Application Of Moringa Leaf Extract Research Articles

Hermetic effect of Moringa oleifera leaf extract mitigates salinity stress in maize by modulating photosynthetic efficiency, and antioxidant activities

Salinity poses a significant constraint to cereal productivity particularly in arid and semiarid regions. The application of allelochemical has shown promising results in mitigating the intensity of abiotic stresses. A pot experiment was conducted to assess the efficacy of different concentrations of aqueous allelopathic extract derived from moringa leaves in mitigating the adverse impacts of salinity on the germination and growth of maize cultivars via seed priming. The study involved three variables: two cultivars of maize, ‘Pioneer 30Y87’ (salt tolerant) and ‘Pioneer 30T60’ (salt sensitive) e seed priming with moringa leaf extract (MLE) at varying concentrations of 0.5%, 1.0%, 1.5%, 2.0%, 2.5%, 3.0%, and hydro-priming as control; and different salinity levels of 0, 6, and 12 dS m-1. Salinity had a negative impact on the germination process, leading to delayed and suboptimal growth of seedlings. Additionally, salinity reduced the synthesis of photosynthetic pigments (20-50%), photosynthesis, transpiration, internal carbon, and stomatal conductance. Further, MLE also improved the antioxidant activities (catalase: CAT and peroxidase: POD) by 22-56% which reduced the hydrogen peroxide production. Moreover, ‘P-30Y87’ exhibited favorable performance in terms of better germination, growth, photosynthesis and antioxidant activities. The application of moringa leaf extract (3%) resulted in a more notable hermetic effect in elevating salinity stress thereby enhancing germination, growth, photosynthesis and antioxidant activities. In the conclusion, application of MLE (3%) is a promising approach to mitigate the adverse impacts of salinity by improving germination, growth, photosynthesis and antioxidant activities.

Physical and chemical properties of green chili pepper during storage in response to pre- and post-harvest application of moringa leaf extract

ABSTRACT The primary challenge encountered by marketers of green chili peppers is the rapid post-harvest deterioration. The application of pre- and post-harvest moringa leaf extract (MLE) is an effective approach to preserving the quality of fruits after being harvested. This study aimed to assess the effect of MLE application at pre- and post-harvest periods on physical and chemical properties of green chili during storage. For this purpose, a completely randomized design was arranged with nine replications. Pre-harvest MLE (seed soaking + foliar spraying with MLE) treatment showed a similar response to the control. Meanwhile, post-harvest MLE application on fruit by dipping method had better results on L*, a*, weight loss, firmness, and vitamin C after 7 d of storage compared to the control. These results suggested that post-harvest MLE application was more effective in prolonging the self-life of green chili, particularly in maintaining the physical attributes.

Mitigating the Toxic Effects of Salinity in Wheat Though Exogenous Application of Moringa Leaf Extract

Mitigating the Toxic Effects of Salinity in Wheat Though Exogenous Application of Moringa Leaf Extract

Enhancing sweet potato (Ipomoea batatas) resilience grown in cadmium-contaminated saline soil: a synergistic approach using Moringa leaf extract and effective microorganisms application

Raising soil contamination with cadmium (Cd2+) and salinization necessitates the development of green approaches using bio-elicitors to ensure sustainable crop production and mitigate the detrimental health impacts. Two field trials were carried out to study the individual and combined effects of foliage spraying of Moringa leaf extract (MLE) and soil application of effective microorganisms (EMs) on the physio-biochemical, osmolytes, antioxidants, and performance of sweet potato grown in Cd2+-contaminated salty soil (Cd2+ = 17.42 mg kg−1 soil and soil salinity ECe = 7.42 dS m−1). Application of MLE, EMs, or MLE plus EMs significantly reduced the accumulation of Cd2+ in roots by 55.6%, 50.0%, or 68.1% and in leaves by 31.4%, 27.6%, or 38.0%, respectively, compared to the control. Co-application of MLE and EMs reduced Na+ concentration while substantially raising N, P, K+, and Ca2+ acquisition in the leaves. MLE and EMs-treated plants exhibited higher concentrations of total soluble sugar by 69.6%, free proline by 47.7%, total free amino acids by 29.0%, and protein by 125.7% compared to the control. The enzymatic (SOD, APX, GR, and CAT) and non-enzymatic (phenolic acids, GSH, and AsA) antioxidants increased in plants treated with MLE and/or EMs application. Applying MLE and/or EMs increased the leaf photosynthetic pigment contents, membrane stability, relative water content, water productivity, growth traits, and tuber yield of Cd2+ and salt-stressed sweet potato. Consequently, the integrative application of MLE and EMs achieved the best results exceeding the single treatments recommended in future application to sweet potato in saline soil contaminated with Cd2+.

Exogenous application of bio-stimulants and growth retardants improve nutrient absorption and fiber quality in upland cotton

BackgroundNatural and synthetic plant growth regulators are essential for plant health, likewise these regulators also play a role in increasing organic production productivity and improving quality and yield stability. In the present study, we have evaluated the effects of foliar applied plant growth regulators, i.e., moringa leaf extract (MLE) and mepiquat chloride (MC) alone and in combination MC and MLE on the conventional cotton cultivar (CIM 573) and transgenic one (CIM 598). The growth regulators were applied at the start of bloom, 45 and 90 days after blooming.ResultsThe application of MC and MLE at 90 days after blooming significantly improved the relative growth rate, net assimilation rate, the number of bolls per plant, and seed cotton yield. Likewise, the combined application of MLE and MC at 90 days after blooming significantly boosted the nitrogen uptake in locules, as well as the phosphorus and potassium uptake in the leaves of both cotton cultivars. The application of MLE alone has considerably improved the nitrogen uptake in leaves, and phosphorus and potassium contents in locules of Bt and conventional cotton cultivars. Similarly, Bt cotton treated with MLE at 90 days after blooming produced significantly higher ginning out turn and oil contents. Treatment in combination (MLE + MC) at 90 days after blooming produced considerably higher micronaire value, fiber strength, and staple length in conventional cultivar.ConclusionThe natural growth enhancer, MLE is a rich source of minerals and zeatin, improving the nutrient absorption and quality of cotton fiber in both conventional and Bt cotton cultivars.

RESPONSE OF STRAWBERRY GROWTH, YIELD AND MARKETABLE FRUIT QUALITY TO SPRAYING WITH MORINGA LEAF EXTRACT, CALCIUM AND POTASSIUM SILICATE

Tow field and storage experiments were carried out to evaluate the effect of foliar application of moringa leaf extract, calcium, and potassium silicate on the growth, yield, and quality characteristics of strawberry fruits. The field experiment included 27 treatments: Foliar application of moringa leaf extract at the concentrations of 0, 5, and 10% which were symbolized as M0, M1, and M2 respectively, calcium at the concentrations of 0, 1, and 2 g. L-1, which were symbolized as Ca0, Ca1, and Ca2 respectively, and potassium silicate at the concentrations of 0, 1.25, and 2.50 ml. L-1 which were symbolized as S0, S1, and S2 respectively, with three replicates. The storage experiment was carried out according to the same design that was applied in the first experiment, at a temperature of 0-2 ºC. The results revealed that the M2 treatment significantly increased Plant height, leaf number, crown diameter, flowering cluster number, plant yield, Fruit T.S.S, Vitamin C, as well as maintaining the T.S.S and Vitamin C after storage, and reduced the fruits distortion. The treatment of Ca1 recorded a significant increment in the growth and yield parameters and reduced fruit distortion, Also the treatment of S2 recorded significant values of the above-mentioned parameters, Moreover, the interactions of M2Ca1, M2S2, and Ca1S2 and the triple interaction of M2Ca1S2 recorded the highest values of the studied parameters.

Enhancing Wheat Tolerance to Cadmium Stress through Moringa Leaf Extract Foliar Application.

Cadmium, a hazardous heavy metal prevalent in plants and soil, poses a significant threat to human health, particularly as approximately 60% of the global population consumes wheat, which can accumulate high levels of Cd through its roots. This uptake leads to the translocation of Cd to the shoots and grains, exacerbating the potential health risks. However, promising results have been observed with the use of moringa leaf extract (MLE) foliar spray in mitigating the adverse effects of Cd stress. The current experiment was conducted to find out the Cd stress tolerance of wheat varieties V1 = Akbar-19 and V2 = Dilkash-2020 under exogenous spray of MLE. The treatments of this study were T0 = 0% MLE + 0 µM Cd, T1 = 3% MLE + 0 µM Cd, T2 = 0% MLE + 400 µM Cd, and T3 = 3% MLE + 400 µM Cd. Cd stress demonstrated a significant reduction in morphological attributes as shoot and root fresh weight (22%), shoot and root dry weight (24.5%), shoot and root length (22.5%), area of leaf and number of leaves 30.5%, and photosynthetic attributes (69.8%) in comparison with control. Exposure of wheat plants to Cd toxicity cause oxidative stress, increased H2O2, and MDA up to 75% while foliar application of MLE reduced the activities of reactive oxygen species (ROS). The activity of catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), and ascorbic acid (AsA) increased up to 81.5% as well as organic osmolytes such as phenolics, total soluble proteins, and total soluble sugars were improved up to 77% by MLE applications under Cd stress. Higher accumulation of ionic contents root Na+ (22%) and Cd (44%) was documented in plants under Cd stress as compared to control, while uptake of root mineral ions Ca2+ and K+ was 35% more in MLE-treated plants. In crux, Cd toxicity significantly declined the growth, photosynthetic, and biochemical parameters while 3% MLE application was found effective in alleviating the Cd toxicity by improving growth and physiological parameters while declining reactive oxygen species and root Na+ as well as Cd uptake in wheat.

Impact of Foliar-Applied Plant Extracts on Growth, Physiological and Yield Attributes of the Potato (Solanum tuberosum L.)

The current reliance on pesticides and synthetic fertilizers has been vital to sustain and increase agricultural production. The continuous, excessive use of these traditional practices has negatively affected consumer health and burdened the ecosystem. The use of plant extracts as a tool to minimize agrochemical inputs has been extensively investigated; these extracts have the ability to improve both plant growth and agricultural productivity. Therefore, this study was conducted to determine the effect of foliar plant extract application on potato growth, as well as on certain physiological and yield attributes. From four weeks after planting onwards, five healthy, equal-sized potato plants per treatment received various foliar plant extract applications. These extracts included brown seaweed Ascophyllum nodosum extract (ANE), aloe vera leaf extract (AVE), garlic bulb extract (GBE), and moringa leaf extract (MLE). The treatments were repeated weekly until harvesting. Application data regarding growth and physiological parameters were collected weekly. The pre-harvest foliar application of various plant extracts significantly enhanced (p ≤ 0.05) the plant growth, physiological, and yield attributes of potatoes. The best growth and yield responses were observed following ANE and MLE application. Plant extracts have shown beneficial effects on other crops as well, but further validation of these effects is still necessary in order to popularize and commercialize such applications.

Moringa leaf extract increases tolerance to salt stress, promotes growth, increases yield, and reduces nitrate concentration in lettuce plants

The use of large amounts of synthetic chemicals in plant cultivation harms the environment and reduces food quality. Therefore, natural alternatives should be used to protect the environment, increase food quality, and decrease food contamination. This study investigated the effect of moringa leaf extract (MLE) as a plant stimulator to enhance growth, yield, and quality of lettuce grown in a saline environment. To examine the effect of application method and extract concentration, this experiment included three levels of seed priming (0, 5 %, and 10 % MLE) and three levels of foliar spray (0, 5 %, and 10 % MLE). MLE application, either by seed priming or foliar spraying, reduced the adverse effects of salt stress and increased lettuce growth, yield, and quality. However, the combined treatment of 5 % seed soaking and 10 % foliar spray showed the best results in increasing antioxidant levels (catalase (CAT), ascorbate peroxidase (APX), and ascorbic acid), relative water content (RWC %), nutrient concentration (N, P, and K), and chlorophyll content, and maintaining membrane integrity by reducing electrolyte leakage (EL %) and sodium accumulation, leading to improved yield parameters (head diameter, head weight, and total yield), and increasing lettuce head quality by reducing nitrate concentration. The results suggest that MLE (5 % seed soaking and 10 % foliar spray) is a practical, effective, and inexpensive approach to alleviate salt stress and improve lettuce yield and quality under saline conditions. However, despite the observed positive effects, further studies are needed to understand the factors determining the response of plants to MLE application.

Moringa oleifera: A Boon to Agriculture

Moringa (Moringa oleifera Lam.), also known as the horseradish tree, drumstick, or sajna, is a multipurpose tree that is found around the world in tropical and subtropical climate zones. Having various medicinal and nutrition properties, it is also known as miracle tree. This review study was done for easy access and dissemination of scientifically proven and culturally practiced uses of moringa tree to the agricultural group of Nepal. Different information and data used in this paper were taken from the multiple journals found in online platform. The leaves of the moringa tree are particularly beneficial since they are rich in protein, calcium, iron, and vitamin C, while the bark aids in the absorption of heavy metals. The moringa plant’s leaf extracts have a number of beneficial effects, including anticancer, antibacterial, and antifungal characteristics. Making fermented plant juice from leaves and seeds can be used as organic fertilizer in agriculture. Application of moringa leaf extract throughout the booting, milking and heading stages significantly reduces wheat aphid populations. To control pests in stored grain, extracts from different moringa tree parts are employed. Moringa oleifera seed methanolic extract can be used to control Callosobruchus maculatus and Sitophilus oryzae on their respective grains. Application of the growth hormone present in moringa leaf extract is said to boost crop output by 45%. Due to its good green manure and fencing capacity, moringa plays crucial function in agroforestry system. Therefore, moringa is a multipurpose tree because of its benefits to agriculture, environment and human health.

Exogenous Application of Moringa Leaf Extract Confers Salinity Tolerance in Sunflower by Concerted Regulation of Antioxidants and Secondary Metabolites

Exogenous Application of Moringa Leaf Extract Confers Salinity Tolerance in Sunflower by Concerted Regulation of Antioxidants and Secondary Metabolites

Assessment of Biostimulant Derived from Moringa Leaf Extract on Growth, Physiology, Yield, and Quality of Green Chili Pepper

With the rapid growth in global population and standards of living, improving food production and quality are the greatest challenges in agriculture. The application of biostimulant derived from moringa leaf extract (MLE) has attracted a great deal of interest to support these efforts in a sustainable approach. A field study was conducted using a randomized complete block design with four replications. The effect of individual and combined application of MLE through seed priming (seed soaking) and foliar spray at different rates (1:30, 1:20, and 1:10, v/v) on growth, physiological, yield, and quality traits of green chili pepper were investigated, which was the aim of this study. In general, the responses generated by the combined MLE methods were more pronounced compared to their single applications. Among all treatments, MLE priming plus foliar spray at 1:30 was effective in improving most traits observed, including chlorophyll fluorescence (6.49%), stomatal conductance (57.19%), plant height (30.57%), leaf number (88.89%), leaf area index (116.67%), fruit weight per plant (46.27%), average fruit weight (39.62%), length (9.89%), diameter (29.65%), firmness (27.77%), and vitamin C content (29.07%) of fruit. Therefore, it is regarded as an appropriate treatment to maximize the potential use of MLE in green chili.

Foliar application of moringa leaf extract (MLE) enhanced antioxidant system, growth, and biomass related attributes in safflower plants

The use of synthetic fertilizers is a potential source of environmental hazards. Natural plant-based growth regulators are eco-friendly, inexpensive and their use is highly recommended. Moringa (Moringa oleifera L.) is also known as a miracle plant with high nutritional content, medicinal and plant growth-promoting properties due to the presence of appreciable levels of hormones, vitamins, micro, and macronutrients. However, no literature has been found regarding the effect of moringa leaf extract (MLE) application on safflower plants (Carthamus tinctorius L.). Therefore, the present experiment investigated the effect of two different concentrations of MLE (3 and 20%) on various biochemical, mineral content, growth, and biomass-related attributes of safflower plants. Results revealed that application of MLE significantly improved chlorophyll content, maximum quantum yield of photosystem II (Fv/Fm), shoot and root length, fresh and dry biomass. Moreover, MLE application also significantly enhanced activities of leaf superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) while decreasing H2O2 and MDA content. Foliar application of MLE also resulted in a notable increase in proline, glycine betaine, and mineral content. The application of MLE (20%) manifested maximum improvement in all attributes when compared to MLE (3%). Pearson's correlation analysis revealed positive correlation among growth, biomass, biochemical and mineral content, whereas a strong negative correlation was found between these traits and oxidative stress markers (H2O2 and MDA) under MLE application. The present experimental findings indicate that MLE application can be employed as a natural plant growth regulator. Furthermore, the MLE enhanced the antioxidant system and reduced oxidative stress, therefore, it can be used to ameliorate the detrimental effects of environmental adversities on plants.

Assessing the Usefulness of Moringa oleifera Leaf Extract as a Biostimulant to Supplement Synthetic Fertilizers: A Review.

The extensive use of synthetic chemical fertilizers is associated with environmental pollution and soil degradation. In addition, the high costs of these fertilizers necessitate the search for alternative, eco-friendly and safe natural sources of phytonutrients. The liquid extracted from moringa (Moringa oleifera Lam.) leaves has been used in agriculture to improve the growth and productivity of several crops. The efficacy of moringa leaf extract (MLE) is attributed to its high content of mineral nutrients, protein, vitamins, sugars, fiber, phenolics and free proline. In addition, MLE contains significant amounts of phytohormones, such as auxins, cytokinins and gibberellins. Furthermore, MLE is a valuable product promoting seed germination, plant growth and deeper root development, delaying fruit senescence and increasing the yield and quality of crops grown under normal or stressful conditions. Here, we review the research on MLE as a biostimulant to enhance crop growth and productivity. Moreover, we emphasize its possible introduction to smallholder farming systems to provide phytonutrients, and we further highlight research gaps in the existing knowledge regarding MLE application. Generally, MLE is an inexpensive, sustainable, eco-friendly and natural biostimulant that can be used to improve the growth and productivity attributes of various crops under non-stressful and stressful conditions.

Retraction: Exogenous application of moringa leaf extract improves growth, biochemical attributes, and productivity of late-sown quinoa

Retraction: Exogenous application of moringa leaf extract improves growth, biochemical attributes, and productivity of late-sown quinoa

Exploring the Potential of Moringa Leaf Extract for Mitigation of Cadmium Stress in Triticum aestivum L.

Cadmium (Cd) is one of the most toxic metals accumulated in wheat grains. Daily intake of Cd through food is posing serious health problems. There is an urgent need to reduce the uptake and accumulation of Cd in wheat and other cereal crops. In this study, we investigated the potential of moringa leaf extract (MLE) in decreasing the Cd toxic effects in wheat (Triticum aestivum L.) cv. A.S. 2002. Sowing was carried out in pots under shade and natural light conditions. Two Cd concentrations (500 µM and 1000 µM) were applied with and without MLE (0 and 3%). Results revealed that plant growth parameters viz diameter of stem, number of leaves, leaf area, photosynthetic pigments were improved with MLE application under Cd stress. Moreover, biochemical attributes and osmolytes such as total soluble sugars, and soluble proteins, phenolic content and flavonoids were negatively effected by Cd stress and were improved under MLE treatment. Moreover, Cd stress enhanced phytotoxicity as higher ROS accumulation is observed under both stress conditions, whereas after MLE application MDA and H2O2 accumulation was significantly decreased. Based on current observations, MLE was effective in mitigating the biological and toxic effects of Cd by promoting the growth attributes and mineral contents in wheat. Further experiments are needed for discovering the molecular mechanisms underlying MLE and host plant interactions involved in Cd stress mitigation.

English

Salinity is major abiotic stress limiting plant growth worldwide. Plant adaptation to salinity stress involves diverse physiological and metabolic pathways. In this study, we assessed the effects of foliar application of zinc oxide nanoparticles (ZnONPs) and Moringa leaf extract (MLE) on salt tolerance in faba beans (cultivar, Sakha 4). Morphological, chemical, and biochemical parameters of plants grown under saline condition (50 and 100 mM NaCl) were assessed 60 days after sowing. Salt stress caused a remarkable reduction in growth traits, photosynthetic pigments, proline, minerals, total phenol, and enzyme activity of the faba bean variety. The results showed that foliar spraying of MLE and ZnONPs on faba bean grown under salt-stressed conditions promoted growth parameters (that is, shoot length, numbers of leaves, relative water content, shoot, roots fresh and dry weights), photosynthetic pigments (that is, chl a, b, total chlorophyll and carotenoids), proline, mineral elements (Na+, K+, Ca+2, and Zn+2), total phenol and enzyme activity (POX, PPO, APX, and CAT) compared to control plants. Based on these findings, the potential of foliar spraying application of MLE and ZnONPs may help alleviate the negative effect of salinity on growth, photosynthesis efficiency, and biochemical properties of faba bean.   Key words: Faba bean, Moringa oleifera, antioxidant enzyme activity, ZnO nanoparticles, salt stress, proline.

Effect of foliar application of moringa leaf extract on growth and fruit yield of Capsicum annuum L. (chilli) cv. MIPC-1

This study was carried out in the Crop Farm of Eastern University, Sri Lanka during the period from January to May 2019 to ascertain the effect of foliar application of moringa leaf extract (MLE) on growth and fruit yield of Capsicum annum L.cv (chilli) MIPC-1. This investigation was followed in a pot experiment with Completely Randomized Design (CRD) with seven treatments and seven replicates. The concentrations of Moringa leaf extract were 10%, 20%, 30% in addition to 0% (distilled water) as control. The treatments included were; T0 - control (Distilled water), T1 - 10% MLE at once a week interval, T2 - 10% MLE at once in two weeks interval, T3 - 20% MLE at once a week interval, T4 - 20% MLE at once in two weeks interval, T5 - 30% MLE at once a week interval and T6 - 30% MLE at once in two weeks interval. The Moringa oleifera leaf extract was sprayed on leaves and axial parts starting from two weeks after transplanting (WAT) and it was continued until pod formation. 25 ml of MLE was sprayed per plant. The results of the experiment showed that the foliar application of MLE with 10% concentration at one-week interval had significant (p<0.05) effects on the plant height, number of branches/plant, dry weight of leaves/plant, stems/plant, roots/plant, fruit/plant and total dry of plant were statistically analysed using Statistically Analytical Software (SAS). The results indicated that the foliar application of moringa leaf extract stimulated the growth and fruit yield of chilli. Based on the results, it was concluded that MLE helps in improving the growth and fruit yield of Chilli and MLE with 10% concentration at one-week interval is recommended for improved growth and fruit yield of Capsicum annuum L.cv.(Chilli) MIPC-1.

Application of natural and synthetic growth promoters improves the productivity and quality of quinoa crop through enhanced photosynthetic and antioxidant activities

Modern agriculture is primarily concerned with enhanced productivity of field crops linked with maximum resources use efficiency to feed the increasing population of the world. Exogenous application of biostimulants is considered a sustainable and ecofriendly approach to improve the growth and productivity of agronomic and horticultural field crops. The present study was carried out to explore the comparative growth enhancing potential of plant biostimulants (moringa leaf extract at 3% and sorghum water extract at 3%) and synthetic growth promoters (ascorbic acid at 500 μM and hydrogen peroxide at 100 μM) on growth, productivity and quality of quinoa crop (cultivar UAF-Q7) because it has gained significant popularity among agricultural scientists and farmers throughout the world due to its high nutritional profile. A field experiment was carried out at the Research Area of Directorate of Farms, University of Agriculture, Faisalabad, Pakistan during quinoa cultivation season of 2016–2017 and repeated during next year (2017–2018). All the foliar treatments enhanced the physiological, biochemical, quality, growth and yield attributes of quinoa as compared to control group. However, maximum improvement was observed in chlorophyll a and b contents, photosynthesis and respiration rates, and water use efficiency by moringa leaf extract (MLE) application. MLE application was also found more responsive regarding the improvement in activities of peroxidase, catalase, superoxide dismutase, phenolics and glycine betaine as compared to other treatments. Mineral elements i.e. K, Ca and N in root as well as in shoot were found the highest in response to MLE application. Similarly, growth (plant fresh and dry biomass, plant length and grain yield) and grain quality parameters (protein, K and Ca) were also significantly enhanced. Application of MLE was found to be a viable approach to improve the growth and quality of produce as compared to synthetic compounds.

The Impact of Bio-Stimulants on Cd-Stressed Wheat (Triticum aestivum L.): Insights Into Growth, Chlorophyll Fluorescence, Cd Accumulation, and Osmolyte Regulation.

It has been established that wheat (Triticum aestivum L.) has a higher Cd absorption capacity than other cereal crops causing an excess daily Cd intake and a huge threat for public health. Therefore, the reduction of Cd accumulation in wheat from the soil is a crucial food-security issue. A pot trial was performed on Cd-stressed wheat seedlings to evaluate the morphological and physio-biochemical responses via foliage spray of two different bio-stimulants, i.e., ascorbic acid (AsA) and moringa leaf extract (MLE). Two wheat cultivars (Fsd-08 and Glxy-13) were exposed to cadmium (CdCl2.5H2O) stress (0, 500, and 1,000 μM), along with foliar spray of AsA (0 and 50 mM) and MLE (0 and 3%). The most observable growth reduction was documented in plants that are exposed to a higher Cd concentration (1,000 μM), followed by the lower Cd level (500 μM). The wheat growth attributes, such as number of leaves per plant, number of tillers per plant, biomass yield, shoot/root length, and leaf area, were greatly depressed under the Cd stress, irrespective of the cultivar. Under the increasing Cd stress, a significant diminution was observed in maximum photochemical efficiency (Fv/Fm), photochemical quenching (qP), and electron transport rate (ETR) accompanied with reduced gas exchange attributes. However, Cd-induced phytotoxicity enhanced the non-photochemical quenching (NPQ) and internal carbon dioxide concentration (Ci), which was confirmed by their significant positive correlation with Cd contents in shoot and root tissues of both cultivars. The contents of proline, AsA, glycine betaine (GB), tocopherol, total free amino acid (TFAA), and total soluble sugar (TSS) were greatly decreased with Cd stress (1,000 μM), while MLE and AsA significantly enhanced the osmolytes accumulation under both Cd levels (especially 500 μM level). The Cd accumulation was predominantly found in the root as compared to shoots in both cultivars, which has declined after the application of MLE and AsA. Conclusively, MLE was found to be more effective to mitigate Cd-induced phytotoxicity up to 500 μM Cd concentration, compared with the AsA amendment.