Application Of Indolebutyric Acid Research Articles

Effects of Nano Silver and Indole Butyric Acid Application on Growth and Some Physiological Characteristics on Hardwood Cutting of Dalbergia sissoo Roxb

Nanosilver (NS) and indole-3-butyric acid (IBA) can improve cutting performance and subsequent growth. This study was performed in Taqtaq city in a randomized complete block design to study the effects of NS (30, 60, and 90 mg/L) and IBA (50, 100, and 200 mg/L) in addition to distilled water as a control on growth and some characteristics of hardwood cutting of Dalbergia sissoo Roxb. Application of IBA enhanced significantly buds sprouting, where the cutting treated with 50 mg/L IBA sprouted after 35.53 days. IBA at 200 mg/L increased plant leaf area significantly to 204.11 dm2 in comparison to the control cuttings (122.00 dm2). Furthermore, IBA at 50 and 200 mg/L increased the number of leaves to 194.66 and 193.00 leaves/plant, compared to control cuttings (158 leaves/plant). The lowest peroxidase activity (902.00 and 903.30 absorbing units/ g fresh leaves) was observed in the cuttings soaked in 30 and 60 mg/L NS, respectively. Both NS and IBA had a significant effect on macro and microelements in the shoot except Mg and Fe. The shoot content of elements was different in response to NS and IBA applications, whereas the high level of IBA decreased significantly K content (25.80 %) it increased significantly the shoot content of Zn (0.22%). However the lowest concentration of NS (30 mg/L) decreased significantly the Cu content (0.02 %) and increased significantly the shoot content of Mn (0.58%). Root response to NS and IBA also was different, where 90 mg/L NS increased significantly each of K (26.40%) and Zn content (0.68 %), whereas it decreased significantly the root content of Fe (8.78%). The enhancement effects of IBA were more than that of NS on most studied characteristics.

Foliar application of Boron, Calcium, and Indole-3-butyric acid on onion plants to improve seed production

Onion is the third most important cultivated vegetable crop around the world. Two types of varieties are cultivated: Open pollinated cultivars (OP) and first generation hybrids (F1). Little is known about the effects of foliar applications on onion seed production. This study was conducted to evaluate the enhancement of onion seed yield and seed quality through foliar applications of Boron (B), Calcium (Ca), and an auxin, indole-3-butyric acid (IBA). Male-sterile lines (MSL), and OP planted in pots and open field conditions were used over four seasons. The experiments were carried out with different levels of nutrients and IBA applied to the plants. Our results showed that foliar applications of nutrients and plant growth regulators were effective methods to improve onion seed yield and the number of seed per fruit, while seed quality parameters remained stable. The seed yield was enhanced by B by up to 83 %, but with an increasingly wide variability in comparison to the control. Indole-3-butyric acid improved yields when it was applied alone and mixed with B and Ca (40–45 % for OP and MSL, respectively) compared to the control. Therefore, foliar application of IBA, B and Ca could be effective in improving onion seed yields.

Application of Indole Butyric Acid (IBA) to Improve the Quality of MD2 Pineapple Seedlings from Crown Shoots and Sapling Shoots

This aim of this research is to determine the effect of Indole Butyric Acid (IBA) concentration to the origin of the planting material and their interaction on the growth and development of Millie Dillard-2 (MD2) variety pineapple seedlings so that quality pineapple seedlings with good performance and rooting are obtained. The research was conducted from May to December 2023, experimentally using a factorial Randomized Block Design (RBD). The first factor is IBA concentration of 0 ppm, 200 ppm, 400 ppm, and 600 ppm. The second factor is the origin of the crown shoots and sapling shoots. Thus, there were eight treatment combinations; each treatment was repeated three times, so there were 24 experimental units. Each experimental unit used 3 plant samples. The Analysis of Variance (ANOVA) results were tested further using the Duncan’s Multiple Range Test (DNMRT) test at the 5% level. Observation were carried out on seedlings aged 30 Day After Planting (DAP) and 100 DAP with observation variables consisting of the root fresh weight aged 30 DAP and 100 DAP, total primary roots and increase in number of leaves. The research results show that giving a concentration of IBA of 400 ppm and seedling shoot planting material is the best treatment combination to be applied to MD2 pineapple seedlings.

Effect of two different base cuts and indole-3-butyric acid (IBA) doses on rooting of stem cuttings of wild Silene compacta Fischer

Silene compacta Fisch. ex Hornem., which grows naturally, is a plant species with high ornamental plant potential in the Silene L. genus of the Caryophyllaceae family. Vegetative propagation is one of the important ways to preserve naturally growing and cultivated plant species. In this study, the effect of two different cutting areas of the cutting base (above the node-below the node) and five different indole-3-butyric acid (IBA) doses (control, 500, 1000, 2000 and 4000 ppm) on the rooting of stem cuttings taken from individuals of the natural S. compacta species were investigated. IBA was applied to the cuttings’ bases in a commercial rooting powder. Perlite+peat mixture at a ratio of 1:1 (v/v) was used in the rooting medium. Survival rate, rooting percentage, blooming rate, root number, root length, seedling height, seedling stem diameter, side shoot number, shoot length and shoot stem diameter were evaluated. The evaluation was made one month after the start of the experiment. As a result, the highest survival rate in cuttings was obtained from above-node control group (100%) and under-node 2000 ppm IBA (95%) application, and the highest rooting percentage was obtained from under-node 2000 ppm IBA (68%) and above-node control group (65%), respectively. Based on the results, it can be concluded that different cuttings of the cutting base and IBA concentrations have different effects on the rooting success of the S. compacta species with stem cuttings.

Bee honey improved the performance of indole-3-butyric acid on promoting adventitious roots formation of Cupressus macrocarpa L. var. Goldcrest: Morpho-biochemical and histoanatomical investigation

The effective role of different auxins on adventitious roots (Ars) formation of stem cuttings relies on several factors including type of auxins, concentrations and the intermixing materials. In this study, bee honey (BH) was evaluated as a cofactor for maximizing the promoting effect of indole-3-butyric acid (IBA) for better survival of propagated hard-to-root ornamental plants. To improve Ars morphogenesis of Cupressus macrocarpa L. var. Goldcrest, a greenhouse experiment was conducted to evaluate the efficacy of exogenous application of IBA intermixed with BH in comparison with talcum powder (TP) on morphological, biochemical, histoanatomical, and ultrastructural responses. Results indicated that 500 mg kg-1 IBA intermixed with BH (T5) was the superior treatment for improving rooting percentage and root growth measurements. Exogenous application of T5 led to maximize activities of polyphenol oxidase and catalase enzymes as well as total phenols content; however, it caused a sharp decline in peroxidase enzyme activity as compared with untreated cuttings. Additionally, this treatment decreased amounts of gibberellin, benzyl adenine, kinetin and abscisic acid, while the endogenous content of indole-3-acetic acid increased. Histoanatomical observations of stem cuttings treated with T5 showed four prominent phases for adventitious root formation including: induction, initiation of meristemoids, root primordial formation and Ar elongation at 0–6th, 6-12th, 12-18th and 18-30th day after planting (dap). The Ar primordia evolved via two different developmental pathways: (i) indirect genesis (i.e., callus tissues) and (ii) direct genesis (e.g., cells in the inner bark, medullary rays and surrounding tissues). The time-course histoanatomical data of this study provide insights regarding the potential utilization of IBA intermixed with BH to improve Ar formation of several hard-to-root ornamentals.

Optimization of Rooting Behaviour by the Application of IBA on the Cuttings of Queens Flower (Lagerstroemia speciosa)

Background: Queen’s flower (Lagerstroemia speciosa) is an important ornamental plant that is utilised in residential landscape and avenue plantation for both public and private purposes around the world. Seed dormancy problem in Lagerstroemia which negatively affects germination. To overcome the challenges associated with seed propagation, researchers have explored rooted Lagerstroemia speciosa cuttings as an alternative method. Methods: Three concentrations of IBA (Indole-3- butyric acid) @ 1000 ppm, 2000 ppm and 3000 ppm were used to root initiation of Lagerstroemia speciosa stem cuttings. There were four treatments: 1000 ppm, 2000 ppm, 3000 ppm and control. Result: The 2000 ppm IBA treatment performed the better response in comparision to other treatments and recorded, 79 per cent rooting, followed by the treatment 3000 ppm IBA, (76 per cent rooting) and in the control, (68 per cent). In 2000 ppm IBA, the percentage of rooting, the number of roots per cutting and the length of the longest roots all are at their peak as well as showed the superior growth performance. The growth parameters of IBA-@ 2000 ppm treated cuttings revealed that this is the optimum IBA concentration for Lagerstroemia speciosa propagation trough cuttings in a semi-arid climate.

Assessment of the Responses of Chickpea (Cicer arietinum L.) to the Exogenous Application of Gibberellic Acid and Indole Butyric Acid at Different Crop Development Stages

Background: Chickpea (Cicer arietinum L.) is one of the most important pulse crops in the world. In Tunisia, the chickpea is vulnerable to fluctuation in production in the last few decades. However, phytohormones are known to play crucial roles in regulating different development processes in plants. Methods: This research was conducted in Petri dish and pot experiments in order to determine the effect of gibberellic acid (GA3) and indole butyric acid (IBA) phytohormones on some growth parameters of chickpea. In this study, GA3 at concentrations of 0, 0.05, 0.1, 0.15 and 0.2 g/l and IBA at concentrations of 0, 10-5, 10-4, 10-3 and 10-2 g/l were applied at different stages of chickpea development. Result: Results of Petri dishes trial revealed that IBA (10-4g/l) and GA3 (0.2 g/l) hormones improved significantly the shoot and root length of chickpea plant. Results of pots pointed out that the chickpea achieves the highest plant height when IBA (10-4 or 10-3 g/l) and GA3 (0.2 g/l) were used at pre-flowering stage. The IBA and GA3 application with concentrations of 10-3 g/l and 0.1 g/l, respectively allowed the highest nodule number at post-flowering stage. The parameters number of branches, fresh and dry weight of the aerial part and leaves number were improved in chickpea plants treated with IBA and GA3 at pre-flowering and post-flowering, respectively. Similarly, the number of flowers was promoted by the two hormones. The weight and the number of seeds were significantly enhanced by all IBA treatments in plants. An increase in weight and the number of seeds was observed at post-flowering phase of GA3 treatment. Thus, these results identified the beneficial effect of tested phytohormones in chickpea growth.

The changes in the maize root cell walls after exogenous application of auxin in the presence of cadmium.

Cadmium (Cd) is a transition metal and hazardous pollutant that has many toxic effects on plants. This heavy metal poses a health risk for both humans and animals. The cell wall is the first structure of a plant cell that is in contact with Cd; therefore, it can change its composition and/or ratio of wall components accordingly. This paper investigates the changes in the anatomy and cell wall architecture of maize (Zea mays L.) roots grown for 10 days in the presence of auxin indole-3-butyric acid (IBA) and Cd. The application of IBA in the concentration 10-9 M delayed the development of apoplastic barriers, decreased the content of lignin in the cell wall, increased the content of Ca2+ and phenols, and influenced the composition of monosaccharides in polysaccharide fractions when compared to the Cd treatment. Application of IBA improved the Cd2+ fixation to the cell wall and increased the endogenous concentration of auxin depleted by Cd treatment. The proposed scheme from obtained results may explain the possible mechanisms of the exogenously applied IBA and its effects on the changes in the binding of Cd2+ within the cell wall, and on the stimulation of growth that resulted in the amelioration of Cd stress.

The effect of different IBA doses on rooting in soft-wood cuttings of rootstock candidate sweet cherry, sour cherry and mahaleb genotypes

This study was carried out to investigate the effect of different IBA (Indole Butyric Acid) concentrations on rooting in soft-wood cuttings of rootstock candidate sweet cherry, sour cherry, and mahaleb genotypes in a plastic greenhouse with underfloor heating and mist propagation of Black Sea Agricultural Research Institute. 500, 1000, and 2000 ppm IBA doses were applied to the soft-wood cuttings taken in june. Perlite was used as propagation medium and rooting medium was disinfected with methyl bromide before planting. The study was planned according to a randomized plot design with three replications and 20 cuttings in each replication. The rooting rate (%), number of roots (piece), number of branching roots (piece), and root length (mm) were investigated. The highest rooting ratio of 59 % (08 K 056) was obtained from the 1000 ppm IBA application, while the lowest rooting ratio of 22.50% (55 K 104) was obtained from the 1000 ppm IBA application.

Phytoremediation Potential of <em>Ceratophyllum</em> sp. on Arsenic-Contaminated Conditions

Purpose: Aquatic contamination with arsenic is a serious problem as people will be at risk of arsenic toxicity when using and drinking contaminated water. Phytoremediation is a possible way to remove arsenic from contaminated water; however, arsenic is usually toxic to aquatic plants and decreases the phytoremediation efficiency. Alleviating the toxicity of arsenic to plant growth may be possible. Thus, increasing the phytoremediation capacity to decontaminate arsenic-contaminated water, aided by plant growth regulators, is a challenge. Thus, this study aimed to increase the potential of Ceratophyllum sp. to remove arsenic from contaminated water when plant growth regulators were added. Research Method: Ceratophyllum sp. was cultured in 2.50mg/L of arsenic-contaminated water for five days. The experiment was performed in a completely randomized design with one factor and five treatments (no plant growth regulator, salicylic acid, indole butyric acid, salicylic acid + calcium chloride and indole butyric acid + calcium chloride). Then the growth of Ceratophyllum sp., arsenic concentration in the plant biomass and the arsenic remaining in the water was determined at the end of the experiment. Findings: The use of salicylic acid and salicylic acid + calcium chloride tended to increase the fresh and dry weight of Ceratophyllum sp. grown in arsenic-contaminated conditions, but both formulas of plant growth regulator did not increase the arsenic accumulation in the biomass of Ceratophyllum sp. Application of indole butyric acid in combination with calcium chloride tended to increase the accumulation of arsenic in the biomass of Ceratophyllum sp. so it was 121.9mg/kg, but the bioconcentration factor was only 39.2. The application of salicylic or indole butyric acid tended to increase the removal of arsenic from contaminated water; however, the amount of arsenic remaining under applications of both plant growth regulators was not significantly different from that without plant growth regulator application. Research Limitation: There was no research limitation in this study. Originality/ Value: Based on the results in this study, Ceratophyllum sp. had no potential to remove arsenic from contaminated water and the plant growth regulator used in this study was not necessary to be used in the stimulation of arsenic removal.

The effects of growth regulatory agents in varying doses on Lavandula angustifolia and Lavandula × intermedia species in different rooting media

Lavender is a valuable ornamental and aromatic plant species with several applications, including food additives, cosmetics, perfumes, medicine, and aromatherapy, owing to its antimicrobial, antibacterial, antiviral, and antioxidant properties, as well as its pleasant odor. The purpose of this study was to determine the rooting capability of lavender species (Lavandula angustifolia and Lavandula × intermedia) in different media (peat, perlite, peat+perlite) and different doses of indole-3-butyric acid (IBA) (1000, 1500, and 2000 ppm) on cuttings. After 5 seconds in the (IBA) solution, the cuttings were planted in various rooting media in the greenhouse environment. The highest rooting rate, 93%, was obtained from 2000 ppm IBA application in peat+perlite (1:1) medium, whereas the lowest rooting rate, 13%, was obtained from Lavandula angustifolia species from control application in peat media. In a peat+perlite (1:1) medium with 1500 ppm, the longest root length was 13.16 cm. IBA application Lavandula angustifolia species, the lowest root length in Lavandula × intermedia cuttings were 2.7 cm from control application in perlite medium. Lavandula × intermedia cuttings in peat medium produced the best results in terms of root number (3.36 pcs/cuttings), while Lavandula × intermedia cuttings in perlite medium produced the lowest root number in the control application. In terms of viability rate, both species received high values, with the lowest viability rate obtained from the control application in perlite medium with 80% in Lavandula angustifolia species.

ASSESSING THE IMPACT OF INDOLE-3-BUTYRIC ACID (IBA) ON ROOTING EFFICIENCY IN CARNATION (DIANTHUS CARYOPHYLLUS L.) PROPAGATION UNDER PROTECTED CONDITIONS

The study aimed to assess the impact of varying Indole-3-Butyric Acid (IBA) concentrations on the rooting efficacy of Carnation (Dianthus Caryophyllus L.) cuttings within a controlled greenhouse setting. Different concentrations of IBA were applied to selected Carnation cultivars to stimulate root development. The greenhouse environment maintained controlled conditions, regulating temperature, humidity, and light to mimic optimal growth settings. The study focused on observing and analyzing rooting responses, particularly concerning root initiation, elongation, and biomass accumulation. Results indicated a distinct relationship between IBA concentrations and the rooting efficiency of Carnation cuttings. Lower concentrations displayed negligible effects on root development, while moderate concentrations significantly stimulated root initiation and elongation. Surprisingly, excessively high IBA concentrations led to reduced rooting responses, suggesting a hormetic effect on root growth. Morphological assessments highlighted that optimal IBA concentrations fostered vigorous root systems characterized by increased length, number, and mass of roots. This research underscores the pivotal role of precise IBA application in promoting successful rooting of Carnation cuttings, especially in controlled environments. The findings provide crucial insights for refining propagation techniques tailored specifically for Carnations, offering guidance to growers in optimizing IBA concentrations for efficient and sustainable cultivation practices in the floriculture industry. Further investigations delving into the prolonged impacts of IBA on overall plant growth and development would deepen the understanding derived from these findings.

Exogenous IBA stimulated adventitious root formation of Zanthoxylum beecheyanum K. Koch stem cutting: Histo-physiological and phytohormonal investigation

Poor rooting performance of Chinese pepper (Zanthoxylum beecheyanum K. Koch) shrub is a restriction for its commercial production. This investigation was carried out to assess the effectiveness of the exogenous application of indole-3-butyric acid (IBA) on adventitious root (AR) formation and some morpho-physiological, biochemical, and histological alterations associated with rhizogenesis events. IBA, at 1500 or 2000 mg kg-1, gave comparable effects for improving the performance of rooting architecture compared to the control treatment. The AR primordia of Z. beecheyanum originated from two distinct developmental pathways: (i) direct genesis from cells in the cambial zone and nearby tissues, and (ii) indirect genesis from cells in callus tissues. Rooting process involved four phases: (i) induction at 0–10 days after planting (DAP), (ii) initiation at 10–15 DAP, (iii) formation of the root primordia (Rp) at 15–20 DAP, and (iv) root extension at 20–30 DAP. Cuttings' inductive phase correlated temporarily with intensive cell divisions, high levels of endogenous indole-3-acetic acid (IAA) and gibberellins (GA3). However, IBA application led to a pronounced increase in soluble sugars and phenolic content. The initiation phase was accompanied by a histological appearance of meristemoids. This phase was also stimulated by the subsequent generation of low IAA and high GA3 contents. In the development of Rp and root extension phases, prominent Rp formation in the cambium and complete differentiation with vascular tissues continued with that of the stem cuttings. In essence, the current study provides insights into the morphological, histo-physiological, and phytohormonal alterations in stem cuttings.

The impact of auxin on the vegetative and reproductive growth of carrot (Dacus carota L.)

Seed is a basic component of every crop species and quality seed is essential for farmers to achieve greater crop production and higher yields. Seed yield can be improved by using the growth hormones. Indole-3-butyric acid (IBA) IBAplays a vital role in reproductive and seed development of crop plant. An investigation is being conducted to determine effect of rooting hormone IBA on reproductive growth that leads to seed developement. Roots treated with three levels of IBA (500 ppm, 1000 ppm and 1500ppm) along with control in a 3 replication. Results showed that roots treated with IBA @ 1500 ppm had taken least number (28.4) of days to flowering with maximum plant height (34.3cm), number of leaves (15.3), leaves length (19.6 cm), number of secondary (10) and tertiary umbels (13.7), diameter of secondary (12.7 cm) and tertiary umbel (4.4), weight of secondary (5.1 g) and tertiary umbel (3.5), seed weight of secondary (4.4 g) and tertiary umbels (1.5 g) and biological weight of the plants (81.0 g) respectively. However, the diameter, weight and seed weight of primary umbel was good in IBA @ 1000 ppm. Thus it is recommended that IBA application at concentration of 1500 ppm can significantly improve growth of carrot seed plants, hence increasing production and seed quality

IBA Delivery Technique and Media Salts Affected In Vitro Rooting and Acclimatization of Eight Prunus Genotypes

Difficult-to-root plants often perform poorly during acclimatization and in vitro rooting can increase the survival and quality of plants. The influence of auxin application and mineral nutrition on in vitro rooting and subsequent effects on plant quality in eight Prunus genotypes were investigated. Microshoots were rooted in vitro on Murashige and Skoog (MS), ½ MS, Driver and Kuniyuki (DKW), or New Prunus Medium (NPM) media formulations in combination with 15 µM indole-3-butyric acid (IBA), 4-day 15 µM IBA pulse, 1 mM 30 s quick-dip, or IBA-free treatments. Shoots were observed pre- and post-acclimatization to determine rooting methods to maximize quality and minimize labor. A genotype-specific response to auxin application was observed with seven of eight genotypes achieving 100% survival when paired with the recommended IBA treatment. Peaches performed best when treated with 4-day IBA pulse or 30 s quick-dip. Rooting of P. cerasifera, it's hybrid to P. persica, and P. munsoniana all benefitted from IBA application. Shoots rooted with 15 µM IBA were smaller and lower quality in most genotypes. DKW maximized size and quality in six genotypes. Better shoots and larger root systems during in vitro rooting produced better plants in the greenhouse with no detrimental effect of callus growth. Rooting techniques to maximize plant quality while reducing labor are specified.

Effect of Indole-3-Butyric Acid (IBA) on Performance of Mulberry (Morus alba L.) Saplings: A Review

The Mulberry (Morus sp.), is the food for silkworm and is of great importance to sericulture industry. It can be propagated by seeds, cuttings, budding and grafting. Propagation by cuttings has distinct advantages over the other methods due to high survival rate and quick establishment. By considering the importance of indole-3-butyric acid (IBA) in mulberry establishment, a review article has been formulated consisting of effect of IBA application on mulberry shoot and root parameters. The review of literature found that, the amount of use of IBA is considered as important by most of the researchers considering its role in root formation, increasing the root number and helps in mobilization of carbohydrates for root growth. Being an important crop for sericulture, the IBA application has significant economic impacts. This can be seen from increase in survival rate, number of leaves, length of shoot and rooting percentage of mulberry saplings.

Effect of Explant Age and Applied IBA on Growth and Rooting of Apical Stem Cuttings of Potato for Early Generation Seed Potato Production

The aim of this work is to study the effect of explant age and exogenously applied IBA on the growth and rooting potential of potato apical stem cuttings for production of first- generation seed potatoes. The experimental protocol consists in taking apical stem cuttings from potato plantlets at different physiological ages (30 and 60 days old) in interaction with the growth regulator indole-3-butyric acid (IBA) at different concentrations (100, 200, and 300 ppm) to study the production of potato minitubers. The results of the present study showed that mini-cuttings from young potato plants (30 days old) have a higher rooting capacity than mini-cuttings from mature plants. In addition, IBA concentrations, especially at 300 ppm, have a significant effect on most of the characteristics studied, e.g. rooting %, root number, root length, shoot length, and number of leaves. Regarding yield attributes, application of IBA at 300ppm gave the highest average tuber number (6.66) and tuber yield (142.97g) was higher in 30days old explant cuttings. The data presented in this study may encourage potato growers to use potato stem cuttings as a new, cheaper method of growing potatoes instead of expensive imported seed tubers. It is suggested that the use of apical stem cuttings can be a quick and effective method for mass propagation and to increase the yield of minitubers in the seed potato production cycle.

Effects of Naphthaleneacetic Acid, Indole-3-Butyric Acid and Zinc Sulfate on the Rooting and Growth of Mulberry Cuttings

The mulberry tree (Morus alba) is a perennial and fast-growing tree distributed worldwide under different climatic conditions. Most of the world’s silk production (>90%) is facilitated by the feeding of silkworm larvae on the leaves of mulberry (Morus alba L.) varieties. Therefore, exploration of the protocol for improving the propagation efficiency and increasing the reproductive capacity of M. alba varieties could be of great significance. This study aimed to determine the effect of four concentrations (0, 100, 200 and 400 mg L−1) each of naphthaleneacetic acid (NAA), indole-3-butyric acid (IBA), and zinc sulfate (0, 100 and 200 mg L−1), supplied separately or combined, on the rooting and growth of mulberry cuttings. M. alba cuttings were immersed for 5 s in each solution using the quick-dip method and subsequently, the cuttings were dried and planted in plastic pots and maintained in a greenhouse for 60 days. The number of leaves (NL), longest root size (LRS), longest stem size (LSS), number of rooted cuttings (NRC), number of stems per tree (NSP), rooting percentage (RP), wet root weight (WRW), dry root weight (DRW), wet stem weight (WSW), dry stem weight (DSW), wet leaf weight (WLW) and dry leaf weight (DLW) were evaluated. The results obtained showed an increase in all growth parameters of the mulberry cuttings. Treatments of hormones (IBA and NAA) and Zn sulfate were effective on LSS, LRS and WSW. The highest values of LSS were obtained for the treatments T5, T6, T14, T15, T16 and T18. Moreover, T5, T12 and T10 showed the highest values of LRS. The highest value of WSW was observed for T18, T5, T14, T15 and T16. The highest values of WLW and DLW were observed in T20 and T14. Dry stem weight (DSW) was high in T18 and T14. The application of NAA (at 200 mg L−1), IBA (200 and 400 mg L−1) and Zn sulfate (200 and 400 mg L−1), either alone or in double combination, can be a suitable and reliable method for mulberry propagation.

A foliar spray application of indole-3-butyric acid promotes rooting of herbaceous annual cuttings similarly or better than a basal dip

Foliar rooting hormone applications containing indole-3-butyric acid (IBA) to unrooted vegetative cuttings have gained popularity as they reduce labor costs and the risk of spreading diseases compared to standard basal dips. The objective of this study was to quantify if foliar applied 20% IBA promotes adventitious rooting of herbaceous annuals similarly to a basal dip application of IBA + 1-napthaleneacetic acid (NAA). Dahlia (Dahlia pinnata) Venti™ ‘Passion Fruit’, osteospermum (Osteospermum ecklonis) Serenity™ ‘Lavender Frost’, scaevola (Scaevola aemula) Scalora® ‘Brilliant’ and geranium (Pelargonium × hortorum) Sunrise™ ‘Lavender + Red Eye’ cuttings received a foliar spray of 0, 150, 300, or 600 mg·L–1 IBA at 0.2 L·m–2; 150 or 300 mg·L–1 IBA at 0.8 L·m–2; or a basal dip of 100 mg·L–1 + 50 mg·L–1 or 200 mg·L–1 + 100 mg·L–1 IBA + NAA. In most cases, cuttings receiving a foliar application of 300 mg·L–1 IBA at 0.8 L·m–2 had either comparable or greater root dry mass than cuttings receiving a basal dip of IBA + NAA and outperformed the control. After 14 d, root dry mass was generally greatest when cutting were treated with 300 mg·L–1 IBA at 0.8 L·m–2 and was an equal or better alternative to a basal dip. For the most effective results, rooting hormones should be utilized when the DLI integral is <12 mol·m–2·d–1.

Indole-3-butyric acid, an alternative to GA3 for bunch quality enhancing of table grape Vitis vinifera L. cv. Superior Seedless

Gibberellic acid (GA3) is the most widely used plant growth regulator to thin out bunches and increase berry size in seedless table grapes, but there is evidence of its negative effects, including loss of fertility and malformations of the rachis. This study analyses the effects of applying the auxin indole-3-butyric acid (IBA), as a novel alternative, on fruit yield, bunch structure, anatomy and postharvest quality of cv. Superior Seedless. The application of IBA and GA3 at different phenological stages (15 cm shoot length, full bloom, fruit set and pea-size berry) and doses (2, 20 and 50 ppm) were compared in a two-growing season experiment. Spraying IBA at full bloom or fruit set, improved bunch weight by increasing the size and weight of the berries, correlating with the promotion of rachis vascular tissues. Bunches treated with IBA retained a greater number of berries at harvest without generating compactness, since the elongation of the rachis internodes and lateral shoulders were also promoted. In addition, IBA augmented postharvest quality of bunches by reducing rachis browning and increasing berry firmness. These results suggest that the use of IBA is a beneficial technology to improve bunch structure and quality in seedless grapes. Highlights IBA effects applied at different dosages and phenological stages was evaluated. IBA at full bloom and fruit set improved bunch and berry weight and berry size. The number of berries per bunch increased with IBA at full bloom and fruit set. IBA increased lateral rachis internode and lateral rachis length via promotion of vascular tissues. IBA increased postharvest quality, reduced rachis browning and increased of berry firmness.