Nitrogen Application Research Articles

Optimizing Irrigation and Nitrogen Application to Enhance Millet Yield, Improve Water and Nitrogen Use Efficiency and Reduce Inorganic Nitrogen Accumulation in Northeast China

Enhancing irrigation and nitrogen fertilizer application has become a vital strategy for ensuring food security in the face of population growth and resource scarcity. A 2-year experiment was conducted to determine to investigate the effects of different irrigation lower limits and nitrogen fertilizer application amounts on millet growth, yield, water use efficiency (WUE), N utilization, and inorganic nitrogen accumulation in the soil in 2021 and 2022. The experiment was designed with four irrigation lower limits, corresponding to 50%, 60%, 70%, and 80% of the field capacity (FC), referred to as I50, I60, I70, and I80. Four nitrogen fertilizer application were also included: 0, 50, 100, and 150 kg·hm−2 (designated as F00, F50, F100, and F150), resulting in a total of 16 treatments. Binary quadratic regression equations were established to optimize the irrigation and nitrogen application. The results demonstrated that the plant height, stem diameter, leaf area index, aboveground biomass, yield, spike diameter, spike length, spike weight, WUE, and nitrogen agronomic efficiency for millet initially increased before subsequently decreasing as the irrigation lower limit and nitrogen fertilizer application increased. Their maximum values were observed in the I70F100. However, the nitrogen partial factor productivity (PFPN) exhibited a gradual decline with increasing nitrogen application, reaching its peak at F50. Additionally, PFPN displayed a pattern of initial increase followed by a decrease with rising irrigation lower limits. The accumulation of NO3−-N and NH4+-N in the 0~60 cm soil layer increased with the increase of nitrogen fertilizer application in both years, while they tended to decrease as the irrigation lower limit increased. An optimal irrigation lower limit of 64% FC to 74% FC and nitrogen fertilizer application of 80 to 100 kg ha−1 was recommended for millet based on the regression equation. The findings of this study offer a theoretical foundation and technical guidance for developing a drip irrigation and fertilizer application for millet cultivation in Northeast China.

Synergistic Effects of Irrigation and Nitrogen Fertilisation on Maize Photosynthetic Performance and Yield of Rainfed Systems in Drought‐Prone Environments

ABSTRACTMaize, a cereal crop of global significance, encounters cultivation challenges in the subtropical regions of Guangxi, mainly due to variable rainfall and low soil fertility, exacerbating the effects of drought. This study evaluated the effects of irrigation and nitrogen fertilisation on overcoming these challenges and improving maize growth and yield. Between 2020 and 2021, a split‐plot experiment was conducted. The main plots were assigned to two irrigation treatments: irrigated and rainfed. Within each main plot, subplots were treated with different nitrogen levels (0, 150, 200, 250 and 300 kg ha−1). The results showed that nitrogen levels and water regime significantly impacted several key factors, including the net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), intercellular carbon dioxide concentration (Ci), photosynthetically active radiation (PAR), carbon‐metabolising enzymes and total carbon (TC) content accumulation. Under drought‐like rainfed conditions, the application of nitrogen, RN300 (rainfed application nitrogen 300 kg ha−1), IN250 (irrigated application nitrogen 250 kg ha−1) significantly enhanced the Pn (10.0%), Tr (3.17%), Ci (3.41%) and Gs (2.6%). Additionally, PAR was significantly influenced by the water regime and nitrogen levels. Under IN250, the capture ratio (Ca) increased (2.36%), while the penetration ratio (Pe) and reflectance ratio (Re) decreased by 13.12% and 46.36%, respectively, compared to RN300. The levels of carbon metabolism enzymes (sucrose phosphate synthase and phosphoenolpyruvate carboxylase) and the TC content were higher under RN300 compared to IN250; however, these differences were not statistically significant. Path analysis revealed that thousand kernel weight had the most significant impact on yield under both water regimes. The effect was stronger under irrigated conditions, with a path coefficient of 0.647, compared to 0.459 under rainfed conditions. Correlation analysis indicated that plant height (0.938), stem diameter (0.906), ear diameter (0.928) and ear length (0.803) were positively correlated with nitrogen levels. In conclusion, maize under IN250 exhibited superior photosynthetic performance and carbon accumulation. This suggests that balanced irrigation and nitrogen management can effectively mitigate the adverse impacts of drought on maize, optimising growth and yield sustainably.

Maize biomass yield variations due to diverse nitrogen applications using Aquacrop

AbstractIn the quest to increase crop yields and production intensity to meet the demands of a growing global population, the responsible use of nitrogen (N) applications is paramount. Excessive application of these fertilizers results in economic losses, environmental pollution and reduced N‐use efficiency. To address this challenge, precision agriculture techniques offer promising solutions. This study explored the relationships among applied N, maize crop parameters and the nitrogen nutrition index (NNI) to optimize N application usage and crop performance. The AquaCrop model was used to simulate water productivity and plant parameters accurately. The research establishes equations between the NNI and maize parameters, enabling the use of AquaCrop to simulate crop responses to varying N levels. The results demonstrate that AquaCrop effectively simulates more than 95% of the biomass changes. The findings suggest that applying N based on presented equations optimized the use of N applications, thereby mitigating economic losses and environmental impacts.

Combined Application of Nitrogen and Sulfur Improves Growth, Oil, and Bio-Diesel Production from Soybean

Combined Application of Nitrogen and Sulfur Improves Growth, Oil, and Bio-Diesel Production from Soybean

Reinforcing Nitrogen Nutrition Through Partial Substitution with Organic Nitrogen Enhances the Properties of Natural Rubber

The partial substitution of chemical fertilizer with organic fertilizer is a crucial practice for enhancing crop production and quality, although its impact on natural rubber has rarely been explored. In this study, a two-year field experiment was conducted to investigate the impact of different nitrogen application rates and varying proportions of organic nitrogen substitution on dry rubber yield, nitrogen nutrition, and natural rubber properties. Regarding nitrogen application, the control treatment received no nitrogen amendment, while the low-nitrogen treatment was amended with 138 g·tree−1·year−1 of nitrogen. The medium-nitrogen treatment received 276 g·tree−1·year−1 of nitrogen, and the high-nitrogen treatment received 552 g·tree−1·year−1 of nitrogen. In addition, the low-organic-nitrogen substitution treatment and medium-organic-nitrogen substitution treatment were amended with 276 g·tree−1·year−1 of nitrogen each. The results demonstrated that the 50% organic nitrogen substitution treatment resulted in the highest dry rubber yield across all sampling periods, ranging from 46.43 to 94.65 g·tree−1. Additionally, this treatment exhibited superior soil total nitrogen (1067.69 mg·kg−1), available nitrogen (84.06 mg·kg−1), and nitrogen content in roots (1.08%), leaves (3.25%), fresh rubber latex (0.27%), and raw natural rubber (0.44%) compared with other treatments. In terms of the physical properties of natural rubber, the 50% organic nitrogen substitution treatment resulted in advantages in the weight-average molecular weight (1.57 × 106 g·mol−1), number-average molecular weight (0.36 × 106 g·mol−1), plasticity retention index (97.35%), Wallace plasticity (40.25), and Mooney viscosity (81.40). For mechanical properties, natural rubber from the substitution treatment exhibited higher tensile strength (19.84 MPa), greater elongation at break (834.75%), and increased tear strength (31.07 N·mm−1). Overall, the substitution of 50% chemical nitrogen fertilizer with organic nitrogen fertilizer improved nitrogen nutrition in rubber trees by introducing organic nitrogen input, resulting in remarkable enhancements in natural rubber properties. Therefore, the incorporation of organic fertilizer as a substitution for 50% of chemical fertilizer is demonstrated as an effective strategy for improving both the yield and properties of natural rubber.

Nitrogen use efficiency for seed and oil yield in some Moroccan rapeseed (Brassica napus L.) varieties under contrasting nitrogen supply

In the actual context of climate change and increasing oil and nitrogen prices, oil crops varieties with high nitrogen use efficiency (NUE) will be highly appreciated. As no studies on NUE in spring rapeseed have been carried out in Mediterranean area, including Morocco, this work aimed to assess responses of six rapeseed varieties to six nitrogen levels (0, 30, 60, 90, 120, and 150 Kg N/ha) and their NUE for grain and oil yields. The experiment was conducted according to a split-plot design with three replications, during two cropping seasons. Results showed that increasing nitrogen rate from 0 to 120 kg N/ha led to a significant increase in seed, oil and dry matter yields up to 85.3, 72.35 and 97.9%, respectively. NUE, NUpE (nitrogen uptake efficiency) and NUtE (nitrogen utilization efficiency) decreased by increasing nitrogen application. Regarding oil content, it was negatively affected by nitrogen fertilization, declining from 40.78% for control to 37.77% for 150 kg N/ha. Likewise, nitrogen use and nitrogen utilization efficiency for oil production (ONUE & ONUtE) were negatively affected by nitrogen application. The genotypic variation was statistically significant for all investigated traits. The variety hybrid ‘Trapper’ was the most interesting for ONUE, NUE, seed yield and dry matter. However, for oil yield it was comparable to ‘Moufida’ and ‘Alia’ which showed the highest values for harvest index, branching, pods number per plant, oil content and ONUE. These varieties grown under 120 kg N/ha treatment can be recommended for Morocco.

Preferential Carbon Allocation Into Vegetative Ramets and Belowground Organs During the Seed-Filling Stage Limits Seed Set in Leymus chinensis.

Clonal perennial grasses are the dominant species in almost all natural grasslands, however their seed production is typically low. The reasons why seed set is so low remains unclear. We studied a rhizomatous grass (Leymus chinensis) using 13C tracing the different photosynthetic organs to investigate carbon fixation and allocation during the seed-filling stage. We found that the vegetative ramet leaves are the largest (81%) source for total plant fixed carbon, whereas almost all carbon is allocated to vegetative reproduction. The spike is the largest (54%) carbon source for the seeds. However, the spike produced carbon only allocated 37% to the seeds, with the majority allocated to vegetative reproduction. This preferential carbon allocation to vegetative reproduction limits sexual reproduction. Nitrogen application significantly increased assimilated carbon. However, nearly all increased carbon accumulated in the vegetative reproduction rather than in the seeds. Only the carbon produced by the spike increased its allocation to the seeds by 13%. Taken together, we conclude that the predominance of vegetative reproduction, combined with self-incompatibility, results in low ovule fertilization and very weak seed sink strength for carbon competition, suggests that the weak seed sink strength is the key reason causing low seed set in L. chinensis.

Cenchrus spinifex Invasion Alters Soil Nitrogen Dynamics and Competition

Invasive plants often alter biological soil conditions to increase their own competitiveness. Through indoor simulated nitrogen deposition culture experiments, we investigated the differences in growth indicators and nutrient content levels between the invasive plant Cenchrus spinifex Cav. and the native symbiotic plant Agropyron cristatum (L.) Gaertn. under diverse nitrogen application modes and planting-competition ratios. Furthermore, we examined the alterations in key microbial communities involved in soil nitrogen cycling of C. spinifex. The results indicated that the invasion of C. spinifex could inhibit the growth of native plants, and in fact altered the accumulation and transformation processes related to soil nitrogen, resulting in reduced rates of soil nitrogen transformation. The overarching aim of this research was to construct a theoretical foundation for the scientific comprehension of the invasion mechanisms of C. spinifex, in order to better prevent the further spread of this invasive plant and mitigate its pernicious impact on the current environment.

Study on Optimal Nitrogen Application for Different Oat Varieties in Dryland Regions of the Loess Plateau

The present study endeavored to tackle the challenges posed by limited diversity in oat varieties and suboptimal nitrogen fertilizer utilization in the arid landscapes of the Loess Plateau. We selected three oat varieties, including early-maturing oats (E), medium-maturing oats (M), and late-maturing oats (L). In 2022, four nitrogen applications were set up as CK (0 kg N ha−1), N1 (60 kg N ha−1), N2 (90 kg N ha−1), and N3 (120 kg N ha−1). We introduced two additional nitrogen applications, N4 (180 kg N ha−1) and N5 (240 kg N ha−1), in 2023. The two-year study results demonstrated a significant increase in oat yield due to nitrogen application (p < 0.05). The highest grain yield was observed for E oats at 2216.63 kg·ha−1 under the N3 treatment, while M and L oats had the highest grain yields at 2505.43 kg·ha−1 and 2946.30 kg·ha−1 under N4, respectively. The protein content of L oats reached a peak of 14.15% under N4, and the order of protein contents in oat protein components was globulin > gliadin> glutenin > albumin. The β-glucan content of L oats reached a peak of 4.92% under N3. The nitrogen fertilizer utilization efficiency (NFUE) of the three oats was highest under N2. L oats exhibited enhanced NFUE owing to an elevated pre-flowering nitrogen translocation amount (PrNTA), with a 42.94% and 29.51% increase relative to E and M oats, respectively. The pre-flowering nitrogen translocation contribution (PrNTC) in oats surpassed the post-flowering nitrogen accumulation contribution (PoNAC). Therefore, nitrogen application positively impacted oat growth, yet excessive application had an inhibitory effect. There is a significant positive correlation among oat yield, quality, nitrogen accumulation, and utilization efficiency. In summary, oat crops exhibited optimal performance in terms of yield, quality, and nitrogen use efficiency when nitrogen application rates ranged between 90 and 180 kg·ha−1. Late-maturing oats coincide with the rainy and hot season in the northern dryland regions, making them more suitable for planting in the dryland areas of the Loess Plateau.

Effect of nitrogen and phosphorus application on starch characteristics and quality of rice with different nitrogen efficiency

IntroductionThe application of nitrogen and phosphorus fertilizers is an important factors affecting the quality of rice, and different nitrogen-efficient rice varieties show significant differences in their response to nitrogen and phosphorus application.MethodsIn this experiment, a low-nitrogen-high efficiency variety (Deyou 4727) and a high-nitrogen-high efficiency variety (Jingyou 781) were selected, and the changes in rice quality and differences in starch structure under nitrogen–phosphorus interaction treatments were determined.ResultsAppearance, flavor, starch content and thermodynamic properties, endosperm cell arrangement, and starch granule morphology and size were significantly influenced by variety, nitrogen-phosphorus interactions, and their interaction effects. The effect of nitrogen fertilizer on quality was greater than that of phosphorus fertilizer. The whiteness and chalkiness rates of Deyou 4727 first decreased and then increased with increasing nitrogen fertilizer application, wheras the appearance quality of Jingyou 781 increased with increasing nitrogen fertilizer application. Starch crystallinity in Deyou 4727 first increased and then decreased with increasing nitrogen fertilizer application, whereas starch crystallinity in Jingyou 781 increased continuously with increasing nitrogen fertilizer application. The application of phosphorus fertilizer led to an increase in starch crystallinity in both nitrogen-efficient rice varieties, consistent with the response of different rice varieties to nitrogen and phosphorus in terms of appearance and chalkiness. With the increasing application of nitrogen and phosphorus fertilizers, the differences in the physicochemical properties and structure of starch became more significant.DiscussionHigh-nitrogen-efficient rice varieties can significantly improve appearance quality under high nitrogen conditions, and the interactions of medium-high nitrogen and low-medium phosphorus can lead to a significant decrease in starch thermal parameters and retention rate, thus improving rice cooking quality. Low-nitrogen-efficient rice varieties can also improve the quality of rice under low-medium-nitrogen conditions with appropriate application of phosphorus fertilizer.

Nitrogen input reduces the physical defense of rice plant against planthopper, Nilaparvata lugens (Hemiptera: Delphacidae).

Nitrogen has important effects on plant growth and defense. Although studies on the alternation in plant chemical defense by nitrogen fertilization have been extensively reported, how it affects physical defense is poorly understood. Two rice (Oryza sativa L.) (Poales: Poaceae) varieties (LDQ7 and YLY1) were applied with varying nitrogen regimes (0.90 and 180kg ha-1) to study their physical defense against the brown planthopper (BPH) Nilaparvata lugens (Hemiptera: Delphacidae) in this study. Results of the electrical penetration graph showed that BPH searching and penetrating duration time was shortened with increasing nitrogen application. Also, the tubercle papicle of rice leaves decreased with increasing nitrogen application, while rice leaves' surface structure and waxy composition changed with increasing nitrogen application. In field experiments, BPH populations increased with the application of nitrogen fertilizer. These findings suggest that nitrogen input can affect plant-insect interactions by reducing the physical defense of plants, which provides new ideas for the organic combinations of yield increase and pest control in rice fields.

Optimizing Planting Density, Irrigation, and Nitrogen Application Can Improve Rapeseed Yield in Xinjiang’s Aksu by Reducing the Lodging Rate

This study aimed to investigate the effects of planting density, irrigation volume, and nitrogen application on the resistance of rapeseed to lodging and yield and to provide technical support for achieving high yield and lodging resistance. We employed an L9 (34) orthogonal array, different planting densities, irrigation levels, and nitrogen applications to investigate their impact on rapeseed lodging and yield. The results showed the following: (1) Irrigation had the greatest effect on rapeseed lodging. This effect was most pronounced for the combination (A3B3C2), which exhibited the most severe lodging phenomenon (90%). Planting density had the greatest effect on yield, and the optimal combination was A2B2C3, which reached 3744 kg/hm2 in 2023 and 3420 kg/hm2 in 2024. (2) The agronomic practices increased the content of lignin, cellulose, hemicellulose, crude fiber, pectin, and soluble sugar fractions in the stalks by enhancing their flexural, puncture, and stress resistance. This led to the highest yield while reducing the rate of lodging. This emphasizes the importance of agricultural practices for rapeseed lodging and yield, providing critical insights into rapeseed cultivation in the Aksu region of Xinjiang.

Effects of SPAD value variations according to nitrogen application levels on rice yield and its components

Nitrogen (N) is the most essential element for growth, development, and grain yield determination in crops. However, excessive nitrogen application can result in environmental pollution and greenhouse gas emissions that contribute to climate change. In this study, we used 158 rice genetic resources to evaluate the relationships between the soil and plant analysis development (SPAD) value and grain yield (GY) and its components. The SPAD value ranged between 30.5 and 55.8, with a mean of 41.7 ± 5.3, under normal nitrogen conditions (NN, 9 kg/10a), and between 27.5 and 52.3, with a mean of 38.6 ± 4.8, under low nitrogen conditions (LN, 4.5 kg/10a). Under NN conditions, the SPAD values were in the following order: japonica (43.5 ± 5.8), Tongil-type (41.7 ± 2.5), others (41.7 ± 5.2), and indica (38.3 ± 3.8). By contrast, under LN conditions, the SPAD values were in the following order: Tongil-type (40.4 ± 2.1), others (40.1 ± 4.5), japonica (39.6 ± 5.2), and indica (35.6 ± 3.9). The 158 genetic resources showed no correlation between SPAD and yield. Therefore, the low-decrease rate (LDR) and high-decrease rate (HDR) SPAD groups were selected to reanalyze the relationships between the surveyed traits. The SPAD values were positively correlated with 1000-grain weight (TGW) for both LDR and HDR groups (NN: 0.63, LN: 0.53), However, SPAD and GY were positively correlated only in the LDR group. For TGW, the coefficient of determination (R2) was 20% and 13% under NN and LN conditions, respectively. For GY, R2 values of 32% and 52% were observed under NN and LN conditions, respectively. Genetic resources with higher SPAD values in the LDR group exhibited the highest yield (NN: 1.19 kg/m2, LN: 1.04 kg/m2) under both NN and LN conditions. In conclusion, we selected 10 genetic resources that exhibited higher GY under both NN and LN conditions with minimal yield reductions. These genetic resources represent valuable breeding materials for nitrogen deficiency adaptation.

Optimal Water and Nitrogen Regimes Increased Fruit Yield and Water Use Efficiency by Improving Root Characteristics of Drip-Fertigated Greenhouse Tomato (Solanum lycopersicum L.)

The growth of root system directly affects the absorption and utilization of soil water and nitrogen, and understanding the responses of root characteristics to water and nitrogen regimes is thus crucial for optimizing water and nitrogen management. The root characteristics of each soil layer, i.e., root length, root surface area, and root volume, as well as fruit yield and water use efficiency of greenhouse tomato under drip fertigation in response to different irrigation levels and nitrogen rates were explored in northwest China. There were four irrigation levels, i.e., 50% ETC (W1), 75% ETC (W2), 100% ETC (W3), and 125% ETC (W4), where ETC is the crop evapotranspiration, and four nitrogen rates, i.e., 0 kg ha−1 (N1), 150 kg ha−1 (N2), 250 kg ha−1 (N3), and 350 kg ha−1 (N4). The results showed that reasonable irrigation and nitrogen regimes (W3N3) significantly increased fruit yield by 31.64% and root length, root surface area, and root volume by 45.03%, 61.24%, and 148.21% compare to W3N1, respectively. The promoting effect of increasing irrigation level on root characteristics increased with soil depth and had the greatest increases in root volume by 27.07%, 123.43%, and 211.47% for the 0–10, 10–20, and 20–30 cm soil layers, respectively. In addition, reducing irrigation level significantly increased the percentages of roots in the top soil by 29.71%, 26.77%, and 18.53% for root length, root surface area, and root volume, respectively. The reasonable nitrogen rate (N3) significantly increased fruit yield by 41.11%, water use efficiency by 34.42%, and root length, root surface area, and root volume by 40.42%, 41.44%, and 112.76%, respectively. The over-application of nitrogen (N4) reduced root characteristics of all soil layers, fruit yield, and water use efficiency. The promoting effect of increasing nitrogen rate on root length of each soil layer decreased with soil depth, by 71.01%, 48.96%, and 15.71% for 0–10, 10–20, and 20–30 cm soil layers, respectively. Irrigation level was the main factor dominating the root growth of each soil layer. The correlation analysis showed that fruit yield had significantly positive correlations with root characteristics in all soil layers, while water use efficiency had significantly positive correlations with the percentages of root length and root surface area in the 0–10 cm soil layer. In conclusion, rational water and nitrogen regimes achieved better fruit yield by promoting root growth of greenhouse tomato, and the water use efficiency of greenhouse tomato was improved by increasing the root percentage in the topsoil layer to alleviate the adverse effects under water stress conditions. This study reveals how irrigation volume and nitrogen application can enhance tomato yield and water use efficiency by regulating root characteristics and vertical root distribution, providing support for understanding the response of root systems to changes in soil water and nitrogen conditions.

Nitrogen Application and Seed Inoculation with Bradyrhizobium japonicum Bacteria Improved Yield and Quality Traits of Soybean [Glycine max (L.) Merrill] Grown as Second Crop

Nitrogen Application and Seed Inoculation with Bradyrhizobium japonicum Bacteria Improved Yield and Quality Traits of Soybean [Glycine max (L.) Merrill] Grown as Second Crop

Microbiomes-Plant Interactions and K-Humate Application for Salinity Stress Mitigation and Yield Enhancement in Wheat and Faba Bean in Egypt’s Northeastern Delta

Salinity, resulting from climate change and excessive mineral fertilization, burdens farmers and negatively impacts soil and water ecosystems in the Northeastern Nile Delta. Organic and biological approaches are crucial for addressing these issues. This study examined the effects of individual and combined inoculations with cyanobacteria, yeast, and Arbuscular Mycorrhizal Fungi (AMF), with or without K-Humate and reducing Nitrogen, phosphorus and potassium (NPK) mineral fertilizers application rates to crop quality of wheat and faba bean. In preliminary laboratory experiments, the interactive effects of these microbiomes on plant antioxidant and soil enzyme production were examined under salinity stress. Results showed that co-inoculation, especially with K-Humate, yielded superior outcomes compared to individual inoculations. These findings were validated by a field trial conducted in saline-alkaline soil in the Northeastern Nile Delta region. All biological treatments 25% of recommended doses, and enhancing salinity tolerance, increasing yield, and improving enhanced rhizosphere microbial activity, including soil enzyme activity, AMF colonization, spore density, and the total numbers of bacteria, cyanobacteria, and yeast. These effects were further amplified by K-Humate and were more pronounced with combined inoculations than with individual ones, leading to improved soil fertility and significant increases in both crop quantity and quality compared to control treatments. The triple treatment, combining cyanobacteria, yeast, and mycorrhizae in the presence of K-Humate while reducing the mineral NPK rate by 75%, achieved superior increases in the productivity of wheat grains and faba bean seeds, reaching 54.72% and 128.92%, respectively, compared to the 100% NPK mineral control. This treatment also significantly improved crop quality, with notable increases in nitrogen, potassium, phosphorus, and protein percentages in wheat grains and faba bean seeds. Microbiomes-interaction increased potassium uptake over sodium, enhancing the plant’s potassium/sodium ratio and improving salt stress tolerance. This approach reduces reliance on costly mineral fertilizers, enabling bio-organic farming in marginal lands, optimizing resource utilization, and preserving natural resources.

აზოტის გამოყენება ნავთობისა და გაზის ოპერაციებში

The article discusses several options for nitrogen application in the oil and gas industry. Nitrogen gas is useful in maintaining formation pressure in oil fields, expelling gas from the gas cap, stimulating steeply inclined formations, cyclic exploitation of gas condensate deposits, Completion of wells, Cleaning of wells with Coiled Tubing, Circulatimg low pressure reservoir wells with foam.Described experience of local oil and gas companies in Georgia utilizing nitrogen pumping in welltesting and stimulation.

Contrasting Alleles of OsNRT1.1b Fostering Potential in Improving Nitrogen Use Efficiency in Rice.

Nitrogen use efficiency (NUE) is important for the growth and development of rice and is significant in reducing the costs of rice production. OsNRT1.1b is involved in nitrate assimilation, and the alleles at position 21,759,092 on chromosome 10 clearly separate indica (Pathum Thani 1 (PTT1) and Homcholasit (HCS)) and japonica (Azucena and Leum Pua (LP)) rice varieties. Rice morphological and physiological traits were collected at three nitrogen levels (N0 = 0 kg ha-1, N7 = 43.75 kg ha-1, and N14 = 87.5 kg ha-1). Leaf and tiller numbers in PTT1 and HCS at N7 and N14 were two to three times higher than those at N0. At harvest, the biomass yield in PTT1 was the highest, while the total grain number in HCS was the maximum. The leaf widths and total chlorophyll contents (SPAD units) of Azucena and LP increased with nitrogen application as well as photosynthetic pigment parameters; for example, plant senescence reflectance indices (PSRIs), structure-insensitive pigment indices (SIPIs), and modified chlorophyll absorption ratio indices (MCARIs) were highly related in the japonica varieties. PTT1 and HCS, both carrying the A allele at OsNRT1.1b, had better NUE than Azucena and LP with the G allele. HCS, overall, had better NUE than PTT1. The translation to grain yield of assimilates was remarkable in PTT1 and HCS compared with Azucena and LP. In addition, HCS converted biomass for a 75% higher yield than PTT1. The ability of HCS to produce high yields was achieved even at N7 nitrogen fertilization, manifesting efficient use of nitrogen.

Determination of sudan III using nitrogen and sulfur co-doped carbon quantum dots with fluorescence enhancement effect

Sudan III, a synthetic azo dye, is commonly used for coloring oils, waxes, and various plastics. However, its unauthorized presence in food and food products poses serious health hazards, including potential carcinogenic effects. In this study, we investigated the application of nitrogen and sulfur co-doped carbon quantum dots (N,S-CQDs) as a fluorescence sensor to detect trace amounts of sudan III. When exposed to trace amounts of sudan III, the fluorescence signal of N,S-CQDs was significantly enhanced. Under optimal conditions (pH 5.0 and an incubation time of 4 minutes), the fluorescence enhancement of N,S-CQDs showed a linear relationship with sudan III concentration in the range of 7.5 x 10-10 to 1.0 x 10-8 M, with a limit of detection of 2.0 x 10-10 M (equivalent to 0.07 ppb). The method demonstrated high accuracy (recovery from 91.5% to 103.5%), good reproducibility (RSD < 3.86%), and excellent selectivity for sudan III over other azo dyes. We successfully applied this developed method to analyze sudan III in chili sauce and chili powder samples, cross-checking differences between results less than 10% with LC-MS/MS analysis.

Yield and Nitrogen Uptake of Wheat as Influenced by Nitrogen, Vermicompost and Biofertilizer Application at Rampur, Chitwan, Nepal

High cost, unavailability, and imbalance use of chemical fertilizers and environmental degradation are challenging sustainability of wheat production in Nepal. Integration of vermicompost, biofertilizer (Azotobacter chrococcom) and chemical fertilizer can improve the situation. A field experiment was conducted to assess the combined application of vermicompost, chemical fertilizer and biofertilizer on the growth, yield, and nitrogen uptake of wheat at Rampur Chitwan from November 2021 to April 2022. The experiment was laid out in a strip-split plot design with five N levels as horizontal factors (0, 25, 50, 75, and 100% of recommended dose), two vermicompost levels (0 and 5 t ha-1) as the vertical factor, and two biofertilizer levels (with and without application) as the sub-plot factor with three replications. Data on growth, yield attributes and yield of wheat, nitrogen uptake were collected, analyzed and presented. The research result revealed a significant influence of biofertilizer and nitrogen levels on grain yield of wheat. Full dose of nitrogen resulted a significantly higher grain yield (4441.71 kg ha-1) than other nitrogen levels (0, 25, 50, 75 % of recommended dose with grain yield 2792.11, 2982.20, 3771.17. 4231.63 kg ha-1 respectively). Biofertilizer application had significantly higher grain yield (3726.76 kg ha-1) compared with no biofertilizer application (3560 kg ha-1). There was significant interactions between biofertilizer application and nitrogen levels on number of grains per spike and sterility percent of wheat. The number of grain per spike was highest with 100% recommended N with biofertilizer application (47.52 grains/spike) followed by 75 % recommended N with biofertilizer application (46.53 grains/spike). The sterility % was lowest (6.47 %) with 100 % N with biofertilizer application followed by 75 % N with biofertilizer application (6.72 %). Total nitrogen uptake was significantly higher (119.04 kg ha-1) for full dose of nitrogen as compared with lower nitrogen levels (0, 25. 50, 75 % recommended N levels). Biofertilizer application also resulted in significantly higher nitrogen uptake (89.89 kg ha-1) as compared with no Biofertilizer application (79.89 kg ha-1). Integration of biofertilizer, with chemical fertilizer 75 - 100 % recommended N level could increase the growth, yield, and nitrogen uptake of wheat in Chitwan.