Application Of Leaf Extract Research Articles

Efficacy of moringa (Moringa oleifera) formulations on suppression of root-knot nematodes (Meloidogyne incognita) and growth of tomato (Solanum lycopersicum)

Root-knot (Meloidogyne sp.) nematodes are among the most important causes of reduced tomato (Solanum lycopersicum) yield. Therefore, an investigation was conducted in 2022 at the Aquaculture Research Unit lab to evaluate the effectiveness of moringa (Moringa oleifera) leaf extract application in controlling root-knot nematode in tomatoes. During a survey of a tomato field, M. incognita was identified from the rhizosphere soil samples. Fresh leaf extracts of moringa, including positive control (fenamiphos 0.06), were investigated in vivo for efficacy against M. incognita in root and soil. Seedlings of tomato were inoculated with 3000 eggs and second-stage juveniles (J2s) of M. incognita in pot trials, with treatments comprising fresh leaf extracts of moringa in a series of concentrations (40 ppm-80 ppm). Fifty-six days after inoculation, the phytonematicide had reduced eggs and J2s in soil by 65-90%, gall number by 45-87%, and gall index by 35-82%. The best treatment was moringa leaf extract (80 ppm), which was significantly different (P less than 0.05) from moringa leaf extract at 60 ppm and 40 ppm (P less than 0.05). However, fenamiphos had no significant difference (P greater than 0.05). The results showed moringa leaf extract (80 ppm) was the best treatment for controlling root-knot nematode in tomatoes.

Moringa Leaf Extract Mitigates the Adverse Impacts of Drought and Improves the Yield and Grain Quality of Rice through Enhanced Physiological, Biochemical, and Antioxidant Activities.

Agriculture, around the globe, is facing great challenges including the need to increase the production of nutrient-dense food and to withstand climate change's impact on water and soil conservation. Among these challenges, drought stress is considered the most overwhelming danger for the agriculture sector. Organic plant growth ingredients are frequently used to enhance the growth and production of field crops cultivated in normal and unfavorable conditions. The present study was designed to explore whether leaves extracted from various landraces of Moringa could play a defensive role against drought stress in rice. Seedlings were grown under three water conditions, i.e., normal conditions (control; 100% field capacity), moderate (75%), and severe drought (50%). Leaf extracts obtained from four Moringa landraces were used as foliar spray at the tillering, panicle initiation, and grain filling stages of cultivating rice plants. The levels of water stress negatively influenced photosynthetic pigment synthesis, gas exchange traits, antioxidant activities, and yield and grain quality parameters. Leaf extracts, at the rate of 3%, from all the landraces significantly enhanced the biochemical, physiological, and yield-related attributes of rice plants under normal and unfavorable growth conditions. Particularly, leaf extract from the Faisalabad landrace was the most effective biostimulant to increase photosynthetic (8.2%) and transpiration (13.3%) rates, stomatal conductance (8.3%), chlorophyll a (15.9%) and b (9.7%) contents, and carotenoids (10.4%) as compared to water spray. The maximum photosynthesis rate was observed at 14.27 µmol CO2 m-2 s-1 via application of leaf extract from the Faisalabad landrace followed by the DG Khan (13.92 µmol CO2 m-2 s-1) and Multan (13.9 µmol CO2 m-2 s-1) landraces, respectively. Improved grain yield (25.4%) and grain quality (an increase of 10.1% in amylose with a decrease of 2.8% in amylopectin) in rice plants along with enzymatic activities such as catalase (21.2%), superoxide dismutase (38.6%), and ascorbate peroxidase (24.3%) were observed at the peak after application of leaf extract from the Faisalabad landrace. The maximum grain yield of 53.59 g per plant was recorded when using Faisalabad landrace leaf extract and the minimum (40 g) using water spray. It is concluded from the findings of the current experiment that leaf extract from the Faisalabad landrace possesses higher biostimulant potential than other landraces and can be applied to mitigate the adverse impacts of drought stress with higher productivity and improved grain quality of rice.

Extracts obtained from organs of saffron (Crocus sativus) alter growth and seed germination of common lambsquarters (Chenopodium album) and barnyard grass (Echinochloa crus-galli)

With the aim of examining the allelopathic effects of saffron (Crocus sativus), Chenopodium album and Echinochloa crus-galli were selected for this study. The experiment was carried out in different levels using aqueous extract of different organs (leaf, corm and a combination of leaf and corm) with different concentrations of the extracts (0, 1, 2.5, 5, 10 and 20%). The results showed that seed germination of both weeds was significantly affected by the organs and concentration of saffron extracts. The highest germination rate of C. album was 2.38 seeds per day in the application of corms extract and the lowest germination rate was 2.12 seeds per day in the application of combination of leaf and corm extract. The highest seed vigor index of C. album was 12 in the application of leaf extract and the lowest was 9 in the application of leaf and corm extracts. In addition, the C. album application of the highest concentration of the extract (20%) led to the lowest weight of root, stem, seedlings, germination percentage, germination rate, seed vigor index, and number of normal seeds. In E. crus-galli, the increase in the concentration of the extract from 0 to 20% enhanced the mean germination time from 3.4 in control sample to 4.1 days with 20% extract concentration. All the applied extracts obtained from different saffron organs caused a significant reduction in germination traits in both species of weeds. Meanwhile the extracts of leaf and corm exerted the highest effects. Generally, the results showed that the application of 20% extract obtained from the combination of leaf and corm tissue provided the highest growth inhibition and lowest germination rate in both weeds.

Foliar Application of Leaf Extracts of Glycyrrhiza uralensis Increases Growth and Nutritional Value of Chinese Flowering Cabbage Plants under Field Conditions

This study was conducted to explore the effects of ethanolic extracts of leaves of Glycyrrhiza uralensis on the growth and nutritional quality of Chinese flowering cabbage (Brassica rapa subsp. parachinensis) under field conditions. Preliminary greenhouse experiments were carried out with different concentrations of G. uralensis extracts to select the suitable dose for field studies. An extract concentration of 12.5 g/L was selected based on relative growth rate analysis and increase in photosynthetic pigment biosynthesis. Shoot length, shoot fresh weight and dry weight, number of leaves, and marketable value of Chinese flowering cabbage plants were significantly increased in the field trials with foliar application of G. uralensis leaves extracts at a concentration of 12.5 g/L. This biotic elicitor also enhanced the total phenolic and flavonoid contents, with optimal values increasing by 18.76% and 22.43%, respectively, compared with the control under field conditions. The total glucosinolate content was effectively increased (from 11.21 to 15.37 μmol·g−1 DW sinigrin equivalents), particularly 4-methoxyglucobrassicin (from 4.31 to 6.72 μmol·g−1 DW sinigrin equivalents) and glucoalyssin (from 0.14 to 0.19 μmol·g−1 DW sinigrin equivalents) compared with the control plants in field trials. Overall, foliar application of leaf extracts of G. uralensis can markedly increase the growth of Chinese flowering cabbage and enhance its medicinal and nutritional quality in the fields.

Effects of salicylic acid, putrescine and moring leaf extract application on storability, quality attributes and bioactive compounds of plum cv. 'Golden Japan'

Background: Plum fruits constitute a good source of natural antioxidant substances. Particularly, plums contain large amounts of phenolic compounds and flavonoids that have natural antioxidant activity which is useful to human health. The study aimed to evaluate the effects of foliar sprays with salicylic acid (SA), putrescine (PUT) and moringa leaf extract (MLE) on the fruit quality attributes and bioactive compounds of 'Golden Japan' plums under cold storage conditions. Plum trees were sprayed twice; at fruit set stage and one month later during seasons 2018 and 2019 by combinations from SA (3 and 4 mmol/L), PUT (3 and 4 mmol/L) and MLE (5 and 10%), as well as distilled water (control). Fruits were harvested at maturity stage and stored at 0°C with relative humidity 85-90% for eight weeks. Results: With advance storage period, fruit weight loss, total soluble solids (TSS), total carotenoids content (TCC) and total phenolics content (TPC) increased significantly while the fruit firmness, lightness (L*), hue angle (h°) of colour, titratable acidity (TA), total flavonoids content (TFC) and antioxidant activity (AA) decreased significantly (P < 0.05). Statistically significant differences were observed between different treatments in maintenance on all measured parameters when compared to control. At the same time, a combined SA at 3mmol/ L, PUT at 4 3mmol/ L and MLE at 10% treatment was found to be more effective than other treatments in decreasing the weight loss, softening and maintaining titratable acidity, total carotenoids, total phenolics, total flavonoids and antioxidant activity in plum fruits during storage at 0 °C.Conclusion: It was concluded that preharvest treatment of plum fruits with salicylic acid, putrescine and moringa leaf extract was effective in delaying the ripening processes and can be used commercially to extend the storage life of postharvest plum fruits with acceptable fruit quality.

Keragaan Hama, Penyakit, dan Musuh Alami pada Budidaya Padi Organik

Organic rice cultivation intents to minimize the use of inorganic fertilizers and chemical pesticides, but in fact not apart from the disruption of pests and diseases. The study aimed to determine the pests, diseases, and natural enemies on organic rice cultivation. The research was conducted at Screen Field of ICRR at WS 2015/2016 and DS 2016, using Split Plot research design with three replications. The main plot was varieties of: 1). Inpari 25, 2). Inpari 33, and 3). Sintanur. The sub-plot was application of leaf extract of: 1). Mindi, 2). Soursop, 3). Mahogany, and 4). Control. Applications were performed on 4, 6, 8, and 10 weeks after planting (WAP). Observations of pests, diseases, and natural enemies on 5, 7, 9, and 11 WAP as many as 20 clumps/plots. The results showed that varieties and leaf extract applications significantly affected on severity of stem rot disease at 5 and 7 WAP in WS 2015/2016. In the rainy season found Yellowing on Inpari 25 and Inpari 33 varieties. However, Sintanur was more resistant to Yellowing with LCC value on two growing seasons was highest compared to the other varieties. Sintanur had the lowest Cercospora Leaf Spot disease severity compared to the other varieties. The severity of disease generally increased with the age of rice increase, while the pest population density and natural enemies were fluctuate. The density of the pest was quite high in the control plot, while the natural enemy density was high enough in the application plots of Mindi, Mahogany, and Soursop extracts.

Stimulatory effect on pea of Typha Angustifolia L. extracts and their chemical composition

ABSTRACTIn this study, the influence of aqueous and organic extracts of different plant parts (flowers, leaves, and stems) of Typha angustifolia on the germination and early seedling growth of field pea (Pisum sativum L.) was evaluated. Chemical composition of extracts of different plant parts of Typha was also determined. Aqueous (20, 40, 60, 80, and 100 g L−1) and organic extracts (at 0.5, 1, and 2 mg mL−1) were applied to the seeds of two pea cultivars, Douce de Provence and Lincoln, placed in Petri dishes. Application of extracts had a beneficial effect on germination and early seedling growth of both pea cultivars. However, aqueous extract of leaves showed the most beneficial effect at 60 and 40 g L−1 for the cultivars Douce de Provence and Lincoln, respectively. The effect could be attributed to the allelochemicals present in the aqueous extracts. Petroleum ether and chloroform extracts of leaves had the most stimulating effect on the germination and early seedling growth of pea. Analysis of Typha extracts indicated the presence of vitamin E in leaves, which could be responsible forthis stimulation. Moreover, Typha leaves also had substantial amount of flavonoids. In conclusion, the allelopathic activity of of Typha was dependent on the plant part, the solvent nature, the concentration of the extracts tested, and on the pea cultivar. Application of leaf extract was the most effective in improving the germination rate and early seedling growth of pea.

Optimization of ELISA Conditions to Quantify Colorectal Cancer Antigen-Antibody Complex Protein (GA733-FcK) Expressed in Transgenic Plant

The purpose of this study is to optimize ELISA conditions to quantify the colorectal cancer antigen GA733 linked to the Fc antibody fragment fused to KDEL, an ER retention motif (GA733-FcK) expressed in transgenic plant. Variable conditions of capture antibody, blocking buffer, and detection antibody for ELISA were optimized with application of leaf extracts from transgenic plant expressing GA733-FcK. In detection antibody, anti-EpCAM/CD362 IgG recognizing the GA733 did not detect any GA733-FcK whereas anti-human Fc IgG recognizing the human Fc existed in plant leaf extracts. For blocking buffer conditions, 3% BSA buffer clearly blocked the plate, compared to the 5% skim-milk buffer. For capture antibody, monoclonal antibody (MAb) CO17-1A was applied to coat the plate with different amounts (1, 0.5, and 0.25 μg/well). Among the amounts of the capture antibody, 1 and 0.5 μg/well (capture antibody) showed similar absorbance, whereas 0.25 μg/well of the capture antibody showed significantly less absorbance. Taken together, the optimized conditions to quantify plant-derived GA733-FcK were 0.5 μg/well of MAb CO17-1A per well for the capture antibody, 3% BSA for blocking buffer, and anti-human Fc conjugated HRP. To confirm the optimized ELISA conditions, correlation analysis was conducted between the quantified amount of GA733-FcK in ELISA and its protein density values of different leaf samples in Western blot. The co-efficient value R(2) between the ELISA quantified value and protein density was 0.85 (p<0.01), which indicates that the optimized ELISA conditions feasibly provides quantitative information of GA733-FcK expression in transgenic plant.

How Plants Sense Wounds: Damaged-Self Recognition Is Based on Plant-Derived Elicitors and Induces Octadecanoid Signaling

BackgroundAnimal-derived elicitors can be used by plants to detect herbivory but they function only in specific insect–plant interactions. How can plants generally perceive damage caused by herbivores? Damaged-self recognition occurs when plants perceive molecular signals of damage: degraded plant molecules or molecules localized outside their original compartment.Methodology/Principal FindingsFlame wounding or applying leaf extract or solutions of sucrose or ATP to slightly wounded lima bean (Phaseolus lunatus) leaves induced the secretion of extrafloral nectar, an indirect defense mechanism. Chemically related molecules that would not be released in high concentrations from damaged plant cells (glucose, fructose, salt, and sorbitol) did not elicit a detectable response, excluding osmotic shock as an alternative explanation. Treatments inducing extrafloral nectar secretion also enhanced endogenous concentrations of the defense hormone jasmonic acid (JA). Endogenous JA was also induced by mechanically damaging leaves of lima bean, Arabidopsis, maize, strawberry, sesame and tomato. In lima bean, tomato and sesame, the application of leaf extract further increased endogenous JA content, indicating that damaged-self recognition is taxonomically widely distributed. Transcriptomic patterns obtained with untargeted 454 pyrosequencing of lima bean in response to flame wounding or the application of leaf extract or JA were highly similar to each other, but differed from the response to mere mechanical damage. We conclude that the amount or concentration of damaged-self signals can quantitatively determine the intensity of the wound response and that the full damaged-self response requires the disruption of many cells.Conclusions/SignificanceNumerous compounds function as JA-inducing elicitors in different plant species. Most of them are, contain, or release, plant-derived molecular motifs. Damaged-self recognition represents a taxonomically widespread mechanism that contributes to the perception of herbivore feeding by plants. This strategy is independent of insect-derived elicitors and, therefore, allows plants to maintain evolutionary control over their interaction with herbivores.

Evidence that a shoot-derived substance is involved in regulation of the super-nodulation trait in soybean

Results of grafting experiments between super-nodulation (or hyper-nodulation) mutants of soybean and their parents reconfirmed that super nodulation is a shoot-controlled phenomenon, suggesting that a systemic regulatory mechanism acts in soybean plants and a specific nodulation-controlling substance (SNS) is synthesized in the shoot and transported to the roots. To search for the SNS involved in the super-nodulation trait of NOD1-3, a mutant of soybean (Glycine max [L.] Merr. cv. Williams), we adopted a bioassay system using plantlets derived from the first trifoliate leaf of the seedlings; this system enabled us to introduce liquid substances continuously into leaves and to assess their effect on root nodulation. Following the application of leaf extract from Williams82 plants lacking visible root nodules, formation of root nodule meristems in NOD1-3 plantlets was repressed on the sixth day after rhizobial inoculation and the number of visible nodules on the eighth day declined to the same level as that in the Williams82 plantlets. Application of NOD1-3 leaf extract resulted in no significant change in the nodulation of both NOD1-3 and Williams82 plantlets. These results suggested that the SNS is a downregulator of nodulation and is responsible for the wild-type (Williams82) phenotype, and that the super-nodulation phenomenon is caused by a paucity of the SNS. The intensity of the repressive effect of the Williams82 leaf extract was not changed by nodulation of the source plants, thus we conclude that visible nodule formation is not required to induce production of the SNS.

Leaf and root extracts of the invasive shrub, Lonicera maackii, inhibit seed germination of three herbs with no autotoxic effects

In addition to effects mediated by resource competition, some invasive plants may impact surrounding vegetation by secreting compounds that are directly inhibitory to growth. Lonicera maackii, an invasive Asian shrub of forests and open areas in eastern and midwestern North America, has devastating effects on understory vegetation, some of which persist even after this shrub is removed. In this study, we explored the potential of aqueous extracts of the leaves and roots of this plant to inhibit seed germination of Impatiens capensis, Alliaria petiolata, Arabidopsis thaliana, and L. maackii in Petri dish bioassays. Both L. maackii root and leaf extracts significantly decreased germination in the three herb species. This inhibitory effect generally increased with increasing extract concentration and was more pronounced with application of leaf extract than root extract. However, when the same extracts were applied to seeds of L. maackii itself, germination was delayed in some cases, but was not significantly reduced by the end of the experiment. Germination of L. maackii seeds even reached significantly higher levels in some extract treatments than in no-extract controls. This implies that L. maackii can successfully inhibit the germination of other plants with few autotoxic effects and may even promote the germination of its own seeds.

Application of leaf extract causes repetitive action potentials inBiophytum sensitivum

A wound stimulus evoked a number of repetivite action potentials in the leaf ofBiophytum sensitivum. When the cut end of a leaf was immersed in a leaf extract, the resulting repetitive action potentials continue for a long time. These repetitive action potentials disappeared immediately when the leaf extract, which contains a proposed stimulant, was removed and the cut end was washed with water.