL-32 test is a 1% horizontal small break loss of coolant accident (SBLOCA) at the cold leg with cold leg injection of the emergency core cooling system (ECCS) and accident management (AM) action.From the counterpart test results, the significant differences were found in the viewpoint of the thermal–hydraulic phenomena. It was concluded that the different result were caused by the different design characteristic of each test facility because they referred the different nuclear power plant. Thus, the applicability of the ATLAS test results to its reference nuclear power plant was evaluated by utilizing ATLAS A5.1 test results which was the counterpart test with the LSTF.The transient scenario and initial and boundary conditions of the A5.1 test were adopted to APR1400 after applying scaling ratios of ATLAS. The MARS-KS code was utilized to analyzed thermal hydraulic behaviors of APR1400 and the analysis results were compared with the scaled A5.1 test result. From the result of comparison, it was concluded that the ATLAS test results predicted the thermal hydraulic behavior of the nuclear power plant well. Thus, it was confirmed that ATLAS is a well-designed integral effect test facility which is designed with an appropriate scaling method.

Sensors constructed from carbon nanomaterials and their applications

Diabetes is a growing disease, with patient morbidity and mortality increasing every year. Since diabetes is associated with short- and long-term health problems, it has become extremely important to detect and effectively treat diabetes. Many research institutes and healthcare organizations are leaning towards the use of cost-effective biosensors to monitor the body's blood glucose standards and provide accurate health diagnosis. Biosensors help in accurate diabetes diagnosis and monitoring for effective treatment and management. Based on the recent advances in nanotechnology, a variety of glucose biosensors made of carbon nanomaterials have been developed, and such sensors are a key factor for diabetes diagnosis and treatment, with better performance and sensitivity for better detection of the disease and tracking of the response to treatment. Thus, this research illustrates the significant advances in nanotechnology-based biosensors for medical applications and the potential of carbon nanomaterials for good applications in medical testing.

Research status of colloidal gold detection technology in medicine

Research status of colloidal gold detection technology in medicine

Coupled slow strain rate and acoustic emission tests for gaseous hydrogen embrittlement assessment of API X65 pipeline steel

Hydrogen embrittlement (HE) sensitivity of API X65 pipeline steel was evaluated in this study through the simultaneous application of slow strain rate tests (SSRT) and acoustic emission (AE) monitoring on hollow dog-bone samples. Exposure to hydrogen gas decreased the elongation to fracture percentage with a significant rise in embrittlement index for the higher pressure of 10 MPa. SEM images of the fracture surfaces demonstrated that the fracture mechanism changed from ductile to ductile–brittle under 1 MPa pressure, and to predominantly brittle with quasi-cleavage characteristic when testing under 10 MPa hydrogen gas. Classification of the AE events based on waveform analysis revealed a mainly shear mode fracture for 10 MPa nitrogen sample, while a dominant tensile mode fracture was identified for the hydrogen charged samples.

Features of diagnostics and treatments of the patient with type 2 diabetes mellitus and chronic ischtmia with threating lower limb loss

The article presents the result of our own observation of the patient with a poor control of type 2 diabetes mellitus (DM) for a long period, complicated by obliterating atherosclerosis of the arteries of the lower extremities, Menckeberg’s sclerosis and chronic ischemia threatening loss of the lower extremity (CLLI). A feature of the clinical manifestation are complications associated with potentially regional (angiosomal) ischemia of the foot, as well as variant anatomy, represented by hypoplasia of the vascular lower leg in the patient. The clinical consequences of vascular calcification due to long-term decompensation of carbohydrate metabolism and the development of diabetic distal polyneuropathy (DDP) led to falsely high values of the cuff test in the patient. Disadvantages of non-invasive methods for diagnosing limb ischemia and advantages of the complex application of tests for diseases of the arteries of the lower extremities are discussed. Using WIFI classification according to the degree of ulceration, the degree of ischemia, and the degree of infection on the foot (Wound, Ischemia and Foot Infection), the tactics of managing the patient are presented. The important role of ultrasonic duplex scanning (USDS) in the visualization of the arteries of the legs and feet in patients with DM is substantiated. The importance of a multidisciplinary approach in the management of a comorbid patient with type 2 diabetes and CLTI is emphasized.

Bending Strength Evaluation of Japanese Wooden Cultural Heritage Building Columns Based on a Non-Destructive Testing Method

ABSTRACT Column failure assessment is essential for seismic diagnosis of Japanese wooden cultural heritage buildings. Achieving accurate column failure assessment depends on accurately estimating the bending Young’s modulus (E b ) and characteristic bending strength (F b ) of columns. This study extends the application of a non-destructive testing (NDT) method to estimate E b and F b of Japanese cedar columns generally used in wooden cultural heritage buildings. For this purpose, a correlation between the dynamic bending Young’s modulus (E p ) and E b of Japanese cedar columns is established and the variations in the E p –E b relationship across different wood provenances and species are clarified. Using the proposed method, a statistical analysis of the estimated E b of Japanese wooden cultural heritage buildings is provided. Furthermore, estimated E b is used to determine F b of the columns using the mechanical grade classification system. The results show that most Japanese cypress and Japanese cedar columns feature higher F b than those used in conventional seismic diagnosis. Conversely, two-thirds of the Japanese red pine columns feature a lower F b than that used in conventional seismic diagnosis. The proposed F b evaluation method offers an NDT method that can be widely applied to Japanese wooden cultural heritage buildings to assess column failure more accurately.

Applicability of Single-Borehole Dilution Tests in Aquifers with Vertical Flow

A set of experimental field single-borehole dilution tests were completed in the Motril–Salobreña detrital aquifer (Spain) in a sector with coarse material in four different moments under variable hydrological conditions. The comparative study of the tracer washing, and the temperature profile patterns for the tests carried out in two wells located hundreds of m from each other, revealed the presence of ascending vertical flows in one of the wells (not detected by other means) that compromises the reliability of the tracer test. The values of both the apparent horizontal velocity and hydraulic conductivity obtained in the affected well were less than half of those estimated in the well not affected by the upward vertical flows. The repetition of the test eight times during different seasons showed that the hydraulic conductivity calculated from the apparent horizontal velocity can vary; therefore, to approximate to a representative hydraulic conductivity value, using this method is recommended to carry out tests under different hydrological conditions and average the results. The difference generated by the changes in conditions for the specific setting of the study area was 25%. Taking this into account, it was considered that an approximation to the more representative value would be an average under variable hydrological conditions, resulting in a horizontal velocity of 6.7 m/d and hydraulic conductivity of 337 m/d. This information is critical for the management of the aquifer as it has strategic resources against droughts that are becoming more frequent in the Mediterranean area.

Temporal Variations in Land Surface Temperature within an Urban Ecosystem: A Comprehensive Assessment of Land Use and Land Cover Change in Kharkiv, Ukraine

Remote sensing technologies are critical for analyzing the escalating impacts of global climate change and increasing urbanization, providing vital insights into land surface temperature (LST), land use and cover (LULC) changes, and the identification of urban heat island (UHI) and surface urban heat island (SUHI) phenomena. This research focuses on the nexus between LULC alterations and variations in LST and air temperature (Tair), with a specific emphasis on the intensified SUHI effect in Kharkiv, Ukraine. Employing an integrated approach, this study analyzes time-series data from Landsat and MODIS satellites, alongside Tair climate records, utilizing machine learning techniques and linear regression analysis. Key findings indicate a statistically significant upward trend in Tair and LST during the summer months from 1984 to 2023, with a notable positive correlation between Tair and LST across both datasets. MODIS data exhibit a stronger correlation (R2 = 0.879) compared to Landsat (R2 = 0.663). The application of a supervised classification through Random Forest algorithms and vegetation indices on LULC data reveals significant alterations: a 70.3% increase in urban land and a decrement in vegetative cover comprising a 15.5% reduction in dense vegetation and a 62.9% decrease in sparse vegetation. Change detection analysis elucidates a 24.6% conversion of sparse vegetation into urban land, underscoring a pronounced trajectory towards urbanization. Temporal and seasonal LST variations across different LULC classes were analyzed using kernel density estimation (KDE) and boxplot analysis. Urban areas and sparse vegetation had the smallest average LST fluctuations, at 2.09 °C and 2.16 °C, respectively, but recorded the most extreme LST values. Water and dense vegetation classes exhibited slightly larger fluctuations of 2.30 °C and 2.24 °C, with the bare land class showing the highest fluctuation 2.46 °C, but fewer extremes. Quantitative analysis with the application of Kolmogorov-Smirnov tests across various LULC classes substantiated the normality of LST distributions p &gt; 0.05 for both monthly and annual datasets. Conversely, the Shapiro-Wilk test validated the normal distribution hypothesis exclusively for monthly data, indicating deviations from normality in the annual data. Thresholded LST classifies urban and bare lands as the warmest classes at 39.51 °C and 38.20 °C, respectively, and classifies water at 35.96 °C, dense vegetation at 35.52 °C, and sparse vegetation 37.71 °C as the coldest, which is a trend that is consistent annually and monthly. The analysis of SUHI effects demonstrates an increasing trend in UHI intensity, with statistical trends indicating a growth in average SUHI values over time. This comprehensive study underscores the critical role of remote sensing in understanding and addressing the impacts of climate change and urbanization on local and global climates, emphasizing the need for sustainable urban planning and green infrastructure to mitigate UHI effects.

Cost-effectiveness of genetic testing of endocrine tumor patients using a comprehensive hereditary cancer gene panel.

Heterogenous clinical manifestations, overlapping phenotypes and complex genetic backgrounds are common in patients with endocrine tumors. There are no comprehensive recommendations for genetic testing and counselling of these patients compared to other hereditary cancer syndromes. The application of multigene panel testing is common in clinical genetic laboratories, but their performance for patients with endocrine tumors has not been assessed. As a national reference center, we prospectively tested the diagnostic utility and cost-efficiency of a multigene panel covering 113 genes representing genetic susceptibility for solid tumors. 1279 patients (including 96 cases with endocrine tumors) were evaluated between October 2021 and December 2022 who were suspected to have hereditary tumor syndromes. The analytical performance of the hereditary cancer panel was suitable for diagnostic testing. Clinical diagnosis was confirmed in 24% (23/96); incidental findings in genes not associated with the patient's phenotype were identified in 5% (5/96). A further 7% of pathogenic/likely pathogenic variants were detected in genes with potential genetic susceptibility roles but currently no clear clinical consequence. Cost-benefit analysis showed that the application of a more comprehensive gene panel in a diagnostic laboratory yielded a shorter turnaround time and provided additional genetic results with the same cost and workload. Using comprehensive multigene panel results in faster turnaround time and cost-efficiently identifies genetic alterations in hereditary endocrine tumor syndromes. Incidentally identified variants in patients with poor prognoses may serve as a potential therapeutic target in tumors where therapeutic possibilities are limited.